Mass Mass SpectrometerSpectrometerAuto SamplerAuto Sampler

Kyle SalaJake Ahrens

Stephen PearsonSeth Yellin

Project SummaryProject Summary• Develop an auto sampling device to interface with

a mass spectrometer• Samples involve bacterial pathogens to be

recognized and catalogued by the mass spectrometer

Where We’re At…Where We’re At…• No Significant Design Changes• Valve System Construction

o Solenoid Valveso Tubingo Incubator

• Valve Code• Full interface capabilites with Mass Spectrometer

will be left for future CS students• Magnetic Stirrer

o As requested by our faculty advisor, is no longer a parameter of our project

Valve System Valve System ConstructionConstruction

…Previously……Previously…• Did not have all the parts (tubing, valves,

connectors…)• Recently obtained the incubator

o Temperature needs to stay constant in the samples in order to secure consistent readings

• Had not begun construction

Valve System Valve System ConstructionConstruction

…Now……Now…• Completed• Valves are mounted in the incubator and

connected in series via tubing system• Bottles are prepped and ready to be filled with

samples and incorporated into the system

6

7

Valve CodeValve Code…Previously……Previously…

• Coding – pseudo code for all operations of valve systemo Sampling, Flushing, CO2 Removal, Air Replacement

• Two independent microcontrollers• Extended pseudo code to control new chip

o Quad High Side Driver

Valve CodeValve Code…Now……Now…

• Completed• Two linked microcontrollers• ‘Sequence functions’

o Flushingo Samplingo Replace headspace

• Special thanks to professor Titcomb

11

TestingTesting• Valve system has to be air tight

o Valves tested with pressure gauges in lab

• Preview video on how our code operates the valves

Interface with Mass Interface with Mass SpectrometerSpectrometer

• Extension for future CS students

• Have our code interactwith mass spectrometersoftware through sampleacquisition

• Name and categorizeeach pathogen as itssampled

15

Interface with Mass Interface with Mass SpectrometerSpectrometer

• Mass spec uses Analyst software from Applied Biosystems

• Contacted software support for instructions on interfacing with autosamplers

• The software cannot directly connect to our valving system but can connect to other windows programs that can

Looking ForwardLooking Forward• Solder valve connections for code implementation• Test and trouble shoot

o “Bubble” presentation of system Film next week

• Make a complete “instruction manual” for future reference

• Final assembly of power supply and external controls

• Making it look cool

Questions?Questions?