martin j. cann signal transduction mediated by inorganic ions
DESCRIPTION
The major biologically active inorganic ions Although the physiology of the predominant inorganic ions is well understood, sensor mechanisms remain elusive. Cations Na + ? K + ? Anions Cl - guanylyl cyclase-A HCO 3 - sAC, Spirulina CyaCTRANSCRIPT
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Martin J. Cann
Signal transduction mediated by
inorganic ions.
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The importance of the major biologically activeinorganic ions.
pH homeostasisVolume homeostasisSolute transportAction potentialsGas transportFluid secretion
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The major biologically active inorganic ions
Although the physiology of the predominant inorganicions is well understood, sensor mechanisms remainelusive.
CationsNa+ ?K+ ?
AnionsCl- guanylyl cyclase-AHCO3
- sAC, Spirulina CyaC
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Anabaena strain PCC7120
Why Anabaena strain PCC7120?• Clear role for inorganic ions in organismal biology• Genome is sequenced• Wild type and mutant strains are available
What do we hope to learn?1) What are the phenotypic consequences of the loss of inorganic
ion responsive genes?2) What insights can recombinant protein provide us regarding
the mechanism of enzyme activation by inorganic ions?
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HCO3- responsive adenylyl cyclases
in prokaryotes and eukaryotes.
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Biological functions for HCO3
Gas exchangepH homeostasisSperm maturationNucleotide synthesisCarbon fixation
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Class I - Enterobacteriacae
Class II - Toxin producing eubacteria
Class III - ‘Universal’ Class, mammals and some prokaryotes
Class IV - Aeromonas hydrophila
Class V - Prevotella ruminicola
Class VI - Genomes of Rhizobiaceae
HCO3- responsive
e.g. mammalian sAC,Spirulina CyaC
HCO3- non-responsive
e.g. mammalian tmAC
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GAF-A GAF-B ACPASN C
G595K465I394L385L237T207Q50 K859
CyaB1595-859
CyaB1
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100
150
200
250
300
350
400
Bas
al
NaC
l
KC
l
NaH
CO
3
KH
CO
3
0
Spec
ific
Act
ivity
[nm
ol c
AM
P/m
g/m
in]
GAF-A GAF-B ACPASN C
CyaB1595-859
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0 0.1 1.0 10.0 100.00
100
200
300
400
Salt [log mM]
Spec
ific
Act
ivity
[nm
ol c
AM
P/m
g/m
in]
GAF-A GAF-B ACPASN C
CyaB1595-859
NaHCO3
NaCl
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1.11.1Hill coefficient
97.73.7
91.64.9Ea (kJ/mol)
3.5 x 103
3.7 x 103
7.02.6kcat (min-1)
238.036.3
93.58.2
Vmax(nmol/mg/min)
33.32.8
11.80.8KM (ATP) (M)
HCO3-Cl-
kcat/KM(M-1 sec-1)
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0 2 4 6 8 100
100
200
300
Acc
umul
ated
cA
MP
[pm
ol/a
ssay
]
Time [mins]
GAF-A GAF-B ACPASN C
+
+
CyaB11-859
KHCO3
KCl
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Anabaena CyaB1 638 FNYEGTLDKFIGDALM (59) GSHKRMDYTVIGDGVN---LSSRLETV 736Rattus sAC C1 87 LIFGGDILKFAGDALL (55) GDETRNYFLVIGQAVDDVRLAQNMAQM 184Rattus sAC C2 336 FMFD------KGCSFL (51) GHTVRHEYTVIGQKVN---IAARMMMY 420Spirulina CyaC 1049 FENQGTVDKFVGDAIM (66) GSQERSDFTAIGPSVN---IAARLQEA 1154Stigmatella CyaB8 203 LTCGGTLDKFLGDGLM (66) GGSMRTEYTCIGDAVN---VAARLCAL 308Mycobacterium Rv1319 399 DRHHGLINKFAGDAAL (50) GAKQRFEYTVVGKPVN---QAARLCEL 488Mycobacterium Rv1264 253 TAPPVWFIKTIGDAVM (40) -----RAGDWFGSPVN---VASRVTGV 327Bos AC1 C1 345 HCR---RIKILGDCYY (54) GLR-KWQYDVWSNDVT---LANVMEAA 434Bos AC1 C2 915 FYKDLEKIKTIGSTYM (62) GAR-RPQYDIWGNTVN---VASRMDST 1015Rattus AC3 C1 359 HQL---RIKILGDCYY (54) GQK-RWQYDVWSTDVT---VANKMEAG 448Rattus AC3 C2 967 KFRVITKIKTIGSTYM (72) GAR-KPHYDIWGNTVN---VASRMEST 1077Mus AC9 C1 434 KCE---KISTLGDCYY (54) GMR-RFKFDVWSNDVN---LANLMEQL 519Mus AC9 C2 1096 DYNSIEKIKTIGATYM (62) GTT-KLLYDIWGDTVN---IASRMDTT 1196Rattus GCA 912 DVY---KVETIGDAYM (57) GLK-MPRYCLFGDTVN---TASRMESN 1004
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50
100
150
200
250
1 10 1000Salt [log mM]
Spec
ific
Act
ivity
[nm
ol c
AM
P/m
g/m
in]
01 10 100
0
2.5
5.0
7.5
10.0
Salt [log mM]
Spec
ific
Act
ivity
[nm
ol c
AM
P/m
g/m
in]
0
GAF-A GAF-B ACPASN C
CyaB1595-859
CyaB1595-859 CyaB1595-859 K646A
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V722
NH2
N
N
O
1
O
O
NH3+K646
HO C
Anabaena CyaB1
I1019
NH2
N
N
O
1
O
O
NH3+
D1018
K938
VC1·IIC2
C
6
6
N
N
N
N
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1 10 1000
5
10
15
20
Salt [log mM]
Spec
ific
Act
ivity
[nm
ol c
AM
P/m
g/m
in]
0 0Salt [log mM]
Spec
ific
Act
ivity
[nm
ol c
AM
P/m
g/m
in]
1 10 1000
2.5
5.0
7.5
10.0
12.5
15.0
Mycobacterium tuberculosisRV1319c Thr
Mycobacterium tuberculosisRV1264 Asp
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Mechanism of HCO3- activation of AC
• HCO3- increases rate of substrate turnover.
• HCO3
- is hypothesized to be co-ordinated in the active site by an essential lysine residue.
• HCO3- is hypothesized to mimic a carboxy group.
• A Thr/Asp polymorphism can be used as a predictor of HCO3
- responsiveness.
• HCO3- responsive ACs can be detected in the
genomes of many prokaryotes and several eukaryotes.
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GAF domain mediatedNa sensing.
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Biological functions for Na
pH homeostasisMaintenance of blood pressureAction potentialsSolute transportVolume homeostasis
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0 2 4 6 8 100
100
200
300
Acc
umul
ated
cA
MP
[pm
ol/a
ssay
]
Time [mins]
GAF-A GAF-B ACPASN C
+
+
CyaB11-859
KHCO3
KCl
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0
50
100
150
200
250
300pm
ol c
AM
P/as
say
Rb CsLi KNaNone
GAF-A GAF-B ACPASN C
CyaB11-859
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0 1 10 1000
100
200
300
Salt [mM]
pmol
cA
MP/
assa
y
GAF-A GAF-B ACPASN C
CyaB11-859
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0 1 2 3 4 5 6 7 8 9 10
0
1
250 mM NaCl0 mM NaCl
Time [mins]
nmol
cA
MP/
assa
y
GAF-A GAF-B ACPASN C
+
+
CyaB11-859
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0
0
250
500
750
10000 mM NaCl
50 mM NaCl
-7 -6 -5cAMP log [M]
pmol
cA
MP/
assa
y
GAF-A GAF-B ACPASN C
+
+
CyaB11-859
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0
50
100
150
200
250
1 2 3 4 5 6
pmol
cA
MP/
assa
y
+Na +Na +Na
CyaB1 1-859 CyaB1 1-859 D190A GAF-A
CyaB1 1-859 D360A GAF-B
GAF-A GAF-B ACPASN C
+
+
CyaB11-859
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Anabaena sp. PCC7120
WT cyaB1
BG11
BG11/ 40 mM NaCl
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3.01.00.80.60.40.2
Wild type
cyaB1
NaCl [mM]
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0
0.01
0.02
0.03
0.04
Gro
wth
Rat
e[
/hr-1
]
Anabaena sp. PCC7120WT
Anabaena sp. PCC7120cyaB1
0 4 40NaCl [mM]
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0.20.2 4.04.0
NaCl [mM]
pH
7.0
8.5
9.0
7.5
8.0
WT cyaB1
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-50000
-40000
-30000
-20000
-10000
0
0 100 200 300 400 500 600
Time [secs]
WTcyaB1
Fl
uore
scen
ce In
tens
ity [A
rbitr
ary
Uni
ts] cells
Na
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H2O + CO2 HCO3- + H+
H+
CA
Sym
HCO3- Na+
out
in
A
-9
-8
-7
-6
-5
-4
-3
-2
-1
0
1
2
0 100 200 300time [secs]
WTcyaB1
H
+ [pm
ol/
g ch
loro
phyl
l]
Light off
Light on
A
B
Ant
H+
Na+
out
in
B
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4 mM Na+
CyaB1
out
in
CyaB1
cAMP
0.2 mM Na+
out
in
Na/HAnt
Na+
H+
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GAF domain mediated Na sensing
• CyaB1 is the first identified Na sensor.• GAF domains are found throughout the animal, plant, and microbial kingdoms.• GAF domains may mediate at least some aspects of Na detection in diverse organisms.
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The major biologically active inorganic ionsCationsNa+ GAF domain of Anabaena CyaB1K+ ?
AnionsCl- guanylyl cyclase-AHCO3
- A defined subset of Class III adenylylcyclases
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University of TübingenJoachim SchultzTobias KanacherJurgen Linder
University of DurhamMartin CannArne HammerJie Zhou
University of TokyoMasayuki Ohmori