Introduction to

Microscopy

Parts of the microscope Eyepiece (9)- the

lens you look through, magnifies your specimen – 10x

Body tube (1)-supports the eyepiece

Arm (10)-supports body tube

Nose piece (2)- holds objective lenses

Objectives (3, 4, 5)- magnify by different powers

Low power objective=4X Medium power

objective=10X High power objective=40X

Stage Clips (6)- holds the slide in place

Aperature-allows light to show through(draw it on the diagram)

Stage (11)- supports the slide being used

Diaphragm (7)- controls the amount of light passing through the opening of the stage (light adjustment wheel)

Course Adjustment knob (12)- moves body tube up and down for focusing, low power

Light source (8)

Fine Adjustment knob (13)- moves body tube up and down for finer focusing, high power

On/Off Switch(draw it on the diagram)

Base (14)- supports the microscope

COMPOUND LIGHT MIRCROSCOPE

Microscopes use lenses to make small objects look larger.

Microscopes must combine magnification and resolution. Magnification - the ability to

make objects look larger than they are

Resolution -the ability to clearly distinguish the individual parts of an object

Safety and Proper Use ALWAYS carry microscope

with two hands – one on the arm and one under the base

Report any problems with microscope first thing

Never touch lenses with finger/hand

Remove slides before returning microscope

NEVER use the Coarse adjustment knob on high power

Total MagnificationTotal Magnification

What is What is total magnification?total magnification? The sum power of all the lenses you are

viewing the specimen with. How do you calculate total

magnification? Eyepiece x objective lens = total mag.

Ex. Our microscopes using medium power. 10 x 10 = 100x (objects appear 100 times

larger than they are.)

Using the Microscope When using the scopes to view slides

it is important to remember to keep the specimen centered!

Keep in mind your field of view Field of View-the area visible through

the microscope lens. Field of view DECREASES as magnification INCREASES.

Adjust the diaphragm as you increase magnification, field of view gets darker.

Using the Microscope Because the light rays from an

object cross before reaching your eye, the image you see through our light microscopes will be inverted and upside down as well as larger and more detailed..

Letter “b” as seen on the stageLetter b as seen through the eyepiece

Image inversion

Because of this image inversion it is important to remember

-When you move your slide right it will move left in the field of vision

-When moving the slide up it will move down in the field of vision

Dissection Scope

A dissection microscope is light illuminated. The image that appears is three dimensional. It is used for dissection to get a better look at the larger specimen. You cannot see individual cells because it has a low magnification.