Light

Dark

WT veA1

OE:: rsmA veA1

∆laeA veA1

OE:: rsmA ∆laeAveA1

ST

Supplementary Figure 1. Thin layer chromatography of chloroform extracts from WT, OE::rsmAveA1, ∆laeAveA1 and OE::rsmA∆laeAveA1 (RDIT2.3, RMS11.1, RJW46.4 and RMS53.1 respectively) at dark and light. OE::rsmAveA1 has high impact on ST and secondary metabolite production with remediation of ST production of ∆laeA mutant either at dark or light. (ST: sterigmatocystin standard).

Light

Dark

WT veA

OE:: rsmA veA

∆laeA veA

OE:: rsmA ∆laeAveAST

Supplementary Figure 2.Thin layer chromatography of chloroform extracts from wild type (WT), OE::rsmA, ∆laeAveA and OE::rsmA∆laeAveA (RDIT9.32, RMS4.25, RJW41.A and RMS41.1) respectively. OE::rsmAveA has greatly enhanced ST and secondary metabolite production with remediation of ST production of ∆laeA mutant at dark. (ST: sterigmatocystin standard).

OE::rsmA ∆veA∆veA

ST

DarkLight

OE::rsmA ∆veA∆veA ST

Supplementary Figure 3.Thin layer chromatography of chloroform extracts of ST production by ∆veA and OE::rsmA∆veA (RJW112.2 and RMS4.1696 respectively) from solid GMM. OE::rsmA∆veA has greatly enhanced ST and secondary metabolite production with remediation of ST production of ∆veA mutant at both dark and light. (ST: sterigmatocystin standard).

Day 7 - Champes

0

500000000

1000000000

1500000000

2000000000

2500000000

WT

laeA

, veA

OE::rsm

A, veA

OE::rsm

A, lae

A, veA

WT

laeA

, veA

OE::rsm

A, veA

OE::rsm

A, lae

A, veA

Sex

ual

sp

ore

s/m

m2

a

b

a

a

bb c c

Day 7 - GMM

0.00E+00

5.00E+06

1.00E+07

1.50E+07

2.00E+07

2.50E+07

3.00E+07

3.50E+07

4.00E+07

4.50E+07

5.00E+07

WT laeA,veA

OE::rsmA,

veA

OE::rsmA,

laeA,veA

WT laeA,veA

OE::rsmA,

veA

OE::rsmA,

laeA,veA

Sex

ual

sp

ore

s/m

m2

Light

Dark

c

a

b

a

babb

a

Supplementary Figure 4.Overexpression of rsmA does not remediate sexual defects in laeA. Sexual development on Champes or GMM media. The laeA mutant produces the same number of ascopores in dark and light regimes when grown on Champes medium. WT (RDIT9.32), laeA,veA (RJW41.A), OE::rsmA, veA (RMS4.25), OE::rsmA,laeA,veA (RMS41.1). Media was inoculated with 1X106 spores and incubated for 7 days in the light and dark. Error bars designate standard deviations. Letters (a-c) indicate differences between strains within a light regime (e.g. light on Champes medium) with statistical significance (P< 0.05) according to the Tukey-Kramer multiple comparison test.