induced pluripotent stem cells and somatic cardiac regeneration— an exploratory bioinformatic...
DESCRIPTION
In the exploratory analysis, the functional pathway networks of nucleostemin and iPS interact via TP53 (tumor protein P53) and Fgf (fibroblast growth factor), which could be further investigated to provide clues for the future research of postnatal cardiac regeneration.TRANSCRIPT
Induced Pluripotent Stem Cells and Somatic Cardiac
Regeneration— An Exploratory Bioinformatic Analysis
Induced Pluripotent Stem Cells and Somatic Cardiac
Regeneration— An Exploratory Bioinformatic Analysis
Robert J. Chen, MD, MPH ([email protected])
*Yau-Hua Yu, DDS, DMsc
Dept. of Cardiac Surg., Cheng-Hsin Hosp. Heart Center; Graduate Inst. of Epidemiology, Nat’l Taiwan Univ.
*School of Dentistry, Nat’l Yang-Ming Univ.;
*Dept. of Dentistry and Dept. of Med. Research and Education, Taipei Veterans General Hosp., Taipei, Taiwan
Robert J. Chen, MD, MPH ([email protected])
*Yau-Hua Yu, DDS, DMsc
Dept. of Cardiac Surg., Cheng-Hsin Hosp. Heart Center; Graduate Inst. of Epidemiology, Nat’l Taiwan Univ.
*School of Dentistry, Nat’l Yang-Ming Univ.;
*Dept. of Dentistry and Dept. of Med. Research and Education, Taipei Veterans General Hosp., Taipei, Taiwan
Backgrounds: Heart FailureBackgrounds: Heart Failure
End-stage heart failure Current: Heart transplantation Pitfalls: donor, infection, rejection, … Alternatives: IABP, ECMO, VAD,…
Future Xeno-transplantation? Stem cells and regeneration!
End-stage heart failure Current: Heart transplantation Pitfalls: donor, infection, rejection, … Alternatives: IABP, ECMO, VAD,…
Future Xeno-transplantation? Stem cells and regeneration!
Background: Stem CellsBackground: Stem Cells
Embryonic stem cells: ethics? Adult stem cells
Existing stem cells: harvesting? Induced pluripotent stem (iPS) cells
Reprogrammed from somatic cells Re-differentiate and regenerate into a new heart
Embryonic stem cells: ethics? Adult stem cells
Existing stem cells: harvesting? Induced pluripotent stem (iPS) cells
Reprogrammed from somatic cells Re-differentiate and regenerate into a new heart
ObjectiveObjective
Induced pluripotent stem (iPS) cells SOX2-OCT4-NANOG complex (ectopically induced
transcription factors) RepSox + OCT4-NANOG (Harvard, 2009)
Nucleostemin (GNL3) Pivotal role in cardiac repair
Interplay of GNL3 in iPS cells? Bioinformatics exploration
Induced pluripotent stem (iPS) cells SOX2-OCT4-NANOG complex (ectopically induced
transcription factors) RepSox + OCT4-NANOG (Harvard, 2009)
Nucleostemin (GNL3) Pivotal role in cardiac repair
Interplay of GNL3 in iPS cells? Bioinformatics exploration
MethodsMethods NCBI online Entrez Gene and Pubmed Literature review To identify candidates molecules that
interact with the following molecules. Nucleostemin (GNL3) SOX2 OCT4 (POU5F1) NANOG
NCBI online Entrez Gene and Pubmed Literature review To identify candidates molecules that
interact with the following molecules. Nucleostemin (GNL3) SOX2 OCT4 (POU5F1) NANOG
MethodsMethods
Ingenuity Pathway Analysis (IPA) 7.5 ® Pathway network of the interactants with
GNL3 and SOX2-OCT4-NANOG Functional analysis
Patho-physiological processes Canonical pathways
Ingenuity Pathway Analysis (IPA) 7.5 ® Pathway network of the interactants with
GNL3 and SOX2-OCT4-NANOG Functional analysis
Patho-physiological processes Canonical pathways
Functional NetworkFunctional Network
Molecules related to cardiovascular system development and function: CDKN2A TP53 (tumor protein p53) ID3 NPM1 IL2
P-value=8.4e-4 ~ 9.9e-4 by right-tailed Fisher Exact Test
Molecules related to cardiovascular system development and function: CDKN2A TP53 (tumor protein p53) ID3 NPM1 IL2
P-value=8.4e-4 ~ 9.9e-4 by right-tailed Fisher Exact Test
Canonical PathwaysCanonical Pathways
PPP2R5A: cardiac beta-adrenergic signaling Fgf (fibroblast growth factor): human embryonic
stem cell pluripotency. Nucleostemin (GNL3) interacts with NANOG of
the iPS network via two molecules Fgf TP53.
PPP2R5A: cardiac beta-adrenergic signaling Fgf (fibroblast growth factor): human embryonic
stem cell pluripotency. Nucleostemin (GNL3) interacts with NANOG of
the iPS network via two molecules Fgf TP53.
ConclusionConclusion
In the exploratory analysis, the functional pathway networks of nucleostemin and iPS interact via: TP53 (tumor protein P53) Fgf (fibroblast growth factor)
They could be further investigated to provide clues for the future research of postnatal cardiac regeneration.
In the exploratory analysis, the functional pathway networks of nucleostemin and iPS interact via: TP53 (tumor protein P53) Fgf (fibroblast growth factor)
They could be further investigated to provide clues for the future research of postnatal cardiac regeneration.
