ipil ipil leaf meal as supplements to soybean and fish meal

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    Digital re-print - March | April 2009Feature: Ipil ipil

    Feature title: Functional aquaeeds: Ipil ipil lea meal as supplements to soybean and fsh meal

    International Aquafeed is published five times a year by Perendale Publishers Ltd of the United Kingdom.All data is published in good faith, based on information received, and while every care is taken to prevent inaccuracies,

    the publishers accept no liability for any errors or omissions or for the consequences of action taken on the basis ofinformation published.Copyright 2009 Perendale Publishers L td. All rights reserved. No par t of this publication may be reproduced in any formor by any means without prior permission of the copyright owner. Printed by Perendale Publishers Ltd. ISSN: 1464-0058

    THE INTERNATIONAL MAGAZINE FOR THE AQUACULTURE FEED INDUSTRYNEXT PAGE

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    Among plant protein ingre-

    dients, ipil ipil (Leucaena

    leucocephala) leafmeal

    (ILLM) is considered

    the most nutritive plant protein

    source after soybean meal in aquatic

    feeds. That was proven in a 21-day

    experiment conducted to assess the

    response of juvenile Monosex Nile

    tilapia Oreochromis niloticus with four

    iso-nitrogenous formulated di ets: One

    control diet was formulated based on

    fishmeal, one on soybean meal and

    one on rice bran, ipil ipil leafmeal was

    also included in experimental diets.

    Use of 15 percent ILLM in fish feed led

    to a significant improvement from the view

    of growth performance and cost benefit. The

    results indicated that weight and length gain,

    absolute growth, specific growth rate, feed

    conversion efficiency, protein efficiency ratio

    and average daily growth rates were recorded

    significantly highest in the fourth diet (which

    contained 15 percent IILM) compared to

    the other three. The lowest feed conversion

    ratio and cost-per-unit production were also

    recorded to be lowest in the fourth diet.

    On the other hand, the higher cost-

    per-unit fish production was recorded fordiet-1, where ipil ipil leaf meal was absent.

    This study also showed that profit was

    maximised using diet-4, reducing feed costs

    by 24.04 percent.

    The findings in the study demonstrate

    that ipil ipil leafmeal could be used as pro-

    tein substitute at up to 25 percent - opti-

    mumally at 15 percent - in diet of growing

    juvenile monosex tilapia.

    Tilapias are endemic to

    Africa, but interest in their

    aquaculture potential has led

    to their almost worldwide

    distribution over the past 50

    years. Initial enthusiasm was

    based on characteristics that

    made tilapia appropriate for

    subsistence fish farming in

    developing countries: several

    species are herbivorous, readily

    reproduce in small ponds and

    are highly tolerant of poor water quality.

    Interest in commercial production of tila-

    pia was initially dampened by a small harvest

    size resulting from excessive reproduction

    and stunting (Thomas and Leonard, 1995).

    In order to reduce the cost of a balanced

    diet, locally available ingredients, such as

    agricultural by-products and plant proteins,

    should be included in the diet or substituted

    for expensive animal plant protein s ources.

    Legumes such as Ipil ipil are potentially a

    valuable feed resource for aquaculture in the

    tropics because of their widespread distribu-tion in those areas. Most varieties have not

    been evaluated as feed for fish. Leucaena leu-

    cocephala compares favourably with lucerne

    in terms of crude protein (CP), calcium (Ca)

    and phosphorus (P), and it is also a good

    source of b-carotene. (NAP 1984).

    By production volume, tilapia culture is

    the second largest aquaculture system in

    use worldwide in sweet-water and is mostly

    carried out using semi-intensive systems

    in developing countries (FAO, 2000). Nile

    Tilapia (Oreochromis niloticus) was introduced

    first in Bangladesh in 1954 from Thailand

    (Ahmed, 1956).

    In populations of tilapia, males grow faster

    and are more uniform in size than females.

    For this reason, the farming of monosex

    populations of tilapias, which is achieved

    either by manual sexing, direct hormonal sex

    reversal, hybridisation or genetic manipula-

    tion, has been reported as solutions to theproblem of early sexual maturation and

    unwanted reproduction.

    As in most fish culture systems, balanced

    feed is one the most significant inputs in tilapia

    culture and accounts for

    between 30-60 percent of

    production costs (El-Sayed,

    1990; Goddard, 1996; Tudor

    et al 1996). Substitution of

    fishmeal in balanced feed has

    been vital to reducing feed

    costs and overall tilapia pro-

    duction costs. Efforts have

    been made to reduce this

    by Dr Hossain Zamal, Prabal Barua, Belal Uddin

    Table 1: Ingredient composition of experimental diet

    Ingredients Diet-1(%)Diet-2

    (%)Diet-3

    (%)Diet-4

    (%)

    Fish meal 33 30 30 35

    Soybean meal 35 33 30 25

    Rice bran 32 17 15 25

    Ipil ipil leaf meal(leucaena leaf)

    ---- 20 25 15

    Estimation of growth and financial analysis through the application of Ipil ipil (Leucaena leucocephala) leaf

    meal as supplements to soybean and fish meal in the diet of juvenile monosex tilapia (Oreochromis niloticus)

    Ipil ipil leaf meal as supplementsto soybean and fish meal

    Figure 1: Ipil Ipil leafmeal preparation process

    36 | InternatIonalAquAFeed | Mch-api 09 - i

    Ipil ipil

    input cost by substituting

    fishmeal with a wide vari-

    ety of vegetables and meats

    (Olvera-Novoa et al 1990;

    Olvera-Novoa et al 1997;

    El-Saidy and Gaber, 2003).

    The larvae of monosex

    tilapia are habitat to feed

    at surface. So, experimental

    diets were prepared ingranulated form for the purpose of achieving

    better floating characteristics.

    Methodology:

    A: Experimental locationThe experiment was performed for a

    period of 21 days at a nursery pond of

    Allahwalla Hatchery and Fisheries Project,

    Coxs Bazar, Bangladesh.

    B: Collection & preparationIpil ipil leaf (IIL) collection and prepara-

    tion: The ipil ipil tree was cultivated in the

    dyke and roadside of the research station.

    The green, soft and fleshy leaves were col-

    lected from the tree and Figure-1 shows theprocedure of Ipil ipil leafmeal preparation.

    C: Diet preparationC.1: Ingredient selection: Four ingredi-

    ents - fishmeal, soybean meal (SBM), Ipil ipil

    Leafmeal and rice bran - were selected for

    experimental diet preparation. The fishmeal

    and soybean meal were used as protein

    source. The ipil ipil leafmeal was partially

    incorporated with fishmeal and soybean as

    protein sources. Rice bran was selected as

    a carbohydrate and energy source. All the

    ingredients were sieved to remove larger

    particles. The fishmeal and soybean meal

    were imported from Thailand by Allahwala

    Hatchery and Fisheries Project for nursery

    feed as reliable protein sources. Rice bran was

    collected from a local market.

    C.2: Diet Formulation: Four isonitrog-

    enous diets, which contained a crude protein

    value 30 percent, were formulated as shown

    in Table 1. The protein requirement was

    balanced using the square method (Pearson,

    1976). The blended feeds were used as

    experimental diets and stored in an airtight

    polythene bag for longer term use. Four

    different ingredients: fishmeal, soybean meal

    (SBM), Ipil-Ipil Leafmeal and rice bran were

    selected for experimental diet preparation

    based on their composition. The control diet

    (Diet-1) was formulated without mixing of

    Ipi ipil leafmeal. Table-2 shows the proximate

    composition of the four ingredients.

    D: Experimental designThe experiment was conducted in four

    'hapa' each 3 1.5 0.45 m (L W

    H). Each 'hapa' contained three similar

    chambers for replication in order to study

    the growth of monosex tilapia fry with

    different experimental diets. Diet-1 (was

    denoted as control); Diet-2, Diet-3 and

    Diet-4 were respectively applied in 'hapa-1',

    'hapa-2', 'hapa-3' and 'hapa-4' for comparative

    study of their effect on growth.

    E: Larvae collection & stockingThe juvenile monosex tilapias were col-

    lected from nursery pond of the research

    station with an average weight of 0.634g

    and length of 3.52cm. A numerous fry

    were stocked in cemented tank without

    Table 2: Proximate composition of feed ingredients

    IngredientsCrudeProtein

    (%)

    CrudeFat(%)

    CrudeFibre(%)

    Ash(%)

    Moisture(%)

    NitrogenFree

    Extract(NFE)

    Ipil-Ipil leaf meal - (IILM) 23 8 16 10 14 29

    Fish meal 55 6 8 8 15 8Soybean meal 38 7 13 9 12 21

    Rice bran 11 10 30 11 13 25

    Ipil- Ipil

    Ipil-ipil, (Leucaena leucocephala) is a small Mimosoid tropical tree native to Mexico, one

    of the most versatile species of all tropical trees because of its exceptionally high rate

    of growth, ability to fix nitrogen and high nutritional value as feed for livestock (Hughes,

    Colin E., 1998 ). It has been conside red for biomass production , as its reported yield of foliage

    corresponds to a dried mass of 2000-20000 kg/ha/year, and that of wood 30-40 m/ha/year,

    with up to twice those amounts in favourable climates.

    High rates of nitrogen fixation (100-500 kg N/ha/yr), which contribute to high foliar N

    content (4.3% dry wt.), and rapid decomposition of its foliage make ipil-ipil an excellent mulch

    (surface application) or green manure (incorporated) crop. In addition to inorganic nutrients,

    ipil-ipil mulch/green manure can increase soil organic matter (Duke 1983). During the 1970s

    and 1980s it was promoted as a "miracle tree" for its multiple uses It has also been described

    as a "conflict tree" in that it is both promoted for forage production and spreads like a weed in

    some places ( Gutteridge and Shelton, 1998).

    Classification of Ipil- Ipil

    Kingdom : Plantae Division : Magnoliophyta Class : Magnoliopsida Order : Fabales Family : Fabaceae Subfamily: Mimosoideae Genus: Leucaena Species: L. leucocephala,

    (Lam.). de Wit

    - i Mch-api 09 | InternatIonalAquAFeed | 37

    Ipil ipil

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    (percent) was recorded for Diet-3. The

    higher fat 7.63 (percent) was recorded

    for Died-1 and lower fat 7.4 (percent)

    was recorded for Diet-3. The huger

    Ash 9.24 (percent) was recorded for

    Diet-1 and lower Ash 9.24 (percent) was

    recorded for Diet-2. The higher NFE

    19.7 (percent) was recorded for Diet-

    2 and lower NFE 18.0 (percent) was

    recorded for Diet-1 (see Table-1).

    Table-3 represents the growth and

    nutrient utilisation of juvenile

    monosex tilapia on the test

    diets. There were significant dif-

    ferences (P 0.05).

    Highest MWG, MLG, SGR, AG, ADG, FCE

    and PER were found in fish fed Diet-4. The

    least MWG, MLG, SGR, AG, ADG wereobserved in fish fed Diet-1, while the least

    I: Bio-Chemical EvaluationMoisture, ash, lipid and protein content

    of the ingredients were determined by

    standard AOAC methods (AOAC, 1995).

    J: Feed EconomicsThe economical feasibility of the experi-

    mental diets was studied by analysed unit

    cost and total costs

    of all ingredientswere used in the

    experiments.

    Results

    The nutrient ele-

    ments, such as crude

    protein, crude fat,

    crude fibre, ash con-

    tents, moisture con-

    tents and NFE were

    evaluated for each of

    the isonitrogenous

    diets for the suitabil-

    ity of their finished

    product for mono-

    sex tilapia fry. The

    four experimentaldiets were contained 35 percent crude

    protein. The higher moisture content

    13.6 (percent) was recorded for Diet-

    4 and lowest moisture content 13.31

    (percent) was recorded for Diet-1. The

    higher crude fibre 16.79 (percent) wasrecorded for Diet-1 and lower value 14.8

    feed for one day. Total 2400 uniform size

    fry were taken into 12 polythene bags.

    The fry within polythene bags were accli-

    matised with water temperature of the

    'hapa' for three-to-five minutes. Just after

    acclimatised the fry were finally stocked

    into 'hapa'. Each 'hapa' contained 600 fry

    and each experimental chamber contained

    200 fry.

    F: Feed managementDue to the feeding behaviour of

    tilapia, granulated experimental diets

    were spread on centre of each chamber

    by a small plate arrangement to avoid

    feeding competition between exterior

    fish. The amount of feed supplied to the

    reared fry was 25 percent of the total

    biomass per day for the first 10 days and

    20 percent of total biomass per day for

    second 11 days.

    G: Periodical observationThe growth and survival of the reared

    specimen in each 'hapa' with three

    replicates were recorded at seven days

    interval. Ten percent of the estimated fry

    were sampled and collected samples were

    placed on the well-marked stillness scale

    and recorded the total length from the

    extreme mouth to the tip of tail. 20 spe-

    cies were taken from each chamber for

    measuring total length. The mean weightof the sample was then determined by

    dividing the total weight by the num-

    bers of animals.

    H: Biological evaluationMean weight gain (MWG), mean

    length gain (MLG), absolute growth

    (AG) and specific growth rate (SGR)

    were determined as advocated by

    Sedgwick (1979). Feed conversion ratio

    (FCR), feed conversion efficiency (FCE),

    protein efficiency ratio (PER) were

    calculated as described by Castell and

    Tiews (1980).

    Table 3: Growth performance of juvenile tilapia in experimentaldiets

    parameter Diet-1 Diet-2 Diet-3 Diet-4

    Abso lu te Growth (g) 919.26 929.2 979.26 1119.26

    Specific Growth Rate(SGR)

    5.86% 5.90% 6.05% 6.52%

    Feed ConversionRatio (FCR)

    2.74 2.73 2.78 2.42

    Feed ConversionEfficiency (%)

    36.43 36.67 35.99 41.23

    Protein EfficiencyRatio

    1.04 1.047 1.028 1.178

    Survival Rate ( %)) 100 100 100 100

    Table 4: Feed formulation cost (1 kg fish) and cost per unit production ( Tk/kg)

    Diets

    Feedformulatingcost (Tk /

    kg)

    Totalfeedused(kg)

    Totalfeedcost(Tk)

    Total fishproduction

    at the end ofexp.(kg)

    Costper unit

    production(Tk/kg)

    Diet-1 20.82 2.523 52.53 1.152 45.6

    Diet-2 18.33 2.534 46.45 1.31 35.46

    Diet-3 17.85 2.721 48.57 1.36 35.71

    Diet-4 19.15 2.714 51.97 1.5 34.65

    38 | InternatIonalAquAFeed | Mch-api 09 - i

    Ipil ipil

    Discussion:

    Ingredients selection is one of the most

    important factors for formulation and com-

    mercial production for supplemental quality

    feed of aquatic species. In the present study

    ingredient were selected to consider their

    nutritional quality and cost effectiveness.

    Ipil leafmeal. The

    higher formulating

    cost was 20.82tk/

    kg for Diet-1 and lower formulating cost

    was 17.85tk/kg for Diet-3. The estimated

    higher cost for production of 100kg juve-

    nile monosex tilapia was four, 560taka*

    for Diet-1 and lower cost for production

    of 100kg juvenile monosex tilapia was

    3465taka for Diet-4. The higher benefit

    was 24.01 percent (taka) was estimated

    for Diet-4 and lower benefit 21.69 per-

    cent (taka) was estimated for Diet-3

    when compared with Diet-1.

    FCR, FCE and PER were observed in Diet-

    4, Diet-3 and Diet-2 respectively.

    In the present experiment, feed prepa-

    ration cost (Tk/kg) and proportional ben -

    efit analysis among different experimental

    diets (Tk/kg) were observed in the Table

    4 and Table 5; The lowest production c ost

    indicating economic viability of the feed.

    The higher price of feed ingredients was

    37.00tk/kg for fishmeal and lower price

    for ingredients was 3.00tk/kg for Ipil-

    Table 5: Comparative Benefit Analysis among differentexperimental diet

    Diet

    Cost per unitproductionof fish ( taka

    / kg)

    TotalBenefit(taka)

    % ofBenefit

    Diet-1 45.6 ------- -----

    Diet-2 35.46 10.14 22.24

    Diet-3 35.71 9.89 21.69

    Diet- 4 34.65 10.95 24.01

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    T HE FA C E of HEALTH

    T f F : :

    * This article refers to Bangladesh Currency (Taka)

    1 Bangladeshi Taka (BDT) = 0.01424 US Dollar (USD)

    1 Bangladeshi Taka (BDT) = 0.009804 British Pound (GBP)

    1 Bangladeshi Taka (BDT) = 0.01109 Euro (EUR)

    (exchange rate correct at time of printing)

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    - i Mch-api 09 | InternatIonalAquAFeed | 39

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    tion cost of experimental diets were found

    highest 20.82tk/kg for Diet-1 and lowest

    17.85tk/kg for Diet-3 and also the amount

    of cost per unit production was highest

    45.6tk/kg for Diet-1 and lowest 34.65tk/

    kg for Diet-3. The lowest production cost

    indicating economic viability of the feed

    (Table 3 and 4). Partial replacement of

    fishmeal or marine animal protein and

    soybean meal by ipil ipil leafmeal resultedin better growth performance, indicating an

    economical profit for juvenile tilapia.

    In commercial aquaculture, feed cost is

    the major part of overall production cost.

    Benefit of aquaculture is calculated with

    per unit production and cost of feed for it.

    The achievement of the present study was

    that 24 percent feed cost were reduced by

    using experimental Diet-4 which contained

    15 percent IILM (non conventional feed

    ingredients) in the diet.

    ConclusionsTilapia culture in rural area of developing

    countries requires strategies for better

    performance.

    From the overall discussion of the

    present experimental results it has estab-

    lished that better growth and minimum feed

    cost of reared species may also be obtained

    using the feed with mixed protein of plant

    and animal origin.

    Supplementation of artificial diets to the

    natural food may further increase the growth

    and survival and decrease the feed conversion

    of the rearing species. To achieve a balance

    nutritional composition in fish feed, a more

    diverse choice should be made in selecting feed

    ingredients. A mixture of feed ingredients will

    provide more balance nutrients than only use

    limited feed ingredients to formulate fish feed.

    Products derived from ipil ipil have been

    shown to be important ingredients for prac-

    tical feeds for tilapia. This is primarily because

    of the high level of protein, its low price andthe case of incorporation into feeds and the

    low level of anti-nutritional elements. The

    findings in the present study shown that ipil

    ipil leafmeal could be used as protein sub-

    stitute up to 25 percent and optimum level

    15 percent in diet of growing tilapia. IILM at

    15 percent level used in the diets have good

    nutritive values and have a significant role on

    the growth, FCR, FCE and all of the perform-

    ance measure of O. niloticus.

    Acknowledgement:The authors are deeply indebted and

    grateful to Master Abul Kashem, proprietor

    be obtained using the Diet-4 mixed with

    protein of plant and animal origin. The result

    of the present study is in closer agreement

    with the report of Djunaidah et al 1986

    found the best result in growth with diets,

    which were composed of fishmeal 35 per-

    cent and soybean meal 30 percent.

    In evaluating Leucaena leucocephala

    leafmeal as a protein source in Indian major

    carp, Labeo rohita, Hassan et al (1994)observed a trend of reduced performance

    with the highest growth, in terms of weight

    in fish fed diets with 25 percent soaked

    leucaena diets.

    Vogt et al (1986) found that growth and

    survival of the tiger prawn was better fed

    diets in which 20 percent of the soybean

    was replaced with Leucaena leucocephala

    leaves. Ghatnkar et al (1983) reported that

    diets with 30-65 percent Leucaena leuco-

    cephala had no adverse affects on growth or

    reproductive behaviour of Mossabique tilapia

    (S. mossambicus) and Indian major carps

    (Labeo rohita, Cirrhinus mrigala and Catla catla)

    although the diets did not improve their

    performance over that of a standard diet.

    When Rahman et al (1988) fed Nile tilapia

    (T. niolotica) a diet containing Leucaena leuco-

    cephala leaves; the fish grew more slowly than

    those on a standard rat diet. The leaves also

    caused alterations in the female gonads. In the

    present study it was found that Ipil ipil leafmeal

    at 15 percent level used in the diets have good

    nutritive values and have a significant role on

    the growth, FCR, FCE and all of the perform-

    ance measure of Oreochormis niloticus.

    It also found that FCR increased with

    increased in ipil ipil leafmeal level in the

    diet, being highest in Oreochormis niloticus

    fed 25 percent ILLM and lowest in tilapia 15

    percent for both optimum and high energy

    diets. Tilapia showed very poor growth

    when Leauceana leucocephala constituted

    25 percent or more of the dietary protein.

    The reduced value of FCR is very significantfor commercially and economically and also

    prove the better growth performance.

    Better performance in growth, nutrient

    utilisation and proximate composition in O.

    niloticus fed diets with leaf protein concen-

    trates showed that leaf proteins made from

    leucaena could be viable means of improving

    fish feed specially for Oreochormis niloticus. The

    proximate compassion of the feed ingredients

    and ipil ipil leafmeal used in the experimental

    diets in the present studies were within the

    expected ranges (Ayuk et al 2002; Nas, 1977;

    Mutayoba et al 2003; Adeparusi et al 2005)

    In the present experiment, feed prepara-

    Jackson et al (1982) reported that the

    success of commercial aquaculture system

    depends on proper selection of feed ingredi-

    ents that should be contain optimum level of

    protein and energy, necessary for the growth

    of the respective culture species and the

    ingredients should also be low cost.

    In the present study, ingredients were

    selected to considered their nutritional

    quality and cost effectiveness.

    Pascual (1980) reported that not only

    the quantity of protein but also the quality

    of protein cause the difference in weight

    gain between the crude ipil ipil leafs and

    soaked ipil ipil leaf. The soaked ipil ipil leaf

    extracted harmful mimosine and achieved

    better weight gain than crude ipil ipil leaf

    which content toxic element mimosine. He

    observed that fishmeal and ipil ipil leavemeal

    was the best attractant followed by fish.

    The present experimental result of abso-

    lute growth (AG) on Oreochromis niloticus

    showed the greater value of AG is more

    significant for commercially economically.

    Better growth of the reared tilapia may also

    40 | InternatIonalAquAFeed | Mch-api 09

    Ipil ipil

    :

    - i Mch-api 09 | InternatIonalAquAFeed | 5

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