introduction to waste water & drinking water microbiology
TRANSCRIPT
©2013 Waters Corporation 1
Introduction to
Waste Water & Drinking Water
Microbiology
©2013 Waters Corporation 2
Moderator: Rebecca Roemer
Market Manager
ERA – A Waters Company
Presenter: Mike Blades
Product Line Manager
Organics and Microbiology
ERA – A Waters Company
©2013 Waters Corporation 3
About ERA
©2013 Waters Corporation 4
Founded in 1977
Golden, Colorado
More than 7,000 laboratories
More than 80 countries
About ERA
©2013 Waters Corporation 5
About ERA
©2013 Waters Corporation 6
About ERA
©2013 Waters Corporation 7
Definitions
Proficiency Testing
Certified Reference Material
©2013 Waters Corporation 8
Mike Blades
Product Line Manager
Organics and Microbiology
ERA – A Waters Company
©2013 Waters Corporation 9
Agenda
Overview of Indicator Organisms
Overview of Regulations
NELAC PT’s
ERA Product Design
Evaluation of Data
Questions
©2013 Waters Corporation 10
Indicator Organisms
The need to determine the suitability of water for drinking & recreational purposes has been long recognized
Simple and reliable methods for the detection & enumeration of microorganisms is necessary
Pathogens themselves are difficult to detect
©2013 Waters Corporation 11
Indicator Organisms
Instead methods have been developed to look for indicator organisms that are frequently associated with these pathogens
Methods are designed to be easier to perform and can be
completed in short periods of time with little expertise in
microbiology
The basic premise: Indicators will be absent from pristine water,
but present if water has been contaminated with waste
Many commonly used Indicators are defined in operational terms
(media and incubation temperature) and are taxonomically
meaningless
©2013 Waters Corporation 12
Indicator Organism Coliforms/Fecal Streptococci
— Total Coliforms
— Fecal Coliforms
— Escherichia coli (E.coli)
— Fecal Streptococci/Enterococci
Regulations have been written specifically for detection of
these indicator organisms
©2013 Waters Corporation 13
Overview of Regulations
The Clean Water Act (CWA) is the cornerstone of surface water
quality protection in the United States
The statute employs a variety of regulatory & non-regulatory tools
to sharply reduce direct pollutant discharges into waterways,
finance municipal wastewater treatment facilities, & manage
polluted runoff
National Pollutant Discharge Elimination System (NPDES)
— Established under Section 402 of the CWA
— As of 2006, Coliforms are now part of the DMR-QA program
nationally
©2013 Waters Corporation 14
Ambient Water Guidelines
— EPA approved test procedures for the following bacteria and
protozoa in ambient waters: Escherichia coli, Enterococci,
Cryptosporidium, & Giardia
BEACH Act
— Amends CWA to reduce the risk of
disease to users of the nation’s
recreational waters
Overview of Regulations
©2013 Waters Corporation 15
Overview of Regulations
Total Coliform Rule (TCR)
— All Public Water Systems
Surface Water Treatment Rule (SWTR)
— Systems using surface water or ground Water
— Heterotrophic Plate Count
Long Term 2 Enhanced Surface Water
Treatment Rule (LT2ESWTR)
— Higher risk systems
http://www.hylabs.co.il http://idexx.com
©2013 Waters Corporation 16
Ground Water Rule
— Establishes multiple barriers to protect against bacteria and
viruses in drinking water from ground water sources
— E. coli/Enterococci
www.millipore.com
Overview of Regulations
©2013 Waters Corporation 17
NELAC PTs
Currently there are only 5 samples defined by NELAC
— Defining information is located on FoPT tables
They are:
— Drinking Water Total/Fecal/E.Coli as Presence/Absence
— Drinking Water Heterotrophic Plate Count
— Drinking Water Total/Fecal/E.Coli as Quantitative
— Non-Potable Water Total/Fecal/E.Coli as Quantitative
— Non-Potable Water Enterococci as Quantitative
©2013 Waters Corporation 18
ERA Product Design
All PT Providers must provide a sample that temporarily stops
bacteria from “normal functions” until you are ready to do the test
Bacteria need a few conditions for normal function. Moisture,
nutrients, appropriate pH and temperature
The lyophilization process removes moisture from the cells, which
means:
— Stable over a large range of temperatures
— Can be shipped anywhere
— Convenience with no time constraints
— Easy hydration step, dissolves easily
CRM Samples are available over any time frame, and are available
after the study closes
CRM samples are derived from our PT samples, actually have
assigned values and acceptance intervals
©2013 Waters Corporation 19
Evaluation of Data
Depends on the type of analyses
— Qualitative vs. Quantitative
Qualitative
— Presence/Absence
Quantitative
— Enumeration of colony forming units
— NELAC evaluation criteria:
o WS HPC (mean ± 2 SD)
o WS Source Water (mean ± 2 SD)
o WP Microbes (mean ± 3 SD)
o WP Enterococci (mean ± 3 SD)
©2013 Waters Corporation 20
Contact ERA
800.372.0122
303.431.8454
6:00 am – 6:00 pm Mon-Thu (MT)
6:00 am – 5:00 pm Fri (MT)
www.eraqc.com
www.facebook.com/eraqc
www.youtube.com/eraqc