neha agrawal 18.10.16
TRANSCRIPT
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Scale of Fermentation process:- Large Scale, Small
Scale & Pilot Scale Fermentation
Presented by:Neha Agrawalm.sc. 3rd sem
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CONTENT• Introduction
• Component of fermenter• Scale of Fermentation
–Small scale fermentation • Objectives•Material and method• Fermentation design
–Large scale fermentation–Pilot scale fermentation• Advantages & disadvantages of fermenter
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Ranges of fermentation processTo produce microbial cells or biomass
To produce microbial enzymes.
To produce microbial metabolites.
To produce recombinant products.
To modify a compound which is added to the fermentation ( transformation)
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FERMENTER
• A fermenter is basically a device in which the organism are cultivated to form the desired product.
• It is containment system design to give right environment for optimal growth and metabolic activity of the organism
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SCALE UP OF FERMENTER
Small scale fermentor
Large scale fermentor
Pilot scale fermentor
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SMALL SCALE FERMENTER • Small scale fermenter are same as large fermenter (bioreactor).
• They are two type : bench scale or lab scale system.
• They are small but highly automated and customized for the application.
• Precursor of large plant.
• They can lab as CSTR or autoclave fixed, fixed on fluidized bed reactor application.
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OBJECTIVE• Minimize manual handling.
• Releasing economic on scale.
• Production if biopharmaceutical.
• Large scale experimentation are time consuming and expensive.
• They are use to exphase key variable before a move to large scale bioreactor is made.
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MATERIAL AND METHOD
• Tank- bioreactor• Media- media for animal (serum, serum free) for plant (MS, B5) for microbes (urea enrichment)• Power requirement-• Inoculums- Inoculation of small scale fermenter of
250-1000 liter capacity. Fermenter inoculating using submerged inoculums for
seed fermenters.
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• Maintains and optimum condition- pH Temperature Agitation Aeration oxygen demand• Harvesting – Product are two type- Intracellular (Biomass) Extracellular (Remove cell)
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• If the product are extracellular, product is removal of large solid particle and microbes cell usually be centrifugation.
• The broth is fractionated or extracted in to major fraction using ultracentrifugation, reverse osmosis, gel filtration/ ion exchange/ adsorption.
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FERMENTER DESIGN
• This largely determines the ranges of temperature and pH that can considered, the degree of aerobiocity required and the probable effect of contamination.
• The selection of fermenter configuration, i.e. a form of batch stirred, continuous stirred tank or tubular fermenter.
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• The determination of fermenter dimension, e.g. volume and diameter, and the values of the operating variables, temp., and pH .
• The extent of the heat transfer surface and the mixing devise required.
• Power and aeration requirements.
• The mechanical design, including selection of materials of construction and maintenance of aseptic conditions.
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SMALL SCALE FERMENTATION DESIGN
• Two type of fermenter- Bench fermenter Lab scale fermenter.
Capacity-(1 – 100 liter). Height Ratio 2:1. Portable and fixed.
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LARGE SCALE FERMENTER
• They are large capacity is variable.
• Many size variable.
• 2:1 ratio is common.
• Common type CSTR.
• E.g.:- bioreactor.
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PILOT SCALE FERMENTER
A pilot scale fermenter is a small industrial system, which is operated to generate information about the behavior of the system for use in design of larger facilities.
Pilot plant is a relative term in the sense that plants are typically smaller than full-scale production plants, but are built in a range of sizes.
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