improved rootstocks for imperial mandarin · 1. gayndah ‘imperial’ rootstock experiment 2. ctv...
TRANSCRIPT
![Page 1: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/1.jpg)
Improved rootstocks for Imperial mandarin
![Page 2: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/2.jpg)
Prior to 2000…… Troyer and Cleopatra
Benton for Imperial 2003, Sunstate Experiment
Barkley for Imperial 2017, Bakers Experiment
Industry impact: proven track record
![Page 3: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/3.jpg)
13-years-old
Troyer: Benching & tree decline Cleopatra: Phytophthora challenge
![Page 4: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/4.jpg)
Troyer 1960s Benton 2003 Barkley 2017
Joint field-day: 7th March 2017, Gayndah Organised by: QRAC, Citrus Australia Ltd Queensland Citrus Improvement Scheme Inc. Dept of Agriculture & Fisheries Queensland RSVP 3rd March (this Friday)
![Page 5: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/5.jpg)
“Growing to market requirements”
What’s that got do with
rootstocks?
![Page 6: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/6.jpg)
![Page 7: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/7.jpg)
![Page 8: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/8.jpg)
CT13004 Queensland Citrus Improvement Scheme: finding better rootstocks for Australia.
Funding: Voluntary Contribution project
![Page 9: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/9.jpg)
1. Gayndah ‘Imperial’ rootstock experiment
2. CTV replication and movement in advanced rootstocks
3. Emerald ‘Imperial’ rootstock experiment
4. Breeding new P.trifoliata hybrids with CTV resistance
5. The commercial value of “Extreme hybrids” as rootstocks
![Page 10: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/10.jpg)
Field Trials: Tegege, Sunstate, 1998: 10 rootstocks→Benton Wallaville, McClennan 2000: 28 rootstocks Gayndah, Bakers, 2004: 33 rootstocks→Barkley Emerald, Presslers, 2011: 66 rootstocks Gayndah, Shepherds, 2013: 245 rootstocks Wallaville, Spencer Ranch, 2016: 138 rootstocks Mundubbera/Gayndah, MHP, 2016/17: 14 rootstocks
Laboratory and Nursery Trials: Citrus Tristeza Virus inoculation and screening: >2,500 hybrids Phytophthora screening: >1,500 hybrids
Why use Imperial scions?.....good ‘canary’
![Page 11: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/11.jpg)
Rootstock objective: better fruit quality
![Page 12: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/12.jpg)
Rootstock objective: better fruit quality
But…
![Page 13: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/13.jpg)
Essential: tolerance to CTV resistance to phytophthora root and collar rot good horticultural performance Desirable: salt tolerance lime tolerance resistance to nematodes tolerance to waterlogging control of tree size Minor importance: tolerance to exocortis drought tolerance cold tolerance seedy and nucellar embryony Australian National Citrus Rootstock Screening Working Party, 1985
Rootstock requirements for Australian conditions
![Page 14: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/14.jpg)
Breeding for CTV resistance
Better method, faster results
>800 hybrids completely resistant to CTV, carrying the resistance gene from Poncirus.
New challenge…rootstock hybrids with 2 copies of the resistance gene → rapid field deployment.
Infected bud onto each hybrid
Hybrid bud onto infected rootstock
![Page 15: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/15.jpg)
Breeding for phytophthora resistance
Old Method
New Method Cheaper Faster Cleaner
![Page 16: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/16.jpg)
Breeding for salt tolerance
![Page 17: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/17.jpg)
Breeding for granulation resistance Use field trials to identify potential parents (with low granulation) Hybridise these parents Cull for CTV, phytophthora, salt Assess for granulation Unproven method
![Page 18: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/18.jpg)
Essential: tolerance to CTV resistance to phytophthora root and collar rot good horticultural performance Desirable: salt tolerance lime tolerance resistance to nematodes tolerance to waterlogging control of tree size Minor importance: tolerance to exocortis drought tolerance cold tolerance seedy and nucellar embryony Australian National Citrus Rootstock Screening Working Party, 1985
Rootstock requirements for Australian conditions
![Page 19: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/19.jpg)
Essential: tolerance to CTV resistance to phytophthora root and collar rot good horticultural performance HLB tolerance Desirable: salt tolerance lime tolerance resistance to nematodes tolerance to waterlogging control of tree size warm climate productivity Minor importance: tolerance to exocortis drought tolerance cold tolerance seedy and nucellar embryony gall wasp
Rootstock requirements for Australian conditions
![Page 20: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/20.jpg)
Breeding for new challenges Choose the right parents (e.g. pumelo, Eremocitrus, Microcitrus for HLB tolerance)
Maximise genetic diversity Build breeding populations that have the ‘right stuff’ and dump the rest
![Page 21: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/21.jpg)
e.g. K15 x 14Q055
• 556 hybrid seedlings
• 88% fail phytophthora screening
• 66 hybrids remaining
– 71% fail CTV screening
– 19 hybrids remaining
• ??% fail salt screening
• perhaps ~10 hybrids for field rootstock testing
~98% of hybrids dumped within 2 years of sowing, and prior to field planting. Minimum cost and effort.
![Page 22: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/22.jpg)
• Planted August 2011 • 65 different rootstocks, 891 data trees • Detailed assessments 2014, 2015, 2016
• Major impacts on canopy size,
productivity, quality
• Emergence of winners and losers
Emerald Imperial Rootstock Trial
![Page 23: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/23.jpg)
N639 X639 H639 Nelspruit Don’t use it!
![Page 24: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/24.jpg)
Wallaville Imperial Rootstock Trial
Planted November 2016 605 trees 138 rootstocks (screened for CTV and
phytophthora resistance prior to planting)
Genetic diversity Hybrids containing: Poncirus, Swinglea, Clymenia, Atalantia, Fortunella, Severinia, Citropsis, Hesperathusa, Citrus australasica, C.garrawayi, C.glauca, C.junos, C.wintersii, C.reticulata, C.maxima, C.aurantium.
The most genetically diverse rootstock experiment ever planted
![Page 25: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/25.jpg)
Thankyou
• Funding: DAF Qld, Hort Innovation, Qld Citrus Improvement Scheme
• Staff: Deb Gultzow, Toni Newman
• Field-trial hosts: Bakers, Benhams, Berthelsens, Emmertons, Presslers, Shepherds,
Spencer, Trotts.
• Professional input and supplies: Andrew Miles and other private
researchers/consultants. Helen Hofman, Graeme Sanderson, Nerida Donovan, Mark
Skewes, Matt Weinert, Yu Pei Tan and other Agency colleagues. Pat Barkley, Andrew
Harty and other industry professionals. John Owen-Turner and Committee Members
QCIS. Tim Herrman and the AusCitrus Board
• National/ International collaboration and germplasm: NSW DPI, Uni of California,
Riverside, CREA Italy, IAC New Caledonia, Jeju Uni South Korea, Uni of Florida Lake
Alfred, USDA Fort Pierce, USDA Riverside,
![Page 26: Improved rootstocks for Imperial mandarin · 1. Gayndah ‘Imperial’ rootstock experiment 2. CTV replication and movement in advanced rootstocks 3. Emerald ‘Imperial’ rootstock](https://reader030.vdocuments.net/reader030/viewer/2022040408/5eb9797ecaff2e7e2166c452/html5/thumbnails/26.jpg)
Thankyou
• Funding: DAF Qld, Hort Innovation, Qld Citrus Improvement Scheme
• Staff: Deb Gultzow, Toni Newman
• Field-trial hosts: Bakers, Benhams, Berthelsens, Emmertons, Presslers, Shepherds,
Spencer, Trotts.
• Professional input and supplies: Andrew Miles and other private
researchers/consultants. Helen Hofman, Graeme Sanderson, Nerida Donovan, Mark
Skewes, Matt Weinert, Yu Pei Tan and other Agency colleagues. Pat Barkley, Andrew
Harty and other industry professionals. John Owen-Turner and Committee Members
QCIS. Tim Herrman and the AusCitrus Board
• National/ International collaboration and germplasm: NSW DPI, Uni of California,
Riverside, CREA Italy, IAC New Caledonia, Jeju Uni South Korea, Uni of Florida Lake
Alfred, USDA Fort Pierce, USDA Riverside,
Australian citrus growers/nurseries/consultants: for wanting better rootstocks!