MC1227

p-Acetamidophenol, [ring-14C(U)]-

Lot 198-185-079-A-20080611-DRE

A) All chromatograms were run using the HPLC method described on the Product Data Sheet.

Concentrations and volumes:

Standard solution concentration was 1.0 mg/mL.

p-Acetamidophenol, [ring-14C(U)]- concentration was 94.0 µCi/mL. Volume of standard alone injection was 1.0 µL.

Volume of p-Acetamidophenol, [ring-14C(U)]- alone injection was 1.0 µL.

Co-injection solution consisted of 1.0 µL p-Acetamidophenol, [ring-14C(U)]- + 1.0 µL standard.

Volume of co-injection was 2.0 µL.

Volume of blank injection was 1.0 µL.

B) Mass spectrometry- Positive Mode C) NMR

MC1227p-Acetamidophenol, [ring-14C(U)]-Lot 198-185-079-A-20080611-DRE

Chromatogram Info:

File Name : L:\int31241 File Created : 11/6/2013 9:29:18 AM

Origin : Acquired, Acquisition started 6/15/2012 3:21:15 PM Acquired Date : 6/15/2012 4:01:14 PM

Project : Test By : Administrator

Method : Unit3-40minrun By : Administrator

Description : Radiochemical trace of 14C material alone

Created : 6/12/2008 10:29 AM Modified : 11/6/2013 10:30 AM

Column : Detection : Radiochemical

Mobile Phase : Temperature :

Flow Rate : Pressure :

Note :

[min.]Time

10 20 30 40

[V]

Volta

ge

0.0

0.2

0.4

0.6

0.8

1.0

17.8

18

.5

18.9

21.6

22

.1

22.8

L:\ int 31241 - Unit 3 Radio

11/6/2013 10:31 AM Page 1 of 2Chromatogram L:\int31241.PRM

Result Table (Uncal - L:\int31241 - Unit 3 Radio)

CompoundName

Reten. Time[min]

Area[%]

Area[mV.s]

Height[mV]

Height[%]

Efficiency[th.pl]

1 17.813 0.06 12.019 0.954 0.104 34215.653

2 18.530 0.03 6.337 0.608 0.066 54591.755

3 18.870 99.55 20225.927 912.305 99.438 24004.860

4 21.583 0.06 11.612 0.415 0.045 526684.722

5 22.100 0.01 1.333 0.347 0.038 460342.582

6 22.800 0.30 60.809 2.833 0.309 44874.000

Total 100.00 20318.035 917.462 100.000

11/6/2013 10:31 AM Page 2 of 2Chromatogram L:\int31241.PRM

MC1227p-Acetamidophenol, [ring-14C(U)]-Lot 198-185-079-A-20080611-DRE

Chromatogram Info:

File Name : L:\int31241 File Created : 11/6/2013 9:29:18 AM

Origin : Acquired, Acquisition started 6/15/2012 3:21:15 PM Acquired Date : 6/15/2012 4:01:14 PM

Project : Test By : Administrator

Method : Unit3-40minrun By : Administrator

Description : UV trace of 14C material alone

Created : 6/12/2008 10:29 AM Modified : 11/6/2013 10:31 AM

Column : Detection : UV 255nm

Mobile Phase : Temperature :

Flow Rate : Pressure :

Note :

[min.]Time

10 20 30 40

[mV]

Volta

ge

0

50

100

150

18.5

22.4

L:\ int 31241 - Unit 3 UV

11/6/2013 10:31 AM Page 1 of 2Chromatogram L:\int31241.PRM

Result Table (Uncal - L:\int31241 - Unit 3 UV)

CompoundName

Reten. Time[min]

Area[%]

Area[mV.s]

Height[mV]

Height[%]

Efficiency[th.pl]

1 18.547 99.68 625.834 136.789 99.696 388906.694

2 22.437 0.32 2.013 0.418 0.304 474474.934

Total 100.00 627.847 137.207 100.000

11/6/2013 10:31 AM Page 2 of 2Chromatogram L:\int31241.PRM

MC1227p-Acetamidophenol, [ring-14C(U)]-Lot 198-185-079-A-20080611-DRE

Chromatogram Info:

File Name : L:\int31243 File Created : 11/6/2013 9:29:18 AM

Origin : Acquired, Acquisition started 6/15/2012 5:16:19 PM Acquired Date : 6/15/2012 5:56:18 PM

Project : Test By : Administrator

Method : Unit3-40minrun By : Administrator

Description : UV trace of standard alone

Created : 6/12/2008 10:29 AM Modified : 11/6/2013 9:33 AM

Column : Detection : UV 255nm

Mobile Phase : Temperature :

Flow Rate : Pressure :

Note :

[min.]Time

10 20 30 40

[mV]

Volta

ge

0

200

400

600

800

18.6

L:\ int 31243 - Unit 3 UV

11/6/2013 10:32 AM Page 1 of 2Chromatogram L:\int31243.PRM

Result Table (Uncal - L:\int31243 - Unit 3 UV)

CompoundName

Reten. Time[min]

Area[%]

Area[mV.s]

Height[mV]

Height[%]

Efficiency[th.pl]

1 18.627 100.00 3292.044 699.594 100.000 392268.984

Total 100.00 3292.044 699.594 100.000

11/6/2013 10:32 AM Page 2 of 2Chromatogram L:\int31243.PRM

MC1227p-Acetamidophenol, [ring-14C(U)]-Lot 198-185-079-A-20080611-DRE

Chromatogram Info:

File Name : L:\int31244 File Created : 11/6/2013 9:29:19 AM

Origin : Acquired, Acquisition started 6/18/2012 9:57:33 AM Acquired Date : 6/18/2012 10:37:32 AM

Project : Test By : Administrator

Method : Unit3-40minrun By : Administrator

Description : Radiochemical trace of 14C material co-injected with standard

Created : 6/12/2008 10:29 AM Modified : 11/6/2013 10:33 AM

Column : Detection : Radiochemical

Mobile Phase : Temperature :

Flow Rate : Pressure :

Note :

[min.]Time

10 20 30 40

[V]

Volta

ge

0.0

0.2

0.4

0.6

0.8

1.0

17.7

18.8

20.6

22.5

22

.8

27.4

L:\ int 31244 - Unit 3 Radio

11/6/2013 10:34 AM Page 1 of 2Chromatogram L:\int31244.PRM

Result Table (Uncal - L:\int31244 - Unit 3 Radio)

CompoundName

Reten. Time[min]

Area[%]

Area[mV.s]

Height[mV]

Height[%]

Efficiency[th.pl]

1 17.663 0.04 9.669 1.026 0.105 67517.310

2 18.783 99.46 23615.341 974.408 99.319 21717.638

3 20.650 0.08 19.864 0.527 0.054 291651.932

4 22.483 0.05 11.435 0.987 0.101 52938.970

5 22.817 0.24 57.222 3.020 0.308 37679.097

6 27.370 0.12 29.446 1.120 0.114 19900.351

Total 100.00 23742.978 981.088 100.000

11/6/2013 10:34 AM Page 2 of 2Chromatogram L:\int31244.PRM

MC1227p-Acetamidophenol, [ring-14C(U)]-Lot 198-185-079-A-20080611-DRE

Chromatogram Info:

File Name : L:\int31244 File Created : 11/6/2013 9:29:19 AM

Origin : Acquired, Acquisition started 6/18/2012 9:57:33 AM Acquired Date : 6/18/2012 10:37:32 AM

Project : Test By : Administrator

Method : Unit3-40minrun By : Administrator

Description : UV trace of 14C material co-injected with standard

Created : 6/12/2008 10:29 AM Modified : 11/6/2013 10:29 AM

Column : Detection : UV 255nm

Mobile Phase : Temperature :

Flow Rate : Pressure :

Note :

[min.]Time

10 20 30 40

[mV]

Volta

ge

0

200

400

600

800

18.4

19.5

19

.9

L:\ int 31244 - Unit 3 UV

11/6/2013 10:33 AM Page 1 of 2Chromatogram L:\int31244.PRM

Result Table (Uncal - L:\int31244 - Unit 3 UV)

CompoundName

Reten. Time[min]

Area[%]

Area[mV.s]

Height[mV]

Height[%]

Efficiency[th.pl]

1 18.427 99.56 4134.716 832.914 99.616 349784.428

2 19.520 0.21 8.731 1.901 0.227 474954.394

3 19.893 0.23 9.452 1.312 0.157 205326.028

Total 100.00 4152.898 836.126 100.000

11/6/2013 10:33 AM Page 2 of 2Chromatogram L:\int31244.PRM

MC1227p-Acetamidophenol, [ring-14C(U)]-Lot 198-185-079-A-20080611-DRE

Chromatogram Info:

File Name : L:\int31240 File Created : 11/6/2013 9:29:18 AM

Origin : Acquired, Acquisition started 6/15/2012 2:29:57 PM Acquired Date : 6/15/2012 3:09:56 PM

Project : Test By : Administrator

Method : Unit3-40minrun By : Administrator

Description : UV trace of blank injection

Created : 6/12/2008 10:29 AM Modified : 11/6/2013 9:30 AM

Column : Detection : UV 255nm

Mobile Phase : Temperature :

Flow Rate : Pressure :

Note :

[min.]Time

10 20 30 40

[mV]

Volta

ge

-10

0

10

20

30

L:\ int 31240 - Unit 3 UV

11/6/2013 10:34 AM Page 1 of 2Chromatogram L:\int31240.PRM

Result Table (Uncal - L:\int31240 - Unit 3 UV)

CompoundName

Reten. Time[min]

Area[%]

Area[mV.s]

Height[mV]

Height[%]

Efficiency[th.pl]

No peak to report

11/6/2013 10:34 AM Page 2 of 2Chromatogram L:\int31240.PRM

C:\Xcalibur\data\MC1227-20080611-DRE 6/15/2012 4:05:41 PM MC1227-20080611-DRE

RT: 0.00 - 9.00

0 1 2 3 4 5 6 7 8 9

Time (min)

0

5

10

15

20

25

30

35

40

45

50

55

60

65

70

75

80

85

90

95

100

Relative Abundance

4.77

0.484.59

4.183.860.76

3.53

3.30

2.62

2.32

2.16

NL:8.25E8

TIC F: MS MC1227-20080611-DRE

MC1227-20080611-DRE#31-70 RT: 0.39-0.89T: + c sid=-10.00 Q3MS [ 50.00-400.00]m/z= 189.60-214.65

m/z Intensity Relativem/z Intensity Relative

190.03 132798.1 0.25 192.27 132310.2 0.25 193.04 33481495.5 62.45 194.04 3831888.4 7.15 195.04 53609525.1 100.00 196.04 5721353.2 10.67 197.04 37235822.3 69.46 198.04 4621125.3 8.62 199.05 14375981.9 26.82 200.05 2248234.8 4.19 201.05 3236584.1 6.04 202.05 920277.7 1.72 203.05 509338.1 0.95 204.07 294611.2 0.55 208.00 126732.5 0.24 210.06 241195.5 0.45 211.03 134090.8 0.25 212.04 804222.2 1.50 213.06 257792.4 0.48 214.04 477326.6 0.89

MC1227-20080611-DRE #31-72 RT: 0.39-0.91 AV: 42 NL: 7.79E7T: + c sid=-10.00 Q3MS [ 50.00-400.00]

50 100 150 200 250 300 350 400

m/z

0

5

10

15

20

25

30

35

40

45

50

55

60

65

70

75

80

85

90

95

100

Relative Abundance

129.07

195.04

154.0588.11

83.08

307.00

321.96217.01 261.69396.97

MC1227-20080611-DRE #31-68 RT: 0.39-0.86 AV: 38 NL: 5.40E7T: + c sid=-10.00 Q3MS [ 50.00-400.00]

190 200 210 220 230 240 250

m/z

0

5

10

15

20

25

30

35

40

45

50

55

60

65

70

75

80

85

90

95

Relative Abundance

195.04

197.04

193.04

199.05

201.05217.01 242.18

235.97202.05 227.09186.15 251.05

98

76

54

32

1pp

m

2.094

6.7226.7456.751

7.2997.329

2.98

2.09

2.00

PC 1.00

GB 0

LB 0.00 Hz

SSB 0

WDW no

SF 300.1300000 MHz

SI 32768

SFO1 300.1318534 MHz

PL1 0.00 dB

P1 7.25 usec

NUC1 1H

======== CHANNEL f1 ========

TD0 1

D1 1.00000000 sec

TE 300.0 K

DE 6.50 usec

DW 92.400 usec

RG 645.1

AQ 6.0555763 sec

FIDRES 0.082569 Hz

SWH 5411.255 Hz

DS 2

NS 512

SOLVENT MeOD

TD 65536

PULPROG zg30

PROBHD 5 mm DUX 3H−1H

INSTRUM spect

Time 10.25

Date_ 20120712

PROCNO 1

EXPNO 1

NAME MC1227−20080611−DRE

Batch 20080611−DRE

MC1227 1H NMR in MeOD