Proceedings

of the 13th European Microscopy Congress

Antwerp, Belgium, August 22-27,2004

Edited byDominique Schryvers and Jean-Pierre Tirnmermans

Volume III

Life Sciences

Edited byJean-Pierre Verbelen and Eddie Wisse

Belgian Society for Microscopy

Liege

I

Contents

Plenary Lectures

PLl HRTEM vs HRSTEM: are these instruments complementary or competitive ? 3

HaiderMax. MuUer H., HartelP.

PL2 Energy resolution: how low canwe go 1 5

Colliex Christian. GloterA., Imhoff D., Stephan 0., Tence M.

PL3 Exploring the inner space of cells by cryoelectron-tomography 7

Baumeister Wolfgang. Beck Martin, Briegel Ariane, CyrklaffMarek, Keil Thomas, Kuemer

Julia, Lucie Vladan, Medalia Ohad, Nickell Stephan, Plitzko Juergen

Special Interest Lectures

SILl Microscopy in art and archaeology 11

Brunetti Brunetto Giovanni

SIL2 Microscopy with photons 13

Parker Ian

SIL3 Microscopy in semiconductor industry 15

VeirmanAnn E.M.. Otterloo A.F.

SEL4 Structure andperformance of bioadhesiondevices: learning from flies and geckos 17

Arzt Eduard. GaoHuajian, Gorb Stanislav, Spolenak Ralph

SH5 Microscopic and metallurgical aspects of the Space Shuttle Columbia accident investigation andreconstruction 19

McDanels Steve

LS 1. Nucleus: structure and dynamics (Ueli Aebi)

LS1.I1 Structural dissection of nucleoporin function JH.23

Schwarz - Herion K., Paulillo S.M., Koser J., Maco B., Aebi U., Fahrenkrog Birthe

LS1.I2 Nuclear Pathfinders: On the organization ofinterphase chromatin and the dynamics ofthe

interchromatin compartment 111.25

Herrmann Harald. Richter Karsten, Reichenzeller Michaela, Dreger Christine, Lichter Peter

LS1.01 Molecular motor proteins in DNA transcription HI.27

Philimonenko V.V., Dingova H., Kysela K., Kahle M., Iben S., Grummt I., de LanerolleP.,Hozak Pavel

LS1.02 Mechanisms of nuclear envelope assembly in growingXenopus oocytes using endoplasmicreticulum vesicles m.29

Morozova Ksenia. Kiseleva Elena

LS1.03 Three-dimensional image reconstruction of native monomelic spliceosomes by single particle

cryo-electron microscopy IH.31

Azubel M., WolfS., Sperling J., Sperling R.

LS1.P01 Influence of the murine cytomegalovirus M97-protein on DNA encapsidation andon nuclear

membranes: a study based on high- pressure freezing JH.33

Buser Christopher. Mertens Thomas, Walther P., Michel Detlef

LS1.P02 Interchromosomal congests of nuclear bodies JH.35

Richter Karsten. Reichenzeller Michaela, GSrisch Sabine M., Schmidt Ute, Dreger Christine,

Nessling Michelle, Herrmann Harald, Lichter Peter

LS1.P03 Ultrastructural study of the nucleolus during oogenesis in the oviparous teleost fish, Barbus barbus (L.) IH.37

ThirvMarc. PoncinP.

LS1.P04 What is first: neuronal degenerationor nuclear inclusions of Ataxin 3? Neuronal nuclear inclusions

in SCA3 human brain: Ataxin3 - polyglutarnin colooalisation and cellular distribution

in pontine brain centres. HI.39

DiikFreark. Blaauw E.H., Brunt E.R.P., de Vos R.A.I., van der Want J.J.L.

IX

LS 2. Immuno - in situ - reporter gene constructs (Jean-Pierre Verbelen)

LS2.I1 Use of fluorescent proteins to dissect the dynamics of theplant secretory pathway M.43

Brandizzi Federica. Hawes Chris, Hanton Sally L, Renna Luciana, Stefano Giovanni

LS2.I2 FLIP as a tool to study the molecular organisation of nuclear lamins m.45

Broers Jos. Dupuis G., Kuijpers H.J.H., Endert J., Worman H., Ostlund C, Ramaekers F.C.S.

LS2.01 SNA-binding sites in the humangastric glands. A lectin ultrastructural localization of sialic acid HI.47

Madrid Juan F.. G6mez Laura, Saez Francisco J, Godi nez JoseM, Garcia-Garcia Maria J,Hernandez Francisco

LS2.02 Immunocytochemical localization ofCramoll in Cratylia mollis seeds JJI.49

dos Santos Ana Celia Oliveira. Peixoto C.A, CoelhoL.C.B.B.

LS2.03 On the role of elastin-heparan sulphate interactions HI.51

Gheduzzi Dealba, Pepe Antonietta, Buchicchio Barbara, Pasquali-Ronchetti Ivonne,

Tamburro Antonio, Ouaglino Pamela

LS2.04 Testicular nitric oxidelevels and apoptosis in rat testis during inguinal hernia repair after usinglong term mesh bioprosthesis IJJ.53

Vatansever Seda. Taneli Fatma, Kaymaz Figen, Kose Can, Muftuoglu Sevda, Hkgul Ozer

LS2.05 Application of molecular localizationtechniques to the study of thebiological function of allergensin olive (Olea europaea L.) pollen UJ.55

Along Juan de Dios. M'rani-Alaoui Mohammed, Harnman-Khalifa AbdelMounim, Castro

Antonio Jesus, Olmedilla Adela, Jimenez-L6pez Jose Carlos, Suarez Cinthya,

Rodriguez-Garcia Maria Isabel

LS2.06 Multichannel imaging ofcomplexreceptor-like glutamatergic nerve terminals in whole mount

preparations of the visceral pleura using a dual spinning disk confocal microscope UI.57

Pintelon IsabeL Brouns Inge, De Proost Ian, Van Meir Frans, Timmermans Jean-Pierre,Adriaensen Dirk

LS2.P01 Relationship between the Langerhans cells of epidermis and dentritic cells of lymphoreticular system:an immunohistochemical study HI.59

Elmas CiBdem. Erdogan D.

LS2.P02 Hypersensitive response in plants : microscopic evidence that HR is a form ofprogrammed cell death m.61

Auriac Marie-Christine. Lorrain S., Roby D., Balague C.

LS2.P03 Cryopreparation for immunolocalization of a schistosoma mansoni membrane receptor HJ.63

Ben Younes Amena. Vicogne J., Spehher D., Capron M.., Dissous C.

LS2.P04 Contribution ofiNOS andoxidative stress to coronary endothelial dysfunction postinfarction IU.65

Berges An. VanNassauw Luc, Timmermans Jean-Pierre, Vrints Chris

LS2.P05 Immunoelectronmicroscopical detection of prion protein (PrP) on PrP-displayingretrovirus-like particles HI.67

Boiler Klaus. Nikles Daphne, Dreher Wilfried, Merten Christoph, Montrasio Fabio, Cichutek

Klaus, Buchholz Christian J.

LS2.P06 Cell death in the early chicken embryo: in situ detection of apoptotic cells and developmentalexpression ofACINUS UI.69

DewulfEls. Goeman Els, Harrisson Fernand

LS2.P07 Spinning disk confocal study of the distribution of P/Q-type Ca2+channels related to pulmonaryneuroepithelial bodies m.71

De Proost Ian. Brouns Inge, Pintelon Isabel, Timmermans Jean-Pierre, Adriaensen Dirk

LS2.P08 Lactoferrin expression in human endometrial carcinoma cells - light and electron microscopicimmunogold studies 111.73

Staneva-Dobrovsld Ludmilla. Niederacher D., Denkova R.

LS2.P09 Vasoactive intestinal polypeptide-immunoreactive elements in the olfactory bulb of the

musk shrew, Suncus murinus IE. 75

Kakuta Sachiko. Takayanagi Masaaki, Kimura Akihiko, Oda Satoko, YangJun Li, Chen

Shao Yun, Gomi Toshiaki, Kisbi Kiyoshi

LS2.P 10 Cellulose fibril orientation confers anisotropy in cell wall extensibility to the epidermis of onion bulbs UI.77

Souslov Dmitry, Foubert Sonja, Kuypers Stefan, Kemps Raymond, Verbelen Jean-Pierre

X

LS2.P11 Identification of GalNAc-glycoconjugates in pro-acrosomal vesicles during spermiogenesisin the urodele Pleurodeles waltl 111.79

Madrid Inan F Fernandez Elena, Saez Francisco J, Hervella Montserrat, G6mez Laura,Valbuena Galder, Hernandez Francisco

LS2.P12 Unequal distribution of GalNAc-glycoconjugates in the acrosome of the spermatozoon of the urodelePleurodeles waltl Ul.il

Madrid Juan F.. Hervella Montserrat, Saez Francisco J, Fernandez Elena, G6mez Laura,Valbuena Galder, Hernandez Francisco

LS 3. Membrane trafficking (Catherine Rabouille)

LS3.I1 COPI dependent sorting of glycosylation enzyme in the Golgi apparatusNilsson Tommy

LS3.I2 Caveolin-stabilized membrane domains as multifunctional transport and sorting devices in

endocytic membrane traffic m.85Pelkmans Lucas

LS3.01 COPI controls Golgi morphology by segregatingtransmembrane area asymmetry into vesicles HJ.87Beznoussenko G.V., Burger K.N.J., Fusella A., Koster A.J., Geerts W., Luini A., Mironov

Alexander A.

LS3.02 Immunogold ultrastructural localization ofmenkes protein in rat parotid acinar cell III.89D'Amico Fabio

LS3.03 Membranetraffic andthepolar distribution ofGurken and Oskar protein and mRNA in

Drosophila oocyte JH.91Rabouille Catherine. Herpers B., Oprins A.

LS3.04 Dynamics of Golgi membranes in Herpesvirus envelopment HI.93Wild Peter. Senn C, Engels M., Biennkowska - Szewczyk K., Schraner E.M., Walther P.,

MiillerM.

LS3.05 Fission complex assembly during synpatic vesicle recycling JH.95Capani Francisco. Sundborger Anna, Evergren Emma, Masich Sergej, CrumJohn, Tomilin

Nikolay, Low Peter, Ellisman Mark, Brodin Lennart, Shupliakov O.

LS3.06 Eosinophil secretion: novel insights into granule structure and cytokine trafficking in activated cells UI.97Melo Rossana. Spencer Lisa, Perez Sandra, Dvorak Ann, Weller Peter

LS3.P01 Effect of the plant alkaloid voacamine on the P-glycoprotein function in cultured multidrug resistantosteosarcoma cells UJ.99

Meschini Stefania, Marra Manuela, Calcabrini Annarcia, Condello Maria, Federici Elena,Galeffi Corrado, Dupuis Maria Luisa, Cianfriglia Maurizio, Arancia Giuseppe

LS3.P02 Shape and size of in situ cryo-fixed bovine herpesvirus type 1 UXlOlSchraner Elisabeth Maria. Engels M., LoepfeE., Bienkowska-SzewczykK., Walther P.,

Muller M., Wild P.

LS3.P03 Enzyme composition ofCOPI vesicles isolated from rat liver Golgi IJJ.103Micaroni Massimo. Beznoussenko G.V., FusellaA., Luini A., MironovA.A.

LS3.P04 The intracellular pathways of cuticular chitin and (glyco)protein synthesis in the integument of

moulting crabs, Carcinus maenas, as followedbythe use ofgold-conjugated lectin probes HI. 105

Compere Philippe. Bouchtia H., Goffinet G.

LS3.P05 Combination of confocal microscopy and digital image restoration to visualize the uptake ofultrafine particles in lung cells m.107

Rothen-Rntishanser Barbara. Schurch Samuel, Geiser Marianne, Gehr Peter

LS3.P06 Ultrastructural features of the vitellaria in the land planarian Geoplana burmeisteri (Platyhelrninthes,Tricladida) HI. 109

Falleni Alessandra. Lucchesi Paolo, Ghezzani Claudio, Silveira Marina, Gremigni Vittorio

LS3.P07 Electron tomography of transcytosis in endothelial cells of human arteries HI. 111

SpekL.Y.. Humbel B.M., Geerts W.J.C., Lahpor J.R., Koster A. J., Verkleij A. J., Post J.A.

XI

LS 4. The cytoskeleton (Benjamin Geiger)

LS4.I1 Structural studies of actin-branches mediated bytheArp2/3 complex HI. 115

Rouiller Isabelle, Egile Coumaran, Kaiser Donald, Nicastro D., Kim Eldar, PollardThomas,Li Rong, VolkmannNiels, Hanein Dorit

LS4.D Actin remodeling in filopodai and phagocytic caps revealedby cryo - electron tomography ELI17

Medalia Ohad. Beck M., Gerisch G., Baumeister W.

LS4.01 Investigation of the microtubular cytoskeleton of Ustiiago maydiswing high pressure freeze

fixation and cryo substitution HL119

Hause Gerd. StraubeAnne, Steinberg GeroLS4.02 In vivo measurements reveal a model for the alignment of microtubules in root hairs of

Arabidopsis thaliana HI.121

Van BruaeneNathalie. Joss Greg, Van OostveldtPatrick

LS4.P01 Plant cytoskeleton in vivo and in vitro III. 123

Matienco Boris. Maximova E., Brezeanu A.

LS4.P02 Diazepam induces aberrant chromosome segregation and apoptosis-like death in cultured tumor cells III. 125

Crateri Pasqualina. VitaleHio, Antoccia Antonio, Leone Stefano, Arancia Giuseppe,Tanzarella Caterina

LS4.P03 C2C12 myoblasts as a model to study skeletal muscle differentiation DJ.127

Battistelli Michela, Burattini S., Ferri P., Curci R., Luchetti F., Falcieri E.

LS4.P04 In situ identification of nestin- and vimentin-positive bipolar cells in acute multiple sclerosis lesions HI. 129

Vandenabeele Frank. Moreels Marjan, Struys Tom, Lambrichts Ivo, Ameloot Marcel,

Stinissen Piet

LS 5. Protein dynamics/interactins/FRAP-FRET (Rainer Pepperkok)

LS5.I1 Imaging cytoskeleton and signalling dynamics in live cells with GFP-based 4D-microscopy,FRET and FLIM III. 133

Gadella Theodoras, van MunsterErik, Dhonukshe Pankaj, Adjobo-Hermans MereL Vermeer

Joop, Kremers Gert-Jan, Goedhart Joachim

LS5.I2 Observing the spatio-temporal dynamics of proteins in the secretory pathway with light microscopy EI. 135

Weiss Matthias

LS5.01 FRET analysis reveals a direct protein-protein interactionbetweenmembers ofboth FtsZ families in

Physcomitrellapatens UI.137

Hause Bettina. Keissling Justine, Reski Ralf, Decker Eva L.

LS5.02 Rapid constriction of lipid bilayers by the mechanochemical enzyme dynamin IH.139

Danino Dganit. Moon Kwan-Hoon, Hinshaw Jenny E.

LS 6. Biomaterials (Robert GeoffRichards)

LS6.I1 Microscopic techniques for cultured cells in tissue engineering research HI. 143

Kirkpatrick Charles James. Peters K., Hermanns M.I., Krump-Konvalinkova V., Fuchs S.,

Unger R.E.

LS6.I2 SEM ofbiomaterials and biomaterial tissue interlaces HT.145

Richards Geoff

LS6.01 TEM and SEM studies ofcomplex microcapsules from biodegradable polymers for drug encapsulation III. 147

Blanchin Marie-Genevieve. Teodorescu Valentin, Serban, Al Haushey Lamaz, Briancon

Stephanie, Fessi Hatem

LS6.02 Rough surfaces of titanium and titanium alloys for implants and prostheses HI. 149

Conforto Eele. Aronsson B.-O., Salito A., Crestou C, Caillard D.

LS6.03 On the real-structure of biomimetically grown fluorapatite-gelatine-composites ITL151

Simon Paul Carillo-Cabrera S., Formanek P., Gobel C, Geiger D., Ramlau R., Kniep R.

LS6.04 Bioactivity process on glass particles at the submicrometer scale studied by EDXS/STEM HI. 153

Banchet Vincent Jallot E., Michel J., Balossier G.

LS6.P01 Biological validation of calcified sponges as recorders of climate change: followinguptake of

trace elements using X-ray microanalysis HI. 155

Berry Lorraine. Willenz Philippe, Calmars Group

XII

LS6.P02 Analytical TEM characterisation of nitrided multilayered titanium-base biomaterials HI.157

Czvrska-Filemonowicz Aleksandra. BuffatPhilippe - A., Moskalewicz Tomasz, Lucki

Mariusz, Biel Magdalena, RakowskiWieslaw

LS6.P03 Biodegradaticm of platinum implants afterlong term insertion into the rat subcutane tissue IEL159

Jonas Ludwig. Fulda G., Labs W., Viek U.

LS6.P04 Transmission electron microscopy of carboxylated cellulose nanocristals UI.161

Mohamad Roumani, Montanari S., Paintrand Isabella Vignon M.R.

LS6.P05 Electron microscopic examinationoftissue engineeredheart valve protheses after 3 months

implantation in an ovine model 10.163

Schmohl Kathleen. Grabow Niels, Stamm Christof, SteinhoffGustav, Behrend Detlef,Schmitz Klaus-Peter

LS6.P06 Effect of donor age onthe quality of the extracellular matrix in the skin equivalent model IU.165

Sammarco Rita, Croce Maria Antonietta, Gheduzzi Dealba, Tiozzo Roberta, Sommer Pascal,Damour Odile, Pasquati-Ronchetti Ivonne, Ouaglino Daniela

LS6.P07 TEM, SuperSTEM, and EELS studies of the structure, chemistry and biological function of

ferritin inhuman liver biopsies IU.167

PanY.. Brown A., BrydsonR., Warley A., Li A., Powell J., Bleloch A., Falke U, Falke M.,Calvert C.C.

LS 8. SEM for Life Sciences (Tolo ap Gwyn)

LS8.11 Low-voltage SEM images from biological specimensarj Gwvnn Iolo

IU.171

LS8.12 Low temperature scanning electron microscopy and x-ray microanalysisEchlin Patrick

in.173

LS8.01 The sternal CaC03 deposits of Porcellio scaber (Crustacea): an ultrastructural study of a

biomineralisation process

Fabritius Helae. Zieptler Andreasrn.i75

LS8..02 Morphological features of sensitive and Micafungin-resistant Candida albicans

Strinearo Annarita. Aneiolella Letizia. Colone Marisa. De Bernardis Flavia, Toccacieli

Laura, Ciocci Alessandra, Palamara Anna Teresa, Arancia Giuseppe, CassoneAntonio

in.177

LS8,.P01 Improvements for HR- and Cryo-SEM bythe VCT 100 high-vacuum cryo transfer system and

SEM cooling stage

WepfRoeer. Richter T.. SattlerM.. Kaech A.

in. 179

LS8,.P02 Novel methods for scanning electron microscopy to visualize the adhesion site ofthe cultured

eukaryotic cells

Lounatmaa Kari

nxm

LS8..P03 Flower and fruit structure of lansium domesticum corr.

Yunus Abdul Ghani. Razak AbdulRahman. Wahab Zakiria

IE. 183

LS8..P04 Microscopy and chemical study of the seeds of Opuntia ficus-indica prickly pear fruit

Habibi Youssef. Dupevre Danielle. Mahrouz Mustapha. Visnon Michel

in.185

LS8.P05 Importance ofSEM in the studying of microscopic algae (dinophyta)Krakhmalnw Alexander. Krakhmalnw Maxim

in.187

LS8..P06 Ultrastructure of zona pellucida before and after fertilization

Akkovunlu Gokhan. Er Hakan. Ustunel Ismail. Demir Ramazan

in.189

LS8..P07 SEM observations of rnicrovascularization and resolution with osseous healing in peri-implant tissues IH.191

Kishi Yoshiaki

LS8..P08 Fibroblastic reticulum cells ofhuman spleenPolak S.. Kopani Martin. Michalka P.. Durdik S.. Jakubovskv J.. Zitnanova I.

in. 193

LS8..P09 Dense particles in pons cerebrum ofa patient with the neurological form of Behcet disease

Physical-chemical properties ofdense particlesKopani Martin. Rychty B.. KriStin J.. Jorik V.. Mikula M.. Jakubovskv J.. Kalina P..

Wlachovska B., Zimanska I., Polak S.

m.i95

LS8,,P10 SEM observation of tooth enamel and dentin after adhesive preparation.Roubalikova Lenka. Autrata Rudolf. Wandrol Petr

in.i97

XIII

LS 9. Biological applications ofscanning probe microscopy (Andreas Engel)

LS9.I1 Organization of rhodopsin and opsin in native disk membranes unveiled by electron and atomic force

microscopy m.201Fotiadis Dimitrios. Suda K., Liang Y., Filipek S., Palczewski K., Engel A.

LS9.I2 Atomicforce microscopy of the bacterial photosynthetic apparatus IH.203Scheuring Simon. Rigaud J.-L.

LS9.01 AFM andTEM studies ofsome oligomeric/polymeric intermediates during the aggregationprocessof the Ap,_40 amyloid peptide m.205

Lai Kee Him Josephine. Talaga P., Quere L., Brisson A.

LS9.02 AFM of biological material embedded in epoxy resin UI.207

Matsko Nadezda. Mueller Martin

LS9.03 Direct detection ofa gene on DNA molecule in nano-meter scale using scanning near field

optical / atomic force microscope UL209Ohtani Toshio. Kim Jong Min, Yoshino Tomoyuki, Nakao Hidenobu, Sasou Megumi,

Sugiyama Shigeru, Hirose Tamaki, Muramatsu Hiroshi

LS9.P01 Investigation of barley chromosome structure by scanning near-field optical/atomic force microscopy UI.211Yoshino Tomovuki. Fukushi Daisuke, Shichiri Motoharu, Matsuto Takayuki, Sugiyama

Shigeru, Hagiwara Shoji, Akazawa Kohei, Ushiki Tatsuo, Ohtani Toshio

LS 10. Cryo-electron microscopy/tomography ofcells and organelles (Marin Van Heel)

LS10.I1 Three-dimensional representation of bone ingrowth in calcium phosphate biomaterials usingSynchrotron X-ray microtomography UI.215

Weiss Pierre. Rau Christophe, Pilet Paul, Obadia Laeticia, Magne David, Bourges Xavier,Weitkamp Turn, Khairoun Ibrahim, Gauthier Olivier, Daculsi Guy

LS10.I2 Humidity control- the key to successful preparation of cells and suspensions for 3D cryo

electron microscopy III.217Frederik Peter

LS10.01 Cryo-TEM of dispersed mesosphases IJJ..219

Saealowicz Laurent. Rouvet Marline, Leser Martin, Watzke Heribert, Michel Martin, de

Campo Liliana, Yaghmur Anan, Glatter Otto

LS10.O2 Direct imaging cryo-TEM of gold nanoparticle growth within multilamellar vesicles 1H.221

Regev Oren. Faure Chrystel, Roux Didier

LS10.O3 Automated cryo tomography at liquid nitrogen and liquid helium temperatures HC.223

Busing Wim. de Haas Felix, Lucken Uwe, Melanson Linda, Hax Werner

LS10.O4 3D structure of melibiosepermease, a sugar cotransporter from Escherichia coli 111.225

LundbackAnna-Karin. Purhonen P., Leblanc G., Hebert Hans

LS10.O5 Three-dimensional structure of Herpes simplex virus 1 from electron cryotomography m.227

Griinewald Kav. Desai Prashant, Winkler Dennis C, Heymann J. Bernard, Belnap David M.,Baumeister Wolfgang, Steven Alasdair C.

LS10.O6 Ultrastructural study of excitatory synapses with cryo-electron tomography m.229

Yang Ting. Lucie Vladan, Baumeister WolfgangLS10.P01 Mapping the Thermoplasma proteome - structural proteomics studies using free-flow electrophoresis

and cryo-electrontomography ET.231

Kofler Christine. Nickell S., BoicuM., Baumeister Wolfgang

LS10.P02 Effect ofbeam tilt in the electron microscope on alignment of gold-markers for electron tomography IH.233

Masich Sergei

LS10.P03 Domain motions during gating of a K. channel inside the membrane UL235

Vemen-Brvan Catherine. Kuo Anling, Johnson Louise, Doyle Declan

LS 11. EM crystallography and quantitative electron diffraction (Hans Hebert)

LSI 1.11 Cyttron: a window on the molecular machinery of life HI.239

Abrahams Jan Pieter. Van Dyck Dirk, Frampton C, Hax Werner, van Heel Marin,

Hoedemaeker P.J., Tanke H., Verkleij Arie, Young I.

LS11.12 Expression, two-dimensional crystallization and structure analysis of water channel in brain JJX.241

Hiroaki Yoko. Tani Kazutoshi, Kamegawa Akiko, GyobuNobuhiko, Walz Thomas,Kuwahara Michio, Sasaki Sei, Mitsuoka Kaoru, Fujiyoshi Y.

XIV

LSI 1.01 Advances in automated orientation mapping in the TEM BX243

Dingley David

LS11.02 Structure ofOprM, aPseudomonas aeruginosa efflux pump, determined by electron crystallography HI.245

Jouan I.udovic. Lambert 0., Benadbelhak H„ Ducruix A., BrissonAlain

LSI 1.03 Solving the phase problem for electron diffraction on non-centrosymmetric membraneproteinstructures IH.247

Koch Christoph

LSI1.04 Microsomal glutathionetransferase 1 - structurefromtwo different crystal forms JH.249

Holm Peter. Bhakat Priyaranjan, Jegerscheid Caroline, Gyobu Nobuhiko, Mitsuoka Kaoru,

Fujiyoshi Yoshinori, Morgenstern Ralf, HebertHans

LS11.05 The structure of biogenic silica compared to silica gel Ht.251

Holzhiiter Gerd. Narayanan Kamatchi, Gerber Thomas

LSI 1.06 Nano- and microstructure of normal andosteoporotic human vertebra bones H1.253

Suvorova Elena. Petrenko Pavel, Titov Anatoly, BuffetPhilippe

LS11.P01 Enhanced nucleation of protein crystals in external electric fields due to electrically-driven convection IBL255

Penkova Anita. Dimitrov Ivaylo, Hodjaoglu Feyzim, Vekilov Peter

LS11 .P02 Renal Na,K-ATPase structure from cryo-electron microscopy with 2-D crystals showing structural

variability HI.257

Purhnnen Pasi. Grundberg Bjorn, AlvangRonny, KoeckPhilip, ThomsenKaren, Hebert

Hans, Maunsbach ArvidB.

LSI 1 .P03 Study of silica structure in Spelt (Triticum spelta) by electron microscopy m.259

Narayanan Kamatchi, Holzhuter Gerd. Gerber Thomas

LS11.P04 Alpha-amylolysis ofmodel A-type starch crystals HI.261

Pohu Anne, Putaux Jean-Luc. Buleon Alain, Planchot Veronique, Colonna Paul

LS 13. Life cell imaging (Rosario Rizzuto)

LS13.I1 Real-time imaging ofcAMP fluctuations monitored in cardiac myocytes by GFP-taggedPKA

and FRET ffl.265

Zaccolo Manuela

LS13.I2 Confocal, two-photon and dual two-photon microscopy in studies of polarized epithelial cells UI.267

Chvanov M., Ashby M., Gerasimenko J., Gerasunenko O., DolmanN., Barrow S.,

Sherwood M., Voronina S., Petersen O.H., Tepikin Alexei

LS13.01 Optical multisite recording of [Ca2+]i with confocal microscopy as a tool complementary to

conventional electrophysiology in the study of neuro-immune interactions 1H.269

DeJonge Frederik. Kroese A.B.A., De Laet A., Van NassauwL., Miller H.R.P., Van

Bogaert P.-P., Timmermans Jean-Pierre

LS13.02 Clustering ofhemodynamicresponse functions from fMRI-data of a starling BX271

Smolders Alain. Scheunders Paul, Sijbers Jan, Van Meir Vincent, VerhoyeMarleen, Van der

Linden Annemie

LS13.03 Disturbed somitogenesis leads to vertebral segmentation defects JH.273

Tmni Kenji, Kokubu Chikara, Fujimoto Shoji, KokubuTomoko, Balling Rudi, Heinzmann

Ulrich

LS13.04 Laser microtools for studies on a heart tissue model 10.275

Perner Birgit. Monajembashi S., Greulich K.O.

LS13.P01 Automatedimage analysis system for clone detection in the colony formationassay m.277

Van deWouwer Gert. Verhaeghe Steven, Bakker Anette, Van Strien Miriam, Van Dun

Jacky, Janicot Michel, VanDyckDirk, Weyn Barbara

LS13.P02 Fast multicolour high resolution imaging of subcellular details and dynamics in living transfected

cells using a microlens-enhanceddual spinning disk confocal setup. Comparison with classical

single beam confocal microscopyIII.279

Brouns Inge. Pintelon Isabel, DeProost Ian, RaesAdam, Snyders Dirk, Timmermans Jean-

Pierre, Adriaensen Dirk

LS13.P03 Microscopy as essential tool to unravel the mechanism of controlled cell elongation in

Arabidopsis thaliana DI.281

De Cnodder Tinne, Vissenberg Kris. Foubert Sonja, Verbelen Jean-Pierre

LS13.P04 4D-imaging of living yeast cells: analysing protein and organelle dynamics in vivo UI.283

Wolinski Heimo. Kohlwein Sepp D.

XV

LS13.P05 Selective visualization of neuroepithelial bodies in living rat lung - slices by styryl pyridinium dyes ni.285Pintelon Isabel. De Proost Ian, Van Genechten J., Brouns Inge, VanMeir Frans,

Tirnmermans Jean-Pierre, Adriaensen Dirk

LS13.P06 The occurrence and possible role of epiphytic organisms on the pneumatophores of the mangrove JH.287Naidoo Y,. Steinke T.D., Naidoo G.

LS 14. Single particle reconstruction ofmacromolccular aggregates (Jose L. Carrascosa)

LS14.I1 V-ATPases in comparison to F-and A-ATPases HI.291

Bottcher Bettina. Domgail Ines, Mellwig Christian, Fischer Susanne, Diepholz Meikel,Venzke David, Fethiere James

LS 14.12 3D structure determination of snRNPs and spliceosomesby single particle electron cryomicroscopy DI.293

Sander B., Golas M, Boehringer D., Fischer N., WillC, Kastner B., Luehrmann R., Stark

Holger

LS14.01 Structure of the HSV-1 portal protein by electron microscopy IJX295

Trus Benes. Newcomb Wittiam, Cheng Naiqian, Brown Jay, Steven Alasdair

LS14.02 A single mutation in GroEL converts the allosteric mechanism fromconcertedto sequential IJX297

Rivenzon-Sepal Dalia. Danziger Oded, GrayerWolfS., Horovitz Amnon

LS14.03 Visualization of viral RNA by cryo-electron microscopy JU.299

Koning Roman, van den Worm S.H.E., Plaisier J.R., WannenhovenH.J., Abrahams J.P., vanDuin J., Koerten H.K.

LS14.04 Single particle electron microscopy in combination with proteomics to investigate novel complexesofmembrane proteins IJ1301

Arteni Ana-Andreea. Lax J, Koufil R, R6gner M, Boekema E.J.

LS14.05 Structure of a twisted protease: Cryo-electron microscopy of DrosophilaTripeptidyl Peptidase H JU.303

Rockel Beate. Peters Jurgen, Glaeser Robert, BaumeisterWolfgang

LS14.06 Stainless models of rapana thomasiana hemocyanin 1H.305

Chenp Kimberlev. Koeck P.J.B., Idakieva Krassimira, Ternstrom Tomas, Parvanova Katja,Hebert Hans

LS14.P01 Structural changes in photosystem I and photosystemII during state transition in thealga

Chlamydomonas reinhardtii KI.307

Yakushevska Alevtyna. Ihalainen Janne, KeegstaWilko, Oostergetel Gert, Rochaix Jean-

David, Dekker Jan, BoekemaEgbertLS14.P02 Structural polymorphism in the archaeal helicase MCM fromMethanobacterium thermoautotrophicumEi.309

San Martin Carmen, G6mez Yacob, Fletcher Ryan J., Chen Xiaojiang S., Carazo Jose-Maria

LS14.P03 Position ofV-ATPase stalk subunits deduced from a F,-/V,-ATPasehybrid complex HI.311

Chaban Yuriy. Coskun tj., Grfiber Gerhard, Keegstra Wilko, Oostergetel Gert, Boekema

Egbert

LS14.P04 Subunit domain localization in theinfluenzavirus polymerase complex JH.313

Martin-Benito Romero Jaime. Area Estela, Gastaminza Pablo, Torreira Eva, Valpuesta Jose

Maria, Ortin Juan, Carrascosa Jose L.

LS14.P05 The interaction of phosducin-like protein (PhLP) with the cytosolic chaperonin CCT: a structural

and functional study HI.315

Martin-Benito Romero Jaime. Bertrand Sara, Hu Ting, Ludtke Paul, McLaughlinJoseph N.,Willardson Barry M., Carrascosa Jose L., Valpuesta J.M.

LS14.P06 Electron microscopical investigation of the effect of y-ionizing radiation on the association of

protozoa, bacteria and viruses, models of polyxenic cultures of entamoeba IH.317

Hovnanvan Karlen. HovnanyanM.K., Karageuzyan K.G.

LS14.P07 Imaging ofcyanophycin in bacterial cells using energy filtering transmission electron microscopy IH.319

Koop Anne. Kohl Helmut, Thesing Andreas, Vop Ingo, Elbahloul Yasser, Steinbiichel

Alexander, Reichelt Rudolf

LS14.P08 Association of the chlorophyll-binding protein IsiA with photosystem I in the cyanobacterium

SynechocystisPCC6S03 HL321

Koufil Romaa Yeremenko Nataliya, Ihalainen Janne A., D'Haene Sandrine, HagemannMartin, Matthijs Hans CP., Dekker Jan P., Boekema Egbert J.

LS14.P09 Detection of an allergen-containing crystal in dry pollengrains aiPhleumpratense after

anhydrous fixation UI.323

GroteMonika. Krzyzanek Vladislav, Valenta Rudolf, Swobodalnes, Reichelt Rudolf

XVI

LS14.P10 Structural characterisation of the type III secretion apparatus of Shigellaflexneri HI.325

SaniMusa. Oostergetel Gert, Keegstra Wilko, Boekema Egbert

LS14.P11 Preliminary model of the three-dimensional structure of insect lipoprotein HDLpby combiningelectron microscopy and X-ray crystallography JJI.327

Valentijn Karine, Koning Roman, Derks Yvonne, Van Doom Jan, Van der Krifl Theo,

Schouten Arie, Van der Horst Dick, Gros Piet, Koster Abraham, Rodenburg Kees

LS ISab. Diagnostic EM in infectious diseases and pathology (Hans R. Gelderblom, Werner

Jacobs)

LS15.I1 Electron microscopic diagnosis of infectious diseases and surveillance of emerging diseases

and bioterrorism agents HI.331

Miller Sara E.

LS15.I2 Rapid diagnostic electron microscopy in infectious diseases: an update UI.333

Gelderblom Hans R.

LS15b.I2 Development of a web-accessible SEM/EDX database ofgun shot residue particles using a neural

network search engine UI.335

Nvs Bart, Niewoehner Ludwig, Gunaratnam Lawrence

LS15.01 Electron microscopy ofSARS-coronavirus infection: characteristics of infection in broncho-alveolar

lavage cells and in Vero cell culture HL337

Kolesnikova Larissa. Brodt Hans-Reinhard, Klenk Hans-Dieter, Becker StephanLS15.02 Diagnosing Norovirus gastroenteritis outbreaks in Spain by electron microscopy: a four year

survey (2000-2003) IJX339

Herrera Maria-Inmaculada. Vivo Amparo, Perez-PastranaEsperanza, de la Loma Asia

LS15.03 Ultrastructural changes of the tracheal epithelium uponvaccination with the La Sota strain of

Newcastle disease virus III.341

Mast Jan, Nanbra Cecile, van den Berg Thierry, Meulemans GuyLS 15.04 Rapid diagnosis by electron microscopy of viral infections in urine from transplant patients: a five

years survey (1999-2003) HI.343

Cuevas Laureano. Fernandez del Campo Juan Alfonso, Santa Maria Ysmael, Varela Jose

Maria, de la Loma Asia, Herrera Maria Inmaculada

LS15.05 Microwaveprocessing speeds up EM diagnosis of pathological tissues and potential bioterrorism

samples HI.345

Schroeder Josef. GelderblomHans, Hauroeder Baerbel, Schmetz ChristeL Milios Jim,Hofstaedter Ferdinand

LS15b.01 Characterization ofEl Tor choleraphage SS by electron microscopy D1.347

Mitra Kalvan. GhoshA.N.

LS15b.02 The impacts of virus infection on the subcellular distribution and localization of glutathione in

CucurbitapepoL. JH.349

Zechmann Bernd. Muller M., Zellnig G.,

L,S15b.03 Electron microscopic rapid virus diagnosis of negative-stained urine samples canbe useful in the

diagnosis and monitoring of polyomavirus infections in renal transplant recipients LTJ.351

Schroeder Josef Kraemer Bernhard, Hofstaedter Ferdinand

LS15b.04 Ultrastructural analysis of sperms in patients with oligoasthenospermia JJJ.353

Jezek Davor. Colak Bozo, Romac Pavle, Balarin Maja, Barcot Ognjen

LS15b.05 A prospective study on the use of the molecular marker p^"1*'''1 as an adjunct in liquid-basedcervical cytology 1JJ.355

Boulet Gaelle. Sahebali Shaira, Depuydt Christophe, Moeneclaey Liliane, Vereecken Annie,VanMarckEric, Bogers Johannes

LS15.P01 Examination of the ultrastructural mechanisms ofpterygium HI.357

Huseyinova G.. Arda S.

LS15.P02 Viral gastroenteritis (SRSVs; Noro viruses and Sapo viruses) andnewly developed diagnostic electron

microscopy (automated specimens search system installed TEM) UI.359

Tajiri-Utagawa E.. Sanekata T.

LS15.P03 Investigation of cell ultrastructure and detection of hepatitis C virus RNA in liver biopsies of patientswith a hepatitis C by RT-PCR in situ UJ.3 61

Severova Anastasy. Zavalishina L., Manykin A., Lisitcin F., Kutcherova T.

xvn

LS15.P04 Theelectron microscopy as a reliable tool for rapid and conventional detection of enteric viral agents:a five-year experience report 0.363

Arcangeletti Maria Cristina. DeConto Flora, Pinardi Federica, Medici Maria Cristina,

Valcavi Pierpaolo, Ferraglia Francesca, Motta Federica, Calderaro Adriana, Chezzi

Carlo, Dettori Giuseppe

LS15.P05 Ultrastructural findings in sural nerve biopsy in the demyelinating disease. in.365

Acar Goksemin. Demir Needet, Idiman Egemen, Tanriover Gamze, Sati Leyla, Yaba Aylin,

Demir Ramazan

LS15.P06 Detection of Viral agents causing gastroenteritis among children in Nairobi, Kenyabyelectron microscopy III.367

Nvanpao James. OkothF.A.

LS15.P07 Effect of temperature on expression ofAQP4 and Double-layered membrane structure of glial lamella III.369

Nishikawa Kouki. Hiroaki Yoko, Tani Kazutoshi, Fujiyoshi Y.

LS15.P08 Ultrastructural aspects of adaptation in pathogenic bacteria under different ecological conditions IH.371

Somova Larisa. Isachenko Anna, Busoleva Love, Somov Georgii, Pustovalov Eugenii

LS15.P09 The electron microscopy and atomic-forcemicroscopy study of the structure ofhuman fibroblasts

infected by the viruses of Togaviridae family m.373

Manvkin Anatolv. Lisitcin F.V., Guschina E.A., Efimov A.E., Cilinscy Y.Y., Yakovleva

B.A.

LS15.P10 Chronic hepatitis C infection in intravenous drug addicts: ultrastructural andimmunohistochemical

study HI.375

Makarova Natalv. Groma V., Zalcmane V., Gapeshina H., Capligina V., Jeruma A., Viksna

L., Rozentale B.

LS15.P11 Is accreditation necessary in EM viral diagnosis ? IH.377

Richter Susanne

LS15.P12 Oxidative stress and adhesion formation in osteoarthritic temporomandibular joint JH.379

LiemRobert S.B.. Stokroos I., Dijkgraaf L.C., Cordewener F.W., de Bont L.G.M., Zardeneta

G., Schmitz J., Milam S.B.

LS15.P13 Rapid diagnosis of viral infection by electron microscopy and simple latex agglutination test III.381

Sanekata Takeshi. Utagawa E.

LS15.P14 Bimoclomol attenuates mercuric chloride - induced nephrotoxicity in rat: an ultrastructural and

immunohistochemical m.383

Stacchiotti Alessandra. Lavazza A, Borsani E., Rezzani R., Rodella L., Bianchi R.

LS15.P15 Kangaroo aortic valves are histologicallymore similar to humanaortic valves: a comparative studyof kangaroo andporcine aortic valves HI.385

Waevtens Anouk, Narine Kishan, Forsyth Ramses, Cornelissen Maria, Roels Frank, Praet

Marleen, Van Nooten Guido

LS15.P16 Ultrastructural study of phagocytes in pseudotuberculosis 1H.387

PlelchovaNatalva. Somova Larisa, Dolzhikov Sergey

LS15.P17 The localization of Hantavirus in resident macrophages HI.389

Plekhova Natalya, SomovaLarisa. Pustovalov Eugenii, Kompanets Galina, Slonova Raisa

LS 16. Sample preparation in life sciences (Arvid B. Maunsbach)

LS16.I1 Heat-induced antigen retrieval of epoxy sections for electron microscopy 111.393

Brorson Sverre-Hennine

LS16.I2 The fine structure of the liver sieve revised by using correlative sample preparation methods and

high-resolution microscopy UI.395

Braet Filip. Wisse Eddie

LS16.02 Wetting the appetite for electron microscopy: EM of fully wet samples JH.397

Behar V., Ezer S., Horowitz M., Nechushtan A., Sabban A., Vainshtein A., ZikOrv

LS16.03 Influence of tilting and substrate conductivity onwet-mode imaging of cell cultures in the

environmental scanning electron microscope (ESEM) IJJ..399

Mestres Pedro, PQtz Norbert, Schoning M.J., Laue Michael

LS16.04 Light elements in cells measured by energy dispersive electron probe X-ray microanalysisof cryosections in.401

Zierold Karl. Michel Jean, Balossier Gerard

LSI6.05 A new compacthigh pressure freezing device IJJ..403

Walther Paul. Buser Ch., Hagedorn M., Wohlwend M.

XVIII

LS16.06 Developments in the ultrastructuial analysis of amyloid P-pcptide fibril formation m.405

Otte-Holler Irene. Wilhelmus Micha M.M., Boelens Wilbur C, VerbeekMarcel M., de WaalRobert M.W.

LS16.P01 Shear forces promote neutrophil transendothelian migration triggered by endothelium-displayedchemoattractants.Phase-contrast videomicroscopy and transmission electron microscopy studies. m.407

Shinder Vera. Cinamon Guy, Alon Ronen

LS16.P02 Neurological mouse models for Friedreichataxia: neuronal death investigated by electron microscopy HI.409Simon Delphine, Gansmuller Anne. Reutenauer Laurence, Hergueux Josiane, Koenig Michel,

Puccio Helene

LS16.P03 An electron microscopic andimmuno histochemical study on thehepatic metastatic mechanism of

pancreaticcarcinoma ni.411Takahashi Ichiro. Takama Michiho, Takagi Kenji, Takada Tadataka, Ladhoflf Axel-M.

LS16.P04 X-raymicroanalysis of apical fluid in cultures ofnormal and cystic fibrosis human airwayepithelial cells M.413

Kozlova Inna. Nilsson Harriet, Roomans G.M.

LS16.P05 Combination ofimmunogold electron microscopy and transgene technology reveals principles for

membrane protein anchoring in neurons and astrocytes m.415

Guiord Karen Marie. Riber B., Amiry-Moghaddam M., Utvik J.K., Ottersen O.P.

LS16.P06 Phosphate buffers are usable as negative stains HI.417Massover William H.

LS16.P07 Scanning electron microscopy of the block-face HI.419Laue MichaeL Leis Birgit, Piitz Norbert, Mestres Pedro

LS16.P08 Reliability of Fluoro-Jade B using in the rat spinal cord ischemic degeneration: a quantitative study m.421Orenda5ova Judita. Kucharova K., Ondrejcak T., MartonElkova M., Racekova E.

LS16.P09 Immunolabeling andPTA staining ofbudded baculovirus IH.423

Nevsten Pernilla. Borg Jorgen, Karlsson Gunnel, Wallenberg Reine, Holm Cecilia

LS16.P10 New insight into urothelial umbrella cells as revealed by cryofixation and freeze substitution HI.425

Romih Rok. Jezernik K.

LS16.P11 TJltrastractural effects of Losartan on embryonic rat kidney IH.427

Inan Sevinc. Akil I., Nazikoglu A., Gurcu B., Giray G., Ozbilgin K., Muftuoglu S.

LS16.P13 Showing the complexityofhuman sweat glands requires the combination of different

microscopic approaches m.429

Wilke Katrin. Keil French J., Wittera Klaus-Peter, WepfRoger, Biel Stefan S.

LS16.P14 SEM evaluation ofbleaching effects on humanteeth using a replica technique IH.431

Dietz Wolfram. Kraft Ulrike, Glockmann Eike, Noren Jorgen, Hoyer Ingrid

LS16.P15 Composition of airway-surface liquid in mouse trachea collected with ion-exchange beads

determined by X-ray microanalysis HI.433

Kozlova Inna. Nilsson Harriet, Roomans G.M.

LS16.P16 X-ray microanalysis of airway-surface liquid in frozenhydrated trachea of normal andcystic fibrosis mice m.435

Kozlova Inna. Phillipson Mia, Roomans G.M.

LS 17. General life sciences (Karl Otto Greulich)

LS17.01 Neuroimmunoendocrinology: key role of microscopy for creation ofnew discipline HI.439

Kvetnoy Irot. Kvetnaia Tania, Polyakova V., Trofimov A., Konovalov S., Knyazkin I.

LS17.02 The effects of diethylcarbamazine on the ultrastructure of lung cells in vivo HI.441

Peixoto Christina. Saraiva KarinaLidianne Alcantara, Florencio Monica Sim5es

LS17.03 Morphological study of interleukin-10 producing Lactococcus lactis in mouse ileal loops HI.443

Waevtens Anouk. Neirynck Sabine, Waelput Wim, Rottiers Pieter, Steidler Lothar, Cuvelier

Claude

LS17.04 Ultrastructural study of age-related changes in the prostate of albino rats 10.445

Ghallab Avman. Gawish Magdy, SaaidHekmat, AbdEl-Haleim Manal

LS 17.05 Possible ultrastructural differences in placental vasculogenesis and angiogenesis during

early pregnancy EI.447

Demir Ramazan, Kayisli Umit Ali, Seval Yasemin, Celik - Ozenci Ciler, Korgun Emin

Turkay, Demir-Weusten Avse Yasemia Huppertz Berthold

IXX

LS17.06 The ultrastructureof the enamel ofmouse mandibular arch tooth grafts implanted under the

kidney capsule III.449

Klinge Randi Furseth. Sehic Amer, Risnes Steinar, From Sigurd H.

LS17.07 Pseudovacuolar inclusions located at the front ofWalker carcinosarcoma cells IQ.451

Vanhecke Dimitri. Eggli Peter, Keller Hansuli, Studer Daniel

LS17.08 On the interaction ofCC53Is colon carcinoma cells and liver sinusoidal cells duringmetastatis

using correlative microscopy 1H.453

Vekemans Katrien. Wisse Eddie, Braet Filip

LS17.09 Ultrastructural findings of excessive dietary - vitamin B6 (pyridoxine-hcl) intake on

cerebral cortex neurons in rat IH.455

Acar Goksemin. Sati Leyla, Tanriover Gamze, KayitJi Umit Ali, Demir Necdet, AgarAysel,Demir Ramazan

LS17.P01 The role of Fas / FasL and nitric oxide in the interaction between rat hepatic endothelial cells and

CC531s colon carcinoma cells: a microscopic approach 10.457

Vekemans Katrien, Wisse Eddie, Braet Filip

LS17.P02 Effect of Chelidonium majus extract in rat hepatic intoxication inducedby CCL(. tructural and

ultrastructural studies ni.459

Ardelean A.. Ciobanu C, Rusu M.A., Craciun C, Puica C, Tamas M.

LS17.P03 Protective effects ofa bioactive compoundtreatment onnew-born brain morphology and function

following aspartame ingestion during gestation in white rats m.461

Puica C, Craciun C. Ardelean A., Rusu M., Roman I.

LS17.P04 Structural-functional studies regarding the effect of Berberis vulgaris extract on rat intoxicated liver m.463

Craciun C. Ardelean A., Ciobanu C, RusuM.A., Puica C, Tamas M., Craciun V.

LS17.P05 Cell death in skeletal muscle culture cells m.465

Fraternale Manuela, Luchetti F., Ferri P., Burattini S., Battistelli M., Curci R., Falcieri E.

LS17.P06 Incorporation of exogenous lung surfactant material into air-blood barrier cell membranes m.467

Marszalek Andrzei. Seget Monika, FlorekEwa, Zabel Maciej, Biczysko Wieslawa

LS17.P07 Reverting senescence by low-dose stressors in bean leaves m.469

Nyitrai P., B6ka K., Sarviri E., Keresztes Aron

LS17.P08 Fine structure of dorsal skeletal muscle ofMexican axolotlAmbystoma mexicanum IJJ..471

Kevhani Ezzatollah

LS17.P09 Internalization mechanisms of apoptotic and necrotic L929 cells in an in vitro assay as studied byFACS, TEM, SEM andtime-lapse videomicroscopy HI.473

Krysko Dmitri, Gabriels S., Denecker G., Vandenabeele P., D'Herde Katharina

LS17.P10 Radial glial cells in mixed glial cell cultures ofnewborn rat spinal cord - morphological and

irnmunocytochemical characterization m.475

Moreels Marian. Neyens Wendy, Stinissen Piet, Lambrichts Ivo, Vandenabeele Frank

LS17.P11 The expression of the P2Xi receptor in the gastrointestinal tract ofthe mouse and guinea-pig IH.477

Van Nassauw Luc. De Jonge Frederik, Vanderwinden Jean-Marie, Burnstock Geoffrey,Timmermans Jean-Pierre

LS17.P12 Morphological aspect of brown adipocyte peroxisomes biogenesis in hypothyroid rats IH.479

Cakic-Milosevic Maja. Korac Aleksandra, Ukropina Mirela, Davidovic Vukosava

LS17.P13 Naphthoquinone cataract in mice: mitochondrial change and protection bysuperoxide dismutase m.481

Martinkina Larissa. Vengerov Y., Qian W., Shichi H.

LS17.P14 Fine structure of the human uterine luminal and glandular epithelium during early pregnancy JH.483

Demir Ramazan. Kayisli Umit Ali, Ozenci Ciler Celik

LS17.P15 Granulosa explants as in vitro model to study follicular atresia: implication of the extracellular

Ca2+-sensing receptor in inhibition of apoptosis JH.485

Mussche Sylvie, Espeel Marc, D'Herde Katharina

LS17.P16 Interaction of drug-sensitive and -resistant human melanoma cells with HUVEC cells UJ.487

Strinearo Annarita. Colone Marisa, Toccacieli Laura, Arancia Giuseppe, Molinari AgneseLS17.P17 Enrichment of isolated septal pore caps of the plant pathogen Rhizoctonia solani m.489

Van Driel Kenneth, Muller Willy, van Peer Arend, Verkleij Arie, Wosten Han, Boekhout

Teun

LS17.P18 Influence of experimental varicocele on the expression of interleukin-1 alpha in rat testis: an

immunohistochemicaland electron microscopic study H1.491

Sahin Zeliha. Akkoyunlu Gokhan, Seval Yasemin, Erdogru Tibet, Demir Ramazan, OstunelIsmail

XX

LS17.P19 Activation of pulmonary macrophages after exogenous surfactant treatment IH.493

Biczysko Wieslawa, Marszalek Andrzei. Seget Morrika, FlorekEwa

LS17.P20 Mitochondrial alterations inducedby Janus green B HJ.495

Kevhani Ezzatollah

LS17.P21 Schmorl's picro-thionin staining used to analyze dentinal structure in theNOS-3 knockout mouse UI.497

Strays Tom, Krage Tracy, Riider Stephan, Moreels Marjan, Vandenabeele Frank. Lambrichts

Ivo, Raab Wolfgang

LS 18. Round table: The human cytome project (Peter Van Osta)

Author list DJ 499

XXI