picture hamilton venus software intelligent liquid sensing

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Hamilton Venus Software Intelligent Liquid Sensing

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Intelligent Liquid Sensing

1. Capacitive Liquid Level Detection (cLLD)

2. Pressure Liquid Level Detection (pLLD)

3. Anti Droplet Control (ADC)

4. Capacitance Clot Detection

5. Monitored Air Displacement (MAD)

6. Total Aspiration and Dispense Monitoring (TADM)

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Conditions: Black tips or needles Conductive liquids only Clean or used tips All channels 96 head - A1 and H12 only 384 Head – A5 and P24 only Aspirate and or dispense steps

Capacitance Liquid Level Detection

More Details

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Pressure Liquid Level Detection

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Conditions: Black / clear tips Any liquid Clean tips, aspirate only All channels

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When to use cLLD & pLLD

Bubbles on liquid surface Use pLLD to find the liquid

surface cLLD could be triggered by the

bubbles

Aqueous layer under organic layer Use pLLD to find the non-

conductive organic layer Use cLLD to find the conductive

aqueous layer

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With ADC – the pressure increase inside the channel is measured and the piston moved to compensate.

Anti-Droplet Control

Without ADC – high vapour pressure of volatile liquid results in a pressure increase in the tip forcing the liquid out.

Plunger position

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Prevent droplets on tips when pipetting highly volatile liquids

This is a standard feature in VENUS

Anti-Droplet Control

Conditions: Black / clear tips or needles Channels only Pre-wetting tips improves

performance

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The conductive signal after aspiration is measured. gap between tip and liquid - the capacitance

signal will vanish. clot - it bridges the gap and the signal remains,

resulting in an error message

Capacitance Clot Detection

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Conditions: Black tips or needles Conductive liquids only Clean or used tips All channels

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Monitored Air Displacement

Tip Blocked

Pressure

Time

Correct Aspiration

Pressure

Threshold

Empty tip

Tip filling with liquid under vacuum

Tip filled with correct volume of liquid

Tip in liquid start aspirating

Tip blocked

Plunger stops moving, tip continues to fill

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Monitored Air Displacement

Conditions: Black / clear tips Clean tips, aspirate only All channels Lower limit varies but >10µl MAD has only been optimised for

aqueous liquids

MAD can be switched on for individual aspiration steps in a method

The pressure threshold can be varied to detect whether each pipetting step was successful

More Details

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Total Aspiration and Dispense Monitoring

TADM increases the safety and robustness of pipetting processes by recording and monitoring pressure throughout the pipetting cycle. It’s main use in in clinical settings where a permanent record showing correct pipetting of every sample is needed.

Conditions: Black / clear tips Clean tips All channels 96 TADM head Requires additional

TADM software

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Various pipetting errors change the pressure curves in interpretable ways

Aspirate

Dispense

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Aspirate Dispense


The user sets both TADM tolerance bands and the required response if they are crossed

Bands are set , using test data, for every liquid, tip type and volume used

picture hamilton venus software intelligent liquid sensing

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