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MICROBIOLOGIA GENERALE
Microbial evolution and systematics
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Microbial evolution and systematics:
conventional and molecular taxonomy
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Example of methods that wouldbe used for identification of a
newly isolated enteric bacterium, using classic microbiological methods
Conventional taxonomy
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Ranges of GC ratios of various organisms
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Genomic hybridization as a taxonomic tool
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Genomic hybridization as a taxonomic tool
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Ribotyping
Multilocus sequence typing (MLST)
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Fatty acid methyl ester (FAME) analysis in bacterial identification: classes of fatty acids in Bacteria.
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Fatty acid methyl ester (FAME) analysis in bacterial identification
Each peak from the gas chromatograph is due to one particular fatty acid methyl ester and the peak height is proportional to the amount.
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Microbial evolution and systematics:
evolutionary chronometers and microbial philogeny
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Escherichia coli16S RNA
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Escherichia coli 16S RNA
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The polymerase chain reaction (PCR)
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Ribosomal RNA sequencing of a pure culture of a microorganism
using the PCR
16S-ribosomal RNA geneslocated on the E. coligenome amplified by PCR
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Sanger ddNTP chain terminator sequencing
G A T C
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Preparing a phylogeneticdistance tree
rom 16SrRNA sequences
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Bacillus megaterium
Saccharomyces cerevisiae
Nitrobacter spp.
Nitrosomonas spp.
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Steps in biodiversity analysisof a microbial communityusing phylogenetic trees
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Universal phylogenetic tree as determined from comparative rRNA sequencing
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Microbial evolution and systematics:
unique phenotypic characteristics of the primary domains
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1: Cell walls
•Virtually all Bacteria have cell walls containing peptidoglycan.•Peptidoglygan can thus be considered a signature for Bacteria
•Eukarya and Archaea lack peptidoglycan.
•In Archaea various cell wall types exist: pseudopeptidoglycan, polysaccharide, protein, or glycoprotein
•In Eukarya when present cell walls are made of cellulose or chitin
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2: Plasma membrane lipids
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3: RNA polymerases
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Microbial evolution and systematics:
the specie concept in microbiology and
nomenclature
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Relationship between 16S ribosomal RNA sequence similarity and genomic DNA hybridization between different pairs of organisms
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Prokaryotic nomenclature: the taxonomic hierarchy DOMAIN
PHYLUM
KINGDOM
CLASS
ORDER
FAMILY
GENUS
SPECIESEscherichia coli
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Possible mechanism of bacterial speciation
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Microbial evolution and systematics:
primitive microorganisms
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Major landmarks inbiological evolution
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Possible scenario for the evolution of cellular life forms from RNA life forms
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A hypothetical energy-generating scheme for primitive cells
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Origin of modern eukaryotesby endosymbiotic events
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Major landmarks inbiological evolution