present project

Researchers Jariya Jiriyasin

Jittikan NarapanSinnapa

VanduangdenAdvisorAdvisor Mr. Sorachai Mr. Sorachai SaelimSaelim

IntroductionIntroduction• Treatment of acne and other skin eruptions such as sores, (Aburjai T, Phytotherapy Research 2003 )• Important for normalizing oily/acne skin and balances dry skin (Myra Michelle Eby, 2006 )

(http://www.comav.upv.es

/taxonomy_intro.html)

(http://your-cosmetics.com) (http://cyborg9.exteen.com/20070518/entry)

• To extract the oil from Cucurbita moschata Decne seeds

• To produce liquid soap from Cucurbita moschata Decne seed oil

• To test the quality of liquid soap

ObjectiveObjective

ExperimentsExperiments

1. Extraction of the oil from Cucurbita moschata Decne seeds by Soxhlet extractor

3. Test the quality of liquid soap2. Production of liquid soap

Measure the foam capacity

Measure the pH value

Test the ability of emulsifying paraffin oil

Studying the reaction of liquid soap with Ca2+, Mg2+ and Fe3+

Studying the reaction of liquid soap with inorganic acid

Measuring the satisfaction of using the liquid soap

Experiments

1. Extraction of the oil by 1. Extraction of the oil by Soxhlet extractorSoxhlet extractor

ResultsResults

Table 1. Show the character and quantity of Cucurbita moschata

Decne seed oilWeight of the seeds

(g)

Quantity of the oil (ml)

Physical characteristic

of the oil

picture

770 235.8 The oil is liquid and quite sticky. It has green-brown colors

2. Production of liquid 2. Production of liquid soapsoap

Oils

Dissolve KOH in water

The oils were mixed from the formulas

Table 2. Show the ratio of Cucurbita moschata Decne seed oil to

other oils that used to produce liquid soap (% by mass)

formulaOils

1 2 3 4 5 6 7 8 9

Coconut oil

40

30

30

50

50

-100

- -

Palm oil30

40

30

50

- 50 -100

-

Pumpkin seed oil

30

30

40

-50

50 - -100

2. Production of liquid 2. Production of liquid soapsoap

(1) (2) (3)

(6)

(5)(4)(40:30:

30)(50:50:0)

(50:0:50)

(0:50:50)

(30:30:40)

(30:40:30)

(7)(100:0:0)

(8)(0:100:0)

(9)(0:0:100)

PS (Coconut oil : Palm oil : Pumpkin seed oil)

Produced Liquid Produced Liquid soapssoaps

Measure the foam capacity

Measure the pH value

Test the ability of emulsifying paraffin oil

Studying the reaction of liquid soap with Ca2+, Mg2+ and Fe3+

Studying the reaction of liquid soap with inorganic acid

Measure the satisfactions by using liquid soaps

3. Test the quality of 3. Test the quality of liquid soapliquid soap3. Test the quality of liquid Test the quality of liquid soapsoap

Weighed liquid soap from each formula 5 g

Dissolve in 50 ml - DO water

Test the quality of liquid soap Liquid soap solutions

Preparation of the solution Preparation of the solution of liquid soapof liquid soap

Add each solution of liquid soap into test tube, 4 ml per tube

Shake each test tube

Observe and write down the results

Measure the foam Measure the foam capacitycapacity

1.852

2.25 2.252.4

2.25

2.652.55

2.9

4

3.35

2.8

0

0.5

1

1.5

2

2.5

3

3.5

4

1 2 3 4 5 6 7 8 9 10 11 12

123456789101112

Results of measuring Results of measuring foam capacityfoam capacityChart 1. Show the results of measuring

foam capacity

Measure the pH value of each solution of liquid soap by pH - meter

The pH value of each solution was written down

Adjust pH meter by buffer solution

Measure the pH valueMeasure the pH value

Results of measuring the Results of measuring the pH valuepH value

pH

formulas

Chart 2. Show the results of measuring the pH value

10.1 10.12 10.11 10.07

10.36

10.63

9.73

10.7710.53

10.12 10.11

9.6

8

8.5

9

9.5

10

10.5

1 2 3 4 5 6 7 8 9 10 11 12

123456789101112

Add paraffin oil into two test tubes, 10 drops per tube

Add the solution of the 1st formula of liquid soap into test tubes, 5 ml per tube then shake

The results of testing the ability of emulsifying paraffin oil were observed and written down

Do this experiment to each solution of liquid soap

Test the ability of Test the ability of emulsifying paraffin oilemulsifying paraffin oil

Results of emulsifying paraffin Results of emulsifying paraffin oil testoil test

Formula

The results

DO water

The solution has no color, there is the oil layer on the surface 5 mm high. It separates obviously

Formula 1

The solution has clear yellow color, there is the oil layer between the foam and the solution 2 mm high

Formula 2

The solution has light yellow color, it is turbid, there is the oil layer between the foam and the solution 3 mm high

Formula 3

The solution has clear yellow color, there is the oil layer between the foam and the solution 1.75 mm high

Table 3. Show the results of emulsifying paraffin oil test

Formula

The results

Formula 4

The solution has white color, it is turbid, there is the oil layer between the foam and the solution 2.5 mm high

Formula 5


Formula 6

The solution has white color, it is turbid, there is the oil layer between the foam and the solution 2 mm high

Formula 7


Formula 8



Formula

The results

Formula 9


Formula 10


Formula 11


Formula 12



The

results

were

written

down

Liquid soap solution

CaCl2

MgCl2

FeCl3

Paraffin oil

Studying the reaction of liquid Studying the reaction of liquid soapsoap with Cawith Ca2+2+, Mg, Mg2+2+ and Fe and Fe3+3+

The

results

were

written

down

ResultResultss

Formula

Reagents

1% CaCl2 1% MgCl2 1% FeCl3

1 there are white dregs.

there is no dreg in the tube.

there is a big piece of brown-red dregs.

2 there are turbid white dregs.

there are turbid white dregs.

there is a big piece of brick-red dregs.



there is a big piece of brick-red dregs.



there are turbid orange dregs.

5 there are soft yellow dregs.

there are soft yellow dregs.





Table 4. Show the results of studying the reaction of liquid soap

with Ca2+, Mg2+ and Fe3+

Formula

Reagents


7 there are white dregs.

there are white dregs.

there are orange dregs.




9 there are soft yellow dregs.


there is no dregs.

10 there is no dregs. there is no dregs. there are turbid orange dregs.




12 there is no dregs. there are turbid white dregs.


ResultResultss

ResultResultssTable 5. Show the results of studying the

reaction of liquid soap with Ca2+, Mg2+ and Fe3+ then with

paraffin oilFormula

Reagents


1 there is oil layer. there is oil layer. there is a round piece of fat and dregs.

2 there is oil layer. there is a round piece of fat and dregs.

there is a round piece of fat and dregs.

3 there is oil layer. there is a round piece of fat and dregs.

there is oil layer.

4 there is no oil layer.

there is oil layer. there is a round piece of fat and dregs.


Formula

Reagents


6 there is a round piece of fat and dregs.


there is oil layer.

7 there is oil layer. there is no oil layer.



9 there is oil layer. there is oil layer. there is oil layer.




ResultResultss

Studying the reaction of liquid soapStudying the reaction of liquid soap with inorganic acidwith inorganic acid

ResultResultssTable 6. Show the results of studying the

reaction of liquid soap with inorganic acid

Formula

Process

Add HCl Add KOH Burning

1 there are dregs. dissolved in KOH there is no flame.

2 there are dregs. dissolved in KOH there is flame when burned.


4 there is no dregs. there is no dregs. there is no flame.

5 there is no dregs. there is no dregs. there is no flame.

6 there are dregs. quite dissolved in KOH

there is no flame.

Formula

Process

Add HCl Add KOH Burning

7 there are dregs. quite dissolved in KOH

there is no flame.

8 there are dregs. dissolved in KOH there is flame when burned.





ResultResultss

Measuring the satisfactions of using Measuring the satisfactions of using the liquid soapsthe liquid soaps

Measuring the satisfactions of using the liquid soaps by 90 MWIT students with questionnaires, then the results were

analyzed by SPSS 11.5

Section 1, Inquire the samples about personal information.

Section 2, Inquire the samples about the satisfactions when using the liquid soaps such as produced foam, smell, colors and cleaning.

Questionnaires

ResultResultss

Chart 3. Show the ages of samples

Chart 4. Show the personal ailment of samples

Color

Smell

Foam

Advertisement

Cleaning

Price

Others

Total

Frequency(%)

11(5.76)

69(36.13)

8(4.19)

33(17.28)

43(22.5

1)

20(10.47)

7(3.66)

191(100

)

ResultResultss

Table 7. Show the behavior of buying soap of samples

the level of satisfactions

points

good quality and great satisfactionsgood quality and good satisfactionsgood quality and fairly good satisfactionsbad quality and not satisfy

- 350 4.00

250. - 3.4 9

150. - 2.4 9

1.00 - 1.49

Table 8. Show the standard of satisfied levels of liquid soaps

Measuring the satisfaction of using the Measuring the satisfaction of using the liquid soapsliquid soaps

Chart 5. Show the averaged satisfactions of using the liquid soaps

ResultResultss

ConclusionConclusion

- From the extraction of oil in pumpkin seeds oil, the oil content is about 30.6 %

- From the experiments, the formula 10 has the most foam capacity in compare with all the liquid soap and the formula 9 has the most foam capacity in compare with 9 formulas produced liquid soap.

- The formula 5, 11, 12 has the most ability to emulsify paraffin oil. As the paraffin oil is lipid with composed in dirt.

ConclusionConclusion

- From the experiment of measuring pH value, the formula 7 has the least pH value in compare with 9 formulas produced liquid soap, which is 9.73.

- From the measuring of satisfactions value of using liquid soap, the formula 7 , 8 and 9 satisfied the samples respectively.

AcknowledgementAcknowledgement

• Mr. Sorachai Saelim

• Department of Chemistry, Mahidol Wittayanusorn school

ReferencesReferences

African Journal of Biotechnology Vol. 4 (1), pp. 36-44, January 2005

Aburjai T, Natsheh FM. Plants used in cosmetics. Phytotherapy Research 2003;17:987–1000

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