RJ Almeida, DSS Meireles*, ACB Filho, MC Pereira, AC Lima, ACA Silva, AM Loyola, NO Dantas, CJA Sousa.

drronaldoalmeida@hotmail.com

65

Thus, through the methodology used was possible to evidence the osseointegration phenomenal and biocompatibility of an aloplasthic material containing nanocrystals of titanium dioxide. We can also conclude that there was direct bone deposition on the oxide, what can be called osteointegration. The material was gotten through the synthesis of a nano powder using the

3humid one way precipitation, Pechini method on ambient temperature (Fig. 1). After temperature treatment was structurally characterized by X-ray Diffraction (XRD) (Fig. 2a), Confirming titanium dioxide synthesis on Rutilo and Anatasio phases, with tetragonal crystalline symmetry and medium size of 21 nm (Fig. 3). We characterize using de Raman micro spectroscopy too. (Fig. 2b) The vibration asset ways typical on TiO nanocrystals on Rutilo e Anatase phases, confirming the pureness on the 2

synthesis through the absence of others nanostructures identified by Raman specter (Fig. 4). After that, the biocompatibility test was made using intra bone implantation of the TiO powder inside a teflon cup in guinea pigs jaw. (Fig. 5) Previously 2

approved by the CEUA/UFU 101/10. Ten guinea pigs were used on the experimental periods: 4 and 12 weeks. Each animal received a Teflon cup containing TiO nanocrystals. From each implant was made 24 blades with 144 cuts colored by 2

hematoxilin-eosine method and analyzed by two independent observers by means of inflammatory intensity response scores, considering all Teflon cup board. The analysis was criteriously interpreted following the FDI normative as acceptable or not acceptable to be related to bone.

Syntetase titanium dioxide (TiO ) nanocrystals on high degree of pureness; Evaluate its biocompatibility following 2

1 2parameters defined and accepted for the Dental International Federation ( FDI) and American Dental Association (ADA):

Evidence its nanotopograph in the interface of the new bone through Atomic Microscopy Force (AMF)

It has been estimated that a 500 thousand bone craft procedures is made each year around the world. However little success is being reached in procedures using aloplastic materials, essentially because its osseointegrate incapacity.

Figura 1

Figura 2 Figura 3 Figura 4 Figura 5

BONE ACCESSION TO TIO2 NANOCRYSTALS: EVIDENCE OF BIOCOMPATIBILITY AND OSSEOUS INTEGRATION

Fig 6: (a) General vision, (b) giant cells delicate layer, (c) osteóide material, (d) Osteocite presence and TiO2 attached to bone.

Figure 7: (a) General vision, (b) appalling of the material, (c) material on gaps and bone and lamellar bone formation, (d) direct bone deposition.

Thus, through the methodology used it was possible to demonstrate the

phenomenon of osseointegration and biocompatibility,at the nanoscale, of

na alloplastic material containing titanium dioxide nanocrystals. We also

conclude that there is direct bone deposition on the oxide, which could be

called oxideintegration.

The Foundation for Research Support of the Minas Gerais State- FAPEMIG-

for financial support through the process APQ-No. 02561-10

1-Technical Report no 9 F.D.I. Langeland,K; Cotton,W.R. Recommended

standard practices for biological evaluation of dental materials. International

Dentistry Journal, 1980; 30(2): 140-88.

2-American Dental Association-American National Standards Institute.

Document No 41 for recommend standard practices for biologic evaluation of

dental materials. Council of Dental Materials and Devices, 1982.

3-Pecchini,M.P; Adams,N. Method of preparing lead and alkaline earth

titanates and niobates and coating method using tha same to form a

capacitor. United States Patent Office, 1967.

Histologycal Parameters Number of Implants TiO NanoCristals Control2

4 weeks 12 weeks 4 weeks 12 weeksNeutrophils Macrophages Linphocytes Plasma cells Giant cells Scatterd material Capsule Remodeling bone tissue Necrotic bone tissue Reabsorption

Inflammatory reaction --------------------------------------------------------------------------------------------------Absent ( +) ; Moderated (++); Severe (+++)

++++

+ + + ++

+ + +++

+

++++

+ + + ++

+ + +++

+

+++++ +++++

+

+++++ +++++

+

Material Experimental Periods (weeks) Number of Implants

2 TiO

Control

30

90

30

90

10

10

10

10

Table 01 =Experimental time and implant number

Table 02 = Evaluation of inflammatory reaction after 04 weeks and 12 weeks.