lightbulb pcr arduino code
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TRANSCRIPT
// Arduino code to run a pcr machine with a heating element, a cooling element and a thermistor//This code is still very much in the editing stage, current as of January 6th 2010//Written by Russell Durrett, [email protected] int thermpin = A0;const int heatpin = 3;const int coolpin = 9;const int hot = 40; //these are resistance-based temperatures, not yet calibrated for Celsiusconst int tm = 20;const int elongtemp = 30;const int delaytime = 2000;const int elongtime = 5000;int cyclecount = 1;void setup(){ pinMode(thermpin, INPUT); pinMode(heatpin, OUTPUT); pinMode(coolpin, OUTPUT); analogReference(EXTERNAL); Serial.begin(9600);}void loop(){ int temp = analogRead(thermpin);do { //Begin PCR Reaction by heating to 95 degrees; digitalWrite(heatpin, HIGH); temp = analogRead(thermpin); Serial.print("Cycle #"); Serial.print(cyclecount); Serial.print(", Heating Lamp ON, Temp = "); Serial.println(temp); } while (temp < hot);digitalWrite(heatpin, LOW);Serial.println("DNA Dissociating");delay(delaytime);do { digitalWrite(coolpin, HIGH); temp = analogRead(thermpin); Serial.print("Cycle #"); Serial.print(cyclecount); Serial.print(", Cooling to Tm, Temp = "); Serial.println(temp);} while (temp > tm);digitalWrite(coolpin, LOW);Serial.println("Primers Annealing");delay(delaytime);do{ digitalWrite(heatpin, HIGH); temp = analogRead(thermpin); Serial.print("Cycle #"); Serial.print(cyclecount); Serial.print(", Heating to Extension Temp, Temp = "); Serial.println(temp);} while (temp < elongtemp);digitalWrite(heatpin, LOW); Serial.print("Cycle #"); Serial.print(cyclecount);Serial.println(", DNA Elongating");delay(elongtime);//strobe bulb to maintain elongtemp?cyclecount++;//digitalWrite(cyclespin, cyclesdonecount); }