masterplex qt: luminex multiplex quantitative analysis software
DESCRIPTION
MasterPlex QT is a multiplex curve-fitting analysis software for the Luminex xMAP & Bio-Plex platforms.This presentation accompanies the following free webinar: https://www1.gotomeeting.com/register/351125992The webinar begins with a close-up look at the 4 Parameter Logistic (4PL) and 5 Parameter Logistic (5PL) nonlinear regression models. Then it goes into how Luminex xMAP/Bio-Plex users can improve their results with MasterPlex QT and covers some of the following topics:- Heteroscedasticity & Weighting- BONUS: What’s New in MasterPlex QT 2010- Ensuring the quality of your data with the Quality Control Manager- How to use time-saving features such as templates & Best FitMasterPlex QT product page: http://www.miraibio.com/qtTRANSCRIPT
© Hitachi Software Engineering America, Ltd. 2010. All rights reserved.
Presenters: Robert LyndeDeputy DirectorSales Operations & Strategic Alliances Manager
Allen LiuSupervisorTechnical Support & Project Management
Webinar: Improving your results with MasterPlex QT
July 29, 2010
This presentation accompanies the free webinar recording at:https://www1.gotomeeting.com/register/351125992
© Hitachi Software Engineering America, Ltd. 2010. All rights reserved.
1) A close up look at the 4-PL and 5-PL model equations2) Heteroscedasticity & Weighting3) BONUS: What’s New in MasterPlex QT 20104) Ensuring the quality of your data with the Quality Control
Manager5) How to use time-saving features such as templates & Best Fit
Topics
© Hitachi America, Ltd. 2010. All rights reserved.
4 Parameter Logistic (4PL)
• F(x) = ((A-D)/(1+((x/C)^B))) + D
• Nonlinear regression
• Monotonic– e.g. For every MFI, there is only one
concentration
• Symmetrical around the midpoint or inflection point
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4 Parameter Logistic (4PL)
A = minimum asymptote
D = maximum asymptote
C = inflection pointB = slope factor/steepness of curve
< low standard OR lowest extrapolated value
> high standard OR highest extrapolated value
© Hitachi America, Ltd. 2010. All rights reserved.
Caution on Extrapolation
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tiny change in MFI = HUGE change in concentration
© Hitachi America, Ltd. 2010. All rights reserved.
5 Parameter Logistic (5PL)
• F(x) = A + (D/(1+(X/C)^B)^E)
• Nonlinear regression
• Monotonic
• Key difference = asymmetry
• E = asymmetry factor
• E = 1 = 4PL model equation
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© Hitachi America, Ltd. 2010. All rights reserved.
5 Parameter Logistic (5PL)
© Hitachi America, Ltd. 2010. All rights reserved.
Assumptions of the 4PL & 5PL
• 2 assumptions about how the data are scattered around the curve…1. The scatter follows a Gaussian or “normal”
distribution
2. The standard deviation of the scatter (of the response values or MFI) is the same for all values of x (or concentrations in our case)
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© Hitachi America, Ltd. 2010. All rights reserved.
Heteroscedasticity
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© Hitachi America, Ltd. 2010. All rights reserved.
Weighting
• 1/Y^2 (Relative weighting) – Most commonly used
• 1/Y – Most effective when the scatter follows a Poisson distribution
• Mainly affects points on the lower portion of the curve
• Test by doing before/after on the % Recovery
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© Hitachi Software Engineering America, Ltd. 2010. All rights reserved.
• Expanded Platform Support• Meso Scale Discovery (MSD) platform compatibility
• Improved Data Integrity• Built-in Quality Control Manager• Added new Upper Limit of Detection calculation for QC
Manager• Further Time-saving
• Auto calculation for plate templates• Dose Response Analysis
• EC or IC50 calculation • EC or IC(anything) calculation
• Enhanced Reporting• Merge group wells in the Data Table• Customizable standard curve chart titles & x/y-axes
What’s new for MasterPlex QT 2010?
© Hitachi Software Engineering America, Ltd. 2010. All rights reserved.
• Free 14-Day Trial: http://www.MiraiBio.com/QT• Support: [email protected]• Sales: [email protected]• MiraiBio Support Menu
• Community• Knowledge Base• Blog
• Webinars: http://www.MiraiBio.com/Webinars
Resources
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