Introduction to the Microscope

Care, Parts, FocusingWet-mount SlidesBiological Drawings

Always carry with 2 hands Only use lens paper for cleaning Do not force knobs Always store covered Keep objects clear of desk and cords

A

B

J

D

E

F

G

H

L

M M

LI

C

K

Eyepiece-A

B-Body Tube

C-Revolving NosepieceArm

D- Low Objective LensE- High Objective Lens

Stage-FH-Stage Clips

Coarse Focus-L

Fine Focus- M

Base-K

G-Diaphragm

I, J - Light

Place the Slide on the Microscope

Use Stage Clips Click Nosepiece to the

lowest (shortest) setting Look into the Eyepiece Use the Coarse Focus

MAGNIFICATION

EYE PIECE = 10x

LOW POWER = 10x

HIGH POWER = 40x

TOTAL MAGNIFICATION:

•Low Power: 10x (eye piece) x 10x (low power objective) = 100x

•HIGH Power: 10x (eye piece) x 40x (high power objective) = 400x

Follow steps to focus using low power Click the nosepiece to the longest

objective Do NOT use the Coarse Focusing

Knob Use the Fine Focus Knob to bring the

slide

What can you find on your slide?

Wet-Mount Slides

Materials Needed Compound microscope Slide Cover Slip Dropper Water Specimen

HOW TO MAKE A WET MOUNT SLIDE

1. Collect all materials 2. Place sample on slide 3. Place one drop of water onto the

specimen 4. Place the cover-slip next to the

specimen, holding it at a 45° angle, then drop it slowly down atop the water and specimen. **This is to prevent air bubbles!

Air Bubble

Using your wet-mount slide

Plug the microscope and turn on the lamp.

Before placing the slide under the microscope be sure to Turn the coarse adjustment so the stage

is in its lowest position Turn the revolving nosepiece so the

lowest power objective is in place

On low power, place the slide under the microscope and bring the specimen into focus by turning the coarse adjustment knob.

While looking through the eyepiece, move the slide to the left, move the slide to the right, move the slide away from you.

Bring the specimen back to the center of the field of view and draw what you see.

Switch the microscope to medium power. Use the fine adjustment know to bring into focus. Use the diaphragm to adjust the amount of light that passes through the specimen.

Draw what you see on Medium power.

Switch to highest power objective, and using the FINE ADJUSTMENT KNOW ONLY, bring specimen into focus.

Draw what you see.

Drawing What You See

Now that you’ve viewed your specimen you need to draw it accurately.

The drawing is called a Biological drawing.

BIOLOGICAL DRAWINGS

THE GOOD THE BAD

BIOLOGICAL DRAWING RULES

Use unlined paper. Draw in pencil. Always print. Print the title in capital letters above the field

of view. Drawings and individual structures should

be drawn to scale. When labeling structures on the drawing,

do not cross over lines to point to structures.

BIOLOGICAL DRAWING RULES

Try not to erase. Write the name of the specimen below the field

of view. When using the scientific name of an organism in places other than the title, the first part of the name is capitalized and the second part of the name is not. Underline the scientific name.

Below the name of the organism and to the right, print your name and the total magnification.

BIOLOGICAL DRAWING- Now you try!

PENNYOne cent(4X objective +10X eyepiece)

MICROSCOPE USE REVIEW

1. CARRY CORRECTLY2. STAGE ALL THE WAY DOWN3. START WITH LOW POWER

OBJECTIVE4. USE COARSE FOCUS5. MOVE TO HIGHER POWER6. ONLY USE FINE FOCUS OR YOU

CAN BREAK THE LENS