gus

gus

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(Gossypium hirsutum L.)

LBA4404pBI121gusnptII

CaMV35SNOSgus

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(T1)

gusA3A4A7

(T1)nptII

(T1)PCRgus

gus

*

E-mail:gtohidfar@yahoo.com

(

uid

Gh-rbcsGh-spgus

Gh-spGh-rbcs

pBI121gusnptII

gus

gus

GhTUB1

GhTUB1

gus

LBA4404pBI121gus

CaMV35SnptII

Nos

OD600

mg/1

gus

MSKNO3

MgCl2

B5

MSB5

MS

GUS

(PCR)

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gusnptIIOligo

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GUS 3'-ACGTCCTGTAGAGAAACCCCAA-5

GUS 5'-CCCGCTTCGAAACCAATGCC-3

NPTII 3'-GAACAAGATGGATTGCACGC-5

NPTII 5'-GAAGAACTCGTCAAGAAGGC-3

TaqDNA

1. Cefotaxime

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MgCl2

X

PCR

gus

T0

(T1)

MS

pH=8

GUS

2

nptII

nptII

PCR

nptII

nptII

gus

gus

gus

gus

PCR

nptII

DNA

DNApBI121

DNA

DNA

gus

gus

GUS

gus

PCR

gus

gus

PCR

gus

DNA

DNA

DNA

DNApBI121

DNA

gus(T1)

T0gus

gus

gus

gus

gus

GUS+ GUS-

A1 44 A2 4 3 A3 52 A4 6 2 A5 8 0 A6 4 4 A7 5 2 A8 5 3

gus

A3A4A7gus

A1A6

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GUS

gus nptII

gus

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DNA

DNA

DNA

DNApBI121

DNADNA

DNA

gus

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LBA4404EHA105

EHA105LBA4404

LBA4404

EHA101EHA105

REFERENCES1. Banerjee, A. K., D.C. Agrawal, S.M. Nalawade & K.V.Krishnamurthy.2002. Transient expression of

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2. Banerjee, A.K., D.C. Agrawal, S.M. Nalawade, S. Hazra, & K.V. Krishnamurthy. 2003. Multiple shoot induction and plant regeneration from embryo axes of six cultivars of Gossypium hirsitum. Biologia Plantarum 47(3):433-436

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gus

21. Song, P.H., J. L. Heinen, T.H. Burns & R. D. Allen. 2000. Expression of two tissue-specific

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