much faster, easier, and more reliableaz621941.vo.msecnd.net/documents/6f321d03-7d5c-48f... · asms...
TRANSCRIPT
Much Faster, Easier, and More Reliable than Liquid-Liquid Extraction
www.phenomenex.com/Novum
novumsimplified liquid extraction
PATENT PENDING
Phenomenex l WEB: www.phenomenex.com
Simplify Your Liquid-Liquid Extraction Now you can alleviate the challenges associated with traditional liquid-liquid extraction with Novum™ Simplified Liquid Extraction (SLE) (patent pending).
© 2014 Phenomenex, Inc. All rights reserved.
1. Load Your Sample in aqueous solvent
2. Collect Your Target Analytes in water immiscible solvent
Interferences (i.e. phospholipids, proteins, salts, etc.)
Target Analytes
No emulsions
No need to
manually separate liquid
phases
A Simple, Automatable Procedure
Phenomenex l WEB: www.phenomenex.com
0 %
20 %
40 %
60 %
80 %
100 %
120 %
140 %
Cortic
oste
rone
Cortis
one
Cortis
one A
ceta
te
Prednis
one
Testo
stero
ne
Triam
cinolo
ne A
ceto
nide
Rec
over
y (%
)
Lot 1 Lot 2 Lot 3
11α-
OH-pro
geste
rone
A Reliable, Readily Available Product
The unique, synthetic sorbent makes Novum™ SLE a consistent, reliable product in terms of performance and availability.
A consistent, reliable product
proudly manufactured at Phenomenex Headquarters in
Torrance, CA USA
Achieve Consistent Recoveries from Lot-to-Lot
Extraction method1. Load diluted plasma (diluted 1:1 with water) onto Novum SLE MAX 96-well plate, apply vacuum for 2-15 seconds
2. Allow sample to soak into Novum SLE sorbent for 5 minutes
3. Elute with ethyl acetate
Phenomenex l WEB: www.phenomenex.com
Eliminate Interferences from Your AnalysisPhospholipids are present in all bioanalytical samples and can cause a variety of issues in LC/MS/MS analysis including ion suppression, reduced column lifetime, and reduced mass spec sensitivity. Removing phospholipids using Novum™ SLE will dramatically improve your LC/MS/MS data and can increase column lifetime, providing significant cost savings.
Phospholipid Concentration after Cleanup with Novum SLE
Extraction Solvent Lyso 1 Lyso 2 PC 1 PC 2 PC 4Ethyl Acetate (EtOAc) 0 % 0 % 0 % 0 % 0 %
Complete removal of 5 commonly monitored
phospholipids
Lyso 1: 1-Palmitoyl-2-OH-sn-glycero-phosphocholine (m/z 496-184)Lyso 2: 1-Oleoyl-2-OH-sn-glycero-phosphocholine (m/z 522-184)PC 1: 1-Palmitoyl-2-Oleoyl-sn-glycero-phosphocholine (m/z 761-184)PC 2: 1-Stearoyl-2-Lindoleoyl-sn-glycero-phosphocholine (m/z 787-184)PC 4: 1-Oleoyl-2-Lindoleoyl-sn-glycero-phosphocholine (m/z 784-184)
Phospholipids in Diluted Plasma vs. Novum SLE Extracted Plasma
Phospholipid profile monitored using m/z 184-184
00 2 4 6 8 10
100000
22729
90000
80000
70000
60000
50000
40000
30000
20000
10000
App
ID 2
2729
10x Zoom
1/100th Dilution of Precipitated PlasmaNovum EtOAc Extraction
00 2 4 6 8 10
10000
22730
9000
8000
7000
6000
5000
4000
3000
2000
1000
1/100th Dilution of Precipitated PlasmaNovum EtOAc Extraction
00 2 4 6 8 10
10000
22730
9000
8000
7000
6000
5000
4000
3000
2000
1000
App
ID 2
2730
Novum SLE greatly reduces the presence of
phospholipids which will result in improved LC/MS/MS analysis.
Phenomenex l WEB: www.phenomenex.com Phenomenex l WEB: www.phenomenex.com
0 %
20 %
40 %
60 %
80 %
100 %
120 %
Amphe
tam
ine
Bupre
norp
hine
Codein
e
Fent
anyl
MDM
A
MDEA
Mep
eridine
Met
hadon
e
Mep
robam
ate
Met
ham
pheta
mine
Norfe
ntan
yl
Norm
eper
idinePCP
Propox
yphe
ne
Sufen
tanil
Tem
azep
am
Tram
adol
Nordiaz
epam
Rec
over
y (%
)
Consistent, High Recoveries of Target Analytes
Avoid Inferior Results Due to EmulsionsEmulsions are associated with traditional liquid-liquid extraction and are the root cause of analyte loss and contamination. Novum™ SLE eliminates the formation of emulsions, maximizing your analyte recovery while reducing contamination.
Analyte % RSD
Amphetamine 3 %
Buprenorphine 5 %
Codeine 10 %
Fentanyl 6 %
MDEA 4 %
MDMA 4 %
Meperidine 9 %
Meprobamate 7 %
Methadone 2 %
Methamphetamine 12 %
Nordiazepam 1 %
Norfentanyl 3 %
Normeperidine 4 %
PCP 2 %
Propoxyphene 9 %
Sufentanil 11 %
Temazepam 2 %
Tramadol 9 %
Trust Your ResultsNovum SLE simplifies the liquid-liquid extraction process and provides consistent recoveries from sample to sam-ple. Stop worrying about analyte loss due to incomplete manual separation of liquid phases or the formation of emulsions.
Extraction method1. Load diluted urine (diluted 1:1 with 0.5 M Ammonium hydroxide) onto Novum SLE MAX 96-well plate, apply vacuum for 2-15 seconds
2. Allow sample to soak into Novum SLE sorbent for 5 minutes
3. Elute with ethyl acetate
> 80 % recoveries for most analytes
Phenomenex l WEB: www.phenomenex.com
A Simplified Liquid Extraction Novum™ SLE will instantly increase your throughput by eliminating time consuming steps and reducing the risk of analyte loss. If further time savings are necessary, Novum SLE can be easily automated for rapid, hands free sample cleanup.
1. Russell Grant, Matthew Crawford, Brian Rappold, and Stacy Dee. Errors in Bioanalysis Due to Phospholipids – Definitive Measurement, Mechanism, and Management. ASMS 2011.
Visit www.phenomenex.com/Novum for buffer and elution solvent recommendations, technical notes, demonstration videos, and more!
Novum Simplified Liquid Extraction (SLE)
Estimated Time per Sample = <15 minutes
2. Wait 5 minutes
1. Dilute sample 1:1 with buffer or water and load onto Novum SLE sorbent using 2–15 seconds of vacuum
3. Apply elution solvent and allow to elute via gravity. Complete elution with 10 seconds of vacuum.
• Rapid, automatable method for high throughput cleanup
• Stop worrying about analyte loss due to emulsions
Fast and Easy
Traditional Liquid-Liquid Extraction1
Estimated Time per Sample = 25 minutes
4. Pour off or freeze supernatant
1. Dilute sample 1:1 with buffer or water and add extraction solvent
2. Mix for 10 minutes
24 23 22 21 20 19 18 17 16 15 14 13 12 11 10 9 8 7
6
5
4
3
2 1
3. Centrifuge for 10 minutes
Slow and Laborious
Phenomenex l WEB: www.phenomenex.com Phenomenex l WEB: www.phenomenex.com
Simplify Your Liquid-Liquid Extraction NowOrdering Information
Get Answers to Your QuestionsYour Sample Preparation Specialist is available to assist with method development and optimization. Ask us how Novum SLE can improve your analysis. www.phenomenex.com/mysupport
Information at Your FingertipsAccess applications, videos, webinars, and more! www.phenomenex.com/Novum
Terms and Conditions Subject to Phenomenex Standard Terms and Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions. Trademarks Novum is a trademark of Phenomenex. Disclaimer Novum is patent pending.
© 2014 Phenomenex, Inc. All rights reserved.
If Novum SLE products do not perform as well or better than your current SLE product, return the product with comparative data within 45 days for a FULL REFUND.
AccessoriesCollection Plates (deep well, polypropylene)Part No. Description Unit PriceAH0-7192 96-Well Collection Plate, 350 µL/well 50/pkAH0-7193 96-Well Collection Plate, 1 mL/well 50/pkAH0-7194 96-Well Collection Plate, 2 mL/well 50/pkAH0-8635 96-Well Collection Plate, 2 mL Square/Round-Conical 50/pkAH0-8636 96-Well Collection Plate, 2 mL Round/Round, 8 mm 50/pkAH0-7279 96-Well Collection Plate, 1 mL/well Round, 7 mm 50/pkSealing MatsAH0-8597 Sealing Mats, Pierceable, 96-Square Well, Silicone 50/pkAH0-8598 Sealing Mats, Pre-Slit, 96-Square Well, Silicone 50/pkAH0-8631 Sealing Mats, Pierceable, 96-Round Well 7 mm, Silicone 50/pkAH0-8632 Sealing Mats, Pre-Slit, 96-Round Well 7 mm, Silicone 50/pkAH0-8633 Sealing Mats, Pierceable, 96-Round Well 8 mm, Silicone 50/pkAH0-8634 Sealing Mats, Pre-Slit, 96-Round Well 8 mm, Silicone 50/pkAH0-7362 Sealing Tape Pad 10/pk
Vacuum ManifoldAH0-8950 96-Well Plate Manifold, Universal with Vacuum Gauge ea
Novum™ Simplified Liquid Extraction (SLE)Part No. Description Unit Price8E-S138-FGA Novum SLE MINI 96-Well Plate 1/Box8E-S138-5GA Novum SLE MAX 96-Well Plate 1/Box
1. Russell Grant, Matthew Crawford, Brian Rappold, and Stacy Dee. Errors in Bioanalysis Due to Phospholipids – Definitive Measurement, Mechanism, and Management. ASMS 2011.
Much Faster, Easier, and More Reliable than Liquid-Liquid Extraction
www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at [email protected]
novumsimplified liquid extraction
Australiat: +61 (0)2-9428-6444 f: +61 (0)2-9428-6445
Austriat: +43 (0)1-319-1301f: +43 (0)1-319-1300
Belgiumt: +32 (0)2 503 4015 (French)t: +32 (0)2 511 8666 (Dutch)f: +31 (0)30-2383749
Canadat: +1 (800) 543-3681f: +1 (310) 328-7768
Chinat: +86 (0)20 2282-6668f: +86 (0)20 2809-8130
Denmarkt: +45 4824 8048f: +45 4810 6265
Finlandt: +358 (0)9 4789 0063f: +45 4810 6265
Francet: +33 (0)1 30 09 21 10 f: +33 (0)1 30 09 21 11
Germanyt: +49 (0)6021-58830-0f: +49 (0)6021-58830-11
Indiat: +91 (0)40-3012 2400f: +91 (0)40-3012 2411
Irelandt: +353 (0)1 247 5405f: +44 1625-501796
Italyt: +39 051 6327511f: +39 051 6327555
Luxembourgt: +31 (0)30-2418700 f: +31 (0)30-2383749
Mexicot: 001-800-844-5226f: 001-310-328-7768
The Netherlandst: +31 (0)30-2418700 f: +31 (0)30-2383749
New Zealandt: +64 (0)9-4780951f: +64 (0)9-4780952
Norwayt: +47 810 02 005f: +45 4810 6265
Puerto Ricot: +1 (800) 541-HPLCf: +1 (310) 328-7768
Spaint: +34 91-413-8613f: +34 91-413-2290
Swedent: +46 (0)8 611 6950f: +45 4810 6265
United Kingdomt: +44 (0)1625-501367f: +44 (0)1625-501796
USAt: +1 (310) 212-0555f: +1 (310) 328-7768
All other countries Corporate Office USA
t: +1 (310) 212-0555f: +1 (310) 328-7768
BR
2438
0714
_W