PowerPoint Presentation

mRNA track lifetime

RNPassemblynuclearcorralled diffusionperi-nucleardiffusiondirectedtransportleading edgediffusionmRNA on the move:the road to its biological destinyEliscovich et al. JBC minireview mRNA on the move: the road to its biological destiny - SUPPLEMENTAL FIGURE Animated slideThe mRNA lifecycle is regulated by the dynamic association with RNA-binding proteins. mRNAs move from transcription to translation (and degradation) as mRNP complexes. In the nucleus, transcripts are capped, spliced, cleaved, and polyadenylated by RNA-binding proteins. Only properly processed mRNAs are exported. Once in the cytoplasm the mRNA can be subcellular localized to specific sites, translated, and eventually, degraded. This slide shows a simulation of mRNA motility within a fibroblast cell. mRNA tracks represent mRNA movements as a function of time, coded from purple/blue to red. Nuclear mRNAs are subject to corralled diffusion during much of the tortuous path through the nucleus due to chromatin confinement. In motile cells, mRNA is mainly diffusive although occasionally travels along the cytoskeleton (directed transport). mRNAs localized to the leading edge have larger diffusion coefficients on average, while the movement of perinuclear mRNAs is more confined. 1

-actin mRNA (GFP)-tubulin (mCherry)Live cell imaging of endogenous -actin mRNA movement in a mouse embryonic fibroblastEliscovich et al. JBC minireview mRNA on the move: the road to its biological destiny SUPPLEMENTARL FIGURE MovieLive cell imaging of endogenous -actin mRNA (green) movement in a mouse embryonic fibroblast. Microtubules (grey) were fluorescently labeled with -tubulin-mCherry and imaged simultaneously with GFP labeled -actin mRNA. The fibroblasts were derived from the MS2 -actin knock-in mouse, a transgenic line with 24 MS2 stem-loops inserted into the endogenous -actin 3UTR (50). tdMCP-GFP was stably expressed to label the mRNA by high-affinity binding to the stem-loops. Images were taken every 35 ms for 100 frames. The movie is played back at 30 frames/second. Movie has been taken by Zachary B. Katz.2