pseudoneurospora canariensis - fungal planetneurospora calospora fgsc 958 neurospora terricola cbs...
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298 Persoonia–Volume32,2014
Pseudoneurospora canariensis
299Fungal Planet description sheets
© 2014 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
Etymology.Canariensis = from Canary Islands, Spain; referring to the geographicaloriginofthefungus.
Mycelium composed of subhyaline to brown, branched, septate, smooth-walled,thin-tothick-walledhyphae,1.5–9µmwide.Ascomata non-ostiolate, superficial to immersed, scatteredtogrouped,atfirstyellowishbrownandtranslucent,becom-ing dark yellowish brown to blackatmaturity, 120–220µmdiam,coveredbymoniliformhyphae,upto17µmwide;setaescarce, sinuose, smooth- and thick-walled, septate, brown, up to150µmlong,3–8µmatthebase,apexrounded;peridium3–4-layered, 5–15µm thick, brown, of textura angularis to globulosa, composed of brown, thick-walled, flattened cells, measuring13–35×12–23µm;ascomatalinitialsarisingassidebranches from mycelium, involving neighbouring hyphae, coiled and contorted.Paraphyses absent.Asci 8-spored, clavate,short-stipitate,200–300×20–25µm,withoutapicalstructures,soonevanescent.Ascospores uniseriate, aseptate, ellipsoidal, (30–)33–36×(18–)22–25µm,atfirstwithahyaline,smooth-walled exosporium and a pigmented, echinulate endosporium, becomingdarkbrown toblackatmaturity,opaque,stronglyverrucose to tuberculate, with a slightly protruding apical germ pore,1.5–2.5µmdiam. Culture characteristics — Colonies on potato carrot agar at-tainingadiameterofmorethan80mmin14dat25°C,velvetytopowdery,brown(5E5–5F5)(Kornerup&Wanscher1984);reversedarkbrown(8F4),blackatthemargins.Coloniesonoatmealagarattaining52–61mmdiamin14dat25°C,velvetytogranular,brown(5E5–5F5);reverseofthesamecolourthanthesurface.Thefungusdoesnotgrowbelow5°Cnorabove40°C.Optimalascomatalproductionoccurredbetween15°Cand25°C.
Typus.spaiN,GranCanaria,PicodeOsorio, from forestsoil,10Aug.2009,coll.M. Calduch & A.M. Stchigel,isol.Y. Marín(holotypeCBSH-21353,culturesex-typeCBS135818=FMR12156;otherstrainCBS136362=FMR12323;LSUsequencesofCBS135818andCBS136362GenBankHG326871andHG326872,respectively;TubsequencesofCBS135818andCBS136362GenBankHG423208andHG423209,respectively;andtef-1sequencesofCBS135818andCBS136362GenBankHG326875andHG326876,respectively;MycoBankMB804881).
Notes—Hitherto,Pseudoneurospora has been a monotypic genus(Garcíaetal.2004).ThemolecularanalysescarriedoutbyNygrenetal.(2011),basedonsevengeneregionsincludingfragmentsoftheβ-tubulin(Tub),translationelongationfactor1-α(tef-1),proteinkinaseC(pkc),28SrDNA,mitogen-activatedprotein kinase-2 (mak-2), nonidentical kinase-1 (nik-1) andhypotheticalprotein-coding(NCU02332)genes,corroboratedPseudoneurospora as a different genus to Neurospora.Ourphylogenetictreeobtainedbysequencingof28SrDNA,Tuband tef-1 loci corroborated our fungus as representing a new species of Pseudoneurospora.Pseudoneurospora canariensis can be distinguished from Pseudoneurospora amorphoporcata, the type species of the genus, by the production of small, non-ostiolateascomata(pyriformandostiolateinP. amorphoporcata),theabsenceofanapicalringintheasci(presentinthoseof P. amorphoporcata)andbythepresenceofasingleapicalgermporeon theascospores (oneateachend inP. amorphoporcata).
FungalPlanet278–10June2014
Pseudoneurospora canariensis Y.Marín,Stchigel,DaniaGarcía,Guarro&Cano,sp. nov.
Colour illustrations.PicodeOsorio,GranCanaria,Spain.Ascomata,ascusandascospores.Scalebars(top)=50µm,(bottom)=10µm.
YasminaMarin-Felix,AlbertoM.Stchigel,DaniaGarcia,JosepGuarro&JoséF.Cano-Lira,MycologyUnit,MedicalSchoolandIISPV,UniversityRoviraiVirgili,Reus,Spain;
e-mail: [email protected], [email protected], [email protected], [email protected] & [email protected]
Neurospora novoguineensis FMR 7269Neurospora retispora FMR 7510Neurospora santi-florii FGSC 8331
Neurospora dictyophora FMR 7511
Neurospora saitoi CBS 435.74T
Neurospora calospora FGSC 958Neurospora terricola CBS 298.63T
Neurospora sitophila FGSC 8770Neurospora tetrasperma FMR 5545
Neurospora crassa FGSC 8900Neurospora dodgei FGSC 1692T
Neurospora cerealis FGSC 959Neurospora hapsidophora CBS 408.82T
Neurospora reticulospora FGSC 6537Pseudoneurospora amorphoporcata CBS 626.80T
Pseudoneurospora canariensis CBS 135818T
Pseudoneurospora canariensis CBS 136362Sordaria fimicola FGSC 2918
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98/1
100/1
94/1
100/1
70/0.97
75/1
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Neurospora novoguineensis FMR 7269Neurospora retispora FMR 7510Neurospora santi-florii FGSC 8331
Neurospora dictyophora FMR 7511
Neurospora saitoi CBS 435.74T
Neurospora calospora FGSC 958Neurospora terricola CBS 298.63T
Neurospora sitophila FGSC 8770Neurospora tetrasperma FMR 5545
Neurospora crassa FGSC 8900Neurospora dodgei FGSC 1692T
Neurospora cerealis FGSC 959Neurospora hapsidophora CBS 408.82T
Neurospora reticulospora FGSC 6537Pseudoneurospora amorphoporcata CBS 626.80T
Pseudoneurospora canariensis CBS 135818T
Pseudoneurospora canariensis CBS 136362Sordaria fimicola FGSC 2918
100/1
98/1
100/1
94/1
100/1
70/0.97
75/1
99/1
100/1
97/1
83/0.97
91/1
0,01
-/1
Maximum likelihood tree obtained from the combined DNA sequencedatafromthreelociofourisolatesandsequencesretrievedfromtheGenBank(Nygrenetal.2011)(TreeBASEID14380).Inthetree,branchlengthsareproportionaltodistance.Bootstrapsupportvalues≥70/Bayesianposteriorprobabilityscores≥0.95are indicatedon thenodes.Sordaria fimicola (FGSC2918)wasusedasoutgroup.Ex-typestrainsofthedif-ferent species are indicated with T.Strainsofthenewspeciesproposed in this study are indicated in bold.