> Reality requires a chromatography solution that can be tailored to new needs

Whether your operation is small or large, one of the things that you know for sure is that your research

goals are likely to change. You need a solution that can be adapted as your needs evolve.

ÄKTA pure grows with your research

> Science needs protein purification to be easy

Regardless of your field of research, purified protein is often a necessity to accomplish your goals.

You need a solution that helps you spend less time on purifying proteins and more time focusing on the results.

ÄKTA pure lets you focus on your research

> In a competitive world, my chromatography system should just do the job

Research has never been as competitive as it is today. Protein purification is often one step of many in a much

larger research process. You need a solution you can rely upon to produce your pure proteins in time for when

they are needed.

ÄKTA pure builds on more than 50 years of protein purification expertise

There’s an ÄKTA pure for everyone Discover yours

Pure convenience

I need to get onwith my research

Pure cooperation

We’re all working on different projects

In our lab, we have to share the same chromatography system. None of us are purifying lots of protein, but we all need to do it at times. We need a system that’s easy to share and maintain together.

I develop assays that need pure proteins. I am not really comfortable purifying proteins, so I need a system that’s easy to work with.

For local office contact information, visit:

www.gelifesciences.com/contact

GE Healthcare Bio-Sciences AB

Björkgatan 30

751 84 Uppsala

Sweden

www.gelifesciences.com/aktapure

GE, imagination at work and GE monogram

are trademarks of General Electric Company.

ÄKTA and UNICORN are trademarks of GE

Healthcare companies.

© 2012 General Electric Company

All rights reserved.

First published Aug. 2012

GE Healthcare UK Limited

Amersham Place

Little Chalfont

Buckinghamshire, HP7 9NA

UK

GE Healthcare Europe, GmbH

Munzinger Strasse 5

D-79111 Freiburg

Germany

GE Healthcare Bio-Sciences Corp.

800 Centennial Avenue, P.O. Box 1327

Piscataway, NJ 08855-1327

USA

GE Healthcare Japan Corporation

Sanken Bldg., 3-25-1

Hyakunincho Shinjuku-ku

Tokyo 169-0073

Japan

Reverse-flow elution increases sample concentration Bypass for automation of cleaning Detection of standard proteins at 280 nm

Four inlet positions enable automatic system and column cleaning while running overnight

Fraction collection in tubes

We explore new proteins and want to learn more about their function. The most interesting proteins are often really difficult to purify. But has breaking new ground ever been easy? I need an efficient system to help me explore.

We purify mostly antibodies for our discovery programs, but also some drug targets and endogenous proteins. We are always looking to deliver more and keep costs down. What we need is more automation.

Pure automation

We need more efficiency

Pure exploration

Getting my proteins as pure as I need them is a real challenge

Completely load multiple samples automatically

Connect up to five columns, with automatic switching among them, with integrated pressure sensors for column protection

Detect any biomolecule with triple wavelength UV detection

Select multiple buffers and wash solutions with 14 inletswith integrated air sensors for system protection

Control gradients and elution conditions with pH and conductivity monitors

Use tubes or plates for flexible, high capacity fraction collection

ÄKTA™ pureProtein purification your way

GE HealthcareLife Sciences

> Intuitive and easy to use

Whether you are an expert or an absolute beginner in protein purification, ÄKTA pure makes routine protein purification easy.

The flow path is forward facing and organi-zed so that it is easy to understand how buffers and samples flow.

Convenient valves enable basic automation such as running overnight and including automatic cleaning steps.

> Fast method set-up

ÄKTA pure works with any column, but if you use one of ours, the method to run it is already defined in UNICORNTM 6 software.

If you would like to customize your method, it is easily achieved using the drag-and-drop interface.

For direct and full control of your runs, the software provides a user-friendly, interactive process picture.

> Add functionality when you need it

What if your next project takes an unexpec-ted turn to, for example, require the use of more automation or the use of a special detection method?

Relax. With the flexibility provided by ÄKTA pure in terms of valves, detectors, pumps, and software you can add almost any functionality you need to overcome any purification challenge.

If you need to run at low temperature, ÄKTA pure is small enough to fit inside a standard cold cabinet.

> Go quickly from one purification task to the next

With ÄKTA pure, turnaround from one purification task to another can be done in only a few minutes.

With a bypass function for your column and with multiple buffer inlet positions, cleaning can be automated to make sure the system is ready-to-use immediately after a run.

Changing sample application method from, for example, direct loading to injection from a loop is now simply done by a few clicks of a mouse due to the convenient injection valve.

> UNICORN on every computer

Because UNICORN 6 licenses are for concur-rent users, you can to freely install and access the software on as many computers as you like.

Add licenses as your usage of the software increases. Further, results and methods are easily shared within the team via the UNICORN central database.

The optional UNICORN column logbook keeps track of column performance and important run data, providing traceability and increased operational security, especially if columns are shared.

> Convenient, prepacked columns

We provide a vast selection of columns prepacked with our modern chromatogra-phy media providing solutions for conve-nient purification of tagged proteins and antibodies, but also gel filtration, IEX and HIC techniques, to match your varying needs for speed, purity, and yield.

The so called “crude concept” column design simplifies your workflow by enabling purification directly from crude, homogeni-zed, unclarified cell lysate.

> Automate the task

With ÄKTA pure, automation possibilities are endless—multiple buffer inlet positions, safe loading of up to seven samples completely, the ability to automatically switch between up to five columns, and flexibility to allow automatic two-step purification.

Add to that a fraction collector that can collect in tubes as well as microplates and ÄKTA pure will fit any purification workflow.

> From one system to a whole facility

UNICORN is a software platform that is common to ÄKTA systems from research- to process scale. The software can run in a network such that methods and data can be shared between all users and systems.

Sharing provides full flexibility and enables optimal use of available systems.

With UNICORN software and ÄKTA pure systems running in a network, increasing capacity by adding more systems is easy.

> Run unattended with confidence

ÄKTA pure automation valves have been designed to maximize your productivity.

Failures are eliminated with inlets safeguar-ded by integrated air sensors.

Columns are protected from overpressure by integrated pressure sensors controlling the flow.

Should a system alarm go off or if you simply want to know when the run is completed, UNICORN 6 can automatically send an e-mail notification so that you can take immediate action.

> Screening for optimal conditions made easy

Screening for optimal chromatography conditions is an important first step to maximize protein purity.

ÄKTA pure column selection valve allows automatic screening on multiple columns.

The loop valve enables, for example, scree-ning using the same sample in multiple conditions, and multiple buffer inlets can be used to screen for optimal buffer conditions.

> Detection to verify and evaluate any method

Several monitors to suit your application can be fitted to ÄKTA pure.

Up to two UV monitors can be combined. The pH valve in combination with the conducti-vity monitor gives you full verification of elution conditions.

Further, easy connection of external monitors for, for example, fluorescence, refractive index, and light scattering detec-tion applications greatly expands your possibilities.

> Integrated Design of Experiments (DoE)

DoE provides a structured approach to method optimization.

Using this optional feature in UNICORN 6 software, it is not only possible to plan an experimental series to maximize the amount of information obtained from a minimum number of experiments; the system can also run it in an automated fashion.

Once the experiment has been run, the results are used to create statistical models to determine the optimal chromatography conditions to be used going forward.

> Reality requires a chromatography solution that can be tailored to new needs

Whether your operation is small or large, one of the things that you know for sure is that your research

goals are likely to change. You need a solution that can be adapted as your needs evolve.

ÄKTA pure grows with your research

> Science needs protein purification to be easy

Regardless of your field of research, purified protein is often a necessity to accomplish your goals.

You need a solution that helps you spend less time on purifying proteins and more time focusing on the results.

ÄKTA pure lets you focus on your research

> In a competitive world, my chromatography system should just do the job

Research has never been as competitive as it is today. Protein purification is often one step of many in a much

larger research process. You need a solution you can rely upon to produce your pure proteins in time for when

they are needed.

ÄKTA pure builds on more than 50 years of protein purification expertise

There’s an ÄKTA pure for everyone Discover yours

Detection of standard proteins at 280 nm

> Reality requires a chromatography solution that can be tailored to new needs

Whether your operation is small or large, one of the things that you know for sure is that your research

goals are likely to change. You need a solution that can be adapted as your needs evolve.

ÄKTA pure grows with your research

> Science needs protein purification to be easy

Regardless of your field of research, purified protein is often a necessity to accomplish your goals.

You need a solution that helps you spend less time on purifying proteins and more time focusing on the results.

ÄKTA pure lets you focus on your research

> In a competitive world, my chromatography system should just do the job

Research has never been as competitive as it is today. Protein purification is often one step of many in a much

larger research process. You need a solution you can rely upon to produce your pure proteins in time for when

they are needed.

ÄKTA pure builds on more than 50 years of protein purification expertise

There’s an ÄKTA pure for everyone Discover yours

Pure convenience

I need to get onwith my research

Pure cooperation

We’re all working on different projects

In our lab, we have to share the same chromatography system. None of us are purifying lots of protein, but we all need to do it at times. We need a system that’s easy to share and maintain together.

I develop assays that need pure proteins. I am not really comfortable purifying proteins, so I need a system that’s easy to work with.

For local office contact information, visit:

www.gelifesciences.com/contact

GE Healthcare Bio-Sciences AB

Björkgatan 30

751 84 Uppsala

Sweden

www.gelifesciences.com/aktapure

GE, imagination at work and GE monogram

are trademarks of General Electric Company.

ÄKTA and UNICORN are trademarks of GE

Healthcare companies.

© 2012 General Electric Company

All rights reserved.

First published Aug. 2012

GE Healthcare UK Limited

Amersham Place

Little Chalfont

Buckinghamshire, HP7 9NA

UK

GE Healthcare Europe, GmbH

Munzinger Strasse 5

D-79111 Freiburg

Germany

GE Healthcare Bio-Sciences Corp.

800 Centennial Avenue, P.O. Box 1327

Piscataway, NJ 08855-1327

USA

GE Healthcare Japan Corporation

Sanken Bldg., 3-25-1

Hyakunincho Shinjuku-ku

Tokyo 169-0073

Japan

Reverse-flow elution increases sample concentration Bypass for automation of cleaning Detection of standard proteins at 280 nm

Four inlet positions enable automatic system and column cleaning while running overnight

Fraction collection in tubes

We explore new proteins and want to learn more about their function. The most interesting proteins are often really difficult to purify. But has breaking new ground ever been easy? I need an efficient system to help me explore.

We purify mostly antibodies for our discovery programs, but also some drug targets and endogenous proteins. We are always looking to deliver more and keep costs down. What we need is more automation.

Pure automation

We need more efficiency

Pure exploration

Getting my proteins as pure as I need them is a real challenge

Completely load multiple samples automatically

Connect up to five columns, with automatic switching among them, with integrated pressure sensors for column protection

Detect any biomolecule with triple wavelength UV detection

Select multiple buffers and wash solutions with 14 inletswith integrated air sensors for system protection

Control gradients and elution conditions with pH and conductivity monitors

Use tubes or plates for flexible, high capacity fraction collection

ÄKTA™ pureProtein purification your way

GE HealthcareLife Sciences

> Intuitive and easy to use

Whether you are an expert or an absolute beginner in protein purification, ÄKTA pure makes routine protein purification easy.

The flow path is forward facing and organi-zed so that it is easy to understand how buffers and samples flow.

Convenient valves enable basic automation such as running overnight and including automatic cleaning steps.

> Fast method set-up

ÄKTA pure works with any column, but if you use one of ours, the method to run it is already defined in UNICORNTM 6 software.

If you would like to customize your method, it is easily achieved using the drag-and-drop interface.

For direct and full control of your runs, the software provides a user-friendly, interactive process picture.

> Add functionality when you need it

What if your next project takes an unexpec-ted turn to, for example, require the use of more automation or the use of a special detection method?

Relax. With the flexibility provided by ÄKTA pure in terms of valves, detectors, pumps, and software you can add almost any functionality you need to overcome any purification challenge.

If you need to run at low temperature, ÄKTA pure is small enough to fit inside a standard cold cabinet.

> Go quickly from one purification task to the next

With ÄKTA pure, turnaround from one purification task to another can be done in only a few minutes.

With a bypass function for your column and with multiple buffer inlet positions, cleaning can be automated to make sure the system is ready-to-use immediately after a run.

Changing sample application method from, for example, direct loading to injection from a loop is now simply done by a few clicks of a mouse due to the convenient injection valve.

> UNICORN on every computer

Because UNICORN 6 licenses are for concur-rent users, you can to freely install and access the software on as many computers as you like.

Add licenses as your usage of the software increases. Further, results and methods are easily shared within the team via the UNICORN central database.

The optional UNICORN column logbook keeps track of column performance and important run data, providing traceability and increased operational security, especially if columns are shared.

> Convenient, prepacked columns

We provide a vast selection of columns prepacked with our modern chromatogra-phy media providing solutions for conve-nient purification of tagged proteins and antibodies, but also gel filtration, IEX and HIC techniques, to match your varying needs for speed, purity, and yield.

The so called “crude concept” column design simplifies your workflow by enabling purification directly from crude, homogeni-zed, unclarified cell lysate.

> Automate the task

With ÄKTA pure, automation possibilities are endless—multiple buffer inlet positions, safe loading of up to seven samples completely, the ability to automatically switch between up to five columns, and flexibility to allow automatic two-step purification.

Add to that a fraction collector that can collect in tubes as well as microplates and ÄKTA pure will fit any purification workflow.

> From one system to a whole facility

UNICORN is a software platform that is common to ÄKTA systems from research- to process scale. The software can run in a network such that methods and data can be shared between all users and systems.

Sharing provides full flexibility and enables optimal use of available systems.

With UNICORN software and ÄKTA pure systems running in a network, increasing capacity by adding more systems is easy.

> Run unattended with confidence

ÄKTA pure automation valves have been designed to maximize your productivity.

Failures are eliminated with inlets safeguar-ded by integrated air sensors.

Columns are protected from overpressure by integrated pressure sensors controlling the flow.

Should a system alarm go off or if you simply want to know when the run is completed, UNICORN 6 can automatically send an e-mail notification so that you can take immediate action.

> Screening for optimal conditions made easy

Screening for optimal chromatography conditions is an important first step to maximize protein purity.

ÄKTA pure column selection valve allows automatic screening on multiple columns.

The loop valve enables, for example, scree-ning using the same sample in multiple conditions, and multiple buffer inlets can be used to screen for optimal buffer conditions.

> Detection to verify and evaluate any method

Several monitors to suit your application can be fitted to ÄKTA pure.

Up to two UV monitors can be combined. The pH valve in combination with the conducti-vity monitor gives you full verification of elution conditions.

Further, easy connection of external monitors for, for example, fluorescence, refractive index, and light scattering detec-tion applications greatly expands your possibilities.

> Integrated Design of Experiments (DoE)

DoE provides a structured approach to method optimization.

Using this optional feature in UNICORN 6 software, it is not only possible to plan an experimental series to maximize the amount of information obtained from a minimum number of experiments; the system can also run it in an automated fashion.

Once the experiment has been run, the results are used to create statistical models to determine the optimal chromatography conditions to be used going forward.

> Reality requires a chromatography solution that can be tailored to new needs

Whether your operation is small or large, one of the things that you know for sure is that your research

goals are likely to change. You need a solution that can be adapted as your needs evolve.

ÄKTA pure grows with your research

> Science needs protein purification to be easy

Regardless of your field of research, purified protein is often a necessity to accomplish your goals.

You need a solution that helps you spend less time on purifying proteins and more time focusing on the results.

ÄKTA pure lets you focus on your research

> In a competitive world, my chromatography system should just do the job

Research has never been as competitive as it is today. Protein purification is often one step of many in a much

larger research process. You need a solution you can rely upon to produce your pure proteins in time for when

they are needed.

ÄKTA pure builds on more than 50 years of protein purification expertise

There’s an ÄKTA pure for everyone Discover yours

Pure convenience

I need to get onwith my research

Pure cooperation

We’re all working on different projects

In our lab, we have to share the same chromatography system. None of us are purifying lots of protein, but we all need to do it at times. We need a system that’s easy to share and maintain together.

I develop assays that need pure proteins. I am not really comfortable purifying proteins, so I need a system that’s easy to work with.

For local office contact information, visit:

www.gelifesciences.com/contact

GE Healthcare Bio-Sciences AB

Björkgatan 30

751 84 Uppsala

Sweden

www.gelifesciences.com/aktapure

GE, imagination at work and GE monogram

are trademarks of General Electric Company.

ÄKTA and UNICORN are trademarks of GE

Healthcare companies.

© 2012 General Electric Company

All rights reserved.

First published Aug. 2012

GE Healthcare UK Limited

Amersham Place

Little Chalfont

Buckinghamshire, HP7 9NA

UK

GE Healthcare Europe, GmbH

Munzinger Strasse 5

D-79111 Freiburg

Germany

GE Healthcare Bio-Sciences Corp.

800 Centennial Avenue, P.O. Box 1327

Piscataway, NJ 08855-1327

USA

GE Healthcare Japan Corporation

Sanken Bldg., 3-25-1

Hyakunincho Shinjuku-ku

Tokyo 169-0073

Japan

Reverse-flow elution increases sample concentration Bypass for automation of cleaning Detection of standard proteins at 280 nm

Four inlet positions enable automatic system and column cleaning while running overnight

Fraction collection in tubes

We explore new proteins and want to learn more about their function. The most interesting proteins are often really difficult to purify. But has breaking new ground ever been easy? I need an efficient system to help me explore.

We purify mostly antibodies for our discovery programs, but also some drug targets and endogenous proteins. We are always looking to deliver more and keep costs down. What we need is more automation.

Pure automation

We need more efficiency

Pure exploration

Getting my proteins as pure as I need them is a real challenge

Completely load multiple samples automatically

Connect up to five columns, with automatic switching among them, with integrated pressure sensors for column protection

Detect any biomolecule with triple wavelength UV detection

Select multiple buffers and wash solutions with 14 inletswith integrated air sensors for system protection

Control gradients and elution conditions with pH and conductivity monitors

Use tubes or plates for flexible, high capacity fraction collection

ÄKTA™ pureProtein purification your way

GE HealthcareLife Sciences

> Intuitive and easy to use

Whether you are an expert or an absolute beginner in protein purification, ÄKTA pure makes routine protein purification easy.

The flow path is forward facing and organi-zed so that it is easy to understand how buffers and samples flow.

Convenient valves enable basic automation such as running overnight and including automatic cleaning steps.

> Fast method set-up

ÄKTA pure works with any column, but if you use one of ours, the method to run it is already defined in UNICORNTM 6 software.

If you would like to customize your method, it is easily achieved using the drag-and-drop interface.

For direct and full control of your runs, the software provides a user-friendly, interactive process picture.

> Add functionality when you need it

What if your next project takes an unexpec-ted turn to, for example, require the use of more automation or the use of a special detection method?

Relax. With the flexibility provided by ÄKTA pure in terms of valves, detectors, pumps, and software you can add almost any functionality you need to overcome any purification challenge.

If you need to run at low temperature, ÄKTA pure is small enough to fit inside a standard cold cabinet.

> Go quickly from one purification task to the next

With ÄKTA pure, turnaround from one purification task to another can be done in only a few minutes.

With a bypass function for your column and with multiple buffer inlet positions, cleaning can be automated to make sure the system is ready-to-use immediately after a run.

Changing sample application method from, for example, direct loading to injection from a loop is now simply done by a few clicks of a mouse due to the convenient injection valve.

> UNICORN on every computer

Because UNICORN 6 licenses are for concur-rent users, you can to freely install and access the software on as many computers as you like.

Add licenses as your usage of the software increases. Further, results and methods are easily shared within the team via the UNICORN central database.

The optional UNICORN column logbook keeps track of column performance and important run data, providing traceability and increased operational security, especially if columns are shared.

> Convenient, prepacked columns

We provide a vast selection of columns prepacked with our modern chromatogra-phy media providing solutions for conve-nient purification of tagged proteins and antibodies, but also gel filtration, IEX and HIC techniques, to match your varying needs for speed, purity, and yield.

The so called “crude concept” column design simplifies your workflow by enabling purification directly from crude, homogeni-zed, unclarified cell lysate.

> Automate the task

With ÄKTA pure, automation possibilities are endless—multiple buffer inlet positions, safe loading of up to seven samples completely, the ability to automatically switch between up to five columns, and flexibility to allow automatic two-step purification.

Add to that a fraction collector that can collect in tubes as well as microplates and ÄKTA pure will fit any purification workflow.

> From one system to a whole facility

UNICORN is a software platform that is common to ÄKTA systems from research- to process scale. The software can run in a network such that methods and data can be shared between all users and systems.

Sharing provides full flexibility and enables optimal use of available systems.

With UNICORN software and ÄKTA pure systems running in a network, increasing capacity by adding more systems is easy.

> Run unattended with confidence

ÄKTA pure automation valves have been designed to maximize your productivity.

Failures are eliminated with inlets safeguar-ded by integrated air sensors.

Columns are protected from overpressure by integrated pressure sensors controlling the flow.

Should a system alarm go off or if you simply want to know when the run is completed, UNICORN 6 can automatically send an e-mail notification so that you can take immediate action.

> Screening for optimal conditions made easy

Screening for optimal chromatography conditions is an important first step to maximize protein purity.

ÄKTA pure column selection valve allows automatic screening on multiple columns.

The loop valve enables, for example, scree-ning using the same sample in multiple conditions, and multiple buffer inlets can be used to screen for optimal buffer conditions.

> Detection to verify and evaluate any method

Several monitors to suit your application can be fitted to ÄKTA pure.

Up to two UV monitors can be combined. The pH valve in combination with the conducti-vity monitor gives you full verification of elution conditions.

Further, easy connection of external monitors for, for example, fluorescence, refractive index, and light scattering detec-tion applications greatly expands your possibilities.

> Integrated Design of Experiments (DoE)

DoE provides a structured approach to method optimization.

Using this optional feature in UNICORN 6 software, it is not only possible to plan an experimental series to maximize the amount of information obtained from a minimum number of experiments; the system can also run it in an automated fashion.

Once the experiment has been run, the results are used to create statistical models to determine the optimal chromatography conditions to be used going forward.