Quantitationpart 9

By : Saib Al Owini

• CELL COUNTING

- Hemocytometer

-Electronic Counting

PROTOCOL 21.1. CELL COUNTING BYHEMOCYTOMETER

OutlineTrypsinize a monolayer culture, or sample a suspension culture, prepare a

hemocytometer slide, and add the cells to the counting chamber. Count the cells on a microscope and calculate the cell concentration.

• Materials• Sterile:• D-PBSA• Crude trypsin, 0.25%• Growth medium• Yellow pipettor tips• Nonsterile:• Pipettor, 20 μL or adjustable 100 μL• Hemocytometer (Improved Neubauer)• Tally counter• Microscope

• H:\ ملفات YouTube - hemacytometer count- sabrina lin.flv\فيديوأنسجة

Electronic Counting

CASY Electronic Cell Counter.

CASY Electronic Cell Counter.

Beckman Coulter Vi-CELL Electronic Cell Counter

CELL PROLIFERATION

• Experimental Design

• Growth Cycle

Cytotoxicity

• Viability

•H:\ ملفات YouTube - Trypan Blue part 3.flv\فيديوأنسجة•H:\ ملفات YouTube - Trypan Blue part 2.flv\فيديوأنسجة•H:\ ملفات YouTube - Trypan Blue part 1.flv\فيديوأنسجة

Metabolic Cytotoxicity Assays

• Microtitration Assays