lc columns inertsil - hichrom · isolated silanol si-oh 'eminalsilanol3i /() 2 silica-gel...

HICHROMChromatography Columns and Supplies

Hichrom Limited1 The Markham Centre, Station RoadTheale, Reading, Berks, RG7 4PE, UK

Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484Email: [email protected] www.hichrom.co.uk

Catalogue 9

LC COLUMNSInertsil

LC Columns - GL Sciences

www.hichrom.co.uk • [email protected] • Tel: 0118 930 3660 107

Hichrom Lim

itedGL Sciences

• Enhanced sensitivity• High resolution• Excellent for acidic, basic and chelating compounds• Compatible with up to 100% aqueous eluents

Phase Endcapped Particle Size (μm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%)

ODS-4 and ODS-4V Yes 2, 3, 5 100 450 11

C8-4 Yes 2, 3, 5 100 450 5

Inertsil 4 Series Phases

The Inertsil® 4 series is based on enhanced deactivation techniques which produce highly inert silica, enabling successful analysis of trace levels of the most demanding basic and acidic compounds. Strictly classified particle size, with narrow distribution width and an ideal carbon loading, enables high efficiency columns to be guaranteed. Figure 3 illustrates the high sensitivity LC-MS/MS separation of haloacetic acids, harmful substances generated when tap water is chlorinated.

Inertsil ODS-4 and C8-4 columns also show excellent stability during the analysis of strong metal chelating compounds. Figure 4 shows the separation of tetracyclines, veterinary antibiotics with very strong chelating characteristics.

The 3μm phases are also available in High Pressure (HP) format. Please enquire for details.

Ordering Information – Inertsil 4 Series

Inertsil PhaseColumn Dimensions1 (mm) Guard Cartridges2 (2/pk)

(10 x 1.5mm)30 x 2.1 50 x 2.1 75 x 2.1 100 x 2.1 150 x 2.1 250 x 2.12μm £424 £424 £468 £511 £584 - -ODS-4 5020-81200 5020-81202 5020-81203 5020-81204 5020-81205 - -C8-4 5020-81280 5020-81282 5020-81283 5020-81284 5020-81285 - -3μm £335 £335 £345 £347 £379 £411 £183ODS-4 5020-04011 5020-04012 5020-04013 5020-04014 5020-04015 5020-04016 5020-19302C8-4 5020-03971 5020-03972 5020-03973 5020-03974 5020-03975 5020-03976 5020-19347

- £379 £392 £396 £434 £472 -ODS-4V - 5020-30212 5020-30213 5020-30214 5020-30215 5020-30216 -5μm £279 £279 £291 £291 £323 £357 £183ODS-4 5020-03911 5020-03912 5020-03913 5020-03914 5020-03915 5020-03916 5020-19301C8-4 5020-04051 5020-04052 5020-04053 5020-04054 5020-04055 5020-04056 5020-19346


(10 x 4.0mm)30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 250 x 4.63μm £335 £335 £345 £347 £379 £411 £161ODS-4 5020-04041 5020-04042 5020-04043 5020-04044 5020-04045 5020-04046 5020-19002C8-4 5020-03992 5020-03993 5020-03994 5020-03995 5020-03996 5020-03997 5020-19047

- £379 £392 £396 £434 £472 -ODS-4V - 5020-30242 5020-30243 5020-30244 5020-30245 5020-30246 -5μm £279 £279 £291 £291 £323 £357 £161ODS-4 5020-03941 5020-03942 5020-03943 5020-03944 5020-03945 5020-03946 5020-19001C8-4 5020-04081 5020-04082 5020-04083 5020-04084 5020-04085 5020-04086 5020-19046

- - - - £379 £418 -ODS-4V - - - - 5020-10901 5020-10902 -

1 Other dimensions available 2 Use with guard cartridge holder 5020-08500 (£97)

Figure 3. Analysis of haloacetic acids by LC-MS/MS

Column: Inertsil ODS-4 (3μm, 150 x 2.1mm)Eluent: A: CH

3OH B: 0.1% HCOOH

Gradient: A/B = 5/95 – 2.5min – 5/95 – 2.5min – 95/5 – 5min – 95/5Flow rate: 0.2ml/minTemperature: 40°CDetection: LC-MS/MS (ESI, negative, MRM)

1. Bromoacetic acid2. Dibromoacetic acid3. Tribromoacetic acid4. Chloroacetic acid5. Dichloroacetic acid6. Trichloroacetic acid7. Bromochloroacetic acid

Figure 4. Analysis of tetracyclines by LC-MS/MS

Column: Inertsil ODS-4 (3μm, 75 x 2.1mm)Eluent: CH

3OH B: 0.1% HCOOH

Gradient: A/B = 20/80 – 4.5min – 20/80 – 2min – 95/5 – 3.5min – 95/5Flow rate: 0.2ml/minTemperature: 40°CDetection: LC-MS/MS (ESI, positive, MRM)

1. Tetracycline (TC)2. Oxytetracycline (OTC)3. Chlortetracycline (CTC)4. Doxycycline (DC)

12

3

4

5

6

71

2

43

0 1 2 3 4 5 6 7 Mins8 9 10 11 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 Mins4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5

Inertsil® 4 Series

Please contact Hichrom for current prices. Tel: +44 (0)118 930 3660 Email: [email protected]

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• High purity base silica (99.999%)• Large surface area• Exceptional stability and column lifetime• Validated ODS-3 column

Compared with Inertsil® 2 Series, Inertsil 3 Series phases are based on a purer, higher surface area silica which is specially manufactured to provide maximum phase coverage. The result is a series of columns which provide excellent peak shapes using simple eluents while operating at low pressure.

Phase Endcapped Particle Size (μm) Pore Size (Å)Surface Area

(m2/g)Carbon Load (%)

Recommended pH Range*

SIL - 3, 5 100 450 - 2 - 7.5

ODS-3 Yes 2, 3, 4, 5, 10 100 450 15 2 - 7.5

ODS-3V Yes 3, 5 100 450 15 2 - 7.5

ODS-P No 3, 5 100 450 29 2 - 7.5

ODS-EP No 5 100 450 9 2 - 7.5

Peptide C18 Yes 5 100 450 15 2 - 7.5

ODS-Sprint Yes 3, 5 100 450 8.5 2 - 7.5

Sulfa C18 Yes 3, 5 100 450 15 2 - 7.5

C8-3 Yes 2, 3, 5 100 450 9 2 - 7.5

Ph-3 No 2, 3, 5 100 450 9.5 2 - 7.5

CN-3 No 3, 5 100 450 14 2 - 7.5

NH2 No 3, 5 100 450 8 2 - 7.5

Diol No 3, 5 100 450 22 2 - 7.5

HILIC No 3, 5 100 450 20 2 - 7.5

Amide No 3, 5 100 450 18 2 - 7.5

AX No 5 100 450 17 2 - 7.5

CX No 5 100 450 14 2 - 7.5

Inertsil 3 Series Phases

*Inertsil phases are known to provide excellent results at pH levels of 9 – 10. However, optimum column life may be achieved at a pH between 2 and 7.5.

Inertsil ODS-3Peak tailing of basic components in reversed-phase is frequently the result of residual silanol groups. GL Sciences developed a proprietary bonding endcapping technology to eliminate these silanols. To ensure maximum inertness, each batch of Inertsil ODS-3 must pass a QC test using 29Si NMR. After ODS bonding, the magnitude of the isolated silanols is greatly decreased. After endcapping, the isolated silanols have been completely eliminated, thereby assuring excellent inertness and optimum performance of ODS-3 (see Figure 5).

Inertsil ODS-3V – a validated Inertsil columnInertsil ODS-3V is a highly efficient, validated HPLC column, developed to provide optimum separation for a wide range of acidic and basic compounds. Each lot of packing undergoes a series of critical QC analytical tests, consistent with the demands of GLP/GMP compliance, to confirm:

1) the absence of surface silanols2) minimal metallic residues3) inertness to basic compounds4) reproducible separations

Figure 5. Measurement of residual silanol groups by 29Si NMR

Figure 6. Separation of antidepressant drugs on Inertsil ODS-3V

Column dimensions: 250 x 4.6mm Flow rate: 1ml/min.Eluent: CH

3OH - 25mM KH

2PO

4, pH 6 (60:40) Temperature: 25°C

1. Norephedrine2. Nortriptyline3. Toluene4. Imipramine5. Amitriptyline

1 2

4

3

5

Isolated silanol Si-OH

Geminal silanol Si-(OH)2

Silica-gel structure Si-(O-Si)4

ODS group C18

H37

-Si-O- Remaining silanol

Endcapped groups (CH

3)3-Si-O-

Bare silica gel support After ODS bonding After ODS bonding and endcapping

Inertsil® 3 Series

0 2 4 6 8 10 12 14 Mins16 18



Hichrom Lim

itedGL Sciences

• Polymerically bonded C18• Improved separation of planar compounds• Useful for carotenoid analysis• Separation of the 16 EPA PAHs

Inertsil® ODS-P is a polymerically bonded phase which is densely bonded with octadecylsilane groups. It shows unique separation capabilities and offers an alternative selectivity to Inertsil ODS-3. Inertsil ODS-P is particularly suited to the separation of polycyclic aromatic hydrocarbons and shows additional retentivity for planar compounds.

Figure 7 shows the separation of terphenyl isomers on three types of ODS phases. On the polymeric Inertsil ODS-P phase, the planar p-terphenyl is retained longer than the non-planar o- and m- isomers. Figure 8 shows the separation of components from toad venom on the ODS-P phase.

Inertsil® ODS-P

• C18 with polar embedded group• Compatible with 100% aqueous eluents• Alternative selectivity to C18• Ideal for LC-MS

Inertsil ODS-EP

Inertsil ODS-EP contains a polar functional group embedded between the silica surface and the C18 group. This creates a stable phase, which is resistant to phase collapse (or pore dewetting) in 100% aqueous eluents. It provides highly reproducible separations for acids and bases in organic eluents as well as acidified eluents typically used in LC-MS. Figure 9 shows a comparison of the selectivity of Inertsil ODS-EP with that of a C18 phase.

Figure 7. Separation of terphenyl isomers

Column dimensions: 150 x 4.6mmEluent: CH

3CN - H

2O (85:15)

Flow rate: 1ml/minTemperature: 40°CDetection: UV, 254nm

Figure 8. Separation of toad venom

Figure 9. Comparison of Inertsil ODS-EP and C18 columns*

Column: Inertsil ODS-P (250 x 4.6mm)Eluent: CH

3CN - 0.1% H

3PO

4 (45:55)

Flow rate: 1ml/minTemperature: 40°C

Column dimensions: 150 x 4.6mmEluent: CH

3CN - H

2O (70:30)

Flow rate: 1ml/minColumn temperature: 40°C

Monomeric (Inertsil ODS-3) Intermediate (Inertsil ODS-2) Polymeric (Inertsil ODS-P)

o-terphenyl (non-planar) m-terphenyl

(non-planar)

o-

m-

p-o-

m-

p-

o-

m-

p-

1. Bufalin

2. Cinobufagin

3. Resibufogenin

4. Indomethacin

21

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Urac

il Ethy

lben

zene

Naph

thal

ene

Prop

ylben

zene

Buty

lben

zene

Anth

race

ne

Urac

il Ethy

lben

zene

Naph

thal

ene

Prop

ylben

zene

Buty

lben

zene

Anth

race

ne

Inertsil® 3 Series (continued)

0 2 4 6 8 10 12 0 2 4 6 8 10 12 0 2 4 6 8 Mins

0 10 20 30 Mins

Inertsil ODS-EP Alkyl-C18

p-terphenyl (planar)

*The comparative separations presented here may not be representative for all applications.

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• Balanced retention of polar and non-polar compounds• Compatible with 100% aqueous eluents• Low back pressure

Inertsil® ODS-Sprint is super base deactivated and optimally bonded to retain polar compounds without excessive retention of non-polar compounds. This phase shows excellent peak shapes for a wide range of polar analytes, including both basic and acidic compounds. In Figure 10 the hydrophobic compounds are eluted in approximately two-thirds the time of Inertsil ODS-3, whilst maintaining retention of polar compounds. Inertsil ODS-Sprint operates at low back pressures, even at high flow rates and shows excellent robustness and reproducibility.

Inertsil® ODS-Sprint

Inertsil Peptide C18The Inertsil Peptide C18 column is specifically suited to the analysis of peptide digests. Figure 11 shows the application of this column to the analysis of cytochrome C tryptic digest.

Inertsil Sulfa C18Inertsil Sulfa C18 (3 and 5μm) is designed for the analysis of sulpha drugs. In addition, it is effective in the screening of synthetic antibacterial agents and residues in products of animal origin, as directed by food safety investigative guidelines. Each batch of Inertsil Sulfa C18 is tested for the effective separation of sulpha drugs, as shown in Figure 12.

Figure 10. Polar and non-polar mixture Figure 11. Analysis of cytochrome C tryptic digest on Inertsil Peptide C18

Figure 12. Analysis of 15 sulfa reagents

1. Uracil2. Caffeine3. Phenol4. n-Butylbenzene5. o-Terphenyl6. n-Amylbenzene7. Triphenylene

Column: Inertsil ODS-Sprint and Inertsil ODS-3Column dimensions: 250 x 4.6mmEluent: CH

3OH - H

2O (80:20)


Column: Inertsil Peptide C18 (100 x 2.1mm)Eluent: A: H

2O - CH

3CN (95:5) + 0.05% TFA

B: CH3CN - H

2O (80:20) + 0.04% TFA

Gradient: 5 - 50% B in 30 minsFlow rate: 0.2ml/minSample: 160pmol cytochrome C tryptic digest

1. Sulfanilamide2. Sulfacetamide3. Sulfadiazine4. Sulfathiazole5. Sulfapyridine6. Sulfameradine7. Sulfacymidine8. Sulfamethoxypyridazine

9. Sulfamonomethoxine10. Sulfachlorpyridazine11. Sulfamethoxazole12. Sulfabenzamide13. Sulfadimethoxine14. Sulfaquinoxaline15. Sulfanitran

Column: Inertsil Sulfa C18 (3μm, 150 x 4.6mm)Eluent: A: CH

3CN – CH

3CO

2H - (777.66:3.15, w/w)

B: CH3CO

2H – H

2O (3.15:997, w/w)

100% B to 67% B in 33mins then to 10% B in 4minsFlow rate: 1ml/minTemperature: 40°CSample: 1ppm each

1

2

43

5

6

7

1

2

43

5 6

7

Inertsil ODS-3

Inertsil ODS-Sprint

1 2

4

3

56 7 8

911

1012

1314

15


0 10 20 30 Mins 0 10 20 30 Mins

0 10 20 30 40 Mins



Hichrom Lim

itedGL Sciences

• Unique selectivity for polar compounds• High sensitivity LC-MS analyses• Low back pressure• Applications for amino acids, water-soluble vitamins and drug metabolites

Inertsil HILIC is a propyl alcohol bonded silica phase, suitable for the analysis of polar compounds in the hydrophilic interaction (HILIC) mode. It has also shown promise in SFC applications. Unlike traditional reversed-phase materials, HILIC columns retain highly polar compounds with only small amounts of water in the eluent. The high organic solvent composition of the eluent will lead to high sensitivity LC-MS analyses, due to a decrease in ion suppression. It also results in low back pressure and the ability to run at high flow rates.

Figure 13 shows the separation of a mixture of oligosaccharides on Inertsil HILIC. Inertsil HILIC has also been used in the development of a fast and sensitive LC-MS/MS method for the analysis of melamine and cyanuric acid contaminants in pet food samples (see Figure 14).

Inertsil® HILIC

• Unique selectivity in both reversed-phase and normal-phase• Applications for amino acids, water-soluble vitamins and sugars

Inertsil Diol

The Inertsil Diol phase is a dihydroxypropyl bonded silica which can be used in both reversed-phase and normal-phase modes. Figure 15 shows the analysis of free amino acids on an Inertsil Diol column.

• Excellent for polar compounds in HILIC mode• Ideal for LC-MS/MS• Long column lifetime

Inertsil Amide

Inertsil Amide is a carbamoyl bonded silica phase designed for the separation of highly polar compounds by HILIC. Inertsil Amide columns offer significantly increased retentivity and a unique selectivity in separating compounds that were unresolvable on other columns.

Column: Inertsil Sulfa C18 (3μm, 150 x 4.6mm)Eluent: A: CH

3CN – CH

3CO

2H - (777.66:3.15, w/w)

B: CH3CO

2H – H

2O (3.15:997, w/w)

100% B to 67% B in 33mins then to 10% B in 4minsFlow rate: 1ml/minTemperature: 40°CSample: 1ppm each

Figure 13. Analysis of oligosaccharides on Inertsil HILIC

1. Maltose2. Maltotriose3. Maltotetraose4. Maltopentaose5. Maltohexaose6. Maltoheptaose

Column: Inertsil HILIC (5μm, 250 x 4.6mm)Eluent: CH

3CN - H

2O (65:35)


Figure 14. Analysis of melamine and cyanuric acid on Inertsil HILIC

Column: Inertsil HILIC (150 x 3.0mm)Eluent: A: 10mM ammonium acetate in CH

3CN

B: 10mM ammonium acetate in H2O

Gradient: T(mins) %A %B 0 97 3 5.0 20 80 5.5 3 97Flow rate: 0.5ml/minTemperature: 40°CDetection: MS/MS (ESI)

Figure 15. Analysis of free amino acids

1. Phenylalanine2. Methionine3. Valine4. Dopa5. Alanine6. Serine7. Citrulline

Column: Inertsil Diol (250 x 4.6mm)Eluent: CH

3CN - H

2O (80:20)

Flow rate: 1ml/minColumn temperature: 40°C

Cyanuric Acid(Negative ESI)

Melamine(Positive ESI)

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6

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2

4

3

5

6

7


0 2 4 6 8 10 0 3.0 3.5 4.0 4.5 5.0 Mins5.5Mins

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• Silica based ion-exchange columns• High reproducibility• High efficiency• Exceptional stability and long column lifetime

Inertsil AX and CX are silica based anion- and cation-exchange phases respectively, based on high purity silica. They show good reproducibility, exceptional stability and long column lifetime. Figure 16 shows the separation of nucleotides on Inertsil AX and Figure 17 shows the analysis of nucleic acid bases by cation-exchange chromatography on Inertsil CX.

Inertsil® Ion-Exchange Phases

Ordering Information – Inertsil 3 Series

Inertsil ODS-3 (Hichrom Manufactured Columns)

Inertsil PhaseColumn Dimensions (mm)

100 x 4.6 150 x 4.6 250 x 4.6

ODS-3 3μm INODS3-3-100D £307 INODS3-3-150D £329 INODS3-3-250D £361

ODS-3 5μm INODS3-100D £252 INODS3-150D £274 INODS3-250D £307

Please contact Hichrom for availability of other 3 Series phases and alternative dimension columns.

Microbore (2.1mm i.d.) Columns (GL Sciences Manufactured)


30 x 2.1 50 x 2.1 75 x 2.1 100 x 2.1 150 x 2.1

2μm £395 £409 £453 £497 £570

C8-3 5020-84930 5020-84931 5020-84932 5020-84933 5020-84934

ODS-3 5020-84650 5020-84652 5020-84653 5020-84654 5020-84655

Ph-3 5020-85130 5020-85131 5020-85132 5020-85133 5020-85134


(For 2.1mm i.d. Columns)50 x 2.1 100 x 2.1 150 x 2.1 250 x 2.1

3μm £285 £307 £329 £361 £183

C8-3 5020-04812 5020-04814 5020-04815 5020-04816 5020-19315

ODS-3 5020-04412 5020-04414 5020-04415 5020-04416 5020-19305

ODS-P 5020-04662 5020-04664 5020-04665 5020-04666 5020-19309

ODS-Sprint 5020-02812 5020-02814 5020-02815 5020-02816 5020-19307

Ph-3 5020-05012 5020-05014 5020-05015 5020-05016 5020-19317

CN-3 5020-05262 5020-05264 5020-05265 5020-05266 5020-19319

NH2 5020-05462 5020-05464 5020-05465 5020-05466 5020-19321

Diol 5020-05412 5020-05414 5020-05415 5020-05416 5020-19323

SIL 5020-04212 5020-04214 5020-04215 5020-04216 5020-19327

HILIC 5020-07762 5020-07764 5020-07765 5020-07766 5020-19325

Amide 5020-07862 5020-07864 5020-07865 5020-07866 5020-201601 Other column dimensions available 2 Use with free standing holder 5020-08500 (£97)

Figure 16. Analysis of nucleotides on Inertsil AX

1. CMP2. AMP3. UMP4. GMP5. XMP

Column: Inertsil AX (150 x 4.6mm)Eluent: 0.03M KH

2PO

4 (pH 5.0 with H

3PO

4)


Figure 17. Analysis of nucleic acid bases on Inertsil CX

1. Uracil2. Thymine3. Guanine4. Cytosine5. Adenine

Column: Inertsil CX (150 x 4.6mm)Eluent: 0.2M ammonium formate, pH 5.0Flow rate: 1ml/minTemperature: 40°C

1

2

4

3

5

1

2

43

5


AX bonded phase structure CX bonded phase structure

— N(CH2CH3)2 — C6H4SO3- H+

0 10 20 30 40Mins 0 10 20Mins




Hichrom Lim

itedGL Sciences

Ordering Information – Inertsil 3 Series (continued)Microbore (2.1mm i.d.) Columns (GL Sciences Manufactured) (continued)



3μm £329 £357 £384 £422 £183ODS-3V 5020-30112 5020-30114 5020-30115 5020-30116 5020-19305

£322 £361 £383 £409 £183Sulfa C18 5020-07502 5020-07504 5020-07505 5020-07506 5020-19313

4μm £257 £279 £301 £333 £183ODS-3 5020-04612 5020-04614 5020-04615 5020-04616 5020-19304

5μm £230 £252 £274 £307 £183C8-3 5020-04912 5020-04914 5020-04915 5020-04916 5020-19314

ODS-3 5020-04512 5020-04514 5020-01741 5020-01742 5020-19303

ODS-P 5020-04712 5020-04714 5020-04715 5020-04716 5020-19308

ODS-EP 5020-02612 5020-02614 5020-02615 5020-02616 5020-19310

ODS-Sprint 5020-02712 5020-02714 5020-02715 5020-02716 5020-19306

Ph-3 5020-05112 5020-05114 5020-05115 5020-05116 5020-19316

CN-3 5020-05312 5020-05314 5020-05315 5020-05316 5020-19318

NH2 5020-05512 5020-05514 5020-05515 5020-05516 5020-19320

Diol 5020-05612 5020-05614 5020-05615 5020-05616 5020-19322

SIL 5020-04312 5020-04314 5020-04315 5020-04316 5020-19326

HILIC 5020-07702 5020-07704 5020-07705 5020-07706 5020-19324

Amide 5020-07802 5020-07804 5020-07805 5020-07806 5020-20159

£278 £307 £329 £361 £183Sulfa C18 5020-07542 5020-07544 5020-07545 5020-07546 5020-19312

£383 £392 £398 £433 £183AX 5020-07212 5020-07214 5020-07215 5020-07216 5020-19333

£420 £438 £450 £504 £183CX 5020-07112 5020-07114 5020-07115 5020-07116 5020-19334

£278 £325 £347 £380 £183Peptide C18 5020-08022 5020-08024 5020-08025 5020-08026 5020-19311

Medium bore (3.0mm i.d.) Columns (GL Sciences Manufactured)


30 x 3.0 50 x 3.0 75 x 3.0 100 x 3.0 150 x 3.02μm £395 £409 £453 £497 £570C8-3 5020-84935 5020-84936 5020-84937 5020-84938 5020-84939

ODS-3 5020-84660 5020-84662 5020-84663 5020-84664 5020-84665

Ph-3 5020-85135 5020-85136 5020-85137 5020-85138 5020-85139


(For 3.0mm i.d. Columns)50 x 3.0 100 x 3.0 150 x 3.0 250 x 3.03μm £285 £307 £329 £361 £161C8-3 5020-04822 5020-04824 5020-04825 5020-04826 5020-19115

ODS-3 5020-04422 5020-04424 5020-04425 5020-04426 5020-19105

ODS-P 5020-04672 5020-04674 5020-04675 5020-04676 5020-19109

ODS-Sprint 5020-02822 5020-02824 5020-02825 5020-02826 5020-19107

Ph-3 5020-05022 5020-05024 5020-05025 5020-05026 5020-19117

CN-3 5020-05272 5020-05274 5020-05275 5020-05276 5020-19119

NH2 5020-05472 5020-05474 5020-05475 5020-05476 5020-19121

Diol 5020-05422 5020-05424 5020-05425 5020-05426 5020-19123

SIL 5020-04222 5020-04224 5020-04225 5020-04226 5020-19127

HILIC 5020-07772 5020-07774 5020-07775 5020-07776 5020-19125

Amide 5020-07872 5020-07874 5020-07875 5020-07876 5020-20152

£329 £357 £384 £422 £161ODS-3V 5020-30122 5020-30124 5020-30125 5020-30126 5020-19105

£322 £361 £383 £409 £161Sulfa C18 5020-07512 5020-07514 5020-07515 5020-07516 5020-19113

4μm £257 £279 £301 £333 £161ODS-3 5020-04622 5020-04624 5020-04625 5020-04626 5020-19104

1 Other column dimensions available 2 Use with free standing holder 5020-08500 (£97)


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Ordering Information – Inertsil 3 Series (continued) Medium bore (3.0mm i.d.) Columns (GL Sciences Manufactured) (continued)


(For 3.0mm i.d. Columns)50 x 3.0 100 x 3.0 150 x 3.0 250 x 3.05μm £230 £252 £274 £307 £161C8-3 5020-04922 5020-04924 5020-04925 5020-04926 5020-19114ODS-3 5020-04522 5020-04524 5020-01751 5020-01752 5020-19103ODS-P 5020-04722 5020-04724 5020-04725 5020-04726 5020-19108ODS-EP 5020-02622 5020-02624 5020-02625 5020-02626 5020-19110ODS-Sprint 5020-02722 5020-02724 5020-02725 5020-02726 5020-19106Ph-3 5020-05122 5020-05124 5020-05125 5020-05126 5020-19116CN-3 5020-05322 5020-05324 5020-05325 5020-05326 5020-19118NH2 5020-05522 5020-05524 5020-05525 5020-05526 5020-19120Diol 5020-05622 5020-05624 5020-05625 5020-05626 5020-19122SIL 5020-04322 5020-04324 5020-04325 5020-04326 5020-19126HILIC 5020-07712 5020-07714 5020-07715 5020-07716 5020-19124Amide 5020-07812 5020-07814 5020-07815 5020-07816 5020-20151

- - £329 £368 £161ODS-3V - - 5020-01821 5020-01822 5020-19103

£278 £307 £329 £361 £161Sulfa C18 5020-07552 5020-07554 5020-07555 5020-07556 5020-19112

£383 £392 £398 £433 £161AX 5020-07222 5020-07224 5020-07225 5020-07226 5020-19133

£420 £438 £450 £504 £161CX 5020-07122 5020-07124 5020-07125 5020-07126 5020-19134

£278 £325 £347 £380 £161Peptide C18 5020-08032 5020-08034 5020-08035 5020-08036 5020-19111

Analytical (4.6mm i.d.) Columns (GL Sciences Manufactured)


(For 4.6mm i.d. Columns)50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.63μm £285 £307 £329 £361 £161C8-3 5020-04842 5020-04844 5020-01911 5020-01912 5020-19015ODS-3 5020-01774 5020-01775 5020-01771 5020-01772 5020-19005ODS-P 5020-04692 5020-04694 5020-04695 5020-04696 5020-19009ODS-Sprint 5020-02842 5020-02844 5020-02845 5020-02846 5020-19007Ph-3 5020-05042 5020-05044 5020-01931 5020-01932 5020-19017CN-3 5020-05292 5020-05294 5020-05295 5020-05296 5020-19019NH2 5020-05492 5020-05494 5020-05495 5020-05496 5020-19021Diol 5020-05442 5020-05444 5020-05445 5020-05446 5020-19023SIL 5020-04242 5020-04244 5020-01701 5020-01702 5020-19027HILIC 5020-07792 5020-07794 5020-07795 5020-07796 5020-19025Amide 5020-07892 5020-07894 5020-07895 5020-07896 5020-20148

£329 £357 £384 £422 £161ODS-3V 5020-30142 5020-30144 5020-30145 5020-30146 5020-19005Sulfa C18 5020-07532 (£322) 5020-07534 (£361) 5020-07535 (£383) 5020-07536 (£409) 5020-190134μm £257 £279 £301 £333 £161ODS-3 5020-04642 5020-04644 5020-04645 5020-04646 5020-190045μm £230 £252 £274 £307 £161C8-3 5020-04942 5020-04944 5020-01900 5020-01901 5020-19014ODS-3 5020-01763 5020-01765 5020-01731 5020-01732 5020-19003ODS-P 5020-04742 5020-04744 5020-02001 5020-02002 5020-19008ODS-EP 5020-02642 5020-02644 5020-02645 5020-02646 5020-19010ODS-Sprint 5020-02742 5020-02744 5020-02745 5020-02746 5020-19006Ph-3 5020-05142 5020-05144 5020-01920 5020-01921 5020-19016CN-3 5020-05342 5020-05344 5020-01940 5020-01941 5020-19018NH2 5020-05542 5020-05544 5020-05545 5020-05546 5020-19020Diol 5020-05642 5020-05644 5020-05645 5020-05646 5020-19022SIL 5020-04342 5020-04344 5020-01711 5020-01712 5020-19026HILIC 5020-07732 5020-07734 5020-07735 5020-07736 5020-19024Amide 5020-07832 5020-07834 5020-07835 5020-07836 5020-20147

£278 £307 £329 £368 £161ODS-3V - - 5020-01801 5020-01802 5020-19003Sulfa C18 5020-07572 5020-07574 5020-07575 5020-07576 (£361) 5020-19012

£383 £392 £398 £433 £161AX 5020-07242 5020-07244 5020-07245 5020-07246 5020-19033

£420 £438 £450 £504 £161CX 5020-07142 5020-07144 5020-07145 5020-07146 5020-19034

£278 £325 £347 £380 £161Peptide C18 5020-08052 5020-08054 5020-08055 5020-08056 5020-19011

1 Other column dimensions available 2 Use with free standing holder 5020-08500 (£97)




Hichrom Lim

itedGL Sciences

• High purity 300Å silica phases• Excellent reproducibility• Good physical and chemical stability

Inertsil® Wide Pore Phases

The Inertsil® WP300 series of columns has been developed for the analysis and purification of proteins and peptides. It currently consists of five phases, which are available in column dimensions ranging from capillary to preparative.

Inertsil WP300-C8 and C18 show high separation efficiency for peptides and proteins of medium molecular size (recommended <5,000 Da for C18 phase and <20,000 Da for C8 phase). Inertsil WP300-C4 can elute hydrophobic proteins >5,000 Da with excellent peak shape due to the shielding effects of embedded polar groups. Figure 18 shows the separation of a polypeptide mixture on Inertsil WP300-C8.

Please contact Hichrom for ordering information for Inertsil wide pore phase columns.

Inertsil Wide Pore PhasesInertsil WP300 Phase

Bonding EndcappedParticle Size

(μm)Pore Size (Å)

Surface Area (m2/g)

Carbon Load (%)

pH Range

SIL - - 5 300 150 - 2 - 7.5

C4 Polar embedded butyl Yes 5 300 150 3 2 - 7.5

C8 Octyl Yes 5 300 150 8 2 - 7.5

C18 Octadecyl Yes 5 300 150 9 2 - 7.5

Diol Propyldiol No 5 300 150 9 2 - 7.5

Figure 18. Analysis of polypeptide mixture

Column: Inertsil WP300-C8 (150 x 4.6 mm)Eluent: A: H

2O (0.1% TFA)

B: CH3CN-H

2O (90:10) (0.1% TFA)

Gradient: A:B 80:20 to 40:60 over 25 minsTemperature: 30°C

1. Ribonuclease2. Insulin3. Cytochrome4. Lysozyme5. BSA6. Trypsin Inhibitor from Soybean7. Ovalbumin

• Excellent efficiency and column-to-column reproducibility• Easy installation• Easy coupling with MS

Capillary and Nano Columns

GL Sciences produce a range of capillary and nano columns for high sensitivity trace analyses. These columns are available for InertSustain, Inertsil 4 Series, Inertsil 3 Series and Inertsil wide pore materials as well as Titansphere products, which are designed specifically for applications involving phosphopeptide extractions (see page 118).

The Capillary EX columns are available in 0.3, 0.5 and 0.7mm i.d. and 50 or 150mm lengths. These columns are packed using glass-lined stainless steel tubing of 1/ 4˝ o.d. and come with stainless steel end fittings.

Capillary EX-Nano columns are available with 0.05, 0.075, 0.1 and 0.2mm i.d. with lengths of 50, 150 and 250mm. These columns are packed using fused silica tubing and inserted into 1/ 16˝ o.d. PEEK tubing for protection, with stainless steel end fittings.

Please contact Hichrom for ordering information for Inertsil Capillary EX and Capillary EX-Nano columns.

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ukGL Sciences

• Spherical porous silica• Excellent for acidic and basic molecules• Well referenced in the scientific literature

The well established Inertsil® 2 Series phases are based on high purity silica which is robust and shows high reproducibility from batch-to-batch and column-to-column.

Inertsil® 2 Series and Inertsil ODS

Inertsil Phase Functional Group Endcapped Particle Size (μm) Pore Size (Å)Surface Area

(m2/g)Carbon Load (%)

ODS-2 Octadecyl Yes 5 150 320 18.5

C8 Octyl Yes 5 150 320 10.5

C4 Butyl Yes 5 150 320 7.5

Phenyl Phenyl Yes 5 150 320 10

ODS* Octadecyl Yes 5 100 350 14

Inertsil 2 Series and Inertsil ODS Phases

* From original Inertsil series

Ordering Information – Hichrom Manufactured ColumnsMicrobore (1.0mm i.d.) Columns

Inertsil PhaseColumn Dimensions1 (mm) Guard Cartridges2


ODS-2 INODS2-50M £375 INODS2-100M £390 INODS2-150M £405 INODS2-250M £435 INODS2-10CE5 £110 (5/pk)3

C4 INC4-50M £435 INC4-100M £455 INC4-150M £465 INC4-250M £495 INC4-10CE5 £110 (5/pk)3

Phenyl INP-50M £385 INP-100M £400 INP-150M £415 INP-250M £445 INP-10CE5 £110 (5/pk)3



ODS-2 INODS2-50AM £350 INODS2-100AM £365 INODS2-150AM £380 INODS2-250AM £410 INODS2-10CM5 £110 (5/pk)3

C4 INC4-50AM £410 INC4-100AM £425 INC4-150AM £440 INC4-250AM £470 INC4-10CM5 £110 (5/pk)3

Phenyl INP-50AM £360 INP-100AM £375 INP-150AM £390 INP-250AM £420 INP-10CM5 £110 (5/pk)3

Microbore (2.1mm i.d.) Columns

Medium Bore (3.2mm i.d.) Columns



ODS-2 INODS2-50AS £325 INODS2-100AS £340 INODS2-150AS £355 INODS2-250AS £385 INODS2-10C5 £110 (5/pk)3

C4 INC4-50AS £385 INC4-100AS £400 INC4-150AS £415 INC4-250AS £445 INC4-10C5 £110 (5/pk)3

Phenyl INP-50AS £335 INP-100AS £350 INP-150AS £365 INP-250AS £395 INP-10C5 £110 (5/pk)3

Analytical (4.0mm i.d.) Columns



ODS-2 INODS2-50AF £325 INODS2-100AF £340 INODS2-150AF £355 INODS2-250AF £385 INODS2-10C5 £110 (5/pk)3

C4 INC4-50AF £385 INC4-100AF £400 INC4-150AF £415 INC4-250AF £445 INC4-10C5 £110 (5/pk)3

Phenyl INP-50AF £335 INP-100AF £350 INP-150AF £365 INP-250AF £395 INP-10C5 £110 (5/pk)3

Analytical (4.6mm i.d.) Columns



ODS-2 INODS2-50A £325 INODS2-100A £340 INODS2-150A £355 INODS2-250A £385 INODS2-10C5 £110 (5/pk)3

C4 INC4-50A £385 INC4-100A £400 INC4-150A £415 INC4-250A £445 INC4-10C5 £110 (5/pk)3

Phenyl INP-50A £335 INP-100A £350 INP-150A £365 INP-250A £395 INP-10C5 £110 (5/pk)3

1 Other column dimensions available – please enquire 2 Use with free-standing holder HI-161 (£45) and column coupler HI-081 (£26) – see p. 24 3 Starter kits (£145) also available – see p. 25

Semi-Preparative and Preparative (7.75 – 21.2mm i.d.) ColumnsPlease contact Hichrom for further details of 7.75 – 21.2mm i.d. Inertsil columns.




Hichrom Lim

itedGL Sciences

Ordering Information – GL Sciences Manufactured Columns (Inertsil 2 Series and Inertsil ODS)Microbore (2.1mm i.d.) Columns

Inertsil 5μm PhaseColumn Dimensions1 (mm) Guard Cartridges2 (2/pk)

(For 2.1mm i.d. Columns)150 x 2.1 250 x 2.1

£347 £383 £183

ODS-2 5020-01121 5020-01125 5020-19335

£392 £420 £183

C8 5020-01221 5020-01225 5020-19336

Phenyl 5020-01321 5020-01325 5020-19337

£438 £475 £183

C4 5020-01421 5020-01425 5020-19338

£528 £573 £183

ODS 5020-02121 5020-02125 5020-19341

Medium Bore (3.0mm i.d.) Columns


(For 3.0mm i.d. Columns)150 x 3.0 250 x 3.0

£347 £383 £161

ODS-2 5020-01122 5020-01126 5020-19135

£392 £420 £161

C8 5020-01222 5020-01226 5020-19136

Phenyl 5020-01322 5020-01326 5020-19137

£438 £475 £161

C4 5020-01422 5020-01426 5020-19138

£528 £573 £161

ODS 5020-02122 5020-02126 5020-19141

Analytical (4.0 and 4.6mm i.d.) Columns


(For 4.0 and 4.6mm i.d. Columns)150 x 4.0 250 x 4.0 150 x 4.6 250 x 4.6

£347 £383 £347 £383 £161

ODS-2 5020-01123 5020-01127 5020-01124 5020-01128 5020-19035

£392 £420 £392 £420 £161

C8 5020-01223 5020-01227 5020-01224 5020-01228 5020-19036

Phenyl 5020-01323 5020-01327 5020-01324 5020-01328 5020-19037

£438 £475 £438 £475 £161

C4 5020-01423 5020-01427 5020-01424 5020-01428 5020-19038

£528 £573 £528 £573 £161

ODS 5020-02123 5020-02127 5020-02124 5020-02128 5020-190411 Other column dimensions available 2 Use with free-standing holder 5020-08500 (£97)


lc columns inertsil - hichrom · isolated silanol si-oh 'eminalsilanol3i /() 2 silica-gel...

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