7/27/2019 Introduction to Surgical Pathology

1/29

7/27/2019 Introduction to Surgical Pathology

2/29

1853 Velpeau , Paris

Ewing, Stout, Willis, AFIP-Fascicles , USA

1950 Lauren Ackerman ,

USA

7/27/2019 Introduction to Surgical Pathology

3/29

7/27/2019 Introduction to Surgical Pathology

4/29

Excisional Biopsy

Incisional Biopsy

Core Needle Biopsy

Breast, lung, soft tissue

tumor

7/27/2019 Introduction to Surgical Pathology

5/29

7/27/2019 Introduction to Surgical Pathology

6/29

FINE Needle Biopsy (FNAB)

A. Non-image guided:

Thyroid gland, breast, salivary gland,

lymph nodes, soft tissue tumor

B.Image guided:

Lungs, paraaortic tumor, retroperitonealmasses, liver

7/27/2019 Introduction to Surgical Pathology

7/29

Thyroid - Benign

7/27/2019 Introduction to Surgical Pathology

8/29

Lung adenocarcinoma

7/27/2019 Introduction to Surgical Pathology

9/29

Paps smear benign

7/27/2019 Introduction to Surgical Pathology

10/29

Paps smear squamous cell carcinoma

7/27/2019 Introduction to Surgical Pathology

11/29

Endoscopic Biopsy

Esophagus, stomach, colon

Stereotactic Biopsy

Brain

7/27/2019 Introduction to Surgical Pathology

12/29

98.58% accuracy

Intra-operative decision-

making

7/27/2019 Introduction to Surgical Pathology

13/29

Gynecological

Paps Smear

Non-gynecological

Other body fluids

Cell Block

7/27/2019 Introduction to Surgical Pathology

14/29

PAS Glycogen(Gastric signet ring adenocarcinoma)

7/27/2019 Introduction to Surgical Pathology

15/29

Mucicarmine Mucin

Reticulin Stain Reticulin Fiber

Bone marrow - AML

7/27/2019 Introduction to Surgical Pathology

16/29

Silver Stain Helicobacter Pylori, Pneumocystis

Carinii

7/27/2019 Introduction to Surgical Pathology

17/29

demonstration of

molecules present on

the surface of specific

cells that allow their

identification

7/27/2019 Introduction to Surgical Pathology

18/29

Substances produced by

tumor cells and released

to the blood

7/27/2019 Introduction to Surgical Pathology

19/29

Cytokeratin epithelial

tumors

CD45 lymphocytic tumorsCD3 T-cell tumors

CD20 B-cell tumorsCD30 Reed Stenberg cell

7/27/2019 Introduction to Surgical Pathology

20/29

Vimentin fibrosarcoma

Desmin Leiomyosarcoma

Actin RhabdomyosracomaChromogranin and

Synaptophysin neuroendocrine

tumor eg. Small cell carcinomaof the lung, medulloblastoma

7/27/2019 Introduction to Surgical Pathology

21/29

Chromogranin

(neuroendocrine tumor)

-only letter B picture

7/27/2019 Introduction to Surgical Pathology

22/29

Glial Fibrillary Acidic Protein

glial cells eg. Astrocytoma

CD117 gastrointestinal stromaltumor

Cadherin lobular carcinoma of

the breast

7/27/2019 Introduction to Surgical Pathology

23/29

ERA, PRA, Her-2-Neu breast Ca

Her-2-Neu gastric Ca

7/27/2019 Introduction to Surgical Pathology

24/29

CEA adenocarcinoma of thecolon

AFP liver

CA 19-9 - tumor of the pancreasand bile duct

PSA prostate cancer

CA-125 mucinouscystadenocarcinoma of theovary

7/27/2019 Introduction to Surgical Pathology

25/29

7/27/2019 Introduction to Surgical Pathology

26/29

7/27/2019 Introduction to Surgical Pathology

27/29

Diploid versus aneuploid

versus polyploid cells are

identified to predict therapidity of the tumor

growth

7/27/2019 Introduction to Surgical Pathology

28/29

Uses cameras attached to

microscope and hooked tothe internet

7/27/2019 Introduction to Surgical Pathology

29/29