www.dft.uem.br/wsng

FINAL REPORT

We have successfully completed the Workshop: “Neurogenesis: Basic aspects and implications on neuropsychiatric conditions” with enthusiastic discussions and presentations by students, researchers and professors.

The Workshop: “Neurogenesis: Basic aspects and implications on neuropsychiatric conditions” take place at the University of Maringá in Maringá, Paraná, Brazil, from 3rd to 5th August 2015.

We received about 49 applications as answers to the announcement of the Workshop (please see list of participants). We had graduate students, post-graduate students, researchers and professors from several Brazilian provinces such as: Rio de Janeiro, Minas Gerais, São Paulo, Paraná, Santa Catarina. From all applications, 25 participants were selected to participate of the seminars that occurred in the afternoon period. The selected candidates presented a very nice letter of interest focusing in their need to better understanding the process of adult neurogenesis in order to include or to propose some related experiments in their thesis’ or research’s projects.

After the opening of the Workshop with the presence of the Rector of our University Dr Mauro Baeso, Dr Rúbia Maria W. de Oliveira (State University of Maringá, Brazil) spoke about the basic aspects of adult neurogenesis. Her talk was followed by Dr Ismael Galve-Roperh (Universidad Complutense, Madrid) and Dr Cilene Lino de Oliveira (Federal University of Santa Catarina, Brazil), who introduced topics related to neurogenic niches and adult neurogenesis regulation (please see the program below). After lunch the participants had the opportunity of presenting their results in a poster session (poster list below). The abstracts were published in the Anals of Workshop: Neurogenesis: Basic aspects and implications on neuropsychiatric conditions, ISBN 978-85-66182-02-6. In the same day, the 25 selected students were divided in four (4) groups for seminar presentations. Each group had an expert in the field of the seminar to help them with the seminars.

Three participants (Taysa Bassani/Federal University of Paraná, Lígia Mendes Soares/State University of Maringá and Victor Rodrigues Santos/University of São Paulo) were selected accordingly their letters and research projects to present orally their experimental results. The oral presentation occurred before the afternoon seminars when very interesting and productive discussions taken place followed by the consultancy of the experts in the area.

In the second day, Dr Alline C. Campos (University of São Paulo, Brazil) and Dr Lorena Varela Nallar (Universidad Andres Belo, Chile) talked about neurogenesis and neuropsychiatric conditions such as stress and Alzheimer’s disease. Dr Roberto Andreatrini (Federal University of Paraná, Brazil) followed their presentations giving us a clinical and translational view of the experimental findings and the importance of the neurogenesis in mania and depression. At night, local students prepared a very nice party with folk music and typical Brazilian food.

In the last day, Dr Gabriel Arisi (Federal University of São Paulo, Brazil) highlighted the morning with an interesting talk about methods of microscopy quantification focusing in stereology and clarity.

After the seminars in the afternoon, we had a quick evaluation of the Workshop and the participants ranked their global experience as positive an informative with clear cut effects in their future scientific works. Please see in attach the pictures.

1. LECTURERS

Prof. Dr. Alline Cristina de Campos – Medical School, Department of Pharmacology, University of São Paulo, Ribeirão Preto, Brazil.

Prof. Dr. Cilene Lino de Oliveira – Federal University of Santa Catarina, Department of Physiology, SC, Brazil.

Prof Dr Gabriel Arisi – Federal University of São Paulo, Laboratory of Neurobiology, São Paulo, Brazil.

Prof Dr Ismael Galve-Roperh - Universidad Complutense de Madrid, Departamento de Bioquimica y Biologia Molecular, Madri, Espanha.

Prof. Dr. Lorena Varela-Nallar- Universidad Andres Belo, Centro de Investigaciones, Santiago, Chile

Prof Dr Roberto Andreatini – Federal University of Paraná, Department of Pharmacology, Curitiba, Paraná, Brazil.

Prof. Dr. Rúbia Maria Weffort de Oliveira – State University of Maringá, Department of Pharmacology and Therapeutics, Maringá, Paraná, Brazil.

2. PROGRAM AT A GLANCE

3. LIST OF PARTICIPANTS (MORNING LECTURES AND POSTER PRESENTATION)

1. Amanda Nunes Santiago PhD student2. Ana Paula Farias Waltrick Graduation student3. Angélica Pupin Schiavon Researcher4. Betania Braga da Silva Graduation student5. Bruna de Sá Barbosa Master student6. Bruno Ambrósio da Rocha PhD student

Workshop: “Neurogenesis: basic aspects and implications

on neuropsychiatric conditions”

03/08/2015 04/08/2015 05/08/2015

8:30-9:00 h Oppening

9:00-9:50 hRúbia W. Oliveira

“Neurogenesis: a complex multi-step

process”

Alline Campos

“Behavioral implication of adult neurogenesis

during stress responses”

Ismael G. Roperh

“Pharmacological manipulation of adult

neurogenesis”

9:50-10:40 hIsmael G. Roperh

‘Neural progenitor cells in adult neurogenic

niches”

Lorena Varela-Nallar

“Adult neurogenesis in Alzheimer’s disease”

Gabriel Arisi

“Clarity and stereology on quantitative analysis of adult neurogenesis”

10:40-11:00 h Coffee break Coffee break Coffee break

11:00- 11:50 hCilene L. Oliveira

“Environment influence on adult neurogenesis”

Roberto Andreatini

“Implications of adult neurogenesis in mood

disorders: focus on mania”

Alline Campos

“Would neurogenesis be a

possible target for the treatment of

neuropsychiatric disorders ?”

12:00-13:30 h Lunch Lunch Lunch

13:30-15:00 hPoster presentation

(29 posters)

Coffee break

Oral presentation (1)

Seminar 1In vitro and in vivo

protocols for analysis of adult neurogenesis

Oral presentation (2)

Seminar 2In vitro and in vivo

protocols for analysis of adult neurogenesis

Oral presentation (3)

Seminar 3In vitro and in vivo

protocols for analysis of adult neurogenesis

Seminar 4In vitro and in vivo

protocols for analysis of adult neurogenesis

15:00-16:00 h

16:00- 18:00h Time to students welcome and study

period (seminar preparations)

7. Caio Cesar Sestile PhD student8. Camila Fernanda Estevão da Silva PhD student9. Cristiano Correia Bacarin PhD student10. Ciomar Ap. Bersani Amado Researcher/Professor11. Danielle Rodrigues de Souza PhD student12. Debora de Mello Gonçales Sant´Ana Researcher/Professor13. Emilene Dias Fiuza Ferreira Researcher14. Erika Meyer Master student15. Fernanda Navarro Marin Master student16. Fernanda Troyner Master student17. Gabriel Gripp Fernandes Master student18. Gabriel Roldi Geraldo Master student19. Gabriela Travençolo Master student20. Gislaine Cardoso de Souza Master student21. Jacqueline Godinho PhD student22. Humberto Milani Researcher/Professor23. Jéssica Mendes Bonato Master student24. Jessica Rosa PhD student25. Jhonatan Christian Maraschin PhD student26. Juliana dos Reis Bastos Master student27. Letícia Pin Coltri Master student28. Ligia dos Santos Mendes Lemes Soares PhD student29. Lorena Gimenez da Silva PhD student30. Luane Oliveira Reis Graduation student31. Lucas Gazarini Researcher32. Manuela Torrado Truiti Graduation student33. Marco Aurélio Mori PhD student34. Maria Aparecida Barbato Frazão Vital Researcher/Professor35. Marina Aparecida Magnini Portes PhD student36. Marina Masetto Antunes Graduation student37. Naielly Rodrigues da Silva Master student38. Natalia Yumi Batista Tamura Master student39. Nilson Carlos Ferreira Junior Researcher40. Rafael Aguiar Master student41. Rafael Scoz Silva Master student42. Silvana Regina de Melo Researcher/Professor43. Taketoshi Sakurada Junior Graduation student44. Taysa Bervian Bassani PhD student45. Thais Emanoelle Tavares Pompeu PhD student46. Tiago Guardia de Souza e Silva PhD student47. Victor Rodrigues Santos Researcher48. Vivian Suemi Batista Tamura Graduation student49. Vivian Taciany Bonassoli Researcher

4. LIST OF SELECTED STUDENTS FOR THE SEMINARS (AFTERNOON)

1.Amanda Nunes Santiago2.Bruno Ambrósio da Rocha

3.Caio Cesar Sestile4.Cristiano Correia Bacarin

5.Emilene Dias Fiuza Ferreira

6.Erika Meyer7.Fernanda Navarro Marin

8.Fernanda Troyner9.Gabriel Gripp Fernandes

10.Jacqueline Godinho11.Jéssica Mendes Bonato

12.Jessica Rosa13.Jhonatan Christian Maraschin

14.Juliana dos Reis Bastos15.Ligia dos Santos Mendes Lemes Soares

16.Marco Aurélio Mori17.Marina Aparecida Magnini Portes

18.Naielly Rodrigues da Silva19.Nilson Carlos Ferreira Junior

20.Rafael Scoz Silva21.Taysa Bervian Bassani

22.Thais Emanoelle Tavares Pompeu23.Tiago Guardia de Souza e Silva

24.Victor Rodrigues Santos25.Vivian Taciany Bonassoli

5. POSTERS’ PRESENTATIONS

1. HYPERGLYCEMIA IMPAIRS COGNITION IN MIDDLE-AGED RATS SUBJECTED TO CHRONIC CEREBRAL HYPOPERFUSION. Amanda N. Santiago, Emilene D. F. Ferreira, Humberto Milani and Rúbia M. W. de Oliveira. State University of Maringá, Department of Pharmacology and Therapeutics, Maringá, Paraná, Brazil.

2. REPEATED CANNABIDIOL TREATMENT ENHANCES CELL PROLIFERATION AND NEUROGENESIS IN THE HIPPOCAMPAL SUBGRANULAR ZONE AND SUBVENTRICULAR ZONE. Schiavon AP1, Bonato JM1, Milani H1, Guimarães FS2, de Oliveira RMMW1. 1 Department of Pharmacology and Therapeutics, State University of Maringá, Paraná. 2 Department of Pharmacology, Medicine Faculty of Ribeirão Preto/São Paulo University

3. COMPARATIVE EFFECT OF CURCUMIN IN NATURA AND CURCUMIN NANOPARTICLE ON EXPERIMENTAL INFLAMMATORY RESPONSE. Bruno Ambrósio da Rocha 1 , Edirlene Sara Wisniewski Rebecca1, Odinei Hess Gonçalves2, Fernanda Vitória Leimann2, Ciomar Aparecida Bersani-Amado1. 1 Department of Pharmacology and Therapeutics, State University of Maringá, Maringá-PR, Brazil. 2 Department of Food Technology, Federal Technological University of Paraná, Campo Mourão-PR, Brazil.

4. COOPERATION BETWEEN 5-HT1A AGONIST AND k-OPIOID ANTAGONIST: ANXIOLYTIC AND PANICOLYTIC-LIKE EFFECTS IN ANIMAL MODELS. Caio César Sestile 1 , Marcel Pereira Rangel1, Jhonatan Christian Maraschin1, Mariana Kitayama1, Camila Marroni Roncon2, Frederico Guilherme Graeff2, Hélio Zangrossi JR2, Elisabeth Aparecida Audi1. 1 State University of Maringá, Maringá – PR. 2 University of São Paulo, Ribeirão Preto – SP.

5. MICROTUBULE-ASSOCIATED PROTEIN-2 (MAP2) EXPRESSION AFTER TRANSIENT, GLOBAL CEREBRAL ISCHEMIA IN RATS AND THE INFLUENCE OF FISH OIL-TREATMENT. Cristiano Correia Bacarin, Rúbia Maria Weffort de Oliveira, Humberto Milani. Department of Pharmacology and Therapeutics, State University of Maringá, Maringá-PR, Brazil.

6. Expression of glial cells and inflammatory cytokines after transient global cerebral ischemia: Fish oil effects. Ferreira, E.D.F.; Romanini, C.V.; de Oliveira, R.M.M.W.; Milani, H. Department of Pharmacology, State University of Maringá, Paraná, Brazil.

7. ANTIDEPRESSANT-LIKE EFFECTS OF MYRICITRIN AND ITS RELATIONSHIP TO ADULT NEUROGENESIS IN MICE. Meyer E 1 , Mori MA1, Andreatini, R2, Milani H1, Oliveira RMW1. 1 Department of Pharmacology and Therapeutics, State University of Maringá, Paraná. 2 Department of Pharmacology, Federal University of Paraná.

8. CHARACTERIZING/UNDERSTANDING THE FEAR GENERALIZATION PHENOMENON. Fernanda Navarro Marin 1 , Leandro José Bertoglio1. 1. Department of Pharmacology. Federal University of Santa Catarina, Biological Sciences Center, Florianopolis, Brazil.

9. THALAMIC NUCLEUS REUNIENS AND VENTRAL HIPPOCAMPUS: PROPOSED MODULATION OF FEAR MEMORY SPECIFICITY. Fernanda Troyner 1 , Leandro José Bertoglio1. 1. Department of Pharmacology. Federal University of Santa Catarina, Biological Sciences Center, Florianopolis, Brazil.

10. INVOLVEMENT OF THE DORSAL PERIAQUEDUCTAL GRAY IN THE CONTROL OF PANIC-LIKE DEFENSIVE BEHAVIORS ELICITED BY HYPOXIA IN RATS. Gabriel G. Fernandes, Ailton Spiacci Jr, Hélio Zangrossi Jr. School of Medicine of Ribeirao Preto, University of Sao Paulo, Brazil.

11. CILOSTAZOL BUT NOT SILDENAFIL REVERTS CHRONIC CEREBRAL HYPOPERFUSION-INDUCED MEMORY DEFICIT IN RATS. Godinho, J; Bacarin CC; Ferreira, EDF; Zaghi, GGD; Oliveira, JN; Oliveira, RMW, Milani, H. Department of Pharmacology and Therapeutics, State University of Maringá. Maringá / PR.

12. Depressive-like behavior displayed by mice following cannabidiol withdrawal: preliminary results. Jéssica Mendes Bonato, Angélica Pupin Schiavon, Humberto Milani and Rúbia M. Monteiro Weffort de Oliveira. State University of Maringá, Department of Pharmacology and Therapeutics, Maringá, Paraná, Brazil.

13. THE EFFECT OF ACUTE STRESS AND THE ROLE OF MINERALOCORTICOID AND GLUCOCORTICOID RECEPTORS PRESENT IN THE PREFRONTAL CORTEX IN THE MEMORY EXTINCTION LEARNING. Jessica Rosa, Daniela Lescano Martins Uliana, Leandro Antero da Silva, Leonardo Barbosa Moraes Resstel. Neuropharmacology Laboratory, Faculty of Medicine, Pharmacology Department, University of São Paulo. Ribeirão Preto, São Paulo, Brazil.

14. INHIBITION OF OLIGOPEPTIDASES CAUSES PANICOLYTIC-LIKE EFFECT MEDIATED BY µ-OPIOID RECEPTORS IN RATS. 1 Jhonatan Christian Maraschin, 1Marcel Pereira Rangel, 1Antonio Joaquim Bonfim, 1Mariana Kitayama, 2Frederico Guilherme Graeff, 2,3Hélio Zangrossi Jr., and 1,2Elisabeth Aparecida Audi. 1. Department of Pharmacology and Therapeutic, State University of Maringá, Maringá, Brazil. 2. Institute of Neurosciences and Behavior (INeC), Ribeirão Preto, Brazil. 3. Department of Pharmacology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.

15. INHIBITION OF DOPAMINE TRANSPORTER INDUCES MANIA-LIKE BEHAVIOUR AND ALTERS CYTOKINE LEVELS IN THE PREFRONTAL CORTEX AND HIPPOCAMPUS. Juliana R. Bastos 1,2,4 ,

Aline S. Miranda3,4, Sâmia R. L. Joca5, Antônio L. Teixeira3,4, Fabrício A. Moreira1,4. 1. Laboratory of Neuropsychopharmacology, Department of Pharmacology, ICB; 2. Graduate Program in Neuroscience; 3. Interdisciplinary Laboratory for Medical Research , FM; 4. Federal University of Minas Gerais – UFMG, BH, MG, Brazil. 5. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo - USP.

16. CHRONIC ROLIPRAM TREATMENT INCREASES THE EXPRESSION OF NEURAL PLASTICITY MARKERS IN MICE SUBJECTED TO BILATERAL COMMON CAROTID ARTERY OCCLUSION. L.M. Soares 1 , Jochen De Vry2, J Prickaerts2, H Milani1 and R.M.W. de Oliveira1. 1. State University of Maringá, Department of Pharmacology and Therapeutics, Maringá, Paraná, Brazil. 2. University of Maastricht, Department of Psychiatry and Neuropsychology, Maastricht, Netherlands.

17. BRAIN LACTATE ALTERATIONS IN RATS SUBMITED TO FORCED SWIMMING. Patrícia Batista Travassos1, Roberto Barbosa Bazotte1, Marina Masetto Antunes1, Vanessa Rodrigues Vilela1, Any de Castro Ruiz Marques1, Tuane Krupek1, Lorena Gimenez da Silva 1 . 1. Department of Pharmacology and Therapeutics, State University of Maringá - UEM, Maringá, Paraná, Brazil.

18. OBJECT LOCATION MEMORY IMPAIRMENTS IN RATS SUBJECTED TO TRANSIENT GLOBAL ISCHEMIA: EFFECTS OF FISH OIL. L.O. Reis, J.N. Oliveira, L.M. Soares, E.D.F. Ferreira, R.M.W. Oliveira, H. Milani. Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, Paraná, Brazil.

19. T. CATIGUA ETHYL ACETATE FRACTION PREVENTS ANXIETY-LIKE BEHAVIORS INDUCED BY TRANSIENT BRAIN ISCHEMIA IN MICE. M. T. Truiti, J.P.C. Ferreira, L.M. Soares, João Carlos P. Mello, Humberto Milani and R.M.W. de Oliveira. State University of Maringá, Department of Pharmacology and Therapeutics, Maringá, Paraná.

20. CANNABIDIOL AMELIORATES FUNCTIONAL OUTCOME AND INCREASES HIPPOCAMPAL NEUROGENESIS IN ISCHEMIC MICE. M.A. Mori 1, E.Meyer1, L.M. Soares1, H Milani1, F. Guimarães2

and R.M.W. de Oliveira1.1. State University of Maringá, Department of Pharmacology and Therapeutics, Maringá, Paraná, Brazil. 2. Faculty of Medicine of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.

21. STRENGTHENING AN AVERSIVE MEMORY: SYSTEMIC OR PRELIMBIC CORTEX ENHANCED NORADRENERGIC ACTIVITY INDUCES GENERALIZED FEAR EXPRESSION. Marina Aparecida Magnini Portes 1 ; Ana Carolina Vanvossen 1; Lucas Gazarini 1; Karoline Batista Leite 1; Leandro José Bertoglio 1. 1. Department of Pharmacology, CCB, Federal University of Santa Catarina, Florianopolis, Brazil.

22. A COMPARARISON OF THE EFFECTS OF ORAL GLUCOSE AND LACTATE IN MICE SUBMITTED TO INSULIN-INDUCED HYPOGLYCEMIA. Vanessa Rodrigues Vilela1, Roberto Barbosa Bazotte1, Lorena Gimenez da Silva1, Patrícia Batista Travassos1,Any de Castro Ruiz Marques1, Tuane Krupek1, Marina Masetto Antunes 1 . 1. Department of Pharmacology and Therapeutics, State University of Maringá - UEM, Maringá, Paraná, Brazil.

23. EVALUATION OF THE EFFECTS INDUCED BY ACUTE AND REPEATED CANNABIDIOL TREATMENT ON BEHAVIORAL CHANGES IN A MODEL OF SCHIZOPHRENIA BASED ON ANTAGONISM OF NMDA RECEPTORS. Rodrigues, N.S. 1 ; Silva, N.R1; Gomes, F.V1; Guimarães, F.S1. 1. Department of Pharmacology, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.

24. ROLE OF DORSAL HIPPOCAMPUS ON BAROREFLEX ACTIVITY: INVOLVEMENT OF GLUTAMATE/ ENDOCANNABINOIDS AND ACETYLCHOLINE/ NITRIC OXIDE PATHWAYS. Ferreira-Junior NC, Lagatta DC, Resstel LBM. Department of Pharmacology, USP. Ribeirao Preto.

25. INFLUENCE OF CIRCADIAN RHYTHM ON THE EFFECTS OF CB1R ANTAGONISM ON THE CONSOLIDATION OF CONTEXTUAL FEAR MEMORY. Scoz-Silva R, Gazarini L and Bertoglio LJ. Department of Pharmacology, CCB, UFSC, Florianópolis, SC, Brazil.

26. COGNITIVE DECLINE IN THE STREPTOZOTOCIN-INDUCED MODEL OF ALZHEIMER’S DISEASE IS RELATED TO IMPAIRMENT IN HIPPOCAMPAL NEUROGENESIS. Taysa Bervian Bassani 1 , Meira M. F. Machado1, Jéssica M. Bonato2, Jacqueline Godinho2, Rúbia M. W. Oliveira2 and Maria A. B. F. Vital1. 1. Department of Pharmacology, Federal University of Paraná, Curitiba PR, Brazil. 2. Department of Pharmacology, State University of Maringá, Maringá, PR, Brazil.

27. EVALUATION OF THE MECHANISM OF ACTION OF LASSBio-579, AN ATYPICAL ANTIPSYCHOTIC DRUG CANDIDATE AND ITS p-HIDROXYLATED METABOLITE: INVOLVEMENT OF β-arrestin2/Akt/GSK-3β PATHWAY THROUGH D2 RECEPTOR. Pompeu, Thais Emanoelle Tavares 1 ; Noël, François1. 1. Lab Farmacologia Bioquímica e Molecular, ICB, UFRJ.

28. EVALUATION OF NEUROPROTECTIVE AND BEHAVIORAL EFFECTS OF TREATMENT WITH ROYAL JELLY ON ANIMAL MODEL OF SPORADIC ALZHEIMER'S DISEASE. Silva,TGS 1 ; Britto,LRG2; Xavier,GF3; Sandoval,MRL4. 1. Institute of Psychology, University of São Paulo, São Paulo, Brazil. 2. Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. 3. Institute of Biosciences, University of São Paulo, São Paulo, Brazil. 4. Pharmacology Laboratory, Butantan Institute, São Paulo, Brazil.

29. ABNORMAL MORPHOLOGICAL AND PHYSIOLOGICAL ALTERATIONS OF NEWBORN NEURONS IN HIPPOCAMPUS IS CAPABLE OF CAUSING EPILEPSY. Victor R. Santos 1,2 ; Raymund Pun2; Candi LaSarge 2; Norberto Garcia-Cairasco1; Steve Danzer2. 1. Department of

Physiology, Ribeirão Preto Medical School, Ribeirão Preto – SP, Brazil. 2. Department of Anesthesia, Cincinnati Childrens Hospital Medical Center, Cincinnati – OH, USA.

6. FINANTIAL SUPPORT

1) Grants for national students (12 selected students x 150 Euros)……. 2.100 Euros

2) Grants for local students (12 selected students x 50 Euros)…………… 550 Euros

3) Air tickets (faculties) .…..…………………………………………………... 200 Euros

4) Lunch and coffee breaks …………………………………………………... 400 Euros

5) Other expenses (transportation and photocopies)… …………………… 250 Euros

TOTAL… …………………………………………………………………… 3.500 Euros

7. SOME PICTURES OF THE WORKSHOP IN NEUROGENESIS

Openning ceremony with the presence of State University of Maringá Rector Dr Mauro Baeso and Health Science Directors.

Lecture Dr Rúbia Weffort de Oliveira: Neurogenesis: a complex multi-step process

Lecture Dr Alline C. Campos Behavioral implication of adult neurogenesis during stress responses

Lecture Dr Cilene Lino de Oliveira Environment influence on adult neurogenesis

Lecture Dr Lorena Varela Nallar Adult neurogenesis in Alzheimer’s disease

The audience: “Neurogenesis: basic aspects and implications on neuropsychiatric conditions”

Poster presentation Workshop: “Neurogenesis: basic aspects and implications

on neuropsychiatric conditions”

Seminars in the afternoon

Selected students receiving the IBROS’s grants

After the seminars