Supporting information

Platinum nanoparticles/graphene oxide hybrid with excellent

peroxidase-like activity and its application for cysteine detection

Xiao-Qing Lin,a,b Hao-Hua Deng,a,b Gang-Wei Wu,a,c Hua-Ping Peng,a,b Ai-Lin Liu,a,b

Xin-Hua Lin,a,b Xing-Hua Xia,d Wei Chen*a,b

a Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004,

China

b Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou

350004, China

c Department of Pharmacy, Fujian Provincial Hospital, Fuzhou 350001, China

d State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry

and Chemical Engineering, Nanjing University, Nanjing 210093, China.

* Corresponding author. Tel./fax: +86 591 22862016.

E-mail address: chenandhu@163.com (W. Chen).

Xiao-Qing Lin and Hao-Hua Deng contributed equally to this work.

Electronic Supplementary Material (ESI) for Analyst.This journal is © The Royal Society of Chemistry 2015

Fig. S1 Photographs of bare PtNPs (A, C) and PtNPs/GO (B, D). A, B: freshly

prepared; C, D: placed for 120 h.

Fig. S2 UV absorption spectra of PtNPs (a) and PtNPs/GO (b).

Fig. S3 (A-D) Dependency of the relative activity of PtNPs/GO on (A) pH, (B)

temperature, (C) concentration of H2O2, and (D) concentration of TMB. (E-F)

Relationship between A652 with (E) reaction time and (F) concentration of PtNPs/GO.

Fig. S4 Catalytic activity of PtNPs/GO in different TMB concentrations.

Fig. S5 Comparison of the catalytic activity of PtNPs/GO (a) and porous PtNPs/GO

(b) with the same amount of platinum (A) and GO (B).

Fig. S6 Dependency of catalytic activity of PtNPs/GO (a) and porous PtNPs/GO (b)

on H2O2. The concentration of TMB for PtNPs/GO and porous PtNPs/GO was 124.35

μM and 829 μM, respectively.

Fig. S7 Selectivity of the sensor toward Cys (2000 nM) over other biomolecules

(2000 nM). Samples marked with 1-18 corresponding to L-serine, L-isoleucine, L-

leucine, L-alanine, L-phenylalanine, L-tyrptophan, L-aminoacetic acid, L-valine, L-

proline, L-threonine, L-arginine, L-histidine, L-aspartic acid, glucose, L-methionine,

ascorbic acid, glutathione and Cys, respectively.

Table S1 Comparison of various analytical methods for Cys determination

MaterialsLinear range

(nM)LOD (nM) Reference

Copper@gold nanoparticles 0-1500 50 1

Di-N-methyl-N-hydroxyethylaniline

Squaraine 10-700 3.9 2

Gold nanoparticles 166-1670 100 3

Gold nanoparticles 0-8000 20 4

Graphene nanoribbon 25-500 - 5

Silver nanoclusters 25 -6000 20 6

DNA-gold nanoparticles 50-10000 100 7

DNA/ligand/ion ensemble 2.5-110 5.1 8

PtNPs/GO 25-5000 1.2 This work

Table S2 The recovery of standard addition of Cys in capsule

Sample Spiked (nM) Found (nM) Recovery (%) RSD (%)

0 773.8±13.95 1.8

500 1315±11.66 107.69±3.34 0.9

1000 1786±30.97 100.96±3.71 1.71

2500 3278±13.72 100.10±1.30 0.6

0 785.8±7.43 1.0

500 1312±18.93 106.02±4.73 1.5

1000 1710±7.95 92.85±0.63 0.52

2500 3230±32.91 96.60±2.45 1.5

0 799.3±8.13 1.0

500 1313±27.40 100.74±5.69 2.1

1000 1737±31.42 92.84±2.48 1.83

2500 3191±28.75 92.10±2.42 1.3

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