packed column gc application guide

Packed Column GCApplication Guide

1

Bulletin 890A

Packed Column GCApplication Guide

This guide to packed column applications provides information on thebest approach to designing your application. It includes helpfulinformation about packings, phases, tubing, and different types ofdeactivations. Nearly 200 different applications are organized ac-cording to type of analysis. In most cases, the catalog number for thepacking is included with the application chromatogram.

Key Wordsl packed column l gas chromatography

What Makes a Good Column?Tubing, support, and stationary phase are all that is needed to prepare acolumn, but to prepare a column that will provide the desired separation mayrequire much more. First, the proper materials must be selected; this can bethe most difficult part. Also, these ingredients must be combined in such amanner that optimum performance can be obtained; this can turn out to bemore than one bargains for! Any compromise in materials or procedures yieldsa compromised performance.

The Rohrschneider and McReynolds ConstantsAn important feature is the use of the Rohrschneider and McReynoldsConstants. Using these systems, most of the stationary phases now in use havebeen characterized by a series of constants. These constants provide theworker with a wealth of practical information to help solve problems in columnselection. For more information and a listing of McReynolds values, requestBulletin 880.

The SupportThe support plays a critical role in several ways in the performance of thecolumn. First, it governs the efficiency of the column (narrowness of peaks).The structure of the support, and the manner in which it is coated, bothcontribute to the efficiency. Secondly, the wrong choice of support caninteract with the sample to cause the chromatographic peaks to tail, i.e., theycan be highly asymmetrical and consequently difficult or impossible tomeasure. Ideally, the support should not interact with the sample but, inpractice, this does occur. By careful selection of the support and conditions,one can minimize this problem.The tailing phenomenon is caused by active sites on the surface of the support.These sites are ones that can form a hydrogen bond; consequently samplesthat form a strong hydrogen bond tail badly. In practice, compounds such aswater, glycols, alcohols, acids, and amines tail severely while carbonylT195890A©1999 Sigma-Aldrich Co.

2

compounds such as esters, ketones, and aldehydes tail to a lesser degree.Hydrocarbons that do not hydrogen bond are not bothered by tailing.To eliminate or reduce the tailing problem one modifies the support surfaceby: 1) removing the active sites by acid and/or base washing, 2) modifying thesurface by silanization, or 3) covering the active sites with the stationary phasehaving polar functional groups in it.Acid washing is effective in removing mineral impurities from the supportsurface as well as miscellaneous extraneous material. Base washing does notimpart any special advantage to the support that is not obtained with a wellacid washed support. Acid washing by itself is not effective in reducing tailingbut it is recommended where a polar phase is used, such as the polyesters andpolyglycols.Silanization, particularly with dimethyldichlorosilane (DMCS) is very effectivein reducing tailing. Combined with acid washing and DMCS treatment, theresulting support is recommended for most columns. Silane treatment is avery difficult process to control and should not be attempted unless you wishto make a research project out of it. When silicone stationary phases are used,it is mandatory that an acid-washed and DMCS-treated support be used. Thesilicone stationary phases, particularly when used in the 1-5% level, are noteffective in deactivating the support and require a silane-treated support.SUPELCOPORT is recommended where an acid-washed and DMCS-treatedsupport is required.The third procedure for deactivation, using a polar stationary phase, requiresthat the phase contain functional groups such as an ester, an ether, a hydroxyl,and an amine group. These functional groups have strong hydrogen bondingcharacteristics and tie up the active sites on the support surface. These phasesdo not require a silanized support, although, when they are used at a level of5% or less, silanization can be useful. Normally we recommend using an acid-washed support with these phases.When analyzing acids it is necessary that the stationary phase contain an acidto deactivate the support. See Bulletin 856.When working with basic compounds such as amines, the stationary phasemust contain a base to deactivate the support; otherwise severe tailing willresult. KOH is frequently used at a 1-2% level for the purpose. A basicstationary phase such as polyethyleneimines also may be used.Most of the GC supports in current use are made from diatomaceous earth,also called diatomite. The diatomite is processed in several ways producingtwo basic types of supports. These are conveniently recognized by their color.The particle size of supports are generally expressed in terms of screenopenings since screens are normally used to prepare them. The particle sizesnormally used in GC are as follows:

60/80 mesh 250-177 microns80/100 mesh 177-149 microns100/120 mesh 149-125 microns

The designation 60/80 mesh means that the particles have passed through a60 mesh screen (-60) and will not pass through the 80 mesh screen (+80). Itthen means that the particles are between 250-177 microns in size. Thecolumn efficiency improves with decreasing particle size. At present the80/100 mesh is the most popular size, but 100/120 mesh is used withincreasing frequency when more efficiency columns are desired.

3

Column TubingGlass, stainless steel, aluminum, and copper are the tubing materials com-monly used for columns. While glass is the most inert of the tubing, stainlesssteel is the most widely used.Glass is used in situations where the sample might interact with the walls ofthe tube. It is standard operating procedure to use glass columns whenworking with pesticides and biochemicals such as steroids and hormones.Glass is more inert then the metals and rarely causes tailing or decompositionof the samples. Glass columns are also used generally in situations where it isdesirable that the sample be injected directly into the column. An alternativeis to use a glass lined injection port to avoid the contact with metal since metalin the inlet can destroy certain samples or cause tailing. Glass columns alsopermit you to see how well a column has been packed.Fused silica lined stainless steel tubing is a suitable substitute for glass tubing.When properly deactivated, it meets or exceeds the deactivation of conven-tional deactivated glass columns.Metal columns are used where glass is not required. Stainless steel is generallyconsidered more inert than aluminum or copper. We recommend stainlesssteel as the metal of choice because it is easier to prepare columns of higherefficiency with stainless steel than with aluminum or copper. The hardness ofthe material appears to be important in transmitting the vibrational energywhen the column is vibrated or tapped. However, if in doubt . . . use glass. . . and silane treat it! (All Supelco glass columns are silane-treated.)SP-Alloy T-1, our nickel alloy, approaches glass in inertness. Most newerinstruments are designed to handle 1/8" OD metal columns. When glasscolumns are to be used, the instruments are usually equipped to handle 1/4"OD columns. Here the glass column can be made with a heavy glass wallcutting down considerably on the problem of breakage. Where 1/8" OD glasscolumns are used, the breakage problems are usually severe.From our own experience we recommend using 2mm ID rather than 4mm IDglass columns because of the inherently higher efficiency. If you shift from4mm to 2mm columns, remember to reduce the carrier gas flow rate to 1/4,not 1/2. A reasonable flow rate for 4mm columns is 80mL/min, while for 2mmcolumns a good rate is 20mL/min.

Upper Temperature LimitEach stationary phase has an upper temperature limit above which the columnshould not be operated. Most stationary phases are polymers that consist ofmaterials having a range of molecular weights. As the column temperatureis increased, the more volatile portion of the polymer is swept out of thecolumn by the carrier gas. The volatile products could also be formed bythermal degradation of the stationary phase while the column is being used.This is called bleed, and is seen on the recorder as a rise in the baseline or asnoise. Above the maximum upper limit the bleed rate is very high and thecolumn will have a relatively short life. In some cases the bleed rate is so highthat it will not be possible to move the recorder pen off of full scale. Note thatthe reported maximum upper limits are approximate values. Each columnproduced is shipped with a conditioning sheet, and minimum/maximumoperating temperature is indicated. For a listing of phases and their maxi-mum/minimum operating temperatures, refer to our general catalog.

4

Conditioning the ColumnBefore a column is used for the first time, it must be conditioned for a periodof hours to rid it of the very volatile portion of the stationary phase and alsothe last traces of solvent which were used in the coating step.We recommend conditioning the column at the maximum isothermal columntemperature which you expect to reach. One can start to use a column after ithas been conditioned at that temperature after only 3-4 hours, but condition-ing overnight is usually better. We recommend that you always condition thecolumn with a flow of carrier gas. Others have recommended “no-flow’conditioning for such materials as UC W98 silicone. This might be necessaryfor technical grade silicone, but chromatographic grade silicones such as theSPTM and OV® phases, and most other phases, should not be subjected to thisdrastic treatment.Many stationary phases are subject to oxidation and should not be exposedto air while hot. At all times when the column is hot, the carrier gas shouldbe allowed to flow through it. Cutting back on the carrier gas overnight canbe detrimental to the column. It may also be necessary to insert an oxygenscrubber in the carrier gas line.

The Lower Temperature LimitObserving the lower temperature limit is also important to obtain goodcolumn performance. Some stationary phases are solids and are ineffective asa stationary phase in this form. They must be operated at a temperature abovetheir melting point.

Selecting a ColumnThe simplest method of obtaining a column to handle a particular problem isto duplicate one that is referred to in the applications pages of this guide.Another method is to use the literature as a guide and then to substitute someof the newer, improved stationary phases and solid supports. If you cannotfind the solution to your separation problem in the literature, the problem ismore complex. When ordering the column, it is essential that you list all of thefollowing:a. the stationary phaseb. the supportc. the mesh range of the supportd. the treatment given the supporte. the percent coating of stationary phasef. the tube composition, i.e., glass, stainless, etc.

g. the dimensions of the tube, i.e., length and diameter, both OD and ID ifpossible

h. the configuration of the column or instrument it is to be used withExample: 3% SP-2100 on 100/120 mesh SUPELCOPORTTM,

6' x 1/4" OD, 2mm ID, Glass for HP6890.

For a complete listing of our current line of columns, packings,and accessories, please refer to our general catalog.

5

Index to Chromatograms

Compound Class PageAcetaldehyde ...................................................................................... 6Acids .................................................................................................. 6Acids, Methyl Esters (also see Fatty Acid Methyl Esters) ...................... 11Alcohols ............................................................................................ 13Amines .............................................................................................. 19Amino Acids ...................................................................................... 21Aroclors ............................................................................................. 22Ammonia .......................................................................................... 24Aromatics .......................................................................................... 24Chlorinated Hydrocarbons ................................................................. 28Creosote ............................................................................................ 31Drugs ................................................................................................ 33EPA Methods (600 Series) .................................................................. 37Ethylene Oxide and Residues ............................................................. 46Fatty Acid Methyl Esters ..................................................................... 47Fragrances ......................................................................................... 51Freons ............................................................................................... 53Formaldehyde ................................................................................... 54Hydrocarbons

C1-C2 Hydrocarbons & Permanent Gases ................................ 55C1-C3 Hydrocarbons ................................................................ 59C1-C4 Hydrocarbons ................................................................ 60C1-C5 Hydrocarbons ................................................................ 60C1-C6 Hydrocarbons ................................................................ 62

Permanent Gases and Hydrocarbons.................................................. 64Pesticides .......................................................................................... 68Phenols ............................................................................................. 69Pheromones ...................................................................................... 71Simulated Distillation ......................................................................... 72Solvents ............................................................................................ 78Sugars ............................................................................................... 84Sulfur and Mercaptans ....................................................................... 85Steroids ............................................................................................. 92Sterols ............................................................................................... 94Triglycerides ...................................................................................... 95

Index to

Chro

mato

gra

ms

TrademarksAroclor — Monsanto Co.Bentone — National Lead Co., Baroid Sales Div.Carbopack — Sigma-Aldrich Co.Carbotrap — Sigma-Aldrich Co.Carbowax — Union Carbide Corp.Carboxen* — Sigma-Aldrich Co.Cellosolve — Union Carbide Corp.Chromosorb – Celite Corp.Dexsil — Dexsil Chemical Corp.Fluorcol — Sigma-Aldrich Co.Freon — E.I. du Pont de Nemours & Co., Inc.Hall — Tracor Instruments, Austin, Inc.HayeSep — Hayes Separations Inc.

ORBO — Sigma-Aldrich Co.Oronite — Uniroyal, Inc.OV – Ohio Valley Specialty Chemical CompanyPetrocol — Sigma-Aldrich Co.Qualmix — Sigma-Aldrich Co.Scott — Scott Specialty Gases, Inc.SP – Sigma-Aldrich Co.SUPELCOPORT – Sigma-Aldrich Co.Supelpak — Sigma-Aldrich Co.Teflon — E.I. du Pont de Nemours & Co., Inc.TightSpec — Sigma-Aldrich Co.UCON – Union Carbide CorporationValco — Valco Instruments Co., Inc.*US Pat. No. 4,839,331.

6

795-0077

795-0144

Figure 1. Trace Acetaldehyde in Air (2500ppm)Packing: Hayesep ® D, 100/120 meshCat. No.: 10293 (packing)Column: 3' x 1/8" ID stainless steel

Oven: 100°CCarrier: helium, 30cc/min

Det.: P.E. 900 TC, 225 ma, 140°CInj.: Valco® valve, 100mL, 140°C

Figure 2. Volatile Fatty Acids (C1-C7)Packing: 10% SP-1000/1% H3PO4 on 100/120 Chromosorb ® W AWCat. No.: 11841 (packing)Column: 6' x 4mm ID glass

Oven: Fisher Model 2400, 147°CCarrier: helium, 86mL/min

Det.: TCInj.: 14µL

0 42 6 8 10 14 16 22 24

1

3

4

5

6

7

8 9

10

Min

For more information, request Bulletin 856.

1. Acetic acid2. Formic acid3. Propionic acid4. Isobutyric acid5. n-Butyric acid6. Isovaleric acid7. n-Valeric acid8. Isocaproic acid9. n-Caproic acid

10. Heptanoic acid

2

12

For more information, request Bulletins 790 and 816.Chromatogram provided courtesy of Hayes Separations, Inc.

20Min

3

2

1

1. Air2. Water3. Acetaldehyde (vol. 2500ppm)

7

795-0076

795-0078


1. Formic acid2. Acetic acid3. Propionic acid4. Isobutyric acid5. n-Butyric acid6. Isovaleric acid7. n-Valeric acid8. Isocaproic acid9. n-Caproic acid

10. Heptanoic acid

Figure 3. Volatile Acid Standard Mix (C1-C7)Packing: 15% SP-1220/1% H3PO4 on 100/120 Chromosorb W AWCat. No.: 12144 (packing)Column: 6' x 4mm ID glass

Oven: Fisher Model 2400, 145°CCarrier: helium, 70mL/min

Det.: TCInj.: 14µL

Figure 4. Volatile Fatty Acids in Sewage Effluents(ppm Levels)

Packing: GP 60/80 Carbopack ® C/0.3% Carbowax ® 20M/0.1% H3PO4Cat. No.: 11825-U (packing,15g/bottle)Column: 30" x 4mm ID glass

Oven: 120°CCarrier: nitrogen, 50mL/min

Det.: FID, 200°CInj.: 1µL water containing 50ppm

each analyte, 200°C

Aceta

ldehyde/A

cid

s

0 2 4 6 8 10 12 20 22Min

8 9

75

6

4

3

2

1 10

Useful for analyses of sewage treatmenteffluents and other wastewater.For trace analyses, use a glass column.For more information, request Bulletin 856.

1. Acetic acid2. Propionic acid3. Isobutyric acid4. n-Butyric acid5. 2-Methylbutyric acid6. 3-Methylbutyric acid7. n-Valeric acid

0 4 12

Min

1410862

7

56

4

3

2

1

8

Figure 5. Free Fatty Acids (C2-C10)Packing: GP 10% SP-1200/1% H3PO4 on 80/100 Chromosorb W AWCat. No.: 11965 (packing, 20g/bottle)Column: 6' x 1/8" stainless steel

Oven: 125°C to 175°C at 15°C/minCarrier: nitrogen, 20mL/min

Det.: FID, 200°CInj.: 0.5µL carbon

disulfide containing0.1% each analyte,150°C

1. C22. C33. iC44. C45. iC56. C57. C68. C89. C101

2

3

6

5

4

8

7

9

1 3 5 6 17 1810Min

713-0963

795-0079

For more information, request Bulletin 856.0 2 4 6 8 10 12

Min

1

2

3

46

5

7 8

For more information,request Bulletin 856.

1. Myristic acid (C14:0)2. Palmitic acid (C16:0)3. Palmitoleic acid (C16:1)4. Stearic acid (C18:0)5. Oleic acid (C18:1)6. Linoleic acid (C18:2)7. Arachidic acid (C20:0)8. Linolenic acid (C18:3)

Figure 6. Free Fatty Acids (C14:0 to C18:3)Packing: 5% DEGS-PS on

100/120 SUPELCOPORTCat. No.: 11870-U (packing)Column: 3' x 2mm ID glass


Det.: FID, 210°CInj.: 1µL Qualmix™ FA

9

713-0962

Figure 7. Whole Milk Free AcidsPacking: 10% SP-216-PS on 100/120 SUPELCOPORTCat. No.: 11879 (packing)Column: 3' x 2mm ID glass

Det: FID, 200°COven: 130°C to 200°C at 15°C/min

Carrier: nitrogen, 20mL/minInj.: 0.5µL carbon disulfide containing 0.1% each analyte, 150°C

Acid

s

Conc. (ppm)U Unknown1. Acetic acid 3702. Propionic acid 783. Isobutyric acid 2824. Butyric acid 1125IS Trimethylacetic acid (int. std.) 505. 2-Methylbutyric acid 2206. Isovaleric acid 2847. Lactic acid 7108. Valeric acid 465

0 4 8 12 16 20Min

1 2 3 4

U

U

IS

5 6

7

8

U

U

12 3

54

7

9

8

10

11 12

0 2 4 6 8 10Min

1. C4:02. C6:03. C8:04. C10:05. C12:06. C14:07. C16:08. C16:19. C18:0

10. C18:111. C18:212. C18:3

6

795-0080For more information, request Bulletin 856.

Figure 8. Lactic Acid in Corn Silage (C2-C5)Packing: 80/120 Carbopack B-DA/4% Carbowax 20MCat. No.: 11889 (packing)Column: 2m x 2mm ID TightSpec™ glass

(stock column available)Oven: 175°C

Carrier: nitrogen, 24mL/minDet.: FID, 200°CInj.: 1µL aqueous mixture, 200°C


10

713-0964

1

2 3

4

56

7

8 9 10

0 5 10 15 20Min

1. Methyl ethyl ketone (int. std.)2. Acetic acid3. Propionic acid4. Acrylic acid (5000ppm)5. Isobutyric acid6. Butyric acid7. Trimethylacetic acid (int. std.)8. 2-Methyl butyric acid9. Isovaleric acid

10. Valeric acid

Figure 9. Lactic Acid in Rumen FluidPacking: 80/120 Carbopack B-DA/4% Carbowax 20MCat. No.: 11889 (packing)Column: 2m x 2mm ID TightSpec glass


Carrier: nitrogen, 24mL/minDet.: FID, 200°CInj.: 1µL aqueous mixture, 200°C

Figure 10. Acrylic/Propionic Separation in C2-C5 AcidsPacking: 80/120 Carbopack B-DA/4% Carbowax 20MCat. No.: 11889 (packing,15g/bottle)Column: 2m x 2mm ID

TightSpec glass(stock column available)


Det.: FID, 200°CInj.: 1µL water, 10ppm each

acid, 100ppm each internal standard, 200°C

Conc. (ppm)U Unknown1. Acetic acid 13002. Propionic acid 10603. Isobutyric acid 434. Butyric acid 313IS Trimethylacetic acid (int. std.) 505. 2-Methylbutyric acid 606. Isovaleric acid 417. Valeric acid 70

Min40 8 12 16 20

U 3

421

IS

56

7

U

U


For more information, request Bulletin 856.795-0082

Lacticacid

(9ppm)

11

Figure 11. Nonvolatile Methylated AcidsPacking: 10% SP-1000/1% H3PO4 on

100/120 Chromosorb W AWCat. No.: 11841 (packing)Column: 6' x 4mm ID glass

Oven: 155°CCarrier: helium, 86mL/min

Figure 12. N-TFA-O-Methyl Esters of D- & L-ValineAmino Acids

Packing: 5% SP-300 on 100/120 SUPELCOPORTCat. No.: 11835-U (packing)Column: 6' x 2mm ID glass


Inj.: 1µL (1µg/µL conc.)


Acid

s

0 2 4 86Min

1

2

3 4 56

8

7

1. Pyruvic acid2. Lactic acid3. Oxalacetic acid4. Oxalic acid5. Methyl malonic acid6. Malonic acid7. Fumaric acid8. Succinic acid

795-0158

0 1 2 3 4 5Min

1

2

1. D-Valine2. L-Valine

12

1

23

4

5 7

6

11

10

9

8

0 2 864 10 12 14Min

1. Dimethyl malonate (C3)2. Dimethyl succinate (C4)3. Dimethyl glutarate (C5)4. Dimethyl adipate (C6)5. Dimethyl pimelate (C7)6. Dimethyl suberate (C8)7. Dimethyl azelate (C9)8. Dimethyl sebacate (C10)9. Dimethyl terephthalate

10. Dimethyl isophthalate11. Dimethyl phthalate

713-0976

795-0159

Figure 13. Methyl Esters of Dibasic AcidsPacking: 10% SP-2340 on 100/120 Chromosorb W AWCat. No.: 11852 (packing)Column: 6' x 1/8" ID stainless steel

Oven: 170°C to 220°Cat 4°C/min

Carrier: nitrogen, 20mL/minInj.: 0.75µL, 10mg/cc

each acid

Figure 14. Bacterial Acid StandardPacking: 3% SP-2100 DOH on 100/120 SUPELCOPORTCat. No.: 12101 (packing)Column: 10' x 2mm ID glass


Det.: FIDInj.: 1µL

0 2 4 6 8 10 12 14 16 18 20Min

For more information, request Bulletin 856 and Application Note 50.

13. 16:014. a -17:015. 17:016. 17:017. 2-OH 16:018. 3-OH 16:019. 18:120. 18:021. 19:022. 19:023. 20:0

1. 11:02. 2-OH 10:03. 12:04. 13:05. 2-OH 12:06. 3-OH 12:07. 14:08. a -15:09. 15:0

10. 2-OH 14:011. 3-OH 14:012. 16:1

1

2

3 4

5

6 7

8

9

10

11

12

13

15

17

18

1619

20

2122

23

14

13

1. Methyl lithocholate (0.94µg/µL)2. Methyl deoxycholate (1.25µg/µL)3. Methyl chenodeoxycholate (1.57µg/µL)4. Methyl cholate (2.50µg/µL)

Min


1

3

2

4

0 4 8 12 16 20

1. Acetaldehyde2. Methanol3. Propanol4. Acetone5. Methyl acetate6. Ethanol7. Isobutanol8. Butanal9. Isopropanol

10. Ethyl acetate11. Diacetal12. n-Propanol13. Isopentanal14. sec-Butanol15. Pentanal16. Ethyl propionate17. Propyl acetate18. Isobutanol19. Acetal

20. Butanol21. Ethyl isobutyrate22. 3-Methylbutan-2-ol23. 3-Pentanol24. 2-Pentanol25. Isobutyl acetate26. Ethyl butyrate27. Butyl acetate28. 2-Methylpentan-1-ol29. 1-Methylpentan-1-ol30. Acetic acid31. Pentanol32. Isoamyl acetate33. Furfural34. Propionic acid35. Hexanol36. Isobutyric acid37. Butyric acid

0 2 4 6 8 10 12 14 16 18 26242220

Min

3

2

1

4

5

6,7

8

9

10

11

12

13

15,16

14

18

20

22

24,25

2617

21

2327

2829

3031

33

3736353432

Figure 16. Alcoholic Beverage/Fermentation ProductsPacking: 80/120 Carbopack B AW/6.6% PEG 20MCat. No.: 11814 (packing, 15g/bottle)Column: 2m x 2mm ID glass

Oven: 80°C to 200°C at 4°C/minCarrier: nitrogen

Det.: FIDInj.: 1µL water:ethanol (50:50),

40-60ppm each analyte

Use this packing in glass columns only.For more information, request Bulletin 790.Figure provided by Dr. Antonio DiCorcia and colleagues, University of Rome (used with permission of ElsevierScientific Publishing Co., Amsterdam). 713-0711

Acid

s, M

eth

yl E

ste

rs/A

lcohols

Figure 15. Bile Acid Methyl EstersPacking: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11878 (packing, 20g/bottle)Column: 3' x 2mm ID glass


Det.: FID

19

14

For more information, request Bulletin 790.Chromatogram provided courtesy of Hayes Separations, Inc.


Figure 18. Cherry BrandyPacking: 80/100 Carbopack B AW/5% Carbowax 20MCat. No.: 11812-U (packing)Column: 2m x 2mm ID glass

(stock column available)Oven: 70°C to 170°C at 5°C/min

Carrier: helium, 20mL/minDet.: FIDInj.: 0.5µL cherry brandy

795-0083

1

2

3

4

5

U

6

10

8

U9

0 2 4 6 8 10 12 14 16Min

7

Figure 17. Tequila HeadspacePacking: HayeSep D, 80/120 meshCat. No.: 10293 (packing)Column: 3' x 1/8" ID stainless steel


Det.: P.E. 900 T.C., 225 ma, 140°CInj.: Valco valve, 100µL, 140°C

3 54210Min

5

6

4

2

13

1. Air (Att. x1)2. Carbon dioxide (Att. x1)3. Water (Att. x1)4. Methanol (Att. x1)5. Acetaldehyde (Att. x1)6. Ethanol (Att. x8)

1. Acetaldehyde2. Methanol3. Ethanol4. Ethyl acetate5. n-PropanolU Unknown6. Isobutanol7. Acetal8. Acetic acid9. Active amyl alcohol

10. Isoamyl alcoholU Unknown

795-0160

15

795-0084

795-0085

Figure 20. Scotch WhiskeyPacking: 80/120 Carbopack B/6.6% Carbowax 20MCat. No.: 11814 (packing)Column: 2m x 2mm ID glass


Det.: FIDInj.: 0.5µL Scotch whiskey

Alc

ohols

1. Acetaldehyde2. Methanol3. Ethanol4. Ethyl acetate5. n-Propanol6. Isobutanol7. Acetic acid8. Active amyl alcohol9. Isoamyl alcohol

1

2

3

6

4

5 7

8

9

0 42 86 10 12 14 16Min

Figure 19. BourbonPacking: 80/120 Carbopack B AW/5% Carbowax 20MCat. No.: 11812-U (packing)Column: 2m x 2mm ID glass


Carrier: helium, 20mL/minDet.: FIDInj.: 0.5µL bourbon whiskey



2 4 8 14 16Min

10 1260

12

4

5 6

8

9

7

3

1. Acetaldehyde2. Methanol3. Ethanol4. Ethyl acetate5. n-Propanol6. Isobutanol7. Active amyl alcohol8. Isoamyl alcohol9. Acetic acid

16


Figure 21. Denaturants in AlcoholPacking: GP 80/100 Carbopack C/0.1% SP-1000Cat. No.: 11820 (packing)Column: 6' x 2mm ID glass


Carrier: nitrogen, 20mL/minDet.: FIDInj.: 70% ethanol & 2% of

each denaturant

Figure 22. Blood AlcoholsPacking: 60/80 Carbopack B/5% Carbowax 20MCat. No.: 11766 ((packing, 15g/bottle)Column: 6' x 2mm ID glass


Carrier: helium, 20mL/minDet.: FIDInj.: 1µL water,

0.05-0.10% each analyte

1. Methanol2. Ethanol3. Acetone4. Isopropanol5. n-Propanol6. tert-Butanol7. Chloroform, methyl ester8. Methyl Cellosolve®

9. sec-Butanol10. Isobutanol11. Ethyl acetate12. n-Butanol13. Ethyl Cellosolve14. Benzene15. Methyl isobutyl ketone

0 2Min

2

34

5

6

7

1

1

4

3

6

5

0 1 2 3 4 5 6Min

2

1. Acetaldehyde2. Methanol3. Acetone4. Ethanol5. Isopropanol6. n-Propanol

For more information, request Application Note 72.713-0622

4 6 8 10

8

9

10

11

13

1214

15

22 24

17

0Min

2 4 6 8 10 12 14

1

3

2

4

5

6

7

8

9

Figure 23. Alcohols (C1-C5) and AcetaldehydePacking: 80/120 Carbopack B AW/5% Carbowax 20MCat. No.: 11812-U (packing, 15g/bottle)Column: 2m x 2mm ID glass


Carrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL Scotch whiskey

Figure 24. Isomeric Alcohols (C1-C5)Packing: 80/100 Carbopack C/0.2% Oronite ® NIWColumn: 2m x 2mm ID glass


Det.: FIDInj.: 0.1µL


Alc

ohols

Use this packing in glass columns only.For more information, request Bulletin 790.

713-0710

1. Acetaldehyde2. Methanol3. Ethanol4. Ethyl acetate5. n-Propanol6. Isobutanol7. Acetic acid8. Active amyl alcohol9. Isoamyl alcohol

0 2 4 6 108

Min

1

2

3

4

6

5

7

10

11

12

9

13

14

16

15

8

1. Methanol2. Ethanol3. 2-Propanol4. 1-Propanol5. 2-Methyl-2-propanol (tert-butyl)6. 2-Butanol (sec-butyl)7. 2-Methyl-1-propanol (isobutyl)8. 1-Butanol9. 2-Methyl-2-butanol (tert-amyl)

10. 2,2-Dimethyl-1-propanol11. 3-Methyl-2-butanol12. 3-Pentanol13. 2-Pentanol14. 2-Methyl-1-butanol (active)15. 3-Methyl-1-butanol16. 1-Pentanol

18

713-1069

Figure 25. Isomeric Alcohols (C1-C5)Packing: 80/100 Carbopack F-SLColumn: 2m x 2mm ID glass



Figure 26. Alcohols (C10-C18)Packing: 10% SP-2100 on 80/100 SUPELCOPORTCat. No.: 12140 (packing)Column: 6' x 1/8" ID stainless steel


Carrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL, 0.1% each

in chloroform


0 21 3 4 5Min

1

2

34

5

6

7

8

1516

13

1412

11

10

9

1. Methanol2. Ethanol3. 2-Propanol4. 1-Propanol5. 2-Methyl-2-propanol (tert-butyl)6. 2-Butanol (sec-butyl)7. 2-Methyl-1-propanol (isobutyl)8. 1-Butanol9. 2-Methyl-2-butanol (tert-amyl)


4 5 6 7 98Min

5

4

3

11. 1-Decanol (C10)2. 1-Dodecanol (C12)3. 1-Tetradecanol (C14)4. 1-Hexadecanol (C16)5. 1-Octadecanol (C18)

0 1 2 3

2


10

19

Figure 27. Alcohols (C14:0-C18:0, C18:1, & C18:2)Packing: 10% SP-2300 on 80/100 SUPELCOPORTCat. No.: 12091 (packing)Column: 6' x 1/8" ID stainless steel


Det.: FIDInj.: 1µL, 1% total sample in chloroform

795-0164

795-0165

Alc

ohols

/Am

ines

0 2 4 6 10 12 14 16 18 20 228

Min

2

1

3

45

6

7

Figure 28. Aliphatic Amines in Water (100ppm)Packing: Carbopack B/4% Carbowax 20M/0.8% KOHCat. No.: 11887 (packing)Column: 6' x 2mm ID glass


Carrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL

1. Methylamine2. Dimethylamine3. Ethylamine4. Trimethylamine5. Isopropylamine6. n-Propylamine7. tert-Butylamine8. Diethylamine9. sec-Butylamine

10. Isobutylamine11. n-Butylamine

0 1 2 3 4 5 6 7 98Min

1

23

4

5 76

8

910

11

10


1. C14:02. C14:13. C16:04. C16:15. C18:06. C18:17. C18:2

20

713-0717


Figure 29. AminesPacking: 80/100 HayeSep BCat. No.: 10286 (packing,75cc/bottle)Column: 5' x 1/8" ID stainless steel

Oven: 140°C to 190°C at 16°C/minCarrier: helium, 30cc/min

Det.: thermal conductivity (175ma, 180°C)Inj.: 0.2µL, on-column injection, 150°C

2

3

2

1

0 1 3 4 5Min

4

5

6

For more information, request Bulletin 737.Chromatogram provided courtesy of Hayes Separations, Inc.

Figure 30. Heterocyclic Amines in Water (1000ppm)Packing: Carbopack B/4% Carbowax 20M/0.8% KOHCat. No.: 11887 (packing)Column: 6' x 2mm ID glass



1. Air2. Water3. Methylamine4. Dimethylamine5. Trimethylamine6. Ethylene diamine

1. Ethylenediamine2. Piperidine3. Pyridine4. Morpholine5. Piperazine6. Cyclohexylamine

0 2 4 6 8 10 12 14 16Min

1

5

6

4

3

2

21

Figure 32. TMS Amino AcidsPacking: 10% OV-11 on 100/120 SUPELCOPORTCat. No.: 11961 (packing)Column: 6' x 2mm ID glass

Oven: 100°C (5 min) to 230°C at 3°C/minCarrier: nitrogen, 30mL/min

795-0167


713-0715

5

4

2IS1

3

0 2 4 6 8 10

Min

11ng Each Amine1. ButylamineIS Triethylamine (int. std.)2. n-Amylamine3. n-Hexylamine4. n-Heptylamine5. n-Octylamine

Am

ines/A

min

o A

cid

s

1. ALA2. GLY

23. IMP4. VAL5. LEU6. GLY

37. PRO8. SER9. THR

10. HYPRO11. ASP12. MET13. GLU

214. GLU

315. PHE16. ARG17. LYS18. TYR19. HIS20. TRP21. CYS

0 8 2416 32 48 5640

Min

2120

19

18

17

16

15

2

1

3

13

12

11

98

5

7

64

10

14

Figure 31. AminesPacking: 60/80 Carbopack B/4% Carbowax 20M/0.8% KOHCat. No.: 11887 (packing, 15g/bottle)Column: 2m x 2mm ID glass

(stock column available)Oven: 100°C (2 min) to 220°C

at 16°C/minCarrier: helium, 30mL/min

Det.: thermionic (nitrogen-specific)

22

Figure 33. AroclorsPacking: 1.5% SP-2250/1.95% SP-2401

on 100/120 SUPELCOPORTCat. No.: 11947 (packing)Column: 2m x 1/4" OD x 4mm ID

TightSpec glass(stock column available)

Oven: 160°C (Aroclor 1016, 1221,1232, 1242, 1248)200°C (Aroclor 1254, 1260)

Carrier: nitrogen, 60mL/minDet.: ECDInj.: 5ng each Aroclor in

5µL isooctane

795-0170For more information, request Bulletin 817and Application Note 67.

0 8 16 24 32 40 48 56Min

Aroclor ® 1016

Aroclor 1221

Aroclor 1232

Aroclor 1242

Aroclor 1248

Aroclor 1254

Aroclor 1260

23

Figure 34. AroclorsPacking: 3% SP-2100 on 100/120

SUPELCOPORTCat. No.: 11738 (packing)Column: 2m x 1/4" OD x 2mm ID TightSpec glass

(stock column available)Oven: 170°C (Aroclor 1016, 1242,

1248, 1254, 1260)140°C (Aroclor 1221, 1232)

Carrier: nitrogen, 40mL/minDet.: ECDInj.: 3ng each Aroclor in 3µL isooctane

Aro

clo

rs

For more information, request Bulletin 817 and Application Note 67.

795-0171

Aroclor 1254

Aroclor 1242

Aroclor 1248

Aroclor 1232

Aroclor 1221

Aroclor 1016

Aroclor 1260

32241680Min

24

1. Air2. Ammonia (35%)3. Water (65%)


Figure 36. Aromatics and AliphaticsPacking: 10% TCEP on 100/120 Chromosorb P AWCat. No.: 12106-U (packing, 20g/bottle)Column: 8' x 1/8" ID stainless steel


Det.: FIDInj.: 0.2µL, approx. equal volumes each analyte

713-0724

Figure 35. AmmoniaPacking: HayeSep P, 60/80 meshCat. No.: 10297 (packing)Column: 8' x 1/8" ID stainless steel


Det.: T.C.(175ma), 200°CInj.: 0.1µL of NH4OH, on-column, 150°C

795-0168

0 1 3Min

2

1

2 3

0 2 4 6 8 10 12 14 16 18Min

1

2

3

4

5

6

78

9,10

1112

1. n-Hexane2. n-Heptane3. n-Octane4. n-Nonane5. n-Decane6. Benzene7. Toluene8. Ethylbenzene9. m-Xylene

10. p-Xylene11. Isopropylbenzene12. o-Xylene


25


Figure 38. Aromatic Impurities in EthylbenzenePacking: 80/100 Carbopack C/0.1% SP-1000Cat. No.: 11820 (packing)Column: 6' x 1/8" ID stainless steelCat. No.: 12495-U (general configuration stock column;

other stock columns available)Oven: 225°C

Carrier: nitrogen, 20mL/min at 42psi


Figure 37. Aromatics in GasolinePacking: 35% BC-150 on 100/120 Chromosorb P AWCat. No.: 11840-U (packing)Column: 10' x 1/8" ID stainless steel


Det.: FIDInj.: 0.5µL gasoline

Am

monia

/Aro

matic

s

1. BenzeneU Unknown2. Toluene3. Ethylbenzene4. Isopropylbenzene5. XylenesU Unknown

0 2 4 6 8 10 12Min

14

1. C1-C12 aliphatic hydrocarbons2. Benzene3. Toluene

1

2

3

0 1 2 43 5 6 7 8Min

1

U 2

3

4 5

U

26



Figure 39. AromaticsPacking: 80/100 Carbopack C/0.1% SP-1000Cat. No.: 11820 (packing)Column: 6' x 1/8" ID stainless steelCat. No.: 12495-U (general configuration stock column;


Carrier: nitrogen, 20mL/min at 42psi

0 1 2 3 4 6 75 98Min

1

2

4

3

5

7

8

61. Benzene2. n-Hexane3. Toluene4. Ethylbenzene5. Isopropylbenzene6. m- & p-Xylene7. o-Xylene8. n-Propylbenzene

Figure 40. AromaticsPacking: 5.0% SP-1200/1.75% Bentone ® 34 on 100/120 SUPELCOPORTCat. No.: 12134 (packing)Column: 6' x 1/8" ID stainless steelCat. No.: 12721 (general configuration stock column;


Carrier: nitrogen, 20mL/minInj.: 0.1µL

0 2 4 6 8 10 12 14 16

Min

18

5

1 2 34

67

89

1. Benzene2. Toluene3. Ethylbenzene4. p-Xylene5. m-Xylene6. o-Xylene7. Isopropylbenzene8. Styrene9. n-Propylbenzene

27


Figure 42. ButylbenzenesPacking: 60/80 Carbopack F-TACat. No.: packing available in packed columns onlyColumn: 2m x 2mm ID glass


Det.: FIDInj.: 0.05µL methyl ethyl ketone

(5% each analyte)

Figure 41. ButylbenzenesPacking: 80/100 Carbopack C/0.1% SP-1000Cat. No.: 11820 (packing, 15g/bottle)Column: 6' x 1/8" ID stainless steelCat. No.: 12495-U (general configuration stock column,


Carrier: nitrogen, 20mL/minDet.: FIDInj.: 1µL chloroform

(~0.1% each analyte)

1. tert-Butylbenzene2. sec-Butylbenzene3. Isobutylbenzene4. n-Butylbenzene

20 4 6Min

4

3

2

1


Aro

matic

s

0 2 4 6 8 10Min

1

2

3

4

1. tert-Butylbenzene2. sec-Butylbenzene3. Isobutylbenzene4. n-Butylbenzene

28

8


Figure 43. Tri- and TetramethylbenzenesPacking: 10% SP-2250 on 100/120 SUPELCOPORTCat. No.: 12132 (packing)Column: 10' x 1/8" ID stainless steel


Inj.: 0.6µL, solution 0.1% each component in CHCl3

Figure 44. Vinyl ChloridePacking: 80/100 Carbopack C/0.19% picric acidCat. No.: 11824 (packing)Column: 6' x 1/8" ID stainless steelCat. No.: 13867 (general configuration stock column only)


Det.: FID, Varian 3700Inj.: 1µL, 1ppm vinyl chloride

in carbon disulfide

1. Vinyl chloride (1ppm)2. Carbon disulfide


6 8 1042 120 14 16 24 26 282220 3018Min

1

23

5 64

1. 1,3,5-Trimethylbenzene2. 1,2,4-Trimethylbenzene3. 1,2,3-Trimethylbenzene4. 1,2,4,5-Tetramethylbenzene5. 1,2,3,5-Tetramethylbenzene6. 1,2,3,4-Tetramethylbenzene

0 1 2 3 4 5 6 7 9

Min

1

2

29

Figure 45. Vinyl ChloridePacking: 60/80 Carbopack C/0.2% Carbowax 1500Cat. No.: 11826 (packing)Column: 6' x 1/8" ID stainless steelCat. No.: 13860-U (general configuration stock column;

other stock columns available)Oven: room temperature

Carrier: nitrogen, 20mL/minDet.: FIDInj.: 1mL nitrogen containing 1ppm VC

0 2 4 6 8

1 2

3

Blank

4

795-0173

Min

713-0694,0695

For more information, request Bulletins 846 and 861.

Chlo

rinate

d H

ydro

carb

ons

Figure 46. Methylene Chloride andVinyl Chloride by OSHA Method 51

Sample: Carboxen™-564 spiked with vinylacetate (18ng) and desorbed withmethanol:methylene chloride (5:95)

Sampling Tube: ORBO™-92Cat. No.: 20362

Packing: 80/100 Carbopack C/0.2% Carbowax 1500

Cat. No.: 11827 (packing, 15g/bottle)Column: 6' x 2mm ID glass


Det.: FIDInj.: 1µL

For more information, requestBulletins 846, and 866.

0 1 2 3

0

10

20

40

30

Min

1. Methanol2. Methylene chloride3. Solvent impurity4. Vinyl acetate

Standard representing0.75mg/m 3 vinyl acetatein 24L air sample

30

Figure 48. Volatile Compounds byUS EPA Methods TO-2 and TO-3

Sample: 1-5ng each componentDesorption Tube: Carbotrap 300, 4mm ID

Cat. No.: 20379Carrier: helium, 20mL/min

Packing: 60/80 Carbopack B/1% SP-1000Cat. No.: 11815 (packing, 15g/bottle)Column: 8' x 1/8" stainless steelCat. No.: 12543 (general configuration stock

column; other stock columns available)Oven: 35°C (2 min) to 220°C at 16°C/minDet.: FID

Figure 47. Chlorinated Volatile Hydrocarbons byUS EPA Method TO-1

Sample: 2ng each componentDesorption Tube: Carbotrap™ 300, 4mm ID

Cat. No.: 20379Thermal Desorption: 330°C, 4 minTransfer Line Temp.: 220°C

Carrier (desorptiontube to column): helium, 20mL/min

Packing: 80/100 Carbopack C/0.1% SP-1000Cat. No.: 11820 (packing, 15g/bottle)Column: 6' x 1/8" stainless steelCat. No.: 12495-U (general configuration stock column;

other stock columns available)Oven: 35°C (2 min) to 220°C at 16°C/min

Carrier: helium, 20mL/minDet.: FID

For more information, request Bulletins 846 and 861.713-0702


0 84 12 16 20Min

1

2

3

4,5

1. Vinyl chloride2. Dichloromethane3. Acrylonitrile4. 1,1-Dichloroethylene5. 3-Chloropropene6. Chloroform7. 1,2-Dichloroethane8. 1,1,1-Trichloroethane9. Carbon tetrachloride

10. Benzene11. Tetrachloroethylene12. Toluene13. Chlorobenzene

6

78

9

10

1112

13

0 4 82 6Min

141210

3

2

4

5

6

7

8

9

1110

1. Methanol (carrier solvent)2. Chloroform3. 1,2-Dichloropropane4. 1,1,1-Trichloroethane5. Carbon tetrachloride6. 1,2-Dichloroethane7. Trichloroethylene8. Bromoform9. Tetrachloroethylene

10. Impurity11. Chlorobenzene

1

31

Figure 50. CreosotePacking: 10% SP-2100 on 100/120 SUPELCOPORTCat. No.: 11989 (packing)Column: 10' x 1/8" ID stainless steelCat. No.: 12717 (general configuration stock column;

other stock columns available)Oven: 100°C to 300°C at 6°C/min


0 4 12 168 20 24 28 32 36 40Min

1

2

3

456

7

8 9

10

11

13

14

1512

For more information, request Bulletin 743 and Application Note 108.713-0749

1. Naphthalene2. 2-Methylnaphthalene3. 1-Methylnaphthalene4. Biphenyl5. 2,6-Dimethylnaphthalene6. 2,3-Dimethylnaphthalene7. Acenaphthalene8. Dibenzofuran9. Fluorene

10. Methyl fluorenes11. Phenanthrene & Anthracene12. Carbazole13. Fluoranthene14. Pyrene15. Chrysene

795-0174

Cre

osote

Min0 2 4 6 81 3 115 7 9 10

11

1 2

3

4

5

6

7

910

8

1. Methylene chloride2. 1,1-Dichloroethane3. Chloroform4. 1,2-Dichloroethane5. 1,1,1-Trichloroethane6. Carbon tetrachloride7. Trichloroethylene8. 1,1,2-Trichloroethane9. Perchloroethylene

10. 1,1,1,2-Tetrachloroethane11. 1,1,2,2-Tetrachloroethane


Figure 49. Chlorinated SolventsPacking: 20% SP-2100/0.1% Carbowax 1500 on 100/120 SUPELCOPORTCat. No.: 11821 (packing)Column: 10' x 1/8" ID stainless steelCat. No.: 12718-U (general configuration stock column;


Carrier: nitrogen, 20mL/minDet.: FID

32



Figure 52. Creosote with Liquid Crystal PhasePacking: 1.5% SP-2100/1% BMBT on 100/120 SUPELCOPORTCat. No.: customColumn: 10' x 1/8" ID stainless steel


Det.: FIDInj.: 0.1µL creosote

Figure 51. CreosotePacking: 10% SP-2250 on 100/120 SUPELCOPORTCat. No.: 12132 (packing)Column: 10' x 1/8" ID stainless steel




10. Methyl fluorenes11. Phenanthrene & Anthracene12. Carbazole13. Fluoranthene14. Pyrene

0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60Min

1

2

3

4

6

7

8

9

11

12

13

14

10

5


10. Methyl fluorenes11. Phenanthrene12. Anthracene13. Carbazole14. Fluoranthene15. Pyrene

0 4 12 24 28Min

8 322016

1

2

3

5

4

7

6

89

1012

11

13 14 15

3 3

Figure 54. NicotinePacking: 10% Carbowax 20M/2% KOH

on 80/100 Chromosorb W AWCat. No.: 11805 (packing)Column: 6' x 2mm ID

Oven: 200°CCarrier: 20mL/min

Inj.: 1µL

Figure 53. Cannibinoids as TMS DerivativesPacking: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11875 (packing)Column: 6' x 2mm ID glass


Det.: FID, 275°C

1. Cannabidiol2. Cannabichromene3. Cannabigerol4. D 6-THC5. D 1-THC6. Cannabinol

795-0176

Cre

osote

/Dru

gs


0 2 4 6 108Min

5

3

2

1

6

Nicotine

0 1 2 3 4Min

4

3 4

Figure 55. Anticonvulsant Drugs, UnderivatizedPacking: 2% SP-2110/1% SP-2510-DA on 100/120 SUPELCOPORTCat. No.: 11768 (packing, 20g/bottle)Column: 3' x 2mm ID glass

Oven: 120°C to 250°C at 16°C/min, hold 4 minCarrier: helium, 50mL/min

Det.: FIDInj.: 2µL, analyte amounts shown on figure

Figure 56. Anticonvulsant Drugs, UnderivatizedPacking: 2% SP-2510-DA on 100/120 SUPELCOPORTCat. No.: 11776 (packing, 20g/bottle)Column: 3' x 2mm ID glass

Oven: 150°C to 265°C at 16°C/min,hold 4 min

Carrier: nitrogen, 50mL/minDet.: FIDInj.: 2µL

1. Ethosuximide2. Methsuximide3. Phensuximide4. Barbital (int. std.)5. N-Desmethyl methsuximide6. Mephenytoin7. N-Desmethyl phensuximide8. Phenylethylmalonamide9. 5-Methyl-5-phenythydantoin (int. std.)

10. Phenobarbital11. Carbamazepine12. Primidone13. Diphenythydantoin14. 5-p-Methylphenyl-5-phenythydantoin

(int. std.)

713-0626

2

3

4 5

6

71

8

9

10

11

12 13

14

0 1 2 3 4 5 6 7 8 9Min

1110

2 4 6 8 10 12

3

4

6

5

11

78

9

10

1

2

0Min

1. Ethosuximide, 1.0µg2. a -Methyl,a -propyl succinimide (int. std.), 1.0µg3. Phenylethylmalonamide (PEMA), 0.2µg4. p-Methyl PEMA (int. std.), 0.2µg5. Phenobarbital, 0.5µg6. p-Methyl phenobarbital (int. std.), 0.5µg7. Carbamazepine, 0.2µg8. Promidone, 0.2µg9. p-Methyl primidone (int. std.), 0.2µg

10. Phenytoin, 0.3µg11. p-Methyl phenytoin (int. std.), 0.5µg

713-0625

3 5

Figure 58. Psychotropic Drugs, Antidepressants,Underivatized

Packing: 3% SP-2250 on 80/100 SUPELCOPORTCat. No.: 11767 (packing, 20g/bottle)Column: 6' x 2mm ID glass


Det.: NPDInj.: 5µL methanol,

10ng each drug

Figure 57. Anticonvulsant DrugsPacking: 10% SP-1000 on 80/100 SUPELCOPORTCat. No.: 11872 (packing)Column: 3' x 2mm ID glass


Det.: FID, 250°CInj.: 0.25µg/mL each drug

713-0675

713-0676

1

3

4

2

0 1 2 43 5Min

1. Valproic acid2. Cyclohexane carboxylic acid3. a ,a -Dimethyl-b -methylsuccinimide4. Ethosuximide

1. Amitriptyline2. Nortriptyline3. Clomipramine

1

2

3

0 2 4 6Min

Dru

gs

3 6

Figure 59. BarbituratesPacking: 3% SP-2250 DA on 100/120 SUPELCOPORTCat. No.: 11981 (packing)Column: 6' x 2mm ID glass


Inj.: 1µL, 0.1µg/µL each component

1. Amobarbital2. Pentobarbital3. Secobarbital4. Phenobarbital

Figure 60. AlkaloidsPacking: 3% SP-2250 DB on 100/120 SUPELCOPORTCat. No.: 11983 (packing, 20g/bottle)Column: 3' x 2mm ID glass


Det.: FIDInj.: 1µL methanol, analyte quantities shown on figure

713-0664

795-0178

0 1 2 43

Min

4

3

1

2

1. Procaine, 0.2µg2. Methadone, 0.2µg3. Cocaine, 0.4µg4. Codeine, 0.4µg5. Heroin, 0.4µg6. Quinine, 2.0µg

1

2

3

4

56

0 2 3 4 5 6 7 8 9 10Min

3 7

Figure 61. US EPA Method 601, Purgeable HalocarbonsPacking: 60/80 Carbopack B/1% SP-1000Cat. No.: 11815 (packing)Column: 8' x 1/8" OD stainless steelCat. No.: 12543 (general configuration stock column; other stock columns available)

Oven: 45°C (3 min) to 220°C at 8°C/min, 15 min holdCarrier: nitrogen, 40mL/min

Det.: Hall®, 85°CInj.: 1µL of a synthetic mixture of volatile pollutants in dodecane, 200-500ng/µL

each component, 200°C

EP

A M

eth

ods (6

00

Serie

s)

0 2 4 6 108 12 14 16 18 20 22MinFor more information, request

Bulletins 775 and 865.795-0179

1 2

4

35

7

IS

6

89

10

11

13

14

IS

17

19

20

IS18

12 15

16

21

For more information, requestBulletins 775 and 865.

795-0180

1. Chloromethane2. Bromomethane3. Vinyl chloride & Dichlorofluoromethane4. Chloroethane5. Methylene chloride6. Trichlorofluoromethane7. 1,1-DichloroethyleneIS Bromochloromethane (int. std.)8. 1,1-Dichloroethane9. trans-1,2-Dichloroethylene

10. Chloroform11. 1,2-Dichloroethane12. 1,1,1-Trichloroethane13. Carbon tetrachloride14. Bromodichloromethane15. 1,2-Dichloropropane &

trans-1,3-Dichloropropene16. Trichloroethylene

17. cis-1,3-Dichloropropene, 1,1,2-Trichloroethane, &Chlorodibromomethane

IS 1-Chloro-2-bromopropane (int. std.)18. 2-Chloroethyl vinyl ether19. Bromoform20. 1,1,2,2-Tetrachloroethylene & 1,1,2,2-Tetrachloro-

ethaneIS 1,4-Dichlorobutane (int. std.)21. Chlorobenzene

0Min

2 4 6 8 10 12 14 16 18 20 22

1. Benzene2. Toluene3. Ethylbenzene4. Chlorobenzene5. 1,4-Dichlorobenzene6. 1,3-Dichlorobenzene7. 1,2-Dichlorobenzene

1

32 4

5 67

Figure 62. US EPA Method 602, Purgeable AromaticsPacking: 5% SP-1200/1.75% Bentone 34 on 100/120 SUPELCOPORTCat. No.: 12134 (packing)Column: 6' x 1/8" ID stainless steelCat. No.: 12721 (general configuration stock column; other stock columns available)

Oven: 50°C (2 min) to 90°C at 6°C/min and holdCarrier: helium, 36mL/min

Det.: FIDInj.: 1µL of 0.2mg/mL standard of each purgeable aromatic in methanol

3 8

Figure 64. US EPA Method 604, Derivatives of PhenolsPacking: 5% SP-2250 on 80/100 SUPELCOPORTCat. No.: 11737 (packing)Column: 1.8m x 2mm ID glass


Det.: ECInj.: 0.1µL with 0.2µg/mL of each component in methanol

Figure 63. US EPA Method 603, Acrolein and AcrylonitrilePacking: 80/100 Chromosorb 101Cat. No.: 20214 (packing)Column: 6' x 1/8" OD stainless steelCat. No.: 12712 (general configuration stock column; other stock columns available)


Det.: FIDInj.: 2µL synthetic standard in water


1. Acrolein, 3mg/mL2. Acrylonitrile, 1mg/mL

0 2 4 6 8 10 12

Min

1

2


0 2 4 6 8 10 12MIn

2

1

3

45

6 7

8

9

1. Phenol2. 2,4-Dimethylphenol3. 2-Chlorophenol4. 4-Methyl-3-chlorophenol5. 2,4-Dichlorophenol6. 2,4,6-Trichlorophenol7. 2-Nitrophenol8. 4-Nitrophenol9. Pentachlorophenol

3 9

Figure 65. US EPA Method 604, PhenolsPacking: 1% SP-1240-DA on 100/120 SUPELCOPORTCat. No.: 11832 (packing, 20g/bottle)Column: 2m x 2mm ID glass


Det.: FID, 250°CInj.: 0.5µL EPA Phenols Mix (Cat. No. 48859)


Figure 66. US EPA Method 606, PhthalatesPacking: 1.5% SP-2250/1.95% SP-2401 on 100/120 SUPELCOPORTCat. No.: 11947 (packing)Column: 2m x 4mm ID glass (stock column available)

Oven: 180°C and 220°CCarrier: nitrogen, 60mL/min

Det.: ECD, 250°CInj.: 1µL of phthalate mixture at concentrations indicated


1. Methanol2. o-Chlorophenol (0.25µg)3. 2-Nitrophenol (0.25µg)4. Phenol (0.25µg)5. 2,4-Dimethylphenol (0.25µg)6. 2,4-Dichlorophenol (0.25µg)7. 2,4,6-Trichlorophenol (0.75µg)8. 4-Chloro-m-cresol (1.25µg)9. 2,4-Dinitrophenol (0.75µg)

10. 4,6-Dinitro-p-cresol (1.25µg)11. Pentachlorophenol (1.25µg)12. Nitrophenol (1.25µg)

0 2 4 6 8 10 12 14 16 18 20 22 24Min

1

32

4 5

6

7

8

9

10

11

12

EP

A M

eth

ods (6

00

Serie

s)

1. Dimethyl phthalate (1.0ppm)2. Diethyl phthalate (0.2ppm)3. Di-n-butyl phthalate (1.0ppm)4. Butyl benzyl phthalate (1.0ppm)5. bis(2-Ethylhexyl) phthalate (0.4ppm)6. Di-n-octyl phthalate (1.0ppm)

0 8642 0 2 64 8 10 12 14 16 2018Min

180°C 220°C

1

2 3

5

4

6

MIn

4 0

Figure 67. PhthalatesPacking: 5% SP-2100 on 100/120 SUPELCOPORTCat. No.: 11782-U (packing)Column: 6' x 1/8" stainless steel


Det.: FIDInj.: 1µL of 75ng/µL each component in carbon disulfide

Figure 68. US EPA Method 607, NitrosaminesPacking: 10% Carbowax 20M/2% KOH on 80/100 Chromosorb W AWCat. No.: 11739 (packing)Column: 2m x 4mm ID glass

Oven: 110°C and 220°CCarrier: helium, 40mL/min

Det.: FIDInj.: 2µL of 0.2mg/mL standard in methanol at concentrations indicated


0 2 4 6 8 10 12 14 16

Min

1

2

S

S Carbon disulfide (solvent)1. Di-n-butyl phthalate2. bis(2-Ethylhexyl)phthalate

795-0185

200 4 8 12

1

2

3

0 1616 24 20Min Min

110°C 220°C

1. N-Nitrosodimethylamine (0.2mg/mL)2. N-Nitroso-di-n-propylamine (0.2mg/mL)3. N-Nitrosodiphenylamine

(as Diphenylamine) (0.4mg/mL)

4 8 12 24For more information, requestBulletins 775 and 865.

4 1

Figure 69. N-NitrosaminesPacking: 10% Carbowax 20M/2% KOH on

80/100 Chromosorb W AWCat. No.: 11739 (packing)Column: 2m x 4mm ID TightSpec glass


Det.: Hewlett-Packard NPDInj.: 1ng each componetnt in

2µL methylene chloride

Figure 70. US EPA Method 608, PesticidesPacking: 1.5% SP-2250/1.95% SP-2401 on 100/120 SUPELCOPORTCat. No.: 11947 (packing)Column: 2m x 4mm ID glass


Carrier: argon:methane(95:5), 6mL/min

Det.: EC Ni63Inj.: 0.5µL of 0.02mg/mL

of each pesticide



EP

A M

eth

ods (6

00

Serie

s)

1. N-Nitrosodimethylamine2. N-Nitrosodi-n-propylamine

1

20 4 6 8 10

2

Min

1. a -BHC2. g -BHC3. b -BHC4. Heptachlor5. d -BHC6. Aldrin7. Heptachlorepoxide8. Endosulfan I9. 4,4'-DDE

10. Dieldrin11. Endrin12. 4,4'-DDD & Endosulfan II13. 4,4'-DDT14. Endrin aldehyde15. Endosulfan sulfate

18Min

16121086420 14

1

2

4

6

73

5

8

910

11 12

131514

4 2

Figure 72. US EPA Method 610,Polynuclear Aromatic Hydrocarbons

Packing: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11744 (packing)Column: 2m x 2mm ID glass

Oven: 100°C (4 min) to 280°C at 8°C/min and holdCarrier: nitrogen, 40mL/min

Det.: FIDInj.: 0.2µg each component in 1µL methanol/methylene chloride

Figure 71. US EPA Method 609,Nitroaromatics and Isophorone

Packing: 1.5% SP-2250/1.95% SP-2401 on 100/120 SUPELCOPORTCat. No.: 11947 (packing)Column: 2m x 4mm ID glass

(stock column available)Oven: 85°C and 145°C

Carrier: nitrogen, 44mL/minDet.: FID for nitrobenzene &

isophoroneEC for dinitrotoluenes

Inj.: 2µL of 0.2mg/mLstandard on FID0.5µL of 2ng/µLstandard on EC


85°C 145°C

1. Nitrobenzene2. Isophorone3. 2,6-Dinitrotoluene4. 2,4-Dinitrotoluene

0 2 64 8 0 2 4 6 8 10MinMin

1

23

4

1. Naphthalene2. Acenaphthylene3. Acenaphthene4. Fluorene5. Phenanthrene & Anthracene6. Fluoranthene7. Pyrene8. Benzo(a)anthracene9. Chrysene

10. Benzo(b)fluoranthene & Benzo(k)fluoranthene11. Benzo(a)pyrene12. Dibenz(a,h)anthracene & Indeno(1,2,3-cd)pyrene13. Benzo(ghi)perylene

795-0189

For more information,request Bulletin 775.0 4 8 12 16 20 24 28 32 36 40 4844

Min

1

23 4

5

76

11

1213

8109

4 3

Figure 74. US EPA Method 612, Chlorinated HydrocarbonsPacking: 1.5% OV-1/1.5% OV-225 on 80/100 SUPELCOPORTCat. No.: custom (packing)Column: 2m x 2mm ID glass

Oven: 75°C and 160°CCarrier: argon:methane (95:5), 30mL/min

Det.: ECInj.: 2µL of synthetic mixture (0.1 to 10ng/µL) in hexane

1. bis(2-Chloroisopropyl)ether2. 4-Chlorophenyl phenyl ether3. bis(2-Chloroethoxy)methane4. bis(2-Chloroethyl)ether5. 4-Bromophenyl phenyl ether

Figure 73. US EPA Method 611, HaloethersPacking: 3% SP-1000 on 100/120 SUPELCOPORTCat. No.: 11746 (packing)Column: 2m x 2mm ID glass


Det.: FIDInj.: 1µL of 0.2mg/mL of each haloether in methanol

EP

A M

eth

ods (6

00

Serie

s)


0 2 4 6 8 10 12 0 4 62 8Min MinFor more information, request

Bulletins 775 and 865.795-0191

75°C 160°C

14

3

5

2

6

9

8

7

1. 1,3-Dichlorobenzene2. 1,4-Dichlorobenzene3. Hexachloroethane4. 1,2-Dichlorobenzene5. Hexachlorobutadiene6. 1,2,4-Trichlorocyclopentadiene7. Hexachlorocyclopentadiene8. 2-Chloronaphthalene9. Hexachlorobenzene

12Min

20 4 6 8 10 1814 16 20 22

532

1

4

4 4

713-1037

Figure 75. US EPA Method 625, Base/NeutralsPacking: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11756 (packing)Column: 2m x 2mm ID glass


Det.: FID, 300°CInj.: 1µL methylene chloride, 0.125µg

or 0.063µg (*) each analyte

1. 1,3-Dichlorobenzene2. 1,4-Dichlorobenzene-d43. Bis(2-chloroethyl)ether4. Hexachloroethane & 1,2-Dichlorobenzene5. Bis(methyl-2-chloroethyl)ether6. N-Nitroso-di-n-propylamine7. Nitrobenzene & Hexachlorobutadiene8. 1,2,4-Trichlorobenzene & Isophorone9. Naphthalene & Bis(2-chloroethoxy)methane

10. Hexachlorocyclopentadiene11. 2-Chloronaphthalene12. Acenaphthylene13. Acenaphthene14. Dimethylphthalate15. 2,6-Dinitrotoluene

16. Fluorene & 4-Chlorophenylphenyl ether17. 2,4-Dinitrotoluene18. Diethylphthalate & N-Nitrosodiphenylamine19. 4-Bromophenylphenyl ether &

Hexachlorobenzene20. Phenanthrene & Anthracene21. Di-n-butyl phthalate22. Fluoranthene*23. Pyrene*24. Benzidine25. Butyl benzyl phthalate26. Bis(2-ethylhexyl)phthalate27. Benzo(a)fluoranthene*28. Chrysene*29. 3,3'-Dichlorobenzidine30. Di-n-octyl phthalate31. Benzo(b)fluoranthene* & Benzo(k)fluoranthene*32. Benzo(a)pyrene33. Indeno(1,2,3-cd)pyrene* &

Dibenzo(a,h)anthracene*34. Benzo(ghi)perylene*

3

4

56

7 8

9

10

11

1312

14 15

1617

18

19

20

21272322

24

2526 28

3029

31

33 3432

21

1

2

3

5

6

7

8

4

109

11

1213 14

1516

17

1918 20

21 22

23

24

25

26

27 28

313230

29

0 4 8 12 16 20 24 28 32 36 40 44

Min

Figure 76. US EPA Method 624, VolatilesPacking: 60/80 Carbopack B/1% SP-1000Cat. No.: 11815 (packing, 15g/bottle)Column: 8' x 1/8" stainless steelCat. No.: 12543 (general configuration stock column; other stock columns available)

Oven: 45°C (3 min) to 220°C at 8°C/min (hold 15 min)Carrier: helium, 40mL/min

Det.: FID, 250°CInj.: 1µL of a synthetic mixture of volatile

pollutants in dodecane,200-500ng/µLeach, 200°C


713-0704

0 4 8 12 16 20 24 28 32 36 52484440Min


1. Chloromethane2. Methanol3. Bromomethane4. Vinyl chloride,

Dichlorofluoromethane5. Chloroethane6. Methylene chloride7. Trichlorofluoromethane8. 1,1-Dichloroethylene9. Bromochloromethane (IS)

10. 1,1-Dichloroethane

11. trans-1,2-Dichloroethylene12. Chloroform13. 1,2-Dichloroethane14. 1,1,1-Trichloroethane15. Carbon tetrachloride16. Bromodichloromethane17. 1,2-Dichloropropane18. trans-1,3-Dichloropropene19. Trichloroethylene20. Benzene21. cis-1,3-Dichloropropene,

1,1,2-Trichloroethane,Chlorodibromomethane

22. 1-Chloro-2-bromopropene (IS)23. 2-Chloroethyl vinyl ether24. Bromoform25. 1,1,2,2-Tetrachloroethylene,

1,1,2,2-Tetrachloroethane26. 1,4-Dichlorobutane (IS)27. Toluene28. Chlorobenzene29. Ethylbenzene30. 1,3-Dichlorobenzene31. 1,2-Dichlorobenzene32. 1,4-Dichlorobenzene

4 5


1. Methanol2. o-Chlorophenol, 0.5mg/mL3. 2-Nitrophenol, 0.5mg/mL4. Phenol, 0.5mg/mL5. 2,4-Dimethylphenol, 0.5mg/mL6. 2,4-Dichlorophenol, 0.5mg/mL7. 2,4,6-Trichlorophenol, 1.5mg/mL8. 4-Chloro-m-cresol, 2.5mg/mL9. 2,4-Dinitrophenol, 1.5mg/mL

10. 4,6-Dinitro-o-cresol, 2.5mg/mL11. Pentachlorophenol, 2.5mg/mL12. 4-Nitrophenol, 2.5mg/mL

Figure 77. US EPA Method 625, PhenolsPacking: 1% SP-1240-DA on 100/120 SUPELCOPORTCat. No.: 11832 (packing)Column: 2m x 2mm ID glass

Oven: 70°C (2 min) to 200°C at 8°C/minCarrier: helium, 30mL/min

Det.: FIDInj.: 0.5µL of a standard phenol mixture (Cat. No. 48859)

Figure 78. US EPA Method 625, PesticidesPacking: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11878 (packing)Column: 2m x 2mm ID glass


Det.: FIDInj.: 0.1µL of a synthetic mixture of pesticides (1ng/µL each)

EP

A M

eth

ods (6

00

Serie

s)

1. a -BHC2. g -BHC3. b -BHC4. Heptachlor5. d -BHC6. Aldrin7. Heptachlorepoxide8. Endosulfan I9. Dieldrin & 4,4'-DDE

10. Endrin11. Endosulfan II & 4,4'-DDD12. 4,4'-DDT13. Endosulfan sulfate & Endrin aldehyde

0 2 4 6 8 10 12 14 16 18 20 2422Min

12

11

10

9

8

7

6

54

32

1

20 6 108 1412 18 22 2624 28 302016 3432Min

1

1213

11

10

76

2

4

53

8

9


4

4 6


Figure 79. Ethylene Oxide and Ethylene Oxide ResiduesPacking: 80/100 Carbopack C/0.8% THEEDCat. No.: 11880-U (packing)Column: 1m x 2mm ID glass


Carrier: nitrogen, 20mL/minDet.: FIDInj.: 1µL water containing

50ppm each component1. Ethylene oxide2. Water3. Ethylene chlorohydrin4. Ethylene glycol

2

0 1 2 3 4 5 6Min

4

3

1. Ethylene oxide, 23ppm


Figure 80. Trace Ethylene Oxide in NitrogenPacking: HayeSep DCat. No.: 10292 (packing)Column: 10' x 1/8" stainless steel


Det.: FID, 140°CInj.: 250mL Valco valve, 100°C

1

5 4 3 2 1 0

Min

1

4 7

Figure 81. Ethylene Oxide by US Army MethodSample: HBr-coated Carboxen-564 spiked

with 2-bromoethanol (1.2ng) anddesorbed with toluene:acetonitrile (50:50)

Sampling Tube: ORBO-78Cat. No.: 20355Packing: 10% DEGS on 80/100 SUPELCOPORTCat. No.: 11999 (packing, 20g/bottle)Column: 12' x 1/8" stainless steel


Det.: ECD

Figure 82. Fatty Acid Methyl Esters of Rapeseed Oil MixturePacking: 5% DEGS-PS on 100/120 SUPELCOPORTCat. No.: 11870-U (packing, 20g/bottle)Column: 6' x 1/8" stainless steel


Det.: FIDInj.: 0.5µL RM-3 Mix (Cat. No. O7256-1AMP), 10µg FAMEs/µL in chloroform


713-0973

1. C14:02. C16:03. C18:04. C18:15. C18:26. C20:07. C18:38. C22:09. C22:1

10. C24:0

0 1 2 3 4 5 6 7 8 9Min

1

2

3

4

5

67 8

9

10

Eth

yle

ne O

xid

e/F

atty

Acid

s

S SolventI Solvent impurities

2-Bromoethanol

0 2 4Min

I

S

2-Bromoethanol back-ground is negligible

Blank

EtO AnalysesI

I

I

For more information, request Application Note 109.713-0696, 0697

4 8

Figure 83. Fatty Acid Methyl Esters of Rapeseed Oil MixturePacking: GP 10% DEGS-PS on 80/100 SUPELCOPORTCat. No.: 11999 (packing, 20g/bottle)Column: 6' x 1/8" stainless steel


Det.: FIDInj.: 0.5µL RM-3 Mix (Cat. No. O7256-1AMP), 10µg FAMEs/µL in chloroform

Figure 84. C18:3, C20:0, and C20:1 Methyl EstersPacking: GP 3% SP-2310/2% SP-2300 on 100/120 Chromosorb W AWCat. No.: 11833 (packing)Column: 6' x 1/8" stainless steel


Det.: FIDInj.: 5µg mixed esters in 0.5µL methylene chloride

1. C14:02. C16:03. C18:04. C18:15. C18:26. C20:07. C18:38. C22:09. C22:1

10. C24:0

For more information, requestBulletin 856.

713-1072

0 2 4 6 8 10 12 14 16 18MIn

1

2

3

4

5

76

8

9

10


Min

0 2 4 6 8 10 12 14 16 18 20

1

3

24 5

6

7

8

9

10

11

1. C14:02. C16:03. C18:04. C18:15. C18:26. C18:37. C20:08. C20:19. C22:0

10. C22:111. C24:0

49


795-0197

Figure 85. Methyl Elaidate and Oleate FAMEsPacking: 15% OV-275 on 100/120 Chromosorb P AW DMCSCat. No.: 11844-U (packing, 20g/bottle)Column: 20' x 1/8" stainless steel


Det.: FIDInj.: 0.5µL isooctane containing 5µg each analyte

Figure 86. Whole Milk Free AcidsPacking: 10% SP-216-PS on 100/120 SUPELCOPORTCat. No.: 11879 (packing)Column: 3' x 2mm ID glass


1. Methyl elaidate2. Methyl oleate


0 4 8 12 16 20 24 28 32 36 40Min

1 2

0 1 2 3 4 5 6 7 8 9 10 11Min

1. C4:02. C6:03. C8:04. C10:05. C12:06. C14:07. C16:08. C16:19. C18:0

10. C18:111. C18:212. C18:3

12 3 4

5

6

7

8

9

10

1112

Fatty

Acid

Meth

yl E

ste

rs

50

2

Figure 87. C18:3, C20:0, and C20:1 Methyl EstersPacking: GP 3% SP-2310/2% SP-2300 on 100/120 Chromosorb W AWCat. No.: 11833 (packing)Column: 6' x 1/8" stainless steel


Det.: FIDInj.: 5µg mixed esters in 0.5µL methylene chloride

Figure 88. Polyunsaturated FAMEsPacking: 10% SP-2330 on 100/120 Chromosorb W AWCat. No.: 11851 (packing)Column: 6' x 1/8" stainless steelCat. No.: 13775 (general configuration stock column; other stock columns available)

Oven: 210°CInj.: 15µg mixed esters in 1.5µL isooctane



2 4 60 8 10 12 14 16 18 20MIn

1

3

54

6

7

8

9

10

11

1. C14:02. C16:03. C18:04. C18:15. C18:26. C18:37. C20:08. C20:19. C22:0

10. C22:111. C24:0

1. C14:02. C16:03. C18:04. C18:15. C20:16. C22:17. C20:58. C24:19. C22:5

10. C22:6

0 16MIn

1814122 4 6 8 10

1

2

3 4

5

6

7

8

10

9

51

Figure 89. Reference Standard: RM-3 Peanut Oil TypePacking: 10% SP-2340 on 100/120 Chromosorb W AWCat. No.: 11852 (packing)Column: 2 meter x 2mm ID glass


Inj.: 0.5µL, 10mg/cc isooctane

1. C14:02. C16:03. C18:04. C18:15. C18:26. C18:37. C20:08. C22:09. C22:1

10. C24:0

795-0201

Figure 90. Grapefruit OilPacking: 5% SP-2100/0.1% SP-401 on 100/120 SUPELCOPORTCat. No.: 11839 (packing)Column: 6' x 2mm ID glass



FA

MEs/F

ragra

nces


1. a -Pinene2. Sabinene3. Myrcene4. Limonene5. Unknown6. Nonanal7. Linalool8. Citronellal9. Decanal

10. Unknown11. a -Copaene12. b -Copaene13. b -Elemene14. Caryophyllene15. Humulene16. Valencene17. D-Cadinene18. Nootkatone

0 2 4 6 8 10 12 14 16Min

2

1

4

3

5

67 8

9

10

1 32 4

6,7

58

9

10 11

12

13

14

18

15 1617

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19Min

52

Figure 91. Lemon OilPacking: 5% SP-2100/0.1% SP-401 on 100/120 SUPELCOPORTCat. No.: 11839 (packing)Column: 6' x 2mm ID glass



795-0202

Figure 92. Orange OilPacking: 5% SP-2100/0.1% SP-401 on 100/120 SUPELCOPORTCat. No.: 11839 (packing)Column: 6' x 2mm ID glass




0 2 4 106 8 12 14 16 2018 22 24 26 3028 32 34 36 38Min

1 2 3

4

5

7

8

9

10

1112 1713

61615

14 1918

0Min

2

1. a -Thujene2. a -Pinene3. Camphene4. Sabinene5. b -Pinene6. Myrcene7. Unknown8. p-Cymene9. Limonene

10. g -Terpinene11. Terpinolene12. Nonanal13. Linalool

14. Unknown15. Terpinene-4-ol16. a -Terpineol17. Neral18. Geranial19. Neryl acetate20. Geranyl acetate21. Caryophyllene22. Bergamotene23. Unknown24. Unknown25. b -Bisabolene

302826242220181614121084 6

1

2

3

4,5 6

7

8

9 10

11

1415

16

17

18

19

20

21

22

25

12,13

23

24

1. a -Pinene2. Sabinene3. Myrcene/Octanal4. Phellandrene5. Limonene6. Unknown7. Nonanal8. Linalool9. Citronellal

10. Decanal11. Neral12. Geranial13. Dodecanal14. Caryophyllene15. b -Copaene16. Farnesene17. Valencene18. b -Sinensal19. a -Sinensal

53

Figure 93. Freon ® and Chlorinated HydrocarbonsPacking: 80/100 Carbopack B/1% SP-1000Cat. No.: 11815 (packing)Column: 6' x 1/8" stainless steel

Cat . No.: 12485 (general configuration stock column;other stock columns available)


Det.: FID

Figure 94. Freon GasesPacking: 60/80 Carbopack C/0.2% Carbowax 1500Cat. No.: 11826 (packing)Column: 6' x 1/8" stainless steel

Cat . No.: 13860-U (general configuration stock column;other stock columns available)


Det.: FID



Fra

gra

nces/F

reons

1. Methyl chloride2. Methyl bromide3. Vinyl chloride & Freon 124. Chloroethane

0 1 2 3 4

Min

4

3

2

1

1. Freon 232. Freon 133. Freon 224. Freon 1155. Freon 126. Freon 217. Freon 1148. Freon 119. Freon 113

0 2 4 6 8 10 12Min

9

8

7

6

5

4

23

1

54

Figure 95. Freon GasesPacking: 60/80 Carbopack B/5% Fluorcol ®

Column: 10' x 1/8" SP alloyCat. No.: 12425

(general configuration stock column only)Oven: 50°C

Carrier: nitrogen, 30mL/minDet.: FIDInj.: 10µL synthetic mixture (appr. 20% each compound)

For more information, request Bulletin 786.Figure provided by J.L. Glajch and W.G. Schindel, I.I. du Pont de Nemours and Co., Inc.,Experimental Station, Wilmington, DE, USA.

713-0705

Figure 96. FormaldehydePacking: 100/120 HayeSep TCat. No.: 10311 (packing, 75cc/bottle)Column: 5' x 1/8" stainless steel


Det.: thermal conductivity (175mA)Inj.: 0.2µL (165°C)

713-0690

1. Freon 222. Freon 123. Freon 214. Freon 1145. Freon 114a (impurity)6. Freon 11

0 2 4 6 8 10 12 14 16 18 20Min

3

2

4

65

1. Air2. Formaldehyde3. Water4. Methanol

0 1 2 3Min

1

2

4

3

1

55

Figure 97. Formalin SolutionPacking: 45/60 Carboxen-1000Column: 2' x 1/8" stainless steelCat. No.: 12370-U (general configuration stock

column; other stock columns available)Oven: 150°C

Carrier: helium, 30mL/minDet.: TCDInj.: 0.2µL formalin solution

795-0206

Figure 98. Ethylene and Scott ® Mix 216Packing: 100/120 HayeSep DBCat. No.: customColumn: 30' x 1/8" stainless steel


Det.: P.E. 900 T.C., 300maInj.: 250mL Valco valve, 120°C


Fre

ons/F

orm

ald

ehyde/C

1-C

2

2

3

1

1. Water2. Formaldehyde3. Methanol

0 2 4Min

1. Nitrogen2. Carbon monoxide3. Methane4. Carbon dioxide5. Acetylene (0.24ppm)6. Ethylene7. Water8. Ethane

0 2 3 4 5 6 7 98 10 11 12 13Min

1

87

6

5

3

1

4

2

56

Figure 99. Carbon Dioxide and C2 HydrocarbonsPacking: HayeSep TCat. No.: 10311 (packing)Column: 5' x 1/8" stainless steel


Det.: 180°CInj.: 50mL


Figure 100. Mapp Gases, C1-C3 UnsaturatedHydrocarbons and CO 2

Packing: HayeSep RCat. No.: 10304 (packing)Column: 10' x 1/8" stainless steel


Inj.: 15mL


1. Air2. Methane3. Carbon dioxide4. Ethylene5. Ethane6. Acetylene

0 2 64Min

5

6

4

3

2

1

1. Air2. Methane3. Carbon dioxide4. Ethane5. Propylene6. Propane7. Propadiene8. Methyl acetylene

0 2 4 6 7531Min

15

23

4

6

7

8

57

Figure 102. C1-C2 HydrocarbonsPacking: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 10' x 1/8" stainless steel


Det.: FID (3700 Varian)

Figure 101. Scott Mix 216, C1-C2, CO, and CO 2

Packing: HayeSep DCat. No.: 10293 (packing)Column: 20' x 1/8" Ni

Oven: 40°C (2 min) to 110°C at 24°C/minCarrier: helium, 30mL/min

Det.: P.E. 900 T.C., 225ma, 140°CInj.: 100mL, Valco valve, ambient


C1

-C2

Hydro

carb

ons/G

ases


1. Nitrogen (balance)2. Carbon monoxide, 1%3. Methane, 1%4. Carbon dioxide, 1%5. Acetylene, 1%6. Ethylene, 1%7. Ethane, 1%

0 2 4 6 108Min

2

1

3

4

5

6

7

4 02Min

4

23

1

1. Methane, 1%2. Acetylene, 1%3. Ethylene, 1%4. Ethane, 1%

58

Figure 103. Trace Acetylene in EthylenePacking: 45/60 Carboxen-1000Column: 5' x 1/8" stainless steelCat. No.: 12380 (general configuration stock

column; other stock columns available)Oven: 160°C

Carrier: helium, 30mL/minDet.: FIDInj.: 0.5mL ethylene with

trace impurities

Figure 104. Trace Acetylene in EthylenePacking: 60/80 Carbosieve S-IICat. No.: customColumn: 5' x 1/8" stainless steel


Det.: FIDInj.: 1mL, 100ppm acetylene

in ethylene

For more information,request Application Note 10.

795-0212


795-0213

Min0 18161412108642

1

2

3

1. Methane2. Acetylene3. Ethylene

Min1080 4

1

2

3

1. Methane2. Acetylene (14ppm)3. Ethylene

59

C1

-C3

Hydro

carb

ons

Min

0 2 4 6

76

5

4

3

2

1

Figure 106. C1-C3 HydrocarbonsPacking: 60/80 Carbosieve GCat. No.: 10198 (packing)Column: 5' x 1/8" stainless steel


Det.: FIDInj.: 1mL, appr. 15ppm each

component in nitrogen

For more information,request Bulletin 743 and Application Note 10.

795-0214

Figure 105. C1-C3 HydrocarbonsPacking: 45/60 Carboxen-1000Column: 2' x 1/8" stainless steelCat. No.: 12370-U (general configuration stock

column; other stock columns available)Oven: 165°C (1 min) to 220°C at 16°C/min

Carrier: helium, 60mL/minDet.: FIDInj.: 0.6mL Scott Gas Mix (Cat. No. 2-3470),

15ppm each component, appr. 10ngeach component on column

1. Methane2. Acetylene3. Ethylene4. Ethane5. Propyne6. Propylene7. Propane

For more information,request Bulletin 743 and Application Note 10.

795-0215

121086420

Min

1. Methane2. Acetylene3. Ethylene4. Ethane5. Propyne6. Propylene7. Propane

7

65

4

3

2

1

60

For more information, request Bulletin 743and Application Note 10.

795-0216

For more information, request Bulletin 743and Application Note 142.

713-0757

Figure 108. C3-C5 Saturated and Unsaturated HydrocarbonsPacking: 80/100 Carbopack C/0.19% picric acidCat. No.: 11824 (packing)Column: 2m x 1/8" stainless steelCat. No.: 13867 (general configuration stock column only)


Det.: FID

Figure 107. Methanol in Propylene/PropanePacking: 100/120 HayeSep TCat. No.: 10311 (packing)Column: 5' x 1/8" stainless steel


Det.: P.E. 900 T.C., 225ma, 150°CInj.: 132°C

1. Air, Methane2. Carbon dioxide3. Ethane4. Propylene, Propane5. Butane6. Butane7. Methanol

543210Min

76

5

2 3 4

1

1. Propane2. Propylene3. Isobutane4. 1-Butene5. n-Butane6. Isobutylene7. cis-Butene-28. trans-Butene-29. 1,3-Butadiene

10. Isopentane11. n-Pentane

Min

3

4

5

6

78

910

11

12106 14 168420

1,2

61

C1

-C5

Hydro

carb

ons

Figure 109. C3-C5 Saturated and Unsaturated HydrocarbonsPacking: 10% EDO-1 on 100/120 Chromosorb P AWCat. No.: 11967 (packing)Column: 20' x 1/8" stainless steel


Det.: FIDInj.: ASTM Hydrocarbon Mix

Section L, Blend 6

For more information, requestBulletin 743 and Application Note 142.

795-0217

21

3

4

5

6

7

8

9

1110

1. Propane2. Propylene3. Isobutane4. n-Butane5. 1-Butene6. Isobutylene7. trans-2-Butene8. Isopentane9. cis-2-Butene

10. n-Pentane11. 1,3-Butadiene

14121086420Min

Figure 110. C1-C5 Saturated and Unsaturated HydrocarbonsPacking: 15% EDO-1 on 60/80 Chromosorb P AWCat. No.: customColumn: 20' x 3/16" stainless steelCarrier: nitrogen, 40mL/min

For more information, request Bulletin 743 and Application Note 10 and 142.795-0218

11. Isopentane12. cis-2-Butene13. n-Pentane14. 3-Methyl-1-butene15. 1,3-Butadiene16. 1-Pentene17. 2-Methyl-1-butene18. trans-2-Pentene19. cis-2-Pentene20. 2-Methyl-2-butene

1. Methane2. Ethane3. Ethylene4. Propane5. Propylene6. Isobutane7. n-Butane8. 1-Butene9. Isobutylene

10. trans-2-Butene

0

132

4

5

6

7 8

9

10

11

12

13

14

15

16

19

201817

4 8 12 16 20 24 28 32 36 38Min

62

Figure 111. Trace C2-C6 HydrocarbonsPacking: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 10' x 1/8" stainless steel

Oven: 120°C to 200°C at 24°C/minCarrier: helium, 35mL/min


Min

Figure 112. C1-C5 HydrocarbonsPacking: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 10' x 1/8" stainless steel

Oven: 120°C to 200°C at 20°C/minCarrier: helium, 35mL/min




0123456789101112131415

6

54

1. Acetylene 16ppm2. Ethylene 15ppm3. Propylene 14.3ppm4. 1-Butene 15ppm5. 1-Pentene 14.75ppm6. 1-Hexane 16ppm

3

2

5 4 3 2 1 0Min

1. Methane 0.1894%2. Ethane 0.0965%3. Propane 0.0989%4. Isobutane 0.1019%5. Butane 0.1019%6. n-Pentane 0.2002%

6

5

4

3 2 1

7 689101112

1

63

2

C1

-C6

Hydro

carb

ons

For more information, request Bulletin 743, and Application Note 142.713-0758

Figure 113. C1-C6 Saturated and Unsaturated HydrocarbonsPacking: 23% SP-1700 on 80/100 Chromosorb P AWCat. No.: 11865 (packing)Column: 30' x 1/8" stainless steelCat. No.: 12809-U (general configuration stock column only)


Det.: FID, 150°CInj.: 0.6µL ASTM Section L, Blend 6,

plus C5 hydrocarbons, 100°C

Figure 114. Propane and Propyleneat High Concentrations

Packing: 23% SP-1700 on 80/100 Chromosorb P AWCat. No.: 11865 (packing)Column: 30' x 1/8" stainless steelCat. No.: 12809-U (general configuration stock column only)


Det.: FID, 150°CInj.: 0.5mL liquid propane gas, 100°C


1. Ethane2. Propane3. Propylene4. Isobutane5. n-Butane6. 1-Butene, Isobutylene7. trans-2-Butene8. cis-2-Butene9. Isopentane

10. 1,3-Butadiene11. Pentane12. 1-Pentene13. 2-Methyl-1-butene, trans-2-Pentene14. cis-2-Pentene15. 2-Methyl-2-butene16. 2-Methyl-1-pentane17. 3-Methyl-1-pentane18. n-HexaneU Unknown

19. 3-Methylhexane

0 2 10 12 144 16 18 20 22 24 26 28 30

Min

6 8

2

1 3

4 5 6 7

8

9

10

1213

14

15 17

16

18

U

19

11

0 12108642Min

6

8

5

7

4

3

1

1. Pressure disturbance2. Ethane 0.42%3. Propane 96.75%4. Propylene 1.72%5. Isobutane 0.93%6. n-Butane 0.17%7. 1-Butene, Isobutylene trace8. Isopentane trace

64

Figure 115. Industrial Butane IIIPacking: 23% SP-1700 on 80/100 Chromosorb P AWCat. No.: 11865 (packing)Column: 30' x 1/8" stainless steelCat. No.: 12809-U (general configuration

stock column only)Oven: 70°C

Carrier: helium, 25mL/minDet.: FID, 110°CInj.: 0.75mL industrial butane III


Figure 116. Permanent Gases in AirPacking: 60/80 Molecular Sieve 5ACat. No.: 20302 (packing)Column: 3' x 1/8" stainless steel


Det.: thermal conductivity, 150maInj.: 0.25cc, 1% concentrations of

H2, O

2, CH

4, and CO in N

2


1. Pressure disturbance2. Isobutane 2.02%3. n-Butane 97.92%4. Isopentane 0.05%5. n-Pentane 0.01%

0 2 4 6 8 10 1412 16Min

2 3

54

1

1. H2

2. O2

3. N24. CH45. CO

0 1 2 3 4 5 6Min

5

4

1

32

65

Perm

anent G

as/H

ydro

carb

ons

Figure 117. Permanent Gases in AirPacking: 60/80 Chromosorb 102Cat. No.: 20200-U (packing)Column: 6' x 1/8" stainless steel


Det.: thermal conductivity, 150maInj.: 0.2cc, 1% concentrations of

CH4, and CO

2 from N

2

Figure 118. Scott Mix 237Packing: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 20' x 1/8" Ni


Det.: P.E. 900 T.C, 225ma, 140°CInj.: 50mL vapor, Valco valve,

ambient

0

1. Nitrogen balance2. Oxygen 7%3. Carbon monoxide 7%4. Methane 4.5%5. Carbon dioxide 15%

1. H2, O2, N2, & CO2. CH43. CO24. H2O

1

4

32

1 2 3 4Min


1

4

5

3

2

Min

1 53 7 11 13 17159

795-0224

66

Figure 119. Nitrogen in ArgonPacking: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 30' x 1/8" stainless steel


Det.: P.E. 900 T.C, 300ma, 140°CInj.: 250mL Valco valve, 25°C

795-0226

Figure 120. Permanent GasesPacking: 80/100 HayeSep ACat. No.: 10283 (packing)Column: 36' x 1/8"


Det.: P.E. 900 T.C, 225maInj.: 25mL air plus hydrogen and carbon monoxide

0 1 2 3 4 5 6 7

1. Nitrogen 373ppm2. Argon balance

795-0225

1

2

0 1 2 3 4 5 6 7 8 9Min

1. Hydrogen 5%2. Nitrogen 48.5%3. Oxygen 13%4. Argon 0.5%5. Carbon monoxide 33%

2

5

3

1

4

67

Perm

anent G

as/H

ydro

carb

ons

Figure 121. Permanent Gases, Methane,and C2 Hydrocarbons

Packing: 100/120 Carbosieve S-IIColumn: 10' x 1/8" stainless steelCat. No.: 13821 (general configuration stock column;

other stock columns available)Oven: 35°C (7 min) to 225°C

at 32°C/minCarrier: helium, 30mL/min

Det.: thermal conductivity,150ma

Inj.: 0.6mL


1. Hydrogen 2.5%2. Oxygen 2.5%3. Nitrogen 91.4%4. Carbon monoxide 0.6%5. Methane 0.6%6. Carbon dioxide 0.6%7. Acetylene 0.6%8. Ethylene 0.6%9. Ethane 0.6%

Figure 122. Permanent Gases and C1-C2 HydrocarbonsPacking: 60/80 Carboxen-1000Column: 15' x 1/8" stainless steelCat. No.: 12390-U (general configuration stock column;


Carrier: helium, 30mL/minDet.: TCDInj.: 0.6mL Scott Gas Mix (Cat. No. 23437) with oxygen added, 1% each

component in nitrogen, on column


795-0228

80 4 1612 20 24Min

1

34

5

6 7 8

1. Oxygen2. Nitrogen3. Carbon monoxide4. Methane5. Carbon dioxide6. Acetylene7. Ethylene8. Ethane

2

0 2 4 6 8 10 12 14 1816 2220 24

Min

1

2

5

4

7

9

8

6

3

68

0

Figure 123. PesticidesPacking: GP 1.5% SP-2250/1.95% SP-2401 on 100/120 SUPELCOPORTCat. No.: 11947 (packing)Column: 6' x 4mm ID glass


Carrier: argon:methane (95:5), 70mL/minDet.: EC NI63Inj.: 5µL CP Mix


0 2 4 6 108 12 14 16 18Min

1

2

3

5

4

7

68

9

10

11

12

13

1. a -BHC2. g -BHC (Lindane)3. b -BHC4. Heptachlor5. Aldrin6. Heptachlor epoxide7. 4,4'-DDE8. Dieldrin9. 2,4-DDD (TDE)

10. Endrin11. 2,4'-DDT12. 4,4'-DDD (TDE)13. 4,4'-DDT


1. a -BHC2. g -BHC (Lindane)3. b -BHC4. Heptachlor5. Aldrin6. Heptachlor epoxide7. 4,4'-DDE8. 2,4-DDD (TDE)9. Dieldrin

10. 2,4'-DDT11. Endrin12. 4,4'-DDD (TDE)13. 4,4'-DDT

2 4 6 8 10 12 14 16 18 20Min

1

2 3

5

4

67

8

9

10 11

1213

Figure 124. PesticidesPacking: GP 4% SE-30/6% SP-2401 on 100/120 SUPELCOPORTCat. No.: 11948 (packing)Column: 6' x 4mm ID glass

Oven: 200°CCarrier: argon:methane (95:5), 70mL/min

Det.: EC NI63Inj.: 5µL CP Mix

69

Pestic

ides/P

henols

Figure 125. PhenolsPacking: 0.1% SP-1000 on 80/100 Carbopack CCat. No.: 11820 (packing)Column: 6' x 2mm ID glass (stock column available)


Det.: FIDInj.: 0.5µL approx. 0.1% each in MEK

Figure 126. Mixed PhenolsPacking: 3% SP-1000 on 100/120 SUPELCOPORTCat. No.: 11738 (packing)Column: 6' x 2mm ID glass


Det.: FID, 175°CInj.: 0.05µL

1. Phenol2. o-Cresol3. m-Cresol

p-Cresol4. 2,6-Dimethylphenol5. 2,4-Dimethylphenol

2,5-Dimethylphenol6. 3,5-Dimethylphenol7. 2,3-Dimethylphenol

m-Ethylphenolp-Ethylphenol

8. 3,4-Dimethylphenol9. 2,4,6-Trimethylphenol

10. 2,3,6-Trimethylphenol11. 2,3,5-Trimethylphenol12. 2,4,5-Trimethylphenol

0 2 64 108 12 14 16 18 20 11Min

1

2

3

4

5

6 7 8

9

1. Phenol2. o-Cresol3. m-Cresol4. p-Cresol5. o-Ethylphenol6. m-Ethylphenol7. p-Ethylphenol8. 2,6-Dimethylphenol9. 2,4-Dimethylphenol, 2,5-Dimethylphenol

10. 2,3-Dimethylphenol, 3,5-Dimethylphenol11. 3,4-Dimethylphenol

11

10



0 1 32 4 5 6 7 8 10 119 12Min

1

2

3

4

5

6 7

8

9

10

11 12

70

Figure 127. PhenolsPacking: 60/80 Carbopack F-TAColumn: 2m x 2mm ID glass


Det.: FIDInj.: 0.2µL approx. 0.1% each in MEK

Figure 128. Butyl PhenolsPacking: 10% SP-1000 on 80/100 SUPELCOPORTCat. No.: 11872 (packing)Column: 6' x 1/8" stainless steel


Det.: FIDInj.: 0.5µL with 0.1% of each

component in chloroform

795-0233


794-0139

20 4 6 8 10Min

11

9

8

76

5

2

3

4

1S

10S Solvent1. Phenol2. o-Cresol3. m-Cresol4. p-Cresol5. o-Ethylphenol6. p-Ethylphenol7. p-Ethylphenol8. 2,6-Dimethylphenol9. 2,4-Dimethylphenol

2,5-Dimethylphenol10. 2,3-Dimethylphenol

3,5-Dimethylphenol11. 3,4-Dimethylphenol

1. 2,6-Di-tert-butylphenol2. 2,6-Di-tert-butyl-p-cresol

0 1 2 3 4 5 6

MIn

2

1

71

20

Phenols

/Phero

mones

Figure 129. Phenols (Anisoles)Packing: 5% SP-1200/1.75% Bentone 34Cat. No.: 12134 (packing)Column: 6' x 1/8" stainless steelCat. No.: 12721 (general configuration stock column;


Carrier: nitrogen, 20mL/min

1. Anisole2. o-Methylanisole3. p-Methylanisole4. 2,6-Dimethylanisole5. m-Methylanisole6. 2,4-Dimethylanisole7. 2,3-Dimethylanisole8. 2,5-Dimethylanisole9. 3,4-Dimethylanisole

10. 3,5-Dimethylanisole

795-0238

Figure 130. PheromonesPacking: 15% SP-2340/ on 100/120 Chromosorb P AW-DMCSCat. No.: customColumn: 20' x 1/8" stainless steel

Oven: 225°CCarrier: nitrogen, 10mL/min at 50psi

Inj.: E and Z-9-tetradecenyl acetate

795-0234

0 1 2 3 54 76Min

1

2

3,4

56

87

9 10

Z

E

32

Min

2824

Z

72

Figure 131. SIMDIS Calibration Blend (ASTM D3710)Column: Petrocol™ A, 20" x 1/8" stainless steelCat. No.: 12445 (general configuration stock column only)

Oven: -20°C to 200°C at 20°C/minCarrier: helium, 25mL/min

Det.: FID, 300°CInj.: 0.1µL of D3710 Qualitative

Calibration Mix(Cat. No. 48884), 300°C

Figure 132. Gasoline by ASTM D3710Column: Petrocol A, 20" x 1/8"

stainless steelCat. No.: 12445 (general configuration

stock column only)Oven: -20°C to 250°C at 15°C/min

Carrier: helium, 25mL/minDet.: FID, 300°CInj.: 0.1µL gasoline, 300°C

1. C5s2. C6s3. C7s4. Toluene5. Xylenes

1. n-Propane2. Methylpropane3. n-Butane4. 2-Methylbutane5. n-Pentane6. 2-Methylpentane7. n-Hexane8. 2,4-Dimethylpentane9. n-Heptane

10. Toluene11. n-Octane12. p-Xylene13. n-Propylbenzene14. n-Decane15. n-Butylbenzene16. n-Dodecane17. n-Tridecane18. n-Tetradecane19. n-Pentadecane

0 1 2 3 4 5 6 7 8 9 10Min


1

2

3 4

5

78

6

9

10

11

12

13

14 16

17 1918

15

1086420

1

4

5

2

3

MinFor more information,request Bulletin 864.

713-0771

73

Figure 133. SIMDIS Calibration Blend (ASTM D2887)Column: Petrocol B, 20" x 1/8" stainless steelCat. No.: 12449 (general configuration stock column only)


Det.: FIDInj.: 0.1µL Quantitative Calibration Mix (Cat. No. 48882)

Figure 134. Reference Gas Oil Sample (ASTM D2887)Column: Petrocol B, 20" x 1/8" stainless steelCat. No.: 12449 (general configuration stock column only)


Det.: FIDInj.: 0.1µL Reference Gas Oil Sample (Cat. No. 48873).

Sim

ula

ted D

istilla

tion (S

IMD

IS)

1. nC62. nC73. nC84. nC95. nC106. nC117. nC128. nC149. nC16

10. nC1811. nC2012. nC2413. nC2814. nC3215. nC3616. nC4017. nC44


0 4 8 12 16 20

Min


0 2 4 6 8 10 1412 16 18 20 22 24Min

1

3

2

4

5 6 78

9

10

11 1213 14 15 16 17

74

Figure 135. PS-18-44D Wax Standard Mix (ASTM D2887)Column: Petrocol B, 20" x 1/8" stainless steelCat. No.: 12449 (general configuration stock column only)


Det.: FID, 380°CInj.: 1µL PS-18-44D Mix (Cat. No. 48928), 350°C

Figure 136. PS-18-60D Wax Standard Mix (ASTM D2887)Column: Petrocol B, 20" x 1/8" stainless steelCat. No.: 12449 (general configuration stock column only)


Det.: FID, 380°CInj.: 0.5µL PS-12-60D Mix (Cat. No. 48932), 350°C

1. n-Dodecane2. n-Tetradecane3. n-Hexadecane4. n-Octadecane5. n-Eicosane6. n-Docosane7. n-Tetracosane8. n-Hexacosane9. n-Octacosane

10. n-Triacontane11. n-Dotriacontane12. n-Hexatriacontane13. n-Tetracontane14. n-Tetratetracontane15. n-Pentacontane16. n-Hexacontane


1. n-Octadecane2. n-Eicosane3. n-Docosane4. n-Tetracosane5. n-Hexacosane6. n-Octacosane7. n-Triacontane8. n-Dotriacontane9. n-Hexatriacontane

10. n-Tetracontane11. n-Tetratetracontane

2 4 6 8 10 12 14 16 18 20 2422 26Min

1

2

3

4

5 7

6

8

9

10

11

794-0165

0 2 4 6 8 10 12 14Min

1

2

4

3

5

6

7

8

9

10

11

12

13

14

15

16

75

Figure 137. Crude Oil Qualitative Calibration StandardColumn: Petrocol C, 20" x 1/8" stainless steelCat. No.: 12455 (general configuration


Carrier: helium, 30mL/minDet.: FID, 360°CInj.: 1µL Crude Oil Qualitative

Calibration Standard, 360°C

Figure 138. Crude Oil — North Sea, OsebergColumn: Petrocol C, 20" x 1/8"

stainless steelCat. No.: 12445 (general configuration


Carrier: helium, 30mL/minDet.: FID, 360°CInj.: 1µL oil:CS mix (1:1), 360°C

Sim

ula

ted D

istilla

tion (S

IMD

IS)For more information, request Bulletin 864.

711-0072

0 4 8 12 16 20 24 28Min

4

5

6

3

1

7

8 9

1718

1920

12131415

16

2

2122

23

For more information, request Bulletin 864.711-0076,0077

Min

00

1. Porpane (nC3), 1.0%2. n-Butane (nC4)3. n-Pentane (nC5), 3.4%4. n-Hexane (nC6), 5.2%5. n-Heptane (nC7)6. n-Octane (nC8), 6.2%7. n-Nonane (nC9), 4.7%8. n-Decane (nC10)9. n-Undecane (nC11)

10. n-Dodecane (nC12)11. n-Tridecane (nC13)12. n-Tetradecane (nC14)13. n-Pentadecane (nC15)14. n-Hexadecane (nC16)15. n-Heptadecane (nC17)16. n-Octadecane (nC18)17. n-Eicosane (nC20)18. n-Tetracosane (nC24)19. n-Octacosane (nC28)20. n-Dotriacontane (nC32)21. n-Hexatriacontane (nC36)22. n-Tetracontane (nC40)23. n-Tetratetracontane (nC44)

4.4% each by weight, except as noted.

40 8 12 16 20 24

Without Internal Standard

With Internal Standard

1. Tetradecane (nC14)2. Pentadecane (nC15)3. Hexadecane (nC16)4. Heptadecane (nC17)

1 32

4

1011

76

Figure 139. SIMDIS, Regular GasolinePacking: 10% SP-2100 on 100/120 SUPELCOPORTCat. No.: 12140 (packing)Column: 10' x 1/8" stainless steelCat. No. 13766 (general configuration stock column;


Carrier: 20mL/minDet.: FIDInj.: 0.1µL

Figure 140. NaphthaPacking: 10% SP-2100 on 100/120 SUPELCOPORTCat. No.: 12140Column: 10' x 1/8" stainless steelCat. No.: 13766 (general configuration stock column;




711-0088

1. C62. C83. C104. C125. C146. C16


MIn0 2 4 6 8 10 12

1 2 43

1. C62. C83. C104. C12

1 2 3 4 5 6

20 4 6 8 10 12 14 16 18

MIn

Fraction

Fraction

77

Figure 142. Microcrystalline WaxPacking: 1% Dexsil ® 300 on 100/120 SUPELCOPORTCat. No.: 11972 (packing, 20g/bottle)Column: 18' x 1/8" stainless steel


Det.: 350°CInj.: 1µL chloroform containing 30µg wax (325°C)

Figure 141. Kerosene (Jet A)Packing: 10% SP-2100 on 100/120 SUPELCOPORTCat. No.: 12140 (packing)Cat. No.: 13766 (general configuration stock column;

other stock columns available)Column: 10' x 1/8" stainless steel



Perm

anent G

as/H

ydro

carb

ons


0 20

MIn

242218161412108642


MIn

0 2 4 6 8 10 12 14 16 18 20 22 24

1 2 3 4 5 6

1. C62. C83. C104. C125. C146. C16

Fraction

78

Figure 143. WaxPacking: 3% Dexsil ® 300 on 100/120 SUPELCOPORTCat. No.: 11973 (packing)Column: 6' x 1/8" stainless steel


Figure 144. Solvents — Alcohol Retarder ColumnPacking: 15% THEED on 100/120 Chromosorb W AWCat. No.: 11823 (packing)Column: 10' x 1/8" stainless steel

Oven: 80°CCarrier: nitro

Det.: FIDInj.: 0.5µLgen, 20mL/min



MIn

1. C102. C123. C144. C165. C186. C207. C228. C249. C26

10. C2811. C3012. C3213. C3414. C3615. C3816. C4017. C44

1 32 4

56

78

910

1112

1314

1516

17

0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60

0

MIn

1 2 3 4 5 6 7 8 9

1. Heptane2. Octane3. Ethyl acetate4. n-Propyl acetate5. Toluene6. Ethyl benzene7. Xylene8. Isopropyl alcohol9. Ethyl alcohol

10. n-Propyl alcohol

1

2

3 4

5

78 9

610

Fraction

79

Figure 145. SolventsPacking: 60/80 Carbowax C/0.2% Carbowax 1500Cat. No.: 11826 (packing)Column: 6' x 1/8" stainless steelCat. No.: 13860-U (general configuration stock column,



Figure 146. Solvents (Methanol through Pentanol)Packing: GP 80/100 Carbopack C/0.2% Carbowax 1500Cat. No.: 11826 (packing)Column: 6' x 2mm stainless steelCat. No.: 13860-U (general configuration stock column;


Carrier: nitrogen, 20mL/min, 31psiDet.: FIDInj.: 0.02µL


1. Methanol2. Ethanol3. 2-Propanol4. 1-Propanol5. 2-Methyl-2-propanol (1-butyl)6. 2-Butanol (sec-butyl)7. 2-Methyl-1-propanol (iso)8. 1-Butanol9. 2-Methyl-2-butanol (t-amyl)


For more information,request Bulletins 816 and 824.

795-0244

0Min

1 2 3 4 5 6 7 8

1

2

4

5

6

7 8

9

10 1211

14

13

1615

3

0 1 2 3 5 6 7 8

9

7

85

6

4

321

1. Methanol2. Ethanol3. Acetone4. Isopropyl alcohol5. Ethyl ether6. Ethyl acetate7. Methyl ethyl ketone8. Isobutyl alcohol9. Benzene

4

Solv

ents

80

Figure 147. Solvents (Ethyl Ether Through Styrene)Packing: 10% SP-1000 on 80/100 SUPELCOPORTCat. No.: 11872 (packing)Column: 20' x 1/8" stainless steelCat. No.: 12719 (general configuration stock column;


Carrier: nitrogen, 30mL/minDet.: FIDInj.: 5µL CS

2 approx.

100ppm each solvent

Figure 148. Solvents (Methanol Through Xylenes)Packing: 20% SP-2100/0.1% Carbowax 1500 on 100/120 SUPELCOPORTCat. No.: 11821 (packing)Column: 10' x 1/8" stainless steelCat. No.: 12718 (general configuration stock column;


Carrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL, approx. equal proportions

of neat compounds

795-0240

1. Methanol2. Ethanol3. Acetone4. Methyl ethyl ketone5. Ethyl acetate6. Isobutyl alcohol7. Methyl Cellosolve8. Isopropyl acetate9. 2-Nitropropane

10. MIBK11. Isobutyl acetate12. Toluene13. n-Butyl acetate14. Diacetone alcohol15. Ethylbenzene16. m- & p-Xylene17. Cellosolve acetate18. Butyl Cellosolve & o-Xylene

0 1 2 3 54 6Min

7 8 109 11 12 13

1

2

3

4

5

6

7

8

9 10 11

12

1314 16

1718

15


30 1 8765Min

8

9

10

11

12

13

1418

17 19

20

3

4

1

2

65

7

15

16

For more information, request Bulletins 816 and 824.

1. Ethyl ether2. Isooctane3. Carbon disulfide4. Acetone5. Carbon tetrachloride,

Methylchloroform6. Methyl ethyl ketone7. Methylene chloride8. Benzene9. Trichloroethylene

10. Chloroform11. Tetrachloroethylene12. Toluene13. Ethylene dichloride14. Dioxane15. Ethylbenzene16. p-Xylene17. m-Xylene18. o-Xylene19. 1,1,2-Trichloroethane20. Styrene

8 1

Figure 149. Solvents — Ketone-Retarding ColumnPacking: 20% SP-2401/0.1% Carbowax 1500 on 100/120 SUPELCOPORTCat. No.: 11822 (packing)Column: 10' x 1/8" stainless steel


Det.: FIDInj.: 0.1µL, approx. equal proportions

of neat components

Figure 150. Industrial SolventsPacking: 80/120 Carbopack B/3% SP-1500Cat. No.: 11813-U (packing)Column: 10' x 1/8" stainless steelCat. No.: 12592 (general configuration stock column;


Solv

ents

0 4

1

2

34

6

7,8

5

9

10

11

12

18

16

19

20

21

22

Impu

rity

17

23,24,25

26

27 28

29

30

3231

16128 16 16 16 16 16 16 16Min

713-0787

For more information,request Bulletins 816 and 824.

795-0245

0 1 2 3 4 5 6 7 8Min

2

13 5

4

67

8

1. Isopropyl alcohol2. Ethyl acetate3. Methyl ethyl ketone4. Toluene5. n-Propyl acetate6. Isobutyl acetate7. MIBK8. Mesityl oxide


1. Methanol2. Methyl formate3. Ethanol4. Acetone5. Methylene chloride6. Isopropyl alcohol7. Ethyl formate8. Methyl acetate9. n-Propyl alcohol

10. Tetrahydrofuran (THF)11. Methyl ethyl ketone

(MEK)12. Methyl Cellosolve13. sec-Butyl alcohol14. Ethyl acetate15. Isobutyl alcohol16. 2-Nitropropane17. n-Butyl alcohol18. Ethyl Cellosolve19. n-Propyl acetate20. sec-Butyl acetate21. Cyclohexanone22. Isobutyl acetate23. n-Butyl acetate24. Toluene25. Mesityl oxide26. Cellosolve acetate27. Butyl Cellosolve28. Isoamyl acetate29. Ethylbenzene30. m-Xylene31. p-Xylene32. o-Xylene

151314

8 2

Figure 151. Industrial SolventsPacking: 60/80 Carbopack B/1% SP-1510Cat. No.: 11809 (packing)Column: 10' x 1/8" stainless steel


Det.: FIDInj.: 0.3µL, approx. equal proportions of neat components

1. Methanol2. Methyl formate3. Ethanol4. Methylene chloride5. Acetone6. Isopropyl alcohol7. Ethyl formate8. Methyl acetate9. n-Propyl alcohol

10. Tetrahydrofuran (THF)

21. sec-Butyl acetate22. Isobutyl acetate23. n-Butyl acetate24. Mesityl oxide25. Toluene26. Cellosolve acetate27. Butyl Cellosolve28. Isoamyl acetate29. Ethylbenzene30. m-Xylene31. o-Xylene32. p-Xylene

11. Methyl ethyl ketone (MEK)12. Methyl Cellosolve13. sec-Butyl alcohol14. Isobutyl alcohol15. Ethyl acetate16. 2-Nitropropane17. n-Butyl alcohol18. Ethyl Cellosolve19. n-Propyl acetate20. Cyclohexanone


0 2 6 8 10 14 22 24 28 30 32 34Min

4 12 1816 20 26 36

1

3

2 4

5 6

10

1113

7

8,9

12

14

1615

17

18

20

19

21

22

25

26,27

2930

31,32

Impurity

2324

28

Figure 152. Industrial SolventsPacking: 80/100 Carbopack C/0.1% SP-1000Cat. No.: 11820 (packing)Column: 10' x 1/8" stainless steel


Det.: FIDInj.: 0.3µL, 3% each component by

volume (approx. 12µg each)


1. Methanol2. Methyl formate3. Ethanol4. Acetone5. Methylene chloride6. Isopropyl alcohol7. Ethyl formate8. Methyl acetate9. Tetrahydrofuran (THF)

10. n-Propyl alcohol11. Methyl ethyl ketone (MEK)12. Methyl Cellosolve13. sec-Butyl alcohol

14. Isobutyl alcohol15. Ethyl acetate16. 2-Nitropropane17. n-Butyl alcohol18. Ethyl Cellosolve19. n-Propyl acetate20. Cyclohexanone21. sec-Butyl acetate22. Isobutyl acetate23. n-Butyl acetate24. Toluene25. Mesityl oxide26. Cellosolve acetate27. Butyl Cellosolve28. Isoamyl acetate29. Ethylbenzene30. m-Xylene31. p-Xylene32. o-Xylene

20 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44 46Min

2

3

4

56 9

10

1

1117

1819

20

21

22 23

24,25

2726

29

30

31,3212

28Impurity

1615

1314

7,8

8 3

Solv

ents

/Alc

ohols

Figure 153. Industrial SolventsPacking: 60/80 Carbopack F-TAColumn: 2m x 2mm ID glass

Oven: 75°C (2 min) to 225°Cat 4°C/min

Carrier: nitrogen, 20mL/minDet.: FIDInj.: 0.05µL solvents mix,

3% each solvent

Figure 154. Alcohols, C1-C5Packing: 80/100 Carbopack F-SLColumn: 2m x 2mm ID TightSpec glass




0Min

12. Methyl Cellosolve13. sec-Butyl alcohol14. Ethyl acetate15. Isobutyl alcohol16. 2-Nitropropane17. n-Butyl alcohol

I Impurity18. Ethyl Cellosolve19. n-Propyl acetate20. Cyclohexanone21. sec-Butyl acetate22. Isobutyl acetate23. n-Butyl acetate24. Toluene25. Mesityl oxide26. Cellosolve acetate27. Butyl Cellosolve28. Isoamyl acetate29. Ethylbenzene30. m-Xylene31. p-Xylene32. o-Xylene

1. Methanol2. Methyl formate3. Ethanol4. Acetone5. Methylene chloride6. Isopropyl alcohol7. Ethyl formate8. Methyl acetate9. n-Propyl alcohol

10. Tetrahydrofuran(THF)

11. Methyl ethylketone (MEK)

2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32

1

3

4

6

9

10

5

7,82 12

1311

14,15

17

18 19

20

21

22I

23

24

28,29 30

16 I

25

0 1 2 3 4 5Min

1

2

34

7

5

9

8

6

10

11

12

13

14

1516

1. Methanol2. Ethanol3. i-Propanol4. n-Propanol5. t-Butanol6. i-Butanol7. 2-Methyl-1-propanol8. n-Butanol9. 2-Methyl-2-butanol

10. 2,2-Dimethyl-1-propanol11. 3-Methyl-2-butanol12. 3-Pentanol13. 2-Pentanol14. 2-Methyl-1-butanol15. 3-Methyl-1-butanol16. n-Pentanol

31,32

26,27

8 4

Figure 155. SugarsPacking: 3% SP-2330 on 100/120 SUPELCOPORTCat. No.: 11802 (packing)Column: 6' x 2mm ID glass


Det.: FIDInj.: 5µL

Figure 156. SugarsPacking: 3% SP-2340 on 100/120 SUPELCOPORTCat. No.: 11863 (packing)Column: 6' x 2mm ID glass


Det.: FIDInj.: 4µL

795-0247

0 2 4 6 8 10 12Min

14 16 18 20 22 24

1

2

3

4

5

6

7

8 9 1110

1312

1. Glyceraldehyde2. Erythrose3. Deoxyribose4. Rhamnose5. Fucose6. Ribose7. Arabinose8. Xylose9. Deoxyglucose

10. Mannose11. Galactose12. Glucose13. Inositol

795-0249

1. Glyceraldehyde2. Erythrose3. Deoxyribose4. Rhamnose5. Fucose6. Ribose7. Arabinose8. Xylose9. Deoxyglucose

10. Mannose11. Galactose12. Glucose13. Inositol

20 4 6 8 10 12 14 16 2018Min

1312

10119

7

8

6

5

3,4

2

1

8 5

Sugars

/Sulfu

r & M

erc

apta

ns

Figure 157. Sulfur Dioxide in NitrogenPacking: 45/60 Carboxen-1000Column: 2' x 1/8" stainless steelCat. No.: 12370-U (general configuration stock column;


Carrier: helium, 30mL/minDet.: TCDInj.: 1µL of 1000ppm SO

2 in N

2,

approx. 1µg SO2 on column

Figure 158. Sulfur Gases and HydrocarbonsPacking: HayeSep QCat. No.: 10301-U (packing)Column: 8' x 1/8"




2

1

1 20Min

1. Nitrogen2. Sulfur dioxide

1. Air2. Methane3. Carbon dioxide4. Ethylene5. Acetylene6. Ethane7. Hydrogen sulfide8. Carbonyl sulfide9. Propylene

10. Propane11. Propadiene

2 4 6Min

0

9

10

11

8

7

6

3

54

21

795-0251

8 6

Figure 159. Hydrogen Sulfide, Carbon Dioxide, and WaterPacking: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 10' x 1/8" Ni


Det.: P.E. 900 T.C., 225ma, 140°CInj.: Valco valve, 50 microliters

vapor (ambient), 100°C

Figure 160. Hydrogen Sulfide, Nitrogen, Carbon Dioxide,Nitrous Oxide and Water

Packing: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 10' x 1/8" stainless steel


Det.: P.E. 900 T.C., 225maInj.: Valco valve, 100 microliters, 140°C


1. Air (balance)2. Carbon dioxide (approx. 0.1%)3. Water (approx. 2.5%)4. Hydrogen sulfide (approx. 2.5%)

0 2 4 86

Min

4

3

2

1


0 2 4Min

12

3

5

4

1. Nitrogen (balance)2. Carbon dioxide (2%)3. Nitrous oxide (3%)4. Water (0.5%)5. Hydrogen sulfide (3%)

8 7

Sulfu

r & M

erc

apta

ns

Figure 162. Trace Sulfur GasesPacking: 40/60 Carbopack B HT 100Cat. No.: 20272 (packing)Column: 1.4m x 1/8" OD Teflon (FEP)Cat. No.: 11502-U (general configuration


Carrier: nitrogen, 20mL/minDet.: flame photometricInj.: 0.3mL

Figure 161. Light Sulfur Gases, Alkyl Sulfides,and Mercaptans

Packing: 40/60Carbopack B HT 100Cat. No.: 20272 (packing)Column: 1.4m x 1/8" OD Teflon ® (FEP)Cat. No.: 11502-U (general configuration stock column only)


Det.: flame photometricInj.: 0.5mL of synthetic

mix

1. H2S2. COS3. SO24. CH2SH

20

Min

3

1

4

2



20 6 84 10 12Min

12

3

4

6

5

78 9

1. SF6, H2S2. SO2, COS3. CH3SH4. Dimethyl sulfide, Ethyl mercaptan5. CS26. Ethyl methyl sulfide, n-Propyl mercaptan7. Methyl disulfide8. Diethyl sulfide9. n-Butyl mercaptan

8 8

8

0

Figure 163. Kraft Pulp Mill Stack GasesPacking: Supelpak™-SColumn: 30" (18" packed) x 1/8" OD

Teflon ® (FEP)Cat. No.: 12255-U (general configuration

stock column only)Oven: 30°C (1 min) to 210°C at 40°C/min

Carrier: helium, 30mL/minDet.: flame photometricInj.: 2mL


1. H2S2. COS3. SO24. CH3SH5. (CH3)2S6. (CH3)2S2

0 2 4 6Min

6

5

4

3

2

1


713-0805

1. H2S2. SF63. COS4. SO25. Methyl mercaptan6. Ethyl mercaptan7. Dimethyl sulfide8. CS2

2 4 6 8 10Min

1

2

3

4

5

7

6

Figure 164. Trace Sulfur Gases with C 2 IsomericSulfur Compounds

Packing: GP 60/80 Carbopack B/1.5% XE-60/1.0% H 3PO4Cat. No.: 11828 (packing)Column: 6' x 2mm ID glass


Det.: flame photometricInj.: 0.25mL

8 9

Sulfu

r & M

erc

apta

ns

Figure 165. Alkyl Sulfides and MercaptansPacking: Chromosil 330Column: 8' (6' packed) x 1/8" OD Teflon (FEP)Cat. No.: 11496 (general configuration stock column only)


Det.: flame photometricInj.: 0.5cc of synthetic mix

Figure 166. Trace Light Sulfur Gases and C1-C3 MercaptansPacking: Chromosil 330Column: 8' (6' packed) x 1/8" OD Teflon (FEP)Cat. No.: 11496 (general configuration stock column only)


Det.: flame photometricInj.: 0.2mL, approx. 1ppm

each component in nitrogen



713-0804

1. COS2. H2S3. Methyl mercaptan4. CS25. Ethyl mercaptan6. SO27. Propyl mercaptan

20 4 0 0

Min

1

2

3

4

5

67

Min

9

7

8

6

4

2

3

0 2 4 6 8 10

1. COS + H2S2. CH3SH3. CS24. SO25. Ethyl mercaptan, Dimethyl sulfide6. Propyl mercaptan, Ethyl methyl sulfide7. Diethyl sulfide8. Butyl mercaptan9. Methyl disulfide

1 5

9 0

Figure 167. Light Sulfur Gases at Percent ConcentrationsPacking: Chromosil 310Column: 6' x 4mm ID glassCat. No.: 11501 (general configuration


Carrier: helium, 50mL/minDet.: thermal conductivityInj.: 0.3mL of synthetic mix


Figure 168. Trace Light Sulfur GasesPacking: Chromosil 310Column: 8' (6' packed) x 1/8" OD Teflon (FEP)Cat. No.: 11501 (general configuration stock column only)


Det.: flame photometricInj.: 0.5mL approx. 1ppm each

component in nitrogen

Min


0 42 6Min

4

3

2

1

1. COS2. H2S3. CS24. SO2

0 2 4 6

5

6

4

2

31

1. Air2. CO23. COS4. H2S5. CS26. SO2

9 1

Sulfu

r & M

erc

apta

ns


Figure 169. Mercaptans, Sulfides, and DisulfidesPacking: 12% polyphenyl ether/0.5% H 3PO4 on 40/60 Chromosorb TColumn: 36' x 0.085" ID TeflonCat. No.: 11500 (general configuration stock column only)


Det.: FPDInj.: 0.5cc synthetic mix

Figure 170. Sulfur GasesPacking: 12% polyphenyl ether/0.5% H 3PO4 on 40/60 Chromosorb TColumn: 36' x 0.085" ID TeflonCat. No.: 11500 (general configuration stock column only)


Det.: flame photometric (sens. settings on figure)Inj.: 0.2mL nitrogen approx. 1ppm each analyte


0 2 4 6 8 10 12 2418 26Min

1

34

2

5

6

7 9 108

1. SF62. H2S & COS3. SO24. Methyl mercaptan5. Ethyl mercaptan6. Dimethyl sulfide & CS27. n-Propyl mercaptan8. Methyl ethyl sulfide9. iso-Butyl mercaptan

10. n-Butyl mercaptan & Diethyl sulfide

0 1 2 3 4 65

2 x 10-6 4 x 10-8 2 x 10-81 x 10-6

23

4

5

6

1

1. Air2. H2S3. SO24. CH3SH5. C2H5SH6. (CH3)2S

9 2

Figure 171. Cholesterol (Steroids)Packing: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11878 (packing)Column: 3' x 2mm ID glass





795-0235

1. 5a -Cholestane2. Cholesterol3. 5a -Cholestane-3b -ol4. 5a -Cholestane-3-one

795-0236

1. 5a -Cholestane2. Cholesterol3. 5a -Cholestane-3b -ol4. 5a -Cholestane-3-one

0 1 2 43 5 6 7 8 109

1 42,3

Min

0 1 2 3 4 5 6 7 8 9 10

3

4

12

Min

9 3






Ste

roid

s

1. 5 a -Cholestane2. Cholesterol3. 5 a -Cholestane-3b -ol4. 5 a -Cholestane-3-one

795-0237

1. 5 a -Cholestane2. Cholesterol3. 5 a -Cholestane-3b -ol4. 5 a -Cholestane-3-one

795-0283

1

3

2

4

0 1 2 3 4 5 6 7 8Min

Min10 1312111098765432

3

1

4

2

9 4

795-0257

795-0258

1. n-Octacosane2. Androsterone TMS3. Etiocholanolone TMS4. Dehydroepiandrosterone TMS5. 11-Ketoetiocholanolone TMS6. n-Hexatriacontane7. 11b -OH-Etiocholanolone TMS

Figure 176. KetosteroidsPacking: 3% OV-225 on 80/100 SUPELCOPORTCat. No.: 11957-U (packing)Column: 6' x 4mm ID glass


Figure 175. Plant SterolsPacking: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11878 (packing)Column: 3' x 2mm ID glass


0 1 2 3 4 5 6 7 8 9 1110 12Min

765

43

2

1

1. Brassicosterol2. Compesterol3. Stigmasterol4. b -Sitosterol

0 4 6 82 10 12 14 16

Min

4

2

1

3

9 5

Ste

rols

/Trig

lycerid

es

Figure 177. TriglyceridesPacking: 1% Dexsil 300 on 100/120 SUPELCOPORTCat. No.: 11972 (packing, 20g/bottle)Column: 18" x 1/8" stainless steel


Det.: FID, 350°CInj.: 1µL chloroform containing 1µg each triglyceride, 325°C

Figure 178 Pentaerithritol EstersPacking: 1% Dexsil 300 on 100/120 SUPELCOPORTCat. No.: 11972 (packing)Column: 18" x 1/8" stainless steel


Det.: 350°C

713-0944

0 2 4 6 8 10 12

Min

2

1

3

4

1. Trilaurin2. Trimyristin3. Tripalmitin4. Tristearin

795-0259

0 2 4 6 8 10 12 14 16 18 20

Min

C34 to C48 Alcohols

9 6

Figure 179. Cholesteryl EstersPacking: 1% Dexsil 300 on 100/120 SUPELCOPORTCat. No.: 11972 (packing)Column: 18" x 2mm ID glass


Det.: 350°C

713-0943For expert answers to your questions,

contact our Technical Service DepartmentPhone 800-359-3041 or 814-359-3041FAX 800-359-3044 or 814-359-5468

0 1 2 3 4 5 6 7Min

2

1

34

1. Laurate2. Myristate3. Palmitate4. Stearate

AYT

For more information, or current prices, contact your nearest Supelco subsidiary listed below. Toobtain further contact information, visit our website (www.sigma-aldrich.com), see the Supelcocatalog, or contact Supelco, Bellefonte, PA 16823-0048 USA.ARGENTINA · Sigma-Aldrich de Argentina, S.A. · Buenos Aires 1119AUSTRALIA · Sigma-Aldrich Pty. Ltd. · Castle Hill NSW 2154AUSTRIA · Sigma-Aldrich Handels GmbH · A-1110 WienBELGIUM · Sigma-Aldrich N.V./S.A. · B-2880 BornemBRAZIL · Sigma-Aldrich Quimica Brasil Ltda. · 01239-010 São Paulo, SPCANADA · Sigma-Aldrich Canada, Ltd. · 2149 Winston Park Dr., Oakville, ON L6H 6J8CZECH REPUBLIC · Sigma-Aldrich s.r.o.· 186 00 Praha 8DENMARK · Sigma-Aldrich Denmark A/S · DK-2665 Vallensbaek StrandFINLAND · Sigma-Aldrich Finland/YA-Kemia Oy · FIN-00700 HelsinkiFRANCE · Sigma-Aldrich Chimie · 38297 Saint-Quentin-Fallavier CedexGERMANY · Sigma-Aldrich Chemie GmbH · D-82041 DeisenhofenGREECE · Sigma-Aldrich (o.m.) Ltd. · Ilioupoli 16346, AthensHUNGARY · Sigma-Aldrich Kft. · H-1067 BudapestINDIA · Sigma-Aldrich Co. · Bangalore 560 048IRELAND · Sigma-Aldrich Ireland Ltd. · Dublin 24ISRAEL · Sigma Israel Chemicals Ltd. · Rehovot 76100ITALY · Sigma-Aldrich s.r.l. · 20151 MilanoJAPAN · Sigma-Aldrich Japan K.K. · Chuo-ku, Tokyo 103KOREA · Sigma-Aldrich Korea · SeoulMALAYSIA · Sigma-Aldrich (M) Sdn. Bhd. · 58200 Kuala LumpurMEXICO · Sigma-Aldrich Química S.A. de C.V. · 50200 TolucaNETHERLANDS · Sigma-Aldrich Chemie BV · 3330 AA ZwijndrechtNORWAY · Sigma-Aldrich Norway · Torshov · N-0401 OsloPOLAND · Sigma-Aldrich Sp. z o.o. · 61-663 PoznañPORTUGAL· Sigma-Aldrich Quimica, S.A. · Sintra 2710RUSSIA · Sigma-Aldrich Russia · Moscow 103062SINGAPORE · Sigma-Aldrich Pte. Ltd.SOUTH AFRICA · Sigma-Aldrich (pty) Ltd. · Jet Park 1459SPAIN · Sigma-Aldrich Quimica, S.A. · 28100 Alcobendas, MadridSWEDEN · Sigma-Aldrich Sweden AB · 135 70 StockholmSWITZERLAND · Supelco · CH-9471 BuchsUNITED KINGDOM · Sigma-Aldrich Company Ltd. · Poole, Dorset BH12 4QHUNITED STATES · Supelco · Supelco Park · Bellefonte, PA 16823-0048 · Phone 800-247-6628 or814-359-3441 · Fax 800-447-3044 or 814-359-3044 · email:[email protected] H

Supelco is a member of the Sigma-Aldrich family. Supelco products are sold through Sigma-Aldrich,Inc. Sigma-Aldrich warrants that its products conform to the information contained in this and otherSigma-Aldrich publications. Purchaser must determine the suitability of the product for a particular use.Additional terms and conditions may apply. Please see the reverse side of the invoice or packing slip.

Supelco Park, Bellefonte, PA,16823 USA

To Order:Phone 800-247-6628 or 814-359-3441FAX 800-447-3044 or 814-359-3044

packed column gc application guide

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