PATENTESTitleCATEGORAPublication numberPublication dateInventor(s)Applicant(s)International classificationCooperative Patent ClassificationApplication numberDate of applicationPriority number(s)Supplementary international search NPL citationAnti-microbial body care productUS6720006 (B2); US2002122832 (A1)9/5/02HANKE BERNHARD [DE]; GUGGENBICHLER PETER J [DE]HANKE BERNHARD, ; GUGGENBICHLER PETER JA01N59/16; A61C17/00; A61F13/15; A61K8/02; A61K8/04; A61K8/19; A61K8/31; A61K8/81; A61K8/85; A61K8/86; A61K8/87; A61K8/88; A61K8/89; A61K33/38; A61L15/18; A61L15/34; A61L15/46; A61Q11/00; A61Q15/00; A61Q17/00; A61Q19/00; A46D1/00; A61J17/00; A61K33/38A61Q17/005; A01N59/16; A46D1/006; A61F13/8405; A61K8/0208; A61K8/0241; A61K8/04; A61K8/19; A61K8/31; A61K8/8111; A61K8/8147; A61K8/8152; A61K8/85; A61K8/86; A61K8/87; A61K8/88; A61K8/89; A61K33/38; A61L15/18; A61L15/34; A61L15/46; A61Q11/00; A61Q15/00; A61Q19/00; B82Y5/00; A46D1/00; A61J17/00; A61K2800/413; A61L2300/104; A61L2300/404; A61L2300/624US20010017996200112142001EP19990111729 19990617; US20010017996 20011214; WO2000US15897 20000609Broad-spectrum antibacterial nano ointmentCN1480045 (A)3/10/04LIN YAN [CN]LIN YAN [CN]A01N59/16; A01N59/16CN2003130426200307212003CN2003130426 20030721ANTIBACTERIAL COMPOSITIONWO2012150890 (A1)11/8/12CARLSSON ANDERS [SE]; HERSLOEF BENGT [SE]; HOLMBAECK JAN [SE]LIPIDOR AB [SE]; CARLSSON ANDERS [SE]; HERSLOEF BENGT [SE]; HOLMBAECK JAN [SE]A61K47/24; A61K8/58; A61K33/38; A61K38/04; A61P31/02A61K8/34; A61K8/46; A61K8/553; A61Q17/005; A61K8/891; A61K8/19; A61K33/38; A61K9/7007; A61K2800/413WO2012SE0005620120420SE20110000341 20110502METHOD FOR PREPARING HYDROPHILLIC TEXTILES WITH ANTIMICROBIAL PROPERTIESRU2456995 (C1)7/27/12ZOLINA LJUDMILA IVANOVNA [RU]; BARANOVA OL GA NIKOLAEVNA [RU]; MISHAKOV VIKTOR JUR EVICH [RU]; BARANOV VALERIJ DMITRIEVICH [RU]TS NOVYKH T I BIZNESA AOZT [RU]A01N59/00; A61K33/38; A61L15/46; B82B3/00RU2011011690520110428RU20110116905 20110428FUNCTIONAL SOAP CONTAINING PEARL POWDER WITH SKIN SOOTHING AND STERILIZING ACTIVITIES AND SILVER NANOPARTICLES AND PREPARATION METHOD THEREOFKR20040085132 (A)10/7/04KIM HYEON YONG; LEE HYEON SOOKLEE HYEON SOOKC11D9/50; C11D9/50KR20040073470200409142004KR20040073470 20040914Method for preparing gold and silver nanoparticles with hydro-thermal methodCN102205421 (A)10/5/11YOUFU ZHOU; MAOCHUN HONG; FEILONG JIANG; LIAN CHENFUJIAN INST RES STR MATTER CASB22F9/24CN2010113679520100331CN20101136795 20100331PAIN RELIEF COMPOSITION COMPRISING PARAMAGNETIC SILVER NANOPARTICLESUS2010159016 (A1)6/24/10KIM YOUNG-NAM [KR]NANO PLASMA CT CO LTD [KR]A61K9/14; A61K33/38; A61P19/02A61K33/38; A61K33/00; A61K33/24; A61K45/06; B82Y5/00; B82Y30/00US2010071854020100305KR20060068357 20060721; US20050588135 20050401; US20070821796 20070626; US20100718540 20100305SILVER/POLYMER COLLOIDAL NANOCOMPOSITES AND A PROCESS FOR PREPARATION OF THE SAME, AND COSMETIC COMPOSITIONS CONTAINING THE SAMEWO2005077329 (A1)8/25/05KIM JIN WOONG [KR]; LEE JONG SUK [KR]; KIM SU JIN [KR]; KIM YONG JIN [KR]; KIM JUNOH [KR]; HAN SANG HOON [KR]; CHANG IH SEOP [KR]AMOREPACIFIC CORP [KR]; KIM JIN WOONG [KR]; LEE JONG SUK [KR]; KIM SU JIN [KR]; KIM YONG JIN [KR]; KIM JUNOH [KR]; HAN SANG HOON [KR]; CHANG IH SEOP [KR]A01N59/16; A61K8/04; A61K8/19; A61K8/72; A61K8/81; A61Q19/00; A61K7/48B82Y5/00; A01N59/16; A61K8/0279; A61K8/19; A61K8/8152; A61Q19/00; A61K2800/413; A61K2800/524; A61K2800/621WO2005KR00371200502072005KR20040008909 20040211Santiary protective pad of fragrance agentsCN2774443 (Y)4/26/06CHENG ZHIXIN [CN]CHENG ZHIXIN [CN]A61F13/15; A61L15/42CN2005210940U200503292005CN2005210940U 20050329PREPARATION METHOD FOR FUNCTIONAL FABRIC HAVING ANTIBIOTIC AND MOISTURE ABSORPTION PROPERTIES USING SLIVER NANOPATICLES INCORPORATED MESOPOROUS SILLICAKR20100077896 (A); KR101099791 (B1)7/8/10CHANG JEONG HO [KR]; KANG SANG HWA [KR]; LEE HYE SUN [KR]KOREA INST CERAMIC ENG & TECH [KR]D06M11/42; D06M11/77; D06M15/564KR2008013597120081229KR20080135971 20081229DETERGENT COMPOSITION COMPRISING PARAMAGNETIC SILVER NANOPARTICLESKR20070060229 (A); KR100731556 (B1)6/13/07KIM YOUNG NAM [KR]KIM YOUNG NAM [KR]C11D3/02KR20050119340200512082005KR20050119340 20051208CREAM FOR MILKING ''DENNICA''RU2367425 (C1)9/20/09KREJTSBERG GEORGIJ NIKOLAEVICH [RU]; GOLIKOV IGOR VITAL EVICH [RU]; ZAVOJSTYJ IVAN VITAL EVICH [RU]; TANIFA VIKTOR VASIL EVICH [RU]OOO NPOB LIKOM [RU]A61K31/19; A61K33/38; A61K36/28; A61K36/886; A61P17/02RU2008011230020080402RU20080112300 20080402COMPOSITION FOR FUNCTIONAL SOAP COMPRISING GOLD OR SILVER NANOPARTICLE AND EXTRACTION POLYMER OF BLACK NIGHTSHADE AS EFFECTIVE INGREDIENTS, AND PRODUCTION METHOD THEREOFKR100847478 (B1)7/21/08LEE SU JEONG [KR]; KYUM MYOUNG CHUL [KR]; LEE IL SOO [KR]ABC NANOTECH LTD [KR]C11D9/06; C11D9/04; C11D9/60KR20070036418200704132007KR20070036418 20070413SILK MASK SHEET CONTAINING THE SILVER NANOPARTICLESKR20090105379 (A)10/7/09SIM YOUNG HWA [KR]; CHAE BYUNG SUK [KR]; KIM DOO HO [KR]; CHUNG BO MOOK [KR]KPM TECH CO LTD [KR]A61K8/19; A61Q19/00KR20080030805200804022008KR20080030805 20080402NANOSOAP CONTAINING SILVER NANOPARTICLESUS2010056485 (A1)3/4/10PARK SEUNG BUM [KR]SNU R&DB FOUNDATIONA61K31/661; A61K31/28; A61K31/555; A61P31/04; A61P31/10; A61P31/12A61K31/28; A61K31/555; A61K31/661; A61K33/38US20080200817200808282008US20080200817 20080828PROPHYLACTIC AGENT FOR SKIN WITH ANTITUBERCULOUS EFFECTRU2409367 (C2); RU2009114003 (A)10/20/10KREJTSBERG GEORGIJ NIKOLAEVICH, ; GOLIKOV IGOR' VITAL'EVICH, ; ZAVOJSTYJ IVAN VITAL'EVICH, ; USTAVSHCHIKOV OLEG BORISOVICH, ; KREJTSBERG OL'GA GEORGIEVNAOBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOST'JU "NAUCHNO-PROIZVODSTVENNOE OB"EDINENIE "LIKOM"A61K9/06; A61K31/7036; A61K33/38; A61K47/30; A61P17/00; A61P31/06; B82B1/00RU20090114003200904132009RU20090114003 20090413MEDICATION FOR TREATMENT AND PREVENTION OF FUNGAL SKIN DISEASESRU2428184 (C1); RU2009145462 (A)6/20/11KREJTSBERG GEORGIJ NIKOLAEVICH, ; ZAVOJSTYJ IVAN VITAL'EVICH, ; GRACHEVA IRINA EVGEN'EVNA, ; KREJTSBERG OL'GA GEORGIEVNAOBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOST'JU "NAUCHNO-PROIZVODSTVENNOE OB"EDINENIE "LIKOM"A61K31/01; B82B3/00RU20090145462200912092009RU20090145462 20091209Silver/Water, Silver Gels and Silver-Based CompositionsNZ590025 (A)6/29/12HOLLADAY ROBERT; MOELLER WILLIAM; MEHTA DILIP; BROOKS JULIANA HROBERT HOLLADAY; WILLIAM MOELLER; DILIP MEHTA; JULIANA H J BROOKS; RUSTUM ROY; MARK MORTENSONG01N33/53; A61K33/38A61K33/38; A01N59/16; A61K31/28; A61K31/65; A61K33/24; A61K33/30; A61K33/34; A61K45/06NZ20050590025200512302005US20050641521P 20050105; US20050697079P 20050707; US20050702494P 20050726PROCESS FOR OBTAINING WOOL-LINED SKIN WITH BIOCIDAL PROPERTIES AND SKIN OBTAINED THEREBYRO127655 (A2)7/30/12GAIDAU CARMEN CORNELIA [RO]; MARTINESCU TAMARA NICOLETA [RO]; CIOBANU CONSTANTIN [RO]; IGNAT MAURUSA ELENA [RO]INST NATIONAL DE CERCETARE DEZVOLTARE PENTRU TEXTILE SI PIELARIE SUCURSALA INST [RO]C14C13/00RO20100001077201011092010RO20100001077 20101109Silver nanoparticles, preparation method thereof and nano silver dressingCN102764448 (A)11/7/12LIBO ZHANGLIBO ZHANGA61L15/44CN20121225060201207022012CN20121225060 20120702DEODORIZING AND ANTIBACTERIAL WET TISSUE FOR FOOT, AND MANUFACTURING METHOD THEREOFKR20130037311 (A)4/16/13WON MOON SIK [KR]WON MOON SIK [KR]D21H21/14; A47K10/16; D21H17/20; D21H19/10KR20110101651201110062011KR20110101651 20111006

Patentes del temaTitleCATEGORAPublication numberPublication dateInventor(s)Applicant(s)International classificationCooperative Patent ClassificationApplication numberDate of applicationAo de aplicacinPriority number(s)Cdigo pasPasSilver nanoparticles, preparation method thereof and nano silver dressingANTIBACTERIALCN102764448 (A)11/7/12LIBO ZHANGLIBO ZHANGA61L15/44CN20121225060201207022012CN20121225060 20120702CNRepublica de ChinaDEODORIZING AND ANTIBACTERIAL WET TISSUE FOR FOOT, AND MANUFACTURING METHOD THEREOFANTIBACTERIALKR20130037311 (A)4/16/13WON MOON SIK [KR]WON MOON SIK [KR]D21H21/14; A47K10/16; D21H17/20; D21H19/10KR20110101651201110062011KR20110101651 20111006KRKorea del SurPROCESS FOR OBTAINING WOOL-LINED SKIN WITH BIOCIDAL PROPERTIES AND SKIN OBTAINED THEREBYRECUPERACION DE LA PIELRO127655 (A2)7/30/12GAIDAU CARMEN CORNELIA [RO]; MARTINESCU TAMARA NICOLETA [RO]; CIOBANU CONSTANTIN [RO]; IGNAT MAURUSA ELENA [RO]INST NATIONAL DE CERCETARE DEZVOLTARE PENTRU TEXTILE SI PIELARIE SUCURSALA INST [RO]C14C13/00RO20100001077201011092010RO20100001077 20101109RORumaniaMEDICATION FOR TREATMENT AND PREVENTION OF FUNGAL SKIN DISEASESRECUPERACION DE LA PIELRU2428184 (C1); RU2009145462 (A)6/20/11KREJTSBERG GEORGIJ NIKOLAEVICH, ; ZAVOJSTYJ IVAN VITAL'EVICH, ; GRACHEVA IRINA EVGEN'EVNA, ; KREJTSBERG OL'GA GEORGIEVNAOBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOST'JU "NAUCHNO-PROIZVODSTVENNOE OB"EDINENIE "LIKOM"A61K31/01; B82B3/00RU20090145462200912092009RU20090145462 20091209RUFederacion de RusiaPROPHYLACTIC AGENT FOR SKIN WITH ANTITUBERCULOUS EFFECTANTIBACTERIALRU2409367 (C2); RU2009114003 (A)10/20/10KREJTSBERG GEORGIJ NIKOLAEVICH, ; GOLIKOV IGOR' VITAL'EVICH, ; ZAVOJSTYJ IVAN VITAL'EVICH, ; USTAVSHCHIKOV OLEG BORISOVICH, ; KREJTSBERG OL'GA GEORGIEVNAOBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOST'JU "NAUCHNO-PROIZVODSTVENNOE OB"EDINENIE "LIKOM"A61K9/06; A61K31/7036; A61K33/38; A61K47/30; A61P17/00; A61P31/06; B82B1/00RU20090114003200904132009RU20090114003 20090413RUFederacion de RusiaNANOSOAP CONTAINING SILVER NANOPARTICLESRECUPERACION DE LA PIELUS2010056485 (A1)3/4/10PARK SEUNG BUM [KR]SNU R&DB FOUNDATIONA61K31/661; A61K31/28; A61K31/555; A61P31/04; A61P31/10; A61P31/12A61K31/28; A61K31/555; A61K31/661; A61K33/38US20080200817200808282008US20080200817 20080828USEstados UnidosSILK MASK SHEET CONTAINING THE SILVER NANOPARTICLESANTIBACTERIALKR20090105379 (A)10/7/09SIM YOUNG HWA [KR]; CHAE BYUNG SUK [KR]; KIM DOO HO [KR]; CHUNG BO MOOK [KR]KPM TECH CO LTD [KR]A61K8/19; A61Q19/00KR20080030805200804022008KR20080030805 20080402KRKorea del SurCOMPOSITION FOR FUNCTIONAL SOAP COMPRISING GOLD OR SILVER NANOPARTICLE AND EXTRACTION POLYMER OF BLACK NIGHTSHADE AS EFFECTIVE INGREDIENTS, AND PRODUCTION METHOD THEREOFANTIBACTERIALKR100847478 (B1)7/21/08LEE SU JEONG [KR]; KYUM MYOUNG CHUL [KR]; LEE IL SOO [KR]ABC NANOTECH LTD [KR]C11D9/06; C11D9/04; C11D9/60KR20070036418200704132007KR20070036418 20070413KRKorea del SurSilver/Water, Silver Gels and Silver-Based CompositionsANTIBACTERIALNZ590025 (A)6/29/12HOLLADAY ROBERT; MOELLER WILLIAM; MEHTA DILIP; BROOKS JULIANA HROBERT HOLLADAY; WILLIAM MOELLER; DILIP MEHTA; JULIANA H J BROOKS; RUSTUM ROY; MARK MORTENSONG01N33/53; A61K33/38A61K33/38; A01N59/16; A61K31/28; A61K31/65; A61K33/24; A61K33/30; A61K33/34; A61K45/06NZ20050590025200512302005US20050641521P 20050105; US20050697079P 20050707; US20050702494P 20050726USEstados UnidosDETERGENT COMPOSITION COMPRISING PARAMAGNETIC SILVER NANOPARTICLESANTIBACTERIALKR20070060229 (A); KR100731556 (B1)6/13/07KIM YOUNG NAM [KR]KIM YOUNG NAM [KR]C11D3/02KR20050119340200512082005KR20050119340 20051208KRKorea del SurSantiary protective pad of fragrance agentsANTIBACTERIALCN2774443 (Y)4/26/06CHENG ZHIXIN [CN]CHENG ZHIXIN [CN]A61F13/15; A61L15/42CN2005210940U200503292005CN2005210940U 20050329CNRepublica de ChinaSILVER/POLYMER COLLOIDAL NANOCOMPOSITES AND A PROCESS FOR PREPARATION OF THE SAME, AND COSMETIC COMPOSITIONS CONTAINING THE SAMEPRESERVACIN DE PTOS COSMETICOSWO2005077329 (A1)8/25/05KIM JIN WOONG [KR]; LEE JONG SUK [KR]; KIM SU JIN [KR]; KIM YONG JIN [KR]; KIM JUNOH [KR]; HAN SANG HOON [KR]; CHANG IH SEOP [KR]AMOREPACIFIC CORP [KR]; KIM JIN WOONG [KR]; LEE JONG SUK [KR]; KIM SU JIN [KR]; KIM YONG JIN [KR]; KIM JUNOH [KR]; HAN SANG HOON [KR]; CHANG IH SEOP [KR]A01N59/16; A61K8/04; A61K8/19; A61K8/72; A61K8/81; A61Q19/00; A61K7/48B82Y5/00; A01N59/16; A61K8/0279; A61K8/19; A61K8/8152; A61Q19/00; A61K2800/413; A61K2800/524; A61K2800/621WO2005KR00371200502072005KR20040008909 20040211KRKorea del SurFUNCTIONAL SOAP CONTAINING PEARL POWDER WITH SKIN SOOTHING AND STERILIZING ACTIVITIES AND SILVER NANOPARTICLES AND PREPARATION METHOD THEREOFRECUPERACION DE LA PIELKR20040085132 (A)10/7/04KIM HYEON YONG; LEE HYEON SOOKLEE HYEON SOOKC11D9/50; C11D9/50KR20040073470200409142004KR20040073470 20040914KRKorea del SurBroad-spectrum antibacterial nano ointmentANTIBACTERIALCN1480045 (A)3/10/04LIN YAN [CN]LIN YAN [CN]A01N59/16; A01N59/16CN2003130426200307212003CN2003130426 20030721CNRepublica de ChinaAnti-microbial body care productANTIBACTERIALUS6720006 (B2); US2002122832 (A1)9/5/02HANKE BERNHARD [DE]; GUGGENBICHLER PETER J [DE]HANKE BERNHARD, ; GUGGENBICHLER PETER JA01N59/16; A61C17/00; A61F13/15; A61K8/02; A61K8/04; A61K8/19; A61K8/31; A61K8/81; A61K8/85; A61K8/86; A61K8/87; A61K8/88; A61K8/89; A61K33/38; A61L15/18; A61L15/34; A61L15/46; A61Q11/00; A61Q15/00; A61Q17/00; A61Q19/00; A46D1/00; A61J17/00; A61K33/38A61Q17/005; A01N59/16; A46D1/006; A61F13/8405; A61K8/0208; A61K8/0241; A61K8/04; A61K8/19; A61K8/31; A61K8/8111; A61K8/8147; A61K8/8152; A61K8/85; A61K8/86; A61K8/87; A61K8/88; A61K8/89; A61K33/38; A61L15/18; A61L15/34; A61L15/46; A61Q11/00; A61Q15/00; A61Q19/00; B82Y5/00; A46D1/00; A61J17/00; A61K2800/413; A61L2300/104; A61L2300/404; A61L2300/624US20010017996200112142001EP19990111729 19990617; US20010017996 20011214; WO2000US15897 20000609EPPatente de Europa

La patente ms recienteLa patente ms antiguaANTIBACTERIALRECUPERACION DE LA PIELPRESERVACIN DE PTOS COSMETICOS

Evolucin de patentes (aos)Etiquetas de filaCuenta de Ao de aplicacin20121201112010120092200822007120054200412003120011Total general15

Categorias-Diagrama de burbujasCuenta de CATEGORAEtiquetas de columnaEtiquetas de fila2001200320042005200720082009201020112012Total generalANTIBACTERIAL1131111110PRESERVACIN DE PTOS COSMETICOS11RECUPERACION DE LA PIEL11114Total general111412211115AosCategora2001200320042005200720082009201020112012ANTIBACTERIAL113111111111111111PRESERVACIN DE PTOS COSMETICOS12222222222RECUPERACION DE LA PIEL11113333333333

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Tema patentes

Silver nanoparticles, preparation method thereof and nano silver dressingparches externos nano o vendajes mdicos tienen una funcin nica o componentes complejos, que facilitan la cuera de los daos piel del usuario por envenenamiento y alergia a medicamentos.

DEODORIZING AND ANTIBACTERIAL WET TISSUE FOR FOOT, AND MANUFACTURING METHOD THEREOFUna toallita hmeda para los pies con el fin de eliminar de olores desagradables y con funciones antibacterianas.

MEDICATION FOR TREATMENT AND PREVENTION OF FUNGAL SKIN DISEASESMedicacin en forma de ungento para el tratamiento y la prevencin de las enfermedades fngicas de la piel.

PUBLICACIONESAuthorsTitleYearSource titleVolumeIssueArt. No.Page startPage endPage countCited byLinkDocument TypeSourceAuthors with affiliationsAbstract[No author name available]Materials Inspired by Biology2003Materials Research Society Symposium - Proceedings774http://www.scopus.com/inward/record.url?eid=2-s2.0-0345358721&partnerID=40&md5=5929ecebc8fb62fefc4017dcf89a1ce6Conference ReviewScopusThe proceedings contain 30 papers from the conference on Materials Inspired by Biology. The topics discussed include: carbon nanofiber surface roughness increases osteoblast adhesion; cytocompatibility of carbon nanofiber materials for neural applications; enhanced osteoblast adhesion on a novel hydroxyapatite coating; amelogenin induces biomimetic mineralization at specific pH; sea urchin mineralized tissue; intrafibrillar mineralization of collagen using a liquid-phase mineral precursor; and biosynthesis of silver nanoparticles.Abdel-Fattah, S.H., El-Khatib, E.M., Kantouch, A.M., El-Zawawi, I.K.Antimicrobial finishing of wool fabrics2010Energy Education Science and Technology Part A: Energy Science and Research2421371495http://www.scopus.com/inward/record.url?eid=2-s2.0-84861958648&partnerID=40&md5=a762ee752e82c021052da9eba5453b9aArticleScopusAbdel-Fattah, S.H., Textile Research Division, Dokki, Cairo, Egypt; El-Khatib, E.M., Textile Research Division, Dokki, Cairo, Egypt; Kantouch, A.M., Textile Research Division, Dokki, Cairo, Egypt; El-Zawawi, I.K., Solid State Physics Department National Research Centre, Dokki, Cairo, EgyptWool is a suitable medium for growing bacteria and fungi under favorable temperature and humidity conditions resulting in wool degradation and skin irritation or infections. Several investigations have been applied to impart antimicrobial finish of wool fabrics based on organic chemicals, but they are not environmental friendly. The present investigation aims to study the influence of treatment with metallic ions as well as silver nanoparticles, by nanolayer condensation, on wool fabrics, for improvement of its properties. Wool treated with the aforementioned methods show antimicrobial activity, UV-protection properties and improvement in conductivity and crease recovery. The changes in surface morphologies were observed. Sila Science.Agarwal, A., Guthrie, K.M., Czuprynski, C.J., Schurr, M.J., McAnulty, J.F., Murphy, C.J., Abbott, N.L.Polymeric multilayers that contain silver nanoparticles can be stamped onto biological tissues to provide antibacterial activity2011Advanced Functional Materials2110186318739http://www.scopus.com/inward/record.url?eid=2-s2.0-79956101438&partnerID=40&md5=926d5671730208168b48653832008a2fArticleScopusAgarwal, A., Department of Chemical and Biological Engineering, University of Wisconsin, 1415 Engineering Drive, Madison, WI 53706, United States; Guthrie, K.M., Department of Surgery, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Dr, Madison, WI 53706, United States; Czuprynski, C.J., Department of Pathobiology, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Dr, Madison, WI 53706, United States; Schurr, M.J., Department of Surgery, School of Medicine, University of Wisconsin-Madison, 600 Highland Ave, Madison, WI 53706, United States; McAnulty, J.F., Department of Surgery, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Dr, Madison, WI 53706, United States; Murphy, C.J., Department of Ophthalmology and Vision Sciences, School of Medicine, University of California-Davis, Davis, CA 95616, United States; Abbott, N.L., Department of Chemical and Biological Engineering, University of Wisconsin, 1415 Engineering Drive, Madison, WI 53706, United StatesThe design of polyelectrolyte multilayers (PEMs) that can be prefabricated on an elastomeric stamp and mechanically transferred onto biomedically-relevant soft materials, including medical-grade silicone elastomers (E'450-1500 kPa; E'-elastic modulus) and the dermis of cadaver skin (E'200-600 kPa), is reported. Whereas initial attempts to stamp PEMs formed from poly(allylamine hydrochloride) and poly(acrylic acid) resulted in minimal transfer onto soft materials, we report that integration of micrometer-sized beads into the PEMs (thicknesses of 6-160 nm) led to their quantitative transfer within 30 seconds of contact at a pressure of 196 kPa. To demonstrate the utility of this approach, PEMs were impregnated with a range of loadings of silver-nanoparticles and stamped onto the dermis of human cadaver skin (a wound-simulant) that was subsequently incubated with bacterial cultures. Skin dermis stamped with PEMs that released 0.25 0.01 g cm -2 of silver ions caused a 6 log 10 reduction in colony forming units of Staphylococcus epidermidis and Pseudomonas aeruginosa within 12 h. Significantly, this level of silver release is below that which is cytotoxic to NIH 3T3 mouse fibroblast cells. Overall, this study describes a general and facile approach for the functionalization of biomaterial surfaces without subjecting them to potentially deleterious processing conditions. Copyright 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Ahamed, M., AlSalhi, M.S., Siddiqui, M.K.J.Silver nanoparticle applications and human health2010Clinica Chimica Acta41123-2418411848119http://www.scopus.com/inward/record.url?eid=2-s2.0-77957753618&partnerID=40&md5=8f029855f5f202f064414c56babee823ReviewScopusAhamed, M., King Abdullah Institute for Nanotechnology, King Saud University, Riyadh-11451, Saudi Arabia; AlSalhi, M.S., King Abdullah Institute for Nanotechnology, King Saud University, Riyadh-11451, Saudi Arabia; Siddiqui, M.K.J., Council of Science and Technology-Uttar Pradesh, Lucknow-227017, IndiaNanotechnology is rapidly growing with nanoparticles produced and utilized in a wide range of commercial products throughout the world. For example, silver nanoparticles (Ag NP) are used in electronics, bio-sensing, clothing, food industry, paints, sunscreens, cosmetics and medical devices. These broad applications, however, increase human exposure and thus the potential risk related to their short- and long-term toxicity. A large number of in vitro studies indicate that Ag NPs are toxic to the mammalian cells derived from skin, liver, lung, brain, vascular system and reproductive organs. Interestingly, some studies have shown that this particle has the potential to induce genes associated with cell cycle progression, DNA damage and apoptosis in human cells at non-cytotoxic doses. Furthermore, in vivo bio-distribution and toxicity studies in rats and mice have demonstrated that Ag NP administered by inhalation, ingestion or intra-peritoneal injection were subsequently detected in blood and caused toxicity in several organs including brain. Moreover, Ag NP exerted developmental and structural malformations in non-mammalian model organisms typically used to elucidate human disease and developmental abnormalities. The mechanisms for Ag NP induced toxicity include the effects of this particle on cell membranes, mitochondria and genetic material. This paper summarizes and critically assesses the current studies focusing on adverse effects of Ag NPs on human health. 2010 Elsevier B.V.Ahmad, M.B., Shameli, K., Darroudi, M., Yunus, W.M.Z.W., Ibrahim, N.A.Synthesis and characterization of silver/clay nanocomposites by chemical reduction method2009American Journal of Applied Sciences6111909191413http://www.scopus.com/inward/record.url?eid=2-s2.0-72449143265&partnerID=40&md5=ab9a35edaef200974e18e95b9fc16266ArticleScopusAhmad, M.B., Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Shameli, K., Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Darroudi, M., Advanced Materials and Nanotechnology Laboratory, Institute of Advanced Technology, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Yunus, W.M.Z.W., Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Ibrahim, N.A., Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaProblem statement: Silver Nanoparticles (Ag-NPs) have been synthesized by using chemical reduction method into the interlayer space of a Montmorillonite (MMT) as a solid support which is used to antibacterial application and polymer nanocomposites for fabrication of medical devices. Approach: AgNO3 and NaBH4 were used as a silver precursor and reducing agent, respectively. The properties of Ag/MMT nanocomposites were studied as a function of the AgNO3 concentration. The crystalline structure, d-spacing of interlayer of MMT, the size distributions and surface plasmon resonance of synthesized Ag-NPs were characterized using Powder X-Ray Diffraction (PXRD), Transmission Electron Microscopy (TEM) and UV-vis spectroscopy. Results: The results obtained from UV-vis spectroscopy of synthesized Ag-NPs showed that the intensity of the maximum wavelength of the plasmon peaks were increased with the increasing in the AgNO3 concentration. The obtained information from UV-vis spectra of Ag-NPs was in an excellent agreement with the obtained microstructures studies performed by Transmission Electron Microscopy (TEM) and their size distributions. The prepared Ag/MMT nanocomposites are very stable over a long period of time in aqueous solution. Conclusion: The synthesized Ag/MMT nanocomposites are very stable in aqueous solution over a long period of time without any sign of precipitation. Silver nanoparticles in MMT suspension could be suitable to use antibacterial applications, since MMT is viewed as ecologically and environmentally inert material and used for biological application such as cosmetics and pharmaceutical usage. 2009 Science Publications.Ahmad, M.B., Shameli, K., Yunus, W.M.Z.W., Ibrahim, N.A., Darroudi, M.Synthesis and characterization of Silver/Clay/Starch bionanocomposites by green method2010Australian Journal of Basic and Applied Sciences472158216512http://www.scopus.com/inward/record.url?eid=2-s2.0-78649376662&partnerID=40&md5=c9190775e846ab38be0df913edfab2b8ArticleScopusAhmad, M.B., Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia; Shameli, K., Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia; Yunus, W.M.Z.W., Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia; Ibrahim, N.A., Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia; Darroudi, M., Advanced Materials and Nanotechnology Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, MalaysiaIn this study, we report an effective process for preparing silver nanoparticles (Ag NPs) by using Green reduction method of AgNO3 in interlamellar space of Montmorillonite/Starch Bionanocomposites (MMT/Stc BNCs) suspension with moderate temperature. In here MMT, Starch, -D-glucose and AgNO3 were used as a solid support, stabilizer, green reducing agent and silver precursor, respectively. Bionanocomposites material based on MMT, starch and silver nanoparticles (Ag/MMT/Stc BNCs) were prepared by adding starch and silver nitrate respectively into montmorillonite (MMT) dispersions in double distill water solution. The crystalline structure, d-spacing of interlayer of MMT, the size distributions, surface Plasmon resonance and functional groups of synthesized Ag NPs in the MMT/Stc BNCs were characterized using Powder X-Ray Diffraction (PXRD), Transmission Electron Microscopy (TEM), UV-visible spectroscopy and Fourier Transform Infrared Spectroscopy (FT-IR). The results obtained from TEM showed that the Ag NPs prepared in the extra surface of MMT layers have larger than Ag NPs intercalated between MMT layers, the particle size of nanoparticles synthesized by this processes were from 9 to 39 nm. Powder X-Ray Diffraction analysis showed that the synthesized Ag NPs crystallized in face centered cubic (fcc) symmetry. With gentle heating, this system is a mild, renewable, inexpensive, and nontoxic reducing agent. The synthesized bionanocomposites are very stable in aqueous solution over a long period of time (i.e., 3 months) without any sign of precipitation. Silver nanoparticles in MMT/Stc suspension could be suitable to use various medical applications. Since MMT is viewed as ecologically and environmentally inert material and used for biological application such as cosmetics and pharmaceutical usage. 2010, INSInet Publication.Akhavan, S., Gungor, K., Mutlugun, E., Demir, H.V.Plasmonic light-sensitive skins of nanocrystal monolayers2013Nanotechnology2415155201http://www.scopus.com/inward/record.url?eid=2-s2.0-84875679976&partnerID=40&md5=5952e6fe9b38103b5b7a645baad8146fArticleScopusAkhavan, S., UNAM-Institute of Materials Science and Nanotechnology, Department of Electrical and Electronics Engineering, Bilkent University, 06800 Ankara, Turkey; Gungor, K., UNAM-Institute of Materials Science and Nanotechnology, Department of Electrical and Electronics Engineering, Bilkent University, 06800 Ankara, Turkey; Mutlugun, E., UNAM-Institute of Materials Science and Nanotechnology, Department of Electrical and Electronics Engineering, Bilkent University, 06800 Ankara, Turkey, School of Electrical and Electronic Engineering, School of Physical and Mathematical Sciences, Nanyang Technological University, 639798 Singapore, Singapore; Demir, H.V., UNAM-Institute of Materials Science and Nanotechnology, Department of Electrical and Electronics Engineering, Bilkent University, 06800 Ankara, Turkey, School of Electrical and Electronic Engineering, School of Physical and Mathematical Sciences, Nanyang Technological University, 639798 Singapore, SingaporeWe report plasmonically coupled light-sensitive skins of nanocrystal monolayers that exhibit sensitivity enhancement and spectral range extension with plasmonic nanostructures embedded in their photosensitive nanocrystal platforms. The deposited plasmonic silver nanoparticles of the device increase the optical absorption of a CdTe nanocrystal monolayer incorporated in the device. Controlled separation of these metallic nanoparticles in the vicinity of semiconductor nanocrystals enables optimization of the photovoltage buildup in the proposed nanostructure platform. The enhancement factor was found to depend on the excitation wavelength. We observed broadband sensitivity improvement (across 400-650 nm), with a 2.6-fold enhancement factor around the localized plasmon resonance peak. The simulation results were found to agree well with the experimental data. Such plasmonically enhanced nanocrystal skins hold great promise for large-area UV/visible sensing applications. 2013 IOP Publishing Ltd.Amina, M., Amna, T., Hassan, M.S., Ibrahim, T.A., Khil, M.-S.Facile single mode electrospinning way for fabrication of natural product based silver decorated polyurethane nanofibrous membranes: Prospective medicated bandages2013Colloids and Surfaces A: Physicochemical and Engineering Aspects425115121http://www.scopus.com/inward/record.url?eid=2-s2.0-84875610697&partnerID=40&md5=ffd3f054e48dc54697695a9ead7167bdArticleScopusAmina, M., Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia; Amna, T., Department of Animal Science and Biotechnology, Chonbuk National University, Jeonju 561-756, South Korea; Hassan, M.S., Department of Organic Materials and Fiber Engineering, Chonbuk National University, Jeonju 561-756, South Korea; Ibrahim, T.A., Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia, Department of pharmacognosy, Faculty of pharmacy, Cairo University, Egypt; Khil, M.-S., Department of Organic Materials and Fiber Engineering, Chonbuk National University, Jeonju 561-756, South KoreaIn this study we have introduced for the first time a new class of bionanocomposite scaffold composed of virgin olive oil/poly(urethane) blend system decorated with silver nanoparticles via single mode electrospinning approach. Olive oil is a natural material that contains vitamin E, antioxidants and phenol that affect cytokine production by skin cells when applied topically and help in recovery process when skin damage occurs. Herein, we standardized optimal concentration (5%) of olive oil to get spinable solution for fabrication of oil blended nanofiberous membranes. The fabricated bionanocomposite membranes were characterized through scanning electron microscopy, energy dispersive X-ray analysis and X-ray diffraction pattern to study effect of olive oil on morphological and molecular behavior. The antimicrobial effects, viability and proliferation of as-spun bionanocomposite was studied using Escherichia coli (Gram negative) and NIH 3T3 fibroblasts as model strain and cell line respectively. The SEM-EDX and XRD results confirmed well oriented nanofibers and good dispersion of oil. The outcome of results also indicated that incorporation of olive oil in polymer media affected both the morphology and size of PU nanofiber membranes. The bionanocomposite was able to inhibit the growth of E. coli and revealed non-cytotoxic behavior towards the fibroblast cell culture. Thus the olive oil blended scaffold embedded with silver nanoparticles could be used as a prospective antimicrobial agent which can potentially reduce wound contamination and simultaneously help in wound healing process. Finally, our results clearly indicate the potential of designing bionanocomposite as medicated bandages for skin diseases, burns and damaged skin treatment. 2013 Elsevier B.V.Arora, S., Jain, J., Rajwade, J.M., Paknikar, K.M.Cellular responses induced by silver nanoparticles: In vitro studies2008Toxicology Letters179293100144http://www.scopus.com/inward/record.url?eid=2-s2.0-44449157591&partnerID=40&md5=962dd5430bff94dab7883b9cd8ab7f34ArticleScopusArora, S., Centre for Nanobioscience, Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, India; Jain, J., Centre for Nanobioscience, Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, India; Rajwade, J.M., Centre for Nanobioscience, Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, India; Paknikar, K.M., Centre for Nanobioscience, Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, IndiaA systematic study on the in vitro interactions of 7-20 nm spherical silver nanoparticles (SNP) with HT-1080 and A431 cells was undertaken as a part of an on-going program in our laboratory to develop a topical antimicrobial agent for the treatment of burn wound infections. Upon exposure to SNP (up to 6.25 g/mL), morphology of both the cell types remained unaltered. However, at higher concentrations (6.25-50 g/mL) cells became less polyhedral, more fusiform, shrunken and rounded. IC 50 values for HT-1080 and A431 as revealed by XTT assay were 10.6 and 11.6 g/mL, respectively. When the cells were challenged with 1/2 IC 50 concentration of SNP (6.25 g/mL), clear signs of oxidative stress, i.e. decreased GSH (2.5-folds in HT-1080, 2-folds in A431) and SOD (1.6-folds in HT-1080, 3-folds in A431) as well as increased lipid peroxidation (2.5-folds in HT-1080, 2-folds in A431) were seen. Changes in the levels of catalase and GPx in A431 cells were statistically insignificant in both cell types. DNA fragmentation in SNP-exposed cells suggested apoptosis. When the apoptotic thresholds of SNP were monitored with caspase-3 assay the concentrations required for the onset of apoptosis were found to be much lower (0.78 g/mL in HT-1080, 1.56 g/mL in A431) than the necrotic concentration (12.5 g/mL in both cell types). These results can be used to define a safe range of SNP for the intended application as a topical antimicrobial agent after appropriate in vivo studies. 2008 Elsevier Ireland Ltd. All rights reserved.Arvidsson, R., Molander, S., Sandn, B.A.Impacts of a silver-coated future: Particle flow analysis of silver nanoparticles2011Journal of Industrial Ecology1568448547http://www.scopus.com/inward/record.url?eid=2-s2.0-83055186584&partnerID=40&md5=75b4c91c5b3928dcbff4e357eede8485ArticleScopusArvidsson, R., Division of Environmental Systems Analysis, Chalmers University of Technology, Gothenburg, Sweden; Molander, S., Division of Environmental Systems Analysis, Chalmers University of Technology, Gothenburg, Sweden; Sandn, B.A., Division of Environmental Systems Analysis, Chalmers University of Technology, Gothenburg, SwedenSilver is a compound that is well known for its adverse environmental effects. More recently, silver in the form of silver nanoparticles (Ag NPs) has begun to be produced in increasingly larger amounts for antibacterial purposes in, for instance, textiles, wound dressings, and cosmetics. Several authors have highlighted the potential environmental impact of these NPs. To contribute to a risk assessment of Ag NPs, we apply a suggested method named "particle flow analysis" to estimating current emissions from society to the environment. In addition, we set up explorative scenarios to account for potential technology diffusion of selected Ag NP applications. The results are uncertain and need to be refined, but they indicate that emissions from all applications included may increase significantly in the future. Ag NPs in textiles and electronic circuitry may increase more than in wound dressings due to the limited consumption of wound dressings. Due to the dissipative nature of Ag NPs in textiles, the results indicate that they may cause the highest emissions in the future, thus partly confirming the woes of both scientists and environmental organizations. Gaps in current knowledge are identified. In particular, the fate of Ag NPs during different waste-handling processes is outlined as an area that requires more research. 2011 by Yale University.AshaRani, P.V., Hande, M.P., Valiyaveettil, S.Anti-proliferative activity of silver nanoparticles2009BMC Cell Biology1014716598http://www.scopus.com/inward/record.url?eid=2-s2.0-70449413628&partnerID=40&md5=6cb99eadbc0d58b205bb696a3c3ea4f2ArticleScopusAshaRani, P.V., Department of Chemistry, Faculty of Science, National University of Singapore, 3 Science Drive 3, 117543, Singapore, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 2 Medical Drive, 117597, Singapore; Hande, M.P., Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 2 Medical Drive, 117597, Singapore; Valiyaveettil, S., Department of Chemistry, Faculty of Science, National University of Singapore, 3 Science Drive 3, 117543, SingaporeBackground: Nanoparticles possess exceptional physical and chemical properties which led to rapid commercialisation. Silver nanoparticles (Ag-np) are among the most commercialised nanoparticles due to their antimicrobial potential. Ag-np based cosmetics, therapeutic agents and household products are in wide use, which raised a public concern regarding their safety associated with human and environmental use. No safety regulations are in practice for the use of these nanomaterials. The interactions of nanomaterials with cells, uptake mechanisms, distribution, excretion, toxicological endpoints and mechanism of action remain unanswered. Results: Normal human lung fibroblasts (IMR-90) and human glioblastoma cells (U251) were exposed to different doses of Ag-nps in vitro. Uptake of Ag-nps occurred mainly through endocytosis (clathrin mediated process and macropinocytosis), accompanied by a time dependent increase in exocytosis rate. The electron micrographs revealed a uniform intracellular distribution of Ag-np both in cytoplasm and nucleus. Ag-np treated cells exhibited chromosome instability and mitotic arrest in human cells. There was efficient recovery from arrest in normal human fibroblasts whereas the cancer cells ceased to proliferate. Toxicity of Ag-np is mediated through intracellular calcium (Ca2+) transients along with significant alterations in cell morphology and spreading and surface ruffling. Down regulation of major actin binding protein, filamin was observed after Ag-np exposure. Ag-np induced stress resulted in the up regulation of metallothionein and heme oxygenase -1 genes. Conclusion: Here, we demonstrate that uptake of Ag-np occurs mainly through clathrin mediated endocytosis and macropinocytosis. Our results suggest that cancer cells are susceptible to damage with lack of recovery from Ag-np-induced stress. Ag-np is found to be acting through intracellular calcium transients and chromosomal aberrations, either directly or through activation of catabolic enzymes. The signalling cascades are believed to play key roles in cytoskeleton deformations and ultimately to inhibit cell proliferation. 2009 AshaRani et al; licensee BioMed Central Ltd.Asharani, P.V., Lianwu, Y., Gong, Z., Valiyaveettil, S.Comparison of the toxicity of silver, gold and platinum nanoparticles in developing zebrafish embryos2011Nanotoxicology51435449http://www.scopus.com/inward/record.url?eid=2-s2.0-79952838115&partnerID=40&md5=55781141b46b48790d6643cac38736f9ArticleScopusAsharani, P.V., Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore; Lianwu, Y., Department of Biological Sciences, National University of Singapore, Singapore, Singapore; Gong, Z., Department of Biological Sciences, National University of Singapore, Singapore, Singapore; Valiyaveettil, S., Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, SingaporeNanoparticles have diverse applications in electronics, medical devices, therapeutic agents and cosmetics. While the commercialization of nanoparticles is rapidly expanding, their health and environmental impact is not well understood. Toxicity assays of silver, gold, and platinum nanoparticles, using zebrafish embryos to study their developmental effects were carried out. Gold (Au-NP, 15-35 nm), silver (Ag-NP, 5-35 nm) and platinum nanoparticles (Pt-NP, 3-10 nm) were synthesized using polyvinyl alcohol (PVA) as a capping agent. Toxicity was recorded in terms of mortality, hatching delay, phenotypic defects and metal accumulation. The addition of Ag-NP resulted in a concentration- dependant increase in mortality rate. Both Ag-NP and Pt-NP induced hatching delays, as well as a concentration dependant drop in heart rate, touch response and axis curvatures. Ag-NP also induced other significant phenotypic changes including pericardial effusion, abnormal cardiac morphology, circulatory defects and absence or malformation of the eyes. In contrast, Au-NP did not show any indication of toxicity. Uptake and accumulation of nanoparticles in embryos was confirmed by inductively coupled plasma optical emission spectroscopy (ICP-OES), which revealed detectable levels in embryos within 72 hpf. Ag-NP and Au-NP were taken up by the embryos in relatively equal amounts whereas lower Pt concentrations were observed in embryos exposed to Pt-NP. This was probably due to the small size of the Pt nanoparticles compared to Ag-NP and Au-NP, thus resulting in fewer metal atoms being retained in the embryos. Among the nanoparticles studied, Ag-NPs were found to be the most toxic and Au-NPs the non-toxic. The toxic effects exhibited by the zebrafish embryos as a consequence of nanoparticle exposure, accompanied by the accumulation of metals inside the body calls for urgent further investigations in this field. 2011 Informa UK, Ltd.valos Fnez, A., Isabel Haza, A., Mateo, D., Morales, P.In vitro evaluation of silver nanoparticles on human tumoral and normal cells2013Toxicology Mechanisms and Methods2331531602http://www.scopus.com/inward/record.url?eid=2-s2.0-84879574133&partnerID=40&md5=8d0864a8fc2b5006bc742ad70e3b0940ArticleScopusvalos Fnez, A., Departamento de Nutricin, Bromatologa y Tecnologa de Los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid 28080, Spain; Isabel Haza, A., Departamento de Nutricin, Bromatologa y Tecnologa de Los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid 28080, Spain; Mateo, D., Departamento de Nutricin, Bromatologa y Tecnologa de Los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid 28080, Spain; Morales, P., Departamento de Nutricin, Bromatologa y Tecnologa de Los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid 28080, SpainSilver nanoparticles (AgNPs), which have well-known antimicrobial properties, are extensively used in various medical and general applications. Despite the widespread use of AgNPs, relatively few studies have been undertaken to determine the toxicity effects of AgNPs exposure. The aim of the present work was to study how AgNPs interact with four different human cell lines (hepatoma, leukemia, dermal and pulmonary fibroblast) in order to understand the impact of such nanomaterials on cellular biological functions. For toxicity evaluations, mitochondrial function (MTT assay) and membrane leakage of lactate dehydrogenase (LDH assay) were assessed under control and exposed conditions (24, 48 and 72h of exposure). Furthermore, we evaluated the protective effect of N-acetyl-l-cysteine (NAC) against AgNP-induced cytotoxicity. Results showed that mitochondrial function decreased in all cell lines exposed to AgNPs at 6.72-13.45g/ml. LDH leakage also increased in all cell lines exposed to AgNPs (6.72-13.45g/ml). However, the cytotoxic effect of AgNPs (13.45g/ml) was prevented by pretreatment of different concentrations of NAC (1-20mM). Our findings indicate that AgNPs are cytotoxic on human tumor and normal cells, the tumor cells being more sensitive to the cytotoxic effect of AgNPs. In addition, NAC protects human cells from cytotoxicity of AgNPs, suggesting that oxidative stress is in part responsible of this effect. 2013 Informa UK Ltd All rights reserved.Bae, E., Lee, B.-C., Kim, Y., Choi, K., Yi, J.Effect of agglomeration of silver nanoparticle on nanotoxicity depression2013Korean Journal of Chemical Engineering302364368http://www.scopus.com/inward/record.url?eid=2-s2.0-84873525788&partnerID=40&md5=1f549c27174a619c2a1206ce6998297cArticleScopusBae, E., School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, South Korea; Lee, B.-C., National Institute of Environmental Research, Incheon, 404-708, South Korea; Kim, Y., Department of Chemical Engineering, Kwangwoon University, Seoul, 139-701, South Korea; Choi, K., National Institute of Environmental Research, Incheon, 404-708, South Korea; Yi, J., School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, South KoreaSilver nanoparticles (AgNPs) are used commercially in a variety of applications, including textiles, cosmetics, spray cleaning agents, and metal products. AgNP itself, however, is classified as an environmental hazard by Environmental Protection Agency (EPA, USA) Nanotechnology White Paper, due to its toxic, persistent and bioaccumulative characteristics when exposed to the environment. We investigated the cumulative mortality and abnormalities in Japanese medaka (Oryziaslatipes) embryos after exposure to AgNPs. Free AgNPs in solution have a high activity with respect to biological interactions regarding blocking blood flow and distribution of AgNPs into the cells from head to tail of hatched O. latipes. Interestingly, the agglomeration of AgNPs (loss of nanosized characteristics) played an important role in the environmental toxicity. The present study demonstrated that when the AgNPs were exposed in the ecosystem and then formed agglomerates, nanotoxicity was reduced. 2012 Korean Institute of Chemical Engineers, Seoul, Korea.Barani, H., Montazer, M., Samadi, N., Toliyat, T.In situ synthesis of nano silver/lecithin on wool: Enhancing nanoparticles diffusion2012Colloids and Surfaces B: Biointerfaces929159http://www.scopus.com/inward/record.url?eid=2-s2.0-84856117511&partnerID=40&md5=8b2645b5ddc8c6745b2b7e3cc19b03e7ArticleScopusBarani, H., Textile Engineering Department, Center of Excellence in Textile, Amirkabir University of Technology, Tehran, Iran; Montazer, M., Textile Engineering Department, Center of Excellence in Textile, Amirkabir University of Technology, Tehran, Iran; Samadi, N., Department of Drug and Food Control, Faculty of Pharmacy and Pharmaceuticals Quality Assurance Research Center, Tehran University of Medical Sciences, Tehran, Iran; Toliyat, T., Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranSilver nanoparticles are being used increasingly in various applications because of their antibacterial properties. It is necessary to lower their direct contact with the skin by embedding in a polymer reducing their side effects. In this study, silver nanoparticles were synthesized inside the wool fibers acted as a polyfunctional ligands. Lecithin as a biological lipid was used to enhance the diffusion of silver ions and nanoparticles into the wool fibers reducing cytotoxicity effects of the nano silver loaded wool. The highest loading efficiency and inhibition zone was observed on the wool with the highest lecithin concentration. Presence of lecithin reduced the rate of nano silver release which results in decreasing the specific coefficient of lethality. Also, the extracted solution of the synthesized silver nanoparticles on the wool has not altered the morphology of L929 fibroblast cells. 2011 Elsevier B.V.Bartomiejczyk, T., Lankoff, A., Kruszewski, M., Szumiel, I.Silver nanoparticles - Allies or adversaries?2013Annals of Agricultural and Environmental Medicine2014854http://www.scopus.com/inward/record.url?eid=2-s2.0-84875626822&partnerID=40&md5=52c81aad422c9e76cde2b17f6a54dd93ReviewScopusBartomiejczyk, T., Centre for Radiobiology and Biological Dosimetry, Institute for Nuclear Chemistry and Technology, Warsaw, Poland; Lankoff, A., Centre for Radiobiology and Biological Dosimetry, Institute for Nuclear Chemistry and Technology, Warsaw, Poland, Jan Kochanowski University, Kielce, Poland; Kruszewski, M., Centre for Radiobiology and Biological Dosimetry, Institute for Nuclear Chemistry and Technology, Warsaw, Poland, Independent Laboratory of Molecular Biology, Institute of Rural Health, Lublin, Poland; Szumiel, I., Centre for Radiobiology and Biological Dosimetry, Institute for Nuclear Chemistry and Technology, Warsaw, PolandNanoparticles (NP) are structures with at least one dimension of less than 100 nanometers (nm) and unique properties. Silver nanoparticles (AgNP), due to their bactericidal action, have found practical applications in medicine, cosmetics, textiles, electronics, and other fields. Nevertheless, their less advantageous properties which make AgNP potentially harmful to public health or the environment should also be taken into consideration. These nanoparticles are cyto-and genotoxic and accumulate in the environment, where their antibacterial properties may be disadvantageous for agriculture and waste management. The presented study reviews data concerning the biological effects of AgNP in mammalian cells in vitro: cellular uptake and excretion, localization in cellular compartments, cytotoxicity and genotoxicity. The mechanism of nanoparticle action consists on induction of the oxidative stress resulting in a further ROS generation, DNA damage and activation of signaling leading to various, cell type-specific pathways to inflammation, apoptotic or necrotic death. In order to assure a safe application of AgNP, further detailed studies are needed on the mechanisms of the action of AgNP on mammalian cells at the molecular level.Bocking, S.The smallest revolution2008Alternatives Journal3443032http://www.scopus.com/inward/record.url?eid=2-s2.0-50949100489&partnerID=40&md5=c88b3ed48369a4c732f554232ce49abbArticleScopusBocking, S., Trent University, Peterborough, ON, CanadaNanotechnology is one of the greatest revolutions in today's technology and it has now been predicted that it will also be a common thing for its uses. Nanotechnology is one-billionth of a meter in size and it comes in many forms as infinitesimal particles, films and coatings, structures or part of larger assemblies. Researchers are excited with its strange properties and the possibility of scaling it up. In addition, the industry has welcomed this nanotechnology as with the case of iPod Nano. However, some of its aspects that make it useful also make it risky. And most nanotechnologies rely on "free" nanoparticles that directly affects the skin as with the case of copper and silver nanoparticles that is toxic to aquatic life. A report made on 2007 shows that nanotechnology is quite risky with the lack of information regarding its toxicity. Thus, it is now more important to be cautious with the use of nanotechnology.Bhmert, L., Niemann, B., Thnemann, A.F., Lampen, A.Cytotoxicity of peptide-coated silver nanoparticles on the human intestinal cell line Caco-22012Archives of Toxicology867110711154http://www.scopus.com/inward/record.url?eid=2-s2.0-84866042682&partnerID=40&md5=3329ad125906c4a94ba86215c69b869dArticleScopusBhmert, L., Department Food Safety, Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany; Niemann, B., Department Food Safety, Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany; Thnemann, A.F., Federal Institute for Material Research and Testing, Richard-Willstaetter-Str. 11, 12489 Berlin, Germany; Lampen, A., Department Food Safety, Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, 10589 Berlin, GermanySilver nanoparticles are used in a wide range of consumer products such as clothing, cosmetics, household goods, articles of daily use and pesticides. Moreover, the use of a nanoscaled silver hydrosol has been requested in the European Union for even nutritional purposes. However, despite the wide applications of silver nanoparticles, there is a lack of information concerning their impact on human health. In order to investigate the eVects of silver nanoparticles on human intestinal cells, we used the Caco-2 cell line and peptide-coated silver nanoparticles with deWned colloidal, structural and interfacial properties. The particles display core diameter of 20 and 40 nm and were coated with the small peptide L-cysteine L-lysine L-lysine. Cell viability and proliferation were measured using Promegas CellTiter-Blue Cell Viability assay, DAPI staining and impedance measurements. Apoptosis was determined by Annexin-V/7AAD staining and FACS analysis, membrane damage with Promegas LDH assay and reactive oxygen species by dichloroXuorescein assay. Exposure of proliferating Caco-2 cells to silver nanoparticle induced decreasing adherence capacity and cytotoxicity, whereby the formation of reactive oxygen species could be the mode of action. The eVects were dependent on particle size (20, 40 nm), doses (5-100 g/mL) and time of incubation (4-48 h). Apoptosis or membrane damage was not detected. Springer-Verlag 2012.Cheng, X., Zhang, W., Ji, Y., Meng, J., Guo, H., Liu, J., Wu, X., Xu, H.Revealing silver cytotoxicity using Au nanorods/Ag shell nanostructures: Disrupting cell membrane and causing apoptosis through oxidative damage2013RSC Advances3722962305http://www.scopus.com/inward/record.url?eid=2-s2.0-84872728686&partnerID=40&md5=7d7918e5bbdf37f31730dcb384de636aArticleScopusCheng, X., Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100005, China; Zhang, W., Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100005, China; Ji, Y., CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, 100190, China; Meng, J., Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100005, China; Guo, H., Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100005, China; Liu, J., Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100005, China; Wu, X., CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, 100190, China; Xu, H., Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100005, ChinaSilver nanoparticles (NPs) have been widely explored as antibacterial and antifungal agents in households, cosmetics, food packaging, medical devices and even various wound care products. With the application of silver NPs in biomedicine expanding, the safety issue is attracting ever-growing attention. Although increasing literatures have evidenced that silver NPs induce reactive oxygen species (ROS) in mammalian cells and thus resulted in cell damage, mechanisms of how these nanoparticles initiate ROS accumulation are open questions. In this work, we found an important pathway of initiating ROS overproduction. Gold nanorod core/silver shell nanostructures (herein termed as Ag nanorods, AgNRs) were employed as a model for silver NPs and NIH3T3 fibroblast cell line was chosen as mammalian cell model. Upon exposure to the AgNRs, the cell membrane of the fibroblasts was disrupted along with generation of malondialdehyde (MDA), possibly due to the dissolution of silver ions from AgNRs surface. Consequently, actin depolymerization in the cytoskeleton connecting tightly with the membrane occurred. The membrane damage allowed calcium influx and induced intracellular calcium overload, which further caused ROS overproduction and mitochondrial membrane potential variation. All these complex stresses eventually resulted in cell death through apoptosis in a dose-dependent manner. 2013 The Royal Society of Chemistry.Chu, C.-Y., Peng, F.-C., Chiu, Y.-F., Lee, H.-C., Chen, C.-W., Wei, J.-C., Lin, J.-J.Nanohybrids of silver particles immobilized on silicate platelet for infected wound healing2012PLoS ONE76 e383602http://www.scopus.com/inward/record.url?eid=2-s2.0-84861899285&partnerID=40&md5=c675d31a0dfdfe2ea9932f936d496a3fArticleScopusChu, C.-Y., Department of Dermatology, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan; Peng, F.-C., Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, Taiwan; Chiu, Y.-F., Department of Dermatology, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan; Lee, H.-C., Department of Dermatology, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan; Chen, C.-W., Department of Dermatology, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan; Wei, J.-C., Institute of Polymer Sciences and Engineering, National Taiwan University, Taipei, Taiwan; Lin, J.-J., Institute of Polymer Sciences and Engineering, National Taiwan University, Taipei, TaiwanSilver nanoparticles supported on nanoscale silicate platelets (AgNP/NSP) possess interesting properties, including a large surface area and high biocide effectiveness. The nanohybrid of AgNP/NSP at a weight ratio 7/93 contains 5-nm Ag particles supported on the surface of platelets with dimensions of approximately 80801 nm3. The nanohybrid expresses a trend of lower cytotoxicity at the concentration of 8.75 ppm Ag and low genotoxicity. Compared with conventional silver ions and the organically dispersed AgNPs, the nanohybrid promotes wound healing. We investigated overall wound healing by using acute burn and excision wound healing models. Tests on both infected wound models of mice were compared among the AgNP/NSP, polymer-dispersed AgNPs, the commercially available Aquacel, and silver sulfadiazine. The AgNP/NSP nanohybrid was superior for wound appearance, but had similar wound healing rates, vascular endothelial growth factor (VEGF)-A levels and transforming growth factor (TGF)-1 expressions to Aquacel and silver sulfadiazine. 2012 Chu et al.Chun, K.-Y., Kim, S.H., Shin, M.K., Kim, Y.T., Spinks, G.M., Aliev, A.E., Baughman, R.H., Kim, S.J.Free-standing nanocomposites with high conductivity and extensibility2013Nanotechnology2416165401http://www.scopus.com/inward/record.url?eid=2-s2.0-84875660724&partnerID=40&md5=522a703cd5c6acc39703720cc82023b5ArticleScopusChun, K.-Y., Center for Bio-Artificial Muscle, Department of Biomedical Engineering, Hanyang University, Seoul 133-791, South Korea; Kim, S.H., Center for Bio-Artificial Muscle, Department of Biomedical Engineering, Hanyang University, Seoul 133-791, South Korea; Shin, M.K., Center for Bio-Artificial Muscle, Department of Biomedical Engineering, Hanyang University, Seoul 133-791, South Korea; Kim, Y.T., IT Fusion Technology Research Center, Department of IT Fusion Technology, Chosun University, Gwangju 501-759, South Korea; Spinks, G.M., ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, University of Wollongong, Wollongong, NSW 2522, Australia; Aliev, A.E., Alan G MacDiarmid NanoTech Institute, University of Texas at Dallas, Richardson, TX 75083, United States; Baughman, R.H., Alan G MacDiarmid NanoTech Institute, University of Texas at Dallas, Richardson, TX 75083, United States; Kim, S.J., Center for Bio-Artificial Muscle, Department of Biomedical Engineering, Hanyang University, Seoul 133-791, South KoreaThe prospect of electronic circuits that are stretchable and bendable promises tantalizing applications such as skin-like electronics, roll-up displays, conformable sensors and actuators, and lightweight solar cells. The preparation of highly conductive and highly extensible materials remains a challenge for mass production applications, such as free-standing films or printable composite inks. Here we present a nanocomposite material consisting of carbon nanotubes, ionic liquid, silver nanoparticles, and polystyrene- polyisoprene-polystyrene having a high electrical conductivity of 3700 S cm -1 that can be stretched to 288% without permanent damage. The material is prepared as a concentrated dispersion suitable for simple processing into free-standing films. For the unstrained state, the measured thermal conductivity for the electronically conducting elastomeric nanoparticle film is relatively high and shows a non-metallic temperature dependence consistent with phonon transport, while the temperature dependence of electrical resistivity is metallic. We connect an electric fan to a DC power supply using the films to demonstrate their utility as an elastomeric electronic interconnect. The huge strain sensitivity and the very low temperature coefficient of resistivity suggest their applicability as strain sensors, including those that operate directly to control motors and other devices. 2013 IOP Publishing Ltd.Chung, D., Khosla, A., Seyfollahi, S., Gray, B.L., Parameswaran, A., Ramaseshan, R., Kohli, K.Embedded process for flexible conductive electrodes for applications in tissue electrical impedance scanning (EIS)2011Proceedings of IEEE Sensors612737218931896http://www.scopus.com/inward/record.url?eid=2-s2.0-84856820981&partnerID=40&md5=91d077568b10cabebbd75799c85147acConference PaperScopusChung, D., Micro Instrumentation Lab., Simon Fraser University, Burnaby, BC, Canada; Khosla, A., Micro Instrumentation Lab., Simon Fraser University, Burnaby, BC, Canada; Seyfollahi, S., Micro Instrumentation Lab., Simon Fraser University, Burnaby, BC, Canada; Gray, B.L., Micro Instrumentation Lab., Simon Fraser University, Burnaby, BC, Canada; Parameswaran, A., Engineering Science, Simon Fraser University, Burnaby, BC, Canada; Ramaseshan, R., Medical Physics Group, BC Cancer Agency, Abbotsford, BC, Canada; Kohli, K., Medical Physics Group, Fraser Valley Cancer Center, Surrey, BC, CanadaWe present the fabrication and testing of micro-patternable flexible conductive nanoparticle composite polymer (C-NCP) electrode arrays for electrical impedance scanning (EIS). We attempt to minimize EIS issues of mechanical skin contact and resolution through the use of highly compliant micropatternable elastomeric C-NCPs. We anticipate an increase in spatial resolution as the electrodes can be patterned into high density arrays using a new multi-level process presented here for the first time. We characterize the conductivity of the electrodes (average resistivity of 2.9810 -4 ohm-m +/- 8.3% at 60 wt-% of silver nanoparticles), compare the baseline impedance map with a new circuit phantom, and demonstrate anomaly detection in a gelatin tissue phantom using highly flexible Ag/AgCl C-NCP electrodes. 2011 IEEE.Chung, D., Seyfollahi, S., Khosla, A., Gray, B., Parameswaran, A., Ramaseshan, R., Kohli, K.Initial experiments with flexible conductive electrodes for potential applications in cancer tissue screening2011Proceedings of SPIE - The International Society for Optical Engineering7929 79290Zhttp://www.scopus.com/inward/record.url?eid=2-s2.0-79957991643&partnerID=40&md5=3c5a00e79284e6e37aad6739172b2237Conference PaperScopusChung, D., Micro Instrumentation Laboratory, Simon Frasier University, Burnaby, BC, Canada; Seyfollahi, S., Micro Instrumentation Laboratory, Simon Frasier University, Burnaby, BC, Canada; Khosla, A., Micro Instrumentation Laboratory, Simon Frasier University, Burnaby, BC, Canada; Gray, B., Micro Instrumentation Laboratory, Simon Frasier University, Burnaby, BC, Canada; Parameswaran, A., Engineering Science, Simon Frasier University, 8888 University Drive, Burnaby, BC, Canada; Ramaseshan, R., Medical Physics Group, BC Cancer Agency-Abbotsford Centre, Abbotsford, BC, Canada; Kohli, K., Fraser Valley Cancer Centre, 13750 96th Avenue, Surrey, BC, CanadaWe present initial results on the fabrication and testing of micropatternable conductive nanocomposite polymer (C-NCP) electrodes for tissue impedance measurements. We present these proof-of-concept results as a first step toward the realization of our goal: an improved Electrical Impedance Scanning (EIS) system, whereby tissue can be scanned for cancerous tissue and other anomalies using large arrays of highly flexible microfabricated electrodes. Previous limitations of existing EIS system are addressed by applying polymer based microelectromechanical system (MEMS) technology. In particular, we attempt to minimize mechanical skin contact issues through the use of highly compliant elastomeric polymers, and increase the spatial resolution of measurements through the development of microelectrodes that can be micropatterned into large, highly dense arrays. We accomplish these improvements through the development of C-NCP electrodes that employ silver nanoparticle fillers in an elastomer polymer base that can be easily patterned using conventional soft lithography techniques. These new electrodes are tested on conventional tissue phantoms that mimic the electrical characteristics of human tissue. We characterize the conductivity of the electrodes (average resistivity of 7x10 -5 ohm-m +/- 14.3% at 60 wt-% of silver nanoparticles), and further employ the electrodes for impedance characterization via Cole-Cole plots to show that measurements employing C-NCP electrodes are comparable to those obtained with normal macroscopic metal electrodes. We also demonstrate anomaly detection using our highly flexible Ag/AgCl C-NCP electrodes on a tissue phantom. 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).Chunyan, W., Valiyaveettil, S.Correlation of biocapping agents with cytotoxic effects of silver nanoparticles on human tumor cells2013RSC Advances3341432914338http://www.scopus.com/inward/record.url?eid=2-s2.0-84881422074&partnerID=40&md5=e29a0c9ff105dd99b1ab5f5c973d728eArticleScopusChunyan, W., Department of Chemistry, Faculty of Science, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore; Valiyaveettil, S., Department of Chemistry, Faculty of Science, National University of Singapore, 3 Science Drive 3, Singapore 117543, SingaporeMetal nanoparticles are used in a wide range of commercial products such as cosmetics, food packaging and household detergents. Owing to interesting antimicrobial properties, silver nanoparticles (Ag NPs) are commonly used in many commercial products. Recently, green approaches using plant extracts at room temperature have been developed for the synthesis of Ag NPs. Here we explored a one-pot approach, which combines capping, reducing agents and templates in one bioextract for synthesizing water soluble Ag NPs. Ginger, coffee and mint extracts were used for the synthesis of water soluble Ag NPs. The as-synthesized nanostructures were characterized using transmission electron microscopy and UV-Vis spectroscopy. The toxicity of Ag NPs with different capping agents was studied using hepatocellular liver carcinoma cells (HepG2) and human cervical cancer cells (HeLa). The level of toxicity was evaluated using changes in cell morphology, cell viability and oxidative stress studies. Ag NPs caused a decrease in the amount of ATP in cells while plant extracts alone did not have significant effect on the amount of ATP. It is interesting to note that bioextract capped Ag NPs do not increase but decrease production of reactive oxygen species (ROS) in a dose dependent manner, which could mostly be attributed to the antioxidant activity of biocapping agents on the surface of nanoparticles. Ag-mint, Ag-ginger and Ag-coffee NPs treatment caused cell cycle arrest in the G2/M phase and Ag-mint NPs exposure resulted in cell cycle arrest in the sub G1 stage. Annexin-V propidium iodide staining showed a large amount of apoptosis in Ag-mint NPs treated cells. A possible mechanism of toxicity of Ag NPs resulted from interruption of ATP synthesis, which further caused DNA damage and cell death through apoptosis. A complete elimination of toxicity, especially at higher concentrations of Ag NPs has not yet been achieved. This journal is The Royal Society of Chemistry 2013.Das, S., Das, J., Samadder, A., Bhattacharyya, S.S., Das, D., Khuda-Bukhsh, A.R.Biosynthesized silver nanoparticles by ethanolic extracts of Phytolacca decandra, Gelsemium sempervirens, Hydrastis canadensis and Thuja occidentalis induce differential cytotoxicity through G2/M arrest in A375 cells2013Colloids and Surfaces B: Biointerfaces1013253367http://www.scopus.com/inward/record.url?eid=2-s2.0-84864443069&partnerID=40&md5=b3dd02b1c43817cd4cc01d0196062c95ArticleScopusDas, S., Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India; Das, J., Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India; Samadder, A., Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India; Bhattacharyya, S.S., Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India; Das, D., Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India; Khuda-Bukhsh, A.R., Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, IndiaThe capability of crude ethanolic extracts of certain medicinal plants like Phytolacca decandra, Gelsemium sempervirens, Hydrastis canadensis and Thuja occidentalis used as homeopathic mother tinctures in precipitating silver nanoparticles from aqueous solution of silver nitrate has been explored. Nanoparticles thus precipitated were characterized by spectroscopic, dynamic light scattering, X-ray diffraction, atomic force and transmission electron microscopic analyses. The drug-DNA interactions of silver nanoparticles were analyzed from data of circular dichroism spectroscopy and melting temperature profiles using calf thymus DNA (CT-DNA) as target. Biological activities of silver nanoparticles of different origin were then tested to evaluate their effective anti-proliferative and anti-bacterial properties, if any, by exposing them to A375 skin melanoma cells and to Escherichia coli C, respectively. Silver nanoparticles showed differences in their level of anti-cancer and anti-bacterial potentials. The nanoparticles of different origin interacted differently with CT-DNA, showing differences in their binding capacities. Particle size differences of the nanoparticles could be attributed for causing differences in their cellular entry and biological action. The ethanolic extracts of these plants had not been tested earlier for their possible efficacies in synthesizing nanoparticles from silver nitrate solution that had beneficial biological action, opening up a possibility of having therapeutic values in the management of diseases including cancer. 2012 Elsevier B.V.de Souza, A.L.R., Kiill, C.P., dos Santos, F.K., da Luz, G.M., Rocha e Silva, H., Chorilli, M., Gremio, M.P.D.Nanotechnology-based drug delivery systems for dermatomycosis treatment2012Current Nanoscience845125191http://www.scopus.com/inward/record.url?eid=2-s2.0-84863925462&partnerID=40&md5=76ed5a0855c1100bf2c9c6b0e54ed46fReviewScopusde Souza, A.L.R., School of Pharmaceutical Sciences, Paulista State University, Rodovia Araraquara-Jau km 01, CEP 14801-902 Araraquara, SP, Brazil; Kiill, C.P., School of Pharmaceutical Sciences, Paulista State University, Rodovia Araraquara-Jau km 01, CEP 14801-902 Araraquara, SP, Brazil; dos Santos, F.K., School of Pharmaceutical Sciences, Paulista State University, Rodovia Araraquara-Jau km 01, CEP 14801-902 Araraquara, SP, Brazil; da Luz, G.M., School of Pharmaceutical Sciences, Paulista State University, Rodovia Araraquara-Jau km 01, CEP 14801-902 Araraquara, SP, Brazil; Rocha e Silva, H., School of Pharmaceutical Sciences, Paulista State University, Rodovia Araraquara-Jau km 01, CEP 14801-902 Araraquara, SP, Brazil; Chorilli, M., School of Pharmaceutical Sciences, Paulista State University, Rodovia Araraquara-Jau km 01, CEP 14801-902 Araraquara, SP, Brazil; Gremio, M.P.D., School of Pharmaceutical Sciences, Paulista State University, Rodovia Araraquara-Jau km 01, CEP 14801-902 Araraquara, SP, BrazilDermatomycosis are fungal infections that involve the stratum corneum of the skin and the nails, hair, and surfaces of mucous membranes. Mycological infections represent important public health disorders, and their incidence has increased in recent years. This increase may result from a number of causes, such as an increase in the susceptible population, including the elderly and immunodeficient, and social and cultural exchanges associated with sports and the use of swimming pools. In immunodeficient individuals, the lesions associated with dermatomycosis are more intense, and what are initially superficial lesions can result in disseminated and fatal forms. The primary reasons for this include antifungal resistance, toxicity, lack of rapid and specific diagnoses and the poor penetration of drugs. The currently available antifungal agents for the treatment of dermatomycosis include azole and the allylamine group of drugs. The problems related to dermatomycosis therapy are the low residence times of the dosage forms in the site of action, side effects and variable drug permeability. Thus, novel topical drug delivery systems for antifungal therapy have been developed, including liposomes, niosomes, solid lipid nanoparticles, nanostructured lipid carriers, silver nanoparticles, microemulsion and liquid crystals. The objective of this study is to present a systematic review of nanotechnology-based drug delivery systems for dermatomycosis treatment. 2012 Bentham Science Publishers.Debabrata, D., Giasuddin, A.Cellular responses of Saccharomyces cerevisiae to Silver Nanoparticles2013Research Journal of Biotechnology8172771http://www.scopus.com/inward/record.url?eid=2-s2.0-84872069725&partnerID=40&md5=57e33b19f37711f7b08567cc710c4a89ArticleScopusDebabrata, D., Department of Biotechnology, Gauhati University, Guwahati-781014, India; Giasuddin, A., Department of Biotechnology, Gauhati University, Guwahati-781014, IndiaSilver nanoparticles are increasingly finding wide applications in health care, electronics and biomedical instruments as well as in consumer products like cosmetics. Although the bactericidal properties of silver nanoparticles are well documented, there is scant data available on their effect on eukaryotic cells. We report results from experiments concerning the model organism Saccharomyces cerevisiae and its growth in the presence of silver nanoparticles. A novel method of nanoparticle synthesis in yeast malt media was achieved. haracterization of particles by transmission electron microscopy and X-ray diffraction revealed a mean particle size of 8.6 nm. The minimum inhibitory concentration (MIC) and the minimum killing concentration (MKC) of the nanoparticles were 48.51?g/ml and 70.07?g/ml respectively. Growth of cells, in presence of nanoparticles, showed significant changes in growth rate and doubling time. Effect on total cellular protein, free amino acid and RNA content similarly showed deviations from cells devoid of nanoparticle stress. Electron micrographs revealed changes in plasma membrane which was further confirmed by an in vitro permeability assay. Saccharomyces cerevisiae could be an efficient model for study of cellular responses and toxicity of nanoparticles in eukaryotic cells.Devaraj, P., Kumari, P., Aarti, C., Renganathan, A.Synthesis and characterization of silver nanoparticles using cannonball leaves and their cytotoxic activity against MCF-7 cell line2013Journal of Nanotechnology2013598328http://www.scopus.com/inward/record.url?eid=2-s2.0-84883195237&partnerID=40&md5=c47448a9766ffcac597324179c21121fArticleScopusDevaraj, P., Department of Biotechnology, Faculty of Science Humanities, SRM University, Kattankulathur 603 203, Chennai, Tamil Nadu, India; Kumari, P., Department of Biotechnology, Faculty of Science Humanities, SRM University, Kattankulathur 603 203, Chennai, Tamil Nadu, India; Aarti, C., Department of Biotechnology, Faculty of Science Humanities, SRM University, Kattankulathur 603 203, Chennai, Tamil Nadu, India; Renganathan, A., Department of Biomedical Science, School of Basic Medical Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, IndiaCannonball (Couroupita guianensis) is a tree belonging to the family Lecythidaceae. Various parts of the tree have been reported to contain oils, keto steroids, glycosides, couroupitine, indirubin, isatin, and phenolic substances. We report here the synthesis of silver nanoparticles (AgNPs) using cannonball leaves. Green synthesized nanoparticles have been characterized by UV-Vis spectroscopy, SEM, TEM, and FTIR. Cannonball leaf broth as a reducing agent converts silver ions to AgNPs in a rapid and ecofriendly manner. The UV-Vis spectra gave surface plasmon resonance peak at 434 nm. TEM image shows well-dispersed silver nanoparticles with an average particle size of 28.4 nm. FTIR showed the structure and respective bands of the synthesized nanoparticles and the stretch of bonds. Green synthesized silver nanoparticles by cannonball leaf extract show cytotoxicity to human breast cancer cell line (MCF-7). Overall, this environmentally friendly method of biological silver nanoparticles production provides rates of synthesis faster than or comparable to those of chemical methods and can potentially be used in various human contacting areas such as cosmetics, foods, and medical applications. 2013 Preetha Devaraj et al.Dhar, S., Murawala, P., Shiras, A., Pokharkar, V., Prasad, B.L.V.Gellan gum capped silver nanoparticle dispersions and hydrogels: Cytotoxicity and in vitro diffusion studies2012Nanoscale425635674http://www.scopus.com/inward/record.url?eid=2-s2.0-84855581187&partnerID=40&md5=b8701e665a09de7573a23465a6442eb1ArticleScopusDhar, S., Materials Chemistry Division, National Chemical Laboratory, Pune 411 008, India, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune 411 038, India; Murawala, P., Materials Chemistry Division, National Chemical Laboratory, Pune 411 008, India; Shiras, A., National Centre for Cell Sciences, Pune 411 007, India; Pokharkar, V., Poona College of Pharmacy, Bharati Vidyapeeth University, Pune 411 038, India; Prasad, B.L.V., Materials Chemistry Division, National Chemical Laboratory, Pune 411 008, IndiaThe preparation of highly stable water dispersions of silver nanoparticles using the naturally available gellan gum as a reducing and capping agent is reported. Further, exploiting the gel formation characteristic of gellan gum silver nanoparticle incorporated gels have also been prepared. The optical properties, morphology, zeta potential and long-term stability of the synthesized silver nanoparticles were investigated. The superior stability of the gellan gum-silver nanoparticle dispersions against pH variation and electrolyte addition is revealed. Finally, we studied the cytotoxicity of AgNP dispersions in mouse embryonic fibroblast cells (NIH3T3) and also evaluated the in vitro diffusion of AgNP dispersions/gels across rat skin. 2012 The Royal Society of Chemistry.Dugosz, M., Bulwan, M., Kania, G., Nowakowska, M., Zapotoczny, S.Hybrid calcium carbonate/polymer microparticles containing silver nanoparticles as antibacterial agents2012Journal of Nanoparticle Research141213131http://www.scopus.com/inward/record.url?eid=2-s2.0-84869863844&partnerID=40&md5=e55fad9e259d7e55af642c58c85fc8d8ArticleScopusDugosz, M., Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland; Bulwan, M., Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland; Kania, G., Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland; Nowakowska, M., Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland; Zapotoczny, S., Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, PolandWe report here on synthesis and characterization of novel hybrid material consisting of silver nanoparticles (nAgs) embedded in calcium carbonate microparticles (-CaCO3) serving as carriers for sustained release. nAgs are commonly used as antimicrobial agents in many commercial products (textiles, cosmetics, and drugs). Although they are considered to be safe, their interactions with human organisms are still not fully understood; therefore it is important to apply them with caution and limit their presence in the environment. The synthesis of the new material was based on the co-precipitation of CaCO3 and nAg in the presence of poly (sodium 4-styrenesulfonate). Such designed system enables sustained release of nAg to the environment. This hybrid colloidal material (nAg/-CaCO3) was characterized by microscopic and spectroscopic methods. The release of nAg from -CaCO3 microparticles was followed in water at various pH values. Microbiological tests confirmed the effectiveness of these microparticles as an antibacterial agent. Importantly, the material can be stored as a dry powder and subsequently re-suspended in water without the risk of losing its antimicrobial activity. nAg/-CaCO3 was applied here to insure bacteriostatic properties of down feathers that may significantly prolong their lifetime in typical applications. Such microparticles may be also used as, e.g., components of coatings and paints protecting various surfaces against microorganism colonization. The Author(s) 2012.dos Santos, C.A., Jozala, A.F., Pessoa Jr, A., Seckler, M.M.Antimicrobial effectiveness of silver nanoparticles co-stabilized by the bioactive copolymer pluronic F682012Journal of Nanobiotechnology10433http://www.scopus.com/inward/record.url?eid=2-s2.0-84870062349&partnerID=40&md5=a32ce3f1108df5e3b8185801e4343baeArticleScopusdos Santos, C.A., Department of Chemical Engineering of the Polytechnic School, University of So Paulo (USP), So Paulo, Brazil; Jozala, A.F., Department of Biochemical and Pharmaceutical Technology, University of So Paulo (USP), So Paulo, Brazil; Pessoa Jr, A., Department of Biochemical and Pharmaceutical Technology, University of So Paulo (USP), So Paulo, Brazil; Seckler, M.M., Department of Chemical Engineering of the Polytechnic School, University of So Paulo (USP), So Paulo, BrazilBackground: Silver nanoparticles (AgNps) have attracted much interest in biomedical engineering, since they have excellent antimicrobial properties. Therefore, AgNps have often been considered for incorporation into medical products for skin pathologies to reduce the risk of contamination. This study aims at evaluating the antimicrobial effectiveness of AgNps stabilized by pluronic F68 associated with other polymers such as polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP).Methods: AgNps antimicrobial activity was evaluated using the minimum inhibitory concentration (MIC) method. The action spectrum was evaluated for different polymers associated with pluronic F68 against the gram negative bacteria P. aeuroginosa and E. coli and the gram positive bacteria S. Aureus.Results: AgNps stabilized with PVP or PVA and co-stabilized with pluronic F68 are effective against E. coli and P. aeruginosa microorganisms, with MIC values as low as 0.78% of the concentration of the original AgNps dispersion. The antimicrobial action against S. aureus is poor, with MIC values not lower than 25%.Conclusions: AgNps stabilized by different polymeric systems have shown improved antimicrobial activity against gram-negative microorganisms in comparison to unstabilized AgNps. Co-stabilization with the bioactive copolymer pluronic F68 has further enhanced the antimicrobial effectiveness against both microorganisms. A poor effectiveness has been found against the gram-positive S. aureus microorganism. Future assays are being delineated targeting possible therapeutic applications. 2012 dos Santos et al.; licensee BioMed Central Ltd.Falamas, A., Pinzaru, S.C., Dehelean, C.A., Venter, M.M.Raman imaging of in vivo damaged skin tissues from mice specimens2010Studia Universitatis Babes-Bolyai Chemia222732812http://www.scopus.com/inward/record.url?eid=2-s2.0-77957370127&partnerID=40&md5=38c42c47ca2e325abf6bb4afcb678aaaArticleScopusFalamas, A., Babes Bolyai University, Dept. of Physics, Kogalniceanu 1, RO 400084, Cluj-Napoca, Romania; Pinzaru, S.C., Babes Bolyai University, Dept. of Physics, Kogalniceanu 1, RO 400084, Cluj-Napoca, Romania; Dehelean, C.A., Victor Babe University of Medicine and Pharmacy, Faculty of Pharmacy, Eftimie Murgu Square 2, RO- 300041, Timioara, Romania; Venter, M.M., Babes Bolyai University, Faculty of Chemistry and Chemical Engineering, Arany Janos 11, 400028 Cluj-Napoca, RomaniaAutopsy skin tissues collected from mice specimens exposed to UVB irradiation and 7,12-dimethylbenz(a)anthracene were immersed in formalin solution mixed with colloidal silver nanoparticles and analyzed using Raman imaging. The aim of this study was probe the SERS technique applied to tissue analysis, to detect the main molecular components present in the investigated organs and to search f