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SERUM MRP 8/14 LEVELS IN PATIENTS WITH JUVENILE IDIOPATHIC ARTHRITIS Juvenile idiopathic arthritis (JIA) is the most common chronic inflammatory joint disease in childhood with prevalence one in 1000 children. Assessment of disease activity in children with JIA includes measurement of laboratory parameters of inflammation such as the erythrocyte sedimentation rate (ESR) or C- reactive protein (CRP). Recently, the levels of the pro-inflammatory S100 proteins, S100A8/9 (also known as calprotectin or myeloid-related protein (MRP) 8/14), in serum have been shown to correlate well with active joint count and with response to treatment. BACKGROUND: Samples were collected from 32 patients (23 girls and 9 boys) fulfilling the ILAR criteria for JIA (19 oligoarthritis, 13 polyarthritis) with a median age of 7,98 yrs. The groups were further subdivided according to time of diagnosis and course of disease. MRP 8/14 was measured by Bühlmann MRP8/14 Calprotectin ELISA kit (Bühlmann Laboratories, Switzerland). CRP and ESR were measured as part of routine clinical assessment. The levels of IL-6 were measured using immunoturbidimetric assay. Parametric tests were used for analyses. METHODS: CONCLUSION: Ganeva M., Stefanov St., Telcharova A., Mihaylova D. Department of Rheumatology, University Children's Hospital, Sofia, Bulgaria P87 The highest serum levels of MRP 8/14 were observed in the polyarthritis subgroup (n=6) with poor response to treatment – 14.71±8.80 μg/ml, followed by the polyarthritis subgroup (n=7) with newly diagnosed disease – 13.68±7.98 μg/ml. MRP8/14 concentrations were significantly higher in the polyarthritis subgroup (n=6) with poor response to treatment compared to the oligoarthritis subgroup (n=12) with disease flare (p<0,05). In the whole group good correlation was found between levels of MRP8/14 and CRP (r=0.72). Correlations between MRP8/14 and ESR (r=0.49), and MRP8/14 and IL-6 (r=0.50) were slightly lower. The levels of MRP 8/14 correlate well with the number of actively inflamed joints in part of the patients only. Highest levels of MRP8/14 were detected in the polyarthritis group which corresponds with the poorer prognosis of this group. A good correlation between concentrations of MRP8/14 and CRP was observed. A longitudinal follow-up of MRP8/14 serum levels is needed in order to monitor the disease activity, identify patients with higher risk for relapse, and response to treatment in individual patients. Foell D. et al. Arthritis Rheum. 2004;50(12):3762-71. Foell D. et al. JAMA. 2010; 303(13):1266-73. Gerss J. et al. Ann Rheum Dis. 2012;71(12):1991-7. Moncrieffe H. et al. Rheumatology (Oxford). 2013; 52(8):1467-76. Duurland C. et al. Curr Rheumatol Rep. 2014; 16(3):406. JIA Patients’ Subgroups Oligoarthritis Polyarthritis Newly diagnosed Poor response/ Disease flare n=7 n=12 n=7 n=6 RESULTS: High serum level of MRP8/14 was observed in 23 (65.7%) of the 32 children with JIA. The levels of MRP8/14 were significantly higher in the polyarthritis group compared to the oligoarthritis (p<0,05). Similarly, the levels of CRP and IL-6 were significantly higher in polyarthritis (p<0,05). Oligoarthritis Polyarthritis 0 2 4 6 8 10 12 14 16 MRP 8/14 Levels (mg/ml) Newly diagnosed Poor response/ Disease flare 0 5 10 15 20 25 30 35 40 45 ESR (mm/h) 0 10 20 30 40 50 60 CRP (mg/l) 0 10 20 30 40 50 60 70 IL-6 (pg/ml) Oligoarthritis Polyarthritis Oligoarthritis Polyarthritis Oligoarthritis Polyarthritis

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SERUM MRP 8/14 LEVELS IN PATIENTS WITH JUVENILE IDIOPATHIC ARTHRITIS

Juvenile idiopathic arthritis (JIA) is the most common chronic inflammatory joint disease in childhood with prevalence one in 1000 children. Assessment of disease activity in children with JIA includes measurement of laboratory parameters of inflammation such as the erythrocyte sedimentation rate (ESR) or C-reactive protein (CRP). Recently, the levels of the pro-inflammatory S100 proteins, S100A8/9 (also known as calprotectin or myeloid-related protein (MRP) 8/14), in serum have been shown to correlate well with active joint count and with response to treatment.

BACKGROUND:

Samples were collected from 32 patients (23 girls and 9 boys) fulfilling the ILAR criteria for JIA (19 oligoarthritis, 13 polyarthritis) with a median age of 7,98 yrs. The groups were further subdivided according to time of diagnosis and course of disease. MRP 8/14 was measured by Bhlmann MRP8/14 Calprotectin ELISA kit (Bhlmann Laboratories, Switzerland). CRP and ESR were measured as part of routine clinical assessment. The levels of IL-6 were measured using immunoturbidimetric assay. Parametric tests were used for analyses.

METHODS:

CONCLUSION:

Ganeva M., Stefanov St., Telcharova A., Mihaylova D.

Department of Rheumatology, University Children's Hospital, Sofia, Bulgaria

P87

The highest serum levels of MRP 8/14 were observed in the polyarthritis subgroup (n=6) with poor response to treatment 14.718.80 g/ml, followed by the polyarthritis subgroup (n=7) with newly diagnosed disease 13.687.98 g/ml. MRP8/14 concentrations were significantly higher in the polyarthritis subgroup (n=6) with poor response to treatment compared to the oligoarthritis subgroup (n=12) with disease flare (p