Patterns of gene expression

Drosophila embryogenesis

in

RNA insitu and imaging pipeline

Richard Weiszmann

96-well plate RNA insitu

DGC1 and DGC2

Elaine Kwan

production snapshots

Amy Beaton

imaging and annotation

Status report

- to date we captured 25000 high resolution images

- we examined approximately 2500 genes

- images were captured for over 1200 genes

Correlation of microarray and image data

Novel insitu pattern – is it real ?

AnnotationCollaboration with Volker Hartenstein

differentiated organ

malpighian tubules

tracheal system

oenocytes fat body

anlage in statu nascendi

dorsal ectoderm mesoderm

procephalic ectoderm hindgut

primordium

malpighian tubules

posterior midgut

anlage

head mesoderm

large intestine

anlagein statu nascendi

anlage primordium differentiatedorgan

~ ~ ~

stage4-6

mesectodermanlagein statu nascendi

~

stage7-8

mesectodermanlage

~

stage9-12

midline primordium

~

Tracing back single-minded

stage13-16

midline glia

- allows integration with existing gene expression data

- annotation terms can be easily expanded for greater detail

Gene ontology

-Flybase controlled vocabulary for anatomy

(collaboration with Michael Ashburner).

- allows sophisticated searches of the dataset

http://www.fruitfly.org

Coming

soon

to

collaboration with Suzanna Lewis and Shu ShengQiang

Three adjacent Brachyury/T-Box genes at 66E4

CG7312 - earmuff (encodes a Zinc_Finger_C2H2, EF_hand protein)

Drosophila

Zebrafish XenopusYang, Z. et.al. Matsuo-Takasaki, M. et.al.

Acknowledgements

Sue CelnikerRichard Weiszmann

Amy BeatonElaine Kwan

Volker Hartenstein

Michael AshburnerSuzanna LewisShu ShengQiang

Gerry Rubin