lab meeting 2014.02.24 이세라. gene scan –b2m sample name 등록번호 sample 날짜 양회식...
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![Page 1: Lab meeting 2014.02.24 이세라. Gene Scan –B2M Sample name 등록번호 Sample 날짜 양회식 7095224211/09 CRS 환자 신원홍 7095184911/16 CRS 환자 PCR](https://reader035.vdocuments.net/reader035/viewer/2022062321/56649f315503460f94c4d022/html5/thumbnails/1.jpg)
Lab meeting
2014.02.24이세라
![Page 2: Lab meeting 2014.02.24 이세라. Gene Scan –B2M Sample name 등록번호 Sample 날짜 양회식 7095224211/09 CRS 환자 신원홍 7095184911/16 CRS 환자 PCR](https://reader035.vdocuments.net/reader035/viewer/2022062321/56649f315503460f94c4d022/html5/thumbnails/2.jpg)
Gene Scan –B2MSample name 등록번호 Sample 날짜
양회식 70952242 11/09 CRS 환자
신원홍 70951849 11/16 CRS 환자
PCR
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Sample 2.Sample 1.
electrophoresis
95℃ / 5min
![Page 4: Lab meeting 2014.02.24 이세라. Gene Scan –B2M Sample name 등록번호 Sample 날짜 양회식 7095224211/09 CRS 환자 신원홍 7095184911/16 CRS 환자 PCR](https://reader035.vdocuments.net/reader035/viewer/2022062321/56649f315503460f94c4d022/html5/thumbnails/4.jpg)
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Sequencing-PLK1
Childhood AML -RNA
![Page 6: Lab meeting 2014.02.24 이세라. Gene Scan –B2M Sample name 등록번호 Sample 날짜 양회식 7095224211/09 CRS 환자 신원홍 7095184911/16 CRS 환자 PCR](https://reader035.vdocuments.net/reader035/viewer/2022062321/56649f315503460f94c4d022/html5/thumbnails/6.jpg)
cDNA합성
65 ℃ / 5min -> ice
25 ℃ / 10min -> 50 ℃ / 50min->85 ℃ / 5min -> ice
1μl of Rnase H 37 ℃ / 20min -> ice
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cDNA->electrophoresis
cDNA PCR->electrophoresis
![Page 8: Lab meeting 2014.02.24 이세라. Gene Scan –B2M Sample name 등록번호 Sample 날짜 양회식 7095224211/09 CRS 환자 신원홍 7095184911/16 CRS 환자 PCR](https://reader035.vdocuments.net/reader035/viewer/2022062321/56649f315503460f94c4d022/html5/thumbnails/8.jpg)
PLK1 -Primer
![Page 9: Lab meeting 2014.02.24 이세라. Gene Scan –B2M Sample name 등록번호 Sample 날짜 양회식 7095224211/09 CRS 환자 신원홍 7095184911/16 CRS 환자 PCR](https://reader035.vdocuments.net/reader035/viewer/2022062321/56649f315503460f94c4d022/html5/thumbnails/9.jpg)
pyrosequencing
A-365B-2009-129D-4
D-22
2014.02.24(BDNF)
PCR전 PCR후2013.09.24(SLC6A4)
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flowcytomety2014.02.21
G-MDSCM-MDSC
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G-MDSCM-MDSC
2/20sample 조일도 71411924