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TRANSCRIPT
Molecular Hydrogen
Therapy
FxMED
Webinar
Monday 20th March 2017
Dr Elen ApThomas MBBS DRANZCOG
MACNEM
Who Is Dr Elen
bull Dr Elen ApThomas is a holistic medical doctor trained in both integrative and conventional medicine based in the Gold Coast Australia
bull MBBS Bachelors in Medicine and Surgery (Adelaide) Diploma Obstetrics and Gynaecology Member of Australasian College Nutritional and Environmental Medicine
bull Over 21 years of clinical experience
bull Founding doctor of a large multidisciplinary medical practice The Medical Sanctuary that has holistic doctors naturopaths osteopath chiropractor homeopath acupuncturist hypnotherapist reflexology and massage therapist
bull Weekly surgical assistance to leading gynaecological surgeon regular public speaking engagements both radio and in person peer reviewing of holistic medical text books and performs research roles for companies involved in medical therapies
bull Wife and Mother to three young girls aged 79 and 11
bull As a holistic medical doctor regularly treats patients with complex health issues that havenrsquot responded to other therapies and hence felt the need to explore obstacles to healing
Scientific Study of Miracle Water
Fiji Hunza Valley
Lourdes France
Analysis of so-called ldquoMiracle Waterrdquo from all around the world showed similar findings All of the waters had minerals and trace elements dissolved in the water However the most peculiar ingredient was MOLECULAR HYDROGEN MOLECULAR HYDROGEN typically does not exist on its own on earth but it is dissolved in these waters The discovery of Molecular Hydrogen in these waters ignited the research into Molecular Hydrogen as a therapeutic modality It has been found that the health benefits of alkaline water actually come from its molecular hydrogen effects
Nordenau Germany
Molecular Hydrogen Research Over 500 published peer review papers since 2007
Molecular Hydrogen Review
bull Med Gas Res 2015 Oct 19512 doi 101186s13618-015-0035-1 eCollection 2015
bull Beneficial biological effects and the underlying mechanisms of molecular hydrogen - comprehensive
review of 321 original articles
bull Ichihara M1 Sobue S1 Ito M2 Ito M3 Hirayama M4 Ohno K2
bull Author information
bull Abstract
bull Therapeutic effects of molecular hydrogen for a wide range of disease models and human diseases
have been investigated since 2007 A total of 321 original articles have been published from 2007 to
June 2015 Most studies have been conducted in Japan China and the USA About three-quarters of
the articles show the effects in mice and rats The number of clinical trials is increasing every year In
most diseases the effect of hydrogen has been reported with hydrogen water or hydrogen gas which
was followed by confirmation of the effect with hydrogen-rich saline Hydrogen water is mostly given ad
libitum Hydrogen gas of less than 4 is given by inhalation The effects have been reported in
essentially all organs covering 31 disease categories that can be subdivided into 166 disease models
human diseases treatment-associated pathologies and pathophysiological conditions of plants with a
predominance of oxidative stress-mediated diseases and inflammatory diseases Specific extinctions of
hydroxyl radical and peroxynitrite were initially presented but the radical-scavenging effect of
hydrogen cannot be held solely accountable for its drastic effects We and others have shown that the
effects can be mediated by modulating activities and expressions of various molecules such as Lyn ERK
p38 JNK ASK1 Akt GTP-Rac1 iNOS Nox1 NF-κB p65 IκBα STAT3 NFATc1 c-Fos and ghrelin Master
regulator(s) that drive these modifications however remain to be elucidated and are currently being
extensively investigated
Specific Conditions with
research bull Rheumatoid arthritis reduced disease progression and
oxidative stress markers reduced DAS and IL-6 levels
bull Cerebrovascular diseases reducing hypoxic ischaemia and
reperfusion injury reduced apoptosis reduced infarct ratio
bull Nephritis and nephritic syndrome
bull Diabetes and impaired glucose tolerance improved IGT and
reduced oxidative stress markers reduced LDLs and FFAs
bull Neurodegeneration Alzheimer‟s and Parkinson‟s disease
restored neural proliferation and reduced oxidative stress
bull Improved athletic performance
bull When taken with chemotherapy agents and radiotherapy has
shown a reduction in SEs (mortality loss of appetite and body
weight loss) and maintained anti-tumour activity
bull Reduced pressure ulcers and skin radiation damage
bull Interstitial cystitis
bull Mitochondrial diseases and dermatomyositis
Molecular Hydrogen Review
Articles bull KUMON K (1997) What Is Functional Water Artificial Organs 21 2-4
bull OKOUCHI S SUZUKI M SUGANO K KAGAMIMORI S amp IKEDA S (2002) Water desirable for the human body in terms of oxidation-reduction potential (ORP) to pH relationship Journal of Food Science 67 1594-1598
bull SHIRAHATA S A N E T A K A (2002) Reduced water for prevention of diseases Animal Cell Technology Basic and Applied Aspects 12 25-30
bull BALL J (2007) Recently published papers More about EGDT experimental therapies and some inconvenient truths Critical Care 11
bull WOOD K C amp GLADWIN M T (2007) The hydrogen highway to reperfusion therapy Nat Med 13 673-674
bull OHTA S (2008) [Hydrogen gas and hydrogen water act as a therapeutic and preventive antioxidant with a novel concept] Nihon Ronen Igakkai Zasshi 45 355-62
bull ZHANG S X-J J H (2008) Hydrogen-an endogenous antioxidant in the body Academic Journal of Second Military Medical University
bull 28 233-235
bull MILTON S L (2009) Hydrogen Saline a Real Gas Journal of Experimental Biology 212 v-vi
bull NAKAO A SUGIMOTO R BILLIAR T R amp MCCURRY K R (2009) Therapeutic Antioxidant Medical Gas Journal of Clinical Biochemistry and Nutrition 44 1-13
bull GEORGE J F amp AGARWAL A (2010) Hydrogen another gas with therapeutic potential Kidney International 77 85-87
bull GONCHARUK V V BAGRII V A MEL‟NIK L A CHEBOTAREVA R D amp BASHTAN S Y (2010) The use of redox potential in water treatment processes Journal of Water Chemistry and Technology 32 1-9
bull HONG Y CHEN S amp ZHANG J M (2010) Hydrogen as a selective antioxidant a review of clinical and experimental studies Journal of International Medical Research 38 1893-903
bull HUANG C S KAWAMURA T TOYODA Y amp NAKAO A (2010) Recent advances in hydrogen research as a therapeutic medical gas Free Radical Research 44 971-982
bull LIU C CUI J G SUN Q amp CAI J M (2010) Hydrogen therapy may be an effective and specific novel treatment for acute radiation syndrome Medical Hypotheses 74 145-146
bull QIAN L R LI B L CAI J M amp GAO F (2010) The Hypothesis of an Effective Safe and Novel Radioprotective Agent Hydrogen-rich Solution West Indian Medical Journal 59 122-124
bull CHUAI Y ZHAO L NI J SUN D CUI J LI B QIAN L GAO F amp CAI J (2011) A possible prevention strategy of radiation pneumonitis combine radiotherapy with aerosol inhalation of hydrogen-rich solution Medical Science Monitor 17 HY1-4
bull JUN CHEN B Z MINGCHAO LI TAO QI ZHENG CHEN XUEJUN SUN XIAO CHEN (2011) Hydrogen therapy may be a promising safe and effective treatment for diabetic erectile dysfunction a hypothesis Alternative Medicine Studies
bull OHTA S (2011) Recent progress toward hydrogen medicine potential of molecular hydrogen for preventive and therapeutic applications Curr Pharm Des 17 2241-52
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An Inaugural Symposium of the Journal Medical Gas Research Medical Gas Research 1 10
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An inaugural symposium of the journal Medical Gas Research Med Gas Res 1 10
Molecular Hydrogen
research reviews cont bull SCHOENFELD M P ANSARI R R ZAKRAJSEK J F BILLIAR T R TOYODA Y WINK D A amp NAKAO A (2011) Hydrogen therapy may reduce the risks
related to radiation-induced oxidative stress in space flight Medical Hypotheses 76 117-8
bull YONGCHUN SHEN T Y TAO WANG FUQIANG WEN (2011) Hydrogen gas a novel antioxidant for chronic obstructive pulmonary disease Journal of
Medical Colleges of PLA 26 94-97
bull GHANIZADEH A (2012) Hydrogen as a novel hypothesized emerging treatment for oxidative stress in autism European Review for Medical and Pharmacological Sciences 16 1313-4
bull GHANIZADEH A (2012) Physical exercise and intermittent administration of lactulose may improve autism symptoms through hydrogen production Medical Gas Research 2 19
bull L D amp WANG W C (2012) Can hydrogen retard the progression of osteoarthritis African Journal of Pharmacy and Pharmacology 6 352-354
bull OHNO K ITO M amp ICHIHARA M (2012) Molecular hydrogen as an emerging therapeutic medical gas for neurodegenerative and other diseases Oxidative Medicine and Cellular Longevity 2012 353152
bull OHTA S (2012) Molecular hydrogen is a novel antioxidant to efficiently reduce oxidative stress with potential for the improvement of mitochondrial diseases Biochimica et Biophysica Acta 1820 586-94
bull OSTOJIC S M (2012) Serum alkalinization and hydrogen-rich water in healthy men Mayo Clin Proc 87 501-2
bull RHEEM K E LIM Y R LEE B S LEE S K SEONG K M HYUN D H amp MIN K J (2012) Does alkaline-reduced hexagonal water delay the aging process in Drosophila Geriatr Gerontol Int 12 151-4
bull SCHOENFELD M P ANSARI R R NAKAO A amp WINK D (2012) A hypothesis on biological protection from space radiation through the use of new therapeutic gases as medical counter measures Medical Gas Research 2 8
bull SHI P amp SUN W (2012) A hypothesis on chemical mechanism of the effect of hydrogen Med Gas Res 2 17
bull SHIRAHATA S HAMASAKI T amp TERUYA K (2012) Advanced research on the health benefit of reduced water Trends in Food Science amp Technology 23 124-131
bull SHULIN LIU X S A H T (2012) Hydrogen from a biologically inert gas to a unique antioxidant
bull SIMON A R (2012) Hydrogen-supplemented drinking water just soda or an elixir of life Transplant International 25 1211-1212
bull ZHANG J Y LIU C ZHOU L QU K WANG R T TAI M H LEI J C W L WU Q F amp WANG Z X (2012) A Review of Hydrogen as a New Medical
Therapy Hepato-Gastroenterology 59 1026-1032
bull DIXON B J TANG J amp ZHANG J H (2013) The evolution of molecular hydrogen a noteworthy potential therapy with clinical significance Med Gas Res 3 10
bull GHANIZADEH A amp BERK M (2013) Molecular hydrogen an overview of its neurobiological effects and therapeutic potential for bipolar disorder and schizophrenia Med Gas Res 3 11
bull QU J amp LU X (2013) Hydrogen A promising novel treatment for hepatic encephalopathy Free Radic Biol Med
Molecular Hydrogen as a
novel antioxidant bull Akhavan O et al Hydrogen-rich water for green reduction of graphene oxide suspensions International Journal of Hydrogen Energy 2015 40(16) p 5553-
5560
bull 2Berjak P et al Cathodic amelioration of the adverse effects of oxidative stress accompanying procedures necessary for cryopreservation of embryonic
axes of recalcitrant-seeded species Seed Science Research 201121(3) p 187-203
bull 3Hanaoka K Antioxidant effects of reduced water produced by electrolysis of sodium chloride solutions Journal of Applied Electrochemistry 2001 31(12) p 1307-1313
bull 4Hanaoka K et al The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis Biophysical Chemistry 2004 107(1) p 71-82
bull 5Hiraoka A et al In Vitro Physicochemical Properties of Neutral Aqueous Solution Systems (Water Products as Drinks) Containing Hydrogen Gas 2-Carboxyethyl Germanium Sesquioxide and Platinum Nanocolloid as AdditivesJournal of Health Science 2010 56(2) p 167-174
bull 6Hiraoka A et al Studies on the properties and real existence of aqueous solution systems that are assumed to have antioxidant activities by the action of ldquoactive hydrogenrdquobdquo Journal of Health Science 2004 50(5) p 456-465
bull 7Kato S D Matsuoka and N Miwa Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR and 2 2prime-bipyridyl methods Materials Science and Engineering 2015 C 53 p 7-10
bull 8Lee MY et al Electrolyzed-reduced water protects against oxidative damage to DNA RNA and protein Appl Biochem Biotechnol 2006 135(2) p 133-44
bull 9Ohsawa I et al Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicalsNat Med 2007 13(6) p 688-694
bull 10Ohta S Molecular hydrogen as a novel antioxidant overview of the advantages of hydrogen for medical applications Methods Enzymol 2015 555 p 289-317
bull 11Park EJ et al Protective effect of electrolyzed reduced water on the paraquat-induced oxidative damage of human lymphocyte DNA Journal of the Korean Society for Applied Biological Chemistry 2005 48(2) p 155-160
bull 12Park SK et al Electrolyzed-reduced water confers increased resistance to environmental stresses Molecular amp Cellular Toxicology 2012 8(3) p 241-247
bull 13Park SK and SK Park Electrolyzed-reduced water increases resistance to oxidative stress fertility and lifespan via insulinIGF-1-like signal in C
elegans Biol Res 2013 46(2) p 147-52
bull 14Penders J R Kissner and WH Koppenol ONOOH does not react with H2 Free Radic Biol Med 2014
bull 15Qian L et al Administration of hydrogen-rich saline protects mice from lethal acute graft-versus-host disease (aGVHD) Transplantation 2013 95(5) p 658-62
bull 16Shi QH et al Hydrogen Therapy Reduces Oxidative Stress-associated Risks Following Acute and Chronic Exposure to High-altitude Environment Biomed Environ Sci 2015 28(3) p 239-41
bull 17Shirahata S et al Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage Biochemical and Biophysical Research Communications 1997 234(1) p 269-274
bull 18Yan H et al Mechanism of the lifespan extension of Caenorhabditis elegans by electrolyzed reduced waterndashparticipation of Pt nanoparticles Bioscience Biotechnology and Biochemistry 2011 75(7) p 1295-9
bull 19Yan H et al electrolyzed reduced water prolongs caenorhabditis elegans lifespan in Animal Cell Technology Basic amp Applied Aspects 2010 Springer Netherlands p 289-293
bull 20Yan HX et al Extension of the Lifespan of Caenorhabditis elegans by the Use of Electrolyzed Reduced Water Bioscience Biotechnology and Biochemistry 2010 74(10) p 2011-2015
Molecular Hydrogen
Research Human studies bull Aoki K et al Pilot study Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes Medical Gas Research
2012 2(1) p 12
bull 262Bittner AC et al Intra-Individual Ergonomics (I2E) Performance Effects of Ultra-Negative-Ion WaterProceedings of the Human Factors and Ergonomics
Society Annual Meeting SAGE Journals 2007 55(26) p 1617-1621
bull 263Drid P et al Hydrogen-Rich Water in Judo Training Psycho-Physiological Spiritual and Ethical Aspects) 2013 p 129
bull 264Fujiyama Y and T Kitahora Alkaline electrolytic water (alkali ions water) for drinking water in medicine Mizu no Tokusei to Atarashii Riyo Gijutsu Enu-Ti-Esu Tokyo 2004 p 348-457
bull 265Hiraoka A et al Effects of drinking a water product with anti-oxidant activities in vitro on the blood levels of biomarker substances for the oxidative stress Journal of Health Science 2006 52(6) p 817-820
bull 266Huang KC et al Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis Nephrology Dialysis Transplantation 2010 25(8) p 2730-2737
bull 267Huang KC et al Electrolyzed-reduced water reduced hemodialysis-induced erythrocyte impairment in end-stage renal disease patients Kidney Int 2006 70(2) p 391-8
bull 268Huang KC et al Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water Kidney Int 2003 64(2) p 704-14
bull 269Ishibashi T et al Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis an open-label pilot study Medical Gas Research 2012 2(1) p 27
bull 270Ishibashi T et al Therapeutic efficacy of infused molecular hydrogen in saline on rheumatoid arthritis A randomized double-blind placebo-controlled pilot study Int Immunopharmacol 2014 21(2) p 468-473
bull 271Ito M et al Open-label trial and randomized double-blind placebo-controlled crossover trial of hydrogen-enriched water for mitochondrial and inflammatory myopathies Medical Gas Research 2011 1(1) p 24
bull 272Kajiyama S et al Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance Nutrition Research 2008 28 p 137ndash143
bull 273Kang K-M et al Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors Medical Gas
Research 2011 1 p 11
bull 274Koyama K TY Saihara Y Ando D Goto Y Katayama A Effect of hydrogen saturated alkaline electrolyzed water on urinary oxidative stress markers after an acute exercise A randomized controlled trial Anti-aging Med 2008 4 p 117-122
bull 275Lee KJ et al Effect of electrolyzed-reduced water in vivo and in vitro examination and clinical trials in The 3rd Asia Pacific Conference on Evidence-Based Medicine 2004 Hong Kong
bull 276Li Q et al Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Med Gas Res 2013 3(1) p 20
bull 277Lu KC et al Electrolyzed reduced water attenuates hemodialysis-induced mononuclear cells apoptosis in end-stage renal disease patients Nephrology Dialysis Transplantation 2006 21 p 200-201
Molecular Hydrogen research
Human Studies cont bull Matsumoto S T Ueda and H Kakizaki Effect of supplementation with hydrogen-rich water in patients with interstitial cystitispainful bladder
syndrome Urology 2013 81(2) p 226-30
bull 279Nagatani K et al Safety of intravenous administration of hydrogen-enriched fluid in patients with acute cerebral ischemia initial clinical studies Med
Gas Res 2013 3 p 13
bull 280Nakao A et al Effectiveness of Hydrogen Rich Water on Antioxidant Status of Subjects with Potential Metabolic Syndrome-An Open Label Pilot Study Journal of Clinical Biochemistry and Nutrition 2010 46(2) p 140-149
bull 281Nakayama M et al Biological Effects of Electrolyzed Water in Hemodialysis Nephron Clinical Practice 2009112(1) p C9-C15
bull 282Nakayama M et al A novel bioactive haemodialysis system using dissolved dihydrogen (H-2) produced by water electrolysis a clinical trial Nephrology Dialysis Transplantation 2010 25(9) p 3026-3033
bull 283Ono H et al A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level Medical Gas Research 2012 2(1) p 21
bull 284Ono H et al Hydrogen(H2) treatment for acute erythymatous skin diseases A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers Medical Gas Research 2012 2(1) p 14
bull 285Ostojic SM Molecular Hydrogen in Sports Medicine New Therapeutic Perspectives Int J Sports Med 2014 (human)
bull 286Ostojic SM and MD Stojanovic Hydrogen-rich water affected blood alkalinity in physically active men Res Sports Med 2014 22(1) p 49-60
bull 287Ostojić SM et al Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women Double-blind randomized placebo-controlled cross-over trial of efficacy and safety Serbian journal of sports sciences 2011 5(1-4) p 83-89
bull 288Ostojic SM et al Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries Postgrad Med 2014 126(5) p 187-95
bull 289Shin MH et al Atomic Hydrogen Surrounded by Water Molecules H(H2O)m Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo PLoS One 2013 8(4) p e61696
bull 290Song G et al Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome Journal of Lipid Research 2013 54(7) p 1884-93
bull 291Takeuchi S et al Effects of intravenous infusion of hydrogen-rich fluid combined with intra-cisternal infusion of magnesium sulfate in severe aneurysmal subarachnoid hemorrhage study protocol for a randomized controlled trial BMC Neurol 2014 14(1) p 176
bull 292Tashiro H et al Clinical evaluation of alkali-ionized water for chronic diarrhea-placebo-controlled double blind study Digestion amp Absorption 2000 23 p 52-56
bull 293Terawaki H et al Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients a novel approach to suppress oxidative stress in the peritoneal cavity Medical Gas Research 2013 3(1) p 14
bull 294Xia C et al Effect of hydrogen-rich water on oxidative stress liver function and viral load in patients with chronic hepatitis B Clin Transl Sci 2013 6(5) p 372-5
bull 295Yang EJ et al A Clinical Trial of Orally Administered Alkaline Reduced Water 대한의생명과학회지 200713(2) p 83-89
bull 296Yeung LK et al Effect of electrolyzed reduced water hemodialysis on peripheral lymphocyte intracellular cytokine expression Nephrology Dialysis Transplantation 2006 21 p 204-204
bull 297Yoritaka A et al Pilot study of H(2) therapy in Parkinson‟s disease A randomized double-blind placebo-controlled trial Movement Disorders 2013
Molecular Hydrogen Functions
bull The most powerful selective Anti-oxidant (selective on hydroxyl radical and peroxynitrate) Does not disturb redox homeostasis ie doesn‟t reduce signalling molecules
bull Improves mitochondrial function increasing cell ATP energy production
bull Has Anti-inflammatory properties TNF ILs NF-Ks reduces cytokines etc
bull Has Anti-allergy functions stabilises mast cells bull Improved insulin sensitivity and glucose uptake
bull Cell signalling modulation via protein phosphorylation and regulates gene expression of pro-inflammatory cytokines and hormones likely via modulating peroxynitrite functions NFKB ILs TNF PGE2 FGF21 ghrelin
bull Up regulates other antioxidants including through NRF2 activity bull Passes across blood brain barrier
bull Alkalinising properties and enhancing (sparing) function of minerals
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Who Is Dr Elen
bull Dr Elen ApThomas is a holistic medical doctor trained in both integrative and conventional medicine based in the Gold Coast Australia
bull MBBS Bachelors in Medicine and Surgery (Adelaide) Diploma Obstetrics and Gynaecology Member of Australasian College Nutritional and Environmental Medicine
bull Over 21 years of clinical experience
bull Founding doctor of a large multidisciplinary medical practice The Medical Sanctuary that has holistic doctors naturopaths osteopath chiropractor homeopath acupuncturist hypnotherapist reflexology and massage therapist
bull Weekly surgical assistance to leading gynaecological surgeon regular public speaking engagements both radio and in person peer reviewing of holistic medical text books and performs research roles for companies involved in medical therapies
bull Wife and Mother to three young girls aged 79 and 11
bull As a holistic medical doctor regularly treats patients with complex health issues that havenrsquot responded to other therapies and hence felt the need to explore obstacles to healing
Scientific Study of Miracle Water
Fiji Hunza Valley
Lourdes France
Analysis of so-called ldquoMiracle Waterrdquo from all around the world showed similar findings All of the waters had minerals and trace elements dissolved in the water However the most peculiar ingredient was MOLECULAR HYDROGEN MOLECULAR HYDROGEN typically does not exist on its own on earth but it is dissolved in these waters The discovery of Molecular Hydrogen in these waters ignited the research into Molecular Hydrogen as a therapeutic modality It has been found that the health benefits of alkaline water actually come from its molecular hydrogen effects
Nordenau Germany
Molecular Hydrogen Research Over 500 published peer review papers since 2007
Molecular Hydrogen Review
bull Med Gas Res 2015 Oct 19512 doi 101186s13618-015-0035-1 eCollection 2015
bull Beneficial biological effects and the underlying mechanisms of molecular hydrogen - comprehensive
review of 321 original articles
bull Ichihara M1 Sobue S1 Ito M2 Ito M3 Hirayama M4 Ohno K2
bull Author information
bull Abstract
bull Therapeutic effects of molecular hydrogen for a wide range of disease models and human diseases
have been investigated since 2007 A total of 321 original articles have been published from 2007 to
June 2015 Most studies have been conducted in Japan China and the USA About three-quarters of
the articles show the effects in mice and rats The number of clinical trials is increasing every year In
most diseases the effect of hydrogen has been reported with hydrogen water or hydrogen gas which
was followed by confirmation of the effect with hydrogen-rich saline Hydrogen water is mostly given ad
libitum Hydrogen gas of less than 4 is given by inhalation The effects have been reported in
essentially all organs covering 31 disease categories that can be subdivided into 166 disease models
human diseases treatment-associated pathologies and pathophysiological conditions of plants with a
predominance of oxidative stress-mediated diseases and inflammatory diseases Specific extinctions of
hydroxyl radical and peroxynitrite were initially presented but the radical-scavenging effect of
hydrogen cannot be held solely accountable for its drastic effects We and others have shown that the
effects can be mediated by modulating activities and expressions of various molecules such as Lyn ERK
p38 JNK ASK1 Akt GTP-Rac1 iNOS Nox1 NF-κB p65 IκBα STAT3 NFATc1 c-Fos and ghrelin Master
regulator(s) that drive these modifications however remain to be elucidated and are currently being
extensively investigated
Specific Conditions with
research bull Rheumatoid arthritis reduced disease progression and
oxidative stress markers reduced DAS and IL-6 levels
bull Cerebrovascular diseases reducing hypoxic ischaemia and
reperfusion injury reduced apoptosis reduced infarct ratio
bull Nephritis and nephritic syndrome
bull Diabetes and impaired glucose tolerance improved IGT and
reduced oxidative stress markers reduced LDLs and FFAs
bull Neurodegeneration Alzheimer‟s and Parkinson‟s disease
restored neural proliferation and reduced oxidative stress
bull Improved athletic performance
bull When taken with chemotherapy agents and radiotherapy has
shown a reduction in SEs (mortality loss of appetite and body
weight loss) and maintained anti-tumour activity
bull Reduced pressure ulcers and skin radiation damage
bull Interstitial cystitis
bull Mitochondrial diseases and dermatomyositis
Molecular Hydrogen Review
Articles bull KUMON K (1997) What Is Functional Water Artificial Organs 21 2-4
bull OKOUCHI S SUZUKI M SUGANO K KAGAMIMORI S amp IKEDA S (2002) Water desirable for the human body in terms of oxidation-reduction potential (ORP) to pH relationship Journal of Food Science 67 1594-1598
bull SHIRAHATA S A N E T A K A (2002) Reduced water for prevention of diseases Animal Cell Technology Basic and Applied Aspects 12 25-30
bull BALL J (2007) Recently published papers More about EGDT experimental therapies and some inconvenient truths Critical Care 11
bull WOOD K C amp GLADWIN M T (2007) The hydrogen highway to reperfusion therapy Nat Med 13 673-674
bull OHTA S (2008) [Hydrogen gas and hydrogen water act as a therapeutic and preventive antioxidant with a novel concept] Nihon Ronen Igakkai Zasshi 45 355-62
bull ZHANG S X-J J H (2008) Hydrogen-an endogenous antioxidant in the body Academic Journal of Second Military Medical University
bull 28 233-235
bull MILTON S L (2009) Hydrogen Saline a Real Gas Journal of Experimental Biology 212 v-vi
bull NAKAO A SUGIMOTO R BILLIAR T R amp MCCURRY K R (2009) Therapeutic Antioxidant Medical Gas Journal of Clinical Biochemistry and Nutrition 44 1-13
bull GEORGE J F amp AGARWAL A (2010) Hydrogen another gas with therapeutic potential Kidney International 77 85-87
bull GONCHARUK V V BAGRII V A MEL‟NIK L A CHEBOTAREVA R D amp BASHTAN S Y (2010) The use of redox potential in water treatment processes Journal of Water Chemistry and Technology 32 1-9
bull HONG Y CHEN S amp ZHANG J M (2010) Hydrogen as a selective antioxidant a review of clinical and experimental studies Journal of International Medical Research 38 1893-903
bull HUANG C S KAWAMURA T TOYODA Y amp NAKAO A (2010) Recent advances in hydrogen research as a therapeutic medical gas Free Radical Research 44 971-982
bull LIU C CUI J G SUN Q amp CAI J M (2010) Hydrogen therapy may be an effective and specific novel treatment for acute radiation syndrome Medical Hypotheses 74 145-146
bull QIAN L R LI B L CAI J M amp GAO F (2010) The Hypothesis of an Effective Safe and Novel Radioprotective Agent Hydrogen-rich Solution West Indian Medical Journal 59 122-124
bull CHUAI Y ZHAO L NI J SUN D CUI J LI B QIAN L GAO F amp CAI J (2011) A possible prevention strategy of radiation pneumonitis combine radiotherapy with aerosol inhalation of hydrogen-rich solution Medical Science Monitor 17 HY1-4
bull JUN CHEN B Z MINGCHAO LI TAO QI ZHENG CHEN XUEJUN SUN XIAO CHEN (2011) Hydrogen therapy may be a promising safe and effective treatment for diabetic erectile dysfunction a hypothesis Alternative Medicine Studies
bull OHTA S (2011) Recent progress toward hydrogen medicine potential of molecular hydrogen for preventive and therapeutic applications Curr Pharm Des 17 2241-52
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An Inaugural Symposium of the Journal Medical Gas Research Medical Gas Research 1 10
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An inaugural symposium of the journal Medical Gas Research Med Gas Res 1 10
Molecular Hydrogen
research reviews cont bull SCHOENFELD M P ANSARI R R ZAKRAJSEK J F BILLIAR T R TOYODA Y WINK D A amp NAKAO A (2011) Hydrogen therapy may reduce the risks
related to radiation-induced oxidative stress in space flight Medical Hypotheses 76 117-8
bull YONGCHUN SHEN T Y TAO WANG FUQIANG WEN (2011) Hydrogen gas a novel antioxidant for chronic obstructive pulmonary disease Journal of
Medical Colleges of PLA 26 94-97
bull GHANIZADEH A (2012) Hydrogen as a novel hypothesized emerging treatment for oxidative stress in autism European Review for Medical and Pharmacological Sciences 16 1313-4
bull GHANIZADEH A (2012) Physical exercise and intermittent administration of lactulose may improve autism symptoms through hydrogen production Medical Gas Research 2 19
bull L D amp WANG W C (2012) Can hydrogen retard the progression of osteoarthritis African Journal of Pharmacy and Pharmacology 6 352-354
bull OHNO K ITO M amp ICHIHARA M (2012) Molecular hydrogen as an emerging therapeutic medical gas for neurodegenerative and other diseases Oxidative Medicine and Cellular Longevity 2012 353152
bull OHTA S (2012) Molecular hydrogen is a novel antioxidant to efficiently reduce oxidative stress with potential for the improvement of mitochondrial diseases Biochimica et Biophysica Acta 1820 586-94
bull OSTOJIC S M (2012) Serum alkalinization and hydrogen-rich water in healthy men Mayo Clin Proc 87 501-2
bull RHEEM K E LIM Y R LEE B S LEE S K SEONG K M HYUN D H amp MIN K J (2012) Does alkaline-reduced hexagonal water delay the aging process in Drosophila Geriatr Gerontol Int 12 151-4
bull SCHOENFELD M P ANSARI R R NAKAO A amp WINK D (2012) A hypothesis on biological protection from space radiation through the use of new therapeutic gases as medical counter measures Medical Gas Research 2 8
bull SHI P amp SUN W (2012) A hypothesis on chemical mechanism of the effect of hydrogen Med Gas Res 2 17
bull SHIRAHATA S HAMASAKI T amp TERUYA K (2012) Advanced research on the health benefit of reduced water Trends in Food Science amp Technology 23 124-131
bull SHULIN LIU X S A H T (2012) Hydrogen from a biologically inert gas to a unique antioxidant
bull SIMON A R (2012) Hydrogen-supplemented drinking water just soda or an elixir of life Transplant International 25 1211-1212
bull ZHANG J Y LIU C ZHOU L QU K WANG R T TAI M H LEI J C W L WU Q F amp WANG Z X (2012) A Review of Hydrogen as a New Medical
Therapy Hepato-Gastroenterology 59 1026-1032
bull DIXON B J TANG J amp ZHANG J H (2013) The evolution of molecular hydrogen a noteworthy potential therapy with clinical significance Med Gas Res 3 10
bull GHANIZADEH A amp BERK M (2013) Molecular hydrogen an overview of its neurobiological effects and therapeutic potential for bipolar disorder and schizophrenia Med Gas Res 3 11
bull QU J amp LU X (2013) Hydrogen A promising novel treatment for hepatic encephalopathy Free Radic Biol Med
Molecular Hydrogen as a
novel antioxidant bull Akhavan O et al Hydrogen-rich water for green reduction of graphene oxide suspensions International Journal of Hydrogen Energy 2015 40(16) p 5553-
5560
bull 2Berjak P et al Cathodic amelioration of the adverse effects of oxidative stress accompanying procedures necessary for cryopreservation of embryonic
axes of recalcitrant-seeded species Seed Science Research 201121(3) p 187-203
bull 3Hanaoka K Antioxidant effects of reduced water produced by electrolysis of sodium chloride solutions Journal of Applied Electrochemistry 2001 31(12) p 1307-1313
bull 4Hanaoka K et al The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis Biophysical Chemistry 2004 107(1) p 71-82
bull 5Hiraoka A et al In Vitro Physicochemical Properties of Neutral Aqueous Solution Systems (Water Products as Drinks) Containing Hydrogen Gas 2-Carboxyethyl Germanium Sesquioxide and Platinum Nanocolloid as AdditivesJournal of Health Science 2010 56(2) p 167-174
bull 6Hiraoka A et al Studies on the properties and real existence of aqueous solution systems that are assumed to have antioxidant activities by the action of ldquoactive hydrogenrdquobdquo Journal of Health Science 2004 50(5) p 456-465
bull 7Kato S D Matsuoka and N Miwa Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR and 2 2prime-bipyridyl methods Materials Science and Engineering 2015 C 53 p 7-10
bull 8Lee MY et al Electrolyzed-reduced water protects against oxidative damage to DNA RNA and protein Appl Biochem Biotechnol 2006 135(2) p 133-44
bull 9Ohsawa I et al Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicalsNat Med 2007 13(6) p 688-694
bull 10Ohta S Molecular hydrogen as a novel antioxidant overview of the advantages of hydrogen for medical applications Methods Enzymol 2015 555 p 289-317
bull 11Park EJ et al Protective effect of electrolyzed reduced water on the paraquat-induced oxidative damage of human lymphocyte DNA Journal of the Korean Society for Applied Biological Chemistry 2005 48(2) p 155-160
bull 12Park SK et al Electrolyzed-reduced water confers increased resistance to environmental stresses Molecular amp Cellular Toxicology 2012 8(3) p 241-247
bull 13Park SK and SK Park Electrolyzed-reduced water increases resistance to oxidative stress fertility and lifespan via insulinIGF-1-like signal in C
elegans Biol Res 2013 46(2) p 147-52
bull 14Penders J R Kissner and WH Koppenol ONOOH does not react with H2 Free Radic Biol Med 2014
bull 15Qian L et al Administration of hydrogen-rich saline protects mice from lethal acute graft-versus-host disease (aGVHD) Transplantation 2013 95(5) p 658-62
bull 16Shi QH et al Hydrogen Therapy Reduces Oxidative Stress-associated Risks Following Acute and Chronic Exposure to High-altitude Environment Biomed Environ Sci 2015 28(3) p 239-41
bull 17Shirahata S et al Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage Biochemical and Biophysical Research Communications 1997 234(1) p 269-274
bull 18Yan H et al Mechanism of the lifespan extension of Caenorhabditis elegans by electrolyzed reduced waterndashparticipation of Pt nanoparticles Bioscience Biotechnology and Biochemistry 2011 75(7) p 1295-9
bull 19Yan H et al electrolyzed reduced water prolongs caenorhabditis elegans lifespan in Animal Cell Technology Basic amp Applied Aspects 2010 Springer Netherlands p 289-293
bull 20Yan HX et al Extension of the Lifespan of Caenorhabditis elegans by the Use of Electrolyzed Reduced Water Bioscience Biotechnology and Biochemistry 2010 74(10) p 2011-2015
Molecular Hydrogen
Research Human studies bull Aoki K et al Pilot study Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes Medical Gas Research
2012 2(1) p 12
bull 262Bittner AC et al Intra-Individual Ergonomics (I2E) Performance Effects of Ultra-Negative-Ion WaterProceedings of the Human Factors and Ergonomics
Society Annual Meeting SAGE Journals 2007 55(26) p 1617-1621
bull 263Drid P et al Hydrogen-Rich Water in Judo Training Psycho-Physiological Spiritual and Ethical Aspects) 2013 p 129
bull 264Fujiyama Y and T Kitahora Alkaline electrolytic water (alkali ions water) for drinking water in medicine Mizu no Tokusei to Atarashii Riyo Gijutsu Enu-Ti-Esu Tokyo 2004 p 348-457
bull 265Hiraoka A et al Effects of drinking a water product with anti-oxidant activities in vitro on the blood levels of biomarker substances for the oxidative stress Journal of Health Science 2006 52(6) p 817-820
bull 266Huang KC et al Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis Nephrology Dialysis Transplantation 2010 25(8) p 2730-2737
bull 267Huang KC et al Electrolyzed-reduced water reduced hemodialysis-induced erythrocyte impairment in end-stage renal disease patients Kidney Int 2006 70(2) p 391-8
bull 268Huang KC et al Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water Kidney Int 2003 64(2) p 704-14
bull 269Ishibashi T et al Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis an open-label pilot study Medical Gas Research 2012 2(1) p 27
bull 270Ishibashi T et al Therapeutic efficacy of infused molecular hydrogen in saline on rheumatoid arthritis A randomized double-blind placebo-controlled pilot study Int Immunopharmacol 2014 21(2) p 468-473
bull 271Ito M et al Open-label trial and randomized double-blind placebo-controlled crossover trial of hydrogen-enriched water for mitochondrial and inflammatory myopathies Medical Gas Research 2011 1(1) p 24
bull 272Kajiyama S et al Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance Nutrition Research 2008 28 p 137ndash143
bull 273Kang K-M et al Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors Medical Gas
Research 2011 1 p 11
bull 274Koyama K TY Saihara Y Ando D Goto Y Katayama A Effect of hydrogen saturated alkaline electrolyzed water on urinary oxidative stress markers after an acute exercise A randomized controlled trial Anti-aging Med 2008 4 p 117-122
bull 275Lee KJ et al Effect of electrolyzed-reduced water in vivo and in vitro examination and clinical trials in The 3rd Asia Pacific Conference on Evidence-Based Medicine 2004 Hong Kong
bull 276Li Q et al Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Med Gas Res 2013 3(1) p 20
bull 277Lu KC et al Electrolyzed reduced water attenuates hemodialysis-induced mononuclear cells apoptosis in end-stage renal disease patients Nephrology Dialysis Transplantation 2006 21 p 200-201
Molecular Hydrogen research
Human Studies cont bull Matsumoto S T Ueda and H Kakizaki Effect of supplementation with hydrogen-rich water in patients with interstitial cystitispainful bladder
syndrome Urology 2013 81(2) p 226-30
bull 279Nagatani K et al Safety of intravenous administration of hydrogen-enriched fluid in patients with acute cerebral ischemia initial clinical studies Med
Gas Res 2013 3 p 13
bull 280Nakao A et al Effectiveness of Hydrogen Rich Water on Antioxidant Status of Subjects with Potential Metabolic Syndrome-An Open Label Pilot Study Journal of Clinical Biochemistry and Nutrition 2010 46(2) p 140-149
bull 281Nakayama M et al Biological Effects of Electrolyzed Water in Hemodialysis Nephron Clinical Practice 2009112(1) p C9-C15
bull 282Nakayama M et al A novel bioactive haemodialysis system using dissolved dihydrogen (H-2) produced by water electrolysis a clinical trial Nephrology Dialysis Transplantation 2010 25(9) p 3026-3033
bull 283Ono H et al A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level Medical Gas Research 2012 2(1) p 21
bull 284Ono H et al Hydrogen(H2) treatment for acute erythymatous skin diseases A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers Medical Gas Research 2012 2(1) p 14
bull 285Ostojic SM Molecular Hydrogen in Sports Medicine New Therapeutic Perspectives Int J Sports Med 2014 (human)
bull 286Ostojic SM and MD Stojanovic Hydrogen-rich water affected blood alkalinity in physically active men Res Sports Med 2014 22(1) p 49-60
bull 287Ostojić SM et al Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women Double-blind randomized placebo-controlled cross-over trial of efficacy and safety Serbian journal of sports sciences 2011 5(1-4) p 83-89
bull 288Ostojic SM et al Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries Postgrad Med 2014 126(5) p 187-95
bull 289Shin MH et al Atomic Hydrogen Surrounded by Water Molecules H(H2O)m Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo PLoS One 2013 8(4) p e61696
bull 290Song G et al Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome Journal of Lipid Research 2013 54(7) p 1884-93
bull 291Takeuchi S et al Effects of intravenous infusion of hydrogen-rich fluid combined with intra-cisternal infusion of magnesium sulfate in severe aneurysmal subarachnoid hemorrhage study protocol for a randomized controlled trial BMC Neurol 2014 14(1) p 176
bull 292Tashiro H et al Clinical evaluation of alkali-ionized water for chronic diarrhea-placebo-controlled double blind study Digestion amp Absorption 2000 23 p 52-56
bull 293Terawaki H et al Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients a novel approach to suppress oxidative stress in the peritoneal cavity Medical Gas Research 2013 3(1) p 14
bull 294Xia C et al Effect of hydrogen-rich water on oxidative stress liver function and viral load in patients with chronic hepatitis B Clin Transl Sci 2013 6(5) p 372-5
bull 295Yang EJ et al A Clinical Trial of Orally Administered Alkaline Reduced Water 대한의생명과학회지 200713(2) p 83-89
bull 296Yeung LK et al Effect of electrolyzed reduced water hemodialysis on peripheral lymphocyte intracellular cytokine expression Nephrology Dialysis Transplantation 2006 21 p 204-204
bull 297Yoritaka A et al Pilot study of H(2) therapy in Parkinson‟s disease A randomized double-blind placebo-controlled trial Movement Disorders 2013
Molecular Hydrogen Functions
bull The most powerful selective Anti-oxidant (selective on hydroxyl radical and peroxynitrate) Does not disturb redox homeostasis ie doesn‟t reduce signalling molecules
bull Improves mitochondrial function increasing cell ATP energy production
bull Has Anti-inflammatory properties TNF ILs NF-Ks reduces cytokines etc
bull Has Anti-allergy functions stabilises mast cells bull Improved insulin sensitivity and glucose uptake
bull Cell signalling modulation via protein phosphorylation and regulates gene expression of pro-inflammatory cytokines and hormones likely via modulating peroxynitrite functions NFKB ILs TNF PGE2 FGF21 ghrelin
bull Up regulates other antioxidants including through NRF2 activity bull Passes across blood brain barrier
bull Alkalinising properties and enhancing (sparing) function of minerals
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Scientific Study of Miracle Water
Fiji Hunza Valley
Lourdes France
Analysis of so-called ldquoMiracle Waterrdquo from all around the world showed similar findings All of the waters had minerals and trace elements dissolved in the water However the most peculiar ingredient was MOLECULAR HYDROGEN MOLECULAR HYDROGEN typically does not exist on its own on earth but it is dissolved in these waters The discovery of Molecular Hydrogen in these waters ignited the research into Molecular Hydrogen as a therapeutic modality It has been found that the health benefits of alkaline water actually come from its molecular hydrogen effects
Nordenau Germany
Molecular Hydrogen Research Over 500 published peer review papers since 2007
Molecular Hydrogen Review
bull Med Gas Res 2015 Oct 19512 doi 101186s13618-015-0035-1 eCollection 2015
bull Beneficial biological effects and the underlying mechanisms of molecular hydrogen - comprehensive
review of 321 original articles
bull Ichihara M1 Sobue S1 Ito M2 Ito M3 Hirayama M4 Ohno K2
bull Author information
bull Abstract
bull Therapeutic effects of molecular hydrogen for a wide range of disease models and human diseases
have been investigated since 2007 A total of 321 original articles have been published from 2007 to
June 2015 Most studies have been conducted in Japan China and the USA About three-quarters of
the articles show the effects in mice and rats The number of clinical trials is increasing every year In
most diseases the effect of hydrogen has been reported with hydrogen water or hydrogen gas which
was followed by confirmation of the effect with hydrogen-rich saline Hydrogen water is mostly given ad
libitum Hydrogen gas of less than 4 is given by inhalation The effects have been reported in
essentially all organs covering 31 disease categories that can be subdivided into 166 disease models
human diseases treatment-associated pathologies and pathophysiological conditions of plants with a
predominance of oxidative stress-mediated diseases and inflammatory diseases Specific extinctions of
hydroxyl radical and peroxynitrite were initially presented but the radical-scavenging effect of
hydrogen cannot be held solely accountable for its drastic effects We and others have shown that the
effects can be mediated by modulating activities and expressions of various molecules such as Lyn ERK
p38 JNK ASK1 Akt GTP-Rac1 iNOS Nox1 NF-κB p65 IκBα STAT3 NFATc1 c-Fos and ghrelin Master
regulator(s) that drive these modifications however remain to be elucidated and are currently being
extensively investigated
Specific Conditions with
research bull Rheumatoid arthritis reduced disease progression and
oxidative stress markers reduced DAS and IL-6 levels
bull Cerebrovascular diseases reducing hypoxic ischaemia and
reperfusion injury reduced apoptosis reduced infarct ratio
bull Nephritis and nephritic syndrome
bull Diabetes and impaired glucose tolerance improved IGT and
reduced oxidative stress markers reduced LDLs and FFAs
bull Neurodegeneration Alzheimer‟s and Parkinson‟s disease
restored neural proliferation and reduced oxidative stress
bull Improved athletic performance
bull When taken with chemotherapy agents and radiotherapy has
shown a reduction in SEs (mortality loss of appetite and body
weight loss) and maintained anti-tumour activity
bull Reduced pressure ulcers and skin radiation damage
bull Interstitial cystitis
bull Mitochondrial diseases and dermatomyositis
Molecular Hydrogen Review
Articles bull KUMON K (1997) What Is Functional Water Artificial Organs 21 2-4
bull OKOUCHI S SUZUKI M SUGANO K KAGAMIMORI S amp IKEDA S (2002) Water desirable for the human body in terms of oxidation-reduction potential (ORP) to pH relationship Journal of Food Science 67 1594-1598
bull SHIRAHATA S A N E T A K A (2002) Reduced water for prevention of diseases Animal Cell Technology Basic and Applied Aspects 12 25-30
bull BALL J (2007) Recently published papers More about EGDT experimental therapies and some inconvenient truths Critical Care 11
bull WOOD K C amp GLADWIN M T (2007) The hydrogen highway to reperfusion therapy Nat Med 13 673-674
bull OHTA S (2008) [Hydrogen gas and hydrogen water act as a therapeutic and preventive antioxidant with a novel concept] Nihon Ronen Igakkai Zasshi 45 355-62
bull ZHANG S X-J J H (2008) Hydrogen-an endogenous antioxidant in the body Academic Journal of Second Military Medical University
bull 28 233-235
bull MILTON S L (2009) Hydrogen Saline a Real Gas Journal of Experimental Biology 212 v-vi
bull NAKAO A SUGIMOTO R BILLIAR T R amp MCCURRY K R (2009) Therapeutic Antioxidant Medical Gas Journal of Clinical Biochemistry and Nutrition 44 1-13
bull GEORGE J F amp AGARWAL A (2010) Hydrogen another gas with therapeutic potential Kidney International 77 85-87
bull GONCHARUK V V BAGRII V A MEL‟NIK L A CHEBOTAREVA R D amp BASHTAN S Y (2010) The use of redox potential in water treatment processes Journal of Water Chemistry and Technology 32 1-9
bull HONG Y CHEN S amp ZHANG J M (2010) Hydrogen as a selective antioxidant a review of clinical and experimental studies Journal of International Medical Research 38 1893-903
bull HUANG C S KAWAMURA T TOYODA Y amp NAKAO A (2010) Recent advances in hydrogen research as a therapeutic medical gas Free Radical Research 44 971-982
bull LIU C CUI J G SUN Q amp CAI J M (2010) Hydrogen therapy may be an effective and specific novel treatment for acute radiation syndrome Medical Hypotheses 74 145-146
bull QIAN L R LI B L CAI J M amp GAO F (2010) The Hypothesis of an Effective Safe and Novel Radioprotective Agent Hydrogen-rich Solution West Indian Medical Journal 59 122-124
bull CHUAI Y ZHAO L NI J SUN D CUI J LI B QIAN L GAO F amp CAI J (2011) A possible prevention strategy of radiation pneumonitis combine radiotherapy with aerosol inhalation of hydrogen-rich solution Medical Science Monitor 17 HY1-4
bull JUN CHEN B Z MINGCHAO LI TAO QI ZHENG CHEN XUEJUN SUN XIAO CHEN (2011) Hydrogen therapy may be a promising safe and effective treatment for diabetic erectile dysfunction a hypothesis Alternative Medicine Studies
bull OHTA S (2011) Recent progress toward hydrogen medicine potential of molecular hydrogen for preventive and therapeutic applications Curr Pharm Des 17 2241-52
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An Inaugural Symposium of the Journal Medical Gas Research Medical Gas Research 1 10
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An inaugural symposium of the journal Medical Gas Research Med Gas Res 1 10
Molecular Hydrogen
research reviews cont bull SCHOENFELD M P ANSARI R R ZAKRAJSEK J F BILLIAR T R TOYODA Y WINK D A amp NAKAO A (2011) Hydrogen therapy may reduce the risks
related to radiation-induced oxidative stress in space flight Medical Hypotheses 76 117-8
bull YONGCHUN SHEN T Y TAO WANG FUQIANG WEN (2011) Hydrogen gas a novel antioxidant for chronic obstructive pulmonary disease Journal of
Medical Colleges of PLA 26 94-97
bull GHANIZADEH A (2012) Hydrogen as a novel hypothesized emerging treatment for oxidative stress in autism European Review for Medical and Pharmacological Sciences 16 1313-4
bull GHANIZADEH A (2012) Physical exercise and intermittent administration of lactulose may improve autism symptoms through hydrogen production Medical Gas Research 2 19
bull L D amp WANG W C (2012) Can hydrogen retard the progression of osteoarthritis African Journal of Pharmacy and Pharmacology 6 352-354
bull OHNO K ITO M amp ICHIHARA M (2012) Molecular hydrogen as an emerging therapeutic medical gas for neurodegenerative and other diseases Oxidative Medicine and Cellular Longevity 2012 353152
bull OHTA S (2012) Molecular hydrogen is a novel antioxidant to efficiently reduce oxidative stress with potential for the improvement of mitochondrial diseases Biochimica et Biophysica Acta 1820 586-94
bull OSTOJIC S M (2012) Serum alkalinization and hydrogen-rich water in healthy men Mayo Clin Proc 87 501-2
bull RHEEM K E LIM Y R LEE B S LEE S K SEONG K M HYUN D H amp MIN K J (2012) Does alkaline-reduced hexagonal water delay the aging process in Drosophila Geriatr Gerontol Int 12 151-4
bull SCHOENFELD M P ANSARI R R NAKAO A amp WINK D (2012) A hypothesis on biological protection from space radiation through the use of new therapeutic gases as medical counter measures Medical Gas Research 2 8
bull SHI P amp SUN W (2012) A hypothesis on chemical mechanism of the effect of hydrogen Med Gas Res 2 17
bull SHIRAHATA S HAMASAKI T amp TERUYA K (2012) Advanced research on the health benefit of reduced water Trends in Food Science amp Technology 23 124-131
bull SHULIN LIU X S A H T (2012) Hydrogen from a biologically inert gas to a unique antioxidant
bull SIMON A R (2012) Hydrogen-supplemented drinking water just soda or an elixir of life Transplant International 25 1211-1212
bull ZHANG J Y LIU C ZHOU L QU K WANG R T TAI M H LEI J C W L WU Q F amp WANG Z X (2012) A Review of Hydrogen as a New Medical
Therapy Hepato-Gastroenterology 59 1026-1032
bull DIXON B J TANG J amp ZHANG J H (2013) The evolution of molecular hydrogen a noteworthy potential therapy with clinical significance Med Gas Res 3 10
bull GHANIZADEH A amp BERK M (2013) Molecular hydrogen an overview of its neurobiological effects and therapeutic potential for bipolar disorder and schizophrenia Med Gas Res 3 11
bull QU J amp LU X (2013) Hydrogen A promising novel treatment for hepatic encephalopathy Free Radic Biol Med
Molecular Hydrogen as a
novel antioxidant bull Akhavan O et al Hydrogen-rich water for green reduction of graphene oxide suspensions International Journal of Hydrogen Energy 2015 40(16) p 5553-
5560
bull 2Berjak P et al Cathodic amelioration of the adverse effects of oxidative stress accompanying procedures necessary for cryopreservation of embryonic
axes of recalcitrant-seeded species Seed Science Research 201121(3) p 187-203
bull 3Hanaoka K Antioxidant effects of reduced water produced by electrolysis of sodium chloride solutions Journal of Applied Electrochemistry 2001 31(12) p 1307-1313
bull 4Hanaoka K et al The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis Biophysical Chemistry 2004 107(1) p 71-82
bull 5Hiraoka A et al In Vitro Physicochemical Properties of Neutral Aqueous Solution Systems (Water Products as Drinks) Containing Hydrogen Gas 2-Carboxyethyl Germanium Sesquioxide and Platinum Nanocolloid as AdditivesJournal of Health Science 2010 56(2) p 167-174
bull 6Hiraoka A et al Studies on the properties and real existence of aqueous solution systems that are assumed to have antioxidant activities by the action of ldquoactive hydrogenrdquobdquo Journal of Health Science 2004 50(5) p 456-465
bull 7Kato S D Matsuoka and N Miwa Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR and 2 2prime-bipyridyl methods Materials Science and Engineering 2015 C 53 p 7-10
bull 8Lee MY et al Electrolyzed-reduced water protects against oxidative damage to DNA RNA and protein Appl Biochem Biotechnol 2006 135(2) p 133-44
bull 9Ohsawa I et al Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicalsNat Med 2007 13(6) p 688-694
bull 10Ohta S Molecular hydrogen as a novel antioxidant overview of the advantages of hydrogen for medical applications Methods Enzymol 2015 555 p 289-317
bull 11Park EJ et al Protective effect of electrolyzed reduced water on the paraquat-induced oxidative damage of human lymphocyte DNA Journal of the Korean Society for Applied Biological Chemistry 2005 48(2) p 155-160
bull 12Park SK et al Electrolyzed-reduced water confers increased resistance to environmental stresses Molecular amp Cellular Toxicology 2012 8(3) p 241-247
bull 13Park SK and SK Park Electrolyzed-reduced water increases resistance to oxidative stress fertility and lifespan via insulinIGF-1-like signal in C
elegans Biol Res 2013 46(2) p 147-52
bull 14Penders J R Kissner and WH Koppenol ONOOH does not react with H2 Free Radic Biol Med 2014
bull 15Qian L et al Administration of hydrogen-rich saline protects mice from lethal acute graft-versus-host disease (aGVHD) Transplantation 2013 95(5) p 658-62
bull 16Shi QH et al Hydrogen Therapy Reduces Oxidative Stress-associated Risks Following Acute and Chronic Exposure to High-altitude Environment Biomed Environ Sci 2015 28(3) p 239-41
bull 17Shirahata S et al Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage Biochemical and Biophysical Research Communications 1997 234(1) p 269-274
bull 18Yan H et al Mechanism of the lifespan extension of Caenorhabditis elegans by electrolyzed reduced waterndashparticipation of Pt nanoparticles Bioscience Biotechnology and Biochemistry 2011 75(7) p 1295-9
bull 19Yan H et al electrolyzed reduced water prolongs caenorhabditis elegans lifespan in Animal Cell Technology Basic amp Applied Aspects 2010 Springer Netherlands p 289-293
bull 20Yan HX et al Extension of the Lifespan of Caenorhabditis elegans by the Use of Electrolyzed Reduced Water Bioscience Biotechnology and Biochemistry 2010 74(10) p 2011-2015
Molecular Hydrogen
Research Human studies bull Aoki K et al Pilot study Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes Medical Gas Research
2012 2(1) p 12
bull 262Bittner AC et al Intra-Individual Ergonomics (I2E) Performance Effects of Ultra-Negative-Ion WaterProceedings of the Human Factors and Ergonomics
Society Annual Meeting SAGE Journals 2007 55(26) p 1617-1621
bull 263Drid P et al Hydrogen-Rich Water in Judo Training Psycho-Physiological Spiritual and Ethical Aspects) 2013 p 129
bull 264Fujiyama Y and T Kitahora Alkaline electrolytic water (alkali ions water) for drinking water in medicine Mizu no Tokusei to Atarashii Riyo Gijutsu Enu-Ti-Esu Tokyo 2004 p 348-457
bull 265Hiraoka A et al Effects of drinking a water product with anti-oxidant activities in vitro on the blood levels of biomarker substances for the oxidative stress Journal of Health Science 2006 52(6) p 817-820
bull 266Huang KC et al Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis Nephrology Dialysis Transplantation 2010 25(8) p 2730-2737
bull 267Huang KC et al Electrolyzed-reduced water reduced hemodialysis-induced erythrocyte impairment in end-stage renal disease patients Kidney Int 2006 70(2) p 391-8
bull 268Huang KC et al Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water Kidney Int 2003 64(2) p 704-14
bull 269Ishibashi T et al Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis an open-label pilot study Medical Gas Research 2012 2(1) p 27
bull 270Ishibashi T et al Therapeutic efficacy of infused molecular hydrogen in saline on rheumatoid arthritis A randomized double-blind placebo-controlled pilot study Int Immunopharmacol 2014 21(2) p 468-473
bull 271Ito M et al Open-label trial and randomized double-blind placebo-controlled crossover trial of hydrogen-enriched water for mitochondrial and inflammatory myopathies Medical Gas Research 2011 1(1) p 24
bull 272Kajiyama S et al Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance Nutrition Research 2008 28 p 137ndash143
bull 273Kang K-M et al Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors Medical Gas
Research 2011 1 p 11
bull 274Koyama K TY Saihara Y Ando D Goto Y Katayama A Effect of hydrogen saturated alkaline electrolyzed water on urinary oxidative stress markers after an acute exercise A randomized controlled trial Anti-aging Med 2008 4 p 117-122
bull 275Lee KJ et al Effect of electrolyzed-reduced water in vivo and in vitro examination and clinical trials in The 3rd Asia Pacific Conference on Evidence-Based Medicine 2004 Hong Kong
bull 276Li Q et al Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Med Gas Res 2013 3(1) p 20
bull 277Lu KC et al Electrolyzed reduced water attenuates hemodialysis-induced mononuclear cells apoptosis in end-stage renal disease patients Nephrology Dialysis Transplantation 2006 21 p 200-201
Molecular Hydrogen research
Human Studies cont bull Matsumoto S T Ueda and H Kakizaki Effect of supplementation with hydrogen-rich water in patients with interstitial cystitispainful bladder
syndrome Urology 2013 81(2) p 226-30
bull 279Nagatani K et al Safety of intravenous administration of hydrogen-enriched fluid in patients with acute cerebral ischemia initial clinical studies Med
Gas Res 2013 3 p 13
bull 280Nakao A et al Effectiveness of Hydrogen Rich Water on Antioxidant Status of Subjects with Potential Metabolic Syndrome-An Open Label Pilot Study Journal of Clinical Biochemistry and Nutrition 2010 46(2) p 140-149
bull 281Nakayama M et al Biological Effects of Electrolyzed Water in Hemodialysis Nephron Clinical Practice 2009112(1) p C9-C15
bull 282Nakayama M et al A novel bioactive haemodialysis system using dissolved dihydrogen (H-2) produced by water electrolysis a clinical trial Nephrology Dialysis Transplantation 2010 25(9) p 3026-3033
bull 283Ono H et al A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level Medical Gas Research 2012 2(1) p 21
bull 284Ono H et al Hydrogen(H2) treatment for acute erythymatous skin diseases A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers Medical Gas Research 2012 2(1) p 14
bull 285Ostojic SM Molecular Hydrogen in Sports Medicine New Therapeutic Perspectives Int J Sports Med 2014 (human)
bull 286Ostojic SM and MD Stojanovic Hydrogen-rich water affected blood alkalinity in physically active men Res Sports Med 2014 22(1) p 49-60
bull 287Ostojić SM et al Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women Double-blind randomized placebo-controlled cross-over trial of efficacy and safety Serbian journal of sports sciences 2011 5(1-4) p 83-89
bull 288Ostojic SM et al Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries Postgrad Med 2014 126(5) p 187-95
bull 289Shin MH et al Atomic Hydrogen Surrounded by Water Molecules H(H2O)m Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo PLoS One 2013 8(4) p e61696
bull 290Song G et al Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome Journal of Lipid Research 2013 54(7) p 1884-93
bull 291Takeuchi S et al Effects of intravenous infusion of hydrogen-rich fluid combined with intra-cisternal infusion of magnesium sulfate in severe aneurysmal subarachnoid hemorrhage study protocol for a randomized controlled trial BMC Neurol 2014 14(1) p 176
bull 292Tashiro H et al Clinical evaluation of alkali-ionized water for chronic diarrhea-placebo-controlled double blind study Digestion amp Absorption 2000 23 p 52-56
bull 293Terawaki H et al Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients a novel approach to suppress oxidative stress in the peritoneal cavity Medical Gas Research 2013 3(1) p 14
bull 294Xia C et al Effect of hydrogen-rich water on oxidative stress liver function and viral load in patients with chronic hepatitis B Clin Transl Sci 2013 6(5) p 372-5
bull 295Yang EJ et al A Clinical Trial of Orally Administered Alkaline Reduced Water 대한의생명과학회지 200713(2) p 83-89
bull 296Yeung LK et al Effect of electrolyzed reduced water hemodialysis on peripheral lymphocyte intracellular cytokine expression Nephrology Dialysis Transplantation 2006 21 p 204-204
bull 297Yoritaka A et al Pilot study of H(2) therapy in Parkinson‟s disease A randomized double-blind placebo-controlled trial Movement Disorders 2013
Molecular Hydrogen Functions
bull The most powerful selective Anti-oxidant (selective on hydroxyl radical and peroxynitrate) Does not disturb redox homeostasis ie doesn‟t reduce signalling molecules
bull Improves mitochondrial function increasing cell ATP energy production
bull Has Anti-inflammatory properties TNF ILs NF-Ks reduces cytokines etc
bull Has Anti-allergy functions stabilises mast cells bull Improved insulin sensitivity and glucose uptake
bull Cell signalling modulation via protein phosphorylation and regulates gene expression of pro-inflammatory cytokines and hormones likely via modulating peroxynitrite functions NFKB ILs TNF PGE2 FGF21 ghrelin
bull Up regulates other antioxidants including through NRF2 activity bull Passes across blood brain barrier
bull Alkalinising properties and enhancing (sparing) function of minerals
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen Research Over 500 published peer review papers since 2007
Molecular Hydrogen Review
bull Med Gas Res 2015 Oct 19512 doi 101186s13618-015-0035-1 eCollection 2015
bull Beneficial biological effects and the underlying mechanisms of molecular hydrogen - comprehensive
review of 321 original articles
bull Ichihara M1 Sobue S1 Ito M2 Ito M3 Hirayama M4 Ohno K2
bull Author information
bull Abstract
bull Therapeutic effects of molecular hydrogen for a wide range of disease models and human diseases
have been investigated since 2007 A total of 321 original articles have been published from 2007 to
June 2015 Most studies have been conducted in Japan China and the USA About three-quarters of
the articles show the effects in mice and rats The number of clinical trials is increasing every year In
most diseases the effect of hydrogen has been reported with hydrogen water or hydrogen gas which
was followed by confirmation of the effect with hydrogen-rich saline Hydrogen water is mostly given ad
libitum Hydrogen gas of less than 4 is given by inhalation The effects have been reported in
essentially all organs covering 31 disease categories that can be subdivided into 166 disease models
human diseases treatment-associated pathologies and pathophysiological conditions of plants with a
predominance of oxidative stress-mediated diseases and inflammatory diseases Specific extinctions of
hydroxyl radical and peroxynitrite were initially presented but the radical-scavenging effect of
hydrogen cannot be held solely accountable for its drastic effects We and others have shown that the
effects can be mediated by modulating activities and expressions of various molecules such as Lyn ERK
p38 JNK ASK1 Akt GTP-Rac1 iNOS Nox1 NF-κB p65 IκBα STAT3 NFATc1 c-Fos and ghrelin Master
regulator(s) that drive these modifications however remain to be elucidated and are currently being
extensively investigated
Specific Conditions with
research bull Rheumatoid arthritis reduced disease progression and
oxidative stress markers reduced DAS and IL-6 levels
bull Cerebrovascular diseases reducing hypoxic ischaemia and
reperfusion injury reduced apoptosis reduced infarct ratio
bull Nephritis and nephritic syndrome
bull Diabetes and impaired glucose tolerance improved IGT and
reduced oxidative stress markers reduced LDLs and FFAs
bull Neurodegeneration Alzheimer‟s and Parkinson‟s disease
restored neural proliferation and reduced oxidative stress
bull Improved athletic performance
bull When taken with chemotherapy agents and radiotherapy has
shown a reduction in SEs (mortality loss of appetite and body
weight loss) and maintained anti-tumour activity
bull Reduced pressure ulcers and skin radiation damage
bull Interstitial cystitis
bull Mitochondrial diseases and dermatomyositis
Molecular Hydrogen Review
Articles bull KUMON K (1997) What Is Functional Water Artificial Organs 21 2-4
bull OKOUCHI S SUZUKI M SUGANO K KAGAMIMORI S amp IKEDA S (2002) Water desirable for the human body in terms of oxidation-reduction potential (ORP) to pH relationship Journal of Food Science 67 1594-1598
bull SHIRAHATA S A N E T A K A (2002) Reduced water for prevention of diseases Animal Cell Technology Basic and Applied Aspects 12 25-30
bull BALL J (2007) Recently published papers More about EGDT experimental therapies and some inconvenient truths Critical Care 11
bull WOOD K C amp GLADWIN M T (2007) The hydrogen highway to reperfusion therapy Nat Med 13 673-674
bull OHTA S (2008) [Hydrogen gas and hydrogen water act as a therapeutic and preventive antioxidant with a novel concept] Nihon Ronen Igakkai Zasshi 45 355-62
bull ZHANG S X-J J H (2008) Hydrogen-an endogenous antioxidant in the body Academic Journal of Second Military Medical University
bull 28 233-235
bull MILTON S L (2009) Hydrogen Saline a Real Gas Journal of Experimental Biology 212 v-vi
bull NAKAO A SUGIMOTO R BILLIAR T R amp MCCURRY K R (2009) Therapeutic Antioxidant Medical Gas Journal of Clinical Biochemistry and Nutrition 44 1-13
bull GEORGE J F amp AGARWAL A (2010) Hydrogen another gas with therapeutic potential Kidney International 77 85-87
bull GONCHARUK V V BAGRII V A MEL‟NIK L A CHEBOTAREVA R D amp BASHTAN S Y (2010) The use of redox potential in water treatment processes Journal of Water Chemistry and Technology 32 1-9
bull HONG Y CHEN S amp ZHANG J M (2010) Hydrogen as a selective antioxidant a review of clinical and experimental studies Journal of International Medical Research 38 1893-903
bull HUANG C S KAWAMURA T TOYODA Y amp NAKAO A (2010) Recent advances in hydrogen research as a therapeutic medical gas Free Radical Research 44 971-982
bull LIU C CUI J G SUN Q amp CAI J M (2010) Hydrogen therapy may be an effective and specific novel treatment for acute radiation syndrome Medical Hypotheses 74 145-146
bull QIAN L R LI B L CAI J M amp GAO F (2010) The Hypothesis of an Effective Safe and Novel Radioprotective Agent Hydrogen-rich Solution West Indian Medical Journal 59 122-124
bull CHUAI Y ZHAO L NI J SUN D CUI J LI B QIAN L GAO F amp CAI J (2011) A possible prevention strategy of radiation pneumonitis combine radiotherapy with aerosol inhalation of hydrogen-rich solution Medical Science Monitor 17 HY1-4
bull JUN CHEN B Z MINGCHAO LI TAO QI ZHENG CHEN XUEJUN SUN XIAO CHEN (2011) Hydrogen therapy may be a promising safe and effective treatment for diabetic erectile dysfunction a hypothesis Alternative Medicine Studies
bull OHTA S (2011) Recent progress toward hydrogen medicine potential of molecular hydrogen for preventive and therapeutic applications Curr Pharm Des 17 2241-52
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An Inaugural Symposium of the Journal Medical Gas Research Medical Gas Research 1 10
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An inaugural symposium of the journal Medical Gas Research Med Gas Res 1 10
Molecular Hydrogen
research reviews cont bull SCHOENFELD M P ANSARI R R ZAKRAJSEK J F BILLIAR T R TOYODA Y WINK D A amp NAKAO A (2011) Hydrogen therapy may reduce the risks
related to radiation-induced oxidative stress in space flight Medical Hypotheses 76 117-8
bull YONGCHUN SHEN T Y TAO WANG FUQIANG WEN (2011) Hydrogen gas a novel antioxidant for chronic obstructive pulmonary disease Journal of
Medical Colleges of PLA 26 94-97
bull GHANIZADEH A (2012) Hydrogen as a novel hypothesized emerging treatment for oxidative stress in autism European Review for Medical and Pharmacological Sciences 16 1313-4
bull GHANIZADEH A (2012) Physical exercise and intermittent administration of lactulose may improve autism symptoms through hydrogen production Medical Gas Research 2 19
bull L D amp WANG W C (2012) Can hydrogen retard the progression of osteoarthritis African Journal of Pharmacy and Pharmacology 6 352-354
bull OHNO K ITO M amp ICHIHARA M (2012) Molecular hydrogen as an emerging therapeutic medical gas for neurodegenerative and other diseases Oxidative Medicine and Cellular Longevity 2012 353152
bull OHTA S (2012) Molecular hydrogen is a novel antioxidant to efficiently reduce oxidative stress with potential for the improvement of mitochondrial diseases Biochimica et Biophysica Acta 1820 586-94
bull OSTOJIC S M (2012) Serum alkalinization and hydrogen-rich water in healthy men Mayo Clin Proc 87 501-2
bull RHEEM K E LIM Y R LEE B S LEE S K SEONG K M HYUN D H amp MIN K J (2012) Does alkaline-reduced hexagonal water delay the aging process in Drosophila Geriatr Gerontol Int 12 151-4
bull SCHOENFELD M P ANSARI R R NAKAO A amp WINK D (2012) A hypothesis on biological protection from space radiation through the use of new therapeutic gases as medical counter measures Medical Gas Research 2 8
bull SHI P amp SUN W (2012) A hypothesis on chemical mechanism of the effect of hydrogen Med Gas Res 2 17
bull SHIRAHATA S HAMASAKI T amp TERUYA K (2012) Advanced research on the health benefit of reduced water Trends in Food Science amp Technology 23 124-131
bull SHULIN LIU X S A H T (2012) Hydrogen from a biologically inert gas to a unique antioxidant
bull SIMON A R (2012) Hydrogen-supplemented drinking water just soda or an elixir of life Transplant International 25 1211-1212
bull ZHANG J Y LIU C ZHOU L QU K WANG R T TAI M H LEI J C W L WU Q F amp WANG Z X (2012) A Review of Hydrogen as a New Medical
Therapy Hepato-Gastroenterology 59 1026-1032
bull DIXON B J TANG J amp ZHANG J H (2013) The evolution of molecular hydrogen a noteworthy potential therapy with clinical significance Med Gas Res 3 10
bull GHANIZADEH A amp BERK M (2013) Molecular hydrogen an overview of its neurobiological effects and therapeutic potential for bipolar disorder and schizophrenia Med Gas Res 3 11
bull QU J amp LU X (2013) Hydrogen A promising novel treatment for hepatic encephalopathy Free Radic Biol Med
Molecular Hydrogen as a
novel antioxidant bull Akhavan O et al Hydrogen-rich water for green reduction of graphene oxide suspensions International Journal of Hydrogen Energy 2015 40(16) p 5553-
5560
bull 2Berjak P et al Cathodic amelioration of the adverse effects of oxidative stress accompanying procedures necessary for cryopreservation of embryonic
axes of recalcitrant-seeded species Seed Science Research 201121(3) p 187-203
bull 3Hanaoka K Antioxidant effects of reduced water produced by electrolysis of sodium chloride solutions Journal of Applied Electrochemistry 2001 31(12) p 1307-1313
bull 4Hanaoka K et al The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis Biophysical Chemistry 2004 107(1) p 71-82
bull 5Hiraoka A et al In Vitro Physicochemical Properties of Neutral Aqueous Solution Systems (Water Products as Drinks) Containing Hydrogen Gas 2-Carboxyethyl Germanium Sesquioxide and Platinum Nanocolloid as AdditivesJournal of Health Science 2010 56(2) p 167-174
bull 6Hiraoka A et al Studies on the properties and real existence of aqueous solution systems that are assumed to have antioxidant activities by the action of ldquoactive hydrogenrdquobdquo Journal of Health Science 2004 50(5) p 456-465
bull 7Kato S D Matsuoka and N Miwa Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR and 2 2prime-bipyridyl methods Materials Science and Engineering 2015 C 53 p 7-10
bull 8Lee MY et al Electrolyzed-reduced water protects against oxidative damage to DNA RNA and protein Appl Biochem Biotechnol 2006 135(2) p 133-44
bull 9Ohsawa I et al Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicalsNat Med 2007 13(6) p 688-694
bull 10Ohta S Molecular hydrogen as a novel antioxidant overview of the advantages of hydrogen for medical applications Methods Enzymol 2015 555 p 289-317
bull 11Park EJ et al Protective effect of electrolyzed reduced water on the paraquat-induced oxidative damage of human lymphocyte DNA Journal of the Korean Society for Applied Biological Chemistry 2005 48(2) p 155-160
bull 12Park SK et al Electrolyzed-reduced water confers increased resistance to environmental stresses Molecular amp Cellular Toxicology 2012 8(3) p 241-247
bull 13Park SK and SK Park Electrolyzed-reduced water increases resistance to oxidative stress fertility and lifespan via insulinIGF-1-like signal in C
elegans Biol Res 2013 46(2) p 147-52
bull 14Penders J R Kissner and WH Koppenol ONOOH does not react with H2 Free Radic Biol Med 2014
bull 15Qian L et al Administration of hydrogen-rich saline protects mice from lethal acute graft-versus-host disease (aGVHD) Transplantation 2013 95(5) p 658-62
bull 16Shi QH et al Hydrogen Therapy Reduces Oxidative Stress-associated Risks Following Acute and Chronic Exposure to High-altitude Environment Biomed Environ Sci 2015 28(3) p 239-41
bull 17Shirahata S et al Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage Biochemical and Biophysical Research Communications 1997 234(1) p 269-274
bull 18Yan H et al Mechanism of the lifespan extension of Caenorhabditis elegans by electrolyzed reduced waterndashparticipation of Pt nanoparticles Bioscience Biotechnology and Biochemistry 2011 75(7) p 1295-9
bull 19Yan H et al electrolyzed reduced water prolongs caenorhabditis elegans lifespan in Animal Cell Technology Basic amp Applied Aspects 2010 Springer Netherlands p 289-293
bull 20Yan HX et al Extension of the Lifespan of Caenorhabditis elegans by the Use of Electrolyzed Reduced Water Bioscience Biotechnology and Biochemistry 2010 74(10) p 2011-2015
Molecular Hydrogen
Research Human studies bull Aoki K et al Pilot study Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes Medical Gas Research
2012 2(1) p 12
bull 262Bittner AC et al Intra-Individual Ergonomics (I2E) Performance Effects of Ultra-Negative-Ion WaterProceedings of the Human Factors and Ergonomics
Society Annual Meeting SAGE Journals 2007 55(26) p 1617-1621
bull 263Drid P et al Hydrogen-Rich Water in Judo Training Psycho-Physiological Spiritual and Ethical Aspects) 2013 p 129
bull 264Fujiyama Y and T Kitahora Alkaline electrolytic water (alkali ions water) for drinking water in medicine Mizu no Tokusei to Atarashii Riyo Gijutsu Enu-Ti-Esu Tokyo 2004 p 348-457
bull 265Hiraoka A et al Effects of drinking a water product with anti-oxidant activities in vitro on the blood levels of biomarker substances for the oxidative stress Journal of Health Science 2006 52(6) p 817-820
bull 266Huang KC et al Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis Nephrology Dialysis Transplantation 2010 25(8) p 2730-2737
bull 267Huang KC et al Electrolyzed-reduced water reduced hemodialysis-induced erythrocyte impairment in end-stage renal disease patients Kidney Int 2006 70(2) p 391-8
bull 268Huang KC et al Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water Kidney Int 2003 64(2) p 704-14
bull 269Ishibashi T et al Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis an open-label pilot study Medical Gas Research 2012 2(1) p 27
bull 270Ishibashi T et al Therapeutic efficacy of infused molecular hydrogen in saline on rheumatoid arthritis A randomized double-blind placebo-controlled pilot study Int Immunopharmacol 2014 21(2) p 468-473
bull 271Ito M et al Open-label trial and randomized double-blind placebo-controlled crossover trial of hydrogen-enriched water for mitochondrial and inflammatory myopathies Medical Gas Research 2011 1(1) p 24
bull 272Kajiyama S et al Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance Nutrition Research 2008 28 p 137ndash143
bull 273Kang K-M et al Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors Medical Gas
Research 2011 1 p 11
bull 274Koyama K TY Saihara Y Ando D Goto Y Katayama A Effect of hydrogen saturated alkaline electrolyzed water on urinary oxidative stress markers after an acute exercise A randomized controlled trial Anti-aging Med 2008 4 p 117-122
bull 275Lee KJ et al Effect of electrolyzed-reduced water in vivo and in vitro examination and clinical trials in The 3rd Asia Pacific Conference on Evidence-Based Medicine 2004 Hong Kong
bull 276Li Q et al Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Med Gas Res 2013 3(1) p 20
bull 277Lu KC et al Electrolyzed reduced water attenuates hemodialysis-induced mononuclear cells apoptosis in end-stage renal disease patients Nephrology Dialysis Transplantation 2006 21 p 200-201
Molecular Hydrogen research
Human Studies cont bull Matsumoto S T Ueda and H Kakizaki Effect of supplementation with hydrogen-rich water in patients with interstitial cystitispainful bladder
syndrome Urology 2013 81(2) p 226-30
bull 279Nagatani K et al Safety of intravenous administration of hydrogen-enriched fluid in patients with acute cerebral ischemia initial clinical studies Med
Gas Res 2013 3 p 13
bull 280Nakao A et al Effectiveness of Hydrogen Rich Water on Antioxidant Status of Subjects with Potential Metabolic Syndrome-An Open Label Pilot Study Journal of Clinical Biochemistry and Nutrition 2010 46(2) p 140-149
bull 281Nakayama M et al Biological Effects of Electrolyzed Water in Hemodialysis Nephron Clinical Practice 2009112(1) p C9-C15
bull 282Nakayama M et al A novel bioactive haemodialysis system using dissolved dihydrogen (H-2) produced by water electrolysis a clinical trial Nephrology Dialysis Transplantation 2010 25(9) p 3026-3033
bull 283Ono H et al A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level Medical Gas Research 2012 2(1) p 21
bull 284Ono H et al Hydrogen(H2) treatment for acute erythymatous skin diseases A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers Medical Gas Research 2012 2(1) p 14
bull 285Ostojic SM Molecular Hydrogen in Sports Medicine New Therapeutic Perspectives Int J Sports Med 2014 (human)
bull 286Ostojic SM and MD Stojanovic Hydrogen-rich water affected blood alkalinity in physically active men Res Sports Med 2014 22(1) p 49-60
bull 287Ostojić SM et al Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women Double-blind randomized placebo-controlled cross-over trial of efficacy and safety Serbian journal of sports sciences 2011 5(1-4) p 83-89
bull 288Ostojic SM et al Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries Postgrad Med 2014 126(5) p 187-95
bull 289Shin MH et al Atomic Hydrogen Surrounded by Water Molecules H(H2O)m Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo PLoS One 2013 8(4) p e61696
bull 290Song G et al Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome Journal of Lipid Research 2013 54(7) p 1884-93
bull 291Takeuchi S et al Effects of intravenous infusion of hydrogen-rich fluid combined with intra-cisternal infusion of magnesium sulfate in severe aneurysmal subarachnoid hemorrhage study protocol for a randomized controlled trial BMC Neurol 2014 14(1) p 176
bull 292Tashiro H et al Clinical evaluation of alkali-ionized water for chronic diarrhea-placebo-controlled double blind study Digestion amp Absorption 2000 23 p 52-56
bull 293Terawaki H et al Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients a novel approach to suppress oxidative stress in the peritoneal cavity Medical Gas Research 2013 3(1) p 14
bull 294Xia C et al Effect of hydrogen-rich water on oxidative stress liver function and viral load in patients with chronic hepatitis B Clin Transl Sci 2013 6(5) p 372-5
bull 295Yang EJ et al A Clinical Trial of Orally Administered Alkaline Reduced Water 대한의생명과학회지 200713(2) p 83-89
bull 296Yeung LK et al Effect of electrolyzed reduced water hemodialysis on peripheral lymphocyte intracellular cytokine expression Nephrology Dialysis Transplantation 2006 21 p 204-204
bull 297Yoritaka A et al Pilot study of H(2) therapy in Parkinson‟s disease A randomized double-blind placebo-controlled trial Movement Disorders 2013
Molecular Hydrogen Functions
bull The most powerful selective Anti-oxidant (selective on hydroxyl radical and peroxynitrate) Does not disturb redox homeostasis ie doesn‟t reduce signalling molecules
bull Improves mitochondrial function increasing cell ATP energy production
bull Has Anti-inflammatory properties TNF ILs NF-Ks reduces cytokines etc
bull Has Anti-allergy functions stabilises mast cells bull Improved insulin sensitivity and glucose uptake
bull Cell signalling modulation via protein phosphorylation and regulates gene expression of pro-inflammatory cytokines and hormones likely via modulating peroxynitrite functions NFKB ILs TNF PGE2 FGF21 ghrelin
bull Up regulates other antioxidants including through NRF2 activity bull Passes across blood brain barrier
bull Alkalinising properties and enhancing (sparing) function of minerals
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen Review
bull Med Gas Res 2015 Oct 19512 doi 101186s13618-015-0035-1 eCollection 2015
bull Beneficial biological effects and the underlying mechanisms of molecular hydrogen - comprehensive
review of 321 original articles
bull Ichihara M1 Sobue S1 Ito M2 Ito M3 Hirayama M4 Ohno K2
bull Author information
bull Abstract
bull Therapeutic effects of molecular hydrogen for a wide range of disease models and human diseases
have been investigated since 2007 A total of 321 original articles have been published from 2007 to
June 2015 Most studies have been conducted in Japan China and the USA About three-quarters of
the articles show the effects in mice and rats The number of clinical trials is increasing every year In
most diseases the effect of hydrogen has been reported with hydrogen water or hydrogen gas which
was followed by confirmation of the effect with hydrogen-rich saline Hydrogen water is mostly given ad
libitum Hydrogen gas of less than 4 is given by inhalation The effects have been reported in
essentially all organs covering 31 disease categories that can be subdivided into 166 disease models
human diseases treatment-associated pathologies and pathophysiological conditions of plants with a
predominance of oxidative stress-mediated diseases and inflammatory diseases Specific extinctions of
hydroxyl radical and peroxynitrite were initially presented but the radical-scavenging effect of
hydrogen cannot be held solely accountable for its drastic effects We and others have shown that the
effects can be mediated by modulating activities and expressions of various molecules such as Lyn ERK
p38 JNK ASK1 Akt GTP-Rac1 iNOS Nox1 NF-κB p65 IκBα STAT3 NFATc1 c-Fos and ghrelin Master
regulator(s) that drive these modifications however remain to be elucidated and are currently being
extensively investigated
Specific Conditions with
research bull Rheumatoid arthritis reduced disease progression and
oxidative stress markers reduced DAS and IL-6 levels
bull Cerebrovascular diseases reducing hypoxic ischaemia and
reperfusion injury reduced apoptosis reduced infarct ratio
bull Nephritis and nephritic syndrome
bull Diabetes and impaired glucose tolerance improved IGT and
reduced oxidative stress markers reduced LDLs and FFAs
bull Neurodegeneration Alzheimer‟s and Parkinson‟s disease
restored neural proliferation and reduced oxidative stress
bull Improved athletic performance
bull When taken with chemotherapy agents and radiotherapy has
shown a reduction in SEs (mortality loss of appetite and body
weight loss) and maintained anti-tumour activity
bull Reduced pressure ulcers and skin radiation damage
bull Interstitial cystitis
bull Mitochondrial diseases and dermatomyositis
Molecular Hydrogen Review
Articles bull KUMON K (1997) What Is Functional Water Artificial Organs 21 2-4
bull OKOUCHI S SUZUKI M SUGANO K KAGAMIMORI S amp IKEDA S (2002) Water desirable for the human body in terms of oxidation-reduction potential (ORP) to pH relationship Journal of Food Science 67 1594-1598
bull SHIRAHATA S A N E T A K A (2002) Reduced water for prevention of diseases Animal Cell Technology Basic and Applied Aspects 12 25-30
bull BALL J (2007) Recently published papers More about EGDT experimental therapies and some inconvenient truths Critical Care 11
bull WOOD K C amp GLADWIN M T (2007) The hydrogen highway to reperfusion therapy Nat Med 13 673-674
bull OHTA S (2008) [Hydrogen gas and hydrogen water act as a therapeutic and preventive antioxidant with a novel concept] Nihon Ronen Igakkai Zasshi 45 355-62
bull ZHANG S X-J J H (2008) Hydrogen-an endogenous antioxidant in the body Academic Journal of Second Military Medical University
bull 28 233-235
bull MILTON S L (2009) Hydrogen Saline a Real Gas Journal of Experimental Biology 212 v-vi
bull NAKAO A SUGIMOTO R BILLIAR T R amp MCCURRY K R (2009) Therapeutic Antioxidant Medical Gas Journal of Clinical Biochemistry and Nutrition 44 1-13
bull GEORGE J F amp AGARWAL A (2010) Hydrogen another gas with therapeutic potential Kidney International 77 85-87
bull GONCHARUK V V BAGRII V A MEL‟NIK L A CHEBOTAREVA R D amp BASHTAN S Y (2010) The use of redox potential in water treatment processes Journal of Water Chemistry and Technology 32 1-9
bull HONG Y CHEN S amp ZHANG J M (2010) Hydrogen as a selective antioxidant a review of clinical and experimental studies Journal of International Medical Research 38 1893-903
bull HUANG C S KAWAMURA T TOYODA Y amp NAKAO A (2010) Recent advances in hydrogen research as a therapeutic medical gas Free Radical Research 44 971-982
bull LIU C CUI J G SUN Q amp CAI J M (2010) Hydrogen therapy may be an effective and specific novel treatment for acute radiation syndrome Medical Hypotheses 74 145-146
bull QIAN L R LI B L CAI J M amp GAO F (2010) The Hypothesis of an Effective Safe and Novel Radioprotective Agent Hydrogen-rich Solution West Indian Medical Journal 59 122-124
bull CHUAI Y ZHAO L NI J SUN D CUI J LI B QIAN L GAO F amp CAI J (2011) A possible prevention strategy of radiation pneumonitis combine radiotherapy with aerosol inhalation of hydrogen-rich solution Medical Science Monitor 17 HY1-4
bull JUN CHEN B Z MINGCHAO LI TAO QI ZHENG CHEN XUEJUN SUN XIAO CHEN (2011) Hydrogen therapy may be a promising safe and effective treatment for diabetic erectile dysfunction a hypothesis Alternative Medicine Studies
bull OHTA S (2011) Recent progress toward hydrogen medicine potential of molecular hydrogen for preventive and therapeutic applications Curr Pharm Des 17 2241-52
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An Inaugural Symposium of the Journal Medical Gas Research Medical Gas Research 1 10
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An inaugural symposium of the journal Medical Gas Research Med Gas Res 1 10
Molecular Hydrogen
research reviews cont bull SCHOENFELD M P ANSARI R R ZAKRAJSEK J F BILLIAR T R TOYODA Y WINK D A amp NAKAO A (2011) Hydrogen therapy may reduce the risks
related to radiation-induced oxidative stress in space flight Medical Hypotheses 76 117-8
bull YONGCHUN SHEN T Y TAO WANG FUQIANG WEN (2011) Hydrogen gas a novel antioxidant for chronic obstructive pulmonary disease Journal of
Medical Colleges of PLA 26 94-97
bull GHANIZADEH A (2012) Hydrogen as a novel hypothesized emerging treatment for oxidative stress in autism European Review for Medical and Pharmacological Sciences 16 1313-4
bull GHANIZADEH A (2012) Physical exercise and intermittent administration of lactulose may improve autism symptoms through hydrogen production Medical Gas Research 2 19
bull L D amp WANG W C (2012) Can hydrogen retard the progression of osteoarthritis African Journal of Pharmacy and Pharmacology 6 352-354
bull OHNO K ITO M amp ICHIHARA M (2012) Molecular hydrogen as an emerging therapeutic medical gas for neurodegenerative and other diseases Oxidative Medicine and Cellular Longevity 2012 353152
bull OHTA S (2012) Molecular hydrogen is a novel antioxidant to efficiently reduce oxidative stress with potential for the improvement of mitochondrial diseases Biochimica et Biophysica Acta 1820 586-94
bull OSTOJIC S M (2012) Serum alkalinization and hydrogen-rich water in healthy men Mayo Clin Proc 87 501-2
bull RHEEM K E LIM Y R LEE B S LEE S K SEONG K M HYUN D H amp MIN K J (2012) Does alkaline-reduced hexagonal water delay the aging process in Drosophila Geriatr Gerontol Int 12 151-4
bull SCHOENFELD M P ANSARI R R NAKAO A amp WINK D (2012) A hypothesis on biological protection from space radiation through the use of new therapeutic gases as medical counter measures Medical Gas Research 2 8
bull SHI P amp SUN W (2012) A hypothesis on chemical mechanism of the effect of hydrogen Med Gas Res 2 17
bull SHIRAHATA S HAMASAKI T amp TERUYA K (2012) Advanced research on the health benefit of reduced water Trends in Food Science amp Technology 23 124-131
bull SHULIN LIU X S A H T (2012) Hydrogen from a biologically inert gas to a unique antioxidant
bull SIMON A R (2012) Hydrogen-supplemented drinking water just soda or an elixir of life Transplant International 25 1211-1212
bull ZHANG J Y LIU C ZHOU L QU K WANG R T TAI M H LEI J C W L WU Q F amp WANG Z X (2012) A Review of Hydrogen as a New Medical
Therapy Hepato-Gastroenterology 59 1026-1032
bull DIXON B J TANG J amp ZHANG J H (2013) The evolution of molecular hydrogen a noteworthy potential therapy with clinical significance Med Gas Res 3 10
bull GHANIZADEH A amp BERK M (2013) Molecular hydrogen an overview of its neurobiological effects and therapeutic potential for bipolar disorder and schizophrenia Med Gas Res 3 11
bull QU J amp LU X (2013) Hydrogen A promising novel treatment for hepatic encephalopathy Free Radic Biol Med
Molecular Hydrogen as a
novel antioxidant bull Akhavan O et al Hydrogen-rich water for green reduction of graphene oxide suspensions International Journal of Hydrogen Energy 2015 40(16) p 5553-
5560
bull 2Berjak P et al Cathodic amelioration of the adverse effects of oxidative stress accompanying procedures necessary for cryopreservation of embryonic
axes of recalcitrant-seeded species Seed Science Research 201121(3) p 187-203
bull 3Hanaoka K Antioxidant effects of reduced water produced by electrolysis of sodium chloride solutions Journal of Applied Electrochemistry 2001 31(12) p 1307-1313
bull 4Hanaoka K et al The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis Biophysical Chemistry 2004 107(1) p 71-82
bull 5Hiraoka A et al In Vitro Physicochemical Properties of Neutral Aqueous Solution Systems (Water Products as Drinks) Containing Hydrogen Gas 2-Carboxyethyl Germanium Sesquioxide and Platinum Nanocolloid as AdditivesJournal of Health Science 2010 56(2) p 167-174
bull 6Hiraoka A et al Studies on the properties and real existence of aqueous solution systems that are assumed to have antioxidant activities by the action of ldquoactive hydrogenrdquobdquo Journal of Health Science 2004 50(5) p 456-465
bull 7Kato S D Matsuoka and N Miwa Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR and 2 2prime-bipyridyl methods Materials Science and Engineering 2015 C 53 p 7-10
bull 8Lee MY et al Electrolyzed-reduced water protects against oxidative damage to DNA RNA and protein Appl Biochem Biotechnol 2006 135(2) p 133-44
bull 9Ohsawa I et al Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicalsNat Med 2007 13(6) p 688-694
bull 10Ohta S Molecular hydrogen as a novel antioxidant overview of the advantages of hydrogen for medical applications Methods Enzymol 2015 555 p 289-317
bull 11Park EJ et al Protective effect of electrolyzed reduced water on the paraquat-induced oxidative damage of human lymphocyte DNA Journal of the Korean Society for Applied Biological Chemistry 2005 48(2) p 155-160
bull 12Park SK et al Electrolyzed-reduced water confers increased resistance to environmental stresses Molecular amp Cellular Toxicology 2012 8(3) p 241-247
bull 13Park SK and SK Park Electrolyzed-reduced water increases resistance to oxidative stress fertility and lifespan via insulinIGF-1-like signal in C
elegans Biol Res 2013 46(2) p 147-52
bull 14Penders J R Kissner and WH Koppenol ONOOH does not react with H2 Free Radic Biol Med 2014
bull 15Qian L et al Administration of hydrogen-rich saline protects mice from lethal acute graft-versus-host disease (aGVHD) Transplantation 2013 95(5) p 658-62
bull 16Shi QH et al Hydrogen Therapy Reduces Oxidative Stress-associated Risks Following Acute and Chronic Exposure to High-altitude Environment Biomed Environ Sci 2015 28(3) p 239-41
bull 17Shirahata S et al Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage Biochemical and Biophysical Research Communications 1997 234(1) p 269-274
bull 18Yan H et al Mechanism of the lifespan extension of Caenorhabditis elegans by electrolyzed reduced waterndashparticipation of Pt nanoparticles Bioscience Biotechnology and Biochemistry 2011 75(7) p 1295-9
bull 19Yan H et al electrolyzed reduced water prolongs caenorhabditis elegans lifespan in Animal Cell Technology Basic amp Applied Aspects 2010 Springer Netherlands p 289-293
bull 20Yan HX et al Extension of the Lifespan of Caenorhabditis elegans by the Use of Electrolyzed Reduced Water Bioscience Biotechnology and Biochemistry 2010 74(10) p 2011-2015
Molecular Hydrogen
Research Human studies bull Aoki K et al Pilot study Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes Medical Gas Research
2012 2(1) p 12
bull 262Bittner AC et al Intra-Individual Ergonomics (I2E) Performance Effects of Ultra-Negative-Ion WaterProceedings of the Human Factors and Ergonomics
Society Annual Meeting SAGE Journals 2007 55(26) p 1617-1621
bull 263Drid P et al Hydrogen-Rich Water in Judo Training Psycho-Physiological Spiritual and Ethical Aspects) 2013 p 129
bull 264Fujiyama Y and T Kitahora Alkaline electrolytic water (alkali ions water) for drinking water in medicine Mizu no Tokusei to Atarashii Riyo Gijutsu Enu-Ti-Esu Tokyo 2004 p 348-457
bull 265Hiraoka A et al Effects of drinking a water product with anti-oxidant activities in vitro on the blood levels of biomarker substances for the oxidative stress Journal of Health Science 2006 52(6) p 817-820
bull 266Huang KC et al Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis Nephrology Dialysis Transplantation 2010 25(8) p 2730-2737
bull 267Huang KC et al Electrolyzed-reduced water reduced hemodialysis-induced erythrocyte impairment in end-stage renal disease patients Kidney Int 2006 70(2) p 391-8
bull 268Huang KC et al Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water Kidney Int 2003 64(2) p 704-14
bull 269Ishibashi T et al Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis an open-label pilot study Medical Gas Research 2012 2(1) p 27
bull 270Ishibashi T et al Therapeutic efficacy of infused molecular hydrogen in saline on rheumatoid arthritis A randomized double-blind placebo-controlled pilot study Int Immunopharmacol 2014 21(2) p 468-473
bull 271Ito M et al Open-label trial and randomized double-blind placebo-controlled crossover trial of hydrogen-enriched water for mitochondrial and inflammatory myopathies Medical Gas Research 2011 1(1) p 24
bull 272Kajiyama S et al Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance Nutrition Research 2008 28 p 137ndash143
bull 273Kang K-M et al Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors Medical Gas
Research 2011 1 p 11
bull 274Koyama K TY Saihara Y Ando D Goto Y Katayama A Effect of hydrogen saturated alkaline electrolyzed water on urinary oxidative stress markers after an acute exercise A randomized controlled trial Anti-aging Med 2008 4 p 117-122
bull 275Lee KJ et al Effect of electrolyzed-reduced water in vivo and in vitro examination and clinical trials in The 3rd Asia Pacific Conference on Evidence-Based Medicine 2004 Hong Kong
bull 276Li Q et al Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Med Gas Res 2013 3(1) p 20
bull 277Lu KC et al Electrolyzed reduced water attenuates hemodialysis-induced mononuclear cells apoptosis in end-stage renal disease patients Nephrology Dialysis Transplantation 2006 21 p 200-201
Molecular Hydrogen research
Human Studies cont bull Matsumoto S T Ueda and H Kakizaki Effect of supplementation with hydrogen-rich water in patients with interstitial cystitispainful bladder
syndrome Urology 2013 81(2) p 226-30
bull 279Nagatani K et al Safety of intravenous administration of hydrogen-enriched fluid in patients with acute cerebral ischemia initial clinical studies Med
Gas Res 2013 3 p 13
bull 280Nakao A et al Effectiveness of Hydrogen Rich Water on Antioxidant Status of Subjects with Potential Metabolic Syndrome-An Open Label Pilot Study Journal of Clinical Biochemistry and Nutrition 2010 46(2) p 140-149
bull 281Nakayama M et al Biological Effects of Electrolyzed Water in Hemodialysis Nephron Clinical Practice 2009112(1) p C9-C15
bull 282Nakayama M et al A novel bioactive haemodialysis system using dissolved dihydrogen (H-2) produced by water electrolysis a clinical trial Nephrology Dialysis Transplantation 2010 25(9) p 3026-3033
bull 283Ono H et al A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level Medical Gas Research 2012 2(1) p 21
bull 284Ono H et al Hydrogen(H2) treatment for acute erythymatous skin diseases A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers Medical Gas Research 2012 2(1) p 14
bull 285Ostojic SM Molecular Hydrogen in Sports Medicine New Therapeutic Perspectives Int J Sports Med 2014 (human)
bull 286Ostojic SM and MD Stojanovic Hydrogen-rich water affected blood alkalinity in physically active men Res Sports Med 2014 22(1) p 49-60
bull 287Ostojić SM et al Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women Double-blind randomized placebo-controlled cross-over trial of efficacy and safety Serbian journal of sports sciences 2011 5(1-4) p 83-89
bull 288Ostojic SM et al Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries Postgrad Med 2014 126(5) p 187-95
bull 289Shin MH et al Atomic Hydrogen Surrounded by Water Molecules H(H2O)m Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo PLoS One 2013 8(4) p e61696
bull 290Song G et al Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome Journal of Lipid Research 2013 54(7) p 1884-93
bull 291Takeuchi S et al Effects of intravenous infusion of hydrogen-rich fluid combined with intra-cisternal infusion of magnesium sulfate in severe aneurysmal subarachnoid hemorrhage study protocol for a randomized controlled trial BMC Neurol 2014 14(1) p 176
bull 292Tashiro H et al Clinical evaluation of alkali-ionized water for chronic diarrhea-placebo-controlled double blind study Digestion amp Absorption 2000 23 p 52-56
bull 293Terawaki H et al Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients a novel approach to suppress oxidative stress in the peritoneal cavity Medical Gas Research 2013 3(1) p 14
bull 294Xia C et al Effect of hydrogen-rich water on oxidative stress liver function and viral load in patients with chronic hepatitis B Clin Transl Sci 2013 6(5) p 372-5
bull 295Yang EJ et al A Clinical Trial of Orally Administered Alkaline Reduced Water 대한의생명과학회지 200713(2) p 83-89
bull 296Yeung LK et al Effect of electrolyzed reduced water hemodialysis on peripheral lymphocyte intracellular cytokine expression Nephrology Dialysis Transplantation 2006 21 p 204-204
bull 297Yoritaka A et al Pilot study of H(2) therapy in Parkinson‟s disease A randomized double-blind placebo-controlled trial Movement Disorders 2013
Molecular Hydrogen Functions
bull The most powerful selective Anti-oxidant (selective on hydroxyl radical and peroxynitrate) Does not disturb redox homeostasis ie doesn‟t reduce signalling molecules
bull Improves mitochondrial function increasing cell ATP energy production
bull Has Anti-inflammatory properties TNF ILs NF-Ks reduces cytokines etc
bull Has Anti-allergy functions stabilises mast cells bull Improved insulin sensitivity and glucose uptake
bull Cell signalling modulation via protein phosphorylation and regulates gene expression of pro-inflammatory cytokines and hormones likely via modulating peroxynitrite functions NFKB ILs TNF PGE2 FGF21 ghrelin
bull Up regulates other antioxidants including through NRF2 activity bull Passes across blood brain barrier
bull Alkalinising properties and enhancing (sparing) function of minerals
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Specific Conditions with
research bull Rheumatoid arthritis reduced disease progression and
oxidative stress markers reduced DAS and IL-6 levels
bull Cerebrovascular diseases reducing hypoxic ischaemia and
reperfusion injury reduced apoptosis reduced infarct ratio
bull Nephritis and nephritic syndrome
bull Diabetes and impaired glucose tolerance improved IGT and
reduced oxidative stress markers reduced LDLs and FFAs
bull Neurodegeneration Alzheimer‟s and Parkinson‟s disease
restored neural proliferation and reduced oxidative stress
bull Improved athletic performance
bull When taken with chemotherapy agents and radiotherapy has
shown a reduction in SEs (mortality loss of appetite and body
weight loss) and maintained anti-tumour activity
bull Reduced pressure ulcers and skin radiation damage
bull Interstitial cystitis
bull Mitochondrial diseases and dermatomyositis
Molecular Hydrogen Review
Articles bull KUMON K (1997) What Is Functional Water Artificial Organs 21 2-4
bull OKOUCHI S SUZUKI M SUGANO K KAGAMIMORI S amp IKEDA S (2002) Water desirable for the human body in terms of oxidation-reduction potential (ORP) to pH relationship Journal of Food Science 67 1594-1598
bull SHIRAHATA S A N E T A K A (2002) Reduced water for prevention of diseases Animal Cell Technology Basic and Applied Aspects 12 25-30
bull BALL J (2007) Recently published papers More about EGDT experimental therapies and some inconvenient truths Critical Care 11
bull WOOD K C amp GLADWIN M T (2007) The hydrogen highway to reperfusion therapy Nat Med 13 673-674
bull OHTA S (2008) [Hydrogen gas and hydrogen water act as a therapeutic and preventive antioxidant with a novel concept] Nihon Ronen Igakkai Zasshi 45 355-62
bull ZHANG S X-J J H (2008) Hydrogen-an endogenous antioxidant in the body Academic Journal of Second Military Medical University
bull 28 233-235
bull MILTON S L (2009) Hydrogen Saline a Real Gas Journal of Experimental Biology 212 v-vi
bull NAKAO A SUGIMOTO R BILLIAR T R amp MCCURRY K R (2009) Therapeutic Antioxidant Medical Gas Journal of Clinical Biochemistry and Nutrition 44 1-13
bull GEORGE J F amp AGARWAL A (2010) Hydrogen another gas with therapeutic potential Kidney International 77 85-87
bull GONCHARUK V V BAGRII V A MEL‟NIK L A CHEBOTAREVA R D amp BASHTAN S Y (2010) The use of redox potential in water treatment processes Journal of Water Chemistry and Technology 32 1-9
bull HONG Y CHEN S amp ZHANG J M (2010) Hydrogen as a selective antioxidant a review of clinical and experimental studies Journal of International Medical Research 38 1893-903
bull HUANG C S KAWAMURA T TOYODA Y amp NAKAO A (2010) Recent advances in hydrogen research as a therapeutic medical gas Free Radical Research 44 971-982
bull LIU C CUI J G SUN Q amp CAI J M (2010) Hydrogen therapy may be an effective and specific novel treatment for acute radiation syndrome Medical Hypotheses 74 145-146
bull QIAN L R LI B L CAI J M amp GAO F (2010) The Hypothesis of an Effective Safe and Novel Radioprotective Agent Hydrogen-rich Solution West Indian Medical Journal 59 122-124
bull CHUAI Y ZHAO L NI J SUN D CUI J LI B QIAN L GAO F amp CAI J (2011) A possible prevention strategy of radiation pneumonitis combine radiotherapy with aerosol inhalation of hydrogen-rich solution Medical Science Monitor 17 HY1-4
bull JUN CHEN B Z MINGCHAO LI TAO QI ZHENG CHEN XUEJUN SUN XIAO CHEN (2011) Hydrogen therapy may be a promising safe and effective treatment for diabetic erectile dysfunction a hypothesis Alternative Medicine Studies
bull OHTA S (2011) Recent progress toward hydrogen medicine potential of molecular hydrogen for preventive and therapeutic applications Curr Pharm Des 17 2241-52
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An Inaugural Symposium of the Journal Medical Gas Research Medical Gas Research 1 10
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An inaugural symposium of the journal Medical Gas Research Med Gas Res 1 10
Molecular Hydrogen
research reviews cont bull SCHOENFELD M P ANSARI R R ZAKRAJSEK J F BILLIAR T R TOYODA Y WINK D A amp NAKAO A (2011) Hydrogen therapy may reduce the risks
related to radiation-induced oxidative stress in space flight Medical Hypotheses 76 117-8
bull YONGCHUN SHEN T Y TAO WANG FUQIANG WEN (2011) Hydrogen gas a novel antioxidant for chronic obstructive pulmonary disease Journal of
Medical Colleges of PLA 26 94-97
bull GHANIZADEH A (2012) Hydrogen as a novel hypothesized emerging treatment for oxidative stress in autism European Review for Medical and Pharmacological Sciences 16 1313-4
bull GHANIZADEH A (2012) Physical exercise and intermittent administration of lactulose may improve autism symptoms through hydrogen production Medical Gas Research 2 19
bull L D amp WANG W C (2012) Can hydrogen retard the progression of osteoarthritis African Journal of Pharmacy and Pharmacology 6 352-354
bull OHNO K ITO M amp ICHIHARA M (2012) Molecular hydrogen as an emerging therapeutic medical gas for neurodegenerative and other diseases Oxidative Medicine and Cellular Longevity 2012 353152
bull OHTA S (2012) Molecular hydrogen is a novel antioxidant to efficiently reduce oxidative stress with potential for the improvement of mitochondrial diseases Biochimica et Biophysica Acta 1820 586-94
bull OSTOJIC S M (2012) Serum alkalinization and hydrogen-rich water in healthy men Mayo Clin Proc 87 501-2
bull RHEEM K E LIM Y R LEE B S LEE S K SEONG K M HYUN D H amp MIN K J (2012) Does alkaline-reduced hexagonal water delay the aging process in Drosophila Geriatr Gerontol Int 12 151-4
bull SCHOENFELD M P ANSARI R R NAKAO A amp WINK D (2012) A hypothesis on biological protection from space radiation through the use of new therapeutic gases as medical counter measures Medical Gas Research 2 8
bull SHI P amp SUN W (2012) A hypothesis on chemical mechanism of the effect of hydrogen Med Gas Res 2 17
bull SHIRAHATA S HAMASAKI T amp TERUYA K (2012) Advanced research on the health benefit of reduced water Trends in Food Science amp Technology 23 124-131
bull SHULIN LIU X S A H T (2012) Hydrogen from a biologically inert gas to a unique antioxidant
bull SIMON A R (2012) Hydrogen-supplemented drinking water just soda or an elixir of life Transplant International 25 1211-1212
bull ZHANG J Y LIU C ZHOU L QU K WANG R T TAI M H LEI J C W L WU Q F amp WANG Z X (2012) A Review of Hydrogen as a New Medical
Therapy Hepato-Gastroenterology 59 1026-1032
bull DIXON B J TANG J amp ZHANG J H (2013) The evolution of molecular hydrogen a noteworthy potential therapy with clinical significance Med Gas Res 3 10
bull GHANIZADEH A amp BERK M (2013) Molecular hydrogen an overview of its neurobiological effects and therapeutic potential for bipolar disorder and schizophrenia Med Gas Res 3 11
bull QU J amp LU X (2013) Hydrogen A promising novel treatment for hepatic encephalopathy Free Radic Biol Med
Molecular Hydrogen as a
novel antioxidant bull Akhavan O et al Hydrogen-rich water for green reduction of graphene oxide suspensions International Journal of Hydrogen Energy 2015 40(16) p 5553-
5560
bull 2Berjak P et al Cathodic amelioration of the adverse effects of oxidative stress accompanying procedures necessary for cryopreservation of embryonic
axes of recalcitrant-seeded species Seed Science Research 201121(3) p 187-203
bull 3Hanaoka K Antioxidant effects of reduced water produced by electrolysis of sodium chloride solutions Journal of Applied Electrochemistry 2001 31(12) p 1307-1313
bull 4Hanaoka K et al The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis Biophysical Chemistry 2004 107(1) p 71-82
bull 5Hiraoka A et al In Vitro Physicochemical Properties of Neutral Aqueous Solution Systems (Water Products as Drinks) Containing Hydrogen Gas 2-Carboxyethyl Germanium Sesquioxide and Platinum Nanocolloid as AdditivesJournal of Health Science 2010 56(2) p 167-174
bull 6Hiraoka A et al Studies on the properties and real existence of aqueous solution systems that are assumed to have antioxidant activities by the action of ldquoactive hydrogenrdquobdquo Journal of Health Science 2004 50(5) p 456-465
bull 7Kato S D Matsuoka and N Miwa Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR and 2 2prime-bipyridyl methods Materials Science and Engineering 2015 C 53 p 7-10
bull 8Lee MY et al Electrolyzed-reduced water protects against oxidative damage to DNA RNA and protein Appl Biochem Biotechnol 2006 135(2) p 133-44
bull 9Ohsawa I et al Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicalsNat Med 2007 13(6) p 688-694
bull 10Ohta S Molecular hydrogen as a novel antioxidant overview of the advantages of hydrogen for medical applications Methods Enzymol 2015 555 p 289-317
bull 11Park EJ et al Protective effect of electrolyzed reduced water on the paraquat-induced oxidative damage of human lymphocyte DNA Journal of the Korean Society for Applied Biological Chemistry 2005 48(2) p 155-160
bull 12Park SK et al Electrolyzed-reduced water confers increased resistance to environmental stresses Molecular amp Cellular Toxicology 2012 8(3) p 241-247
bull 13Park SK and SK Park Electrolyzed-reduced water increases resistance to oxidative stress fertility and lifespan via insulinIGF-1-like signal in C
elegans Biol Res 2013 46(2) p 147-52
bull 14Penders J R Kissner and WH Koppenol ONOOH does not react with H2 Free Radic Biol Med 2014
bull 15Qian L et al Administration of hydrogen-rich saline protects mice from lethal acute graft-versus-host disease (aGVHD) Transplantation 2013 95(5) p 658-62
bull 16Shi QH et al Hydrogen Therapy Reduces Oxidative Stress-associated Risks Following Acute and Chronic Exposure to High-altitude Environment Biomed Environ Sci 2015 28(3) p 239-41
bull 17Shirahata S et al Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage Biochemical and Biophysical Research Communications 1997 234(1) p 269-274
bull 18Yan H et al Mechanism of the lifespan extension of Caenorhabditis elegans by electrolyzed reduced waterndashparticipation of Pt nanoparticles Bioscience Biotechnology and Biochemistry 2011 75(7) p 1295-9
bull 19Yan H et al electrolyzed reduced water prolongs caenorhabditis elegans lifespan in Animal Cell Technology Basic amp Applied Aspects 2010 Springer Netherlands p 289-293
bull 20Yan HX et al Extension of the Lifespan of Caenorhabditis elegans by the Use of Electrolyzed Reduced Water Bioscience Biotechnology and Biochemistry 2010 74(10) p 2011-2015
Molecular Hydrogen
Research Human studies bull Aoki K et al Pilot study Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes Medical Gas Research
2012 2(1) p 12
bull 262Bittner AC et al Intra-Individual Ergonomics (I2E) Performance Effects of Ultra-Negative-Ion WaterProceedings of the Human Factors and Ergonomics
Society Annual Meeting SAGE Journals 2007 55(26) p 1617-1621
bull 263Drid P et al Hydrogen-Rich Water in Judo Training Psycho-Physiological Spiritual and Ethical Aspects) 2013 p 129
bull 264Fujiyama Y and T Kitahora Alkaline electrolytic water (alkali ions water) for drinking water in medicine Mizu no Tokusei to Atarashii Riyo Gijutsu Enu-Ti-Esu Tokyo 2004 p 348-457
bull 265Hiraoka A et al Effects of drinking a water product with anti-oxidant activities in vitro on the blood levels of biomarker substances for the oxidative stress Journal of Health Science 2006 52(6) p 817-820
bull 266Huang KC et al Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis Nephrology Dialysis Transplantation 2010 25(8) p 2730-2737
bull 267Huang KC et al Electrolyzed-reduced water reduced hemodialysis-induced erythrocyte impairment in end-stage renal disease patients Kidney Int 2006 70(2) p 391-8
bull 268Huang KC et al Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water Kidney Int 2003 64(2) p 704-14
bull 269Ishibashi T et al Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis an open-label pilot study Medical Gas Research 2012 2(1) p 27
bull 270Ishibashi T et al Therapeutic efficacy of infused molecular hydrogen in saline on rheumatoid arthritis A randomized double-blind placebo-controlled pilot study Int Immunopharmacol 2014 21(2) p 468-473
bull 271Ito M et al Open-label trial and randomized double-blind placebo-controlled crossover trial of hydrogen-enriched water for mitochondrial and inflammatory myopathies Medical Gas Research 2011 1(1) p 24
bull 272Kajiyama S et al Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance Nutrition Research 2008 28 p 137ndash143
bull 273Kang K-M et al Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors Medical Gas
Research 2011 1 p 11
bull 274Koyama K TY Saihara Y Ando D Goto Y Katayama A Effect of hydrogen saturated alkaline electrolyzed water on urinary oxidative stress markers after an acute exercise A randomized controlled trial Anti-aging Med 2008 4 p 117-122
bull 275Lee KJ et al Effect of electrolyzed-reduced water in vivo and in vitro examination and clinical trials in The 3rd Asia Pacific Conference on Evidence-Based Medicine 2004 Hong Kong
bull 276Li Q et al Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Med Gas Res 2013 3(1) p 20
bull 277Lu KC et al Electrolyzed reduced water attenuates hemodialysis-induced mononuclear cells apoptosis in end-stage renal disease patients Nephrology Dialysis Transplantation 2006 21 p 200-201
Molecular Hydrogen research
Human Studies cont bull Matsumoto S T Ueda and H Kakizaki Effect of supplementation with hydrogen-rich water in patients with interstitial cystitispainful bladder
syndrome Urology 2013 81(2) p 226-30
bull 279Nagatani K et al Safety of intravenous administration of hydrogen-enriched fluid in patients with acute cerebral ischemia initial clinical studies Med
Gas Res 2013 3 p 13
bull 280Nakao A et al Effectiveness of Hydrogen Rich Water on Antioxidant Status of Subjects with Potential Metabolic Syndrome-An Open Label Pilot Study Journal of Clinical Biochemistry and Nutrition 2010 46(2) p 140-149
bull 281Nakayama M et al Biological Effects of Electrolyzed Water in Hemodialysis Nephron Clinical Practice 2009112(1) p C9-C15
bull 282Nakayama M et al A novel bioactive haemodialysis system using dissolved dihydrogen (H-2) produced by water electrolysis a clinical trial Nephrology Dialysis Transplantation 2010 25(9) p 3026-3033
bull 283Ono H et al A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level Medical Gas Research 2012 2(1) p 21
bull 284Ono H et al Hydrogen(H2) treatment for acute erythymatous skin diseases A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers Medical Gas Research 2012 2(1) p 14
bull 285Ostojic SM Molecular Hydrogen in Sports Medicine New Therapeutic Perspectives Int J Sports Med 2014 (human)
bull 286Ostojic SM and MD Stojanovic Hydrogen-rich water affected blood alkalinity in physically active men Res Sports Med 2014 22(1) p 49-60
bull 287Ostojić SM et al Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women Double-blind randomized placebo-controlled cross-over trial of efficacy and safety Serbian journal of sports sciences 2011 5(1-4) p 83-89
bull 288Ostojic SM et al Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries Postgrad Med 2014 126(5) p 187-95
bull 289Shin MH et al Atomic Hydrogen Surrounded by Water Molecules H(H2O)m Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo PLoS One 2013 8(4) p e61696
bull 290Song G et al Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome Journal of Lipid Research 2013 54(7) p 1884-93
bull 291Takeuchi S et al Effects of intravenous infusion of hydrogen-rich fluid combined with intra-cisternal infusion of magnesium sulfate in severe aneurysmal subarachnoid hemorrhage study protocol for a randomized controlled trial BMC Neurol 2014 14(1) p 176
bull 292Tashiro H et al Clinical evaluation of alkali-ionized water for chronic diarrhea-placebo-controlled double blind study Digestion amp Absorption 2000 23 p 52-56
bull 293Terawaki H et al Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients a novel approach to suppress oxidative stress in the peritoneal cavity Medical Gas Research 2013 3(1) p 14
bull 294Xia C et al Effect of hydrogen-rich water on oxidative stress liver function and viral load in patients with chronic hepatitis B Clin Transl Sci 2013 6(5) p 372-5
bull 295Yang EJ et al A Clinical Trial of Orally Administered Alkaline Reduced Water 대한의생명과학회지 200713(2) p 83-89
bull 296Yeung LK et al Effect of electrolyzed reduced water hemodialysis on peripheral lymphocyte intracellular cytokine expression Nephrology Dialysis Transplantation 2006 21 p 204-204
bull 297Yoritaka A et al Pilot study of H(2) therapy in Parkinson‟s disease A randomized double-blind placebo-controlled trial Movement Disorders 2013
Molecular Hydrogen Functions
bull The most powerful selective Anti-oxidant (selective on hydroxyl radical and peroxynitrate) Does not disturb redox homeostasis ie doesn‟t reduce signalling molecules
bull Improves mitochondrial function increasing cell ATP energy production
bull Has Anti-inflammatory properties TNF ILs NF-Ks reduces cytokines etc
bull Has Anti-allergy functions stabilises mast cells bull Improved insulin sensitivity and glucose uptake
bull Cell signalling modulation via protein phosphorylation and regulates gene expression of pro-inflammatory cytokines and hormones likely via modulating peroxynitrite functions NFKB ILs TNF PGE2 FGF21 ghrelin
bull Up regulates other antioxidants including through NRF2 activity bull Passes across blood brain barrier
bull Alkalinising properties and enhancing (sparing) function of minerals
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen Review
Articles bull KUMON K (1997) What Is Functional Water Artificial Organs 21 2-4
bull OKOUCHI S SUZUKI M SUGANO K KAGAMIMORI S amp IKEDA S (2002) Water desirable for the human body in terms of oxidation-reduction potential (ORP) to pH relationship Journal of Food Science 67 1594-1598
bull SHIRAHATA S A N E T A K A (2002) Reduced water for prevention of diseases Animal Cell Technology Basic and Applied Aspects 12 25-30
bull BALL J (2007) Recently published papers More about EGDT experimental therapies and some inconvenient truths Critical Care 11
bull WOOD K C amp GLADWIN M T (2007) The hydrogen highway to reperfusion therapy Nat Med 13 673-674
bull OHTA S (2008) [Hydrogen gas and hydrogen water act as a therapeutic and preventive antioxidant with a novel concept] Nihon Ronen Igakkai Zasshi 45 355-62
bull ZHANG S X-J J H (2008) Hydrogen-an endogenous antioxidant in the body Academic Journal of Second Military Medical University
bull 28 233-235
bull MILTON S L (2009) Hydrogen Saline a Real Gas Journal of Experimental Biology 212 v-vi
bull NAKAO A SUGIMOTO R BILLIAR T R amp MCCURRY K R (2009) Therapeutic Antioxidant Medical Gas Journal of Clinical Biochemistry and Nutrition 44 1-13
bull GEORGE J F amp AGARWAL A (2010) Hydrogen another gas with therapeutic potential Kidney International 77 85-87
bull GONCHARUK V V BAGRII V A MEL‟NIK L A CHEBOTAREVA R D amp BASHTAN S Y (2010) The use of redox potential in water treatment processes Journal of Water Chemistry and Technology 32 1-9
bull HONG Y CHEN S amp ZHANG J M (2010) Hydrogen as a selective antioxidant a review of clinical and experimental studies Journal of International Medical Research 38 1893-903
bull HUANG C S KAWAMURA T TOYODA Y amp NAKAO A (2010) Recent advances in hydrogen research as a therapeutic medical gas Free Radical Research 44 971-982
bull LIU C CUI J G SUN Q amp CAI J M (2010) Hydrogen therapy may be an effective and specific novel treatment for acute radiation syndrome Medical Hypotheses 74 145-146
bull QIAN L R LI B L CAI J M amp GAO F (2010) The Hypothesis of an Effective Safe and Novel Radioprotective Agent Hydrogen-rich Solution West Indian Medical Journal 59 122-124
bull CHUAI Y ZHAO L NI J SUN D CUI J LI B QIAN L GAO F amp CAI J (2011) A possible prevention strategy of radiation pneumonitis combine radiotherapy with aerosol inhalation of hydrogen-rich solution Medical Science Monitor 17 HY1-4
bull JUN CHEN B Z MINGCHAO LI TAO QI ZHENG CHEN XUEJUN SUN XIAO CHEN (2011) Hydrogen therapy may be a promising safe and effective treatment for diabetic erectile dysfunction a hypothesis Alternative Medicine Studies
bull OHTA S (2011) Recent progress toward hydrogen medicine potential of molecular hydrogen for preventive and therapeutic applications Curr Pharm Des 17 2241-52
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An Inaugural Symposium of the Journal Medical Gas Research Medical Gas Research 1 10
bull OHTA S NAKAO A amp OHNO K (2011) The 2011 Medical Molecular Hydrogen Symposium An inaugural symposium of the journal Medical Gas Research Med Gas Res 1 10
Molecular Hydrogen
research reviews cont bull SCHOENFELD M P ANSARI R R ZAKRAJSEK J F BILLIAR T R TOYODA Y WINK D A amp NAKAO A (2011) Hydrogen therapy may reduce the risks
related to radiation-induced oxidative stress in space flight Medical Hypotheses 76 117-8
bull YONGCHUN SHEN T Y TAO WANG FUQIANG WEN (2011) Hydrogen gas a novel antioxidant for chronic obstructive pulmonary disease Journal of
Medical Colleges of PLA 26 94-97
bull GHANIZADEH A (2012) Hydrogen as a novel hypothesized emerging treatment for oxidative stress in autism European Review for Medical and Pharmacological Sciences 16 1313-4
bull GHANIZADEH A (2012) Physical exercise and intermittent administration of lactulose may improve autism symptoms through hydrogen production Medical Gas Research 2 19
bull L D amp WANG W C (2012) Can hydrogen retard the progression of osteoarthritis African Journal of Pharmacy and Pharmacology 6 352-354
bull OHNO K ITO M amp ICHIHARA M (2012) Molecular hydrogen as an emerging therapeutic medical gas for neurodegenerative and other diseases Oxidative Medicine and Cellular Longevity 2012 353152
bull OHTA S (2012) Molecular hydrogen is a novel antioxidant to efficiently reduce oxidative stress with potential for the improvement of mitochondrial diseases Biochimica et Biophysica Acta 1820 586-94
bull OSTOJIC S M (2012) Serum alkalinization and hydrogen-rich water in healthy men Mayo Clin Proc 87 501-2
bull RHEEM K E LIM Y R LEE B S LEE S K SEONG K M HYUN D H amp MIN K J (2012) Does alkaline-reduced hexagonal water delay the aging process in Drosophila Geriatr Gerontol Int 12 151-4
bull SCHOENFELD M P ANSARI R R NAKAO A amp WINK D (2012) A hypothesis on biological protection from space radiation through the use of new therapeutic gases as medical counter measures Medical Gas Research 2 8
bull SHI P amp SUN W (2012) A hypothesis on chemical mechanism of the effect of hydrogen Med Gas Res 2 17
bull SHIRAHATA S HAMASAKI T amp TERUYA K (2012) Advanced research on the health benefit of reduced water Trends in Food Science amp Technology 23 124-131
bull SHULIN LIU X S A H T (2012) Hydrogen from a biologically inert gas to a unique antioxidant
bull SIMON A R (2012) Hydrogen-supplemented drinking water just soda or an elixir of life Transplant International 25 1211-1212
bull ZHANG J Y LIU C ZHOU L QU K WANG R T TAI M H LEI J C W L WU Q F amp WANG Z X (2012) A Review of Hydrogen as a New Medical
Therapy Hepato-Gastroenterology 59 1026-1032
bull DIXON B J TANG J amp ZHANG J H (2013) The evolution of molecular hydrogen a noteworthy potential therapy with clinical significance Med Gas Res 3 10
bull GHANIZADEH A amp BERK M (2013) Molecular hydrogen an overview of its neurobiological effects and therapeutic potential for bipolar disorder and schizophrenia Med Gas Res 3 11
bull QU J amp LU X (2013) Hydrogen A promising novel treatment for hepatic encephalopathy Free Radic Biol Med
Molecular Hydrogen as a
novel antioxidant bull Akhavan O et al Hydrogen-rich water for green reduction of graphene oxide suspensions International Journal of Hydrogen Energy 2015 40(16) p 5553-
5560
bull 2Berjak P et al Cathodic amelioration of the adverse effects of oxidative stress accompanying procedures necessary for cryopreservation of embryonic
axes of recalcitrant-seeded species Seed Science Research 201121(3) p 187-203
bull 3Hanaoka K Antioxidant effects of reduced water produced by electrolysis of sodium chloride solutions Journal of Applied Electrochemistry 2001 31(12) p 1307-1313
bull 4Hanaoka K et al The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis Biophysical Chemistry 2004 107(1) p 71-82
bull 5Hiraoka A et al In Vitro Physicochemical Properties of Neutral Aqueous Solution Systems (Water Products as Drinks) Containing Hydrogen Gas 2-Carboxyethyl Germanium Sesquioxide and Platinum Nanocolloid as AdditivesJournal of Health Science 2010 56(2) p 167-174
bull 6Hiraoka A et al Studies on the properties and real existence of aqueous solution systems that are assumed to have antioxidant activities by the action of ldquoactive hydrogenrdquobdquo Journal of Health Science 2004 50(5) p 456-465
bull 7Kato S D Matsuoka and N Miwa Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR and 2 2prime-bipyridyl methods Materials Science and Engineering 2015 C 53 p 7-10
bull 8Lee MY et al Electrolyzed-reduced water protects against oxidative damage to DNA RNA and protein Appl Biochem Biotechnol 2006 135(2) p 133-44
bull 9Ohsawa I et al Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicalsNat Med 2007 13(6) p 688-694
bull 10Ohta S Molecular hydrogen as a novel antioxidant overview of the advantages of hydrogen for medical applications Methods Enzymol 2015 555 p 289-317
bull 11Park EJ et al Protective effect of electrolyzed reduced water on the paraquat-induced oxidative damage of human lymphocyte DNA Journal of the Korean Society for Applied Biological Chemistry 2005 48(2) p 155-160
bull 12Park SK et al Electrolyzed-reduced water confers increased resistance to environmental stresses Molecular amp Cellular Toxicology 2012 8(3) p 241-247
bull 13Park SK and SK Park Electrolyzed-reduced water increases resistance to oxidative stress fertility and lifespan via insulinIGF-1-like signal in C
elegans Biol Res 2013 46(2) p 147-52
bull 14Penders J R Kissner and WH Koppenol ONOOH does not react with H2 Free Radic Biol Med 2014
bull 15Qian L et al Administration of hydrogen-rich saline protects mice from lethal acute graft-versus-host disease (aGVHD) Transplantation 2013 95(5) p 658-62
bull 16Shi QH et al Hydrogen Therapy Reduces Oxidative Stress-associated Risks Following Acute and Chronic Exposure to High-altitude Environment Biomed Environ Sci 2015 28(3) p 239-41
bull 17Shirahata S et al Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage Biochemical and Biophysical Research Communications 1997 234(1) p 269-274
bull 18Yan H et al Mechanism of the lifespan extension of Caenorhabditis elegans by electrolyzed reduced waterndashparticipation of Pt nanoparticles Bioscience Biotechnology and Biochemistry 2011 75(7) p 1295-9
bull 19Yan H et al electrolyzed reduced water prolongs caenorhabditis elegans lifespan in Animal Cell Technology Basic amp Applied Aspects 2010 Springer Netherlands p 289-293
bull 20Yan HX et al Extension of the Lifespan of Caenorhabditis elegans by the Use of Electrolyzed Reduced Water Bioscience Biotechnology and Biochemistry 2010 74(10) p 2011-2015
Molecular Hydrogen
Research Human studies bull Aoki K et al Pilot study Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes Medical Gas Research
2012 2(1) p 12
bull 262Bittner AC et al Intra-Individual Ergonomics (I2E) Performance Effects of Ultra-Negative-Ion WaterProceedings of the Human Factors and Ergonomics
Society Annual Meeting SAGE Journals 2007 55(26) p 1617-1621
bull 263Drid P et al Hydrogen-Rich Water in Judo Training Psycho-Physiological Spiritual and Ethical Aspects) 2013 p 129
bull 264Fujiyama Y and T Kitahora Alkaline electrolytic water (alkali ions water) for drinking water in medicine Mizu no Tokusei to Atarashii Riyo Gijutsu Enu-Ti-Esu Tokyo 2004 p 348-457
bull 265Hiraoka A et al Effects of drinking a water product with anti-oxidant activities in vitro on the blood levels of biomarker substances for the oxidative stress Journal of Health Science 2006 52(6) p 817-820
bull 266Huang KC et al Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis Nephrology Dialysis Transplantation 2010 25(8) p 2730-2737
bull 267Huang KC et al Electrolyzed-reduced water reduced hemodialysis-induced erythrocyte impairment in end-stage renal disease patients Kidney Int 2006 70(2) p 391-8
bull 268Huang KC et al Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water Kidney Int 2003 64(2) p 704-14
bull 269Ishibashi T et al Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis an open-label pilot study Medical Gas Research 2012 2(1) p 27
bull 270Ishibashi T et al Therapeutic efficacy of infused molecular hydrogen in saline on rheumatoid arthritis A randomized double-blind placebo-controlled pilot study Int Immunopharmacol 2014 21(2) p 468-473
bull 271Ito M et al Open-label trial and randomized double-blind placebo-controlled crossover trial of hydrogen-enriched water for mitochondrial and inflammatory myopathies Medical Gas Research 2011 1(1) p 24
bull 272Kajiyama S et al Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance Nutrition Research 2008 28 p 137ndash143
bull 273Kang K-M et al Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors Medical Gas
Research 2011 1 p 11
bull 274Koyama K TY Saihara Y Ando D Goto Y Katayama A Effect of hydrogen saturated alkaline electrolyzed water on urinary oxidative stress markers after an acute exercise A randomized controlled trial Anti-aging Med 2008 4 p 117-122
bull 275Lee KJ et al Effect of electrolyzed-reduced water in vivo and in vitro examination and clinical trials in The 3rd Asia Pacific Conference on Evidence-Based Medicine 2004 Hong Kong
bull 276Li Q et al Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Med Gas Res 2013 3(1) p 20
bull 277Lu KC et al Electrolyzed reduced water attenuates hemodialysis-induced mononuclear cells apoptosis in end-stage renal disease patients Nephrology Dialysis Transplantation 2006 21 p 200-201
Molecular Hydrogen research
Human Studies cont bull Matsumoto S T Ueda and H Kakizaki Effect of supplementation with hydrogen-rich water in patients with interstitial cystitispainful bladder
syndrome Urology 2013 81(2) p 226-30
bull 279Nagatani K et al Safety of intravenous administration of hydrogen-enriched fluid in patients with acute cerebral ischemia initial clinical studies Med
Gas Res 2013 3 p 13
bull 280Nakao A et al Effectiveness of Hydrogen Rich Water on Antioxidant Status of Subjects with Potential Metabolic Syndrome-An Open Label Pilot Study Journal of Clinical Biochemistry and Nutrition 2010 46(2) p 140-149
bull 281Nakayama M et al Biological Effects of Electrolyzed Water in Hemodialysis Nephron Clinical Practice 2009112(1) p C9-C15
bull 282Nakayama M et al A novel bioactive haemodialysis system using dissolved dihydrogen (H-2) produced by water electrolysis a clinical trial Nephrology Dialysis Transplantation 2010 25(9) p 3026-3033
bull 283Ono H et al A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level Medical Gas Research 2012 2(1) p 21
bull 284Ono H et al Hydrogen(H2) treatment for acute erythymatous skin diseases A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers Medical Gas Research 2012 2(1) p 14
bull 285Ostojic SM Molecular Hydrogen in Sports Medicine New Therapeutic Perspectives Int J Sports Med 2014 (human)
bull 286Ostojic SM and MD Stojanovic Hydrogen-rich water affected blood alkalinity in physically active men Res Sports Med 2014 22(1) p 49-60
bull 287Ostojić SM et al Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women Double-blind randomized placebo-controlled cross-over trial of efficacy and safety Serbian journal of sports sciences 2011 5(1-4) p 83-89
bull 288Ostojic SM et al Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries Postgrad Med 2014 126(5) p 187-95
bull 289Shin MH et al Atomic Hydrogen Surrounded by Water Molecules H(H2O)m Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo PLoS One 2013 8(4) p e61696
bull 290Song G et al Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome Journal of Lipid Research 2013 54(7) p 1884-93
bull 291Takeuchi S et al Effects of intravenous infusion of hydrogen-rich fluid combined with intra-cisternal infusion of magnesium sulfate in severe aneurysmal subarachnoid hemorrhage study protocol for a randomized controlled trial BMC Neurol 2014 14(1) p 176
bull 292Tashiro H et al Clinical evaluation of alkali-ionized water for chronic diarrhea-placebo-controlled double blind study Digestion amp Absorption 2000 23 p 52-56
bull 293Terawaki H et al Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients a novel approach to suppress oxidative stress in the peritoneal cavity Medical Gas Research 2013 3(1) p 14
bull 294Xia C et al Effect of hydrogen-rich water on oxidative stress liver function and viral load in patients with chronic hepatitis B Clin Transl Sci 2013 6(5) p 372-5
bull 295Yang EJ et al A Clinical Trial of Orally Administered Alkaline Reduced Water 대한의생명과학회지 200713(2) p 83-89
bull 296Yeung LK et al Effect of electrolyzed reduced water hemodialysis on peripheral lymphocyte intracellular cytokine expression Nephrology Dialysis Transplantation 2006 21 p 204-204
bull 297Yoritaka A et al Pilot study of H(2) therapy in Parkinson‟s disease A randomized double-blind placebo-controlled trial Movement Disorders 2013
Molecular Hydrogen Functions
bull The most powerful selective Anti-oxidant (selective on hydroxyl radical and peroxynitrate) Does not disturb redox homeostasis ie doesn‟t reduce signalling molecules
bull Improves mitochondrial function increasing cell ATP energy production
bull Has Anti-inflammatory properties TNF ILs NF-Ks reduces cytokines etc
bull Has Anti-allergy functions stabilises mast cells bull Improved insulin sensitivity and glucose uptake
bull Cell signalling modulation via protein phosphorylation and regulates gene expression of pro-inflammatory cytokines and hormones likely via modulating peroxynitrite functions NFKB ILs TNF PGE2 FGF21 ghrelin
bull Up regulates other antioxidants including through NRF2 activity bull Passes across blood brain barrier
bull Alkalinising properties and enhancing (sparing) function of minerals
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen
research reviews cont bull SCHOENFELD M P ANSARI R R ZAKRAJSEK J F BILLIAR T R TOYODA Y WINK D A amp NAKAO A (2011) Hydrogen therapy may reduce the risks
related to radiation-induced oxidative stress in space flight Medical Hypotheses 76 117-8
bull YONGCHUN SHEN T Y TAO WANG FUQIANG WEN (2011) Hydrogen gas a novel antioxidant for chronic obstructive pulmonary disease Journal of
Medical Colleges of PLA 26 94-97
bull GHANIZADEH A (2012) Hydrogen as a novel hypothesized emerging treatment for oxidative stress in autism European Review for Medical and Pharmacological Sciences 16 1313-4
bull GHANIZADEH A (2012) Physical exercise and intermittent administration of lactulose may improve autism symptoms through hydrogen production Medical Gas Research 2 19
bull L D amp WANG W C (2012) Can hydrogen retard the progression of osteoarthritis African Journal of Pharmacy and Pharmacology 6 352-354
bull OHNO K ITO M amp ICHIHARA M (2012) Molecular hydrogen as an emerging therapeutic medical gas for neurodegenerative and other diseases Oxidative Medicine and Cellular Longevity 2012 353152
bull OHTA S (2012) Molecular hydrogen is a novel antioxidant to efficiently reduce oxidative stress with potential for the improvement of mitochondrial diseases Biochimica et Biophysica Acta 1820 586-94
bull OSTOJIC S M (2012) Serum alkalinization and hydrogen-rich water in healthy men Mayo Clin Proc 87 501-2
bull RHEEM K E LIM Y R LEE B S LEE S K SEONG K M HYUN D H amp MIN K J (2012) Does alkaline-reduced hexagonal water delay the aging process in Drosophila Geriatr Gerontol Int 12 151-4
bull SCHOENFELD M P ANSARI R R NAKAO A amp WINK D (2012) A hypothesis on biological protection from space radiation through the use of new therapeutic gases as medical counter measures Medical Gas Research 2 8
bull SHI P amp SUN W (2012) A hypothesis on chemical mechanism of the effect of hydrogen Med Gas Res 2 17
bull SHIRAHATA S HAMASAKI T amp TERUYA K (2012) Advanced research on the health benefit of reduced water Trends in Food Science amp Technology 23 124-131
bull SHULIN LIU X S A H T (2012) Hydrogen from a biologically inert gas to a unique antioxidant
bull SIMON A R (2012) Hydrogen-supplemented drinking water just soda or an elixir of life Transplant International 25 1211-1212
bull ZHANG J Y LIU C ZHOU L QU K WANG R T TAI M H LEI J C W L WU Q F amp WANG Z X (2012) A Review of Hydrogen as a New Medical
Therapy Hepato-Gastroenterology 59 1026-1032
bull DIXON B J TANG J amp ZHANG J H (2013) The evolution of molecular hydrogen a noteworthy potential therapy with clinical significance Med Gas Res 3 10
bull GHANIZADEH A amp BERK M (2013) Molecular hydrogen an overview of its neurobiological effects and therapeutic potential for bipolar disorder and schizophrenia Med Gas Res 3 11
bull QU J amp LU X (2013) Hydrogen A promising novel treatment for hepatic encephalopathy Free Radic Biol Med
Molecular Hydrogen as a
novel antioxidant bull Akhavan O et al Hydrogen-rich water for green reduction of graphene oxide suspensions International Journal of Hydrogen Energy 2015 40(16) p 5553-
5560
bull 2Berjak P et al Cathodic amelioration of the adverse effects of oxidative stress accompanying procedures necessary for cryopreservation of embryonic
axes of recalcitrant-seeded species Seed Science Research 201121(3) p 187-203
bull 3Hanaoka K Antioxidant effects of reduced water produced by electrolysis of sodium chloride solutions Journal of Applied Electrochemistry 2001 31(12) p 1307-1313
bull 4Hanaoka K et al The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis Biophysical Chemistry 2004 107(1) p 71-82
bull 5Hiraoka A et al In Vitro Physicochemical Properties of Neutral Aqueous Solution Systems (Water Products as Drinks) Containing Hydrogen Gas 2-Carboxyethyl Germanium Sesquioxide and Platinum Nanocolloid as AdditivesJournal of Health Science 2010 56(2) p 167-174
bull 6Hiraoka A et al Studies on the properties and real existence of aqueous solution systems that are assumed to have antioxidant activities by the action of ldquoactive hydrogenrdquobdquo Journal of Health Science 2004 50(5) p 456-465
bull 7Kato S D Matsuoka and N Miwa Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR and 2 2prime-bipyridyl methods Materials Science and Engineering 2015 C 53 p 7-10
bull 8Lee MY et al Electrolyzed-reduced water protects against oxidative damage to DNA RNA and protein Appl Biochem Biotechnol 2006 135(2) p 133-44
bull 9Ohsawa I et al Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicalsNat Med 2007 13(6) p 688-694
bull 10Ohta S Molecular hydrogen as a novel antioxidant overview of the advantages of hydrogen for medical applications Methods Enzymol 2015 555 p 289-317
bull 11Park EJ et al Protective effect of electrolyzed reduced water on the paraquat-induced oxidative damage of human lymphocyte DNA Journal of the Korean Society for Applied Biological Chemistry 2005 48(2) p 155-160
bull 12Park SK et al Electrolyzed-reduced water confers increased resistance to environmental stresses Molecular amp Cellular Toxicology 2012 8(3) p 241-247
bull 13Park SK and SK Park Electrolyzed-reduced water increases resistance to oxidative stress fertility and lifespan via insulinIGF-1-like signal in C
elegans Biol Res 2013 46(2) p 147-52
bull 14Penders J R Kissner and WH Koppenol ONOOH does not react with H2 Free Radic Biol Med 2014
bull 15Qian L et al Administration of hydrogen-rich saline protects mice from lethal acute graft-versus-host disease (aGVHD) Transplantation 2013 95(5) p 658-62
bull 16Shi QH et al Hydrogen Therapy Reduces Oxidative Stress-associated Risks Following Acute and Chronic Exposure to High-altitude Environment Biomed Environ Sci 2015 28(3) p 239-41
bull 17Shirahata S et al Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage Biochemical and Biophysical Research Communications 1997 234(1) p 269-274
bull 18Yan H et al Mechanism of the lifespan extension of Caenorhabditis elegans by electrolyzed reduced waterndashparticipation of Pt nanoparticles Bioscience Biotechnology and Biochemistry 2011 75(7) p 1295-9
bull 19Yan H et al electrolyzed reduced water prolongs caenorhabditis elegans lifespan in Animal Cell Technology Basic amp Applied Aspects 2010 Springer Netherlands p 289-293
bull 20Yan HX et al Extension of the Lifespan of Caenorhabditis elegans by the Use of Electrolyzed Reduced Water Bioscience Biotechnology and Biochemistry 2010 74(10) p 2011-2015
Molecular Hydrogen
Research Human studies bull Aoki K et al Pilot study Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes Medical Gas Research
2012 2(1) p 12
bull 262Bittner AC et al Intra-Individual Ergonomics (I2E) Performance Effects of Ultra-Negative-Ion WaterProceedings of the Human Factors and Ergonomics
Society Annual Meeting SAGE Journals 2007 55(26) p 1617-1621
bull 263Drid P et al Hydrogen-Rich Water in Judo Training Psycho-Physiological Spiritual and Ethical Aspects) 2013 p 129
bull 264Fujiyama Y and T Kitahora Alkaline electrolytic water (alkali ions water) for drinking water in medicine Mizu no Tokusei to Atarashii Riyo Gijutsu Enu-Ti-Esu Tokyo 2004 p 348-457
bull 265Hiraoka A et al Effects of drinking a water product with anti-oxidant activities in vitro on the blood levels of biomarker substances for the oxidative stress Journal of Health Science 2006 52(6) p 817-820
bull 266Huang KC et al Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis Nephrology Dialysis Transplantation 2010 25(8) p 2730-2737
bull 267Huang KC et al Electrolyzed-reduced water reduced hemodialysis-induced erythrocyte impairment in end-stage renal disease patients Kidney Int 2006 70(2) p 391-8
bull 268Huang KC et al Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water Kidney Int 2003 64(2) p 704-14
bull 269Ishibashi T et al Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis an open-label pilot study Medical Gas Research 2012 2(1) p 27
bull 270Ishibashi T et al Therapeutic efficacy of infused molecular hydrogen in saline on rheumatoid arthritis A randomized double-blind placebo-controlled pilot study Int Immunopharmacol 2014 21(2) p 468-473
bull 271Ito M et al Open-label trial and randomized double-blind placebo-controlled crossover trial of hydrogen-enriched water for mitochondrial and inflammatory myopathies Medical Gas Research 2011 1(1) p 24
bull 272Kajiyama S et al Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance Nutrition Research 2008 28 p 137ndash143
bull 273Kang K-M et al Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors Medical Gas
Research 2011 1 p 11
bull 274Koyama K TY Saihara Y Ando D Goto Y Katayama A Effect of hydrogen saturated alkaline electrolyzed water on urinary oxidative stress markers after an acute exercise A randomized controlled trial Anti-aging Med 2008 4 p 117-122
bull 275Lee KJ et al Effect of electrolyzed-reduced water in vivo and in vitro examination and clinical trials in The 3rd Asia Pacific Conference on Evidence-Based Medicine 2004 Hong Kong
bull 276Li Q et al Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Med Gas Res 2013 3(1) p 20
bull 277Lu KC et al Electrolyzed reduced water attenuates hemodialysis-induced mononuclear cells apoptosis in end-stage renal disease patients Nephrology Dialysis Transplantation 2006 21 p 200-201
Molecular Hydrogen research
Human Studies cont bull Matsumoto S T Ueda and H Kakizaki Effect of supplementation with hydrogen-rich water in patients with interstitial cystitispainful bladder
syndrome Urology 2013 81(2) p 226-30
bull 279Nagatani K et al Safety of intravenous administration of hydrogen-enriched fluid in patients with acute cerebral ischemia initial clinical studies Med
Gas Res 2013 3 p 13
bull 280Nakao A et al Effectiveness of Hydrogen Rich Water on Antioxidant Status of Subjects with Potential Metabolic Syndrome-An Open Label Pilot Study Journal of Clinical Biochemistry and Nutrition 2010 46(2) p 140-149
bull 281Nakayama M et al Biological Effects of Electrolyzed Water in Hemodialysis Nephron Clinical Practice 2009112(1) p C9-C15
bull 282Nakayama M et al A novel bioactive haemodialysis system using dissolved dihydrogen (H-2) produced by water electrolysis a clinical trial Nephrology Dialysis Transplantation 2010 25(9) p 3026-3033
bull 283Ono H et al A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level Medical Gas Research 2012 2(1) p 21
bull 284Ono H et al Hydrogen(H2) treatment for acute erythymatous skin diseases A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers Medical Gas Research 2012 2(1) p 14
bull 285Ostojic SM Molecular Hydrogen in Sports Medicine New Therapeutic Perspectives Int J Sports Med 2014 (human)
bull 286Ostojic SM and MD Stojanovic Hydrogen-rich water affected blood alkalinity in physically active men Res Sports Med 2014 22(1) p 49-60
bull 287Ostojić SM et al Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women Double-blind randomized placebo-controlled cross-over trial of efficacy and safety Serbian journal of sports sciences 2011 5(1-4) p 83-89
bull 288Ostojic SM et al Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries Postgrad Med 2014 126(5) p 187-95
bull 289Shin MH et al Atomic Hydrogen Surrounded by Water Molecules H(H2O)m Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo PLoS One 2013 8(4) p e61696
bull 290Song G et al Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome Journal of Lipid Research 2013 54(7) p 1884-93
bull 291Takeuchi S et al Effects of intravenous infusion of hydrogen-rich fluid combined with intra-cisternal infusion of magnesium sulfate in severe aneurysmal subarachnoid hemorrhage study protocol for a randomized controlled trial BMC Neurol 2014 14(1) p 176
bull 292Tashiro H et al Clinical evaluation of alkali-ionized water for chronic diarrhea-placebo-controlled double blind study Digestion amp Absorption 2000 23 p 52-56
bull 293Terawaki H et al Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients a novel approach to suppress oxidative stress in the peritoneal cavity Medical Gas Research 2013 3(1) p 14
bull 294Xia C et al Effect of hydrogen-rich water on oxidative stress liver function and viral load in patients with chronic hepatitis B Clin Transl Sci 2013 6(5) p 372-5
bull 295Yang EJ et al A Clinical Trial of Orally Administered Alkaline Reduced Water 대한의생명과학회지 200713(2) p 83-89
bull 296Yeung LK et al Effect of electrolyzed reduced water hemodialysis on peripheral lymphocyte intracellular cytokine expression Nephrology Dialysis Transplantation 2006 21 p 204-204
bull 297Yoritaka A et al Pilot study of H(2) therapy in Parkinson‟s disease A randomized double-blind placebo-controlled trial Movement Disorders 2013
Molecular Hydrogen Functions
bull The most powerful selective Anti-oxidant (selective on hydroxyl radical and peroxynitrate) Does not disturb redox homeostasis ie doesn‟t reduce signalling molecules
bull Improves mitochondrial function increasing cell ATP energy production
bull Has Anti-inflammatory properties TNF ILs NF-Ks reduces cytokines etc
bull Has Anti-allergy functions stabilises mast cells bull Improved insulin sensitivity and glucose uptake
bull Cell signalling modulation via protein phosphorylation and regulates gene expression of pro-inflammatory cytokines and hormones likely via modulating peroxynitrite functions NFKB ILs TNF PGE2 FGF21 ghrelin
bull Up regulates other antioxidants including through NRF2 activity bull Passes across blood brain barrier
bull Alkalinising properties and enhancing (sparing) function of minerals
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen as a
novel antioxidant bull Akhavan O et al Hydrogen-rich water for green reduction of graphene oxide suspensions International Journal of Hydrogen Energy 2015 40(16) p 5553-
5560
bull 2Berjak P et al Cathodic amelioration of the adverse effects of oxidative stress accompanying procedures necessary for cryopreservation of embryonic
axes of recalcitrant-seeded species Seed Science Research 201121(3) p 187-203
bull 3Hanaoka K Antioxidant effects of reduced water produced by electrolysis of sodium chloride solutions Journal of Applied Electrochemistry 2001 31(12) p 1307-1313
bull 4Hanaoka K et al The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis Biophysical Chemistry 2004 107(1) p 71-82
bull 5Hiraoka A et al In Vitro Physicochemical Properties of Neutral Aqueous Solution Systems (Water Products as Drinks) Containing Hydrogen Gas 2-Carboxyethyl Germanium Sesquioxide and Platinum Nanocolloid as AdditivesJournal of Health Science 2010 56(2) p 167-174
bull 6Hiraoka A et al Studies on the properties and real existence of aqueous solution systems that are assumed to have antioxidant activities by the action of ldquoactive hydrogenrdquobdquo Journal of Health Science 2004 50(5) p 456-465
bull 7Kato S D Matsuoka and N Miwa Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR and 2 2prime-bipyridyl methods Materials Science and Engineering 2015 C 53 p 7-10
bull 8Lee MY et al Electrolyzed-reduced water protects against oxidative damage to DNA RNA and protein Appl Biochem Biotechnol 2006 135(2) p 133-44
bull 9Ohsawa I et al Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicalsNat Med 2007 13(6) p 688-694
bull 10Ohta S Molecular hydrogen as a novel antioxidant overview of the advantages of hydrogen for medical applications Methods Enzymol 2015 555 p 289-317
bull 11Park EJ et al Protective effect of electrolyzed reduced water on the paraquat-induced oxidative damage of human lymphocyte DNA Journal of the Korean Society for Applied Biological Chemistry 2005 48(2) p 155-160
bull 12Park SK et al Electrolyzed-reduced water confers increased resistance to environmental stresses Molecular amp Cellular Toxicology 2012 8(3) p 241-247
bull 13Park SK and SK Park Electrolyzed-reduced water increases resistance to oxidative stress fertility and lifespan via insulinIGF-1-like signal in C
elegans Biol Res 2013 46(2) p 147-52
bull 14Penders J R Kissner and WH Koppenol ONOOH does not react with H2 Free Radic Biol Med 2014
bull 15Qian L et al Administration of hydrogen-rich saline protects mice from lethal acute graft-versus-host disease (aGVHD) Transplantation 2013 95(5) p 658-62
bull 16Shi QH et al Hydrogen Therapy Reduces Oxidative Stress-associated Risks Following Acute and Chronic Exposure to High-altitude Environment Biomed Environ Sci 2015 28(3) p 239-41
bull 17Shirahata S et al Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage Biochemical and Biophysical Research Communications 1997 234(1) p 269-274
bull 18Yan H et al Mechanism of the lifespan extension of Caenorhabditis elegans by electrolyzed reduced waterndashparticipation of Pt nanoparticles Bioscience Biotechnology and Biochemistry 2011 75(7) p 1295-9
bull 19Yan H et al electrolyzed reduced water prolongs caenorhabditis elegans lifespan in Animal Cell Technology Basic amp Applied Aspects 2010 Springer Netherlands p 289-293
bull 20Yan HX et al Extension of the Lifespan of Caenorhabditis elegans by the Use of Electrolyzed Reduced Water Bioscience Biotechnology and Biochemistry 2010 74(10) p 2011-2015
Molecular Hydrogen
Research Human studies bull Aoki K et al Pilot study Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes Medical Gas Research
2012 2(1) p 12
bull 262Bittner AC et al Intra-Individual Ergonomics (I2E) Performance Effects of Ultra-Negative-Ion WaterProceedings of the Human Factors and Ergonomics
Society Annual Meeting SAGE Journals 2007 55(26) p 1617-1621
bull 263Drid P et al Hydrogen-Rich Water in Judo Training Psycho-Physiological Spiritual and Ethical Aspects) 2013 p 129
bull 264Fujiyama Y and T Kitahora Alkaline electrolytic water (alkali ions water) for drinking water in medicine Mizu no Tokusei to Atarashii Riyo Gijutsu Enu-Ti-Esu Tokyo 2004 p 348-457
bull 265Hiraoka A et al Effects of drinking a water product with anti-oxidant activities in vitro on the blood levels of biomarker substances for the oxidative stress Journal of Health Science 2006 52(6) p 817-820
bull 266Huang KC et al Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis Nephrology Dialysis Transplantation 2010 25(8) p 2730-2737
bull 267Huang KC et al Electrolyzed-reduced water reduced hemodialysis-induced erythrocyte impairment in end-stage renal disease patients Kidney Int 2006 70(2) p 391-8
bull 268Huang KC et al Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water Kidney Int 2003 64(2) p 704-14
bull 269Ishibashi T et al Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis an open-label pilot study Medical Gas Research 2012 2(1) p 27
bull 270Ishibashi T et al Therapeutic efficacy of infused molecular hydrogen in saline on rheumatoid arthritis A randomized double-blind placebo-controlled pilot study Int Immunopharmacol 2014 21(2) p 468-473
bull 271Ito M et al Open-label trial and randomized double-blind placebo-controlled crossover trial of hydrogen-enriched water for mitochondrial and inflammatory myopathies Medical Gas Research 2011 1(1) p 24
bull 272Kajiyama S et al Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance Nutrition Research 2008 28 p 137ndash143
bull 273Kang K-M et al Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors Medical Gas
Research 2011 1 p 11
bull 274Koyama K TY Saihara Y Ando D Goto Y Katayama A Effect of hydrogen saturated alkaline electrolyzed water on urinary oxidative stress markers after an acute exercise A randomized controlled trial Anti-aging Med 2008 4 p 117-122
bull 275Lee KJ et al Effect of electrolyzed-reduced water in vivo and in vitro examination and clinical trials in The 3rd Asia Pacific Conference on Evidence-Based Medicine 2004 Hong Kong
bull 276Li Q et al Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Med Gas Res 2013 3(1) p 20
bull 277Lu KC et al Electrolyzed reduced water attenuates hemodialysis-induced mononuclear cells apoptosis in end-stage renal disease patients Nephrology Dialysis Transplantation 2006 21 p 200-201
Molecular Hydrogen research
Human Studies cont bull Matsumoto S T Ueda and H Kakizaki Effect of supplementation with hydrogen-rich water in patients with interstitial cystitispainful bladder
syndrome Urology 2013 81(2) p 226-30
bull 279Nagatani K et al Safety of intravenous administration of hydrogen-enriched fluid in patients with acute cerebral ischemia initial clinical studies Med
Gas Res 2013 3 p 13
bull 280Nakao A et al Effectiveness of Hydrogen Rich Water on Antioxidant Status of Subjects with Potential Metabolic Syndrome-An Open Label Pilot Study Journal of Clinical Biochemistry and Nutrition 2010 46(2) p 140-149
bull 281Nakayama M et al Biological Effects of Electrolyzed Water in Hemodialysis Nephron Clinical Practice 2009112(1) p C9-C15
bull 282Nakayama M et al A novel bioactive haemodialysis system using dissolved dihydrogen (H-2) produced by water electrolysis a clinical trial Nephrology Dialysis Transplantation 2010 25(9) p 3026-3033
bull 283Ono H et al A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level Medical Gas Research 2012 2(1) p 21
bull 284Ono H et al Hydrogen(H2) treatment for acute erythymatous skin diseases A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers Medical Gas Research 2012 2(1) p 14
bull 285Ostojic SM Molecular Hydrogen in Sports Medicine New Therapeutic Perspectives Int J Sports Med 2014 (human)
bull 286Ostojic SM and MD Stojanovic Hydrogen-rich water affected blood alkalinity in physically active men Res Sports Med 2014 22(1) p 49-60
bull 287Ostojić SM et al Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women Double-blind randomized placebo-controlled cross-over trial of efficacy and safety Serbian journal of sports sciences 2011 5(1-4) p 83-89
bull 288Ostojic SM et al Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries Postgrad Med 2014 126(5) p 187-95
bull 289Shin MH et al Atomic Hydrogen Surrounded by Water Molecules H(H2O)m Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo PLoS One 2013 8(4) p e61696
bull 290Song G et al Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome Journal of Lipid Research 2013 54(7) p 1884-93
bull 291Takeuchi S et al Effects of intravenous infusion of hydrogen-rich fluid combined with intra-cisternal infusion of magnesium sulfate in severe aneurysmal subarachnoid hemorrhage study protocol for a randomized controlled trial BMC Neurol 2014 14(1) p 176
bull 292Tashiro H et al Clinical evaluation of alkali-ionized water for chronic diarrhea-placebo-controlled double blind study Digestion amp Absorption 2000 23 p 52-56
bull 293Terawaki H et al Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients a novel approach to suppress oxidative stress in the peritoneal cavity Medical Gas Research 2013 3(1) p 14
bull 294Xia C et al Effect of hydrogen-rich water on oxidative stress liver function and viral load in patients with chronic hepatitis B Clin Transl Sci 2013 6(5) p 372-5
bull 295Yang EJ et al A Clinical Trial of Orally Administered Alkaline Reduced Water 대한의생명과학회지 200713(2) p 83-89
bull 296Yeung LK et al Effect of electrolyzed reduced water hemodialysis on peripheral lymphocyte intracellular cytokine expression Nephrology Dialysis Transplantation 2006 21 p 204-204
bull 297Yoritaka A et al Pilot study of H(2) therapy in Parkinson‟s disease A randomized double-blind placebo-controlled trial Movement Disorders 2013
Molecular Hydrogen Functions
bull The most powerful selective Anti-oxidant (selective on hydroxyl radical and peroxynitrate) Does not disturb redox homeostasis ie doesn‟t reduce signalling molecules
bull Improves mitochondrial function increasing cell ATP energy production
bull Has Anti-inflammatory properties TNF ILs NF-Ks reduces cytokines etc
bull Has Anti-allergy functions stabilises mast cells bull Improved insulin sensitivity and glucose uptake
bull Cell signalling modulation via protein phosphorylation and regulates gene expression of pro-inflammatory cytokines and hormones likely via modulating peroxynitrite functions NFKB ILs TNF PGE2 FGF21 ghrelin
bull Up regulates other antioxidants including through NRF2 activity bull Passes across blood brain barrier
bull Alkalinising properties and enhancing (sparing) function of minerals
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen
Research Human studies bull Aoki K et al Pilot study Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes Medical Gas Research
2012 2(1) p 12
bull 262Bittner AC et al Intra-Individual Ergonomics (I2E) Performance Effects of Ultra-Negative-Ion WaterProceedings of the Human Factors and Ergonomics
Society Annual Meeting SAGE Journals 2007 55(26) p 1617-1621
bull 263Drid P et al Hydrogen-Rich Water in Judo Training Psycho-Physiological Spiritual and Ethical Aspects) 2013 p 129
bull 264Fujiyama Y and T Kitahora Alkaline electrolytic water (alkali ions water) for drinking water in medicine Mizu no Tokusei to Atarashii Riyo Gijutsu Enu-Ti-Esu Tokyo 2004 p 348-457
bull 265Hiraoka A et al Effects of drinking a water product with anti-oxidant activities in vitro on the blood levels of biomarker substances for the oxidative stress Journal of Health Science 2006 52(6) p 817-820
bull 266Huang KC et al Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis Nephrology Dialysis Transplantation 2010 25(8) p 2730-2737
bull 267Huang KC et al Electrolyzed-reduced water reduced hemodialysis-induced erythrocyte impairment in end-stage renal disease patients Kidney Int 2006 70(2) p 391-8
bull 268Huang KC et al Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water Kidney Int 2003 64(2) p 704-14
bull 269Ishibashi T et al Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis an open-label pilot study Medical Gas Research 2012 2(1) p 27
bull 270Ishibashi T et al Therapeutic efficacy of infused molecular hydrogen in saline on rheumatoid arthritis A randomized double-blind placebo-controlled pilot study Int Immunopharmacol 2014 21(2) p 468-473
bull 271Ito M et al Open-label trial and randomized double-blind placebo-controlled crossover trial of hydrogen-enriched water for mitochondrial and inflammatory myopathies Medical Gas Research 2011 1(1) p 24
bull 272Kajiyama S et al Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance Nutrition Research 2008 28 p 137ndash143
bull 273Kang K-M et al Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors Medical Gas
Research 2011 1 p 11
bull 274Koyama K TY Saihara Y Ando D Goto Y Katayama A Effect of hydrogen saturated alkaline electrolyzed water on urinary oxidative stress markers after an acute exercise A randomized controlled trial Anti-aging Med 2008 4 p 117-122
bull 275Lee KJ et al Effect of electrolyzed-reduced water in vivo and in vitro examination and clinical trials in The 3rd Asia Pacific Conference on Evidence-Based Medicine 2004 Hong Kong
bull 276Li Q et al Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Med Gas Res 2013 3(1) p 20
bull 277Lu KC et al Electrolyzed reduced water attenuates hemodialysis-induced mononuclear cells apoptosis in end-stage renal disease patients Nephrology Dialysis Transplantation 2006 21 p 200-201
Molecular Hydrogen research
Human Studies cont bull Matsumoto S T Ueda and H Kakizaki Effect of supplementation with hydrogen-rich water in patients with interstitial cystitispainful bladder
syndrome Urology 2013 81(2) p 226-30
bull 279Nagatani K et al Safety of intravenous administration of hydrogen-enriched fluid in patients with acute cerebral ischemia initial clinical studies Med
Gas Res 2013 3 p 13
bull 280Nakao A et al Effectiveness of Hydrogen Rich Water on Antioxidant Status of Subjects with Potential Metabolic Syndrome-An Open Label Pilot Study Journal of Clinical Biochemistry and Nutrition 2010 46(2) p 140-149
bull 281Nakayama M et al Biological Effects of Electrolyzed Water in Hemodialysis Nephron Clinical Practice 2009112(1) p C9-C15
bull 282Nakayama M et al A novel bioactive haemodialysis system using dissolved dihydrogen (H-2) produced by water electrolysis a clinical trial Nephrology Dialysis Transplantation 2010 25(9) p 3026-3033
bull 283Ono H et al A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level Medical Gas Research 2012 2(1) p 21
bull 284Ono H et al Hydrogen(H2) treatment for acute erythymatous skin diseases A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers Medical Gas Research 2012 2(1) p 14
bull 285Ostojic SM Molecular Hydrogen in Sports Medicine New Therapeutic Perspectives Int J Sports Med 2014 (human)
bull 286Ostojic SM and MD Stojanovic Hydrogen-rich water affected blood alkalinity in physically active men Res Sports Med 2014 22(1) p 49-60
bull 287Ostojić SM et al Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women Double-blind randomized placebo-controlled cross-over trial of efficacy and safety Serbian journal of sports sciences 2011 5(1-4) p 83-89
bull 288Ostojic SM et al Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries Postgrad Med 2014 126(5) p 187-95
bull 289Shin MH et al Atomic Hydrogen Surrounded by Water Molecules H(H2O)m Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo PLoS One 2013 8(4) p e61696
bull 290Song G et al Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome Journal of Lipid Research 2013 54(7) p 1884-93
bull 291Takeuchi S et al Effects of intravenous infusion of hydrogen-rich fluid combined with intra-cisternal infusion of magnesium sulfate in severe aneurysmal subarachnoid hemorrhage study protocol for a randomized controlled trial BMC Neurol 2014 14(1) p 176
bull 292Tashiro H et al Clinical evaluation of alkali-ionized water for chronic diarrhea-placebo-controlled double blind study Digestion amp Absorption 2000 23 p 52-56
bull 293Terawaki H et al Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients a novel approach to suppress oxidative stress in the peritoneal cavity Medical Gas Research 2013 3(1) p 14
bull 294Xia C et al Effect of hydrogen-rich water on oxidative stress liver function and viral load in patients with chronic hepatitis B Clin Transl Sci 2013 6(5) p 372-5
bull 295Yang EJ et al A Clinical Trial of Orally Administered Alkaline Reduced Water 대한의생명과학회지 200713(2) p 83-89
bull 296Yeung LK et al Effect of electrolyzed reduced water hemodialysis on peripheral lymphocyte intracellular cytokine expression Nephrology Dialysis Transplantation 2006 21 p 204-204
bull 297Yoritaka A et al Pilot study of H(2) therapy in Parkinson‟s disease A randomized double-blind placebo-controlled trial Movement Disorders 2013
Molecular Hydrogen Functions
bull The most powerful selective Anti-oxidant (selective on hydroxyl radical and peroxynitrate) Does not disturb redox homeostasis ie doesn‟t reduce signalling molecules
bull Improves mitochondrial function increasing cell ATP energy production
bull Has Anti-inflammatory properties TNF ILs NF-Ks reduces cytokines etc
bull Has Anti-allergy functions stabilises mast cells bull Improved insulin sensitivity and glucose uptake
bull Cell signalling modulation via protein phosphorylation and regulates gene expression of pro-inflammatory cytokines and hormones likely via modulating peroxynitrite functions NFKB ILs TNF PGE2 FGF21 ghrelin
bull Up regulates other antioxidants including through NRF2 activity bull Passes across blood brain barrier
bull Alkalinising properties and enhancing (sparing) function of minerals
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen research
Human Studies cont bull Matsumoto S T Ueda and H Kakizaki Effect of supplementation with hydrogen-rich water in patients with interstitial cystitispainful bladder
syndrome Urology 2013 81(2) p 226-30
bull 279Nagatani K et al Safety of intravenous administration of hydrogen-enriched fluid in patients with acute cerebral ischemia initial clinical studies Med
Gas Res 2013 3 p 13
bull 280Nakao A et al Effectiveness of Hydrogen Rich Water on Antioxidant Status of Subjects with Potential Metabolic Syndrome-An Open Label Pilot Study Journal of Clinical Biochemistry and Nutrition 2010 46(2) p 140-149
bull 281Nakayama M et al Biological Effects of Electrolyzed Water in Hemodialysis Nephron Clinical Practice 2009112(1) p C9-C15
bull 282Nakayama M et al A novel bioactive haemodialysis system using dissolved dihydrogen (H-2) produced by water electrolysis a clinical trial Nephrology Dialysis Transplantation 2010 25(9) p 3026-3033
bull 283Ono H et al A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level Medical Gas Research 2012 2(1) p 21
bull 284Ono H et al Hydrogen(H2) treatment for acute erythymatous skin diseases A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers Medical Gas Research 2012 2(1) p 14
bull 285Ostojic SM Molecular Hydrogen in Sports Medicine New Therapeutic Perspectives Int J Sports Med 2014 (human)
bull 286Ostojic SM and MD Stojanovic Hydrogen-rich water affected blood alkalinity in physically active men Res Sports Med 2014 22(1) p 49-60
bull 287Ostojić SM et al Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women Double-blind randomized placebo-controlled cross-over trial of efficacy and safety Serbian journal of sports sciences 2011 5(1-4) p 83-89
bull 288Ostojic SM et al Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries Postgrad Med 2014 126(5) p 187-95
bull 289Shin MH et al Atomic Hydrogen Surrounded by Water Molecules H(H2O)m Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo PLoS One 2013 8(4) p e61696
bull 290Song G et al Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome Journal of Lipid Research 2013 54(7) p 1884-93
bull 291Takeuchi S et al Effects of intravenous infusion of hydrogen-rich fluid combined with intra-cisternal infusion of magnesium sulfate in severe aneurysmal subarachnoid hemorrhage study protocol for a randomized controlled trial BMC Neurol 2014 14(1) p 176
bull 292Tashiro H et al Clinical evaluation of alkali-ionized water for chronic diarrhea-placebo-controlled double blind study Digestion amp Absorption 2000 23 p 52-56
bull 293Terawaki H et al Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients a novel approach to suppress oxidative stress in the peritoneal cavity Medical Gas Research 2013 3(1) p 14
bull 294Xia C et al Effect of hydrogen-rich water on oxidative stress liver function and viral load in patients with chronic hepatitis B Clin Transl Sci 2013 6(5) p 372-5
bull 295Yang EJ et al A Clinical Trial of Orally Administered Alkaline Reduced Water 대한의생명과학회지 200713(2) p 83-89
bull 296Yeung LK et al Effect of electrolyzed reduced water hemodialysis on peripheral lymphocyte intracellular cytokine expression Nephrology Dialysis Transplantation 2006 21 p 204-204
bull 297Yoritaka A et al Pilot study of H(2) therapy in Parkinson‟s disease A randomized double-blind placebo-controlled trial Movement Disorders 2013
Molecular Hydrogen Functions
bull The most powerful selective Anti-oxidant (selective on hydroxyl radical and peroxynitrate) Does not disturb redox homeostasis ie doesn‟t reduce signalling molecules
bull Improves mitochondrial function increasing cell ATP energy production
bull Has Anti-inflammatory properties TNF ILs NF-Ks reduces cytokines etc
bull Has Anti-allergy functions stabilises mast cells bull Improved insulin sensitivity and glucose uptake
bull Cell signalling modulation via protein phosphorylation and regulates gene expression of pro-inflammatory cytokines and hormones likely via modulating peroxynitrite functions NFKB ILs TNF PGE2 FGF21 ghrelin
bull Up regulates other antioxidants including through NRF2 activity bull Passes across blood brain barrier
bull Alkalinising properties and enhancing (sparing) function of minerals
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen Functions
bull The most powerful selective Anti-oxidant (selective on hydroxyl radical and peroxynitrate) Does not disturb redox homeostasis ie doesn‟t reduce signalling molecules
bull Improves mitochondrial function increasing cell ATP energy production
bull Has Anti-inflammatory properties TNF ILs NF-Ks reduces cytokines etc
bull Has Anti-allergy functions stabilises mast cells bull Improved insulin sensitivity and glucose uptake
bull Cell signalling modulation via protein phosphorylation and regulates gene expression of pro-inflammatory cytokines and hormones likely via modulating peroxynitrite functions NFKB ILs TNF PGE2 FGF21 ghrelin
bull Up regulates other antioxidants including through NRF2 activity bull Passes across blood brain barrier
bull Alkalinising properties and enhancing (sparing) function of minerals
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen Molecular Hydrogen(H2) is a molecule composed of two hydrogen
atoms bonded to each other This molecule does not commonly
occur in nature Most of the time hydrogen atoms bond to other
atoms For example two hydrogen atoms are bonded to a single
oxygen atom to form a water molecule
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Periodic Table
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen and Health
bull Hydrogen (H) appears in the top left corner of the periodic table and is
denoted as 1 It contains one proton and one electron with no neutrons This
means that the element hydrogen is the smallest simplest and most
fundamental element This makes Molecular Hydrogen (H2) the simplest
molecule in existence since itrsquos a bond of two of the smallest atoms
Why have the benefits of Molecular Hydrogen not been promoted more heavily
The benefits of H2 are hard to explain to the average person because ldquoitrsquos too
simplerdquo H2 canrsquot be patented hence itrsquos not going to be profitable as a
pharmaceutical drug Therefore not many resources have been allocated to
promote H2 in mainstream media Also H2 seems to be ldquogood for everythingrdquo at
first glance Since H2 works on the antioxidant defence system inside our cells
its effects vary from person to person Free radicals affect each person in
different ways This can be a difficult concept to grasp for some people and
sounds ldquotoo good to be truerdquo
Despite these hurdles progressively more academics and researchers have
documented the undeniable evidence for the benefits of H2 on human health The
research on molecular hydrogen has grown exponentially over the past 10
years
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen is the
Perfect Antioxidant bull No other antioxidants have the ability to act in the
multiple ways that Molecular Hydrogen (H2) does to
protect from oxidative stress H2 is the Perfect
Antioxidant
bull Zero Toxicity
bull Size
bull Selectivity
bull Synergy
bull Other non-antioxidant added benefits ie anti-
inflammatory cell signalling modulation etc
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Why is Molecular Hydrogen So Effective ndash Zero Toxicity Hydrogen neutralizes free radicals and turns it into water leaving no
byproducts(Other antioxidants turn into weaker free radicals themselves after neutralizing free radicals) The Hydroxyl Radical (bullOH) is the most dangerous
free radical because it directly attacks and damages cells proteins DNA and
Lipids
Notice how Ascorbic Acid
(Vitamin C) becomes an Ascorbyl
Radical once it reacts with Rbull
(Free Radical) Therefore even
though Vitamin C and other
antioxidants neutralize free
radicals they become weak free
radicals themselves
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen is so Effective due to its Size
Molecular Hydrogen is the smallest molecule in existence Because hydrogen is so small it can penetrate deep into the cells to eliminate free radicals at their source in the mitochondria (Representation of the molecular weights of different antioxidants vs hydrogen shown below) Also due to its size hydrogen can easily cross the blood-brain-barrier to eliminate free radicals in the brain
Hydrogen 2
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Free Radicals and Selectivity
Low levels of superoxide and
hydrogen peroxide are beneficial
They signal adaptation to stress and
protect the cell from infections and
themselves have antimicrobial properties
Important to not disrupt these H2O2 regulates cell differentiation the immune
stystem autophagy and apoptosis
Molecular Hydrogen binds
To OH- and turns it into water
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Why is Molecular Hydrogen So Effective ndash Synergy
Hydrogen upregulates antioxidant systems that are already present inside our cells Our cells contain antioxidant systems such as Superoxide Dismutase and Glutathione Peroxidase that neutralize free radicals such as the Superoxide Anion (O2
-) and Hydrogen Peroxide (H2O2) an excess of which converts into the harmful Hydroxyl Radicals Hydrogen increases the activity of these endogenous antioxidants Link httpbitly1hZhqmJ
H2 Upregulates These Antioxidant Enzymes
Molecular Hydrogen Increases the Activity of Endogenous Antioxidants (Source http1usagov1nqxK88)
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Most Common Chronic Health
Conditions bull Heart disease
bull Stroke
bull Cancer
bull Mental Health
bull Diabetes
bull Obesity
bull Alzheimerrsquos Disease
bull Arthritis
bull Osteoporosis
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Disease is caused by lack of cell energy or
oxidative tissue damage
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Where we create energy
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
When Mitochondria Generates ATP Free Radicals are produced as a byproduct of metabolism
Damages DNA Lipids Proteins etc
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
What Are Free Radicals (Cause
Oxidative Stress)
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Oxidative Stress (Inflammation)
Causes Disease and Aging
Oxidative stress has been linked to 90 of all diseases as well as aging As shown in the figure below oxidative stress is the underlying cause of diseases such as cancer diabetes Alzheimer‟s Parkinson‟s Disease etc You can find journals that link oxidative stress to each disease mentioned online
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Oxidation Inflammation and
Disease Are All Related
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Oxidation and Inflammation
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
How Oxidative Stress
Causes Disease
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Cell Membrane Transportation When membranes are damaged nutrients and toxins
cannot get transported in or out
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
When mitochondrial
membranes are damaged they
can‟t make ATP efficiently
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
DNA Damage
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Calcium Regulation Calcium channels on the cell
membrane open in response to a
stress trigger increasing the flow of
calcium ions into the cell This
calcium triggers excitatory
mechanisms to increase the activity
of the cell in response to a stress
This increased metabolic activity in
the cell dramatically increases
oxidative stress which in turn
damages the cell and causes
disease
This is responsible for reperfusion
injury which is dramatically reduced
by the presence of molecular
hydrogen
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Research for Molecular Hydrogen and Diabetes Therapy
bull Kajiyama S Hasegawa G Asano M Hosoda H Fukui M Nakamura N et al
Supplementation of hydrogen rich water improves lipid and glucose
metabolism in patients with type 2 diabetes or impaired glucose tolerance
Nutr Res 2008 28137-43
bull Wang QJ et al Therapeutic effects of hydrogen saturated saline on rat
diabetic model and insulin resistant model via reduction of oxidative
stress Chin Med J (Engl) 2012 125(9) p 1633-7
bull Amitani H et al Hydrogen Improves Glycemic Control in Type1 Diabetic
Animal Model by Promoting Glucose Uptake into Skeletal Muscle PLoS One
2013 8(1)
bull Kamimura N et al Molecular Hydrogen Improves Obesity and Diabetes by
Inducing Hepatic FGF21 and Stimulating Energy Metabolism in dbdb
Mice Obesity 2011
bull Yeunhwa GU KO Taigo FUj Yuka ITOKAWA et al Anti Type 2 Diabetic Effect
and Anti-oxidation Effect in Active Hydrogen Water Administration KK-Ay
Mice Medicine and Biology 2006 150(11) p 384-392
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
bull Improved insulin function and reduced plasma glucose and
TG increased uptake into skeletal muscle
bull Reduced oxidative stress related tissue damage with inhibition
of TNF induced monocyte adhesion to endothelial cells and
endothelium protection from TNF induced apoptosis
bull Decreased levels of total and oxidised LDL free fatty acids
and lipid peroxidation and raised HDL after 8-10 weeks
bull Improved fatty liver disease in dbdb mice (type 2 diabetes
mice model)
bull Increasing levels of plasma adiponectin and superoxide
dismutase
bull In 23 of patients with IGT molecular hydrogen normalised oral glucose tolerance test
35
Effects of Molecular Hydrogen on Diabetes
And Metabolic Syndrome
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
The Vicious Cycle of Insulin Resistance
Hyperglycemia causes tissue damage through multiple mechanisms including increased flux of glucose and other
sugars through the polyol pathway increased intracellular formation of advanced glycation end products (AGEs)
increased expression of the receptor for AGEs and its activating ligands activation of protein kinase C isoforms and
over activity of the hexosamine pathway All of these pathways contribute to increased free radical production (O2-)
and eventually causes insulin resistance Beta-Cell dysfunction and diabetic complications [Source]
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
It is more than bdquoHigh GI‟ food choices that cause
metabolic syndrome
Chronic oxidative stress causes mitochondrial
damage causing insulin resistance
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Mitochondrial damage
causing insulin resistance
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
ATP
ADP
K+
Ca++
Insulin
[1] [2]
[3]
[4]
GLUT2
Pancreatic Beta Cell
Beta-cell mitochondria serve as fuel sensors that link glucose exposure to insulin release 1) GLUT2 transporters
introduce glucose to mitochondria causing increased ATPADP ratio
2) K+ gate closure--gt depolarization
3) Voltage sensitive Ca++ channels open
4) Exocytosis of insulin storage granules to bloodstream
Pancreatic beta cells have one of the lowest levels of antioxidants in the body and
hence are especially susceptible to damage by free radicals which effects their
ability to release insulin
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Diabetes Reducing
Complications Risk by Reducing
Oxidative Stress
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular hydrogen and brain
protection bull Bari F et al Inhalation of Hydrogen Gas Protects Cerebrovascular Reactivity Against Moderate but Not Severe Perinatal Hypoxic Injury in Newborn
Piglets Stroke 2010 41(4) p E323-E323
bull 36Cui Y et al Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats J Surg Res 2014
bull 37Dohi K et al Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury PLoS One 2014 9(9) p e108034
bull 38Domoki F et al Hydrogen is Neuroprotective and Preserves Cerebrovascular Reactivity in Asphyxiated Newborn Pigs Pediatric Research 2010 68(5) p 387-392
bull 39Eckermann JM et al Hydrogen is neuroprotective against surgically induced brain injury Medical Gas Research 2011 1(1) p 7
bull 40Feng Y et al Hydrogen-rich saline prevents early neurovascular dysfunction resulting from inhibition of oxidative stress in STZ-diabetic rats Curr Eye Res 2013 38(3) p 396-404
bull 41Fu Y et al Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson‟s disease Neuroscience Letters 2009 453 p 81ndash85
bull 42Fujita K et al Hydrogen in drinking water reduces dopaminergic neuronal loss in the 1-methyl-4-phenyl-1236-tetrahydropyridine mouse model of Parkinson‟s disease PLoS One 2009 4(9) p e7247
bull 43Gu Y et al Drinking Hydrogen Water Ameliorated Cognitive Impairment in Senescence-Accelerated MiceJournal of Clinical Biochemistry and Nutrition 2010 46(3) p 269-276
bull 44Han L et al Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins Brain Res 2015
bull 45Hong Y et al Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats J Neurosci Res 2012 90(8) p 1670-80
bull 46Hong Y et al Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage possible role of the AktGSK3beta signaling pathway PLoS One 2014 9(4) p e96212
bull 47Hou Z et al Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic brain injury Brain Res Bull 2012 88(6) p 560-5
bull 48Huang G et al The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation 2013 84(5) p 690-5
bull 49Hugyecz M et al Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus Brain Research 2011 1404 p 31-8
bull 50Ito M et al Drinking hydrogen water and intermittent hydrogen gas exposure but not lactulose or continuous hydrogen gas exposure prevent 6-hydorxydopamine-induced Parkinson‟s disease in rats Med Gas Res 20122(1) p 15
bull 51Ji X et al Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress Brain Research 2010 1354 p 196-205
bull 52Ji X et al Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress Journal of Surgical Research 2012 178(1) p e9-16
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular hydrogen and
brain protection cont bull Kashiwagi T et al Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water Animal Cell Technology Meets
Genomics 2005 2 p 257-260
bull 54Kashiwagi T et al Electrochemically reduced water protects neural cells from oxidative damage Oxid Med Cell Longev 2014 2014 p 869121
bull 55Kobayashi H et al Effects of Hydrogen Gas in a Mouse Cold Induced Brain Injury Model Journal of Neurotrauma 2011 28(5) p A64-A64
bull 56Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models alpha P-31-NMR study Neuroscience Research 2009 65 p S124-S124
bull 57Kuroki C et al Neuroprotective effects of hydrogen gas on brain in three types of stress models A P-31-NMR and ESR study Neuroscience Research 2011 71 p E406-E406
bull 58Li J et al Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer‟s disease by reduction of oxidative stress Brain Res 2010 1328 p 152-161
bull 59Liu FT et al Molecular Hydrogen Suppresses Reactive Astrogliosis Related to Oxidative Injury during Spinal Cord Injury in Rats CNS Neurosci Ther 2014
bull 60Liu L et al Inhalation of hydrogen gas attenuates brain injury in mice with cecal ligation and puncture via inhibiting neuroinflammation oxidative stress and neuronal apoptosis Brain Res 2014 1589 p 78-92
bull 61Liu W et al Protective effects of hydrogen on fetal brain injury during maternal hypoxia Acta Neurochir Suppl 2011 111 p 307-11
bull 62Manaenko A et al Hydrogen inhalation is neuroprotective and improves functional outcomes in mice after intracerebral hemorrhage Acta Neurochir Suppl 2011 111 p 179-83
bull 63Manaenko A et al Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice Critical Care Medicine 2013 41(5) p 1266-75
bull 64Mano Y et al Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion Free Radic Biol Med 2014 69 p 324-30
bull 65Matsumoto A et al Oral bdquohydrogen water‟ induces neuroprotective ghrelin secretion in mice Sci Rep 2013 3 p 3273
bull 66Mei K et al Hydrogen protects rats from dermatitis caused by local radiation J Dermatolog Treat 201425(2) p 182-8
bull 67Nagata K et al Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice Neuropsychopharmacology 2009 34(2) p 501-508
bull 68Olah O et al Delayed neurovascular dysfunction is alleviated by hydrogen in asphyxiated newborn pigsNeonatology 2013 104(2) p 79-86
bull 69Ono H et al Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers Edaravone and hydrogen as compared to Edaravone alone A non-controlled study Medical Gas Research 2011 1(1) p 12
bull 70Ostojic SM Targeting molecular hydrogen to mitochondria Barriers and gateways Pharmacol Res 2015 94 p 51-3 (brain)
bull 71Pshenichnyuk SA and AS Komolov Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria The Journal of Physical Chemistry Letters 20156(7) p 1104-1110
bull 72Sato Y et al Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30GNL knockout mice Biochem Biophys Res Commun 2008 375(3) p 346-350
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen and Athletes bull Reduces oxidative stress protecting cells and
mitochondrial function
bull Has anti-inflammatory properties that enhance
tissue repair after training
bull Reduces lactic acid build up and increases
bicarbonate levels during exercise
bull Increases muscle energy production by increasing
measurable muscle torque during isotonic exercise
bull Reduces free radical DNA damage substrates post
exercise
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Oxidative Stress is Linked to Fatigue and Inflammation
Enemies of the Athlete (Research)
ldquoHigh levels of reactive oxygen species (Free Radicals) promote
contractile dysfunction resulting in muscle weakness and fatiguerdquo
Link httpbitlySeMRDJ
Oxidative stress causes inflammation and inflammation in turn causes
more oxidative stress This cycle occurs because oxidation causes
various protein dysfunctions and that hinders the operation of functions
that restore a healthy oxidantantioxidant balance
Link httpbitlySeOXDx
However ldquoHeavy and sustained exercise training generates large
quantities of free-radicals that likely outstrip the buffering capacity of
the system leaving these individuals susceptible to oxidative stresshelliprdquo
writes Harshal R Patil MD
Link httpbitly1i0o0ta
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen for
Athletes - Research
Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes
Author Aoki et al 2012 Link http1usagov1hRNfQx Hydrogen water decreased the
elevation of blood lactate during heavy exercise Furthermore it inhibited the early decrease in peak torque during maximal isokinetic knee extension Athletes consuming regular water did not experience this inhibition
HW
Hydrogen
Water
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen for Athletes - Research
A way of using hydrogen-
containing water for athletes Author Noriki Nagao MD PhD
Link httpbitly1hYUIva
Consumption of hydrogen-containing water
before exercise reduces the level of
8-OHdG(breakdown product of free radical
induced DNA damage) excreted in urine
by 20 compared with consumption of mineral
water
Therefore consumption of hydrogen containing
water before exercise reduces DNA
damage caused by reactive oxygen species
(hydroxyl radical)
Hydrogen-containing water is an efficient
method to reduce DNA damage and fatigue
caused by physical stress and extreme training
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen for Athletes - Research
Hydrogen-Rich Water Affected Blood Alkalinity
in Physically Active Men Author SERGEJ M OSTOJIC and MARKO D
STOJANOVIC (2014) Link http1usagov1f4ts0C The hydrogen water supplemented group had significant increases in fasting arterial blood pH (004) and serum bicarbonates (25 μmolL) as compared with the baseline after 14 days of intervention A significant increase
in postexercise pH (007) was also observed bull Higher post exercise pH potentially indicates a better environment for repeated muscle contraction as water buffers inorganic cations generated by exercise bull No volunteers withdrew before the end of the study and no participant reported any
vexatious side effects of supplementation bull These results support the hypothesis that hydrogen water administration is safe and may have an alkalizing effect in young physically active men
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Oxidative stress linked to
Chronic Fatigue
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
49
bull UVA radiation contributes to skin aging via the induction of collagenase mRNA (a matrix metalloproteinase enzyme) which increases ROS production and reduces Type I collagen in fibroblasts and keratinocytes
Degradation of collagen type I in the dermis leads to formation of wrinkles bull Consuming hydrogen water significantly increased human embryo fibroblast Type I collagen production bull Hydrogen water was found to significantly suppress UVA-induced reactive oxygen species in OUMS-36 fibroblasts bull Consuming hydrogen water prior to UVA irradiation increased cell viability from 29 to 35 In human keratinocytes bull Subjects bathing in hydrogen water displayed a significant decrease in wrinkle areas across the body
bull Therefore warming hydrogen water to 41C dilated hair follicles and allowed hydrogen to penetrate the dermis where it decreased intracellular levels of ROS induced by irradiation This process inhibited cell death and DNA damage as well as promoted type I collagen production
Summary
Efficacy of Molecular Hydrogen for Skin
Journal of Photochemistry and Photobiology B Biology 2011Oct 20 Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-1
collagen production and oxidative-stress diminishment in fibroblast and cell-injury prevention in keratinocytes
Kato S Saitoh Y Iwai K Miwa N (Laboratory of Cell-Death Control BioTechnology Faculty of Life and Environmental Sciences Prefectural University of Hiroshima Nanatsuka 562 Shobara Hiroshima 727-0023 Japan) Link http1usagov1gdEE78
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
50
Medical Gas Research 2013 320 Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro Kosuke Aoki Atsunori Nakao Takako Adachi Yasushi Matsui1 and Shumpei Miyakawa Link httpbitlyP6BwUQ
Summary
Administering hydrogen water to hospitalized elderly
patients with pressure ulcers showed a decrease in
wound size (914 reduction) and early recovery (1133
hospital days vs 1554 hospital days p lt 005) -Results
above are attributed to the anti-inflammatory and anti-
oxidant properties of hydrogen water and reducing
intracellular ROS and facilitation of type-I collagen
construction in dermal fibroblasts and epidermal keratinocytes
Efficacy of Molecular Hydrogen for Skin
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
51
International Journal of Biological Sciences 2013 9(9)887-894
Hydrogen as a New Class of Radioprotectant Liren Qian Jianliang Shen Yunhai Chuai Jianming Cai Link httpbitly1cDAncu
Summary
Ionizing radiation damage is caused by hydroxyl radicals (bullOH) created by radiolysis of H2O bull Molecular hydrogen (H2) has antioxidant activities by
selectively reducing bullOH and peroxynitrite(ONOO-) bull Treating cells with hydrogen before irradiation significantly inhibited radiation induced cell apoptosis (decr ROS and caspase 3 activation) in human intestinal crypt cells as well as human lymphocyte cells bull Increased levels of anti-oxidants superoxide dismutase and glutathione-peroxidase bull Hydrogen significantly reduced the severity of dermatitis
caused by radiation accelerated tissue recovery and reduced the extent of radiation-induced weight loss bull Following brain irradiation the hydrogen water group showed decreased hippocampal damage and decreased levels of 8OHdG and MDA as well as increased levels of superoxide dismutase
Efficacy of Molecular Hydrogen as a Radio-protectant
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Methods of Hydrogen Intake
Hydrogen-Enriched Saline Hydrogen Gas Inhalation
Hydrogen-Rich Water Hydrogen Forming
Capsules
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular Hydrogen Options bull Hydrogen gas infused water is one option but saturation of
water from a gas is limited and it dissipates quickly
bull Oral silica hydride releases large amounts of hydrogen gas
(microbubble size) rapidly that also disperse and escape the
body quickly before the cells can respond to the molecular
hydrogen
bull Hydro-ReGen is a more therapeutically potent option that produces molecular hydrogen in smaller (nanobubble size
(100X smaller than microbubbles)) making this a more water
stable form delivering hydrogen to and within the cells for over
a 24 hour period allowing for higher intracellular
concentrations than other delivery options One capsule of
Hydro-ReGen produces more hydrogen than 2 L of hydrogen infused water
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Ingredients in Hydro-ReGen
bull The Hydro-ReGen proprietary blend
contains free magnesium ions that
produce Molecular Hydrogen when
they react with water in the intestine
bull Activated magnesium and calcium
carbonate ldquocontainrdquo the free
magnesium ions that donate free
electrons to the hydrogen ions in
water in the intestine Hydrogen ions
that take up an electron become
hydrogen atoms and two hydrogen
atoms combine to become stable
molecular hydrogen
bull Selenium is added to enhance the
production of Glutathione Peroxidase
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Hydro-ReGen produces Hydrogen
Nano-Bubbles Our special formula containing a blend of alkaline minerals produces Hydrogen Nano-Bubbles (H2 Nano-Bubbles) when it interacts with water This addresses the two
limitations of Hydrogen-Rich Water
1 H2 Nano-Bubbles do not escape they stay stable in a ldquostabilized formrdquo The H2 Nano-Bubble is so small that the electrical charges around the Nano-Bubble allow it to be stable for an extended period of time This is especially important because H2 needs to be stable until it is inside the body where it
can begin to do its work inside the cells
1 H2 Nano-Bubbles are also so small that Henry‟s Law of Gas solubility does not apply Electrolysis and Gas Saturation can only produce H2 Macro-Bubbles and H2 Micro-Bubbles both of which are subjected to dissipate quickly H2 Nano-Bubbles are so small and so stable that we can have up to
85 ppm of Molecular Hydrogen saturated in the water That‟s up to 5 times more hydrogen Since any excess Molecular Hydrogen dissipates from the body consuming the highest potency of Molecular Hydrogen will be the most effective
H2
H2
H2 H2 H2
e- e-
e- e-
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
Mg2+
H2O H2O
H2O
H2O
H2O
H2O
H2 Macro-Bubble
H2 Micro-Bubble
Possible Structure of H2 Nano-Bubble
H2 Nano-Bubble
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Evidence of Hydrogen Nano-Bubbles
bull Evidence that Hydrogen Nano-Bubbles
are produced by our product was
confirmed using NMR (Nuclear
Magnetic Resonance) analysis
bull The figure to the left indicates a
broader spectrum caused by hydrogen
nano-bubbles The control is the dotted
line and the thick line indicates a
difference in hydrogen nano-bubble
content Notice the thicker line is wider
indicating a chemical shift that is
evidence of nano-bubbles of hydrogen
bull Macro-Bubbles and Micro-Bubbles do
not stay in solution long enough to
cause this shift Therefore the presence
of nano-bubbles is clear
bull Our product not only generates high
concentrations of hydrogen it
generates Nano-Bubble hydrogen that
can deliver optimal results to the user
Source httpbitly1lVT12Y
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Product Safety Data
bull H2 has no toxicity even at high doses
Hydreliox 49 H2 50 Helium and 1
oxygen is used to prevent decompression
sickness during deep technical diving
bull Oral Molecular Hydrogen Product tested on
mice 2gmkg dosing
bull No negative effects observed on clinical
observation or on autopsy
bull This equates to 1400 capsulesday for an
average 70 kg patient
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Specific Clinical Conditions with high oxidative stress
showing good clinical responses (my experience)
bull Chronic Fatigue (watch for detoxification symptoms)
bull Fibromyalgia arthritis and generalised aches and pains
bull PCOS and prostaglandin related period pain
bull Autoimmune conditions seen reduction in hashimotos
Ab titres and ANA titres
bull Improved conversion of thyroxine (T4) to triiodothyronine
(T3)
bull Resistant Dysbiosis and inflammation (Probiotics and anti-
inflammatory therapies not effective)
bull Improved mental focus ADHD (dopamine highly
sensitive to oxidative stress)
bull Athletic enhancement over training syndrome (exercise
burn out)
bull Skin inflammatory conditions (eczema psoriasis)
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Some More General Benefits of Hydro-ReGen
bull Increases energy mental clarity and focus through brain antioxidant effect and improved dopamine functioning
bull Stabilises blood sugar levels improved protein absorption and fat metabolism
bull Helps with polycystic ovarian syndrome (insulin resistance and oxidative stress)
bull Improves circulation
bull Improves gut health and bowel movements
bull Reduces general inflammation and allergic response
bull Improves skin health generally
bull Decreases body odors and flatulence
bull Improves the function and utilisation of minerals
bull Reduces general myalgias
bull Reduces snoring
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Dosage
bull Average dose 1-2 morning and night
bull Very toxic (and obese) patients frac12-1 capsule daily
and increase as tolerated to twice daily dosage
bull Children from 2 years of age frac14 - frac12 capsule daily
mixed in cool liquid
bull Dosage can be up to 3 capsules 3 times daily for
maximal results (esp athletes)
bull Once clinical results have been achieved then
maintenance dose is 1-4 capsules daily in divided
doses depending on oxidative stress load to be
managed
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Common Detox Symptoms
Experienced bull Reaction will be dose and toxic load dependant
usually within first few days but can occassionally
build up over a few weeks Resolved with gut
enterocyte repair liver detoxification and anti-
inflammatory support
bull Bloating or change in bowel habits
bull Lethargy mild weight gain (if impaired liver
detoxification processes)
bull Emotionally labile (teary) mental ldquofuzzinessrdquo
bull Headches and myalgias
bull If these symptoms occur cease capsule for 1-2
days then restart at a lower dose (symptoms
commonly dissipate over 1-2 days)
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Clinical Practicalities
bull Symptom results can be experienced
within days to weeks but benefits
improve over time (months)
bull No contraindications or medication
interactions (but do check patients on
warfarin INR levels frequently)
bull Warn patients of potential usual symptoms of detoxification if toxic and
stress load is high
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Molecular hydrogen‟s take
home unique properties bull Its small size can cross blood brain barrier
bull Selective and synergistic antioxidant
bull Anti-inflammatory
bull Enhancing cell signalling and gene
expression
bull Enhanced energy and mental clarity
bull Synergy with all other supplements
bull Preventive anti-aging and therapeutic
applications
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Some Useful Links For
Further Research bull PUBMED Molecular Hydrogen Research
httpswwwncbinlmnihgovpubmedterm=molecular20h
ydrogen5BTitle5D
bull Medical Gas Research
httpsmedicalgasresearchbiomedcentralcomarticles
bull Molecular Hydrogen Studies
httpwwwmolecularhydrogenstudiescom
bull Molecular Hydrogen Videos with Dr Elen
httpswwwyoutubecomchannelUCxu4lSDndhXW_JINXZYY
BkA
bull Molecular Hydrogen Foundation httpwwwmolecularhydrogenfoundationorg
Thank you for your time
Thank you for your time