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Microbiology Practical I

The following slides were contributedby your fellow BIO 251 students

Use side key to view slides

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Lab 1: Eukaryotes and Microscopy

Know the parts

of themicroscope

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Lab 1: Eukaryotes and Microscopy

Paramecium caudatum

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Lab 1: Eukaryotes and Microscopy

Euglena gracilis

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Lab 1: Eukaryotes and Microscopy

Amoeba proteus

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Lab 1: Eukaryotes and Microscopy

Fungus

Saccharomyces cerevisiae

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Lab 1: Eukaryotes and Microscopy

Cyanobacteria ~ Nostoc

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~Bacteria slides~

Micrococcus luteus

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~Bacteria Slides~

Bacillus subtilis

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~Bacteria Slides~

Bacillus anthracis

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~Bacteria Slides~

Rhodospirillum rubrumSorry not in focus

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~Bacteria Slides~

See other file for Gram Stain, Endospore, Acidfast,and Capsule Staining

[Adobe Acrobat of Gram and Endospore]

Some Endospore Staining ~

http://localhost/var/www/apps/conversion/current/tmp/scratch21538/More_microscopic_slides_of_bacteria.pdfhttp://localhost/var/www/apps/conversion/current/tmp/scratch21538/More_microscopic_slides_of_bacteria.pdf

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Streak Plate:

Know colony characteristics (color, elevation, margin, etc.)

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Spread Plate vs. Streak Plate:

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Streak Plate:

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Spread Plate:MixedBacillus subtilis & Serratia marcescens

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Practical I Unknown Tests:

~ Simple Stain

~ Gram Stain

~ Endospore Stain

~ Acid-Fast Stain (Demo)

~ Capsule Stain (Demo)

~ Motility (Hanging Drop, Wet Mount, Soft Agar Deep)

~ Obligate Aerobe, Facultative Anaerobe,Aerotolerant Anaerobes, Microaerophiles(Gas Pak, Eugon Agar, Thioglycollate Broth)

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Gas Pak Anaerobic vs. Aerobic:

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Dilution Problems:

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Dilution Problems:

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Bacterial Growth Curve:

Make sure you knowhow to:

Use a

spectrophotometer

to measure

absorbance.

Produce a log graph

of the calculations.

Calculate generation

time from a growth

curve.