prezentácia programu powerpoint · smart-lamp quantitatively detect diverse pathogens in blood,...
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Smartphone based methods
Smartphone
Miniature computers
Price
Routine test
Diagnostic
Point of care
Smartphone
As detector
As instrumentalinterface
Anemia detection
Smartphone
As detector
As instrumentalinterface
Breath-analyzer
Breath-analyzer
ECG
Semenanalysis
Loop mediatedisothermalamplification(LAMP)
LAMP vs PCR
LAMP
• Isothermal
• 4 primers
• Bst polymerase
PCR
• Series of temperature changes
• 2 primers
• Taq polymerase
https://www.youtube.com/watch?v=L5zi2P4lggw
https://www.youtube.com/watch?v=V4PyySdk3Jo
LAMP advantages and disadvantages
• High amplification yield
• Tolerance to inhibitors
• Short time
• Detection systems
• False positive results
• Primer dimer
• Large fragment
Pathogen detection
! TIME !
smaRT-LAMP
• Real-Time LAMP
• Rapid, quantitative detection system
• Time frame and lower cost
Bacticount app
smaRT-LAMP
Quantitatively detect diverse pathogens in blood, urine and feces
Shorter time-frame
(1h vs. 18-28h)
Diagnostic tool for Urinary tractinfections (UTIs)
Smart cup
Smart cup platform
Simple
Inexpensive
Minimally instrumented
Does not require any lab facilities
Polymorphism genotyping
Single nucleotide polymorphism (SNP)
3´AS-LAMP 5´AS-LAMP
Colorimetric detection
• 3´ AS-LAMP – low dyeconcentration
• 5´ AS-LAMP – high dyeconcentration
ASO-LAMP
Application:
Point of care
Detection of pathogens
Genotyping
enVisionenzyme-assisted nanocomplexes
for visual identification of nucleic acids
Enzyme-DNA nanostructures
• Recognition
• Universal signaling
➢Decoupled targetrecognition
➢Signal amplification
Step 1: sample loading Step 2: recognition
Step 3: target-independent signal enhacement Step 4: visual detection
enVision LAMP
Advantages
• Sensing mechanism:
➢Signal enhancement
➢Direct hybridization
➢Universal visual enhancement
• Assay programmability:
➢Decoupling recognition / signaling
➢More probe choices
➢Multiplexing
Application:
Rapid viral mutations
Infectious diseases
High-prevalence infections
Point of care
One computer on every desk
and one in every home
Bill Gates
