Infrastructure Development & Cultivation Practices

Day 1, Plenary Session II, 1130 Hrs-1300 Hrs, 17th Dec 2009, MSME DI,Guindy, Chennai

An autonomous society of Government of Tamil Nadu and Confederation of Indian Industry

Tamil Nadu Technology Development & Promotion Center

Day 1, Plenary Session II, 1130 Hrs-1300 Hrs, 17th Dec 2009, MSME DI,Guindy, Chennai

Presented By: D K Sridhar Babu

CULTIVATION

OFOF

Calocybe Indica

RESOURCES FOR CULTIVATION OF MILKY MUSHROOM

LAND -- Plain , Well Drained, LAND -- Plain , Well Drained,

Open Land

RAWMATERIAL AVAILABILITY -- Paddy Straw or Begase, Sorgam

LABOUR --- As per Need

INFRASTRUCTURE NEEDED

l CEMENT WATER TANK

l SHED – SPAWN RUNNING/BED INCUBATION

l MUSHROOM SHEDl MUSHROOM SHED

l GRADING & PACKING HALL

l RAW MATERIAL STORAGE ROOM

EQUIPMENTS REQUIRED

lPADDY STRAW CHOPPER M/C

lAUTOCLAVE

lLAMINAR FLOW CHAMBER

TROLLYSlTROLLYS

lBAND SEALING M/C

lWATER SPRAYER

lUTENSILS

MUSHROOM TERMINOLOGIESl Culture

Isolated Fungai l Mother Spawn

Culture Inoculated and Fungal Propagated over Sorgam .l Bed Spawn

Spawn Multiplication through Mother Spawn inoculation (First Generation Seeds)

l Bedsl BedsBed Spawn spreader layer by layer with paddy straw in Polythene

cover.l Formed Bed

Fungal formed beds during incubation in Bed Forming Rooml Casing Beds

Half of the formed bed with applied sterile soil.

NEED OF INFRASTUCTURE

l BED FORMING ROOM-- Formation of Fungus over the paddy straw -- Racks for multi tier.

lMUSHROOM SHED-- Dome Shape covered with Sylphalein

Sheet.-- Formed Beds placed for Growing

Mushroom

NEED OF EQUIPMENTS

l AUTOCLAVE

-- Sterilizing the Sorgam, Soil, Paddy Straw

l LAMINAR FLOW CAHMBER

--- Bed Spawn inoculation from Mother Spawn.--- Bed Spawn inoculation from Mother Spawn.

l PADDY CHOPPER M/C---- Chopping Paddy straw for size reduction

l Water Spray Gun---- Spraying water in Mushroom Shed

l Band Sealer Machine---- Sealing the Cover

Production

Flow Flow

Chart

Media Preparation

Innoculation Culture

Mother Spawn production20 days

Bed Spawn production

Bed Preparation20 days

Bed Incubation

Cutting the bed in to half

Soil Casing

Placing the Beds in Mushroom Bed20 days

Harvesting

Grading

Packing

HOW TO SET UP

MUSHROOM

FARM

SELECTION OF LAND

WATER SOURCE ARRANGEMENT

CONSTRUCTION OF SPAWN/BED RUNNING ROOM

DIGGING OF MUSHROOM SHED

CONSTRUCTION OF PROCESSING HALL

LAB ARRANGEMENTS

STORAGE ROOM

SELECTION OF LAND

lPLAIN LAND

lEASY DIGGABLE

lWELL DRAINABILITY

lOPEN LANDlOPEN LAND

WATER SOURCE ARRANGEMENT

lOPEN OR BOREWELL ARRANGEMENTS

CONSTRUCTION OF SPAWN/BED RUNNING ROOM

l Stone/Cement Pillar with Coconut Fronds Roof.l Stone/Cement Pillar with Palmyra Fronds.

Preferably Rat proof shedPreferably Rat proof shedlWith Convenient entrance and cross ventilation

windowsl Provide Rack system to accommodate more

Bedsl Sesbania grandiflora stems can be used for

making racks

Digging Of Pit for Mushroom Culture Room

l Excavate the soil the area proposed for the M.Culture Room

l Construct the pit walls with Hollow blocks from the bottomthe bottom

l Compact the soil for better & smooth flooring.

lMount Iron skeleton to hold the poly Sheet

lMount the poly sheet

lMake convenient steps and entrance with good cross ventilation

Lab ArrangementslFix up the Auto clave

lLaminar air flow chamber

lConvenient water supply

lElectricity provision for the lightinglElectricity provision for the lightingProcessing/Storage Rooml Open Area for paddy Straw Storage, Cement Building For other Raw Material storage & processing Hall

OPEN PADDY STRAW STORAGE

HOW TO PREPARE SPAWN ?Mother Spawn Preparation

Mother spawn prepared from isolated Fungal through inoculating in Sorgam base seed material.

Glass Bottles preferable for Mother Spawn Production

Bed Spawn Preparation

PP Cover used for Bed Spawn Preparation 7 ’’ X 10’’- 200 GmsSorgam used as base material for spawn

Mother Spawn (1 gm) inoculated in bed spawn cover in laminar flow chamberlaminar flow chamber

Mouth Sealed with Non Absorbent Cotton

Seed pockets kept for sterilization in Autoclave for 1 hr.

Sterilized seeds kept in seed running room for formation.

How to Cultivate

l Chop the paddy straw in the machine 1” longl Soak the paddy straw in water tank for 24 Hrsl Disinfect the paddy straw with 200 ml of Pharmalein for 100 lit of water and

Carbandazim 10 g/100 lit waterl Dry the paddy straw with optimum moisture about 60 -65%l Take the PP bag of 14”x24” (150 TO 200 GAUGE)l Tie the poly bag with Jute twine at the bottoml Tie the poly bag with Jute twine at the bottoml Make it inside outl Introduce the Sterile & withered paddy straw one layer 2-3”l Introduce the mushroom Spawns on to it (only on the edges)l Repeat the process up to the top.l Make sure that the paddy straw should be the last layerl Tie the Poly bag on the top with Jute/nylon twinel Make some holes (PIN PRICK) in the poly bags for respiration l Take them to the Spawn running/bed formation hut.

Spawn running/bed formation

l Keep the beds in racks closely(2-3’gap)l Make tiersl Spray water twice/thrice according to the temperature

l Spread some sand in the floor in summer months.l Spread some sand in the floor in summer months.l Keep some poison baits out side the Hut for controlling

the ratsl May maintain cats for effective control of rats.l Notice white icy colour developed within the bag within

20 days

SPAWN/BED RUNNING ROOM

FORMED BED

MUSHROOM CULTIVATION SHED

• Sheds constructed in Dome Shape

• Sheds has to be provided 4 ft from the ground and the Total height to be 8 ft

• Steel Angles/PVC Pipes used for Dome arrangements

• Hallow Block/Bricks used for side wall construction• Hallow Block/Bricks used for side wall construction

• Sylphalein sheet used as shed covering ( 90 gauge/ blue colour)

• 2000 Lux in the dome optimum for milky mushroom cultivation.

• Both Ends window id provided for ventilation.

SET UP MUSHROOM SHED

Casing the Beds

l Take out the formed beds from the Hutl Check the beds for other fungal coloniesl Cut the good beds in two halvesl Apply the sterilized soil for casingl Spray water on the cased soil l Place the beds in the mushroom culture roomsl Place the beds in the mushroom culture roomsl Sprinkle water in the cultivation rooml Ensure the wetness in the casing soill Check daily for any other fugal formation other than Milk

Mushrooml Notice the buds on the casing soil within 8 daysl Allow the mushroom to grow up to the optimum size

SOIL CASING

BUDDING MUSHROOM

Harvesting and cleaning

lUp root the matured mushroom from the bed

lClean the mushroom with dry cloth

lCut the bottom soiled area with knifelCut the bottom soiled area with knife

MUSHROOM AT HARVESTING STAGE

Grading & Packing

lGrading the Mushroom on size and Wt

lSorting the Mushroom according to the Quality.

lWeighing and packing the Mushroom in lWeighing and packing the Mushroom in LDPE pouch.

lHoles has to be provided in the pouch for ventilation.

lShelf Life 4 to 5 days

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An autonomous society of Government of Tamil Nadu and Confederation of Indian Industry

Tamil Nadu Technology Development & Promotion Center

TNTDPC is not responsible for the validity or accuracy of information and/or suggestions provided in the presentation and cannot be held responsible for any consequences arising from the usage of the same.