Nutrition and chronic diseases S67

PP118-SUNASSOCIATION BETWEEN APOLIPOPROTEIN A-II (APOA2)POLYMORPHISM AND INSULIN RESISTANCE IN OBESEADOLESCENTSM.E. Zaki1, K. Amr2, M. Abdel-Hamid2. 1BiologicalAnthropology, 2Medical Molecular Genetics, NationalResearch Centre, Cairo, Egypt

Rationale: The APOA2 gene has been associated with obesityand insulin resistance (IR) in previous studies with controver-sial results. This study aims to investigate association betweena functional APOA2 promoter polymorphism ( 265T>C)) and IRand in a sample of Egyptian obese adolescents.Methods: Cross-sectional study was undertaken in 350adolescents (207 females and 143 males), aged 17 19years. They were 180 obese (BMI >P97) and 170 withnormal weight, who participated in the project entitled“ Obesity among Youth: Lifestyle and Genetic Factors”funded by the Science and Technology Development Fund.Anthropometric, clinical and biochemical variables weredetermined. Homeostasis model assessment of IR (HOMA-IR) was used. The study analyzed interactions between theAPOA2 265T>C polymorphism and anthropometric measuresand IR. Genotyping of 265T>C polymorphism was carried outusing polymerase chain reaction-restriction fragment lengthpolymorphism (PCR-RFLP) analysis.Results: Prevalence of CC subjects ranged from 13% to17%. Males and females were analyzed together becausehomogeneity of the effect was observed by sex. Homozygousindividuals for the 265C allele had statistically higher BMI,waist to hip circumference, body fat percentage, visceraladipose tissue and HOMA-R level than in carriers of the Tallele. Homozygous individuals for the 265C allele had higherobesity risk than carriers of the T allele. The mutant C allele ofthe APOA-II gene was more frequent in obese cases comparedwith normal weight cases in both sexes.Conclusion: The most consistent effects of the 265T>Cpolymorphism were observed on anthropometric variables andhomozygous CC individuals had higher IR risk than carriers ofthe T allele in obese subjects.

Disclosure of Interest: None Declared

PP119-SUNTHE EVALUATION OF THE CLINICAL VALUE OF HOMEENTERAL NUTRITION (HEN)

S. Klek1, G. Dziwiszek2, K. Matysiak3, P. Szybinski4,K. Szczepanek1, A. Galas5. 1General and Oncology SurgeryUnit, Stanley Dudrick’s Memorial Hospital, Skawina,2Department for General and Vascular Surgery, MazowieckiSpecialist Hospital, Ostroleka, 3Chair of General,Oncology and Gastroenterology Surgery, Poznan MedicalUniversity, Poznan, 4General and Oncology Surgery Unit,Stanley Dudrick’s Memorial Hospital, Skawina, 5Chairof Epidemiology and Preventive Medicine, JagiellonianUniversity Medical College, Krakow, Poland

Rationale: Home enteral nutrition (HEN) is thought to bea life-saving medical procedure, which helps to recover,to prevent the malnutrition and to avoid its consequences.Unfortunately, the scarcity of data does not allow to preciselyassess its clinical value in terms of organ function, nutritional

parameters and clinical outcomes. The aim of the study wasto analyze clinical value of HEN.Methods: The group of 453 patients (219 F, 234 M, 312 adults,141 children, mean age 43.2), fed for at least 12 months bytube with both: 1/ self-made mixed kitchen diet (initially)without any medical supervision and then 2/ food for specialmedical purposed under the surveillance of HEN center. Toevaluate treatment efficiency of HEN anthropometric andlaboratory data as well as clinical outcomes (hospitalizationrate, length of hospital and ICU stay) were analyzed duringthe first and the second part of the treatment.Results: Weight gain was significantly increased in bothchildren and adults (+43.2% and 11.9%, respectively, p < 0.01).In children the concentration of hemoglobin increased by 4%(p = 0.006), and SGPT decreased by 3.2% (p = 0.001). The meanincidence of hospitalization fell from 1.98/patient/year to1.26/patient/year, p < 0.001), and the mean length of hospitalstay fell from 39.1 days to 11.9/ year (p < 0.001). Thosedifferences were observed in both, adults and children.Conclusion: HEN represents a very efficient treatment option,which allows to reduce the incidence of hospitalization andits length. It also allows to improve nutritional anthropometricand laboratory parameters.

Disclosure of Interest: S. Klek Speakers bureau of: Nutricia, B Braun,Fresenius, Baxter, Nestle, G. Dziwiszek: None Declared, K. Matysiak:None Declared, P. Szybinski: None Declared, K. Szczepanek: NoneDeclared, A. Galas: None Declared

PP120-SUNOVERWEIGHT IS ASSOCIATED WITH UNBALANCED OFADIPOCITOKINES IN ADOLESCENTSA.P.Q. Mello1, T.M. Sampaio1, N.R.T. Damasceno1.1Department of Nutrition, School of Public Health,University of Sa Paulo, Sa Paulo, Brazil

Rationale: Obese individuals are a very heterogeneouspopulation. Adipocytokines secreted by adipose tissue playmajor roles in the regulation of energy metabolism andcould modulate lipid metabolism. The aim of this study wasto investigate the adipocitokines profile in adolescents withnutritional status different.Methods: 156 adolescents, aged 10 19 years, both sexes fromSa Paulo (Brazil) were studied. Anthropometric parameterswere monitored. The subjects were classified as Obese-OB,Overweight-OW or Normalweight-NW. Glucose, insulin, lipidprofile and adipocitokines were evaluated. Statistical analysiswere performed by SPSS® (p < 0.05).Results: It was not observed differences in relation sex,puberty and physical exercise. NW and OB were older thanOW (p = 0.02). BMI (p < 0.001), WC (p < 0.001) and fat mass(p < 0.001) increased according nutritional status. Glucose,TG, total cholesterol and LDL-C were similar between groups.OB showed higher values for insulin (p = 0.005, p < 0.001),HOMA (p = 0.025, p < 0.001), systolic blood pressure (p = 0.007,p < 0.001) than OW and NW. HDL-C in OB was lowerthan NW (p = 0.049). Correlation between resistin and BMI(r = 0.37 p < 0.001), WC (r = 0.38 p < 0.001), HDL-C (r = 0.19p = 0.016) and SBP (r = 0.31 p = 0.003) were founded. In relationadiponectin, there were correlation with BMI (r = 0.45p < 0.001), WC (r = 0.46 p < 0.001), TG (r = 0.19 p = 0.016),HOMA (r = 0.26 p = 0.001), SBP (r = 0.22 p = 0.037) and HDL-C(r = 0.27 p = 0.001). For leptin, it found correlation with BMI

S68 Poster presentations

(r = 0.66 p < 0.001), WC (r = 0.63 p < 0.001) and HOMA (r = 0.56p < 0.001). Obesity in adolescents is related with resistinand leptin increased and adiponectin reduced (p < 0.001).Resistin (b = 0.14 p = 0.033), leptin (b = 0.52 p < 0.001) andadiponectin (b = 0.19 p = 0.005) were associated with BMIvariability (r2 = 0.41).Conclusion: Adipocitokines may be important pathogenicfactors in obesity and cardiovascular diseases in adolescents.Financial Support: Fapesp 07/57601 5, 07/57602 1.

Disclosure of Interest: None Declared

PP121-SUNMODULATION OF SERUM OXYLIPIN LEVELS BY FISH OILSUPPLEMENTATION IN NORMO- AND HYPERLIPIDEMIC MENJ.P. Schuchardt1, S. Schmidt1, B.D. Hammock2, A. Hahn1,N.H. Schebb3. 1Institute of Food Science and HumanNutrition, Leibniz University of Hannover, Hannover,Germany; 2Department of Entomology & Cancer ResearchCenter, University of California, Davis, CA, United States;3Institute of Food Toxicology and Analytical Chemistry,University of Veterinary Medicine, Hannover, Germany

Rationale: Many effects of polyunsaturated omega-3 fattyacids (n-3 PUFAs) such as eicosapentaenoic acid (EPA)and docosahexaenoic acid (DHA) on lipid metabolism andinflammatory pathways are thought to be mediated by theiroxidized metabolites (oxylipins). However, the effect of dietsrich in EPA and DHA on oxylipin levels is poorly investigated.Methods: In the present study, we compared oxylipin patternsof 20 hyperlipidemic (cholesterol >200 mg/dl; triglyceride>150 mg/ml) and 20 normolipidemic men after 12 wk of fish oilintake. Levels of 45 free hydroxy-, dihydroxy- and epoxy-FAswere analyzed in serum by LC-MS. Additionally, oxylipin levelswere compared with their parent PUFA levels in erythrocytemembranes; the most suitable marker for assessing PUFAstatus.Results: Oxylipin levels varied strongly among subjects. Noor minor differences in the oxylipin pattern were observedbetween normo- and hyperlipidemic subjects before andafter treatment. In all subjects, levels of most EPA-derivedoxylipins (170 4,800 pM) were considerably elevated afterfish oil intake (150 1,400%), the increase of DHA-derivedoxylipins (360 3,900 pM) is less pronounced (30 130%). Incontrast, the effect on arachidonic acid-derived oxylipinlevels (140 27,100 pM) was inconsistent. Whereas levels ofAA-derived epoxy- and dihydroxy-FA are significantly reduced(15 40%) after fish oil treatment, some of its hydroxy-FA werefound to be increased (up to 180%). The relative change ofEPA in erythrocyte membranes is strongly correlated (r� 0.5;p < 0.05) with the relative change of corresponding epoxy- anddihydroxy-FA serum levels.Conclusion: Our results demonstrate that nutrition has adecisive influence on oxylipin patterns, which suggests thatoxylipin levels can be directly influenced by the diet. Furtherstudies need to investigate causal reasons for interindividualdifferences.

Disclosure of Interest: None Declared

PP122-SUNWEIGHT LOSS IN PARKINSON’S DISEASE?S. Lindskov1,2,3, K. Sjoberg3, A. Westergren1, P. Hagell1.1School of Health and Society, Kristianstad University,2Department of Geriatrics and Neurology, Central HospitalKristianstad, Kristianstad, 3Department of Clinical Sciences,Lund University, Lund, Sweden

Rationale: A number of previous reports have observed thatunintentional weight loss is a common problem without knowncause in Parkinson’s disease (PD). The aim with this study wasto investigate nutritional status and its relation to diseaseduration in PD.Methods: Data from 71 home-dwelling people with PD,without significant cognitive impairment (mean age, 67.3years; 56% men; mean PD duration, 6.3 years) were collectedcross-sectionally regarding PD motor symptoms, disability,involuntary movements (dyskinesias), body weight (kg), BMI,nutritional risk (SCREEN II), handgrip strength (HGS) and mid-upper arm muscle circumference (MUAMC). The sample wasdivided into those with longer and shorter PD duration (asdefined by the median, 5.5 years).Results: Longer PD duration (n = 34) was associated withsignificantly more motor symptoms, disability and dyskinesiascompared to shorter PD duration (P� 0.04). Mean±SD bodyweight and BMI were 80.3±16.3 kg and 28.1±4.8, respectively,and did not differ between longer vs. shorter PD durationgroups (79.6±18.4 vs. 80.9±14.4 kg, and 28.3±5.3 vs. 28±4.2;P� 0.738). Similarly, there were no differences in SCREEN IIscores (46±6.5 vs. 45.8±6.9), HGS (39.3±16.8 vs. 36.2±14.3),or MUAMC (24.9±4.1 vs. 24.1±4) between longer vs. shorterPD duration groups (P� 0.400).Conclusion: In contrast to previous studies we did not findevidence of underweight in PD, neither among those withshort nor long disease duration, while other disease markersdiffered as expected according to PD duration. The reason(s)for our unexpected findings are not yet clarified. Longitudinalstudies are warranted in order to verify our observation andidentify possible explanations.

Disclosure of Interest: None Declared

PP123-SUNPILOT STUDY OF INTESTINAL MICROBIOTA DIVERSITYIN SEVERE MALNOURISHED PATIENTS WITH ANOREXIANERVOSA (AN)

M. Hanachi Guidoum1,2, F. Levenez3, E. Latour1,N. Cournede1, J. Dore3, P. De Truchis1, J.C. Melchior1,2,P. Crenn1,2. 1Nutrition Unit, Hopital Raymond Poincare(APHP), 2EA4497, UVSQ, Garches, 3INRA, Institut MICALIS,Jouy en Josas, France

Rationale: Functional digestive disorders and metabolicdisturbances are described during undernutrition in AN.Associations between changes in the microbiota, inflammatoryand metabolic diseases have been demonstrated in various sit-uations. Nevertheless microbiota in undernutrition is scarcelyinvestigated.Methods: We studied intestinal microbiota diversity profileof malnourished AN patients. The aim was to identifypossible changes in the microbiota and look for associationswith digestive and metabolic disturbances during AN. Eight