New Millicell® µ-Angiogenesis Assay Kits

Studying how compounds affect angiogenesis, either to

promote or inhibit new capillary tube formation, can lead

to therapies affecting wound healing, tissue regeneration,

cardiovascular disease, stroke, tumor progression, and

more. The Millicell µ-Angiogenesis activation and inhibition

kits provide a powerful, quantitative platform for real-

time monitoring of changes in tubule formation with

unprecedented optical resolution.

Advantages•Continuous monitoring of cells

•Unique slide design eliminates any liquid

meniscus and out-of-focus areas

•Full well visualization at low magnification

•Optimized assay conditions

•Compatible with multichannel pipettes

•All-in-one slide used to grow, treat, and stain cells

•Miniaturized format reduces costs and waste by up to 80%

Optimized and easy-to-use slide-based assays with enhanced imaging capabilities

www.millipore.com/AngioSlides

Grow, treat, stain, and visualize cells all in one device

All your cells are in focus with Millicell µ-slides

Millicell µ-Slide vs. 96-Well Plate

No meniscus• Improved optics

• All cells in focus

• 10 µL inner well volume

• 15 wells

• Slide format

10x 10x

Depthof Focus

Unique well design eliminates meniscus

• Upper well contains

media/reagents

• Inner well contains

cells and/or matrix

Three simple steps, one easy µ-slide1. Addmatrixcomponents and incubate

2. Groworaddcells and incubate*

3. Addcalcein-AM and visualize

* Photos at right taken after cells were incubated 16-24 hours

Meniscus formation• Conventional optics

• Few cells in focus

• 100 µL well volume

• 96 wells

• Multiwell format

Cells w/oD, L-Sulforaphane

Cells w/D, L-Sulforaphane

Cells w/o PMA

Cells w/ PMA

Easy to quantify tube formation

µ-Slide kits promote robust tube formation

Value Pattern

0 Individual cells, well separated.

1 Cells begin to migrate and align themselves.

2 Capillary tubes visible. No sprouting.

Value Pattern

3 Sprouting of new capillary tubes visible.

4 Closed polygons begin to form.

5 Complex mesh-like structures develop.

µ-Angiogenesis Inhibition AssayThe angiogenesis inhibition kit includes ECMatrix™ to induce angiogenesis, the potent angiogenesis inhibitor

(D,L)-sulforaphane to act as a control, and calcein-AM to visualize the cells in real time.

µ-Angiogenesis Activation AssayThe angiogenesis activation kit includes fibrinogen and thrombin components to make the fibrin gel, the activation

control PMA (phorbol myristate acetate), and calcein-AM to visualize the cells in real time. (Left to right) Bright field,

calcein-AM, DAPI, and calcein-DAPI merge micrographs show robust tubule formation and lumen structure in the

presence of phorbol myristate acetate (PMA, a proangiogenic agent).

1µm 5µm 10µm 20µm 50µm

Withnostimulant LumenStructure

With50µg/mLPMAand1xITS

Description Qty/Pk Catalogue No.

NEW Millicell µ-Angiogenesis Inhibition Assay 1 MMA125

(5 slides, 75 microwells)

NEW Millicell µ-Angiogenesis Activation Assay 1 MMA130

(5 slides, 75 microwells)

Fibrin In Vitro Angiogenesis Assay (reagents only) 1 ECM630

(enough for 1 x 96 well plate)

In Vitro Angiogenesis Assay Kit (reagents only) 1 ECM625

(enough for 1 x 96 well plate)

Required Equipment/Reagents

Description Qty/Pk Catalogue No.

Accutase™ Cell Dissociation Solution 100 mL SCR005

EndoGRO™ Human umbilical vein endothelial cells (HUVEC) 1 vial (500,000 cells) SCCE001

EndoGRO LS complete medium 500 mL SCME001

EmbryoMax® ES Cell Qualified Ultra Pure Water, sterile H20 500 mL TMS-006-B

ORDERING INFORMATION

www.millipore.com

Millipore, Advancing Life Science Together, EmbryoMax, and Millicell are registered trademarks of Millipore Corporation. The M mark, ECMatrix, Accutase, ,and EndoGRO are trademarks of Millipore Corporation. Lit. No. PR2965EN00 03/10 BS-GEN-10-02933 Printed in U.S.A. © 2010 Millipore Corporation, Billerica, MA 01821 U.S.A. All rights reserved.

TO PLACE AN ORDER OR RECEIVE TECHNICAL ASSISTANCE In the U.S. and Canada, call toll-free 1 800-Millipore (1-800-645-5476)

In Europe, please call Customer Service:

France: 0825.045.645

Spain: 901.516.645 Option 1

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Italy: 848.845.645

United Kingdom: 0870.900.46.45

For other countries across Europe and the world,

please visit www.millipore.com/offices.

For Technical Service, please visit www.millipore.com/techservice.