R011Potassium chromate, 5% w/v

IngredientsPotassium chromate 5.0 gmDistilled water 100.0 mlFinal pH ( at 25°C) 9.8±0.12

**Formula adjusted, standardized to suit performance parameters

DirectionsPlace 10 drops of urine in a test tube and add 1 drop of 5% potassium chromate (K2CrO4) solution as indicator. Add

dropwise 2.9% silver nitrate until a permanent and distinct colour change of red brown occurs.

Principle And InterpretationPotassium chromate indicator method is a precipitation titration method that uses potassium chromate (K₂CrO₄) as indicator

and silver nitrate (AgNO₃) as the standard solution. Add a small amount of K₂C rO₄ as indicator before starting determination,and then titrate with AgNO3 standard solution. After the start of the titration, the precipitate of white (silver chloride) or pale

yellow (silver bromide) precipitates first. When Cl-or Br-precipitates quantitatively, a little excess silver nitrate solution will

cause the concentration of Ag+ suddenly increasing to immediately generate brick red silver chromate (Ag₂C rO₄)

precipitation, indicating the titration endpoint. The chloride in the urine reacts with silver nitrate to precipitate as silver

chloride. Any excess of silver nitrate reacts with potassium chromate to form a reddish precipitate of silver chromate, the

appearance of which indicates the endpoint.

Composition**

Intended usePotassium Chromate, 5% w/v recommended for cholride estimation in urine sample.

Type of specimen Clinical samples: Urine

Specimen Collection and Handling: For clinical samples follow appropriate techniques for handling specimens as per established guidelines (1,2). After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions :In Vitro diagnostic Use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidleines should be followed while handling clincal specimens. Saftey guidelines may be referred in individual safety data sheets

Performance and EvaluationPerformace of the product is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Please refer disclaimer Overleaf.

Quality ControlAppearanceLemon yellow coloured solution.

ClarityClear with no insoluble particles.

pH9.72-9.92

Concentration4.90%- 5.10%

Disclaimer :

User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

TestPlace 10 drops of urine in a test tube and add 1 drop of 5% Potassium chromate (K2CrO4) solution as indicator. Add dropwise 2.9% silver nitrate with the same dropper until a permanent and distinct colour change to a red brown occurs.

ResultsThe number of drops of silver nitrate required to produce the colour change (red) expresses thesodium chloride content of theurine in grams per litre.

Reference1) Newell J.E.and Duke e., 1961,Workshop on urine analysis and renal function studies, the routine examination of urine inlaboratory, Chicago, American society of Clinical pathologist. 2) McFadden J.,1980,Biochemical Tests for identification of Medical Bacteria, 2nd ed., CRC Publication ,Clevland , Ohio

Revision : 02 / 2019

HiMedia Laboratories Technical Data

Storage and Shelf LifeStore between 10-30°C in tightly closed container and away from bright light. Use before expiry date on label. On opening, product should be properly stored in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use.

DisposalUser must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Followestablished laboratory procedures in disposing of infectious materials and material that comes into contact with clinicalsample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).

In vitro diagnostic

medical device

CE

Marking

Do not use if package is damaged HiMedia Laboratories Pvt.

Limited, B /4-6 , MIDC,

Dindori, Nashik MH

www.himedialabs.com

CE Partner 4U ,Esdoornlaan

13, 3951 DB Maarn The

Netherlands,

www.cepartner 4u.eu

IVD

Storage temperature10°

C

30°C

EC REP

HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: techhelp@himedialabs.com Website: www.himedialabs.com