microtubule motors text and image sources are included using the notes function of this file
Post on 21-Dec-2015
217 views
TRANSCRIPT
Microtubule
motorsQuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
Text and image sources are included
using the notes function of this
file
Intracellular
transport
vesicles
QuickTime™ and aVideo decompressor
are needed to see this picture.
VSVG-GFPand Microtubules
Fast Axonal Transport
in vivo in vitro
QuickTime™ and aVideo decompressor
are needed to see this picture.
QuickTime™ and aVideo decompressor
are needed to see this picture.
Loligo
Freeze!
QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
50 nm
microtubule
kinesin
Transportvesicle
Biophysical analysis
QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
8nm steps correspond to the spacing of tubulin dimers
Kinesin develops about 6 picoNewtons (pN) of force
In vitro motility
Fluorescent microtubules gliding over a kinesin-coated surfaceQuickTime™ and a
Sorenson Video 3 decompressorare needed to see this picture.
Kinesin
Biotech saddles the beast
QuickTime™ and aSorenson Video 3 decompressorare needed to see this picture.
A “rectifying” shape can direct traffic
QuickTime™ and aSorenson Video 3 decompressorare needed to see this picture.
Kinesins share a conserved
motor domain, placed in different
evolutionary contexts
QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
100 nm
Turning tail
Ionic strength (in vitro) Post-translational modification?
Folding modulates interactions with microtubules and cargo
400kDa Conventional Kinesin
Kinesin in vitro motility assays
Using polarity marked microtubules
Conventional kinesin is a plus-end directed motor (minus-end of track leads)
Ncd is a kinesin that side steps in a weakly minus-end directed motion to rotate the track
QuickTime™ and aYUV420 codec decompressor
are needed to see this picture.
- +seed
MAPs can block motor
activityQuickTime™ and a
TIFF (Uncompressed) decompressorare needed to see this picture.
Kinesin-driven anterograde movement of mitochondria is blocked by ectopic expression of MAPs like Tau
Cytoplasmic dynein and -COP
QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
Dynein inhibition causes outward dispersal of
lysosomes
QuickTime™ and aVideo decompressorare needed to see this picture.
Dynein family tree
QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
Spotty, perinuclear localization
Golgilocalization
Flagellardyneins
The flagellar dynein motor is (a) complex
QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
Microtubule sliding powers flagellar beating
QuickTime™ and aGIF decompressorare needed to see this picture.
QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
Dynein Mechanochemical Cycle
Axonemal abnormalities
QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
Normal Kartagener’s syndrome