Researchers Jariya Jiriyasin Jittikan Narapan

Sinnapa Vanduangden

AdvisorAdvisor Mr. Sorachai Saelim Mr. Sorachai Saelim

IntroductionIntroduction

• Treatment of acne and other skin eruptions such as sores, (Aburjai T, Phytotherapy Research 2003)

• Important for normalizing oily/acne skin and balances dry skin (Myra Michelle Eby, 2006)

(http://www.comav.upv.es/taxonomy_intro.html)

(http://your-cosmetics.com) (http://cyborg9.exteen.com/20070518/entry)

• To extract the oil from Cucurbita moschata Decne seeds

• To produce liquid soap from Cucurbita moschata Decne seed oil

• To test the quality of liquid soap

ObjectiveObjective

ExperimentsExperiments

1. Extraction of the oil from Cucurbita moschata Decne seeds by Soxhlet extractor

3. Test the quality of liquid soap2. Production of liquid soap

Measure the foam capacity

Measure the pH value

Test the ability of emulsifying paraffin oil

Studying the reaction of liquid soap with Ca2+, Mg2+ and Fe3+

Studying the reaction of liquid soap with inorganic acid

Measuring the satisfaction of using the liquid soap

Experiments

1. Extraction of the oil by Soxhlet extractor1. Extraction of the oil by Soxhlet extractor

ResultsResults

Table 1. Show the character and quantity of Cucurbita moschata Decne seed oil

Weight of the seeds (g)

Quantity of the oil (ml)

Physical characteristic of the oil

picture

770 235.8 The oil is liquid and quite sticky. It has green-brown colors

2. Production of liquid soap2. Production of liquid soap

Oils

Dissolve KOH in water

The oils were mixed from the formulas

Table 2. Show the ratio of Cucurbita moschata Decne seed oil to other oils that used to produce liquid soap (% by mass)

formulaOils

1 2 3 4 5 6 7 8 9

Coconut oil 40 30 30 50 50 - 100 - -

Palm oil 30 40 30 50 - 50 - 100 -

Pumpkin seed oil

30 30 40 - 50 50 - - 100

2. Production of liquid soap2. Production of liquid soap

(1) (2) (3)

(6)

(5)(4)

(40:30:30) (50:50:0) (50:0:50)

(0:50:50)

(30:30:40)(30:40:30)

(7)

(100:0:0)

(8)

(0:100:0)

(9)

(0:0:100)

PS (Coconut oil : Palm oil : Pumpkin seed oil)

Produced Liquid soapsProduced Liquid soaps

Measure the foam capacity

Measure the pH value

Test the ability of emulsifying paraffin oil

Studying the reaction of liquid soap with Ca2+, Mg2+ and Fe3+

Studying the reaction of liquid soap with inorganic acid

Measure the satisfactions by using liquid soaps

3. Test the quality of liquid soap3. Test the quality of liquid soap

3. Test the quality of liquid soapTest the quality of liquid soap

Weighed liquid soap from each formula 5 g

Dissolve in 50 ml - DO water

Test the quality of liquid soap Liquid soap solutions

Preparation of the solution of liquid soapPreparation of the solution of liquid soap

Add each solution of liquid soap into test tube, 4 ml per tube

Shake each test tube

Observe and write down the results

Measure the foam capacityMeasure the foam capacity

1.852

2.25 2.252.4

2.25

2.652.55

2.9

4

3.35

2.8

0

0.5

1

1.5

2

2.5

3

3.5

4

1 2 3 4 5 6 7 8 9 10 11 12

123456789101112

Results of measuring foam capacityResults of measuring foam capacity

Chart 1. Show the results of measuring foam capacity

Measure the pH value of each solution of liquid soap by pH - meter

The pH value of each solution was written down

Adjust pH meter by buffer solution

Measure the pH valueMeasure the pH value

Results of measuring the pH valueResults of measuring the pH value

pH

formulas

Chart 2. Show the results of measuring the pH value

10.1 10.12 10.11 10.07

10.36

10.63

9.73

10.7710.53

10.12 10.11

9.6

8

8.5

9

9.5

10

10.5

1 2 3 4 5 6 7 8 9 10 11 12

123456789101112

Add paraffin oil into two test tubes, 10 drops per tube

Add the solution of the 1st formula of liquid soap into test tubes, 5 ml per tube then shake

The results of testing the ability of emulsifying paraffin oil were observed and written down

Do this experiment to each solution of liquid soap

Test the ability of emulsifying paraffin oilTest the ability of emulsifying paraffin oil

Results of emulsifying paraffin oil testResults of emulsifying paraffin oil test

Formula The results

DO water The solution has no color, there is the oil layer on the surface 5 mm high. It separates obviously

Formula 1 The solution has clear yellow color, there is the oil layer between the foam and the solution 2 mm high

Formula 2 The solution has light yellow color, it is turbid, there is the oil layer between the foam and the solution 3 mm high

Formula 3 The solution has clear yellow color, there is the oil layer between the foam and the solution 1.75 mm high

Table 3. Show the results of emulsifying paraffin oil test

Formula The results

Formula 4 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 2.5 mm high

Formula 5 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 1.5 mm high

Formula 6 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 2 mm high

Formula 7 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 2 mm high

Formula 8 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 2 mm high

Results of emulsifying paraffin oil testResults of emulsifying paraffin oil test

Formula The results

Formula 9 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 1.75 mm high

Formula 10 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 2.5 mm high

Formula 11 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 1.5 mm high

Formula 12 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 1.5 mm high

Results of emulsifying paraffin oil testResults of emulsifying paraffin oil test

The

results

were

written

down

Liquid soap solution

CaCl2

MgCl2

FeCl3

Paraffin oil

Studying the reaction of liquid soapStudying the reaction of liquid soap with Cawith Ca2+2+, , MgMg2+2+ and Fe and Fe3+3+

The

results

were

written

down

ResultsResults

Formula Reagents

1 % CaCl2 1 % MgCl2 1 % FeCl3

1 there are white dregs. there is no dreg in the tube. there is a big piece of brown-red dregs.

2 there are turbid white dregs. there are turbid white dregs.

there is a big piece of brick-red dregs.

3 there are turbid white dregs. there are turbid white dregs.

there is a big piece of brick-red dregs.

4 there are turbid white dregs. there are turbid white dregs.

there are turbid orange dregs.

5 there are soft yellow dregs. there are soft yellow dregs. there are turbid orange dregs.

6 there are turbid white dregs. there are soft yellow dregs. there are turbid orange dregs.

Table 4. Show the results of studying the reaction of liquid soap with Ca2+, Mg2+ and Fe3+

Formula Reagents

1 % CaCl2 1 % MgCl2 1 % FeCl3

7 there are white dregs. there are white dregs. there are orange dregs.

8 there are turbid white dregs. there are turbid white dregs.

there are turbid orange dregs.

9 there are soft yellow dregs. there are soft yellow dregs. there is no dregs.

10 there is no dregs. there is no dregs. there are turbid orange dregs.

11 there are turbid white dregs. there are turbid white dregs.

there are turbid orange dregs.

12 there is no dregs. there are turbid white dregs.

there are turbid orange dregs.

ResultsResults

ResultsResults

Table 5. Show the results of studying the reaction of liquid soap with Ca2+, Mg2+ and Fe3+ then with paraffin oil

Formula Reagents

1 % CaCl2 1 % MgCl2 1 % FeCl3

1 there is oil layer. there is oil layer. there is a round piece of fat and dregs.

2 there is oil layer. there is a round piece of fat and dregs.

there is a round piece of fat and dregs.

3 there is oil layer. there is a round piece of fat and dregs.

there is oil layer.

4 there is no oil layer. there is oil layer. there is a round piece of fat and dregs.

5 there is oil layer. there is oil layer. there is a round piece of fat and dregs.

Formula Reagents

1 % CaCl2 1 % MgCl2 1 % FeCl3

6 there is a round piece of fat and dregs.

there is a round piece of fat and dregs.

there is oil layer.

7 there is oil layer. there is no oil layer. there is a round piece of fat and dregs.

8 there is oil layer. there is oil layer. there is a round piece of fat and dregs.

9 there is oil layer. there is oil layer. there is oil layer.

10 there is oil layer. there is oil layer. there is oil layer.

11 there is oil layer. there is oil layer. there is oil layer.

12 there is oil layer. there is oil layer. there is oil layer.

ResultsResults

Studying the reaction of liquid soapStudying the reaction of liquid soap with inorganic acidwith inorganic acid

ResultsResults

Table 6. Show the results of studying the reaction of liquid soap with inorganic acid

Formula Process

Add HCl Add KOH Burning

1 there are dregs. dissolved in KOH there is no flame.

2 there are dregs. dissolved in KOH there is flame when burned.

3 there are dregs. dissolved in KOH there is no flame.

4 there is no dregs. there is no dregs. there is no flame.

5 there is no dregs. there is no dregs. there is no flame.

6 there are dregs. quite dissolved in KOH there is no flame.

Formula Process

Add HCl Add KOH Burning

7 there are dregs. quite dissolved in KOH there is no flame.

8 there are dregs. dissolved in KOH there is flame when burned.

9 there are dregs. dissolved in KOH there is no flame.

10 there are dregs. dissolved in KOH there is no flame.

11 there are dregs. dissolved in KOH there is no flame.

12 there are dregs. dissolved in KOH there is no flame.

ResultsResults

Measuring the satisfactions of using the liquid soapsMeasuring the satisfactions of using the liquid soaps

Measuring the satisfactions of using the liquid soaps by 90 MWIT students with questionnaires, then the results were

analyzed by SPSS 11.5

Section 1, Inquire the samples about personal information.

Section 2, Inquire the samples about the satisfactions when using the liquid soaps such as produced foam, smell, colors and cleaning.

Questionnaires

ResultsResults

Chart 3. Show the ages of samples Chart 4. Show the personal ailment of samples

Color Smell Foam Advertisement Cleaning Price Others Total

Frequency(%)

11(5.76)

69(36.13)

8(4.19)

33(17.28)

43(22.51)

20(10.47)

7(3.66)

191(100)

ResultsResults

Table 7. Show the behavior of buying soap of samples

the level of satisfactions points

good quality and great satisfactionsgood quality and good satisfactionsgood quality and fairly good satisfactionsbad quality and not satisfy

- 350.400.

250. - 3.4 9

150. - 2.4 9

1.00 - 1.49

Table 8. Show the standard of satisfied levels of liquid soaps

Measuring the satisfaction of using the liquid soapsMeasuring the satisfaction of using the liquid soaps

Chart 5. Show the averaged satisfactions of using the liquid soaps

ResultsResults

ConclusionConclusion

- From the extraction of oil in pumpkin seeds oil, the oil content is about 30.6 %

- From the experiments, the formula 10 has the most foam capacity in compare with all the liquid soap and the formula 9 has the most foam capacity in compare with 9 formulas produced liquid soap.

- The formula 5, 11, 12 has the most ability to emulsify paraffin oil. As the paraffin oil is lipid with composed in dirt.

ConclusionConclusion

- From the experiment of measuring pH value, the formula 7 has the least pH value in compare with 9 formulas produced liquid soap, which is 9.73.

- From the measuring of satisfactions value of using liquid soap, the formula 7 , 8 and 9 satisfied the samples respectively.

AcknowledgementAcknowledgement

• Mr. Sorachai Saelim

• Department of Chemistry, Mahidol Wittayanusorn school

ReferencesReferences

African Journal of Biotechnology Vol. 4 (1), pp. 36-44, January 2005

Aburjai T, Natsheh FM. Plants used in cosmetics. Phytotherapy Research 2003;17:987–1000

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