P roduct iv it y. P recision. reliabil it y.

Today’s pharmaceutical laboratories are charged with moving thousands of samples through drug

discovery screening processes, all while maximizing return on investment in both time and resources.

You depend on automation to increase sample throughput and handle any sample format – tubes,

vials, and/or well plates – all while meeting the need to produce reliable results and identify leads.

Waters understands that when your lab is operating around the clock, keeping up with throughput

demand means every second in your system’s analysis time counts.

The Waters® Alliance® HPLC High Throughput (HT) System with integrated and automated sample

and solvent management is optimized for high efficiency separations, allowing valuable seconds to

be saved from each step of the chromatography process. The Alliance HPLC HT System is specifically

designed to improve the productivity of labs that require the selectivity and sensitivity of LC/MS

to produce high quality data.

The Alliance HPLC HT System features:

n Flexible modes of LC operation (sequential and parallel)

n Sample format versatility – plates, tubes, or vials

n Automated SystemPREP routine for rapid unattended system equilibration

n Purge-wash-purge capability for minimal sample carryover

alliance HPlc HiGH tHrouGHPut syst eM

tHe alliance Ht systeM can be conFiGured WitH:n e2795 separations Module

n optional alliance column

Heater or column

Heater/cooler

n a choice of high performance

optical and/or lc/Ms

detection offerings

n Masslynx™ or empower™

software

n a variety of leading HPlc consumables

The highly reproducible flow rates generated by the

Alliance HPLC HT System make it ideally suited

for coupling with mass spectrometers, such as the

Waters 3100 Mass Detector.

desiGned Wit H eFF ic iency in Mind

From sample prep, analysis, sensitivity, and quantitation hurdles, to uptime, compliance maintenance, and support concerns,

your Alliance HPLC HT System will allow you to meet these challenges and achieve the best performance and productivity for

your laboratory’s requirements.

choice of sample processing modes

Its integrated software and hardware design offers you the choice

of traditional sequential mode or parallel processing. Parallel

sample processing can reduce cycle time by performing multiple

chromatographic and system operations simultaneously. While

one sample is running, the next sample aspirates and loads; it is

immediately injected once the previous sample run is complete.

This accelerated injection-to-injection process greatly reduces cycle

time, and allows more samples to be analyzed in less time.

environmental control and sample stability

Controlling the environmental conditions of samples is

accomplished with a Peltier-based heating and cooling module,

which promotes sample stability, minimizes evaporation, and

stabilizes labile samples. The factory-installed sample heater/

cooler option maintains temperatures from 4 to 40 °C.

auto-process up to 1536 samples

The robotics-based three-axis (X, Y, Z) sample manager provides expanded

sample capacity for processing up to 1536 samples configured in four plates.

The instrument stores pre-programmed coordinates for standard microtiter plates,

or lets you input custom coordinates. To prevent needle damage and allow for

minimal sample volumes, a sensing routine determines the well or vial bottom.

And with rapid, automated initialization, system start-up is quick and easy.

dual solvent wash

The Alliance HPLC HT System utilizes two solvents to purge,

wash, and re-purge the needle assembly, loop, and injection

port. This reduces the risk of sample carryover and assures

assay integrity – critical for ADME and metabolite research –

making it ideal for high-sensitivity LC/MS/MS.

no tools are necessary

Primary components, such

as seals and pistons, can be

maintained by hand.

robust solvent management

The solvent management system blends up to four

chromatographic solvents in precise proportions for smooth,

pulse-free solvent flow delivery. This ensures that over

multiple runs, your results are precise time after time. The

PerformancePLUS™ In-line

Degasser efficiently removes

gases from the chromatographic

solvents. A fifth chamber in

the degasser handles purge

solvent used in the sample

management system.

automated valve switching

Change column methods, run multiple methods, or perform

unattended methods development with automated valve

switching, for up to three or six columns. Columns can be heated

or cooled in a Peltier-based forced air recirculation oven.

MaKinG ev ery second count

From sample processing to solvent management, the Alliance HPLC HT System has been designed to provide significant time savings by

enabling greater throughput along each step in your separation cycle. Key to this feature is being able to choose how you run your samples.

You can maximize throughput while maintaining control of your chromatographic separations. You can select among three processing options:

n sequential – Same as a traditional

chromatography separation cycle – each

function occurs in a step-by-step sequence.

n Parallel – Parallel processing offers an

advantage over traditional (sequential)

chromatography cycle times by performing

chromatographic operations in parallel.

Parallel processing maximizes throughput

by minimizing cycle time. While one sample

is running, a second sample can be drawn

and injected immediately after the preceding

injection is complete.

n Parallel with rapid equilibration – The system

provides for rapid equilibration by switching the

column off-line, purging the system at a high

flow rate, and then loading the next sample

during column re-equilibration. By using

parallel processing with rapid equilibration,

you can significantly increase throughput for

some applications.

Further processing improvements can be achieved by alternating the offline column regeneration. the use of two columns with the

system and a special regeneration valve, while incorporating parallel sampling allows for continuous data collection with no need

for additional re-equilibration time.

inJe

ct

seQuential

inJe

ctDELAY GRADIENT

needle wash

EQUILIBRATION

system and column sample load and draw

inJe

ct

Parallel

inJe

ctDELAY GRADIENT

needle wash

EQUILIBRATION

system and column sample load and draw

40% moresamples tested

inJe

ct

Parallel WitH raPid eQuilibration

inJe

ctGRADIENT

needle wash sample draw

RAPID EQUILIBRATION

sample load equilibrate column delay

Cycle Time = 5.1 min5 minutes run time188 runs in 24 hours

Cycle Time = 6.2 min5 minutes run time154 runs in 24 hours

Cycle Time = 4.4 min3.2 minutes run time218 runs in 24 hours

0 21 3 4 5 6 7 8 9 10 11

Minutes0 21 3 4 5 6 7 8 9 10 11

Minutes

0 21 3 4 5 6 7 8 9 10 11Minutes

Independently driven pistons achieve smooth solvent flow – without pulse dampeners – reducing gradient volume

Dual pressure transducers enable true pulse-free solvent flow

High-efficiency vacuum degassing technology

Precise solvent blending delivers highly reproducible gradients

Tool-free routine maintenance on plungers/seals/seal-wash seals

Simplified flow path with only two inlet check valves

Sample format versatilitySamples can be processed from standard

96 shallow- and deep-well plates, 384-well microtiter plates and custom plates,

2-mL vials, or 0.5- and 1.5-mL microcentrifuge tubes.

int eGrat ed Fluidics For consist ent PerForManc e and enHanc ed reP roducibil it y

The Waters e2795 Separations Module is the core of the system. Its integrated

solvent management and sample management provides consistent performance

and enhanced reproducibility for high-quality, high-throughput analysis for HPLC,

LC/MS, or flow injection-mass spectrometry applications.

solvent management

The simplified design of the e2795 Solvent Manager enables the blending and

delivering of solvents in a pulse-free, low-dispersion flow path.

In addition, Synchronized Composition Control™ (SCC™) of the gradient propor-

tioning valve (GPV) ensures that the e2795 Separations Module delivers excellent

gradient composition accuracy and precision.

The overall design results in reduced complexity and enhanced overall ruggedness

across a flow range that covers two orders of magnitude.

sample management

The design of the e2795 Sample Manager ensures precise sampling – delivering

predictable peak areas, and thus more consistent results. Complimenting the

simplified design of the solvent flow path, a user programmable needle wash, and

selectable “purge-wash-purge” cycle, ensures that any part of the system exposed

to sample is fully washed, virtually eliminating the risk of sample carryover.

The inherent versatility of the three-axis (X, Y, Z) needle assembly of the e2795

Sample Manager allows for greater applications flexibility. The sample manager

has a wide range of sample loops, holding loops, and syringe sizes available and

can process samples from a variety of plates, vials, and/or tubes. This variety

allows you to customize the configuration of the sample manager according to the

needs of your applications.

automated system start-up

The Alliance HPLC HT System features SystemPREP – a programmable, auto-

mated system preparation routine for fast, one-touch, walk-up operation. The

SystemPREP function performs a series of automated steps, putting the separa-

tions module in a condition of complete system readiness for running samples.

SystemPREP is useful when you are changing solvents or after extended periods

of system inactivity and is a simple-to-use alternative to running the individual

functions of Wet Prime, Purge Injector, Equilibrate, and Condition Column.

do More Wit H your results

Waters software solutions enable you to capitalize on the results

generated by your system. The Waters Laboratory Informatics Suite

includes proven information management solutions that integrate

all your lab resources in a consistent, scalable, and seamless

application environment.

Empower Software provides control of the system, including data

acquisition, management, processing, and reporting, all in the market-

leading chromatography data software (CDS) platform. Empower

Software is scalable from a single workstation to an enterprise-wide

CDS solution, and offers advanced features for method development,

reporting, and custom calculations, as well as Enterprise Options

for advanced data management and automated method validation

management.

MassLynx Software, for advanced mass spectrometry applications,

enables researchers to acquire, analyze, manage, and distribute

information. MassLynx Software offers flexible control over complex

instrument configurations, and Application Managers that are tailored

for specific LC/MS analyses.

NuGenesis® SDMS is a customizable scientific information

management platform that automatically captures and stores data in

a centralized data warehouse. SDMS makes information easier to access,

facilitates regulatory compliance, and aids in the administration of

intellectual property. SDMS converts scientific results into accessible

and valuable corporate knowledge.

be assured. cHoose Wat ers Global serv ic es.

With 94 offices in more than 50 countries, only the Waters Global

Services team has the most in-depth and up-to-date knowledge of the

advanced science and technologies that provide the foundation for

Alliance HPLC Systems. Maximize system uptime, increase laboratory

productivity, and meet stringent compliance requirements with the

expertise and responsiveness you need.

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Waters corporation 34 Maple StreetMilford, MA 01757 U.S.A.T: 508 478 2000F: 508 872 1990www.waters.com

Waters, Alliance, Symmetry, XTerra, and NuGenesis are registered trademarks of Waters Corporation. The Science of What’s Possible, MassLynx, Empower, PerformancePLUS, Synchronized Composition Control, and SCC are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2008 Waters Corporation. Produced in the U.S.A.October 2008 720000781EN LB-PDF