obtaining ions, nutrients and water. semipermeable membranes regulate cell interaction with...
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Obtaining Ions, Nutrients and Water
semipermeable membranes regulate cell interaction with surroundings
→small openings (pores) allow certain molecules to penetrate cell but denies others making it semi
Small black molecules allowed through
Small black molecules allowed through
Large red molecules NOT allowed through
Large red molecules NOT allowed through
WHY?WHY?
o Plasma membrane is composed of a phospholipid bilayer (two layers of phospholipids or lipids with phosphates)
2 layers = 1 plasma membrane
2 layers = 1 plasma membrane
Phosphate head
Lipid tails
• phosphate group: phosphate (PO4)
>Polar / hydrophilic (water-loving) globular heads, form outside of membrane (can be exterior or interior side of cell)
>Can dissolve in water, allows plasma membrane of cell to interact with watery environment of cell.
Phosphate head
Lipid tail
• properties of phospholipids do NOT allow water-soluble molecules to move through membrane easily.
H 2O
solu
ble
H 2O
solu
ble
o Proteins embedded within membrane move about in a fluid fashion♦ Fluid mosaic model explains how structural
properties of plasma membrane allow lipids & proteins to move freely in membrane
Mosaic: something consisting of variety of components
o Plasma membrane also contains:♦ Transport proteins: allow substances or waste
materials to move through plasma membrane♦ Proteins & carbohydrates: stick out from cell and
help cells identify each other–Proteins: give cell flexibility–Carbohydrates: act as identifier of cell type
Cell constantly interacts with environment & has to respond to external conditions to maintain homeostasis
98.6ºF98.8ºF99.0ºF
Increase in heat makes temperature
rise
Increase in heat makes temperature
riseCell will seek to cool
itself downCell will seek to cool
itself down
Homeostasis at work!Homeostasis at work!
Eating a bag of chips will cause cell to respond to new salty
condition
Cell membranes maintain homeostasis by regulating passage of materials into and out of the cell:• Passive Transport – no energy required to
move solute or solvent from high to low concentration (down concentration gradient)
• Active Transport – energy required to move solute from low to high concentration (against concentration gradient)
Outside Cell (16) Inside Cell (4)
outside
concentration gradient = difference in amount of substance present inside the cell versus outside the cell
inside
→Molecules will freely flow from high concentration to low concentration
→Pathway from high to low concentration is concentration gradient
→Eventual point where concentration is equal = equilibrium (particles still randomly move but equal in/out)
Concentration Gradient
High Concentration
Low Concentration
Three methods:Three methods:a.a. Simple diffusion Simple diffusion – particles – particles
(solutes)(solutes)b.b. OsmosisOsmosis – diffusion of water – diffusion of water
(solvent)(solvent)c.c. Carrier-facilitated diffusionCarrier-facilitated diffusion – –
particles (solutes) with protein particles (solutes) with protein assistanceassistance
Simple diffusion – free movement of ions & molecules through protein channels in membrane
Outside cell
Inside cell
Simple Diffusion – the homemade animation
o Osmosis – free movement or diffusion of solvent (water) ONLY
Note how the red sugar particles stay put. Only the blue water molecules move.
Osmosis– the homemade animation
o three types of solutions that determine direction of water :♦ isotonic solution♦hypotonic solution♦hypertonic solution
♦ Isotonic Solution> concentration of water on outside of cell is same as on inside of cell>Cells do not experience overall osmosis (or
diffusion)>retain their normal shape,>Still random movement for an overall net gain of 0
90% H90% H22OO10% 10%
particlesparticles(inside (inside
cell)cell)90% H90% H22OO
10% 10% particlesparticles(outside (outside
cell)cell)
♦ Hypotonic Solution> concentration of water is higher outside cell
than concentration of water inside cell.>Cells experience osmosis (and diffusion)
>water moves into cell >cell swells and will burst if animal cell but will not burst if plant cell due to cell wall
30% H30% H22OO70% 70%
particlesparticles(inside (inside
cell)cell) 70% H70% H22OO30% 30%
particlesparticles(outside (outside
cell)cell)
Osmosis of waterDiffusion of particles
♦ Hypertonic Solution> concentration of water is higher inside cell
than concentration outside cell.>Cells experience osmosis (and diffusion)> water moves out of cell >Cells shrink
>Plant wilts because of a decrease in pressure on cell wall
55% H55% H22OO45% 45%
particlesparticles(inside (inside
cell)cell)45% H45% H22OO
55% 55% particlesparticles(outside (outside
cell)cell)
Osmosis of water
Diffusion of particles
Hypertonic Isotonic Hypotonic
Higher solute OUTSIDE
Equal solute Higher solute INSIDE
Higher water INSIDE
Equal water Higher water OUTSIDE
Water moves OUT
No net movement
Water moves IN
Cell SHRINKS Normal Cell SWELLS
Hypertonic vs. Isotonic vs. Hypotonic Solutions
Hypertonic vs. Isotonic vs. Hypotonic Solutions
Hypertonic vs. Isotonic vs. Hypotonic Solutions
Often times, diffusion of large particles can’t happen or happen fast enough
Water will diffuse via osmosis to regulate concentration (PERCENTAGES)•Outside cell is 50% solute, 50% water• Inside cell is 10% solute, 90% water•Solute is too big to move. Will we die? No.
Water will move to get concentration equal
90% H90% H22OO10% 10%
particlesparticles(inside (inside
cell)cell)
50% H50% H22OO50% 50%
particlesparticles(outside (outside
cell)cell)
Hint: look at water % only. Where will the water go?
20% H20% H22OO80% 80%
particlesparticles(inside (inside
cell)cell) 80% H80% H22OO20% 20%
particlesparticles(outside (outside
cell)cell)
Diffusion of particles
Osmosis of water
Question1: Which way will the particles move?
Question 2: Which way will the water move?outside
inside
OSMOSIS
DIFFUSION
free movement of larger molecules with aid (help) of a transport/carrier protein• Large solutes (sugars, amino acids) are too big to
go through plasma membrane unaided
Facilitated Diffusion – the homemade animation
Needs energy (ATP) to move particles across plasma membrane• Three types:1. Active transport – small molecules AGAINST
concentration gradient (from low to high)2. Endocytosis – large molecules being engulfed
by plasma membrane into vesicles3. Exocytosis – large molecules being expelled
out by vesicles out of plasma membrane
Requires energy (ATP) to move materials against gradient (low to high concentration)
♦ carrier protein allows specific molecule or ion to bind
o Example: sodium/potassium (Na/K) pump♦ATP changes shape of protein to move molecule or ion across plasma membrane
ATP
cell surrounds material with a portion of its plasma membrane and forms a vesicle
Vesicle moves particle inside cell for digestion•Bacteria, foodPhagocytosis – large, solid particles
Pinocytosis – small, liquid particles
STARRINGSTARRING
train as the CELLtrain as the CELL
platform as the platform as the EXTRACELLULAR SPACEEXTRACELLULAR SPACE
people as the people as the PARTICLESPARTICLES
conductors as the ATPconductors as the ATP
Vesicle brings material to cell membrane & expels (secretes) it •Wastes, hormones
Test Wednesday:40 points multiple choice10 points short answer