product presentation metris

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Product Presentation

Instruments for AnimalBehavioral Research

© Metris b.v. 2009

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ContentContent

Company overviewCompany overview

ProductsProducts

Importance of the energy parameter in Importance of the energy parameter in Behavioral PharmacologyBehavioral Pharmacology

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Company overviewCompany overviewGoalGoal

Provide turnkey solutions / expertise in the area ofProvide turnkey solutions / expertise in the area ofautomatic animal behavior analysis.automatic animal behavior analysis.

ProductsProducts MarketsMarketsLABORASLABORAS Pharmaceutical industry Pharmaceutical industrySonoTrackSonoTrack Research institutes/CRO’s Research institutes/CRO’sThird Party productsThird Party products Universities/ Academic Hospitals Universities/ Academic Hospitals

ActivitiesActivities Product Development, Product Development, Marketing / Sales , Marketing / Sales , Assembly and Product support. Assembly and Product support.

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HistoryHistory 5/19995/1999 Metris BV startMetris BV start 9/20019/2001 Delivery of first LABORAS for Delivery of first LABORAS for

Windows systemsWindows systems 2/20052/2005 Introduction SONOTRACK (Ultrasound Introduction SONOTRACK (Ultrasound

recording and analysis)recording and analysis) 7/20057/2005 Distributor SleepSign for Animals Distributor SleepSign for Animals

(EU)(EU) 8/20068/2006 Introduction LABORAS 2.0Introduction LABORAS 2.0 12/200812/2008 Introduction SonoTrack USV PlaybackIntroduction SonoTrack USV Playback 1/20091/2009 Introduction SonoTrack 2.0Introduction SonoTrack 2.0

Since 2001 Since 2001 ± 400± 400 platforms are delivered and platforms are delivered and over 50 references on our website support the over 50 references on our website support the value of LABORAS as experienced by researchersvalue of LABORAS as experienced by researchers

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Company RelationsCompany Relations

DistributorsDistributors Neuroscience Inc. (Japan)Neuroscience Inc. (Japan) Kuo Yang Scientific Corporation (Taiwan)Kuo Yang Scientific Corporation (Taiwan) Mirae Korea Inc. (South Korea) Mirae Korea Inc. (South Korea) Medi Analytika Ltd. (India)Medi Analytika Ltd. (India) AH Medical Supply (China) AH Medical Supply (China) Radon Medical (Turkey)Radon Medical (Turkey)

CooperationsCooperations DataSciences – Telemetry (USA)DataSciences – Telemetry (USA) Kissei (Japan)Kissei (Japan) Porsolt (France)Porsolt (France) National Instruments (USA, NL, Armenia)National Instruments (USA, NL, Armenia)

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LABORASLABORAS

LABORAS is a fully automatic LABORAS is a fully automatic system for continuous behavior system for continuous behavior recognition and tracking of small recognition and tracking of small rodents (mice and rats)rodents (mice and rats)

LABORASLABORAS replaces, reduces replaces, reduces and refines behavioral scoring by and refines behavioral scoring by human observershuman observers

LABORAS sets a new standard LABORAS sets a new standard in the area of behavioral in the area of behavioral observation instrumentsobservation instruments

What is LABORAS?What is LABORAS?

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1980 1990 2000 2010

Infrared beammatrix (8x5) Infrared beam

matrix (3D)

LABORAS2.0

Infrared beammatrix (4x3)

ANIMEX

Video tracking(single point) Video tracking

(multi point)

LABORAS1st. generation

Low

resolution

XY

track h

igh

0 no. of b

ehaviors >

10

Evolution of automatic tracking & behavioral observation systems

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LABORAS techniqueLABORAS technique (1/3)(1/3)

Main Main componentscomponents

LABORASLABORAS

Measurement Measurement platform(s)platform(s)

ElectronicsElectronics(e.g. amplifiers & (e.g. amplifiers & control & A/D control & A/D conv.)conv.)

LABORAS softwareLABORAS softwarefor Windowsfor Windows

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x

y

t

Behaviors

Behavior

Track

AdministrationA/D

conv.

Am

p. Ctr.

&Tracking

Results

ctr

ctr

Measurement platform(s)

Electronics(@ platform) PC + software

Graphs Tables

t behav

0

t x y

0.500.75

0.2510

49

3 10688

05.259.7515.5

immobeatdrinkgroom

Export

ASCII

e.g. MS-Excel

SPSS

SAS JMP

ElectronicControl

Unit

LABORAS technique LABORAS technique (2/3)(2/3)

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LABORAS technique LABORAS technique (3/3)(3/3)

Locomotion

Scratching

Example of recognition of behavioral Example of recognition of behavioral featuresfeatures

Fast up and down movement of the hindfoot, rubbing the side of the body, neck and face.

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Tracking measurementTracking measurement

Tracking information provided by LABORASTracking information provided by LABORAS

High resolution XY position trackHigh resolution XY position track Velocity data (average, max.)Velocity data (average, max.) Distance traveledDistance traveled Position distribution (details like favorite spots)Position distribution (details like favorite spots) Circling / turning (Clockwise and counter-clockwise circle Circling / turning (Clockwise and counter-clockwise circle

count, avg. circle size, avg. circling speed, avg. time in count, avg. circle size, avg. circling speed, avg. time in circle)circle)

Sociality Cage (preference to visit specific compartments Sociality Cage (preference to visit specific compartments in cage)in cage)

Estimation of energy use Estimation of energy use (in development)(in development)

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Detection of behaviorsDetection of behaviors

LABORAS distinguishes more than 16 LABORAS distinguishes more than 16 different rat and mouse behavioursdifferent rat and mouse behaviours

Normal behavioursNormal behaviours LocomotionLocomotion ImmobilityImmobility RearingRearing ClimbingClimbing EatingEating DrinkingDrinking GroomingGrooming

Special behavioursSpecial behaviours Scratching (mice)Scratching (mice) Seizures - tonic-clonic convulsions, Seizures - tonic-clonic convulsions,

barrel-rolls, Racine P4 and P5 barrel-rolls, Racine P4 and P5 (mice)(mice)

Hind limb licking / Formalin test Hind limb licking / Formalin test (rat)(rat)

Purposeless chewing (rat)Purposeless chewing (rat) Wet dog shakes (rat)Wet dog shakes (rat) Headshakes (rat)Headshakes (rat) Head twitches (rat)Head twitches (rat)

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LABORASLABORAS configurationsconfigurations

Configuration variablesConfiguration variables Up to to 8 measurement platforms connected to one Up to to 8 measurement platforms connected to one

computercomputer Species: mice, rat or bothSpecies: mice, rat or both Tracking and Behavior software modules:Tracking and Behavior software modules:

– TrackingTracking– Normal behaviors Normal behaviors – Special behaviors Special behaviors

Integration with Telemetry (Behavior & Psychology)Integration with Telemetry (Behavior & Psychology) Sociality Cage (special cage that enables sociability test)Sociality Cage (special cage that enables sociability test) User interface specialsUser interface specials

– GLPGLP– Independent start of the platformsIndependent start of the platforms

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Examples of resultsExamples of results

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LABORAS QualificationLABORAS Qualification (1/6)(1/6)

Operational Validation:Operational Validation:– Does LABORAS performs as advertisedDoes LABORAS performs as advertised– Explained in the following sheetsExplained in the following sheets

Pharmacological ValidationPharmacological Validation– Does LABORAS assist me with my researchDoes LABORAS assist me with my research– Metris role to assistMetris role to assist– E.g. new experiments or new algorithmsE.g. new experiments or new algorithms

CertificatesCertificates– GLP (Laboratory req.) , CE / FCC (electrical req.)GLP (Laboratory req.) , CE / FCC (electrical req.)

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Camera

Platform

Electronics

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timet

durationTestT

TatobserverstimecumulativeofmeancumTObs

tatobserverstimecumulativeofmeancumtObs

tatLABORAStimecumulativecumtLab

cumcumobscumcum TObsTtObsttObstLab

)(

)(

)(

)(%4%1)(%5)(5.1)()(

Grooming (Rat14) : 90%

0

100

200

300

400

500

600

700

800

0 500 1000 1500 2000 2500 3000 3500

Time (s)

Cu

mu

lat.

tim

e G

roo

min

g (

s)

LABORAS

Alex

Lee

Mean Obs

Boundary-

Boundary+

LABORAS Qualification LABORAS Qualification (3/6)(3/6)

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Immobility (Rat01): 90%

0

200

400

600

800

1000

1200

1400

1600

1800

0 600 1200 1800 2400 3000 3600

Time (s)

Cu

mu

lati

ve t

ime

(s)

LABORAS

Alex

Tania

Heleen

Mean Obs.

Bound -10%

Bound +10%

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Validation ResultsValidation Results● Correlation with human observers (according Correlation with human observers (according

to validation criterion)to validation criterion)– Locomotion, ClimbingLocomotion, Climbing > 95%> 95%– Immobility, Grooming Immobility, Grooming > 90%> 90%– Eating, Drinking, RearingEating, Drinking, Rearing > 80%> 80%

– Special behaviorsSpecial behaviors > 80-90%> 80-90%

● Since 2001 Since 2001 ± 400± 400 platforms are delivered and platforms are delivered and over 50 references on our website support the over 50 references on our website support the value of LABORAS as experienced by value of LABORAS as experienced by researchersresearchers

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Development of new algorithmsDevelopment of new algorithms• Good animal behavior definitionGood animal behavior definition• Proper definition of the experiment protocolProper definition of the experiment protocol

- and use of the LABORAS experiment administration- and use of the LABORAS experiment administration• Choice of pharmacological relevant behaviorsChoice of pharmacological relevant behaviors• Good correlation with the real behavior (acceptation Good correlation with the real behavior (acceptation

criterion)criterion)- Not always human observer reference- Not always human observer reference

• Results presentationResults presentation- Recording and Presentation of result data- Recording and Presentation of result data- Use of previous raw data/ result summaries- Use of previous raw data/ result summaries

Enabling the full benefit of LABORASEnabling the full benefit of LABORAS

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Examples of Examples of performanceperformance

Normal BehaviourNormal Behaviour (rat) (rat)

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Wet Dog Wet Dog ShakesShakes (rat) (rat)

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Head Head Twitches Twitches (rat) (rat)

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Circling Circling (rat)(rat)

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Sociality Cage (mice)Sociality Cage (mice)

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Applications LABORASApplications LABORAS LABORAS application areas are:LABORAS application areas are:

(behavioral assessment in)(behavioral assessment in)– Drug DevelopmentDrug Development –– Phenotyping Phenotyping – Safety PharmacologySafety Pharmacology –– Lab. animal science Lab. animal science– ToxicologyToxicology

Typical customers areTypical customers are::– Pharmaceutical CorpPharmaceutical Corp –– CRO’s (Contract Research Org.) CRO’s (Contract Research Org.)– Academic institutesAcademic institutes –– Lab. animal suppliers Lab. animal suppliers– HospitalsHospitals – – Governmental Governmental

Organizations Organizations

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Applications LABORASApplications LABORAS

Monitoring of behavioral changes and effects in:Monitoring of behavioral changes and effects in:

Drug Development Drug Discovery researchDrug Development Drug Discovery research– Efficacy studies of new compoundsEfficacy studies of new compounds– Long term observation tests (e.g. circadian rhytm,eat-Long term observation tests (e.g. circadian rhytm,eat-

drink patterns)drink patterns)– Experimentally induced behaviors (e.g. grooming, Experimentally induced behaviors (e.g. grooming,

drinking, movement patterns, hindlimb-licking / formalin-drinking, movement patterns, hindlimb-licking / formalin-test, scratching, WDS, Head Shakes)test, scratching, WDS, Head Shakes)

Safety PharmacologySafety Pharmacology ToxicologyToxicology Phenotyping researchPhenotyping research

– High Capacity Phenotyping with transgenic miceHigh Capacity Phenotyping with transgenic mice

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Applications LABORASApplications LABORAS Animal models based on behavioral assessment Animal models based on behavioral assessment

in home cage environmentin home cage environment– Normal behaviorsNormal behaviors– Drug induced behaviorsDrug induced behaviors

Pain studiesPain studies– Automated Formalin TestAutomated Formalin Test

Social InteractionSocial Interaction– Preference studies (with stranger and familiar mouse – Preference studies (with stranger and familiar mouse –

Sociality cage)Sociality cage) Animal welfare studiesAnimal welfare studies

– Cage enrichment studiesCage enrichment studies– Behavioral effects of manipulation of animalBehavioral effects of manipulation of animal

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Examples of LABORAS applications in drug discoveryExamples of LABORAS applications in drug discovery

TESTTEST RESULTRESULT

Behavioral phenotyping Behavioral phenotyping transgenic micetransgenic mice

Target discovery for future drug Target discovery for future drug developmentdevelopment

Primary observation testPrimary observation test Adverse drug effectsAdverse drug effects

Open field testOpen field test E.g. sedative, stimulant, E.g. sedative, stimulant, anxiolytic, anxiogenic propertiesanxiolytic, anxiogenic properties

Pain test (formaline induced Pain test (formaline induced hindlimb licking)hindlimb licking)

Analgesic propertiesAnalgesic properties

Dermatology test (scratching)Dermatology test (scratching) Skin protective effectsSkin protective effects

Telemetry & BehaviorTelemetry & Behavior Integrated cardiovascular, CNS Integrated cardiovascular, CNS and behavioral dataand behavioral data

Sociability TestSociability Test Analysis of social behavior (for Analysis of social behavior (for amongst other Autism research)amongst other Autism research)

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TESTTEST RESULTSRESULTS

Effect on Pharmacologically Effect on Pharmacologically induced effects, such as induced effects, such as • m-CPP hypolocomotion and m-CPP hypolocomotion and hypophagia hypophagia• 8-OH-DPAT hyperlocomotion8-OH-DPAT hyperlocomotion• d-Amphetamine hyperactivityd-Amphetamine hyperactivity• Angiotensin II polydypsiaAngiotensin II polydypsia• Orexin-A induced groomingOrexin-A induced grooming• Excessive climbingExcessive climbing• Turning/circling behaviorTurning/circling behavior• Wet Dog Shakes (WDS)Wet Dog Shakes (WDS)• Head Shake & Head TwitchHead Shake & Head Twitch• SeizureSeizure

Pharmacological efficacy of Pharmacological efficacy of (potential) leads(potential) leadsPharmacological antagonist Pharmacological antagonist efficacy, eg. anticonvulsant efficacy, eg. anticonvulsant activityactivity


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NEAR FUTURE APPLICATIONSNEAR FUTURE APPLICATIONS

Antagonism of pharmacologically Antagonism of pharmacologically induced effects, such as induced effects, such as DyskinesiaDyskinesia Startle responseStartle response

FUTURE APPLICATIONSFUTURE APPLICATIONS

RespirationRespiration Adverse effectsAdverse effects

Heart beatHeart beat Efficacy and safety issuesEfficacy and safety issues

Sniffing, Digging, JumpingSniffing, Digging, Jumping Detailed Exploration/Anxiety Detailed Exploration/Anxiety measuremeasure

Energy spendEnergy spend Metabolic effectsMetabolic effects

Disturbed feeding / drinking Disturbed feeding / drinking patterns (Eating / Drinking)patterns (Eating / Drinking)

Hypo- or hyperphagia; Hypo- or Hypo- or hyperphagia; Hypo- or polydipsiapolydipsia


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Overall benefits LABORASOverall benefits LABORAS

General benefits for customerGeneral benefits for customer● Standard and objective methodStandard and objective method

− Eliminates inter- and intra observer deviationEliminates inter- and intra observer deviation− Does not require trained or experienced ratersDoes not require trained or experienced raters

● Reduces time & costs (>75% reduction)Reduces time & costs (>75% reduction)● Results are almost immediately availableResults are almost immediately available● Many independent parameters can be Many independent parameters can be

measured with just one system (Tracking & measured with just one system (Tracking & behavior recognition combined in single behavior recognition combined in single system)system)

● Growth potential to determine several new Growth potential to determine several new behavioral and tracking parametersbehavioral and tracking parameters

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Overall benefits LABORASOverall benefits LABORAS

Important benefits for experimentImportant benefits for experiment● Independent of light conditionsIndependent of light conditions● Home cage environment and non-invasive Home cage environment and non-invasive

method (stress minimized)method (stress minimized)● Large scale observational experiments Large scale observational experiments

possiblepossible● Long test periods possibleLong test periods possible● More consistent resultsMore consistent results

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SONOTRACKSONOTRACK

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What is SONOTRACK?What is SONOTRACK? ● SONOTRACK records, visualizes and plays back SONOTRACK records, visualizes and plays back

Ultrasound CallsUltrasound Calls

● SONOTRACK records the SONOTRACK records the full spectrumfull spectrum from from 15kHz up to 100kHz 15kHz up to 100kHz

● SONOTRACK records SONOTRACK records 4 independent4 independent ultrasound ultrasound channelschannels

● SONOTRACK can easily be adjusted to SONOTRACK can easily be adjusted to experimental environment and is experimental environment and is easy to useeasy to use

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Ultrasonic VocalizationsUltrasonic Vocalizations Hearing range of Animals

ch

inch

illa

sh

eep

ho

rse

co

w racco

on

rab

bit

ferr

et d

og

hed

geh

og

gu

ineap

ig

gerb

il

cat

op

ossu

m

rat

mo

use

bat

belu

ga w

hale

po

rpo

ise

mo

th

1

10

100

1000

10000

100000

1000000

chin

chill

a

shee

p

hors

e

cow

raccoo

n

rabbit

ferr

et

dog

hedge

hog

guin

eapig

gerb

il

cat

opossum

rat

mouse

bat

belu

ga w

hale

porp

ois

e

moth

Fre

q. ra

ng

e (

Hz)

Log

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What is SONOTRACKWhat is SONOTRACK

● SONOTRACK can be used for long term SONOTRACK can be used for long term experimentsexperiments

● SONOTRACK recognizes and counts Ultrasonic SONOTRACK recognizes and counts Ultrasonic Vocalizations in user definable frequency Vocalizations in user definable frequency bandsbands

● SONOTRACK offers a new approach for SONOTRACK offers a new approach for characterization of animal behavior characterization of animal behavior

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SONOTRACK ApplicationsSONOTRACK Applications ● SONOTRACK can be used to monitor animal SONOTRACK can be used to monitor animal

welfare and interaction between animals welfare and interaction between animals based on the ultrasonic vocalizations of the based on the ultrasonic vocalizations of the animalsanimals

● Typical research areas:Typical research areas:– PainPain– AnxietyAnxiety– StressStress– DepressionDepression– Social interactionSocial interaction– Animal WelfareAnimal Welfare

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SONOTRACK ApplicationsSONOTRACK Applications ● Playback of Ultrasounds to the animal (stimulus)Playback of Ultrasounds to the animal (stimulus)

- playing back parts or complete recordings- playing back parts or complete recordings- playing back specific (artificial) frequency patterns- playing back specific (artificial) frequency patterns

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Examples of ResultsExamples of Results

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MarmosetMarmoset

Rats, Mother & PupRats, Mother & Pup

Ultrasound VocalizationsUltrasound Vocalizations

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Example of resultsExample of results

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What isWhat is SleepSign?SleepSign? Software for scoring of sleep stagesSoftware for scoring of sleep stages

Based on EMG and EEG signalsBased on EMG and EEG signals

Sleep stages can be scored in a manual mode Sleep stages can be scored in a manual mode as well as well as automaticallyas well as well as automatically

Scores pre-assigned sleep stages (such as REM, Scores pre-assigned sleep stages (such as REM, WAKE, SWS) and up to 3 user defined vigilance WAKE, SWS) and up to 3 user defined vigilance stagesstages

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Special featuresSpecial features Works with wireless or wired EMG and EEG Works with wireless or wired EMG and EEG

signalssignals In depth analysis using Hypnograms and Trend In depth analysis using Hypnograms and Trend

GraphsGraphs Various analysis functions, incl. Stage Graphs, Various analysis functions, incl. Stage Graphs,

Vigilance State Parameter Display, FFT analysisVigilance State Parameter Display, FFT analysis Integrated with VideoIntegrated with Video

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Application areasApplication areas

Pharmaceutical ResearchPharmaceutical Research

Sleep Disorder Studies and Sleep Sleep Disorder Studies and Sleep pattern Analysispattern Analysis

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Integration of SystemsIntegration of SystemsIntegration of systems such as LABORAS, Video, Integration of systems such as LABORAS, Video, DSI Telemetry, SonoTrack & SleepsignDSI Telemetry, SonoTrack & Sleepsign Less Experiments & Experimental lead timeLess Experiments & Experimental lead time

Less Resources (animals, researchers & money)Less Resources (animals, researchers & money)

Integrated analysis of parameters from different Integrated analysis of parameters from different domains collected from the same experiment domains collected from the same experiment

- Behavioral data and Tracking dataBehavioral data and Tracking data

- Psychological dataPsychological data

- SoundsSounds

- Sleep stagesSleep stages

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Integration of SystemsIntegration of Systems

With With SonotrackSonotrack

LABORASLABORAS With With SleepSignSleepSign

Example of integration in Laboras Example of integration in Laboras

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ENERGY & ENERGY & PHARMACOLOGYPHARMACOLOGY

Behavior and external / internal conditionsBehavior and external / internal conditions

Environment Environment -- External condition External condition

Process in the Body – Internal conditionProcess in the Body – Internal condition

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Basic structureBasic structure

BEHAVIOR = BEHAVIOR = Function { Internal stimulus, external stimulus} Function { Internal stimulus, external stimulus}

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Animal behavior and environmentAnimal behavior and environment

BEHAVIOR= Function {BEHAVIOR= Function {Process in the BodyProcess in the Body, nervous nervous system structure, nervous function, hormonal effects , system structure, nervous function, hormonal effects , biological clocks, perceptual mechanisms, Genes, biological clocks, perceptual mechanisms, Genes, stimulus filtering}stimulus filtering}

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External factor and environmental variables Temperature = function { temperature control Unit , CV }Temperature = function { temperature control Unit , CV }

Airflow = function air-conditioning}Airflow = function air-conditioning}Barometric pressure = function { place, time }Barometric pressure = function { place, time }

Humidity = function { office level or place }Humidity = function { office level or place }Visual effect = function { walking researchers in the laboratory}Visual effect = function { walking researchers in the laboratory}

Light = function { lights control unit}Light = function { lights control unit}Sounds = function { sounds generating equipment, voices } Sounds = function { sounds generating equipment, voices }

Vibration = function { refrigerators, ventilators, PCs, specific equipment } Vibration = function { refrigerators, ventilators, PCs, specific equipment } Elektromagnetic waves = function{ transmitting equipment, magnets}Elektromagnetic waves = function{ transmitting equipment, magnets}

Smell = function {specific gases, parfums, etc,}Smell = function {specific gases, parfums, etc,}

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Rule of energy preservation of the Rule of energy preservation of the bodybody

E = K + P = constantE = K + P = constant (Formula (Formula of energetic balance)of energetic balance)

Potential Energy of the BodyPotential Energy of the Body

P = ∆U+ P = ∆U+ δδ((εε − − TTηη + + PPνν))

-∆U -∆U is the change in biochemical energy is the change in biochemical energy - Ε- Ε is the internal energy is the internal energy

- T- Tηη is the entropy energy is the entropy energy - Pv - Pv is the thermodynamic energy is the thermodynamic energy

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THE IMPORTANCE OF THE THE IMPORTANCE OF THE ENERGYENERGY PARAMETER IN PARAMETER IN

BEHAVIORAL PHARMACOLOGYBEHAVIORAL PHARMACOLOGY

Kinetical energy of bodyKinetical energy of body

E (Locomotion) = Mv²/2E (Locomotion) = Mv²/2V V = velocity, M = total mass of body= velocity, M = total mass of body

E (Oscillation) = δm ω²R²/2E (Oscillation) = δm ω²R²/2ω = radial frequency, R = radius of oscillation,ω = radial frequency, R = radius of oscillation,δm = mass of oscillating body part δm = mass of oscillating body part

Mv²/2Mv²/2 δδmm²R²/2²R²/2++ E k =E k =

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THE IMPORTANCE OF THE THE IMPORTANCE OF THE ENERGYENERGY PARAMETER IN PARAMETER IN

BEHAVIORAL PHARMACOLOGYBEHAVIORAL PHARMACOLOGY Kinetical energy of bodyKinetical energy of body E (E (LocomotionLocomotion) ) is the kinetic energy is the kinetic energy

resulting from:resulting from: Locomotion, Walking, Rearing, ClimbingLocomotion, Walking, Rearing, Climbing

E (E (OscillationOscillation) ) is the kinetic energy is the kinetic energy resulting from different repetitive resulting from different repetitive movements:movements:

Scratching, Grooming , Chewing, Sniffing, Wet Dog Scratching, Grooming , Chewing, Sniffing, Wet Dog

Shakes, Head Shakes, Head Twitches, TremorsShakes, Head Shakes, Head Twitches, Tremors

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Detection, Detection, recognitionrecognition and and registration ofregistration of behaviorbehavior

Locomotion

ScratchingFast up and down movement of the hindfoot, rubbing the side of the body, neck and face.

Detection of behavioral featuresDetection of behavioral features

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LABORAS HS SignalLABORAS HS Signal

AnalogAnalog DigitalDigital

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E (E (OscillationOscillation) ) // E (E (LocomotionLocomotion) ) spectrogramspectrogram

Energy spectrogram

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10

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Imm

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Behavior

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Energy

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More information mail: [email protected] information mail: [email protected] BVMetris BV

Kruisweg 825Kruisweg 8252132 NG Hoofddorp2132 NG Hoofddorp

Phone:+31 23 5623400Phone:+31 23 5623400Fax:Fax: +31 23 5623425+31 23 5623425

www.metris.nlwww.metris.nl

Questions?Questions?

For Fast, Efficient & QualityFor Fast, Efficient & QualityPre-clinical ResearchPre-clinical Research

product presentation metris

Documents

introduction laboras

laboras software

value of laboras

d laboras

rats laboras

benefit of laboras

laboras qualification36

laboras technique23