introduction to the microscope history care parts focusing
TRANSCRIPT
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Introduction to the Microscope
HistoryCarePartsFocusing
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The First Light Microscopes
• Around 1590 Zaccharias and Hans Janssen experimented with lenses in a tube, leading to the forerunner of the microscope and the telescope
• In the late 1600’s, Anton van Leeuwenhoek was the first to see bacteria, yeast, and many other microbes using a microscope
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Images Produced by Light Microscopes
Amoeba Streptococcus bacteria Anthrax bacteria
Human cheek cells Plant cells Yeast cells
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Beyond Light Microscopes
• Light microscopes are limited by their resolution.– Light microscopes cannot
produce clear images of objects smaller than 0.2 micrometers
• The electron microscope was invented in the 1930’s by Max Knott and Ernst Ruska– Electron microscopes use beams
of electrons, rather than light, to produce images
– Electron microscopes can view objects as small as the diameter of an atom
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Types of Electron Microscopes
• Transmission electron microscopes (TEMs) pass a beam of electron through a thin specimen
• Scanning electron microscopes (SEMs) scan a beam of electrons over the surface of a specimen
• Specimens from electron microscopy must be preserved and dehydrated, so living cells cannot be viewed
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Images Produced by Electron Microscopes
Cyanobacteria (TEM) Lactobacillus
(SEM)Campylobacter
(SEM)Deinococcus
(SEM)
House ant Avian influenza virus
Human eyelash Yeast
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• Always carry with 2 hands
• Only use lens paper for cleaning
• Do not force knobs
• Always store covered
• Keep objects clear of desk and cords
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Eyepiece
Body Tube
Revolving NosepieceArm
Objective Lens
StageStage Clips
Coarse Focus
Fine Focus
Base
Diaphragm
Light
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• Place the Slide on the Microscope
• Use Stage Clips • Click Nosepiece to the lowest
(shortest) setting• Look into the Eyepiece• Use the Coarse Focus
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• Follow steps to focus using low power
• Click the nosepiece to the longest objective
• Do NOT use the Coarse Focusing Knob
• Use the Fine Focus Knob to bring the slide
What can you find on your slide?
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References
• http://education.denniskunkel.com/catalog/product_info.php?products_id=1123
• http://micro.magnet.fsu.edu/
• http://inventors.about.com/library/inventors/blroberthooke.htm