microscope directions for advanced biology 9th grade
TRANSCRIPT
Today
Compound Light Microscope• Examine and diagram at least 1 each:
• plant cell• To do: elodea, onion
• animal cell • To do: your own cheek cells
• bacterial cell• To do: Prepared slide
elodea onion
cheek bacteria
Tips for Producing High-quality Microscope Drawings Name: Class: Period: Date: Phylum: Sarcodina (Drawing & labeling: 31/2 points)
Title: An Amoeba Eating Another Protist
Genus: Amoeba Observations: The amoeba was a single cell and seemed to move by oozing over the surface. It was the largest organism on the slide. It would push different parts of its body along to try and catch other organisms. One Paramecium got stuck as it tried to move over the slide and the amoeba wrapped itself around the Paramecium and engulfed it. It was rad! The Paramecium still struggled inside the Amoeba for a long time. The amoeba had tiny particles that flowed throughout its cytoplasm - maybe food being digested in vacuoles?? It was hard to see the nucleus because everything was so transparent. The amoeba actually "peeded" by squirting water out of its contractile vacuole! I dropped a tiny salt crystal in the water and the amoeba actually turned away and seemed to shrink. What was the salt doing to the amoeba? Will the same thing happen with other protists??
How NOT to do a Microscope Drawing! Phylum: ?
Genus: ? Observations: Couldn't see much. It was gooey-looking and looked cool. It grabbed something. No nucleus. (Too short, few or inaccurate details, no time spent in thinking about what was being seen; no analysis of what might be going on; no important connections to vocabulary or major concepts for the unit. Won't help much as a study aide.)
Pro Tips• Coverslips
• Focusing
• Adjusting the light
How do we make a slide?
You will be making 3 slides
1. Onion Cells
2. Elodea Cells
3. Cheek Cells
You will be looking at one prepared slide
1. Bacteria Cells
How to make a cheek cell slide1. Put a drop of iodine
on the slide2. Gently scrape a
toothpick over the inside of your cheek
3. Swirl it in the drop of iodine to stain the cells
4. Add a cover slip
Draw a cell and label what you can
How to make an onion cell slide1. Bend the onion
back against the curve
2. Get the thin film layer that holds the 2 sides together on your slide
3. Lay it on slide. 4. Smooth it out,
add iodine + coverslip
Draw a few cells and label what you can
How to make an elodea slide1. Cut a small piece of
elodea leaf.2. Lay it on the slide.3. Add 1 drop of water +
coverslip.4. NO STAIN
How to make a bacteria slide
1. It’s already made2. The bacteria are
purple3. They are VERY
small4. Draw several and
label what you can
You are not an expert…
• I don’t expect you to be an expert with the scopes but…
• You need to try your best• You need to show me when you think
you’ve got it.
Clean Up• Rinse and PAT dry slides and coverslips
– DO NOT WASH PREPARED SLIDES• Throw away onions & toothpicks• Put microscope on low power• Turn off microscope• Put dust cover on microscope• Return microscope to cupboard