Investigating the Viral-host protein interactome of VP24

VP24

Matrix proteinPlays a role in evading the host immuneresponseLikely to interact with host proteins

Transfected in HEK293T cells

Digested the samples with trypsin

Analyzed peptides by LTQ Orbitrap Velos hybrid ion trap-Orbitrap mass spectrometer

Immunoprecipitated lysates from the cells using GFP-Trap agarose

2 EGFP-labeled VP24 fusion constructs

candidate proteins50

ATP1A1

Inhibitor of ATP1A1, ouabain, reduced viral replication

Sources

Images from shutterstock.com Made with Canva and Adobe Photoshop

Click here to read the blog: "Interrogating the HostInteractome for Ebola Virus Weakness"

Garcia-Dorival, I., et al. (2014, August) “Elucidation ofthe Ebola virus VP24 cellular interactome and disruptionof virus biology through targeted inhibition of host cellprotein function,” Journal of Proteome Research, doi:10.1021/pr500556d [e-pub ahead of print].