institute of applied life sciences life science ... · institute of applied life sciences life...

Updated:

MasterManualofProceduresfor

HumanTestingCenter

InstituteofAppliedLifeSciences

LifeScienceLaboratoriesS360240ThatcherRoad,

Amherst,MA01003

atthe

UniversityofMassachusetts,Amherst

Updated:16October2017 ii

HumanTestingCenter

Chapter1:CoreAccess,Training,Scheduling,PricingandBilling

ExerciseInterventionandOutcomesCore

Chapter1:ParvoMedicsTrueOne2400MetabolicCart

Chapter2:VelotronBicycleErgometer

Chapter3:Monark828EBicycleErgometer

Chapter4:Monark874EBicycleErgometer

Chapter5:BiodexSystem4ProDynomometer

Chapter6:QuintonQ-Stressw/TM55Treadmill

Chapter7:GECarescapePatientMonitors

Chapter8:GELunariDXAa

LivingScienceCore

Chapter1:OxyconMobileMetabolicSystem

Chapter2:ActigraphLinkActivityMonitor

Chapter3:ActigraphGT3X+BTLEActivityMonitor

Chapter4:Actilife&ActilifeLiteSoftware

HumanMotionCore

Chapter1:QualysisOQUS700+/500+w/AMTIForcePlatformIntegration

Chapter1.1–CreatinganewprojectinQTM

Chapter1.2–QTMDataCollection

Chapter1.3-QTMDataProcessing

Chapter1.4–ExampleMarkersets

Chapter1.5–Visual3DTutorial

Chapter2:DelsysTrignoEMG/IMUunits–MotionCaptureIntegration

RoomCalorimeterCore

Chapter1:QuickGuide forValidation/Calibration

Updated:16October2017 HumanTestingCenter 1

MANUALOFPROCEDURES

HumanTestingCenter



Amherst,MA01003

atthe



UNIVERSITYOFMASSACHUSETTSAMHERST

INSTITUTEFORAPPLIEDLIFESCIENCES

HumanTestingCenter

CoreAccess,Training,Scheduling,Pricing,andBilling

MostrelevantinformationforgainingaccesstotheCoreswithintheHumanTestingCentercanbefoundontheCorewebsites.Accesswillonlybegiventousersonceallstepshavebeencompleted.

a) CoreAccess:

InternalUser.

IfyouareaUMassAmherst,UMasssystem,orFiveCollegebasedresearcherwhoisdoingresearchthatwouldreceive‘internal’pricing.ThefollowingstepsoutlinehowyouwillgainaccesstotheCoreFacilities.

i) ContactMichaelBusatheCoreFacilityDirectoranddiscussyourprojectsneedsandtimelines([email protected],413-755-0574).

ii) IfyouarenewtoIALSaMOUandpaymentauthorizationformmustbecompletedforeachcorefacilityyouwouldliketouse.ThePI’sStartUppaperworkcanbefoundintheFormstabofthewebpage.

ExternalUser

Ifyouareanexternaluserfromanacademicorindustrypartner,pleasecontactMichaelBusa,theCoreDirector,todiscussyourneeds([email protected],413-577-0574).

b) Training:IndividualswhowouldliketouseanypieceofequipmentintheHumanTestingCentermustcompleteatrainingsessionwithCorestaff(CoreRatesApply).ThesetrainingsserveasbothatechnicaltutorialforequipmentuseandanorientationforuserstotheHumanTestingCenter.Foruserswhoalreadyhaveexpertiseintheuseofaparticularpieceofequipment,theycanrequestthattheyjustdemonstrateproficiencyinuseandskipthetutorial.PleasenotethateventhoughausermaybeproficientinthetechnicalaspectsofacertainpieceofequipmentthattheintegrationofthebillinganddatastoragesystemsintheIALScoresdiffersfromstandalonepiecesofequipment.Beingtrainedontheseaspectswillensurethatdataisproperlystoredandbillingrecordsareaccurate.

Aspartofthetraining,theprospectiveusermustalsoprovidetheCoreStaffwithproofoftherequireEHStrainings.AllrequiretrainingsarelocatedatthebeginningofeachinstrumentsStandardOperatingProcedure.

c) Scheduling:AllschedulingofequipmenttimeistobedonethroughtheFacilitiesOnline

Managementsystem(FOM).i) Supervisors(i.e.PIs)musthavesupervisoraccountscreatedpriortohavingtheirRA’screate

profiles,theCoreDirectorwilldothisforthemfollowinganinitialemailorphonecallindicatingtheirintentiontouseacore.

ii) AllusersmustcreatetheirownFOMaccountandlinkittotheirsupervisor.


(1) Theymustthenclickontheindividualcapacitythattheywanttogainaccessto,andrequestatrainingtimewiththeCoreStaff.

(2) Duringregistration,theymustusethespeedtypethatmatchesthePaymentAuthorizationFormthatissubmittedaspartofthePI’sStartUppaperwork.Additionalspeedtypescanbeaddedbyusersinthecasewhentheyworkonmultipleprojects.

d) Pricing/Usage:PricingforfacilityusearepostedontheCorewebsites.i) Ratesaresetonanannualbasisandaresubjecttochange.ii) PricesaresetinFOM.ChargesforusearebasedonFOMactivetimeroundeduptothe

nextcompleteschedulingperiod.

e) Billing:BillingiscarriedoutbytheIALSaccountingstaff.i) Statementsaresentouttosupervisorsandtheirdesignatedrepresentative.ii) Disputesorchangesinspeedtypetotheinvoicemustbemadewithin10businessdays,

otherwisetheselectedspeedtypewillbeautomaticallychanged.

Updated:16October2017 ExerciseIntervention&OutcomesCore 1

ManualofProcedures

ExerciseIntervention&OutcomesCore



Amherst,MA01003

atthe




INSTITUTEFORAPPLIEDLIFESCIENCE

ExerciseInterventionandOutcomesCore

Chapter1:ParvoMedicsTrueOne2400

1.1Introduction:TheTrueOne®2400MetabolicMeasurementSystem(ParvoMedics,INC.,Sandy,UT,USA)sanintegratedmetabolicmeasurementsystemformaximalO2consumptiontestingandindirectcalorimetryassessment.TheTrueOne®2400'sAnalyzerModule,withparamagneticoxygenandinfra-redcarbondioxideanalyzers,isdesignedtobeaccurate,reliable,andeasytouse.Theuniqueflowmetercalibrationalgorithm,utilizinganimagereconstructiontechnique,correctsthenon-linearityofthepneumotachandprovideshighlyaccurateflowmeasurement.

Features:

•HighPrecisionFlowmeterCalibration

•CompactAnalyzerModule

•HighlyReliableMixingChamberPrincipleRecommendedApplications

•AthleticTesting

•ExercisePhysiologyTeachingandResearch

•SportsMedicineResearch

•EnergyExpenditureAssessment

•DifferentialDiagnosisinCardiopulmonaryDiseases


1.2HowtoCalibrate:Belowisastep-by-stepoutlineforcalibratingtheunit.

PreliminaryCalibrationSteps1. LocatethepowerswitchontherearoftheAnalyzerModuleandturniton.

a. Allowit30minutestowarmup

2. TurnonthecomputerandmonitorandlaunchTrueOnesoftware

a. Theydonotrequireawarmuptimeofmorethan2-minutes3. Assemblethewater-trapconnection

a. ConnectthefiltertotheT-connectorusingthegreyconnectionpiece.Gentlypushthetwotogetherinacircularmotion.


4. Attachthisassemblytothepneumotach.Attachbypushingthewatertraptothepneumotachwithslightpressure.Ensurethatthemixingchamber(tintedrectangularbox)doesnotfalloffthecart.

5. Ontheotherend(labeledtube)attachedoneendofthehose.


6. Next,assembletheT-mouthpiece3-Lsyringecalibrationassembly.Makesurethesmallwhitemembranesarefacingthesamedirection.Lookingatimage#3below,whenholdingtheunitwithupright,thesideofthebodywiththeair-flowarrowfacingup(smallarrowonthecenterbodypiece)themembraneonthissideshouldhavesurfaceparallelwiththelongwaterchamber.Themembraneontheoppositesideshouldbefacingthesameway.Ifyouarehavingtroubleusethisresource:https://www.youtube.com/watch?v=YSg65afxMCM

FINALPRODUCTforT-mouthpiece3-Lsyringecalibrationassembly


7. Taketheotherendofthehose(endnotattachedtothewatertrap)andconnectittotheT-mouthpiece3-Lsyringecalibrationassembly.Makesurethehoseisattachedtotheoutputvalve(endwith3metalrings).

8. Next,grabthe3-Lsyringe,ensuringithasthegreyconnectorattached


9. InterfacetheT-mouthpiece3-Lsyringecalibrationassemblywiththegreyendofthe3-Lsyringe.Makesurethattheconnectionistight,howeverdonotforcefullypushthemtogether.Rather,gentlymakesmallcircularmotionstoconnectthem.

10. Next,prepareforgascalibrationbyopeningthegascylinder.Turnthemetalknobonthetopofthe

cylindercounterclockwise1and½turns.TheCalGascalibrationiskeytoaccuratetestingresults.Youcanrunthegascalibrationasmanytimesasneededduringcalibration.Itisrecommendedtorunthecalgascalibrationonelasttimejustbefore(getsonthetreadmillorergometer)testingthesubject.WeneedtoensuretheCO2levelstheParvocartisreadingforroomairhavenotfloatedabove3-4%.Ifthishappens,theRERvalueinthetestwindowwithbeinflatedwhentheteststarts.


11. Nowyoucanattachthecalibrationgashose/whiteconnectorfromthecylindertotheCalGasPortonthemetaboliccart.Thisislocatedonthebackoftheunit.ItistotheleftoftheRoomAirportandjustabovetheO2Rangeswitch.Youmustholdthefrontoftheunit,soitdoesnotfalltothefloor,whilebringingthecalibrationgashose/whiteconnectortotheCalGasport.Stayparalleltothefloorandpushtheconnectorstraightintheportconnector.Toremove,pushdownlightlyonthemetalclaspandpullthecalibrationgashose/whiteconnectoraway.

12. Now,touchpneumotach,itshouldbewarm,indicatingitisshouldbewarmedup.Ensurethat30-

minuteshaspassed,andcontinewithcalibration

13. Thesystemisnowreadyforthethreestepcalibration.Oncethesystemhasbeensetup,thecalibrationprocessisveryquickandeasy.Thesystemshouldbecalibratedagaininbetweenmultiplesubjectsorifithasbeensittingturnedonbutnotusedforalongperiodoftime.Theonlycalibrationsteptheoreticallyneededafterinitialcalibrationistoapplythecalibrationgasagain.However,theenemyofthepneumotachismoisture.Excessmoisturewillelectricallydistortwhatthepneumotachissensing;thiswillbereflectedinerroneousreadingofthe3litersyringeas9,12,15litersetc...Thescreeninsidetheroundovenneedstobeasdryaspossible,soyoumayhavetousethesyringetoinputroomairacrossthesensorandintothemixingchamberifyouaretestingmultiplesubjects.ThepneumotachisverysensitivetosubtlechangesinCO2.Thisisthereasonwhyweneedtoletthepneumotachheatuptooperatingtemperature.DoingatestwithacoldpneumotachwillhavedistortedCO2results–theRERandVO2kgreadingswillbewayoff.


1.3FLOWMETERCALIBRATION

1. OpenTrueOnesoftwareandclick“FlowmeterCalibration”2. Enterroomtemperature,relativehumidity,andbarometricpressurereadingsfromtheVantage

VUEintodialogueboxonTrueOne

3. ClickandopenSampleBaselineintheupperrightcornerofthedialoguebox4. TheFlowmeterCalibrationProcessingwindowwillopen.Allinputstrokeswillbedonewiththe

3-Lsyringe.

5. Detectionstroke-oneinputstrokeindicatesairisflowingintothesystem(mouthpieceandallassembliesareproperlyconfigured).*NoticetheFlowindicatorwillshowtherealtimeinput


velocity,StrokePeakwithshowthepeakinrealtimeandintheblankbluefieldbesidetheCancelbutton,thevolumeofthesyringewillbedisplayedattheendofanystroke.Thisvalueshouldbearound3liters.Ifitreadshigh–9,12,15litersetc..thepneumotachhasamoistureissue(iftestingmultiplesubjectsoverashortperiodoftime)andmustbeallowedtodry.YoucanusethesyringesetuptodotheFlowmetercalibration,butdon'topenthesoftware.Youarejustphysicallydrivingairacrossthepneumotach,throughthemixingchamberandintothesystem

6. FlushStroke–fourinputstrokestopushfreshairintothesystem7. Thepneumotachcansense800lpm.Ofthefivecalibrationstrokesinputnext,twomustbeless

than80lpm.ThenumberwillfluctuatebesidetheFlownumericalindicator(totheleftofStrokePeak.ForExerciseStrokevelocityisveryveryslow,veryslow,slow,fast,faster.

8. Stroke1→approximately50-80lpm(very,veryslow)9. Stroke2→approximately100+lpm(veryslow)10. Stroke3→approximately200+lpm(slow)11. Stroke4→approximately300+lpm(fast)12. Stroke5→approximately400+lpm(faster)13. ForRestingStrokevelocitycanstayat50-80lpm14. Theresultissomethinglikethiscurve


1.4GASCALIBRATION

1. Onthehomescreen,click“GasCalibration”2. TheGasCalibrationwindowwillopen,likewithFlowmetercalibration,correctlyinput

temperature,humidity,andpressureintothedialoguebox.ClickOK.3. Theunitwillperformgascalibrationandafterabout20seconds.4. Apop-upwillappearpromptingyoutoturnoffthecalibrationgas.5. Asummarywindow(similartotheonepicturedbelow)willarise.Itisrecommendedtodothe

CalGascalibrationuntilyougettheexactnumbersonthecylinder.Also,youmaydothisstepjustbeforeyoursubjectisreadyfortesting

6. Click“Save”.7. Whendone,turnoffthecalibrationgascylinderbyturningclockwisehandtight.Thenbleedof

theexcessleftintheregulatorandthecalibrationgashose/whiteconnectorbygentlypushingontheplasticconnector/openingattheend.Bleedoffuntilbothindicatorsreadzero


1.5TESTINGwiththeParvo2400:1. Fitparticipantwiththeheartratemonitor,theheartratetransmitterislocatedonthereticulated

arm.Besuretowetthestraporapplyelectrodegelbeforethemonitorisused.2. Ifyouareusingareusingtheheadsupportsystem,attachedsweatguardtothefrontofthesystem.


3. Whiletheparticipantisseated,fittheheadsupportsothefrontportionisjustabovetheeyebrows.

Ensureasnugfit.Tightentheentirebandaroundtheheadusingtheknobontheback.Youwillbeusingamouthpieceandnoseclipwiththissetup.Attacharubbermouthadaptertothesmallwhitecylinderofthemouthpieceyoumadeearlier.Youwanttoavoidthesubjectbreathingoutofthecorneroftheirmouth(whenatmaximalexertion).Wewantatightsealregardlessofwhatdeviceisused-headsupportorfullmask.

4. Givethesubjectanosecliptoputon5. Theyarereadyforthetreadmillorergometer.Attachthehosetotheoutputendofthe

mouthpiece.Donotletthesubjectinserttheadaptoryet.Whenitistimetostartthetest,thesubjectcanjustspintheadaptortowardtheirface,placetheadaptorintheirmouthandbegin


mouthbreathingintothevalves.Wemustwaituntiltheappropriatetestwindowopensforthesubjecttodothis.Theyarenottobreatheintothehoseatthistime.

6. Thefullfacemaskdoesnotrequireanosecliptobeused.Tousethispiece,switchoutthesmall

whitecylinderforthesmallerclearadapter(picturedbelow).Threadtheendofthesmallclearadapterintothefrontholeofthebluefacemask.Facialhairmayinterferewithatightseal.Donothookupthehosetothefullfacemaskuntiltheteststarts.Thestrapswraparoundtheheadandthentheblackconnectorslockintotheclearbracketsofthemask.ThestrapsareadjustablebyslidingthestrapthroughtheblackconnectortheusingVelcrotokeepinplace.Themaskcoversthemouthandnose–sothestrapsneedtobetighttoensureagoodseal


1.6METABOLICTESTING1. Onthehomescreen,clickonVO2/MetabolicTesting.2. Inputparticipantinformation.Updatetemperature,humidityandbarometricpressureifithas

changed.Choosewhichdeviceyouareusing.

3. Ifyouusethetreadmillyouneedtochooseaprotocol.Youcancreateanewoneandnameitwhateveryouwant.Or,modifyanexistingoneandsaveit.Usewhatistherealreadyfromthepulldownmenu.OrmodifyaprotocolandnotsaveitbyjustclickingOK.

4. Treadmillprotocolscanhavearesting,warmup,exerciseandcooldownphase.Ifyoudonotwant

restingorwarmupphases,maketheduration=0.


5. ClickOK6. Ifyouusetheergometeryoualsoneedtochooseaprotocol.Youcancreateanewoneandnameit

whateveryouwant.Modifyanexistingoneandsaveit.Usewhatistherealreadyfromthepulldownmenu.OrmodifyaprotocolandnotsaveitbyjustclickingOK.

7. YouhavesimilaroptionswhenusingabikechooseaprotocolandpressOK.8. Nowthetestingwindowswillappear.Ifusingtheheadsupport,youcanattachthehosefromthe

reticulatedarm/T-connectortotheoutputconnectoronthemouthpiece.Thenoseclipcanbeworn.Donotletthesubjectbreathintothemouthpieceyet.Ifusingthefullfacemask,donotattachthehosefromthereticulatedarm/T-connectortothemaskyet.

9. Aseriesofsoftwarewindowswillopenandclose.10. Thenthiswindowwillopen.Youwillseetheblanktestresultswindowopenaswell.Thefieldswill

beblankbecausethesubjectisnotbreathingintothesystemyet.WaituntiltheCO2levelsexceeds0.03%,thenclickOK.

11. Ifusingtheheadgear,thesubjectcanspinthemouthpiecetowardtheirface.Theadaptorcanbe

placedinthemouthwhilethenoseclipison.Ifusingthefullfacemask,thehosefromthereticulatedarm/Tcannowbeattachedtotheinputconnectoronthefullfacemask.

12. Therewillbeadelayofapproximately15–20secondsbeforetheMetabolicMeasurementTesting

windowupdateswithdata.Thewhitespacebelowwillbefilledwithdataevery5seconds(thisresolutionlevelcanbechanged)


13. TheEventsbuttontotherightcanbeusedtoenterRPEvalues,increasedwattage,etc...apop-upwindowwillappear.SimplyentertheRPEvaluetotheemptydatafieldinthepop-up.Thisneweventwillbeaddedtothedatalinesinthewhitespacebelow

14. Welldocumentedreportsareavailableaftertesting.TotherightoftheCancelbuttonisasetoftimers.Thiswillshowoveralltesttime,restphasetimeleft,warm-upphasetime,andexercisephasetimeleftasyougothroughthestagesoftheprotocolyouareusing.Afterthetestyoucanviewdataunderthe“Reports”tabonthehomescreen.


1.7CLEANINGEQUIPTMENT1.) Whilewearinggloves,removeheadgearorthefullfacemaskfromthesubject.Rememberto

keepthesubjectattachedtothecartforabout20secondsafterthetesthasfinished.Thisallowssoftwaredelaytofillintheremainingdata

2.) Takeallcomponentsoffandbringtogreycart/sinkarea3.) Detachmouthpiecefromheadgear4.) Rinsethesweatguardunderwater.Theheartratestrapandtransmittercanberinsedaswell.

Letthesepartsairdryonthepeggedrack5.) DisassemblethemouthpieceandrinseallcomponentsinsinkwithJohnsonbabysoap

6.) Rinsein______________solution

1.8Contacts1. ParvoMedics

8152South1715EastSandy,UT84093,USA

1800-942-72552. MichaelBusa–CoreDirector

[email protected]


Chapter3:Monark828E,weightergometer3.1Introduction

MonarkErgometermodel828Eistheworld’smostcommonlyusedexerciseergometer.TheadjustablebrakesystemandtheforcecanbesetandreadKiloponds(kp)andNewton(N).Theresultoftheresistanceandcadenceisdisplayedinpower(Watts)ontheelectronicmeter.Thecycleisequippedwithanelectronicmetershowingpedalrevolutionsperminute(RPM),heartrateinbpm(HR),exercisetimeinminutesandseconds(TIME),speedinkmperhourormilesperhour(SPEED),covereddistanceinkmormile(DISTANCE),burnedCalories(CAL)andthepoweronthecycle(WATT).Thewattleveldependsonpedallingspeed,itcanbefinetunedbyincreasingordecreasingthespeedorpedalrpm.Each828Eiscalibratedatthefactory.ThismeansthatyoucanbegintousetheErgometerdirectlyafterassembly.However,iftheuserwishestoverifythescale,pleasereadtheinstructionfor“Calibration”inthismanual.

Quickfactsaboutthe874E:

•Large,well-balancedflywheel20kg(44lbs)

•Pendulumscale,easytocalibrate

•Adjustableseatheight

•Adjustablehandlebarwithquickreleaselever

•Stableframe,solidsteeltube

•Electronicdisplaywithheartrate

Corecompetencies:

1) Understandingthebrakedeviceandbasicsofthetensioncenter2) BefamiliarwiththeFitnesscomputerandwhatmetricsitgenerates3) Reviewserviceactionsandunderstandwhatactionsyoumightneedtotakebefore/afterusing

thisequipment.3.2UserInstructions

CycleadjustmentsSeatheightshouldbeadjustedtoacomfortableposition.Theappropriateheightistohavethekneeslightlybentwhenthesoleofthefootiscenteredoverthepedalaxlewiththepedalinthebottomposition.Toadjusttheseatheight,loosenthelever(1)ontheseattube.SeeFig:Adjustments.Alsonotethatthehandlebarstemshouldbeinsertedintotheframetubeatleast3inches.Thismeasureismarkedwith“MAX”onthestem(3).


FitnesscomputerTheMonark874EisequippedwithaFitnesscomputerthatdisplaysthefollowingmetrics:

1. Revolutionsperminute(RPM)2. Heartrateinbpm(HR)

a. Atthedisplayforheartrate(HR)aheartsymbolislitwhichmeansthatthemeteristryingtofindapulsesignalfromanexternalsource,chestbeltwithelectrodes,IfthemetercannotfindsuchasignaltheHRfunctionisautomaticallyturnedoffafter30seconds.Whenthefunctionisturnedofftheheartsymbolisnotlitanymore.Theheartratefunctioncanbeturnedonagainbypressingakey.

3. Exercisebouttimeinminutesandseconds(TIME)a. Thetimerstartsautomaticallywhenpedalsaremoved.MetervaluesforTime,Distanceand

CaloriescanbesettozerobypressingtheRESETbutton(seeimagebelowforRESTbuttonlocation)formorethantwoseconds.

4. Cyclingspeedinkmperhourormilesperhour(SPEED)5. Covereddistanceinkmormiles(DISTANCE)6. Caloriesburned(CAL)andpower(WATT)arealsodisplayedonthecomputer.Togetcorrect

readingsforcaloriesandwatts,thekp-valueontheelectronicmeter(Fitnesscomputer)hastobesettothesamevalueasthependulum,orthekpwindowshowntotheleftoftheelectronicmeter.

a. Forexample,thependulumandthekp-windowisshowing2kp.Pressthekp-keytotheleftonthemeter.Thelowerdisplaywindowisnowshowingfiguresinkp.Increaseordecreaseinstepsof0.1kpbypressingthekp-key-(arrowup)ortheRESET-key-(arrowdown)untilthereadingcorrespondswiththeactualordesiredkp-valuesonthependulumscaleorinthekp-window.AfterthatpresstheCAL/WATTbuttontoeithershowtheCAL-orWATT-figures.Thewattreadinginthedisplayisdependingonthepedallingspeed.Thewattreadingcanthenbefinetunedthroughincreaseordecreaseofthepedallingspeed.Caloriesarecalculatedallthetime.

Comment[MB1]:Makeavideo?

Comment[GP2R1]:Made.WaitingonYouTubeaccount.


Toactivateanyofthefunctionsdescribedabove,pressanykeyormovepedals.

Toreset,holdtheRESETkeyfortwoseconds.

BelowaresampleandimagesoftheFitnesscomputerandatableoutliningmetricsthecomputerdisplayswithcorrespondingunitsandranges.


Scale-zeroadjustmentLoosentensiondevicesothatthebrakebeltfeelsloose.Checkthatthependulumwillhanginverticalposition.

Scaleboard:Checkthattheindexonthependulum(2)weighisalignedwiththeindexatthe0-positiononthescaleboard.Ifadjustmentisnecessary,firstloosenthelocknut(1)andthenchangethepositionoftheboard.Tightenthelock-nutaftertheadjustment.Seefig:Scaleadjustments.

Kp-scale:Atthesametime,checkthatthekilopond-scale(5)totheleftoftheFitnesscomputer,shows0inlinewiththeindex.Toadjustthekp-scaleloosenthelockscrewforscaleindicator(9).Tightenthescrewfirmlyaftertheadjustment.Seefig:Calibration,Adjustmentkp-scalewindow.


CalibrationAlthoughallErgometersarecalibratedatthefactory,theusermaywishtoverifythisbyperformingamechanicalscalecalibration.Tocalibratefollowthestepsbelow:

1. Removethebluebrakebeltfromthespring2. Leanthebikeforwardsothatthecalibrationweight,4kg(4)ishangingindependentlyfromthe

framecover3. Besurethatthescaleboardissettozerobeforetheweightisputon,seesection”Scale-zero

adjustment”4. Putthe4kgweightonthespring(6).Whencorrectlyset,itshouldbepossibletoreadthisweight

fromthecorrespondingplaceonthescaleboard(7).Seefig:Calibration

Shouldtherebeadeviationfromthecalibrationweight,adjustthependulumtothecorrectweightonthescalebymeansoftheadjustingweightinsidethependulum(3).Seefig:Scaleadjustment.Tochangethepositionoftheadjustingweight,loosenthelockscrew(8)onthebackofthependulumweight.Shouldtheindexofthependulumweightbetoolow,movetheadjustingweightupwardsintheweight.Iftheindexshouldbetoohigh,theadjustingweightismovedsomewhatdownwardsandlockedinthenewposition.Repeatuntilthecorrectreading(4kg)isachieved.

Checkthecalibrationofthependulumweightonceayearorwhenneeded


SampleDataSheetforGradedExerciseTest

TIME DISTANCE SPEED HR RPM kP/meter/minàWatts(1kP/m/min=0.1634watts)

1:00

2:00

3:00

4:00

5:00

6:00

7:00


3.3Generaltroubleshootingguide

MaintenanceSchedule:

Serviceaction:

• Manufacturersuggestusingisopropylalcohol.Useadampbut,notwetclothtocleanthesurfaceyouwishtodisinfect.

• Periodicallywipethesurfacewitharustpreventative,especiallywhenithasbeencleanedandthesurfaceisdry.Thisisdonetoprotectthechromeandzincpartsaswellasthepaintedparts(4timesperyear).

• Checknowandthenthatbothpedalsarefirmlytightened.Ifnot,thethreadinginthepedalarmswillbedamaged.Also,checkthatpedalarmsarefirmlytightenedonthecrankaxle,tightenifnecessary.WhentheErgometerisnewitisimportanttotightenthepedalsafter5hoursofpedalling(4timesperyear).

• Checkthatthepedalcrankissecuretothecrankaxle(4timesperyear).• Besurethatthepedalsaremovingsmoothly,andthatpedalaxleisclearofdirtandfibres(4

timesperyear).• Whencleaningandlubricatingbesuretocheckthatallscrewsandnutsareproperlytightened

(2timesperyear).• Checkthatthechainissnugandthereisnoplayinthepedalcrank(2timesperyear).• Checkthatpedals,chainandfreewheelsprocketarelubricated(2timesperyear).• Besurethatthebrakebeltdoesnotshowsignificantsignsofwear(2timesperyear).• Checkthatthehandlebarsandseatadjustmentscrewsarelubricated(2timesperyear).• Besurethatallmovingpartsascrankandflywheelareworkingnormalandthatnoabnormal

playorsoundexists.(I.e.playinbearingscausesfastwearingandwiththatfollowsahighlyreducedlifetime.)

• Checkthattheflywheelisplacedinthecenterandwithplanerotation.• Thecrankandflywheelbearingsarelongtermgreasedanddonotrequiresupplementary

lubrication.Ifproblemariseswithcrankorflywheelbearings,contactMonarkdealer.• Replacebatteriesifthecomputerisnoton/seemstobeproducingincorrectmeasurements


3.4Contacts

1.)MonarkExerciseAB(Vabsbro,Sweeden)+46(0)281594940

2.) MichaelBusa–[email protected]


Chapter4:Monark874E,weightergometer

4.1Introduction:MonarkErgometermodel874Eisasafe,easy-tousebikeforfitnesstestingandworktests.Ithasabrakesystemwheretheworkloadisdeterminedbyweightsaddedintheweightbasket.Thepatentedweightbasketsystemdoesnotrequirecalibration,theprecisionoftheweightsensuresthattheworkloadiscorrect.

Quickfactsaboutthe874E:

•Large,well-balancedflywheel20kg(44lbs)

•Adjustablesaddleandhandlebar

•Stableframe,solidsteeltube

•Wheelsforeasytransport

•Electronicmeterwithheartrate

Corecompetencies:

1) Understandingthebrakedeviceandbasicsofthetensioncenter2) BefamiliarwiththeFitnesscomputerandwhatmetricsitgenerates3) Reviewserviceactionsandunderstandwhatactionsyoumightneedtotakebefore/afterusing

thisequipment


4.2Userinstructions

CycleadjustmentsSeatheightshouldbeadjustedtoacomfortableposition.Theappropriateheightistohavethekneeslightlybentwhenthesoleofthefootiscenteredoverthepedalaxlewiththepedalinthebottomposition.Toadjusttheseatheight,loosenthelever(1)ontheseattube.SeeFig:Adjustments.Alsonotethatthehandlebarstemshouldbeinsertedintotheframetubeatleast3inches.Thismeasureismarkedwith“MAX”onthestem(3).

FitnesscomputerTheMonark874EisequippedwithaFitnesscomputerthatdisplaysthefollowingmetrics:

1. Revolutionsperminute(RPM)2. Heartrateinbpm(HR)

a. Atthedisplayforheartrate(HR)aheartsymbolislitwhichmeansthatthemeteristryingtofindapulsesignalfromanexternalsource,chestbeltwithelectrodes,IfthemetercannotfindsuchasignaltheHRfunctionisautomaticallyturnedoffafter30seconds.Whenthefunctionisturnedofftheheartsymbolisnotlitanymore.Theheartratefunctioncanbeturnedonagainbypressingakey.

3. Exercisebouttimeinminutesandseconds(TIME)a. Thetimerstartsautomaticallywhenpedalsaremoved.MetervaluesforTime,Distanceand

CaloriescanbesettozerobypressingtheRESETbutton(seeimagebelowforRESTbuttonlocation)formorethantwoseconds.

4. Cyclingspeedinkmperhourormilesperhour(SPEED)5. Covereddistanceinkmormiles(DISTANCE)6. Workload(kp=weightbasket+weightinkg)canbesetwhichgivesareadingofcaloriesburned

(CAL)andpower(WATT).EnergyisexpressedinkJ(kilojoule)orcal(kilocalories,kcal).Conversion:1


kCal=4,2kJ.Powerisdependentoncadence,orpedalingspeed,whichmakesitpossibletoadjusttheworkload/powerbyincreasingordecreasingcadence.

a. Togetcorrectreadingsforcaloriesandwattsthekpvalueontheelectronicmeterhastobesettothesamevalueastheworkloadthatistheweightofthebasketincludingtheweightsinit.Therubberplatesareincludedinthecalibratedweightofthe1kgweightbasket.

i. Forexample,theworkloadis3kg(weightbasket0,1kg+2x1kgweight).Pressthekpkeytotheleftonthemeter.Thelowerdisplaywindowisnowflashingandshowingfiguresinkp.Increaseordecreaseinstepsof0.1kpbypressingthekpbutton(arrowup)ortheRESETbutton(arrowdown)untilthereadingiscorrespondingwiththeactualordesiredkpvalues(workload)fromtheweightbasket.Afterthat,presstheCAL/WATTbuttontoeithershowtheCALorWATTfigures.Next,presstheCAL/WATTbuttontoeithershowtheCALorWATTfigures.Refertosampleimagesbelow.

ii. Thewattreadingsinthedisplayaredependentoncadence.Thewattscanaccordinglybeadjustedbyincreasingordecreasingcadence.Caloriesarecalculatedinreal-time.

Toactivateanyofthefunctionsdescribedabove,pressanykeyormovepedals.

BelowaresampleandimagesoftheFitnesscomputerandatableoutliningmetricsthecomputerdisplayswithcorrespondingunitsandranges.


HeartrateUsingtheMonarkchest-strapheartratebeltresearcherscanmonitorparticipantheartrate.Thistechnologysenseselectricaloutputofthehearttocomputeheartrate.Properlyfittingthechest-strapisintegraltoprovidingaccuratereadings.

Beforeharnessingthechest-strap,cleanskinwherethebeltistobeplaced.Additionally,electrodesonthebacksideofthestrapshoulidebemoistenedwithwaterorspecializedgel.Thechest-strapshouldbesecuredatacomfortabletensionaroundtheparticipant’ssternum,seeFig:Placementofchestbeltforvisitation.Thebeltshouldbeplacedsuchthattheword“Monark”isoutwardfacing,upright,andcenteredontheparticipant’schest.InordertoproperlyconnectwiththeHRreceiveronthebike,thestrapshouldnotexceed100cmindistancefromthereceiver.Itisespeciallyimportantwhenfirstpairingthechestbeltwiththereceiver,thetwounitsarewithin60cmofeachother.

Figure:Placementofchestbelt

WingatetestingWhenusingthispieceforwingatetesting,resistanceintheweightbasketshouldbesetat0.075kgperkilogramoftheparticipant’sbodyweight.Whentestinghighly-trainedathletesconsiderusingahigherresistance,forexample1.0-1.3kgperkilogramofparticipantbodyweight.

Aftertheparticipanthascompleteda3-5minutewarmup,thetestshouldbegin.Afterthreesecondsofalloutpedaling,thebrakecordistobereleased,dosobylettinggoofcord.Thiswillapplyresistancetothesystem.The30-secondstestallowsforcalculatingthefollowingvariables:

1.) Peakpower(PP),inwatts2.) Relativepeakpower(RPP),inW/kg3.) Anaerobicfatigue(AF)4.) Anaerobiccapacity,inkilogram-Joules


Adjustingthebrakecord/belttensionImportantnote:Toreceivecorrectworkloadmetrics,itisimportanttoplacetheweightbasketaccordingtothedescriptionthatfollows.Ifthebaskethangstoolow,itmaytouchtheflywheel.Ifthebasketistoohigh,wrongworkloadinformationwillbedisplayed.

First,checkthatthebrakebeltislyingcorrectlyontheflywheelbrakesurface,seeimage1belowforproperalignment.Next,put4kgintheweightbasket(4),seeFig:Brakedevice,below.Whenrotatingtheflywheelbyhand,orwhenpulling/releasingthebrakecord,thebasketshouldlift/dropbetween40mmand60mm.Ifthisisnotthecase,thebrakebelthastobeloosenedortightenedalittleatthetensioncenter.

Loosenthecordbracket(#2inFigureBrakedevice)sothatthecordlengthcanbeadjusted.Ifthebasketistoolow,shortenbytensioningthecord.Alternatively,ifthebasketistoohigh,lengthenthecordbyeasingtension.

Toshortenorlengthentension,turnthetensioncenter(3)approximately45degrees,andmakeappropriatemodificationstocordlength.Afterthattightenthebracketagain,andcheckthatthedistancebetweentheweightbasketandflywheelisbetween40and60mmwhenyoupullonbrakecord.

Image1:Brakebeltpositioningontheflywheel


ReplacingthebrakebeltIfthebrakebeltneedstobereplaced,loosenthebrakecordandsetthebaskettoitsupperposition.Loosenthelockwasherthatisholdingthecord(#2)andremoveitfromthetensioncenter.Loosenorcutofftheknotontheotherendofthecordandthenremovethewholecordfromthebike.Whenassemblinganewbrakecord,firstenteroneendintotheholeinthetensioncenter,andtieaknotandlettheknotfallintothebiggerpartofthehole.Locktheendofthecordwiththelockwasher.


SampleDatasheetforWingateTest

Time RPM

0:05

0:10

0:15

0:20

0:25

0:30

4.3Generaltroubleshootingguide


4.4MaintenanceSchedule:

Serviceaction:

• Manufacturersuggestusingisopropylalcohol.Useadampbut,notwetclothtocleanthesurfaceyouwishtodisinfect.

• Periodicallywipethesurfacewitharustpreventative,especiallywhenithasbeencleanedandthesurfaceisdry.Thisisdonetoprotectthechromeandzincpartsaswellasthepaintedparts(4timesperyear).

• Checknowandthenthatbothpedalsarefirmlytightened.Ifnot,thethreadinginthepedalarmswillbedamaged.Also,checkthatpedalarmsarefirmlytightenedonthecrankaxle,tightenifnecessary.WhentheErgometerisnewitisimportanttotightenthepedalsafter5hoursofpedaling(4timesperyear).

• Checkthatthepedalcrankissecuretothecrankaxle(4timesperyear).• Besurethatthepedalsaremovingsmoothly,andthatpedalaxleisclearofdirtandfibers(4

timesperyear).• Whencleaningandlubricatingbesuretocheckthatallscrewsandnutsareproperlytightened

(2timesperyear).• Checkthatthechainissnugandthereisnoplayinthepedalcrank(2timesperyear).• Checkthatpedals,chainandfreewheelsprocketarelubricated(2timesperyear).• Besurethatthebrakebeltdoesnotshowsignificantsignsofwear(2timesperyear).• Checkthatthehandlebarsandseatadjustmentscrewsarelubricated(2timesperyear).• Besurethatallmovingpartsascrankandflywheelareworkingnormalandthatnoabnormal

playorsoundexists.(I.e.playinbearingscausesfastwearingandwiththatfollowsahighlyreducedlifetime.)

• Checkthattheflywheelisplacedinthecenterandwithplanerotation.• Thecrankandflywheelbearingsarelongtermgreasedanddonotrequiresupplementary

lubrication.Ifproblemariseswithcrankorflywheelbearings,contactMonarkdealer.• Replacebatteriesifthecomputerisnoton/seemstobeproducingincorrectmeasurements

4.5Contacts

1.)MonarkExerciseAB(Vabsbro,Sweeden)+46(0)2815949402.)MichaelBusa–[email protected]


Chapter5:VeltonCycleErgometerbyRacermate

5.1 Introduction-TheVelotronisalaboratory-gradecycleergometerthatdelivershighaccuracyandrepeatability.BuiltwithRacerMate’ssignaturedurability,enjoythemostrealistic,sciencegradetrainingexperienceontheworld’smostadvancedcycleergometer.Velotronisusedworldwidebythefinestuniversities,sportssciencelabs,andcoachingcenters,ofteninconjunctionwithpowerfulWingatesoftware.TheonlybicycleergometereverapprovedforuseintheUSACyclingNationalTalentSearch,Velotronisalsotheultimatecyclingtrainer.

TheVelotronisequippedwithaprecisionloadgenerator,providingsmoothvirtualshifting-pedalthroughthegearshift,withnowaitorfriction.Secondly,theradicalflywheeldesignallowsforendlesscoastingforatrueroadfeel,withacurrentdrivepowerfulenoughtodeliverthewidestspectrumofloadresistance.

POWERGENERATOR

FLYWHEEL


5.2CoreCompetenciesGettingstartedwithVelotronCoachingSoftware

1.) ConnectEXTPCcabletotheappropriatespotonthebackofthepowergenerator

2.) Attachoppositeend(stereotoUSBadaptor)toUSBonExercisetestinglaptop

3.) OpenVelotronCoachingSoftware(CS)2008fromthestartmenu

4.) GotoSourcemenuandselectReal-time5.) Ifthisisyourfirstuse,select“New”onsub-directoryofReal-timeinSourcemenu

a. Createauserdatafile,enteruserinformationandsaveàOK.OnsubsequentusesselectOpentochoosethisuserfile.Ifthereismorethanoneuser,createaUserDataFileforeachpersonwhowillusetheVelotron.


6.) GotoUtilitiesmenu,select“CommPorts”todeterminewhatserialportVelotronisconnected

to.Withthisinformation,selectSource-Real-time-CommPortandverifythecorrectportfoundinthetesthasbeenset.

7.) SelecttheOptionsmenutosetupyourManualErgosettings,ScreenCaptureOptions,Drag

Factor™settings,Reports,anddecidewhetheryouwanttorunthetestinMilesPerHourorKilometersPerHour.

8.) SelectStart,thenCharts,ManualErgo,Mode,orSpinScan.ByopeningChartsaselectionwindowforthecoursesortestsyouwanttouseforyourtestwillappear.Selectacoursefromthe“Courses”folderfromtheVelotronCS2008directory.ThenclickonOpen,theChartscreenwillappearwiththisselection.

9.) ByselectingacoursethiswillopenthedesiredcourseintheChartsScreen,clicktheredorgreenbuttonsontheleft-handsideofthechartwindowtoenableordisablethevariouschannelsofdata.

10.) HitF1ontheVelotronHandlebarControllertostartthetimer.Thecourseortestwillstart.11.) Belowisageneraloverviewofthechartfeatures:


a. FullarrayofdatatakendirectlyfromaVelotronsavedperformancefile.b. Current,PeakandAveragedisplayofdata.HRzone%andPulsePower™,Gearand

Gearing.c. Userselectablecolorgraphsthatcorrespondtothedatasection.d. TimeIndicatorBartopinpointexacttimecoordinates.e. Course/programprofileforRealTimeusetoindicatecurrentposition,orworkload.f. MultipleZoomLevelsinFileModeoperation.g. RPMzonepointertohelpmaintainafixedRPMduringanytest.Itisidealforfixed

wattagetests.Concludingatest

1.) Whenyouhavecompletedyourtest,youcaneitherresetthetimerandrunanewtest,orsavetheperformancedataasapermanentrecord.

a. Attheconclusionofaknownlengthtestadialogwillappearaskingyouto“ResetorSave.”

b. Ifthetestisopen-endedyouwouldpresstheF5(reset)buttontoprompta“Save,Reset,orCancel”(CanceladdedincaseyoupressedF5bymistake).ThesequestionscanbeansweredusingtheHandlebarControllerkeysshowninthedialogtoeliminatehavingtodismountthebike

Saving/Viewing/Exportingatest

PerformancefilesinCSaresavedas.velfiles.VelfileextensionsarecreatedwhetherthetestwasanERGfile,CRSfile,orsavedSpinScansession.TheperformancefileswillbesavedwithaUserName/Courseortesttype/Date/Time.velformat.1.) PressRESETafterthecompletionofatesttodisplaythe“Doyouwanttosave”dialog.

AnswerYes.2.) UsingtheHandlebarKeys,pressF1toSaveorF2nottosave.3.) Exportingthedata.SavedRaceFilesfromanyVelotronPCprogram(availableonyourhard

drive)canbeexportedasASCIItextfiles.Thiscanbedoneautomaticallyormanually.Ifyou


alwayswantanexportperformancefilesafteratest(forusein3rdpartyapplications)checktheAutoExportoptionintheExportOptionsmenu.FormanualexportchooseFile,thenselecteithertheExportfunctionorchangetheExportOptionsasneeded(check-markboth”shortformat”&“commadelimited”intheoptionsifyouplantousetheseexportfilesinaspreadsheet).Youwillbeaskedtoselecttheoriginalsavedperformancefile.Youcanthenbrowsetoanyfolderwherethefilemayreside.Onceexported,thesefileshavethesamenameastheoriginalfile,butwithanew.txtfileextension.

4.) Toopenaperformancereport,selectFile,OpenReport,andnavigatetothedesiredfileandselect.

FileMode,viewingsavedracefiles

1.) Select“SavedFile”fromtheSourcemenu.2.) SelectOptionsandcheckoruncheckMetricasdesired.3.) SelectStart,thenCharts,andyouwillbepresentedwithawindowwhereyoucanbrowseto

anyfolderfromthe“LookIn”dropdownwindowtoselectasavedperformancefile.Thiswillopeninthebackground,thereforeminimizeCS2008andopenthisdialoguebox.Oncethefileisfound,highlightitandselectOpen.Thechartscreenwillappearwiththeracedatashowinginit’sentirety.Toopenanotherfile,pressCtrl-O.

4.) Clicktheredorgreenbuttonsontheleft-handsideofthechartwindowtoenableordisablethevariouschannelsofdata.


5.) Youcannowmovethemouseleftandrightoverthedataandzoominandoutwiththeleftandrightmousebuttons.Right-clickyourmouseforFullZoomIn,andscrollforwardandbackthroughthedatabymovingtheTimeIndicatorBarfromtheleft-handorright-handsideoftheChartWindow,orestablishaZoomWindowusingtheleft/rightandup/downarrowkeysofyourPCkeyboardandhitEntertoactivatethislevelofzoom

Creatinganewcourse/programBecausecoursefilesmustretaintheformatasshownintheimageonthepreviouspage,itisrecommendedtoeditoneofthepreexistingcourseswhenmakinganewcourse.Thiswillalmostguaranteeausablecourseeverytime.

1.) TocreateanewcoursefromanexistingcoursewhileinNotepad,firstopenapreexistingcourse.

a. Toopenapreexistingcourse,openNotepadfromtheStartmenub. InNotepad,clickFile,thenOpenc. NavigatetoVelotronCS2008folderontheCdrive,thenopentheCoursessubdirectoryd. BydefaultNotepadlooksonlyfor“textfiles”,butifyouleft-clickonthedownarrowin

the“Filesoftype:”sectionandchoose“AllFiles(*.*)”,thenallthefilesinthisfolderwillappear.SelectthecoursetheyoudesiretoamendandclickOpen


e. WhenthecourseyouwanttoeditisopeninNotepad,renameitbyclickingFile,Save

As.TypeanewnameforthiscourseandchooseAllFiles(*.*)extensionwhensavingnewcourse.

f. LeftclickSavetomakea“carboncopy”of,andclose,thefirstopenedcourse.g. Nowyoucanproceedwithchangestomaketoyournewcourse:

i. Generally,toamendacoursechangeparametersfromtheoriginalcourse.Followtheformattinginstructionsoutlinedbelow:

h. Whenfinishedediting,clickFile/Save.


5.3GettingstartedwithVelotronWingateSoftware

1.) GototheStartmenuandopenVelotronWingate

2.) GotoClientmenuandselectNewDatabase


3.) Next,gotoClientmenuandselectNewClienta. Enterthedatabasethatyouwantdatatobesavedto,entersubjectantropometric

measuresandapplyoneofthefollowing:1.)torquefactor,2.)Torquevalue,3.)Droppedweight

b. OKtosave

4.) Now,gotoWingatemenu,andselectCreatenewprotocol

a. Createanameforprotocolandedittestingparametersb. Oktosavetodesiredpathway.Suggestedtosaveinclientdatafolder


5.) SelectWingatemenuandclickGraphScaling.EditWattsandHR/CadenceScaling6.) CheckthatVelotronispluggedintocomputer(seeabove).SelectSetupmenuandclickrunTest

CommPorts.NotewhichComminconnectedtoVelotron.ClickOK.Next,clickSetupmenu,andselectCommPort,verifythatthissettingmatchesTestCommPortsresults.Seeabovesectionforimages.

7.) GotoClientmenu,selectLoadClientandnavigatetoclientdata.a. GotoC-drive,VeloTronWingate2008folder,datafolder,selectyourdesireddatabase

folder,andselectandopenclientfolder


8.) Now,selectLoadProtocolfromWingatemenu.Navigatetotheprotocolyoumadeinstep4.

9.) Next,gotoWingatemenuandclickRunWingatea. TheVelotronisnowis“Warm-upmode.”Theclientcanwarm-upusingthepreselected

load-or-byusingtheUp/DownbuttonsontheWingateScreenorthoseontheVelotronHandlebarControlleradjusttheworkloadtosuitthewarm-uprequirements.Duetox-axisscalinglimitationsthereisonlyabout20secondsofwarm-upshown,butthewarm-upperiodpriortostartingtheclockisopen-ended.


10.) Whenyouarereadytobeginthetest,selecttheStartWingatebutton,thefirsticononthetoolbar

STARTa. Thewarm-upphasethatyouselectedwillbegin,thenaredcountdowntimerwill

appearintheupperrighthandcorner,indicatingwhentheloadwillbeappliedb. Attheendofthetestaplotoftheclient’sperformancewillappear.ClicktheReset

button,fourthbuttonfromtheleftonthetoolbar.Selectthepathwaywhereyouliketosavetheperformance.Suggestedsavinglocationistheoriginalclientdatafolder

RESETFilemode,viewingandexportingsaveddata

1.) ReturntotheWingatesoftwarehomepage2.) Toviewdatainfilemode,intheTree-viewdiagramontherightsideofthepageselectyour

database,thenclient,andfinallytheclientperformancefilea. Rightclickontheperformancefileandchooseplot.APlotwillappearintherightpanel.


3.) Toexportdata,returntothetree-viewdiagramandrightclick,butselectexport.a. Adialogueboxwillappearthatnoteswherethefilehasbeensaved

b. Theprogramwillexporttheperformancefileincommaseparatedtextformatintothesame

databasefoldertheoriginalperformancefilewasfound.Thefilewillhavethesamefilename,butwithaTXTfileextension.ThesefilescanbeusedMicrosoftexcel.

5.4Contacts

1.) Velotronforums:https://www.racermateinc.com/help-center-faq/2.) MichaelBusa–CoreDirector

413-577-0574 [email protected]


Chapter6:Quinton-55EKGStressSystem

6.1.Introduction-ImportanceofElectrocardiogramsAstheheartisamuscularpumpcomprisedoffourchambers,itrequireselectricalstimulationtocontrolthemuscularcontractionsandflowofbloodthroughoutthebody.Anelectrocardiogramisamethodtypicallyusedtoassesselectricalconductionintheheart.Thissimplenon-invasivetestallowstocheckfornormalrhythms,ratesandproperconductionofelectricalimpulsethroughoutthecardiactissue.ThismanualwillallowaresearchertounderstandandutilizetheEKGsysteminordertoobtainEKG’sformedicalinterpretation.Forallsubjects>70yearsofageorifasubjecthas≥2cardiovascularriskfactors,anEKGmustbecompletedpriortobeginningexerciseprotocols.

6.2EKGSettingsUnlessspecificallyrequestedotherwise,EKGsshouldberunwiththefollowingstandardsettings:PaperSpeed:25mm/sec

CalibrationStandard:1mV=10mmRhythmLead:DefaultLeadII

6.2.1SkinPrepPatientswithexcessivechesthairshouldbeshavedattheelectrodesite.Abradetheseareaswithfinesandpaperoranabrasivepad,andthencleanwithalcohol-saturatedgauze.Allowtheskintoairdrybeforeplacingelectrodes.Besurethesubjectdoesnothaveanyjewelrythatcouldinterferewiththetracings(necklaces,piercingsetc.).

6.3LimbLeadPlacementRA(White)–RightArmelectrodeisplacedonadistalportionoftherightlateralsideoftheupperarmbelowtheshoulder.LA(Black)–LeftArmelectrodeisplacedonadistalportionoftheleftlateralsideoftheupperarmbelowtheshoulder.RL(Green)–RightLegelectrodeisplacedontheinsidecalf,midwaybetweenkneeandankle.LL(Red)–LeftLegelectrodeisplacedontheinsidecalf,midwaybetweenkneeandankle.

6.4PrecordialLeadPlacementV1(COLOR)–4thintercostalplaceattherightmarginofthesternum.V2(COLOR)–4thintercostalplaceattheleftmarginofthesternum.V3(COLOR)–MidwaybetweenV2andV4(ontopofthe5thrib).V4(COLOR)–5thintercostalplaceattheleftmid-clavicularline.V5(COLOR)–AtthehorizontallevelofV4,attheleftanteriorline.V6(COLOR)–AtthehorizontallevelofV4,atthemid-axillaryline.


NOTE:LeadplacementdoesaffecttheEKGwaveform.Whenthelimbleadsareplacedonthetorso,waveformchangesmightbeseenintheQRSamplitude,axisshiftoccurs,Qwavescanbeseen,andTwavesmightappearflippedorflattened.Thesechangesareclinicallysignificantinthattheyareassociatedwithcardiacischemia.Ifanon-standardleadplacementisused,notethevariationintheEKGcommentfield.

6.5 InputtingaNewPatient

a. ToinitializetheQ-stressprogram,clickontheicononthedesktop


b. Tofirstsearchforpreviouspatients,clickthepatienticon

c. SearchforthepatientnameorMRN(MedicalRecordNumber)


d. Ifpatientinformationisnotfound,Select“New”andinputpatientdata

e. Thefollowingscreenwillallowyoutoenterallpossibledemographicinformation.ClickOKwhencompletedtocompletethedataentry.Alsonotethereshouldbenochangesmadetoprotocol,procedure,orreportunlessinstructed.


6.6 CheckLeadPlacementandArtifact

a. Afterpatientdataiscomplete,clickonthelightblueicontocheckleadplacementandconnections.

b. Click“Start”andwaitfortheiconstochangecolor

NOTE:Leadsshouldbeinthe“good”range

c. IfLeadsarenotwithinacceptable“good”range,removeleadsandpreptheelectrodesitesagain


d. Clickexitafterallleadsareproperlyplaced.

6.7 BaselineWaveforma. Aftertheleadsareplacedandthepatientdataisinput,clickontheresticontobeginrestingwaveforms.


b. ThescreenwillbegintodisplayheartrateandEKGwaveforms.Allowsabout10secondstopassbeforecollectingarestingEKG.

c. PressF5toprintoutafull12-leadrestingEKGd. PressF6toprintthecurrent3-leadscreene. Tobeginexerciseprotocolsclickonthe“startbelt”iconfollowedbythestartexercisebutton


f. Toendexerciseprotocolsclickthestartrecoverybuttonfollowedbythe“stopbelt”button

g. ToreviewthecollectEKGsclickthereviewtabandclick“Yes”


h. Clickyeswhenenteringreviewmode.Thiswillendthetest.

i. Whenthetesthasended,ifareportisneedclick“PartialReport”


Thiswillprintasummaryreportoftheprocedure

j. Whenfinishedreviewing,click“Exit”tosavethetest.Whenprompted,besurePDFisclickedyes,andpress“OK”

k. Toexitcurrentpatient,press“Exit”again


Afterobtainingabaselinewaveform,theEKGshouldbeinterpretedbyamedicalprofessional.Theinterpretationwillbecompletedbyeitheracertifiedclinicalexercisephysiologist(CCEP)ormedicaldoctor(MD)withinthedepartment.Anexercisephysiologistmayinterpretthebaselinewaveformswithsomerestriction.IftheEKGisdeterminedtobesatisfactoryandwithoutcontraindicationstoexercise,theexerciseportionwillresumewithoutreferralfromthemedicaldoctor.However,iftheexercisephysiologistdeterminesthatthebaselinewaveformisquestionableinanyway(basedoncurrentACSMrecommendationstoexercise),themedicaldoctorshouldbeconsultedtoverifytheEKG.Thedoctorwillthendeterminewhetherornottoproceedwithtesting.QuestionablebaselineEKG’scanincludebutarenotlimitedto:frequentectopy,abnormalheartrates(<40bpmor>100bpmatrest),STsegmentabnormalities<2mmdepressionorconductiondelays.Subjectswithsignificantabnormalitieswillbeexcludedfromthestudy.SignificantEKGabnormalitiesmayincludebutarenotlimitedto:dysrhythmias,electronicallypacedrhythms,conductionblocks,>1mmSTsegmentelevationsor>2mmSTsegmentdepressions.

6.8 ExerciseProtocols

AsthereisnocontinuousEKGmonitoringduringCADENCE-Adults,protocolisnotsignificantwhencollectingEKG’s.EKGwillbean“asneeded”proceduretoensuresafety.

6.9 Follow-Up

Ifatanytimeduringtestingprotocols,asubjectbecomesdizzy,lightheadedorcomplainsofchestpain,afollow-upEKGshouldbeperformedalongwithbloodpressures.InterpretationoftheEKGshouldbeimmediatelyreferredtotheExercisePhysiologistorMDonsite.


6.10 Cleaning

Allwiresandconnectionsshouldbewipeswithdisinfectingwipes.Usedelectrodesshouldbethrownaway.Thetreadmillandanyotherequipmentassociatedwiththetestshouldbethoroughlycleanedbetweenparticipantswithalcoholwipes.


ChapterX:

BiodexSystem4ProDynamometer

Turningthingson:

1.MakesuretheBiodexispluggedin.

2.Turnonthemainpowerswitch(locatedonthebottomleftofthebackoftheBiodexcart)byflippingitfromOtoI.

3.Turnonthepowerforthecomputeranddynamometer(greenswitchesonthebottomrightofthebackoftheBiodexcart)byflippingthemfromOtoI.

4.Turnonthecomputertower.

BasicsofsettinguptheBiodex

ThedynamometerandtheBiodexchaircanbetranslatedinspacesidetosideandverticallyandrotated.Thefollowingimagespointouthowtomovethechairanddynamometer.


Tiltthedynamometerheadbylooseningthisknob.

Comfortstopbutton.Pushingthisbuttonwillimmediatelystopatestandexitoutoftestingmode.Useasakillswitchonly.


Thisknobisusedtotilttheseatback

Thisleverisusedtorotatetheseat

Whenputtingattachmentsondynamometer,thesedotsmustalign.Youcanrotatethedynamometerdotusingthebluebuttonsonthedynamometerhead

Watchout,fingers/handsarecrushable!

Attachmentswithoutanutonthemwillusetheattachmenthandlestoredonthedynamometerhead(seeabove)Lettercorrespondstoside

(left/right)you’retesting


RunningaTestinBiodexAdvantage:

TheseinstructionswilltakeyouthroughrunningatestontheBiodex.IfyouwishtousetheExerciseorBiofeedbackmodes,pleaseseetheinstructionsintheBiodexusermanualorhelpdocumentation.

1.Whenthecomputerbootsup,itwillaskyoutoinitializethedynamometer.Removeanyattachments(theattachmentpostwillrotate)andclickOK.Thedynamometerwilltheninitialize.

2.BiodexAdvantageusuallybootsuptothelasttestthatwasrun.

A.Ifyouneedtoseehowtosetupthebiodexattachmentsforagiventest,clickthe“Anatomical”button.Inthewindowthatpopsup,youcanselectthejointandjointmotionyou’retestingtoseeavideoofhowtosetupthemachine.


B.Beforestartinganewtestyouneedtoloada“patient”andselectaprotocol.Follow thebuttonsontheleftfrom1-4.Asyougothroughthesteps,theactivewindowwillbe yellow.

1.Selectajointandmotion2.Selectamethodtoviewtheinstructions

3.Clickview


3.ThePatientwindowgivesyouoptionstoopenanexistingpatient,addapatient,oreditanopenpatient.

Tostartatest,clickherefirst

Toviewinstructionsforhowtosetupagiventest,clickhere

Notreadybecauseyouhavetogothroughthetestsetupfirst


A.Ifyouclick“Open,”youcanseealistofexistingentriesandselectonetocreateanewtestingsessionfor.Ifyouselectapatientfromthislist,youcanbeginanewtestbyclickingthe“NEW”buttonorEDITthepatient’sinformation.Ifapatienthasexistingtests,youcanREPEAToneofthese,printareportforanexistingtest,orDELETEanexistingtest.

B.Ifyouclick“AddPatient,”thewindowwillchangetotheviewbelow.Atminimum, youmustfilloutanyinformationboxesthatareinred.NOTE:Personalidentifying informationshouldneverbeenteredintotheBiodexsoftware.Onlyenterstudy participantIDsintonamefields.Neverenternames,birthdates,age,address,phone number,orotherinformationthatcouldidentifyanindividual.Weightandgenderare requiredbyBiodexandcanbeentered.


C.Afterenteringallnecessarypatientinformation,click“Save,”andthenclickon “Protocol.”


4.Hereyoucanloadaprotocol,addaprotocol,oreditaselectedprotocol.

Optionbuttons


A.Ifyouclickthe button,thefollowingwindowwillpopup.Hereyoucanviewbuilt-inorpreviouslydesignedtestingprotocols.


B.Ifyouwanttocreateanewprotocol,clickthe button.Thiswillactivatethe editableportionsoftheprotocolpane.

i.Dependingonthe“StudyType”and“Mode”optionsyouchoose,theotheroptionsintheblueboxabovewillchange.Forexample,ifyouchangefromIsometricmodetoIsokineticmode,insteadof“#Positions,”thegreenboxwillbelabeled“#Sets.”

Ifyouclickthis,theseoptionsbecomeavailable


5.Afteryouselectaprotocol,clickonthe“SetROM”button.Atthispoint,thechairanddynamometershouldbeinposition,thecorrectattachmentshouldbeonthedynamometer,andtheparticipantshouldbeseatedandstrappedin.

A.Belowisthe“SetROM”window.Followtheblinkingredtextthrougheachstep.

1.First,selectthesidebeingtested

Usingthisoptionisnotrecommended(generallyonlyavailablewhenswitchingfromonesidetotheotherinbilateralprotocols)

2.ClickCLEARLIMITS.SetAWAYandTOWARDlimitbymovingparticipant’slimbtothesepositions,holdingthere(seebelow),andclickingtheSETbutton.Notethatthissetstherangethattheparticipantwillbetestedin.

3.MovelimbtoAnatomicalReferenceposition,holdthere,andclicktheCalibratePositionbutton.AnatomicalReferencecanbechangedbyclickingonthebuttonthatreads“90”here.

4.MovelimbtoAWAYlimit,holdthere(usingholdbutton),haveparticipantcompletelyrelaxlimb,andclicktheLimbWeightbutton.Ifyougetanerroronthis,seetextbelowini.

5.ClickContinueViewSetupbuttonwillshowaschematicofwhat“AWAY”and“TOWARD”are,aswellashowanglesarereferenced


i.Clicking“LimbWeight”maygivethefollowingerror:

ii.IfthisoccursandthelimbwasweighedattheAWAYlimit,youwillneedtoincreasetheAWAYlimit,movethelimbtothisposition,andweighagain.IftheAWAYlimitisalreadyattheparticipant’slimitforthegiventest.

6.Afterhitting“Continue”intheSetROMwindow,theprogramwillmoveontotesting.TheprogramwillswitchtothefollowingwindowandwaitthereuntilyouhitSTART.IfStartisnotanoption,itmeansyoumissedsomethingbetweenstep3andthisstep.Whenyouarereadytobeginyourtest,clickSTART.

A.Ifyouwanttochangethevisualizationforatest(e.g.,bargraphinsteadofline),clickonthe

options button.

Holdbuttoncanholdparticipant’slimbinaposition.


B.First,atrialrepswindowwillappear.Here,theparticipantcan practicethemotionandspeedofthetestbeforetheprogramstarts collectingdata.Whenyou’rereadytostarttherealreps/sets,have theparticipantholdtheirlimbinthestartposition(usually TOWARDlimit).

C.Oncetheparticipantholdsatthestartpositionforasufficient time,thetestwillbegin.Youwill seethiswindow:

Startbutton


D.Oncethetestbegins,youwillseereal-timedataofthetorquetheparticipantis generating,aswellasprogressthroughthecurrentsetviathePieChart.

E.Duringrestperiodsbetweensetsorsectionsofaprotocol,the windowtotherightwillcountdowntothenextset.

7.Whenatestisdone,youcanclickontheReportbuttonortheCurvebuttontoviewresults.

A.Thereportwindowgivesoptionsforgeneratingstandardreports.Thisisaneasywaytogiveparticipantsfeedbackontheirperformance.Hereyoucanpreviewareportorprintareport.Ifyouwillnotbeprintingareportandalltestsarecomplete,thetestingsessionisover.


B.ToanalyzeresultsinBiodexAdvantageorexportdatatoanASCIIformat,clickontheCurvebutton.

i.Therearemanyoptionsforreal-timeanalysisinthecurvewindow.Thesearepointedoutbelow.

ii.DataexportedfromtheCurvewindowcanbetakenoffthecomputeronaUSBdriveandanalyzedusingothersoftware(e.g.,MatLab,Excel).

Clickheretoexportdata

Enablecursors(bluelinesingraphabove)andusebuttonsheretoselectrangesofdata

Optionsforadjustinggraphdisplayandperformingsomereal-timeanalysis.Wedonotrecommendusingthe“Filtered”optionexceptfordemonstrationpurposes

Changebetweentestshere

Updated:16October2017 RoomCalorimeterCore 76

MANUALOFPROCEDURES

HumanMotionCore



Amherst,MA01003

atthe



Chapter1QualisysOqus700+/500+w/AMTIForcePlatformintegration

Chapter1.1-CreatingaNewProjectinQTM

WhenbeginninganewstudyyouwillneedtocreateanewprojectinQTM.Thisprocessensuresthatyoudon’tinadvertentlyalteranotherstudy’sprojectsettingsandalsocreatesadesignateddirectoryforyourstudy’sdata.

1.First,openQTM

2.AwindowdisplayingcurrentprojectswillpopupwhenyouopenQTM.Whencreatinganewproject,click“Newproject…”

3.Thefollowingwindowwillpopup.In“Projectname:”typethenameofyourstudyorthenameyouwouldliketobeappliedtoyourdirectory.Notethatwhenyouchangethisname,theendofthe“Projectfolderpath:”willchange.Ifyouwouldliketheprojectfoldertobelocatedsomewhereotherthanwhereisindicatedbythe“Projectfolderpath:”youcanbrowsetothatlocationusingthe“Browse…”button.Whenyournameandpathareset,select“Settingsimportedfromanotherproject”andhit“OK.”


4.Anotherdialogwindowwillopenaskingyoutoselecta“Settings”filetobaseyournewprojecton.NavigatetoC:\Users\Qsys02\Documents\Project_Starterandselectthe“Settings”filefoundthere.Theseprojectsettingshavebeencreatedspecificallytoactasastartingpointfornewprojects.

Typeyourdesiredprojectnamehere

Checkthispath

Usethisoption


5.Afterclicking“Open,”QTMshouldstartup.EnsurethatyouareinyournewprojectbycheckingthetopbarontheQTMwindow.Itshouldnowread[Project:Yourprojectname]

Additionally,thereshouldnowbeafolderatthepathyoupreviouslydesignatedthathasthefollowingfolders/filesinit:

6.Afterverifyingthatyournewprojecthasbeencreatedcorrectly,youcanbeginchangingsettings.Todo

this,firstclickontheprojectoptions button.Thereareseveralprojectsettingsthatyoumaywishtochange(NOTE:afteranychangeismade,youmusthit“Apply”).Thissectioninthismanualisnotall-encompassing;forfurtherdetailsseetheonlineQualisysusermanualsection“Projectoptions”byclickingHelp>Helptopics.

InputDevices.Hereyoucanchangewhichdevicesyouwillbecollectingwith.Oquscamerascannotbedisabled,butinthissettingwindowyoucanselectordisableforceplatecollection(USB-2533)andEMGinputs(MEGA,Noraxon).


Markercapturefrequency.Changethisnumbertoadjusthowmanyframesofmarkerdataarecollectedpersecond.Capturefrequencyneededdependsonspeedofmovementbeingcaptured.

Timingoptions.Usethesesettingstoactivateanexternaltrigger.Thismaybedesiredifyouaresynchronizingmultipledataacquisitionsoftwares(e.g.,QTMandEMGsoftware)orifyouwishtotriggeradatacapturefrominthecollectionspaceinsteadoffromthecomputer.


Camerasettings.Inthistabyoucanadjustcapturesettingsforindividualcameras.NotethatyoucanadjustsettingsformultiplecamerasatatimebyholdingCtrlwhileclickingoncameranumbersoryoucanadjusteachcameraindividually.Ingeneral,camerasettingsshouldnotneedtobechanged.Instanceswhentheymayneedtobeadjustedincludetheuseofadeviceinthecapturespacethatisveryshinyorverysmallorverylargemarkers.IfyouwishtochangecamerasettingsitisrecommendedthatyoureadtheQualisyshelptopics.


Analogsettings.Hereyoucanchangethecapturerateforanyanalogdevicesyouincludeinyourproject.Sampleratesmustbeamultipleofthecameraframerateandcanbechangedintheboxoutlinedbelow.Therearealsosettingshereforremovingoffsetfromanalogdata(yellowbox).Usecautionifactivatingtheseoptionsasthesoftwareoffsetremovalassumesthatthereisnothingontheforceplateswhenitremovesanybaselineoffset,afeaturethatmaybeproblematicifsomeonehappenstocrossoveraforceplateduringtheoffsetremoval.


3Dtracking.Hereyoucanadjustsettingsforerrortolerancein3Dmarkertracking.

“Predictionerror”isthemaximumdistancebetweenamarker’spredicted locationandthecapturedlocationofthemarker.Ifthecapturedlocationis outsideofthispredictedlocation,thesoftwarewillnotreconstructit.Increasethis valuetoincreasethetoleranceinreconstruction.

“Maximumresidual”isthemaximumdeviationincamera2Dviewsofamarkerthatcanbeconvergedintoasinglemarkertrajectory.Qualisysrecommendsthatthisvaluebe2-5timestheaverageresidualvaluesfromthecalibrationresults.

“Minimumtrajectorylength”istheminimumnumberofframesofdatato createatrajectory.Dataseenforfewerframeswillnotbeassignedtoatrajectory. Theminimumvalueforthisoptionis2.

“Autojoin”automaticallyjoinstrajectoriesthatappeartobelongtothesame markerin3Dspace.Thismayreducethenumberofpartsinatrajectoryandmay maketrackingeasier.Thisdoesnotautomaticallyfillgaps.

Trajectories.Hereyoucanadjustsettingsforautomaticallyfillinggaps.


ForceData.Inthesesettingsyoucanselectwhichforceplatesyou’dliketocollectwithandcheckadvancedforceplateoptions.

GUI.InGUI,2DViewSettings,and3DViewSettingsyoucanadjustsettingssuchasthereal-timescreenrefresh,andtheappearanceofthescreenin2Dand3Dmodes.

Miscellaneous.HereyoucanadjustfoldersettingsandQTMstartupsettings.Pleasedo notadjustthe“Globaldirectories”options(inFolderOptions)orthe“Automaticallyloadaprojectatstartup”options(inStartup)asthesechangedefaultsforallusers.

ProjectoptionsareautomaticallysavedtoyourprojectSettingsfilewhenyouclick“Apply”andthen“OK.”Itisrecommendedthatyoualwayspilotdatacollection,processing,andanalysisusingyournewprojectsettings.Thiswillensurethatyoursettingsaresufficienttocapturetheinformationyouneed.


Chapter1.2

QTMDataCollection

Ifcollectingdataonaparticipant,turningthesystemonandcalibrationstepsshouldbeperformedbeforetheparticipantarrives.Camerasandforceplatesshouldbeallowedtowarmupforatleast20minutesbeforecalibrating.Turningthingson:

1.Turnondatacollectioncomputer.

2.Turnonpowerstripforcameras(locatedontowertoleftofdatacollectioncomputer)

3.Makesureforceplatesareon(3boxesdirectlytotheleftofthecomputermonitors).

4.Find/addnewparticipantfoldertoprojectfolder(C:\Users\Qsys02\Documents\yourprojectfolder\Data).

5.OpenQTM

Powerswitch

Manualzeroingbutton


6.Loadyourproject

7.Ifyouneedtocreateanewproject,followinstructionsinChapterX.X


Calibration:

1.Hitthe“new”button (topleftoftoolbar)

2.Clickon2Dview,checkcameraviewstomakesureeachcameraisseeing0markers.

3.PlaceLframeonforceplate1(endsmallFP)withthelongarmpointingtowardsthebigforceplate.Makesureitfitstightlytotheforceplatecorner(usedrop-downlegs),alignswiththeforceplateedges,andislevel.IfthelevelsareoffthetiltoftheLcanbeadjustedusingtheroundscrewsonthearms.AdjustthemuntiltheLislevelinbothplanes.


4.Putthecalibrationwandtogether.Clickthecalibrationbutton .

5.Adjustcalibrationsettingsaspreferred.Forcalibratingavolumeforgait(all3forceplates+space)60secondsmaybenecessary.Usingacalibrationdelaywillgiveyoutimetohit“OK”andgetsetinthevolumebeforethestartofcalibrationcapture.Whenyouarereadytocalibrate,hit“OK.”

6.Calibratethevolume.It’sgenerallyrecommendedtodothisinasystematicmethod,coveringtheentiretyofthedesiredcapturevolumewiththecalibrationwandinawaythatallcamerasviewthewandoverthefullvolume(i.e.,don’thaveyourbacktoonewallofcamerasthroughouttheentirecalibration).


7.Calibrationwillendwhenthesettimehaselapsed.Thiswindowwillpopup:

Standarddeviationofwandlengthshouldbelessthan1mm.Ifthiscriteriaismet,hit“OK”andputawaycalibrationequipment.

8.Calibrationwilloccasionallyfail.Ifcalibrationfails,checkcamera2DviewstomakesurenomarkersotherthantheLandthecalibrationwandarevisible.Ifnoothermarkersorreflectionsarevisible,startcalibrationoveragain.

Note:cameraswillpickupanythingreflective,includingrunningshoesandreflectivetagsonclothing.Makesureyoudonothaveanythingreflectiveonwhencalibrating.


DataCollection:

1.Withnothingontheforceplates,zeroforceplates(orangebuttonsonforceplateboxes).Whenplatesarezeroed,theorangelightsshouldbeoff.

2.Clickthe“capture”button. Thiswindowwillpopup:

3.AdjusttheCapturePeriodtothedesiredlengthoftime.Changingthetimeboxwillautomaticallychangethenumberofmarkerframesbox.

4.Checkthe“Savecapturedandprocessedmeasurementautomatically”box.Makesurefolderthatisdirectedtoiswhereyouwouldlikeyourdatatobestored.

5.Changethe“Name”boxtowhateveryouwouldliketheprefixofyourfilestobe.Ifyouwouldlikefilestobeautomaticallynumberedasyoucollectthem,check“Addcounterstarting…”

6.Click“Options…”tocheckCameraSystemSettingstomakesureframerateandtriggersettingsarecorrect.


7.Whenyouarereadytocollectatrial,hit“Start.”

8.Attheendofthecaptureperiod,thetrialwillopenin3Dview.Playthroughthetrialusingthebuttonsinthe

toolbar:

orbydraggingthetoparrowalongthebottomtimebar:

9.Labelingdata(tocreateanAIMmodel,useamotiontrial,notastatictrial):

Scanthroughthetrialtomakesureallnecessarymarkerandforcedatawerecaptured.Movedatatoatimepointwhereallmarkersarevisibleinthe3Dview.

Tolabeldata,firstopentrajectorywindowsbyclickingView>TrajectoryInfo>All.

Right-clickinthe“Labeledtrajectories”windowandselect“Loadlabellist…”

Selectyourmarkerlist.

Ifyouhavenotpreviouslycreatedalabellist,right-clickontheword“Trajectory”andselect“Addnewlabel.”Thiswillcreatea“New####”label.

Addasmanylabelsasmarkersinyourmarkersetandnamethemappropriately.

Whenalllabelsarecreated,right-clickinthe“Labeledtrajectories”windowandselect“Savelabellist…”

II.Tobeginlabelingmarkers,selectthefirstmarkerinthelabellistandclickthe

button(leftverticaltoolbar).

III.Inthe3Dview,clickonthemarkercorrespondingtothefirstlabel.InID mode,theprogramwillprogresstothenextlabelinthelabellistwhenamarkerisclickedon.

IV.Continueclickingonthemarkersinthe3Dviewthatcorrespondtothelabel highlightedinthelabellistuntilyoureachtheendofthelabellist.

V.Clickthe buttontoturnoffIDmode.

C.Trimtrialsothatonlylabeleddataareincludedusingthesidearrowsofthetimebar.Onlylabeleddataareincludedwhenallmarkerfilllevelsareat100%(inlabeledtrajectorywindow).

D.Click“GenerateAIMmodel”button


E.Select“Createnewmodel”inthewindowthatpopsupandthenclick“Next.”

F.Inthenextwindow,verifythatthemodellookscorrect,andthenclick“Next.”

G.EnterafilenameforyourAIMmodelandclick“OK.”Inthefinalwindow,click“Finish.”

10.TohaveAIMappliedasyoucollectdata,inProjectOptions,gotoAIMandaddthemodelyoujustcreatedtothe“Appliedmodels:”window.Hit“Apply,”then“OK.”

11.Continuecollectingtrialsuntilfinished.

12.Ifyouwishtooverwriteatrial,settheNameandcountertomatchthetrialyouwishtooverwrite.Whenyouhit“Start,”awindowwillpopupaskingifyouwishtooverwritethetrial.Datawillbegincollectingwhenyouhit“Yes.”

13.Whendatacollectioniscompleted,turnoffcamerasandcopydatatoappropriatelocation(e.g.,externalharddrive,networkfolder,etc.).


Chapter1.3-QTMDataProcessing

DataProcessinginQTM

Afterdataarecollected,theymustbetrackedandexportedfromQTMforfurtheranalysis.

Trackingmarkerdata

1.Navigatetolocationofmotioncapturedataonthecomputer.QTMfilesendin*.qtmandcanbeopenedonacomputerwithQTMsoftwareeitherbydouble-clickingonthefilefromWindowsExplorerorusingthe

“Openfile” buttoninQTM.

2.Beforetrackingyourdata,youmaywishtotrimthetrialtoonlytherangeofframesofinterest.Forexample,youmaywishtodothisifyouwantasinglestrideofdatafromatrialwhichincludesdataofyourparticipantwalkingallthewayacrossthedatacapturespace.

A.Toshortenatrial,clickanddragthearrowbuttonsoneitherendofthetimebar.

B.Forexample,hereIhavetrimmedoffthebeginningandendofatrialofdata.

C.Onceyoutrimatrial,markerinformationwillonlybedisplayedfortheframesinside thetimebararrows.Notethatthisrangecanalwaysbechangedlateronifyoudecideyou wanttotrackmoreofyourtrial.

3.Ifmarkersarealreadylabeled,skiptostep5.


4.Ifmarkersarenotyetlabeled,alabellistwillneedtobecreated.Un-labeledmarkersappearredandalltrajectorieswillbelistedinthe“unidentifiedtrajectories”box.Ifyoudon’tseethetrajectoryboxes,clickView>TrajectoryInfo>Alltoopentheseboxes.

A.Right-clickinthe“Labeledtrajectories”windowandselect“Loadlabellist…” Selectyourmarkerlist.

I.Ifyouhavenotpreviouslycreatedalabellist,right-clickandselect “Addnewlabel.”Thiswillcreatea“New####”label.

II.Addasmanylabelsasmarkersinyourmarkersetandnamethem appropriately.

III.Whenalllabelsarecreated,right-clickinthe“Labeledtrajectories” windowandselect“Savelabellist…”


B.Tobeginlabelingmarkers,selectthefirstmarkerinthelabellistandclickthe

button.

C.Inthe3Dview,clickonthemarkercorrespondingtothefirstlabel.InID mode,theprogramwillprogresstothenextlabelinthelabellistwhenamarkeris clickedon.

D.Continueclickingonthemarkersinthe3Dviewthatcorrespondtothelabel highlightedinthelabellistuntilyoureachtheendofthelabellist.

E.Clickthe buttontoturnoffIDmode.

F.Trimtrialsothatonlylabeleddataareincludedusingthebottomarrowofthetimebar:

G.Click“GenerateAIMmodel”button

E.Select“Createnewmodel”inthewindowthatpopsupandthenclick“Next.”

F.Inthenextwindow,verifythatthemodellookscorrect,andthenclick“Next.”

G.EnterafilenameforyourAIMmodelandclick“OK.”Inthefinalwindow,click “Finish.”

H.AftercreatinganAIMmodel,themodelcanbeappliedtonew,unlabeledfilesofthesame

participant/datacollectionsessionusingthe button.


5.Ifmarkersarealreadylabeled,thenextstepistoclean-upthedata.Generally,thegoalofcleaning-upthedataistomakesuretherearenogapsinmarkerdata.

Note:itisrarethattherearenomarkersin“Unidentifiedtrajectories,”evenwhenallmarkersarelabeled.


6.First,checkthatallmarkersarecorrectlylabeled(e.g.,theRightASISisactuallylabeledasRASIratherthanasRSHForsomethingelse).Tocheckthis,youcanclickonamarkerinthe“Labeledtrajectories”boxanditwillbehighlightedinthe3Dview(andviceversa).Alsonotethatlabelcolorsinthe“Labeledtrajectories”boxmatchthecolorofthemarkersinthe3Dview.

A.The“FillLevel”columnforeachmarkertellsyouwhatpercentofthetrialcontainsdataandalabelforthismarker.The“Range”columntellsyouduringwhichframesthelabeledmarkerappears.Attheendofdatatrackingmarkerdatayouwantallmarkersofinteresttobeat100%fortherangeofframesofinterest.

B.The+signnexttoamarkerindicatesthatthismarkertrajectoryismadeupofmultiple parts.Ifyouexpandbyclickingonthe+sign,youcanseehowmanypartsareinagiventrajectoryandwhatframestheyappearduring.


7.Whenyouhaveconfirmedthatallmarkersarelabeledcorrectly,youcanbeginfixinggapsindata.Thereare2generalmethodsforfixinggapsindatawhicharedetailedbelow.

A.Option1:fillingagapinamarkertrajectorywhenthereisanunlabeledtrajectory available(bestoptionifavailable)

I.SometimesQTMdoesnotdoaperfectjobofidentifyingwhichmarkeris which,evenafteryoulabeldata.Thiswillresultinsectionsofdatawherethereis amarkerinthe3Dviewbutitisunlabeled(orinsomecases,islabeled incorrectly).Below,weseethattheLeftPSIS(LPSI)islabeledfor90%ofthe trial,exceptfortheframerange1960-2559.Thereisanunidentified trajectorythatcoincideswithmostofthisframerange.

II.ToaddthisunidentifiedtrajectorytotheLPSI,clickandholdoneitherthe unidentifiedmarkerinthe3DviewortheUnidentifiedtrajectoryinthe “Unidentifiedtrajectories”box,anddragittoLPSIinthe“Labeledtrajectories” box.


III.NowtheunidentifiedtrajectoryhasbeenaddedtoLPSI.

IV.Sometimestheunidentifiedtrajectoryyouaretryingtoaddtofillagapina labeledmarkeroverlapswithexistingdataandyouwillgetthefollowing message:

a.Ifyouhit“OK,”aboxshowing3Dtrajectorieswillpopupinwhich youcanselecttherangeoftheunidentifiedmarkeryou’dliketokeep. Thistoolcanbeunwieldyanddifficulttouseattimes.

b.Ifyouhit“Cancel,”youcaninsteadmanuallyidentifytheoverlapping data.


c.Intheexamplebelow,youcanseethattheunidentifiedtrajectorycontains2partsofdatawhichcovertherange1961-2558.LPSIalreadyhasdataforframes2553-2559.Todeterminewhichdatatokeep(thedataalreadyinLPSIfortheseframesorthedatafromtheunidentifiedtrajectoryfortheseframes),navigatetothisframerangeinthe3Dviewusingthetimebaratthebottomofthescreen.

d.Duringtheoverlappingframes,youshouldbeabletoseetwomarkers. Thesemayoverlapverycloselyinspace(asshownbelow)ortheymay not.

e.Atthispointyouneedtochoosewhichdatayouwanttokeep.Ifyouwishtokeepsectionsofonemarkerwhichgobeforeandafteranother(inthiscase,saywewanttotaketheunidentifiedmarkerfortherangebeforeandaftertheoverlappingLPSIdata),youmayneedtosplitonetrajectory.Todothis,navigatetothelasttimepointyou’dliketokeepofthetrajectoryofinterest,right-clickonthetrajectoryinthe“Labeledtrajectories”or“Unidentifiedtrajectories”box,andclick“Splitpartaftercurrentframe.”Thiswillcuttheselectedtrajectoryinto2parts.Youmayneedtodothisagainattheendoftherangeofinterest.Thenthesplitpartorpartscanbedraggedandaddedtothelabeledtrajectory.

B.Option2:gap-fillingagapinamarkerwhenthereisnounidentifiedtrajectorytoswapin

I.Below,weseeanexampleofaframeofdatawherethereisnoLPSI,andnounlabeledtrajectorytoswapintoitsplace.Incaseslikethis,youhavetoperformagapfill.


II.Togapfilldata,selectthePartsoneithersideofthegap(below,therearedata from1-1959and1961-2552,butnodataforframe1960),right-click,andselect “Gapfilltrajectorywithpreview”


III.Awindowdisplayingthe3Dtrajectoryoftheselectedmarkerwillpopupandshowhowthegapistobefilled.Followingtheinstructionsinthebottomofthiswindow,youcanzoominonthegapandadjusttherangeofframesyouwantthegapfilltospan.Notethatgapfillsmustbeginandendatlabeledmarkerdataasthefillisconstructedusingapolynomialfitbasedonexistingdata.

Gaprangeintrajectories(X,Y,andZ)

Rangeofgapbeingshown


IV.Generalguidelinesforgapfillingdata:

a.Usecautionwhenfillinglargergaps(rangedependsonfrequencyof datacapture,speedofmovement,etc.).Gapfilleddataisnotrealdataand largegapfillsmaybeadistortedrepresentationofthetruemotionofa marker.

b.Adjustgapfillframessothattherearenotabruptshifts/discontinuitiesinthe3Dtrajectories.Oftentimesbeforeamarkerdropsoutandcreatesagap,thecamerasarehavingdifficultycalculatingitspositionandsothelast/firstframeortwoofexistingdatawillbeshifted.

c.Foranygapfilllargerthanafewframes,alwaysviewthe3Drepresentationofthegapfillbyplayingthroughthecorrespondingframeswhilewatchingthe3Dviewofyourdatatocheckforvisibleerrorsinthegapfilleddata.

8.Whenallmarkersarelabeledat100%,datatrackingiscomplete.

9.Afterdatatracking,datamustbeexportedfromQTMforfurtherprocessing.Filescanbeexportedinthefollowingformats:

-C3D:Visual3Dfileformat

-TSV:tab-delimiteddatathatcanbeviewedinexcelandreadintoavarietyof programs

-MAT:MatLabfileformat

-AVI:videofileofmarkerdata

A.Filescanbeexportedone-at-a-timeorinbulk.

I.Toexportfilesinbulk,clickFile>BatchProcess

a.WhenusingBatchProcess,thefirstwindowtopopupasksyouto selectthedesiredfiles.Afterselectingthedesiredfilesandclicking “Open,”awindowsimilartotheonebelowwillpopup.


b.Inthe“Processing”tab(shownbelow),youcanselectwhichfileformatyouwanttoexporttoandalsosetadditionalprocessingoptions.Ifyouwanttoexportthedataintheformthatyoucurrentlyhave(e.g.,markerstracked,trialstrimmed,etc.),DONOTselectanyoptionsexceptforyourexportingfileformat.Otheroptionsmayundoworkthatyouhavealreadydone(e.g.,selecting“Trackthemeasurement”willre-trackallfilesinraw,unlabeledformat).

c.InthesecondaryBatchProcessingtab(above,thiswouldbethe“C3DExport”tabonthetopleft),youcanselectoptionsspecifictothatfileformat.

II.Toexportfilesone-at-a-time,clickFile>Export>[desiredfiletype].Using thisdialog,onlythesecondarywindowoptionsdescribedaboveinCwillpopup.

10.Afterexportingdata,processinginQTMiscomplete


Chapter1.4-ExampleMarkerSets

TheHumanMotionCorehassomestandardmarkersetsthatmaybeusedformotioncaptureof

certaintasks.Thesemarkersetsincludefull-body,rightlowerextremity,leftlowerextremity,andheadwithFrankfortplane.Userscanfeelfreetocopythesemarkersetsandmodifyasneeded.Ingeneral,thefullbodyandlowerextremitymarkersetsareoptimizedforcalculationoflowerextremityjointmechanics.Amoredetailedmarkersetthanthefullbodymarkersetwouldbeneededtocalculateupperextremitymechanics,butthecurrentmarkersetcanbeusedtoaccountforsegmentsizes/massesforcenter-of-masscalculations.

Belowareimagesofthestandardmarkersetswithmarkerslabeled.Attheendofthisdocumentisadefinitionofthemarkerlabels.Notethatinthestandardmarkersets,markerlabelsareconsistentfrommarkersettomarkerset.


UnilateralLowerExtremityThismarkersetexistsinarightsideandleftsideversion.Therightsideisshownbelow.Therearesomedifferencesinthemarkersonthelowerextremitybetweenthismodelandthefull-bodymodel.Thismodelhasafootmarkersetthatcanbeusedtocalculatewholefootandrearfootangles.Additionally,thismodelhasdifferentpelvicmarkerscomparedtothewholebodymodelwhichwillleadtodifferencepelvicsegmentdefinitionsbutnosubstantialdifferencesinsegmentmotioncalculations.

RASI LASI

LGRT RGRT

LPSI RPSI

RMCO RLCO

RMMA RLMA

R1MH R5MH

RTOE

RTHF RTHU

RTHL RTHR

RSHF RSHU

RSHL RSHR

RHLA

RHPR RTOE

RPSI RASI

RGRT

RHPR

RHLA RHMD

RMMA RLMA

R5MH

RTOE

Frontalviewofunilateralmarkerset

Closeupofrearfootofunilateralmarkerset

Posteriorviewofunilateralmarkerset


RASI LASI LILC RILC

SLJ

RACJ LACJ

LLCO LMCO

RMCO RLCO

RMMA RLMA LLMA LMMA

L5MH L1MH LTOE RTOE

R1MH R5MH

FLH FRH

BRH BLH

TOPH

LELB RELB

RDUL

RDRA LDRA

LDUL LBTH

LHAN

LFIN RFIN

RHAN

RBTH

FullBody

Thismarkersetincludesthesamegenerallowerextremitymodelasthelowerextremitymarkersets,butaddsatrunk,arms,andahead.Notethatthismarkersetdoesnotincludethe3-markerrearfoot.Thiscanbeeasilyaddedasshowninthelower-extremityimage.

Frontalviewoffullbodymarkerset


Viewofright/frontsideoffull-bodymarkerset

LTHF

LTHL

LTHU

LTHR

RTHF

RTHL

RTHU

RTHR

LSHF

LSHL

LSHU

LSHR RSHF

RSHL

RSHU

RSHR


PosteriorViewoffull-bodymarkerset

RARF RARU

RARL RARR

RFAF

RFAL RFAU

RFAR LFAR LFAL LFAF

LARU

LFAU

LARF

LARR LARL

BLH

RACJ

SLJ

RHEEL LHEEL

LACJ

BRH

TOPH


HeadMarkersetforGazeTracking

FLH FRH

BRH BLH

TOPH

LAUM

RZYA LZYA

RAUM LAUM RZYA

LZYA

RAUM

TOPH

BLH FLH

FRH


MarkernamedefinitionsMarkerlabelsarelistedbysegment

Head:Crown/helmetTOPH–topofheadFRH–frontrightofheadBRH–backrightofheadFLH–frontleftofheadBLH–backleftofheadFrankfortplaneR/LAUM–right/leftauditorymeatusR/LZYA–right/leftzygomaticarchTrunk:R/LACJ–right/leftacromioclavicularjointUpperBackTrunkClusterLowerBackCluster(ifyouwantbothlumbar&thoracicspine)Arm:UpperarmrigidclusterR/LARU–right/leftarmupperR/LARF–right/leftarmfrontR/LARL–right/leftarmlowerF/LARR–right/leftarmrearForearmrigidclusterR/LFAU–right/leftforearmupperR/LFAF–right/leftforearmfrontR/LFAL–right/leftforearmlowerF/LFAR–right/leftforearmrearR/LDRA–right/leftdistalradius(radialstyloid)R/LDUL–right/leftdistalulna(ulnarstyloid)R/LHAN–right/lefthandR/LBTH–right/leftbaseofthumbR/LFIN–right/leftsecondfingerPelvis:R/LASI–right/leftanteriorsuperioriliacspine(ASIS)R/LILC–right/leftiliaccrestR/LPSI–right/leftposteriorsuperioriliacspine(PSIS)SLJ–sacrolumbarjointR/LGRT–right/leftgreatertrochanterLowerextremity:Thighrigidcluster


R/LTHU–right/leftthighupperR/LTHF–right/leftthighfrontR/LTHL–right/leftthighlowerR/LTHR–right/leftthighrearShankrigidclusterR/LSHU–right/leftshankupperR/LSHF–right/leftshankfrontR/LSHL–right/leftshanklowerR/LSHR–right/leftshankrearR/LLCO–right/leftlateralfemoralepicondyleR/LMCO–right/leftmedialfemoralepicondyleR/LLMA–right/leftlateralmalleolusR/LMMA–right/leftmedialmalleolusR/L1MH–right/leftfirstmetatarsalphalangealjointR/L5MH–right/leftfifthmetatarsalphalangealjointR/LTOE–right/lefttoeR/LHEEL–right/leftheelR/LHPR–right/leftheelproximalR/LHMD–right/leftheelmedialR/LHLA–right/leftheellateral

CalibrationMarkersBothCalibration&TrackingMarkersTrackingMarkers


CHAPTER1.5-Visual3DTutorial

Visual3D(V3D)isamotioncaptureanalysissoftwarepackagethatcanmakedataanalysisrelativelystraightforwardandstreamlined.C-Motion,thedevelopersofV3D,havecreatedahigh-qualitysetofdocumentationforthissoftware(https://www.c-motion.com/v3dwiki/index.php/Visual3D_Overview).Ifyouareafirst-timeuserofV3DitishighlyrecommendedthatyougothroughtheV3Dtutorials(https://www.c-motion.com/v3dwiki/index.php/Visual3D_Tutorials)tofullyappreciatethetoolsincludedinthisprogram,aswellastheimplicationsofincorrectlymodeling/computingyourresults.

I.V3DBasics

1.V3Dworkswithmotionfilesthatarein.c3dformat.MotioncapturedatacanbeexportedfromQTMinthisformatwhichwillincludeanymarker,analog,andforcedatacapturedinatrial.NOTE:V3Dcannotworkwithfiles/commands/tagsthathavespacesinthename.Neverusespacesinfilenames,markernames,etc.

2.ToloadrawdatafilesinV3D,clicktheopenbutton andselectthec3dfilesyouwishtoview.Whenfilesareloaded,theywillappearinthe“Workspace”tabinV3D.


3.Toviewthesedatafiles,switchtothe“SignalsandEvents”tab.Hereyoucanselectwhichfiletoviewusingthedrop-downmenu,playorscrollthroughatrialusingthetoolsatthebottom,orviewrawTARGET(marker),ANALOG,orFORCEdata.

Drop-downboxtoviewtrials

Playbackcontrols

ORIGINAL(raw)targetdata

3Ddataview


4.Ifyouleft-clickonanydataitem,aDataViewwindowwillpopuptoshowyoutrajectoriesandvaluesforthatdataitem.Youcanalsoright-clickonadataitemtoplotit.

5.Viewingrawdatahaslimitedutility.Generallyyouwillwanttobuildamodelandapplythismodelyourdatatrials.

II.ModelsinV3D

ModelsinV3Darethebasefilesfromwhichskeleton/segmentcharacteristicsareappliedtomotionfiles.Inordertocreateamodeltoapplytoyourmotionfiles,youmusthaveastaticcalibrationtrial.Forhumanmotion,especiallygait,thestatictrialisgenerallycapturedwhileanindividualstandsstillinanatomicposition.Theseinstructionswilltakeyouthroughsomegeneralstepsforcreatingamodelfromastatictrial.Thisisnottheonlywaytocreateamodel;furtherinstructionscanbefoundontheV3Dwiki.

TherearedemoV3Dfilesthatyoucanusetoexplorethefollowinginstructions.AcompletedanalysiscanbeviewedbyopeningR_LE_walk.cmo.Thefilesusedtobuildthisanalysisincludeonestatictrial(R_LE_static.c3d),twotreadmillwalkingtrials(R_LE_walk01.c3dandR_LE_walk02.c3d)amodelfile(R_LE_model.mdh),andapipeline(R_LE_walk_pipeline.v3s).ThesefilesarefordemonstrationonlyandcanbeusedtoexploresomecapabilitiesofV3DbutarenotinclusiveofalltasksthatV3Discapableof.

Therearesomepre-createdmodelsavailableforgeneraluse.Theseincludeunilaterallowerextremitymodelsforboththerightandleftlimbsandafull-bodymodel.Thesemodelsarebasedonthemarkersetsthatarealsoavailableforgeneraluseandcanbeusedandmodifiedasneeded.Thesemodelsarenotmeanttobeall-inclusivebutratherprovideexamplesandstartingpointsfordevelopmentofproject-specificmodels.NoteespeciallythattheupperextremitiesinthefullbodymodeldonotfollowISB

X,Y,Ztrajectory

Dataresidual

Dataidentity


recommendationsfortheshoulder,elbow,orforearm.UsersshouldalwaysreviewtheV3Dwikirecommendationsformodelingofsegmentsandjointsabovethepelvis.

Generalfactsaboutmodels

ModelsinV3Dconsistofdefinitionsofsegmentsandlandmarksusedtocreatethesesegments.Thesedefinitionsareneededsothatthe3Dorientationofsegmentscanbetrackedduringamovement.Ingeneral,segmentsaredefinedusingrealorvirtually-definedmarkersattheproximalanddistalends,aswellasradiioftheproximalanddistalends.Atleastthreemarkersareneededtodefinetheorientationofasegment.Whenasegmentiscreated,itisassignedacoordinatesystem(seered,green,andblueorthogonalaxesinimagebelow)whichisrigidlyassociatedwiththesegment’sproximaljoint(e.g.,thethighcoordinatesystemwillbeanchoredatthehipjoint).Modelsmustalsoincludeassignmentoftrackingmarkers,ormarkersthatareregisteredtothesegmentdefinitioninacalibrationtrialandarethenusedtotrackthemovementofthesegmentduringmovementtrials.Thesegmentcoordinatesystemismodeledasrigidlyrelatedtotheorientationofthetrackingmarkersandforthisreasontrackingmarkersareoftenplacedonplasticshells(e.g.,thighandshankclusters)oronanatomicallocationswithlittleskinmotionartifact(e.g.,acromio-clavicularjointsduringlowerextremitytasks).Inadditiontosegment-specificdefinitions,modelsrequireanindividual’sheightandbodymassforcalculationofsegmentinertialpropertiesandjointkinetics.

Theorientationofadjacentsegmentcoordinatesystemswithrespecttoeachotherprovidesthejointangleorjointtranslationalposition.Forthisreason,itisimportanttopayattentiontotheorientationofsegmentcoordinatesystemsastheyarecreated.Intheimagebelow,youcanseethatallvisiblesegmentshavethered(X)axisorientedtothemodel’sleft.Thismatchingorientationprovideseaseofunderstandingforjointdefinitions.Forexample,rotationsaboutXbetweentheshankandthigharesagittalplanejointangles,thoseaboutYarefrontalplanejointangles,andthoseaboutZaretransverseplanejointangles.Inordertoobtainaccurateandmeaningfuldata,itiscriticalthatyoucorrectlydefineandconstructyourmodel.RecommendationsformodelingsegmentsandjointsofinterestcanbefoundontheV3Dwiki,aswellasinpublishedrecommendationsfromtheInternationalSocietyofBiomechanics.

Applyinganexistingmodeltomovementtrials

InanewV3Dworkspaceorwindow,onthemenu,click“Model,”then“Create(AddStaticCalibrationTrial),”and“HybridModelfromC3DFile.”

A.Inthewindowthatpopsup,selectyourstaticcalibrationtrial.

B.V3Dwillautomaticallyswitchtothe“Models”tab.

C.ClicktheLoadModelTemplatebutton

i.Selectthemodelfile(*.mdh)youwouldliketouse.

ii.Themodelshouldnowbeappliedtoyourstatictrial.


iii.Insomecases,therewillbeerrorsinbuildingthemodel.Thesewillbedetailedina“BuildModelResults”window.


iv.Ifthisoccurs,youcanscrollthroughtheerrorsand/orwarningstodetermine whatissuesarepresent.Fixingtheseissuesmayinvolvefindingacorrect, completestatictrial,correctingthenamesofmarkers,ormodifyingthemodelto correspondtodifferentsegment/landmarkdefinitions.

D.Definitionsofhowmodelsegmentsandlandmarksarecreatedcanbefoundbydouble-clickingonasegmentorlandmarkintheleft-handwindowintheModelstab.


i.Forexample,ifyoulookattherightthighinthismodel,youwillseethefollowingdefinitions.

ii.Notethatsomeofthemarkersusedfordefiningtheproximalanddistaljointsarerealmarkersthatwereplacedonanindividual(e.g.,RLCO,RMCO)whileothermarkersare“landmarks,”orvirtualmarkerscreatedinV3Dbasedonthepositionofothermarkers.Here,theF_RHIPisalandmark.


iii.IfwelookatF_RHIPinthe“Landmarks”tab,wecanseehowthislandmarkwascreated.Thislandmarkisahipjointcenterdefinitionbasedonliteratureregressionequations.

E.Afterapplyingyourmodel,theworkspaceshouldlooksomethinglikethis:


F.Nowyoumustaddmotionfiles.Todothis,clicktheopenbutton andselect yourmotiontrials.Awarningwindowwillpopup,select“Insertnewfilesintoyour currentlyopenworkspace.”

i.Afterselectingyourmotiontrials,yourworkspacewilllooksomethinglikethis:

G.Youcannowapplyyourmodeltoyourmotionfiles.Todothis,clickModel>Assign ModeltoMotionFiles.Selectthetrialswhichyouwouldliketoapplythemodelto


i.Nowyourworkspaceshouldshowyourmotionfileswithintheirassigned model.

ii.Ifyouviewyourmotiontrialsinthe“SignalsandEvents”tab,youwillseethe modelappliedtothetrials(assumingyourdataweretrackedcorrectlywith markernamesthemodelexpected.)Notethatthemodelisnotnecessarilytoscale orcompletelycorrectanatomically(e.g.,modelfemoralheadsareoftennotinthe acetabulum),themodelismeantasavisualaid.

iii.Ifasegmentismissingorincorrectlyorientedinamotiontrialitisdueto incorrectlylabeleddata.Checkthatalltrackingmarkersarepresentinthemotion trialandthatthemarkernamesassignedtothemotiontrialmatchthoseinthe model.


III.DataAnalysisandPipelines

OnceyouhavedataloadedintoV3Dandamodelassignedtoyourmotionfiles,youcanprocessyoursignals,createmotionevents,andcomputeoutcomes(e.g.,jointkinematicsandkinetics).Thiscanbedoneusingsomepoint-and-clickstepsbutismoreefficientlyimplementedusingpipelines.Pipelinesconsistofaseriesofcommandswhichcanbesavedandusedformultipleparticipants/conditions/iterationsinastudyandthusassistwithbatchprocessingandcalculationconsistency.Thefollowinginstructionswillgothroughsomebasicpipelinecommandsthatmaybeusedduringanyanalysis.Asyouworkthroughpipelinecommandsitwillbecomeclearthattherearemanytoolsavailableinpipelines.FurtherinformationabouteachcommandcanbefoundintheV3Dwikidocumentation.Foranexamplepipeline,intheCPHMdemonstrationdataprovided,theanalysisoftheR_LE_walk.cmofilewasperformedusingtheR_LE_walk_pipeline.v3sfile.

1.Tousethepipelinetool,clickonthepipelinebutton. Thefollowingwindowwillpopup.

2.Inthelefthandpaneofthiswindowareallavailablecommands,organizedintosubgroups.Thecenterpaneshowscommandsinthecurrentpipeline,andtherighthandpaneshowsapreviewofacommandinthecurrentpipelinewhenitisselected.Forexample,ifyouweretostartcreatingapipelinewithacommandtocreateamodelfromastatictrial,thewindowwouldlooklikethis:


3.Whenyouhaveacommandinacurrentpipelinehighlighted,theinitialtextofthatcommandaswellasadescriptionofthecommandwillappearintherighthandpane.Toeditthecommand,double-clickonthecommandinthemiddlepane.Forthe“Create_Hybrid_Model”command,double-clickingonthecommandwillbringupawindowoftextthatcanbeedited.Inthesetypesofwindows,“!”indicatesthatthepipelineshouldskipthislineofcodeorusethedefaultvalue.Inthewindowbelow,thismeansthatthereisnospecificfilethatthepipelineispulling,itwillinsteadopenadialogwindowaskingtheuserto“Openstandingfile.”Ifthereisaspecificfileyou’dliketoloadusingthiscommand,thepathtothisfilecanbeenteredinthe“/CALIBRATION_FILE=”lineandthe“!”canberemovedfromthisline.

Textofcommand

Descriptionofcommand


4.Insomecases,therearetwodifferentformatsinwhichtoeditacommand.Forexamplewhenyoudouble-clickonthe“Event_Copy”command,itopensaGUI-typewindow.Ifyouprefertoeditthecommandintext

format,youcanclickonthecommandinthemiddlepaneofthepipelinewindowandclickthe button.


5.Whenapipelineiscreatedandsaved,the.v3sfilecanbeopenedasatextdocumentforeaseofbatchediting(e.g.,changingexportfilenamesonasubject-by-subjectbasis).

6.Pipelinewindowbuttons:

A.“OpenPipeline…”allowsyoutoopenapreviously-createdpipeline.Ifyouclickthis buttonwhencommandsarealreadyinthemiddlepaneofthepipelinewindow,youwill begiventheoptiontoreplacethecurrentcommandsorappendthepipelineyouare openingtotheendofthecurrentcommands.

B.“SavePipelineAs…”allowsyoutosaveyourpipeline.Pipelinesdonotautomatically save.Ifyouclearthepipelineorreplacethepipelinebyopeninganotherpipeline,the pipelinewillnotbesavedandyouwillnotbepromptedtosaveit.

C.“ClearPipeline”clearsallcommandsfromthemiddlepaneofthepipelinewindow.


D.“ClearParameters”willclearanyparametersenteredinpipelinecommands.

E.“Step”willexecutethecommandcurrentlyhighlightedinthemiddlepane.

F.“ExecutePipeline”willexecutetheentiretyofthecommandsinthemiddlepaneofthe pipelinewindowfromthefirstcommandtothelast.

G.Notethatthereisno“undo”buttonanywhereinV3D.

7.Keypipelinecommands.Userscanfollowalonginthe“R_LE_walk_pipeline”forexamplesoftheimplementationofeachofthesecommands.

A.Create_Hybrid_Model+Apply_Model_Template:whilemodelscanbecreatedandappliedusingthepointandclickstepsdetailedabove,itisoftenusefultoautomatethesestepsonceamodelhasbeencreatedandpiloted.The“Create_Hybrid_Model”commandwillpromptausertoopenastandingcalibrationtrialandloadthistrialintotheV3Dworkspace.The“Apply_Model_Template”commandwillprompttheusertoselectamodel(.mdh)fileandthenapplythismodeltothestandingcalibrationtrial.

B.File_Open:thiscommandpromptstheusertoopenfileswhichwillbeloadedintothe V3Dworkspace.Inthe“R_LE_walk_pipeline,”thiscommandisusedtoloadmovement trialsafterthecreationofthemodel.

C.Assign_Model_File:thiscommandwillprompttheusertoassignthemodelfileto movementtrials.

D.Lowpass_Filter:target(marker)andanalogdataoftenneedtobefilteredtoremove noise.TherearemanyfilteroptionsinV3Dpipelines(seethe“SignalFilter”sectioninthelefthandpaneofthepipelinewindow).Ingeneral,rawdatashouldbefilteredbeforeperformingcalculations.Whichfiltersshouldbeusedandatwhatstepoftheanalysistheyshouldbeimplementeddependsonthetaskbeingperformedandtheanalysisrequired.Inthe“R_LE_walk_pipeline”example,alowpassButterworthfilterisappliedtothemarkerdatatoremovesignalabove8Hz.The“NUM_REFLECTED”lineinthecommandprovides100reflectedpointsoneithersideofthedatatominimizetimeshiftsinthedataduetothefilter.Notethatwhenthetargetdataarefiltered,thefiltereddataareplacedinanewfolder:TARGET>PROCESSED,whiletheoriginal,unfiltereddataremainintheTARGET>ORIGINALfolder.

E.Interpolate:thiscommandwillfilldatagapsofaspecifiedsize.Thisshouldnotbe usedinlieuoftrackingdatainQTMbutratherasafinalsweeptocorrectanysmallgaps thatweremissedintracking.

F.Select_Active_File:thiscommandcanbeusedtoselectallfilesorgroupsoffilesfor furtherpipelinecommands.Whenpairedwithanearlier“Assign_Tags_To_Files,”the Select_Active_Filecommandcanbeusedtoperformpipelinecommandsononlyasubsetoffiles.

G.Eventcommands(e.g.,Event_Minimum,Event_Maximum,Event_Threshold):canbeusedtoautomaticallycreateeventssuchasheel-strikes,toe-offs,maximumflexion,etc. Thesecommandscanbefoundinthe“EventCreation”and“EventManagement” sectionsofthelefthandpaneofthepipelinewindow.Notethatitisoftenmostusefulto editthesecommandsusingthe“Text”optionratherthanthedefaultGUIwindow.


H.Forloops:the“For_Each”and“End_For_Each”commandscanbeusedtobeginand endforloops.Inthe“R_LE_walk_pipeline”example,theseareusedtorecursively renamegaitcycleevents.

I.Signalmathcommands:thesecommandscanbeusedtoperformsimplearithmeticonsignals.Forexample,inthe“R_LE_walk_pipeline,”thenumberofframesbetweenconsecutiveheelstrikesandbetweenheelstrikesandtoe-offsareusedtodeterminegaitcycletimes(usingSubtract_Signals)and,subsequently,thepercentofthegaitcyclespentinthestancephase(usingDivid_Signals).

J.Metriccommands:thesecommandscreatenewsignalsinthe“METRIC”folder.

K.Compute_Model_Based_Data:thiscommandisperhapsthemostimportantcommandinV3Dpipelines.Withit,ausercancomputeahugerangeofvariables.Wheneditingthiscommandthefollowingwindowappears.Init,younamethedatavariableyouarecreating,identifywhattypeofvariableyouarecalculating,anddefinethedatabeing usedtocalculatethisnewvariable.TherearemanydifferenttypesofvariablesthatcanbecreatedandthespecificsofthealgorithmsusedorcomputationalordercanbefoundintheV3Donlinedocumentation.The“UseNegative”optionsallowyoutomultiplyasignalby-1asyoucalculateit,whichiscommonlyusedtoaligndatadirectionswithcommonreportingpractices(e.g.,theright-handrulewillgenerallyresultinkneeextensionbeingapositivevalue,whilereportingpracticesgenerallyfollowthatkneeflexionshouldbepositive).CardanSequenceshouldgenerallyfollowfromaxisoflargestexpectedexcursiontoaxisofsmallestexpectedexcursionforthemovementofinterest.Inthebelowexamplewindow,fortherighthipduringwalkingwewouldexpectthelargestexcursiontobeinthesagittal,orX,axis,followedbyfrontal(Yaxis)andtransverse(Z)motion.

Datavariableyou’recreating(musttypeinname)

Whattypeofvariableyou’recreating

Definitionsforvariablecalculation


L.Export_Data_To_Ascii_File:thiscommandisusedtoexportdatatoatab-delimitedtextfilewhichcanbereadinexcelorimportedintoafurtherdataanalysisprogram,suchasMatLab.IntheGUIwindowwhichpopsupwhenyoueditthiscommand,youcandesignatethepathforyourexportedAsciifile,selectoptionssuchasexportingthemeanandstandarddeviationofyourdata(ifyouhavemultipletrials),normalizeyourdata(tomakealltrialsthesamerelativelength),amongotheroptions.Whenyouselectthesignaltype,folder,name,andcomponentyou’dliketoexport,youmustclick“AddaNewSignal”tosavetheseselectionsintothecommand.Likewise,ifyouwishtoeditanexistingcommand,youmustclickthe“ModifySelected”buttonaftermakingyoureditstosavethem.Intheexamplefrom“R_LE_walk_pipeline,”notethateachstrideofdatamusthaveaseparatelydesignatedeventsequenceandthusaseparatelineintheGUI.Ifyourunthiscommand(withanappropriatelyrenamedfilenamepath),youcanopentheexportedfileinexceltoviewtheformatofV3DAsciifiles.If“ExportMeanandStandardDeviation”isselected,themeanandstandarddeviationofalllinesintheGUIwillbecomputed,andthesewillappearascolumns2-7intheAsciiexport.Notethatifyoure-runthe“Export_Data_To_Ascii_File”commandwithoutchangingthe“ExportedAsciiFile”name,youwilloverwriteanyexistingfileatthatfileaddress.V3Dwillnotgivea“areyousureyouwishtooverwrite…”warningdialogbeforeoverwritingexistingdata.


IV.Reports

V3Dcancreatereportstoassistwithdatavisualization.Thesereportscanplotvariablesthathavebeencalculatedandcangenerallybeusefulforcheckingcalculations.Areportisincludedinthe“Reports”tabofthe“R_LE_walk.cmo”fileandthecorrespondingreporttemplatefileisincludedindemonstrationfilesas“R_LE_report_template.rgt.”Thisreportconsistsentirelyof2Dgraphsofjointkinematics.Reportsarealsoabletogeneratetables,bargraphs,andothergraphics.Belowaresomeguidelinesforcreatingareport.

1.Whencreatinganewreportfromscratch,firstdesignatethelocationoftheitem(graph/graphic)youwishtoadd,selectthetypeofitemyouwishtoadd,andclickthe“Add”button.

Locationofitemtoadd

Itemtoadd


2.An“Add/ModifyGraph”windowwillpopup.Hereyoucandesignatewhatdatatographinwhichposition.Belowisanexampleofthiswindowpopulatedforplottingonestrideofsagittalhipangledata.

3.Inareportwithmultiplemovementcyclesandmultiplesvariablesofinterest,therewillbemanylinesinthereportgenerationdialog.Alongwithmultiplecolumnsandrowsofplotsonasinglepage,reportscancontainmultiplepages,allowingforeasyvisualizationoflotsofdata.Ifyouareinterestedingeneratingreports,itisrecommendedthatyouexplorethedocumentationonreportsintheV3Dwiki(https://www.c-motion.com/v3dwiki/index.php/Tutorial:_Creating_a_Report)forfurtherinformationandexamples.




HumanTestingCenter

HumanMotionCore

Chapter2:DelsysTrignoEMG

1. GeneralInformationaboutTrignoSensors1.1. Sensors1-8haveEMG+TriaxialAccelerometersensors.

1.1.1. WhenusedinconjunctionwithQTMthesensorshavecollectionfrequenciesofEMG1925.25Hz(upsampledto2000Hz)and148.48Hz(upsampledto??).

1.2. Sensors9-16haveEMG+TriaxialAccelerometer,GyroscopesandMagnetometers.1.2.1. WhenusedinconjunctionwithQTMthesensorshavecollectionfrequenciesofEMG1925.25Hz

(upsampledto2000Hz)and148.48Hz(upsampledto??).

2. ConfiguringtheTrignoSensors

2.1. Openthe‘DelsysTrignoContorlUtility’2.2. Inthelowerrightcornerofthescreenpress‘Configure’

Addtherestoftheconfigurationscreens–whattheyarehowtheywork.


3. PairingSensors.

3.1. Pairthesensorsyouwouldliketouse3.1.1. Pushthepairbuttonin“DelsysTrignoControlUtility’software.

3.1.2. Pushthebuttononthesensoruntilyouseeaflashinggreenlight.Youshouldalsoreceivea

confirmationonthecomputerscreenthatthesensorhassuccessfullypaired

4. Placementandorientationofsensors5. EnablingSensorsinQTM

5.1. InputDevice

6. LabelingSensorsinQTM


7. AccessingSensordatainQTM

7.1. Sensordatacanbeviewedinrealtime

Updated:16October2017 LivingScienceCore 1

MANUALOFPROCEDURES

LivingScienceCore



Amherst,MA01003

atthe





HumanTestingCenter

LivingScienceCore

Chapter1:OxyconMobile

1.1 Introduction:TheOxyconmobileisaportableindirectcalorimetryunitthatmeasuresmetabolicresponsestoexercise,workorotheractivitiesthatstationarymetabolicunitscannotmeasure.Thewirelesscapabilitiesofthisunitoperateviatelemetry,upto1,000minlineofsightrange.TheOxyconMobileaffordsuserstheopportunityforon-the-spotrecordingofcardiopulmonaryanddeducedparameterssuchas:VO2,VCO2,RER,VEO2,VECO2,PETO2.VE,VT,BF,BR,andSpO2,etc.Researcherscanchoosetomonitorparametersineither,breath-by-breathorintrabreathepochs.Additionally,researcherscanmonitorthesedatainreal-time(viatelemetry),orposthoc,asdataarestoredtoaninternalSDmemorycard.

CoreCompetencies:

1. Beabletofollowcalibrationinstructions2. Understandhowtooutfitparticipantwithequipotent3. Reviewstepsforsettingup,collecting,andreviewingsessiondatathroughascreenreport4. Familiarizeyourselfwithhowtogeneratea.CSVreport

1.2 HowtoCalibrate:Belowisastep-by-stepoutlineforcalibratingtheunit.

PreliminaryCalibrationSteps1.) RetrieveComputerfromOxyconcase

a. Username:labsup Password:lab2.) Collectreceiverunit(picturedbelow)


3.) PluginpowerandconnectcomputertoreceiverunitusinggreyUSBcable


4.) Grabthetwopacks(SBX,DEX)andamemorycarda. SBX:Sensorbox(O2&CO2sensor)b. DEX:Dataexchangeunit

Powercord


5.) WarmupSBXbyconnectingittothereceiverwithyellowEthernetcable

6.) Openlabmanager

a. Allowwarm-upfor15minutes


7.) Assemblenecessarycomponentsofmaska. Attachturbinetoturbineholder(metal(silver)sideinfirst)

b. Makesureturbineisnotwetc. Connectinfraredsensortoturbine

d. Besuresamplingtubeisattachedtotheturbineholder(oppositeendofinfrared)


8.) AttachmaskcomponentsandtightensamplingtubeandinfraredsensortoSBX


9.) PlaceappropriatestudymemorycardinDEXunitandaddbattery(makesureitischarged)

a. IfDEXdoesn’tturnoffrightaway,pressoffbutton

10.) After15minutes,labmanagershouldopenuptoahomescreen


11.) ConnectSBXtoDEXusingyellowEthernetcable

a. AfterafewsecondsofSBXvibrating,alightshouldflashonSBXb. Ifthebatteryislow,SBXwillbeepandanotewillpopuponthescreenc. BesuregreenLED’sonbothunitsarelitpriortocalibration

1.3OxyconMobileCalibration

1. ClickAmbientconditionsonthelabmanager


a. DEXsensesi. Usuallyaccuratetemperatureandbarometricpressureii. Manuallyenterhumidity

b. CheckgreyboxinthelaboratoryontheParvometaboliccartforenvironmentalconditions

i. PressF1tochangetemperature,barometricpressureandhumidityii. Leavebarometricpressureasis

c. PressF12tosave

2. ConnectSBXbacktoreceiverusingyellowEthernetcablea. WaitforSBXtogetpower(willvibrateandLEDlightsupgreenwhenreadytocontinue)

3. SetupforAutoVolumeCalibration

a. ClickAutovolumecalibrationsfromcalibrationsgroup


b. Waitforgraphtocomeup

4. Connectwhitesamplingtube,intoreceiverandSBX


5. ConnectmouthpiecewithturbineintoPCA(blackhole)withsampletubeholecovered

6. PressF1tobeginAutoVolumeCalibration


a. If%differenceislessthan3%pressF12tosaveb. If%differenceisgreaterthan3%,checklinesandconnectionsandretry

7. GasAnalyzerCalibration

a. Connectgascylinderintounit,pushclipuntilitislocked


b. Opengascylinderbytwistingthevalvefromthecalibrationgastwocompleteturns

i. Cylindercontains15.98%O2and3.9%CO2concentrations

c. ClickonGasAnalyzerCalibrationinthelabmanager


d. Clickonthesettingstabatthetopandenterinanalysisresultsfromthe“CertificateofAnalysis”providedbyAirgas,thissheetshouldbewiththegastanks

e. Then,pressF1tobegin,Graphwillappearandcalibrationwillbeginf. Calibrationwillsampleroomairalongwithcylinderair

g. O2peaksat21andvalley;bluelineh. CO2willvalleyat0andthenpeak;redlinei. Ifpercentdifferencelessthan3%pressF12andsavej. Turnofaircylinderk. Ifpercenterrorgreaterthan3%,repeatprocess

8. ReassembleSBXandDEXwithyellowEthernet


9. Reassemblemouthpiecewithsamplingtubea. PlacenewbatteryinDEX


1.4TestingwiththeOxyconMobile

1. Inputpatientdataa. PressF2tocreatenewsubjectb. BesuretofilloutLastName,FirstName,Identification,DOB,Gender,HeightandWeight

(pressentertonavigatethroughpatientinputs)

c. PressF12tosavecurrentsubjectinformation

2. Sizesubjectforheartratebelta. Wetsensorsb. Attachsensorstobeltc. Subjectputsonheartratemonitorensuringthesensorisatthesternum

3. Sizesubjectformask

a. Havesubjectputmaskonandcoverbreathingholeb. Havethesubjectblowtomakesuretherearenoleaksc. Attachthefourclipstothemaskd. Havethesubjectholdthemaskinplacewhiletheresearcherclipstheblackheadgear

nettothemask(taggoesinfront)

4. Sizesubjectforbackpacka. AttachtheSBXandDEXtothebackpack(wiresfacingup)b. Subjectputsonbackpackc. Attachmaskwithwhitesamplingtube(whitesamplingtubeshouldbeclosesttothe

subjectsface)


5. Tobegindatacollection,clickbreathbybreath

a. MakesureDEXandSBXhavegreenlights

i. Ifyellow,flipSBXandDEX,checkHRbeltposition,checkbattery

6. EnsureallPatientDataiscorrect

7. CheckTestsettings


a. Ergometer-Modalitymustbebicycleb. DeadSpace-clickappropriatesizecorrespondingtosubject’smasksizec. Averaging-Method,Breaths

8. PressF1orplaytoanalyzebaselineinformation(backgroundzeroing,notedinbottomrightcorner)

9. PressF1orplayagaintostartrecordingdata10. Afterdatacollectioniscompletebesuretheupperleftbutton(play)isnowa“stop”buttonto

endrecording11. Press12toexitandsave

1.5ReviewingtheData

1. ClickonScreenReporttoviewcollecteddata

2. Fromthereportlist,choosethereportviewyouwouldlike(studyspecific,formoreinformationon

creatingnewdatavisualizationtemplatesseebelow)


3. PressOKtogeneratethereport

4. ToexportusabledatapressF9andsaveonalocaldrive

5. ToimportfromstoragedeviceselectFlashdiskreader


6. Importthefileyouwouldliketoreview7. MakesuretheCurrentpatientmatchesthefileyouaretryingtoimport8. PressF1toreadfile9. AfterfileisloadedpressF12tosave10. RepeatSteps1-411. ToviewsavedfileinExcel,openExcelandgotoFile-Open12. Findthesavedfile13. WhenpromptedbytheImportWizard,MakesureImportRowisat1andFixedWidthisselected

14. ClickNext


15. Whenpromptedwithtextimport,alsoclicknext

16. Onthefinalstep,Clickadvancedandmakesureseparatorsarecorrect

17. Afterimportiscomplete,selectSaveasandbesuretocheckthecorrectfileextension


1.6CleaningEquipment1. Disassembleallpiecestobecleaned2. Wipeturbineholderandinfraredsensorwithalcoholwipes.3. Headgearstrapsandheartratemonitorsshouldberinsedandhunguptodry4. Masksshouldbesoakedina25:1cleaningsolutionfor30secondsandrinsedthoroughly5. Theturbinemustbesoakedinaspecialsolution

a. Inthemarkedcontainer,fillthetothebrimwithSterilizingandDisinfectingsolutionb. Intheclearplasticcuplocatednearby,fillthecuptothefirstindent(blackline)withthe

Activatorsolutionc. PourtheActivatorsolutioninthesterilizingsolutionandmixthoroughlyd. Placetheplasticgrateintothesolutionwiththeturbineinsidee. Allowtheturbinetositfor15minutesf. Removefromsolutionandrinsewithwaterg. Letairdry

1.7CreatingaNewDataVisualizationTemplate1. Clickontheutilitiestab2. Enterpassword:abcd3. Clickonreportdesigner4. Namenewreport,outputunit:defaultprinter(JLABpdfprinter)5. Clickok6. Clickonthetableattheright(hasF3onbutton)

Comment[MB3]:Isthisactuallythepassword?


7. ChooseOxycontable,thenclicksummary8. Hereyoucanchoosemeasurementprogram(breath-by-breath,endtidal,cardiacoutput,intrabreath,

etc.)9. Dependingonthemeasurementprogramselectedyoucanaddparameterstoyoursummary,dothis

byhighlightingaparameteryouareinterestedinandclick“Insert”.Thiswilladdittoyourscreenreportsummary

10. Byfurthernavigatingthroughthetabsatthetopofthedialoguebox,youcanselectaveragingtype(intervals),layout,marker,andsetupsettings

11. PressF12tosavethereport,andnamethereport.Note:thenameyougivethereportwillbehowit

ispresentedinthescreenreportsection(seeReviewingtheDatatore-familiarizeyourselfwiththisstep)


1.8Contacts

1. KimatCarefusion:18002312466Operatorwillpromptyouthroughascreener,click1atfirstquestion,click2atsecondquestion,click3atthirdquestion.

Note:Havereceiverunitonhandwhenyoucall,ithasserialnumbertheywillneedwhenassistingyou.

2. MichaelBusa–CoreDirector413-577-0574

[email protected]




HumanTestingCenter

LivingScienceCore

Chapter2:ActigraphLink(GT9X)

2.1Supplies

1) ActiGraphGTXaccelerometerchargingcable2) ActiGraphdock3) ActiLife6software4) ComputertorunActiLife6software

2.2DeviceCharging

TheActiGraphGT9XaccelerometercontainsarechargeableLithiumPolymerbatterywhichiscapableofprovidingpowerforcontinuousdatacollectionperiodsof10ormoredayswithoutrecharging.Ifthebatteryhasbeenfullydepletedatthetimeofcharging,itwilltakeapproximately3-4hoursfortheActiGraphGT9Xaccelerometertoreachfullcharge.ChargingProcedures

1) ConnectActiGraphdocktocomputerorwalloutletusingminiUSBcable2) PlugtheActiGraphLinkintothedockwiththeActiGraphlogofacingup.Once

connected,theredLEDlightontherightsideofthedockwillturnyellow,thedevicescreenwilldisplaytheserialnumber,andthebattericonwillblinktoindicatecharging.

3) Oncethedeviceisfullycharged,theyellowlightwillturngreenandthebatteryicononthedevicewillshowasfullandstayonsteady

2.3DeviceInitializationTheActiGraphGT9XaccelerometersareinitializedwiththeActiLife6softwarepackage.TheActiGraphGT9XaccelerometerwillnotcollectdataunlessitisinitializedusingtheActiLifesoftware.


InitializationProcedures

1) OpentheActiLife6softwarebydouble-clickingonthedesktopiconorbysingle-clickingonthestartmenushortcutfortheprogram.

2) PlugthechargingcableintoastandardUSBportonthecomputerrunningtheActiLife6

software.3) Selecttheaccelerometerthatwillbeinitializedbycheckingtheboxbesidethe

accelerometerdevicenameandserialnumber.

4) Fromthe“Initialize”buttonchoose“RegularInitialization”.Thiswillopentheprimary

initializationwindow.

1)

3)


5) Selectasamplingrate6) Selectidlesleepmode.7) Note:SelectWirelessOption,heartrate,sleepmode,andIMU8) Clickthe“EnterSubjectInfo”button.Thiswillopenthesecondaryinitializationwindow.

6)

7)

8)

4)

5)


9) Selectastartdateandtimeatthetimesubjectorientationisscheduledtobegin10) Selectastopdateandtimetostopdatacollectionataspecifiedtime(canbeleftblank)

11) Inputanappropriateidentifier(e.g.,“S#”)inthe“SubjectName”field.12) Inputtheappropriate“Limb”and“Side”information(e.g.righthiporleftwrist)13) Clickthe“Initialize1Device”button.Thiswillclosethewindowandreturntothemain

softwarewindow.14) Ifproperlyinitialized,thewords“finishedinitializing”shouldappearunderneaththe

“Status”column.

15) TheActiGraphGT9Xaccelerometerisnowreadyfordatacollection.Unplugaccelerometerfromthechargingcable.

9)

11)

12)

13)


2.4AccelerometerPlacement

TheActiGraphGT9Xcanbewornatthehip(aroundthewaist)andonthewrist.Elasticbeltsandwriststrapswillbeusedtosecuretheaccelerometerstotheparticipant’sbody(seepicturesfordetails).

Securedaroundthewaistattherighthipandattachedtoanelasticbelt.MakesurethattheGT9Xispositioneddirectlyonthebody’sside(mid-axillaryline)

2.5DataRetrievalandDownload

ThepreviouslydescribedActiLife6softwareutilityisrequiredtodownloaddatafromtheActiGraphGT9Xaccelerometer.


DownloadProcedures1) OpentheActiLife6softwarebydouble-clickingonthedesktopiconorbysingle-clickingonthestart

menushortcutfortheprogram.

2) PlugtheActiGraphLinkintothedockwiththeActiGraphgoldplatescontactingthegoldpinsofthedock.3) PlugthechargingcableintoastandardUSBportonthecomputerrunningtheActiLife6software.4) Selecttheaccelerometerthatwillbeinitializedbycheckingtheboxbesidetheaccelerometerdevice

nameandserialnumber.

5) Clickonthe“Download”button.Thiswillopentheprimarydownloadwindow.Click“ChangeLocation”toselectdestinationfile

6) Withinthe“GT9XDownloadOptions”box,makesurethe“CreateAGDFile”boxischeckedandspecifythe“Epoch”.

7) Checktheappropriateboxesforyourprotocol.

1)

4)

5)


8) Clickthe“DownloadAllDevices”button.Theprimarydownloadwindowwillcloseandreturntothemain

softwarewindowanddialogboxwillappearaskingyoutonamethefile.Thedownloadprocessmaytakeseveralminutes.

5)

5))

8)

7)

6)

9)


9) Ifproperlydatawasproperlydownloaded,thewords“finisheddownloading”shouldappearunderneath

the“Status”column.

10) Nowitisnecessarytoprocessthedefaultfilter.agdfile.Select“Scoring”>Checkallitem>click“RemoveSelected”>Click“AddDataset”>SelectthepreviouslydownloadedAGDFile

11) Click“Calculate”12) Goto“Details”onthecalculatedfileandclick“ExportAllEpochs”13) Goto“OpenContainingFolder”,renamethefile(S#_ActiGraph_Location.csv)andputitinthe

appropriatefolder

10)


MANUALOFPROCEDURES

RoomCalorimeterCore



Amherst,MA01003

atthe





HumanTestingCenter

RoomCalorimeterCore

Chapter1:QuickGuideforCalibration/Validation

Supplies

QuickGuide:CalorimeterRoomOperation

GasTankCheckListPriortocalibrationtestsandvalidationstudies,checktankpressures.

Tank MinimumPressure Tests

CO2 100psi Infusion,ShortCircuit,BlenderCalibration

O2 800psiforblendercal,NAforinfusion

Blendercalibration

StartCalRQ Login

SelectMode

HumanorValidationStudy Calibration

CalCheck

Complet

NullTest

No

Yes

RunStudy NullTest

Figure 1. Study Setup Flow


N2 1500forblendercal,800psiforInfusion,500forShortCircuit

Infusion,ShortCircuit,BlenderCalibration

ZeroTank 400psi TankCalibration,CalCheck

SpanTank 400psi TankCalibration,CalCheck

RefTank 400psi Alltests–alwaysopen

Panelinstrumentsettingsforalltests StdSetting

1 Chamber1O2Rotameter 1.25L/min

2 Chamber1CO2Rotameter 1.25L/min

3 Chamber2O2Rotameter 1.25L/min

4 Chamber2CO2Rotameter 1.25L/min

5 InflowO2Rotameter 1.25L/min

6 InflowCO2Rotameter 1.25L/min

7 Chamber1SamplePressureGauge 2psi

8 Chamber1SamplePressureRegulator 2psi

9 Chamber2SamplePressureGauge 2psi

10 Chamber2SamplePressureRegulator 2psi

11 InflowSamplePressureGauge 2psi

12 InflowSamplePressureRegulator 2psi


AnalyzerCalibration

CalCheck:flowzeroandspangasestocheckvalues

AnalyzerGain:Subtracttheapparentconcentrationonthecomputerofthespanvaluefromtheapparentconcentrationonthecomputerofthezerovalue.Thisdeltashouldmatchthedeltaofthespanfromtheblenderortanks(Span(20.95)–Zero(20.05)=0.9delta).

AnalyzerCalibration:Flowzeroandspangases,changeslopeandoffsetvaluesintheanalyzer.Ifanalyzervaluesareoutofrange(<19.8%or>21.2%forO2;>1.1%forCO2)performcalibrationonanalyzers,whenanalyzersarestable,performcalibrationonsoftware,ifneeded.

SoftwareCalibration:Flowzeroandspangases,changeslopeandoffsetvaluesinthesoftware

Delta:Thedifferencebetweenthespanvalueandthezerovalue.Subtracttheapparentconcentrationofthespanvaluedisplayedonthecomputerfromtheapparentconcentrationonthecomputerofthezerovalue.

𝐷𝑒𝑙𝑡𝑎 = 𝑧𝑒𝑟𝑜 − 𝑠𝑝𝑎𝑛 eq(1)0.9 = 20.95 − 20.05 eq(2)

Datafileisrecordingwhentheloggingbutton(J)displays“LoggingOn”andEnterNotebox(K)appears.Filerecordingwillstopwhenloggingbuttondisplays“LoggingOff”or“Done”(L)ispressed.“Done”alsoclosesthewindow.

A

B

C

ED

C

ED

F

IHG

KJ

LFigure2.CalibrationScreen


BlenderCalibration:

1. OpenN2,CO2andO2tanks2. FlipInstrumentationPanelvalvestomatchthoseshowninFigure3.Flipblendervalvesfor

calibration.3. Checkflowrates(1.25lpm)andpressures(~2psi)foreachanalyzer4. Oncethegasisflowing,watchtheO2(A)andCO2(B)graphsuntilthevaluesaresteady.Turn

Averagingon,andletblenderrunforatleastanhour.5. Afterhouriscomplete,pressthe“SetZero”button(D).6. Afterthevaluesarestored,enterthecalibrationtank’sgasconcentrationsintheirrespective

entryboxesinthe“CalGas”column(C)(i.e.ifacalibrationgasconsistingofablendof21.02%O2and0%CO2isflowingintotheanalyzers,21.02wouldbeenteredinthe“CalGas”columnfortheO2analyzersand0wouldbeenteredfortheCO2analyzers).DefaulttankvaluescanbechangedonthePreferencesscreen.

7. Repeatsteps3-6usingtheSpanBlend.8. Selectradiobuttons(F)foreachanalyzeryouwouldliketocalibrate.Buttonswillturnblueif

calculationswillbeperformedforthatanalyzer.9. Afterzeroandspanvalueshavebeenstored,press“Calculate”(G).Newslopesandoffsets

arecalculated,butnotappliedtoanalyzergasvalues.10. Ifslopesandoffsetsareacceptable,press“Apply”(H).Newslopesandoffsetsaresavedto

theconfigurationfile.11. Recordthenewslopeandoffsetvaluesinlabnotebook.12. FlowZerogasblendthroughtheanalyzerstoconfirmthecalibrationvalues.Repeatwiththe

Spangasblend.Ifeitherblenddoesnotmatchthesetvalues,repeatthecalibration.

Figure3.PanelLayoutforBlenderCalibration

Comment[MB4]:Whatareacceptablevalues?


GasTankCalibration

1. Openzeroandspantanks2. FlipvalvesasthoseshowninFigure43. Checkflowrates(1.25lpm)andpressures(~2psi)foreachanalyzer4. Oncethegasisflowing,watchtheO2(A)andCO2(B)graphsuntilthevaluesaresteady.Once

steady,pressthe“SetZero”button(D).5. Afterthevaluesarestored,enterthecalibrationtank’sgasconcentrationsintheirrespective

entryboxesinthe“CalGas”column(C)(i.e.ifacalibrationgasconsistingofablendof21.02%O2and0%CO2isflowingintotheanalyzers,21.02wouldbeenteredinthe“CalGas”columnfortheO2analyzersand0wouldbeenteredfortheCO2analyzers).DefaulttankvaluescanbechangedonthePreferencesscreen.

6. Repeatsteps3-6usingtheSpanCalibrationGas.7. Selectradiobuttons(F)foreachanalyzeryouwouldliketocalibrate.Buttonswillturnblueif

calculationswillbeperformedforthatanalyzer.8. Afterzeroandspanvalueshavebeenstored,press“Calculate”(G).Newslopesandoffsets

arecalculated,butnotappliedtoanalyzergasvalues.9. Ifslopesandoffsetsareacceptable,press“Apply”(H).Newslopesandoffsetsaresavedto

theconfigurationfile.10. Recordthenewslopeandoffsetvaluesinlabnotebook.11. FlowZerogasblendthroughtheanalyzerstoconfirmthecalibrationvalues.Repeatwiththe

Spangasblend.Ifeitherblenddoesnotmatchthesetvalues,repeatthecalibration.

Figure4.PanellayoutforSpanGasCalibration(flipthehighlightedCalibrationSourcevalvefromSpanGastoZeroGasforspancalibration)


TankCalCheck

Purpose:Checkanalyzercalibrationvaluesbeforeastudy(humanandvalidation).

1. OpenZeroandSpancalibrationtanks2. SetpanelupasshowninFigure4.3. Flowzerogastotheanalyzersbyturningthe“CalibrationSource”valveto“ZeroGas”.4. Checkflowrates(1.25lpm)andpressures(~2psi)foreachanalyzer5. Oncethegasisflowing,watchtheO2(A)andCO2(B)graphsuntilthevaluesaresteady.Once

steady,recordthezerovalue.6. Repeatsteps3-4usingthespangas.7. Calculatethegasdeltabysubtractingthezerogasmeasuredontheanalyzerfromthespan

valuemeasuredonthevalue.8.

𝐷𝑒𝑙𝑡𝑎 = 𝑍𝑒𝑟𝑜𝑇𝑎𝑛𝑘𝐺𝑎𝑠𝑃𝑒𝑟𝑐𝑒𝑛𝑡𝑎𝑔𝑒 − 𝑆𝑝𝑎𝑛𝑇𝑎𝑛𝑘𝐺𝑎𝑠𝑃𝑒𝑟𝑐𝑒𝑛𝑡𝑎𝑔𝑒 ex.O2:0.9 = 20.95 − 20.05ex.CO2:0.99 = 0 − 0.99

9. Verifythedeltaiswithin+/-3%oftheexpecteddelta.10. Ifdeltaisnotwithin+/-3%oftheexpecteddelta,performaTanksoftwarecalibration.

Table1.DeltaExample

ExpectedDelta

AllowableDifference

0.9 0.0271 0.03


NullTest

Anulltestverifiestheoffsetofthegasanalyzers.Thetestisperformedbyflowingtheinflowair(medair)toboththeinflowandoutflowanalyzers.AnoffsetcorrectionismadeontheCalibrateAnalyzersscreeninCalRQTMorcanbemadeduringpostprocessing.

1. Openafiletologdata(HumanStudy,ValidationStudy,orCalibration)2. TurntheSampleSourceValvetoNull(Figure5).3. Letthesystemrununtilthehumidityreading,plusVO2andVCO2onthesoftwarestabilize.This

indicatesthatthedryersareconditioned.4. Oncethereadingsarestable,recordthenulldataforatleast10min.5. Ifapplyingthenullcorrectioninrealtime,calculateoffsetcorrectionbysubtractingInflowO2

concentrationfromOutflowO2concentration.RepeatforCO2.ApplyoffsetcorrectiontotheinflowanalyzeroffsetforbothO2andCO2.

Figure5.Panellayoutfornulltest


ValidationTestingShortCircuit

Figure6.ShortCircuit(SC)RackPanelConfiguration.Redboxeshighlightthevalvesthatmustbeturnedforthecorrectgasstreamtoreachtheanalyzers.**ShadedredboxhighlightsthevalvethatdetermineswheretheSCinfusionissent.Turnthevalvetoselectthedesiredchamber**


Figure7.ShortCircuitMFCPanelConfiguration

1. SetuprackpanelasshowninFigure4.ChangeMFCpanelasshowninFigure7

2. Figure9.3. StartaValidationStudy(File->NewValidationStudy)4. ChooseInfusionRadioButton5. EnterValidationID–FilenamewillbeValidationID–Chamber#-Infusion-Date6. SetdesiredInflowandOutflowrates(Edit->ManualMFCSet)

a. InflowRate=60lpmb. OutflowRate=0lpmc. UsethehighflowMFCforchamber2.Setto60lpm

7. EnterflowrateinInflowSettingpress“ApplyInflow”.CheckCurrentInflowRateisasexpected.


8. Runanull–samegasshouldbegoingtoboththeinflowandoutflowanalyzers(Valvesonthepanelshouldbepointingtonull)

9. Checkflowrates(1.25lpm)andpressures(~2psi)foreachanalyzer10. Setchambervolumeto0onthePreferencesScreen.11. OpenBlenderScreen(Edit->AdjustBlender).ChooseFlowRateradiobutton12. SelectAppropriateMFCandsetflowrateorselectdesiredprofile.Press“Apply”tostartflow

throughblender.13. FloweachN2/CO2blendforatleast10minafterVO2andVCO2havestabilized

a. Quickcheck:VCO2shouldbeclosetoCO2flowrate;VO2shouldbecloseto

(N2flow+CO2flow)*[O2inflow]


Infusion

Figure8.InfusionPanelConfiguration-Redboxeshighlightthevalvesthatmustbeturnedforthecorrectgasstreamtoreachtheanalyzers.**Shadedredboxhighlightsthevalvethatdetermineswheretheinfusionissent.Turnthevalvetoselectthedesiredchamber**


Figure9.ChamberStudyMFCPanelSetup

1. SetupRackpanelasshowninFigure6.Selectthecorrectchambertosendgasinfusion.ChangeMFCpanelasshownin

2. Figure93. StartaValidationStudy(File->NewValidationStudy)4. ChooseInfusionRadioButton5. EnterValidationID–FilenamewillbeValidationID–Chamber#-Infusion-Date6. SetdesiredInflowandOutflowrates(Edit->ManualMFCSet)

a. InflowRate=60lpmb. OutflowRate=0lpmc. UsethehighflowMFCforchamber2.Setto60lpm

7. EnterflowrateinInflowSettingpress“ApplyInflow”.CheckCurrentInflowRateisasexpected.


8. Runanull(~15mins)–samegasshouldbegoingtoboththeinflowandoutflowanalyzers(Valvesonthepanelshouldbepointingtonull)

9. Checkflowrates(1.25lpm)andpressures(~2psi)foreachanalyzer10. SetChamberVolumetoappropriatevolume(Chambers=32500liters,Flex=5000liters)11. Closechamberdoor.12. Addanotetodatafilewithchambersetup(whatfurnitureistheroom,platform,bikeor

emptyinthesmallroom)13. SwitchnullvalvetoStudy.Letrunforabout15minutes14. OpenBlenderScreen(Edit->AdjustBlender).ChooseFlowRateradiobutton15. SelectAppropriateMFCandsetflowrateorselectdesiredprofile.Press“Apply”tostartflow

throughblender.16. Setflowratesandselect“Apply”17. VerifyOutflowO2andOutflowCO2valuesarechangingasexpected.