id3 polyclonal antibody · human id3 (np_002158.3). formulation: pbs with 0.02% sodium azide, 50%...

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ID3 Polyclonal Antibody Catalog Number: 164496 1 / 3 General Information Product name: ID3 Polyclonal Antibody Immunogen: Applications: WB, IHC, IF Specificity: Human, Mouse, Rat Antibody description: Polyclonal antibody to ID3 Properties Preparation: Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-119 of human ID3 (NP_002158.3). Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Storage: Store at -20℃. Avoid freeze / thaw cycles. Clonality: Polyclonal Ig Type:Rabbit IgG Applications WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 Validations Western blot - ID3 Polyclonal Antibody Western blot analysis of extracts of mouse liver, using ID3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Immunohistochemistry - ID3 Polyclonal Antibody Immunohistochemistry of paraffin-embedded rat brain using ID3 antibody at dilution of 1:200 (40x lens).

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Page 1: ID3 Polyclonal Antibody · human ID3 (NP_002158.3). Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Storage: Store at -20℃. Avoid freeze / thaw cycles. Clonality:

ID3 Polyclonal Antibody

Catalog Number: 164496

1 / 3

General Information

Product name:

ID3 Polyclonal Antibody

Immunogen:

Applications:

WB, IHC, IF

Specificity:

Human, Mouse, Rat

Antibody description:

Polyclonal antibody to ID3

Properties

Preparation:

Antigen: Recombinant fusion protein containing asequence corresponding to amino acids 1-119 ofhuman ID3 (NP_002158.3).

Formulation:

PBS with 0.02% sodium azide, 50% glycerol,pH7.3.

Storage:

Store at -20℃. Avoid freeze / thaw cycles.

Clonality:

Polyclonal

Ig Type:Rabbit IgG

Applications

WB 1:500 - 1:2000IHC 1:50 - 1:200IF 1:50 - 1:100

Validations

Western blot - ID3 Polyclonal Antibody

Western blot analysis of extracts of mouse liver,using ID3 antibody at 1:1000 dilution.Secondaryantibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug perlane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry - ID3 Polyclonal Antibody

Immunohistochemistry of paraffin-embedded ratbrain using ID3 antibody at dilution of 1:200 (40xlens).

Page 2: ID3 Polyclonal Antibody · human ID3 (NP_002158.3). Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Storage: Store at -20℃. Avoid freeze / thaw cycles. Clonality:

ID3 Polyclonal Antibody

Catalog Number: 164496

2 / 3

Immunohistochemistry - ID3 Polyclonal Antibody

Immunohistochemistry of paraffin-embeddedmouse kidney using ID3 antibody at dilution of1:200 (40x lens).

Immunohistochemistry - ID3 Polyclonal Antibody

Immunohistochemistry of paraffin-embeddedmouse pancreas using ID3 antibody at dilution of1:200 (40x lens).

Immunohistochemistry - ID3 Polyclonal Antibody

Immunohistochemistry of paraffin-embeddedmouse brain using ID3 antibody at dilution of1:200 (40x lens).

Immunohistochemistry - ID3 Polyclonal Antibody

Immunohistochemistry of paraffin-embeddedmouse spleen using ID3 antibody at dilution of1:200 (40x lens).

Immunohistochemistry - ID3 Polyclonal Antibody

Immunohistochemistry of paraffin-embeddedhuman lung cancer using ID3 antibody at dilutionof 1:200 (40x lens).

Page 3: ID3 Polyclonal Antibody · human ID3 (NP_002158.3). Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Storage: Store at -20℃. Avoid freeze / thaw cycles. Clonality:

ID3 Polyclonal Antibody

Catalog Number: 164496

3 / 3

Immunofluorescence - ID3 Polyclonal Antibody

Immunofluorescence analysis of HeLa cells usingID3 antibody .